Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050723.t1 | XP_003626098.1 | 99.2 | 133 | 1 | 0 | 1 | 133 | 20 | 152 | 2.10E-74 | 288.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050723.t1 | Q9ZSI1 | 85.3 | 129 | 19 | 0 | 5 | 133 | 25 | 153 | 2.9e-64 | 245.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050723.t1 | G7KVH3 | 99.2 | 133 | 1 | 0 | 1 | 133 | 20 | 152 | 1.5e-74 | 288.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050720 | MS.gene050723 | 0.856819 | 2.48E-62 | -1.69E-46 |
| MS.gene050722 | MS.gene050723 | 0.878579 | 2.51E-69 | -1.69E-46 |
| MS.gene050723 | MS.gene050725 | 0.878539 | 2.59E-69 | -1.69E-46 |
| MS.gene050723 | MS.gene050726 | 0.979315 | 1.06E-147 | -1.69E-46 |
| MS.gene050723 | MS.gene050822 | 0.873367 | 1.55E-67 | -1.69E-46 |
| MS.gene050723 | MS.gene050823 | 0.888489 | 5.64E-73 | -1.69E-46 |
| MS.gene050723 | MS.gene072622 | 0.89798 | 8.30E-77 | -1.69E-46 |
| MS.gene050723 | MS.gene072623 | 0.862513 | 4.81E-64 | -1.69E-46 |
| MS.gene050723 | MS.gene072625 | 0.899834 | 1.34E-77 | -1.69E-46 |
| MS.gene050723 | MS.gene072626 | 0.860798 | 1.61E-63 | -1.69E-46 |
| MS.gene050723 | MS.gene072628 | 0.89907 | 2.85E-77 | -1.69E-46 |
| MS.gene050723 | MS.gene072629 | 0.863651 | 2.14E-64 | -1.69E-46 |
| MS.gene050723 | MS.gene28619 | 0.887471 | 1.39E-72 | -1.69E-46 |
| MS.gene050723 | MS.gene28620 | 0.860848 | 1.55E-63 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTTAATTTTGATTAACTTGT+AGG | - | 50569:13531-13550 | MS.gene050723:CDS | 20.0% |
| !!! | AATTTTGATTAACTTGTAGG+TGG | - | 50569:13534-13553 | MS.gene050723:CDS | 25.0% |
| AATCTATACACAGATGGATA+TGG | - | 50569:13573-13592 | MS.gene050723:CDS | 30.0% | |
| TATGGAAATCTATACACAGA+TGG | - | 50569:13567-13586 | MS.gene050723:CDS | 30.0% | |
| ACATACCTCCTGTACAAAAT+GGG | + | 50569:13861-13880 | None:intergenic | 35.0% | |
| ACATATCAAAGTGAGGTCTA+GGG | + | 50569:13791-13810 | None:intergenic | 35.0% | |
| AGTACTGCTCTCTTTAATGA+TGG | - | 50569:13615-13634 | MS.gene050723:CDS | 35.0% | |
| ATTTGTGGCAGTAATAGTGA+TGG | + | 50569:13715-13734 | None:intergenic | 35.0% | |
| GTACTGCTCTCTTTAATGAT+GGG | - | 50569:13616-13635 | MS.gene050723:CDS | 35.0% | |
| GTACTTAGCTATAGTCTGAA+AGG | + | 50569:13823-13842 | None:intergenic | 35.0% | |
| ! | GAATTGTTCCCATTTTGTAC+AGG | - | 50569:13850-13869 | MS.gene050723:CDS | 35.0% |
| ! | GTTTGGTGGACAAAAATTTG+TGG | + | 50569:13730-13749 | None:intergenic | 35.0% |
| ! | TTTAATGATGGGAAGTCATG+TGG | - | 50569:13627-13646 | MS.gene050723:CDS | 35.0% |
| ACCAACCACCATTGTCATTA+GGG | + | 50569:13761-13780 | None:intergenic | 40.0% | |
| ACTATAGCTAAGTACAGAGC+TGG | - | 50569:13828-13847 | MS.gene050723:CDS | 40.0% | |
| ATATCAAAGTGAGGTCTAGG+GGG | + | 50569:13789-13808 | None:intergenic | 40.0% | |
