Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050822.t1 | XP_003626098.1 | 98.4 | 254 | 4 | 0 | 1 | 254 | 1 | 254 | 5.00E-146 | 526.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050822.t1 | Q9ZSI1 | 76.5 | 251 | 59 | 0 | 4 | 254 | 5 | 255 | 1.8e-115 | 416.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050822.t1 | G7KVH3 | 98.4 | 254 | 4 | 0 | 1 | 254 | 1 | 254 | 3.6e-146 | 526.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050720 | MS.gene050822 | 0.84829 | 6.74E-60 | -1.69E-46 |
| MS.gene050722 | MS.gene050822 | 0.86943 | 3.11E-66 | -1.69E-46 |
| MS.gene050723 | MS.gene050822 | 0.873367 | 1.55E-67 | -1.69E-46 |
| MS.gene050725 | MS.gene050822 | 0.860821 | 1.58E-63 | -1.69E-46 |
| MS.gene050726 | MS.gene050822 | 0.853588 | 2.16E-61 | -1.69E-46 |
| MS.gene050822 | MS.gene050823 | 0.899661 | 1.59E-77 | -1.69E-46 |
| MS.gene050822 | MS.gene065424 | 0.810025 | 1.41E-50 | -1.69E-46 |
| MS.gene050822 | MS.gene066602 | 0.818821 | 1.60E-52 | -1.69E-46 |
| MS.gene050822 | MS.gene072622 | 0.874252 | 7.81E-68 | -1.69E-46 |
| MS.gene050822 | MS.gene072623 | 0.854221 | 1.42E-61 | -1.69E-46 |
| MS.gene050822 | MS.gene072625 | 0.871046 | 9.21E-67 | -1.69E-46 |
| MS.gene050822 | MS.gene072626 | 0.852728 | 3.81E-61 | -1.69E-46 |
| MS.gene050822 | MS.gene072628 | 0.873911 | 1.02E-67 | -1.69E-46 |
| MS.gene050822 | MS.gene072629 | 0.853773 | 1.91E-61 | -1.69E-46 |
| MS.gene050822 | MS.gene23436 | 0.80377 | 2.98E-49 | -1.69E-46 |
| MS.gene050822 | MS.gene28619 | 0.956267 | 4.41E-114 | -1.69E-46 |
| MS.gene050822 | MS.gene28620 | 0.829803 | 4.20E-55 | -1.69E-46 |
| MS.gene050822 | MS.gene45893 | 0.815295 | 9.95E-52 | -1.69E-46 |
| MS.gene050822 | MS.gene48885 | 0.806538 | 7.84E-50 | -1.69E-46 |
| MS.gene050822 | MS.gene50279 | 0.80235 | 5.85E-49 | -1.69E-46 |
| MS.gene050822 | MS.gene55078 | 0.826003 | 3.44E-54 | -1.69E-46 |
| MS.gene050822 | MS.gene56381 | 0.811502 | 6.78E-51 | -1.69E-46 |
| MS.gene050822 | MS.gene60913 | 0.805532 | 1.28E-49 | -1.69E-46 |
| MS.gene050822 | MS.gene71350 | 0.805456 | 1.32E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050822.t1 | MTR_7g111010 | 98.425 | 254 | 4 | 0 | 1 | 254 | 1 | 254 | 0.0 | 521 |
| MS.gene050822.t1 | MTR_7g110990 | 92.913 | 254 | 3 | 1 | 1 | 254 | 1 | 239 | 6.85e-175 | 481 |
| MS.gene050822.t1 | MTR_1g085460 | 85.833 | 240 | 34 | 0 | 15 | 254 | 18 | 257 | 2.15e-158 | 440 |
| MS.gene050822.t1 | MTR_1g026020 | 62.948 | 251 | 92 | 1 | 4 | 254 | 6 | 255 | 3.45e-120 | 343 |
| MS.gene050822.t1 | MTR_5g075320 | 58.871 | 248 | 98 | 3 | 2 | 249 | 4 | 247 | 8.51e-107 | 309 |
| MS.gene050822.t1 | MTR_2g097030 | 59.363 | 251 | 96 | 4 | 1 | 248 | 6 | 253 | 1.40e-105 | 306 |
| MS.gene050822.t1 | MTR_3g094060 | 59.756 | 246 | 95 | 3 | 3 | 248 | 7 | 248 | 5.04e-105 | 305 |
| MS.gene050822.t1 | MTR_3g094040 | 59.756 | 246 | 95 | 3 | 3 | 248 | 7 | 248 | 6.00e-105 | 305 |
| MS.gene050822.t1 | MTR_7g061720 | 63.677 | 223 | 77 | 3 | 27 | 248 | 32 | 251 | 8.38e-105 | 304 |
| MS.gene050822.t1 | MTR_5g079950 | 60.324 | 247 | 92 | 4 | 5 | 248 | 9 | 252 | 3.02e-104 | 303 |
