AlfalfaGEDB | MS.gene28620

M. sativa cultivar XinJiangDaYe / MS.gene28620

KEGG: -
GO: GO:0005575 GO:0005576 GO:0008150 GO:0009664 GO:0009987 GO:0016043 GO:0045229 GO:0071554 GO:0071555 GO:0071669 GO:0071840 GO:0110165
PFAM: PF01357.24 PF03330.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28620.t1	XP_003626098.1	100	173	0	0	11	183	82	254	5.10E-100	373.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28620.t1	Q9ZSI1	79.2	173	36	0	11	183	83	255	4.9e-83	308.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28620.t1	G7KVH3	100.0	173	0	0	11	183	82	254	3.7e-100	373.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050720	MS.gene28620	0.887651	1.18E-72	-1.69E-46
MS.gene050722	MS.gene28620	0.882232	1.24E-70	-1.69E-46
MS.gene050723	MS.gene28620	0.860848	1.55E-63	-1.69E-46
MS.gene050725	MS.gene28620	0.893462	6.15E-75	-1.69E-46
MS.gene050726	MS.gene28620	0.849848	2.48E-60	-1.69E-46
MS.gene050822	MS.gene28620	0.829803	4.20E-55	-1.69E-46
MS.gene050823	MS.gene28620	0.902085	1.39E-78	-1.69E-46
MS.gene053403	MS.gene28620	0.829081	6.28E-55	-1.69E-46
MS.gene053406	MS.gene28620	0.801104	1.05E-48	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28620.t1	MTR_7g111010	100.000	173	0	0	11	183	82	254	5.20e-129	362
MS.gene28620.t1	MTR_7g110990	100.000	173	0	0	11	183	67	239	1.29e-128	361
MS.gene28620.t1	MTR_1g085460	85.549	173	25	0	11	183	85	257	1.11e-110	316
MS.gene28620.t1	MTR_1g026020	63.584	173	62	1	11	183	84	255	1.86e-82	244
MS.gene28620.t1	MTR_5g075320	62.500	160	58	2	19	178	90	247	1.36e-72	219
MS.gene28620.t1	MTR_5g041700	62.805	164	58	3	20	183	87	247	3.16e-70	213
MS.gene28620.t1	MTR_3g435430	62.195	164	59	3	20	183	87	247	4.20e-70	213
MS.gene28620.t1	MTR_3g094040	62.893	159	57	2	19	177	92	248	5.84e-70	213
MS.gene28620.t1	MTR_3g094060	62.893	159	57	2	19	177	92	248	6.95e-70	213
MS.gene28620.t1	MTR_4g081950	62.658	158	57	2	20	177	123	278	8.17e-69	211
MS.gene28620.t1	MTR_2g097030	62.264	159	58	2	20	177	96	253	9.92e-69	210
MS.gene28620.t1	MTR_7g061720	58.929	168	65	3	11	177	87	251	1.83e-68	209
MS.gene28620.t1	MTR_5g079950	61.875	160	59	2	19	177	94	252	1.78e-67	206
MS.gene28620.t1	MTR_7g011060	61.635	159	51	3	19	177	87	235	5.23e-66	202
MS.gene28620.t1	MTR_3g466790	61.875	160	59	2	19	178	93	250	3.33e-65	201
MS.gene28620.t1	MTR_3g466760	61.875	160	59	2	19	178	99	256	1.85e-64	199