| CAAATTACCCATTGTTCCTG+AGG | + | 50569:13433-13452 | None:intergenic | 40.0% | |
| CAAGCTCATGCAACTTTCTA+TGG | - | 50569:13396-13415 | MS.gene050723:CDS | 40.0% | |
| CATATCAAAGTGAGGTCTAG+GGG | + | 50569:13790-13809 | None:intergenic | 40.0% | |
| GACATACCTCCTGTACAAAA+TGG | + | 50569:13862-13881 | None:intergenic | 40.0% | |
| GACATATCAAAGTGAGGTCT+AGG | + | 50569:13792-13811 | None:intergenic | 40.0% | |
| GGTTGAGACATATCAAAGTG+AGG | + | 50569:13798-13817 | None:intergenic | 40.0% | |
| TCAGAGTAGAATCTGCTGTT+TGG | - | 50569:13370-13389 | MS.gene050723:CDS | 40.0% | |
| TTAGCTATAGTCTGAAAGGC+AGG | + | 50569:13819-13838 | None:intergenic | 40.0% | |
| TTTGCACTCCCTAATGACAA+TGG | - | 50569:13750-13769 | MS.gene050723:intron | 40.0% | |
| ! | ATCAGATAGTTTGTGACGCA+AGG | - | 50569:13658-13677 | MS.gene050723:CDS | 40.0% |
| ! | TCATTAGGGAGTGCAAAGTT+TGG | + | 50569:13747-13766 | None:intergenic | 40.0% |
| ! | TTGTTCCCATTTTGTACAGG+AGG | - | 50569:13853-13872 | MS.gene050723:CDS | 40.0% |
| AACTTGTAGGTGGTGCATGT+GGG | - | 50569:13544-13563 | MS.gene050723:CDS | 45.0% | |
| AAGTGATGCCTCAGGAACAA+TGG | - | 50569:13422-13441 | MS.gene050723:CDS | 45.0% | |
| AGTGATGCCTCAGGAACAAT+GGG | - | 50569:13423-13442 | MS.gene050723:CDS | 45.0% | |
| CACCAACCACCATTGTCATT+AGG | + | 50569:13762-13781 | None:intergenic | 45.0% | |
| GCTCATGCAACTTTCTATGG+TGG | - | 50569:13399-13418 | MS.gene050723:CDS | 45.0% | |
| TCCCTAATGACAATGGTGGT+TGG | - | 50569:13757-13776 | MS.gene050723:intron | 45.0% | |
| ! | AATGATGGGAAGTCATGTGG+TGG | - | 50569:13630-13649 | MS.gene050723:CDS | 45.0% |
| ! | TAACTTGTAGGTGGTGCATG+TGG | - | 50569:13543-13562 | MS.gene050723:CDS | 45.0% |
| ! | TTAGGGAGTGCAAAGTTTGG+TGG | + | 50569:13744-13763 | None:intergenic | 45.0% |
| ACGCAAGGCAAGTTCCTCAA+TGG | - | 50569:13673-13692 | MS.gene050723:CDS | 50.0% | |
| GCACTCCCTAATGACAATGG+TGG | - | 50569:13753-13772 | MS.gene050723:intron | 50.0% | |
| GTTCCTCAATGGTGTCTCAG+AGG | - | 50569:13684-13703 | MS.gene050723:CDS | 50.0% | |
| ! | TATGGTGGAAGTGATGCCTC+AGG | - | 50569:13414-13433 | MS.gene050723:CDS | 50.0% |
| GTAGGTGGTGCATGTGGGTA+TGG | - | 50569:13549-13568 | MS.gene050723:CDS | 55.0% | |
| !! | GTGCCTCTGAGACACCATTG+AGG | + | 50569:13690-13709 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 50569 | gene | 13367 | 13893 | 13367 | ID=MS.gene050723 |
| 50569 | mRNA | 13367 | 13893 | 13367 | ID=MS.gene050723.t1;Parent=MS.gene050723 |
| 50569 | exon | 13816 | 13893 | 13816 | ID=MS.gene050723.t1.exon1;Parent=MS.gene050723.t1 |
| 50569 | CDS | 13816 | 13893 | 13816 | ID=cds.MS.gene050723.t1;Parent=MS.gene050723.t1 |
| 50569 | exon | 13367 | 13707 | 13367 | ID=MS.gene050723.t1.exon2;Parent=MS.gene050723.t1 |
| 50569 | CDS | 13367 | 13707 | 13367 | ID=cds.MS.gene050723.t1;Parent=MS.gene050723.t1 |
| Gene Sequence |
| Protein sequence |
