AlfalfaGEDB | MS.gene050822

M. sativa cultivar XinJiangDaYe / MS.gene050822

KEGG: -
GO: GO:0005575 GO:0005576 GO:0008150 GO:0009664 GO:0009987 GO:0016043 GO:0045229 GO:0071554 GO:0071555 GO:0071669 GO:0071840 GO:0110165
PFAM: PF01357.24 PF03330.21

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050822.t1	XP_003626098.1	98.4	254	4	0	1	254	1	254	5.00E-146	526.9

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050822.t1	Q9ZSI1	76.5	251	59	0	4	254	5	255	1.8e-115	416.8

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050822.t1	G7KVH3	98.4	254	4	0	1	254	1	254	3.6e-146	526.9

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050720	MS.gene050822	0.84829	6.74E-60	-1.69E-46
MS.gene050722	MS.gene050822	0.86943	3.11E-66	-1.69E-46
MS.gene050723	MS.gene050822	0.873367	1.55E-67	-1.69E-46
MS.gene050725	MS.gene050822	0.860821	1.58E-63	-1.69E-46
MS.gene050726	MS.gene050822	0.853588	2.16E-61	-1.69E-46
MS.gene050822	MS.gene050823	0.899661	1.59E-77	-1.69E-46
MS.gene050822	MS.gene065424	0.810025	1.41E-50	-1.69E-46
MS.gene050822	MS.gene066602	0.818821	1.60E-52	-1.69E-46
MS.gene050822	MS.gene072622	0.874252	7.81E-68	-1.69E-46
MS.gene050822	MS.gene072623	0.854221	1.42E-61	-1.69E-46

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PPI:

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050822.t1	MTR_7g111010	98.425	254	4	0	1	254	1	254	0.0	521
MS.gene050822.t1	MTR_7g110990	92.913	254	3	1	1	254	1	239	6.85e-175	481
MS.gene050822.t1	MTR_1g085460	85.833	240	34	0	15	254	18	257	2.15e-158	440
MS.gene050822.t1	MTR_1g026020	62.948	251	92	1	4	254	6	255	3.45e-120	343
MS.gene050822.t1	MTR_5g075320	58.871	248	98	3	2	249	4	247	8.51e-107	309
MS.gene050822.t1	MTR_2g097030	59.363	251	96	4	1	248	6	253	1.40e-105	306
MS.gene050822.t1	MTR_3g094060	59.756	246	95	3	3	248	7	248	5.04e-105	305
MS.gene050822.t1	MTR_3g094040	59.756	246	95	3	3	248	7	248	6.00e-105	305
MS.gene050822.t1	MTR_7g061720	63.677	223	77	3	27	248	32	251	8.38e-105	304
MS.gene050822.t1	MTR_5g079950	60.324	247	92	4	5	248	9	252	3.02e-104	303

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050822.t1	AT4G01630	76.494	251	59	0	4	254	5	255	5.78e-148	414
MS.gene050822.t1	AT1G20190	67.857	224	71	1	31	254	30	252	3.87e-112	323
MS.gene050822.t1	AT2G40610	61.224	245	86	3	5	249	14	249	1.26e-107	311
MS.gene050822.t1	AT5G05290	56.967	244	99	3	6	249	14	251	3.52e-106	308
MS.gene050822.t1	AT1G69530	64.865	222	74	3	27	248	27	244	4.93e-103	300
MS.gene050822.t1	AT1G69530	64.865	222	74	3	27	248	27	244	5.92e-103	299
MS.gene050822.t1	AT1G69530	64.865	222	74	3	27	248	27	244	5.92e-103	299
MS.gene050822.t1	AT3G55500	59.677	248	94	5	4	248	9	253	1.02e-102	299
MS.gene050822.t1	AT2G03090	57.787	244	99	3	5	248	8	247	1.75e-102	298
MS.gene050822.t1	AT5G56320	58.000	250	99	4	1	248	1	246	2.87e-102	298