MS.gene28620.t1	MTR_4g063975	58.491	159	64	2	20	177	94	251	2.96e-60	188
MS.gene28620.t1	MTR_5g011210	54.878	164	71	3	22	183	100	262	6.97e-59	185
MS.gene28620.t1	MTR_4g124340	48.750	160	81	1	19	177	120	279	3.40e-58	184
MS.gene28620.t1	MTR_4g102450	55.769	156	67	2	29	183	107	261	8.51e-58	182
MS.gene28620.t1	MTR_6g012520	60.625	160	53	3	19	178	89	238	2.42e-57	180
MS.gene28620.t1	MTR_7g013140	54.217	166	72	3	19	183	89	251	4.75e-57	180
MS.gene28620.t1	MTR_0040s0140	53.459	159	72	2	21	178	95	252	1.45e-56	179
MS.gene28620.t1	MTR_7g013120	50.000	166	80	2	19	183	90	253	9.27e-56	176
MS.gene28620.t1	MTR_7g013150	51.807	166	76	3	19	183	91	253	1.26e-55	176
MS.gene28620.t1	MTR_2g090255	51.572	159	75	2	21	178	91	248	1.67e-54	173
MS.gene28620.t1	MTR_8g464350	50.602	166	78	3	19	183	92	254	1.05e-53	171
MS.gene28620.t1	MTR_8g058990	49.398	166	80	3	19	183	91	253	1.53e-51	166
MS.gene28620.t1	MTR_5g076950	49.057	159	80	1	20	178	103	260	1.39e-50	164
MS.gene28620.t1	MTR_3g064510	45.294	170	88	3	13	178	112	280	2.44e-47	156
MS.gene28620.t1	MTR_6g012500	58.333	96	39	1	82	177	181	275	3.55e-33	119
MS.gene28620.t1	MTR_0008s0120	46.018	113	60	1	71	183	88	199	8.06e-31	111
MS.gene28620.t1	MTR_1g085365	81.818	55	10	0	127	181	4	58	3.09e-26	95.5
MS.gene28620.t1	MTR_2g090270	30.539	167	95	5	27	183	103	258	1.64e-17	77.8
MS.gene28620.t1	MTR_2g090265	31.737	167	93	6	27	183	101	256	6.81e-16	73.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28620.t1	AT4G01630	79.191	173	36	0	11	183	83	255	3.05e-105	302
MS.gene28620.t1	AT1G20190	63.584	173	62	1	11	183	22	193	1.55e-79	235
MS.gene28620.t1	AT1G20190	63.584	173	62	1	11	183	81	252	3.36e-79	236
MS.gene28620.t1	AT2G40610	63.750	160	57	1	19	178	91	249	1.05e-73	222
MS.gene28620.t1	AT5G05290	60.625	160	62	1	19	178	93	251	2.12e-72	219
MS.gene28620.t1	AT1G69530	62.893	159	57	2	20	178	89	245	1.39e-69	212
MS.gene28620.t1	AT1G69530	62.893	159	57	2	20	178	89	245	1.39e-69	212
MS.gene28620.t1	AT1G69530	63.291	158	56	2	20	177	89	244	1.60e-69	213
MS.gene28620.t1	AT5G02260	60.248	161	62	2	19	178	93	252	5.01e-68	208
MS.gene28620.t1	AT2G03090	59.873	157	61	2	21	177	93	247	1.57e-67	206
MS.gene28620.t1	AT3G55500	60.625	160	61	2	19	177	95	253	1.83e-67	207
MS.gene28620.t1	AT5G56320	57.862	159	65	2	19	177	90	246	2.26e-66	204
MS.gene28620.t1	AT5G56320	57.862	159	65	2	19	177	90	246	2.26e-66	204
MS.gene28620.t1	AT2G39700	60.000	160	62	2	19	177	92	250	4.37e-66	203
