Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050726.t1 | XP_003626098.1 | 99.2 | 133 | 1 | 0 | 1 | 133 | 20 | 152 | 2.10E-74 | 288.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050726.t1 | Q9ZSI1 | 85.3 | 129 | 19 | 0 | 5 | 133 | 25 | 153 | 2.9e-64 | 245.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050726.t1 | G7KVH3 | 99.2 | 133 | 1 | 0 | 1 | 133 | 20 | 152 | 1.5e-74 | 288.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050720 | MS.gene050726 | 0.833867 | 4.17E-56 | -1.69E-46 |
| MS.gene050722 | MS.gene050726 | 0.854851 | 9.35E-62 | -1.69E-46 |
| MS.gene050723 | MS.gene050726 | 0.979315 | 1.06E-147 | -1.69E-46 |
| MS.gene050725 | MS.gene050726 | 0.86561 | 5.21E-65 | -1.69E-46 |
| MS.gene050726 | MS.gene050822 | 0.853588 | 2.16E-61 | -1.69E-46 |
| MS.gene050726 | MS.gene050823 | 0.88723 | 1.71E-72 | -1.69E-46 |
| MS.gene050726 | MS.gene072622 | 0.885126 | 1.06E-71 | -1.69E-46 |
| MS.gene050726 | MS.gene072623 | 0.847597 | 1.05E-59 | -1.69E-46 |
| MS.gene050726 | MS.gene072625 | 0.885757 | 6.17E-72 | -1.69E-46 |
| MS.gene050726 | MS.gene072626 | 0.847973 | 8.24E-60 | -1.69E-46 |
| MS.gene050726 | MS.gene072628 | 0.891658 | 3.25E-74 | -1.69E-46 |
| MS.gene050726 | MS.gene072629 | 0.849897 | 2.41E-60 | -1.69E-46 |
| MS.gene050726 | MS.gene28619 | 0.868906 | 4.61E-66 | -1.69E-46 |
| MS.gene050726 | MS.gene28620 | 0.849848 | 2.48E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATCAAAATTAAGTTATA+TGG | + | 50571:10465-10484 | None:intergenic | 10.0% |
| !! | TAATCAAATTATGATAGTTA+AGG | - | 50571:10439-10458 | MS.gene050726:CDS | 15.0% |
| !!! | CTTAATTTTGATTAACTTGT+AGG | - | 50571:10470-10489 | MS.gene050726:CDS | 20.0% |
| !!! | AATTTTGATTAACTTGTAGG+TGG | - | 50571:10473-10492 | MS.gene050726:CDS | 25.0% |
| AATCTATACACAGATGGATA+TGG | - | 50571:10512-10531 | MS.gene050726:CDS | 30.0% | |
| TATGGAAATCTATACACAGA+TGG | - | 50571:10506-10525 | MS.gene050726:CDS | 30.0% | |
| ACATACCTCCTGTACAAAAT+GGG | + | 50571:10800-10819 | None:intergenic | 35.0% | |
| ACATATCAAAGTGAGGTCTA+GGG | + | 50571:10730-10749 | None:intergenic | 35.0% | |
| AGTACTGCTCTCTTTAATGA+TGG | - | 50571:10554-10573 | MS.gene050726:CDS | 35.0% | |
| ATTTGTGGCAGTAATAGTGA+TGG | + | 50571:10654-10673 | None:intergenic | 35.0% | |
| GTACTGCTCTCTTTAATGAT+GGG | - | 50571:10555-10574 | MS.gene050726:CDS | 35.0% | |
| GTACTTAGCTATAGTCTGAA+AGG | + | 50571:10762-10781 | None:intergenic | 35.0% | |
| ! | GAATTGTTCCCATTTTGTAC+AGG | - | 50571:10789-10808 | MS.gene050726:CDS | 35.0% |
| ! | GTTTGGTGGACAAAAATTTG+TGG | + | 50571:10669-10688 | None:intergenic | 35.0% |
| ! | TTTAATGATGGGAAGTCATG+TGG | - | 50571:10566-10585 | MS.gene050726:CDS | 35.0% |
| ACCAACCACCATTGTCATTA+GGG | + | 50571:10700-10719 | None:intergenic | 40.0% | |
| ACTATAGCTAAGTACAGAGC+TGG | - | 50571:10767-10786 | MS.gene050726:CDS | 40.0% | |