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Find 59 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACATAAGATTTACTATAAA+TGG	0.113150	7.4:+5132716	MS.gene050822:CDS
AACTTGTAGGTGGTGCATGT+GGG	0.650230	7.4:+5132208	MS.gene050822:intron
AAGTGATGCCTCAGGAACAA+TGG	0.569867	7.4:+5132086	MS.gene050822:CDS
AATATACCTCCTATACAAAA+TGG	0.228510	7.4:-5132523	None:intergenic
AATCTATACACAGATGGATA+TGG	0.375722	7.4:+5132237	MS.gene050822:CDS
AATGATGGGAAGTCATGTGG+TGG	0.690106	7.4:+5132294	MS.gene050822:CDS
ACATAAGATTTACTATAAAT+GGG	0.475947	7.4:+5132717	MS.gene050822:CDS
ACATATCAAAGTGAGGTCTA+GGG	0.546299	7.4:-5132452	None:intergenic
ACCAACCACCATTGTCATTA+GGG	0.336869	7.4:-5132422	None:intergenic
ACGCAAGGCAAGTTCCTCAA+TGG	0.572152	7.4:+5132337	MS.gene050822:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATCTATACACAGATGGATA+TGG	+	chr7.4:5132237-5132256	MS.gene050822:CDS	30.0%
TATGGAAATCTATACACAGA+TGG	+	chr7.4:5132231-5132250	MS.gene050822:CDS	30.0%
TGTTGTATTTATGCAGAGTT+GGG	+	chr7.4:5132678-5132697	MS.gene050822:intron	30.0%
TTGTTGTATTTATGCAGAGT+TGG	+	chr7.4:5132677-5132696	MS.gene050822:intron	30.0%
ACATATCAAAGTGAGGTCTA+GGG	-	chr7.4:5132455-5132474	None:intergenic	35.0%
AGTACTGCTCTCTTTAATGA+TGG	+	chr7.4:5132279-5132298	MS.gene050822:CDS	35.0%
ATTTGTGGCAGTAATAGTGA+TGG	-	chr7.4:5132379-5132398	None:intergenic	35.0%
GAACCAATGTCAATGAATTG+GGG	+	chr7.4:5132833-5132852	MS.gene050822:CDS	35.0%
GGAACCAATGTCAATGAATT+GGG	+	chr7.4:5132832-5132851	MS.gene050822:CDS	35.0%
GTACTGCTCTCTTTAATGAT+GGG	+	chr7.4:5132280-5132299	MS.gene050822:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	5131976	5133003	5131976	ID=MS.gene050822
chr7.4	mRNA	5131976	5133003	5131976	ID=MS.gene050822.t1;Parent=MS.gene050822
chr7.4	exon	5131976	5132108	5131976	ID=MS.gene050822.t1.exon1;Parent=MS.gene050822.t1
chr7.4	CDS	5131976	5132108	5131976	ID=cds.MS.gene050822.t1;Parent=MS.gene050822.t1
chr7.4	exon	5132217	5132538	5132217	ID=MS.gene050822.t1.exon2;Parent=MS.gene050822.t1
chr7.4	CDS	5132217	5132538	5132217	ID=cds.MS.gene050822.t1;Parent=MS.gene050822.t1
chr7.4	exon	5132694	5133003	5132694	ID=MS.gene050822.t1.exon3;Parent=MS.gene050822.t1
chr7.4	CDS	5132694	5133003	5132694	ID=cds.MS.gene050822.t1;Parent=MS.gene050822.t1

Gene Sequence

>MS.gene050822

ATGGAAAAATTCATTATCTGTGTTCTTCTATTGTTAATGAATATGGTTACTAAAGAACTCAGAGTAGAATCTGCTGTTTGGCAGCAAGCTCATGCAACTTTCTATGGTGGAAGTGATGCCTCAGGAACAATGGGTAAATTGCTTCTTCTTTTGATTAACTTTATTCAAGTCTTAAGACAAATTAAAGTATTAATCAAATTATGATAGTTAAGCATATAACTTAATTTTGATTAACTTGTAGGTGGTGCATGTGGGTATGGAAATCTATACACAGATGGATATGGTATAAAATCAGCAGCATTGAGTACTGCTCTCTTTAATGATGGGAAGTCATGTGGTGGTTGCTATCAGATAGTTTGTGACGCAAGGCAAGTTCCTCAATGGTGTCTCAGAGGCACTTCCATCACTATTACTGCCACAAATTTTTGTCCACCAAACTTTGCACTCCCTAATGACAATGGTGGTTGGTGTAATCCCCCTAGACCTCACTTTGATATGTCTCAACCTGCCTTTCAGACTATAGCTAAGTACAGAGCTGGAATTGTTCCCATTTTGTATAGGAGGTATATTTTAAATTTCTTCCCTACGACAAAGTCAAATTCTTATCACTGAGCTGACACTTCATAGAAAAACAAATATTTATAGTGATTACACTCCGCTAAATGACAATTAAATCTTAAATATGTATTTGATACAAAGTTTTGTTGTATTTATGCAGAGTTGGGTGCAAAAGAAGTGGAAACATAAGATTTACTATAAATGGGAGAGACTATTTTGAATTGGTGCTTATAAGCAATGTAGGTGGAGGTGGAGAGATTTCTAAAGTTTGGATAAAAGGATCAAAAAAGAATAAATGGGAACCAATGTCAATGAATTGGGGTGCTAATTGGCAAAGCCTAAGCTATCTCAATGGTCAAAGTTTATCATTTAGAATTCAACTCAAAAATGGGAAGACTCGCACAGCTATCAATGTTGCACCTTCCAATTGGAGATTTGGCCAGTCTTACAAAAGTAACGTTCAGTTCTGA

Protein sequence

>MS.gene050822.t1

MEKFIICVLLLLMNMVTKELRVESAVWQQAHATFYGGSDASGTMGGACGYGNLYTDGYGIKSAALSTALFNDGKSCGGCYQIVCDARQVPQWCLRGTSITITATNFCPPNFALPNDNGGWCNPPRPHFDMSQPAFQTIAKYRAGIVPILYRRVGCKRSGNIRFTINGRDYFELVLISNVGGGGEISKVWIKGSKKNKWEPMSMNWGANWQSLSYLNGQSLSFRIQLKNGKTRTAINVAPSNWRFGQSYKSNVQF