MS.gene28620.t1	AT5G56320	57.862	159	65	2	19	177	90	246	7.99e-66	204
MS.gene28620.t1	AT2G28950	60.000	160	62	2	19	177	92	250	7.92e-65	200
MS.gene28620.t1	AT2G37640	58.385	161	65	2	19	178	72	231	1.71e-64	198
MS.gene28620.t1	AT3G29030	57.862	159	58	2	19	177	99	248	2.01e-64	199
MS.gene28620.t1	AT2G37640	58.385	161	65	2	19	178	97	256	2.13e-64	199
MS.gene28620.t1	AT3G15370	55.294	170	70	2	11	179	82	246	1.86e-63	196
MS.gene28620.t1	AT3G15370	55.294	170	70	2	11	179	112	276	5.14e-63	196
MS.gene28620.t1	AT1G26770	61.635	159	59	2	20	178	88	244	1.13e-60	189
MS.gene28620.t1	AT1G26770	61.635	159	59	2	20	178	98	254	1.25e-60	189
MS.gene28620.t1	AT1G12560	52.761	163	75	2	22	183	100	261	5.77e-57	180
MS.gene28620.t1	AT1G69530	64.122	131	43	3	20	150	89	215	2.53e-55	174
MS.gene28620.t1	AT1G69530	61.151	139	50	3	20	158	89	223	2.62e-55	175
MS.gene28620.t1	AT4G38210	51.875	160	75	2	20	178	92	250	2.39e-54	173
MS.gene28620.t1	AT5G39270	49.697	165	79	3	19	183	119	279	4.44e-54	173
MS.gene28620.t1	AT5G39310	48.851	174	82	5	11	183	145	312	3.10e-53	172
MS.gene28620.t1	AT5G39300	48.485	165	81	3	19	183	116	276	3.80e-53	171
MS.gene28620.t1	AT5G39280	47.879	165	82	3	19	183	115	275	9.81e-53	169
MS.gene28620.t1	AT5G39290	49.091	165	80	3	19	183	119	279	1.65e-52	169
MS.gene28620.t1	AT1G62980	50.000	164	79	3	22	183	94	256	3.18e-52	168
MS.gene28620.t1	AT3G03220	47.170	159	83	1	20	178	102	259	2.60e-49	160
MS.gene28620.t1	AT5G39260	43.030	165	85	3	19	183	107	262	4.32e-39	134
MS.gene28620.t1	AT1G65680	34.320	169	87	8	28	183	116	273	1.97e-16	75.1
MS.gene28620.t1	AT4G28250	29.167	168	97	5	27	183	104	260	6.73e-16	73.6
MS.gene28620.t1	AT2G20750	32.738	168	94	5	27	183	108	267	3.28e-15	72.0
MS.gene28620.t1	AT2G45110	32.000	175	89	8	25	183	99	259	4.84e-11	60.1

Find 0 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AACATAAGATTTACCATAAA+TGG	+	50573:19126-19145	MS.gene28620:CDS	20.0%
!!	ACATAAGATTTACCATAAAT+GGG	+	50573:19127-19146	MS.gene28620:CDS	20.0%
!!	TAACAATTTAATTGTGTGAT+CGG	-	50573:18792-18811	None:intergenic	20.0%
!!	TTAGAATTCAACTCAAAAAT+GGG	+	50573:19313-19332	MS.gene28620:CDS	20.0%
!!!	ATTTCTAAAGTTTGGATAAA+AGG	+	50573:19201-19220	MS.gene28620:CDS	20.0%
!!!	CTTAATTTTGATTAACTTGT+AGG	+	50573:18430-18449	MS.gene28620:intron	20.0%
!!!	TTTAGAATTCAACTCAAAAA+TGG	+	50573:19312-19331	MS.gene28620:CDS	20.0%