| ATATCAAAGTGAGGTCTAGG+GGG | + | 50571:10728-10747 | None:intergenic | 40.0% | |
| CAAATTACCCATTGTTCCTG+AGG | + | 50571:10370-10389 | None:intergenic | 40.0% | |
| CAAGCTCATGCAACTTTCTA+TGG | - | 50571:10333-10352 | MS.gene050726:CDS | 40.0% | |
| CATATCAAAGTGAGGTCTAG+GGG | + | 50571:10729-10748 | None:intergenic | 40.0% | |
| GACATACCTCCTGTACAAAA+TGG | + | 50571:10801-10820 | None:intergenic | 40.0% | |
| GACATATCAAAGTGAGGTCT+AGG | + | 50571:10731-10750 | None:intergenic | 40.0% | |
| GGTTGAGACATATCAAAGTG+AGG | + | 50571:10737-10756 | None:intergenic | 40.0% | |
| TCAGAGTAGAATCTGCTGTT+TGG | - | 50571:10307-10326 | MS.gene050726:CDS | 40.0% | |
| TTAGCTATAGTCTGAAAGGC+AGG | + | 50571:10758-10777 | None:intergenic | 40.0% | |
| TTTGCACTCCCTAATGACAA+TGG | - | 50571:10689-10708 | MS.gene050726:intron | 40.0% | |
| ! | ATCAGATAGTTTGTGACGCA+AGG | - | 50571:10597-10616 | MS.gene050726:CDS | 40.0% |
| ! | TCATTAGGGAGTGCAAAGTT+TGG | + | 50571:10686-10705 | None:intergenic | 40.0% |
| ! | TTGTTCCCATTTTGTACAGG+AGG | - | 50571:10792-10811 | MS.gene050726:CDS | 40.0% |
| AACTTGTAGGTGGTGCATGT+GGG | - | 50571:10483-10502 | MS.gene050726:CDS | 45.0% | |
| AAGTGATGCCTCAGGAACAA+TGG | - | 50571:10359-10378 | MS.gene050726:CDS | 45.0% | |
| AGTGATGCCTCAGGAACAAT+GGG | - | 50571:10360-10379 | MS.gene050726:CDS | 45.0% | |
| CACCAACCACCATTGTCATT+AGG | + | 50571:10701-10720 | None:intergenic | 45.0% | |
| GCTCATGCAACTTTCTATGG+TGG | - | 50571:10336-10355 | MS.gene050726:CDS | 45.0% | |
| TCCCTAATGACAATGGTGGT+TGG | - | 50571:10696-10715 | MS.gene050726:intron | 45.0% | |
| ! | AATGATGGGAAGTCATGTGG+TGG | - | 50571:10569-10588 | MS.gene050726:CDS | 45.0% |
| ! | TAACTTGTAGGTGGTGCATG+TGG | - | 50571:10482-10501 | MS.gene050726:CDS | 45.0% |
| ! | TTAGGGAGTGCAAAGTTTGG+TGG | + | 50571:10683-10702 | None:intergenic | 45.0% |
| ACGCAAGGCAAGTTCCTCAA+TGG | - | 50571:10612-10631 | MS.gene050726:CDS | 50.0% | |
| GCACTCCCTAATGACAATGG+TGG | - | 50571:10692-10711 | MS.gene050726:intron | 50.0% | |
| GTTCCTCAATGGTGTCTCAG+AGG | - | 50571:10623-10642 | MS.gene050726:CDS | 50.0% | |
| ! | TATGGTGGAAGTGATGCCTC+AGG | - | 50571:10351-10370 | MS.gene050726:CDS | 50.0% |
| GTAGGTGGTGCATGTGGGTA+TGG | - | 50571:10488-10507 | MS.gene050726:CDS | 55.0% | |
| !! | GTGCCTCTGAGACACCATTG+AGG | + | 50571:10629-10648 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 50571 | gene | 10304 | 10832 | 10304 | ID=MS.gene050726 |
| 50571 | mRNA | 10304 | 10832 | 10304 | ID=MS.gene050726.t1;Parent=MS.gene050726 |
| 50571 | exon | 10755 | 10832 | 10755 | ID=MS.gene050726.t1.exon1;Parent=MS.gene050726.t1 |
| 50571 | CDS | 10755 | 10832 | 10755 | ID=cds.MS.gene050726.t1;Parent=MS.gene050726.t1 |
| 50571 | exon | 10304 | 10644 | 10304 | ID=MS.gene050726.t1.exon2;Parent=MS.gene050726.t1 |
| 50571 | CDS | 10304 | 10644 | 10304 | ID=cds.MS.gene050726.t1;Parent=MS.gene050726.t1 |
| Gene Sequence |
| Protein sequence |