!!!	TTTCTAAAGTTTGGATAAAA+GGG	+	50573:19202-19221	MS.gene28620:CDS	20.0%
!	AAGGGTCAAAAAAGAATAAA+TGG	+	50573:19220-19239	MS.gene28620:CDS	25.0%
!	AGGGTCAAAAAAGAATAAAT+GGG	+	50573:19221-19240	MS.gene28620:CDS	25.0%
!	ATTAATTAAAGTGACACTAG+TGG	+	50573:18900-18919	MS.gene28620:intron	25.0%
!	GTATTTATATATGCAGAGTT+GGG	+	50573:19088-19107	MS.gene28620:intron	25.0%
!	TGTATTTATATATGCAGAGT+TGG	+	50573:19087-19106	MS.gene28620:intron	25.0%
!!!	AATTTTGATTAACTTGTAGG+TGG	+	50573:18433-18452	MS.gene28620:intron	25.0%
	AAAATAGTCTCTCCCATTTA+TGG	-	50573:19142-19161	None:intergenic	30.0%
	AATCTATACACAGATGGATA+TGG	+	50573:18472-18491	MS.gene28620:intron	30.0%
	AGTTTGACAATGAAACATCA+AGG	-	50573:18957-18976	None:intergenic	30.0%
	CTAAAAAATACTTGTTCGCT+AGG	-	50573:19006-19025	None:intergenic	30.0%
	GTTTGACAATGAAACATCAA+GGG	-	50573:18956-18975	None:intergenic	30.0%
	TATGGAAATCTATACACAGA+TGG	+	50573:18466-18485	MS.gene28620:intron	30.0%
!!	TAGTGGTCATTTTACAATGT+GGG	+	50573:18917-18936	MS.gene28620:intron	30.0%
!!!	AGCGAACAAGTATTTTTTAG+TGG	+	50573:19006-19025	MS.gene28620:intron	30.0%
	ACATACCTCCTGTACAAAAT+GGG	-	50573:18760-18779	None:intergenic	35.0%
	ACATATCAAAGTGAGGTCTA+GGG	-	50573:18690-18709	None:intergenic	35.0%
	AGTACTGCTCTCTTTAATGA+TGG	+	50573:18514-18533	MS.gene28620:intron	35.0%
	ATTTGTGGCAGTAATAGTGA+TGG	-	50573:18614-18633	None:intergenic	35.0%
	CAAAGCCTAAGCTATCTTAA+TGG	+	50573:19276-19295	MS.gene28620:CDS	35.0%
	GTACTGCTCTCTTTAATGAT+GGG	+	50573:18515-18534	MS.gene28620:intron	35.0%
	GTACTTAGCTATAGTCTGAA+AGG	-	50573:18722-18741	None:intergenic	35.0%
	TCTGACCATTAAGATAGCTT+AGG	-	50573:19284-19303	None:intergenic	35.0%
	TTAATGTTGCACCTTCCAAT+TGG	+	50573:19352-19371	MS.gene28620:CDS	35.0%
!	GAATTGTTCCCATTTTGTAC+AGG	+	50573:18749-18768	MS.gene28620:CDS	35.0%
!	GTTTGGTGGACAAAAATTTG+TGG	-	50573:18629-18648	None:intergenic	35.0%
!	TTGGTGCTTATAAGCAATGT+AGG	+	50573:19165-19184	MS.gene28620:CDS	35.0%
!	TTTAATGATGGGAAGTCATG+TGG	+	50573:18526-18545	MS.gene28620:intron	35.0%
!!	CTAGTGGTCATTTTACAATG+TGG	+	50573:18916-18935	MS.gene28620:intron	35.0%
!!	GTGGAGAGATTTCTAAAGTT+TGG	+	50573:19193-19212	MS.gene28620:CDS	35.0%
	ACCAACCACCATTGTCATTA+GGG	-	50573:18660-18679	None:intergenic	40.0%
	ACTATAGCTAAGTACAGAGC+TGG	+	50573:18727-18746	MS.gene28620:CDS	40.0%
	ATATCAAAGTGAGGTCTAGG+GGG	-	50573:18688-18707	None:intergenic	40.0%
	CAATTTACCCATTGTTCCTG+AGG	-	50573:18332-18351	None:intergenic	40.0%
	CATATCAAAGTGAGGTCTAG+GGG	-	50573:18689-18708	None:intergenic	40.0%
	CTTATAAGCAATGTAGGTGG+AGG	+	50573:19171-19190	MS.gene28620:CDS	40.0%
	GAACCGATGTCAATGAATTG+GGG	+	50573:19243-19262	MS.gene28620:CDS	40.0%
	GACAATGAAACATCAAGGGA+CGG	-	50573:18952-18971	None:intergenic	40.0%
	GACATACCTCCTGTACAAAA+TGG	-	50573:18761-18780	None:intergenic	40.0%
	GACATATCAAAGTGAGGTCT+AGG	-	50573:18691-18710	None:intergenic	40.0%
	GATTTGATTGATATGCAGCG+TGG	-	50573:19037-19056	None:intergenic	40.0%
	GGAACCGATGTCAATGAATT+GGG	+	50573:19242-19261	MS.gene28620:CDS	40.0%
	GGTTGAGACATATCAAAGTG+AGG	-	50573:18697-18716	None:intergenic	40.0%
	GTGCTTATAAGCAATGTAGG+TGG	+	50573:19168-19187	MS.gene28620:CDS	40.0%
	GTGTCAAACTATCAGTTCAG+TGG	-	50573:18984-19003	None:intergenic	40.0%
	TTAGCTATAGTCTGAAAGGC+AGG	-	50573:18718-18737	None:intergenic	40.0%
	TTTGCACTCCCTAATGACAA+TGG	+	50573:18649-18668	MS.gene28620:CDS	40.0%
!	ATCAGATAGTTTGTGACGCA+AGG	+	50573:18557-18576	MS.gene28620:intron	40.0%
!	CAATGAATTGGGGTGCTAAT+TGG	+	50573:19253-19272	MS.gene28620:CDS	40.0%
!	TCATTAGGGAGTGCAAAGTT+TGG	-	50573:18646-18665	None:intergenic	40.0%
!	TTGTTCCCATTTTGTACAGG+AGG	+	50573:18752-18771	MS.gene28620:CDS	40.0%
!!	TGGGAGAGACTATTTTGAGT+TGG	+	50573:19146-19165	MS.gene28620:CDS	40.0%
!!!	GGACGTTACTTTTGTAAGAC+TGG	-	50573:19386-19405	None:intergenic	40.0%
	AACTTGTAGGTGGTGCATGT+GGG	+	50573:18443-18462	MS.gene28620:intron	45.0%
	AAGTGATGCCTCAGGAACAA+TGG	+	50573:18321-18340	MS.gene28620:CDS	45.0%
	AGAGTTGGGTGCAAAAGAAG+TGG	+	50573:19102-19121	MS.gene28620:intron	45.0%
	AGTGATGCCTCAGGAACAAT+GGG	+	50573:18322-18341	MS.gene28620:CDS	45.0%
	ATAAGCAATGTAGGTGGAGG+TGG	+	50573:19174-19193	MS.gene28620:CDS	45.0%
	CACCAACCACCATTGTCATT+AGG	-	50573:18661-18680	None:intergenic	45.0%
	GCACCCCAATTCATTGACAT+CGG	-	50573:19249-19268	None:intergenic	45.0%
	GCACCTTCCAATTGGAGATT+TGG	+	50573:19360-19379	MS.gene28620:CDS	45.0%
	GGGAACCGATGTCAATGAAT+TGG	+	50573:19241-19260	MS.gene28620:CDS	45.0%
	TCCCTAATGACAATGGTGGT+TGG	+	50573:18656-18675	MS.gene28620:CDS	45.0%
	TGGCCAAATCTCCAATTGGA+AGG	-	50573:19366-19385	None:intergenic	45.0%
!	AATGATGGGAAGTCATGTGG+TGG	+	50573:18529-18548	MS.gene28620:intron	45.0%
!	AGACTGGCCAAATCTCCAAT+TGG	-	50573:19370-19389	None:intergenic	45.0%
!	TAACTTGTAGGTGGTGCATG+TGG	+	50573:18442-18461	MS.gene28620:intron	45.0%
!	TTAGGGAGTGCAAAGTTTGG+TGG	-	50573:18643-18662	None:intergenic	45.0%
	ACGCAAGGCAAGTTCCTCAA+TGG	+	50573:18572-18591	MS.gene28620:CDS	50.0%
	GCACTCCCTAATGACAATGG+TGG	+	50573:18652-18671	MS.gene28620:CDS	50.0%
	GTTCCTCAATGGTGTCTCAG+AGG	+	50573:18583-18602	MS.gene28620:CDS	50.0%
	GTAGGTGGTGCATGTGGGTA+TGG	+	50573:18448-18467	MS.gene28620:intron	55.0%
!!	GTGCCTCTGAGACACCATTG+AGG	-	50573:18589-18608	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
50573	gene	18314	19413	18314	ID=MS.gene28620
50573	mRNA	18314	19413	18314	ID=MS.gene28620.t1;Parent=MS.gene28620
50573	exon	18314	18343	18314	ID=MS.gene28620.t1.exon1;Parent=MS.gene28620.t1
50573	CDS	18314	18343	18314	ID=cds.MS.gene28620.t1;Parent=MS.gene28620.t1
50573	exon	18562	18773	18562	ID=MS.gene28620.t1.exon2;Parent=MS.gene28620.t1
50573	CDS	18562	18773	18562	ID=cds.MS.gene28620.t1;Parent=MS.gene28620.t1
50573	exon	19104	19413	19104	ID=MS.gene28620.t1.exon3;Parent=MS.gene28620.t1
50573	CDS	19104	19413	19104	ID=cds.MS.gene28620.t1;Parent=MS.gene28620.t1

Gene Sequence

>MS.gene28620

ATGGTGGAAGTGATGCCTCAGGAACAATGGGTAAATTGCTTCTTCTTTTGATTAACTTTATTCAAGTCTTAAGACAAATTAAAGTCTTAATCAAATTATGATAGTTAAGCATATAACTTAATTTTGATTAACTTGTAGGTGGTGCATGTGGGTATGGAAATCTATACACAGATGGATATGGTATAAAATCAGCAGCATTGAGTACTGCTCTCTTTAATGATGGGAAGTCATGTGGTGGTTGCTATCAGATAGTTTGTGACGCAAGGCAAGTTCCTCAATGGTGTCTCAGAGGCACTTCCATCACTATTACTGCCACAAATTTTTGTCCACCAAACTTTGCACTCCCTAATGACAATGGTGGTTGGTGTAATCCCCCTAGACCTCACTTTGATATGTCTCAACCTGCCTTTCAGACTATAGCTAAGTACAGAGCTGGAATTGTTCCCATTTTGTACAGGAGGTATGTCTTAATTTGCCGATCACACAATTAAATTGTTAATTAGAGTTTAATTTTGAAGCATGTTATTTTTTCAGTCAATTGATAAAAAAATCGTTGATTTTTAAATTAACGAAAAATAGAATAATTATTAATTAAAGTGACACTAGTGGTCATTTTACAATGTGGGATTTGAACTCCGTCCCTTGATGTTTCATTGTCAAACTTTTACCACTGAACTGATAGTTTGACACCTAGCGAACAAGTATTTTTTAGTGGATACACCACGCTGCATATCAATCAAATCTATAAATATGTGTTTTAAGCAATTTTTTGTTGTATTTATATATGCAGAGTTGGGTGCAAAAGAAGTGGAAACATAAGATTTACCATAAATGGGAGAGACTATTTTGAGTTGGTGCTTATAAGCAATGTAGGTGGAGGTGGAGAGATTTCTAAAGTTTGGATAAAAGGGTCAAAAAAGAATAAATGGGAACCGATGTCAATGAATTGGGGTGCTAATTGGCAAAGCCTAAGCTATCTTAATGGTCAGAGCTTATCTTTTAGAATTCAACTCAAAAATGGGAAGACTCGTACAGCTATTAATGTTGCACCTTCCAATTGGAGATTTGGCCAGTCTTACAAAAGTAACGTCCAGTTCTGA

Protein sequence

>MS.gene28620.t1

MVEVMPQEQWIVCDARQVPQWCLRGTSITITATNFCPPNFALPNDNGGWCNPPRPHFDMSQPAFQTIAKYRAGIVPILYRRVGCKRSGNIRFTINGRDYFELVLISNVGGGGEISKVWIKGSKKNKWEPMSMNWGANWQSLSYLNGQSLSFRIQLKNGKTRTAINVAPSNWRFGQSYKSNVQF