| MS.gene050822.t1 | MTR_5g041700 | 64.912 | 228 | 75 | 4 | 27 | 254 | 25 | 247 | 3.70e-104 | 302 |
| MS.gene050822.t1 | MTR_4g081950 | 61.224 | 245 | 90 | 4 | 4 | 248 | 39 | 278 | 4.53e-104 | 303 |
| MS.gene050822.t1 | MTR_3g435430 | 64.474 | 228 | 76 | 4 | 27 | 254 | 25 | 247 | 6.05e-104 | 301 |
| MS.gene050822.t1 | MTR_3g466790 | 59.592 | 245 | 95 | 3 | 5 | 249 | 10 | 250 | 5.45e-102 | 297 |
| MS.gene050822.t1 | MTR_7g011060 | 61.224 | 245 | 82 | 5 | 5 | 248 | 3 | 235 | 6.77e-102 | 296 |
| MS.gene050822.t1 | MTR_3g466760 | 59.184 | 245 | 96 | 3 | 5 | 249 | 16 | 256 | 3.85e-100 | 293 |
| MS.gene050822.t1 | MTR_4g063975 | 56.126 | 253 | 103 | 4 | 1 | 248 | 2 | 251 | 8.67e-98 | 286 |
| MS.gene050822.t1 | MTR_6g012520 | 64.414 | 222 | 67 | 4 | 27 | 248 | 28 | 237 | 1.08e-91 | 270 |
| MS.gene050822.t1 | MTR_5g011210 | 51.550 | 258 | 113 | 6 | 4 | 254 | 10 | 262 | 4.04e-87 | 259 |
| MS.gene050822.t1 | MTR_4g102450 | 51.373 | 255 | 116 | 4 | 5 | 254 | 10 | 261 | 5.60e-87 | 259 |
| MS.gene050822.t1 | MTR_7g013120 | 48.988 | 247 | 118 | 4 | 9 | 254 | 14 | 253 | 4.80e-85 | 254 |
| MS.gene050822.t1 | MTR_7g013150 | 50.199 | 251 | 116 | 5 | 5 | 254 | 11 | 253 | 9.13e-85 | 253 |
| MS.gene050822.t1 | MTR_7g013140 | 51.417 | 247 | 114 | 4 | 9 | 254 | 10 | 251 | 1.05e-84 | 253 |
| MS.gene050822.t1 | MTR_4g124340 | 50.673 | 223 | 105 | 3 | 27 | 248 | 61 | 279 | 7.08e-84 | 252 |
| MS.gene050822.t1 | MTR_8g464350 | 50.000 | 248 | 115 | 5 | 8 | 254 | 15 | 254 | 1.07e-81 | 245 |
| MS.gene050822.t1 | MTR_8g058990 | 50.218 | 229 | 108 | 4 | 27 | 254 | 30 | 253 | 1.11e-77 | 235 |
| MS.gene050822.t1 | MTR_0040s0140 | 47.773 | 247 | 121 | 4 | 4 | 249 | 13 | 252 | 4.44e-77 | 234 |
| MS.gene050822.t1 | MTR_2g090255 | 46.531 | 245 | 127 | 3 | 6 | 249 | 7 | 248 | 2.09e-74 | 227 |
| MS.gene050822.t1 | MTR_5g076950 | 48.444 | 225 | 109 | 3 | 27 | 249 | 41 | 260 | 1.38e-71 | 220 |
| MS.gene050822.t1 | MTR_3g064510 | 46.725 | 229 | 115 | 5 | 27 | 249 | 53 | 280 | 1.12e-67 | 211 |
| MS.gene050822.t1 | MTR_6g012500 | 58.333 | 96 | 39 | 1 | 153 | 248 | 181 | 275 | 2.88e-32 | 119 |
| MS.gene050822.t1 | MTR_0008s0120 | 31.579 | 247 | 108 | 7 | 8 | 254 | 14 | 199 | 1.94e-31 | 115 |
| MS.gene050822.t1 | MTR_2g090270 | 30.962 | 239 | 139 | 8 | 27 | 254 | 35 | 258 | 3.77e-30 | 113 |
| MS.gene050822.t1 | MTR_2g090265 | 30.153 | 262 | 157 | 9 | 4 | 254 | 10 | 256 | 2.19e-29 | 111 |
| MS.gene050822.t1 | MTR_0122s0020 | 31.381 | 239 | 138 | 9 | 27 | 254 | 41 | 264 | 3.88e-26 | 103 |
| MS.gene050822.t1 | MTR_1g085365 | 81.818 | 55 | 10 | 0 | 198 | 252 | 4 | 58 | 7.12e-26 | 96.7 |
| MS.gene050822.t1 | MTR_0118s0070 | 32.780 | 241 | 126 | 10 | 32 | 254 | 53 | 275 | 1.32e-25 | 102 |
| MS.gene050822.t1 | MTR_4g028640 | 31.765 | 255 | 135 | 12 | 17 | 254 | 32 | 264 | 2.19e-24 | 98.6 |
| MS.gene050822.t1 | MTR_4g099370 | 28.077 | 260 | 157 | 11 | 5 | 254 | 11 | 250 | 3.27e-22 | 92.4 |
| MS.gene050822.t1 | MTR_0809s0010 | 30.041 | 243 | 128 | 12 | 27 | 250 | 47 | 266 | 2.11e-21 | 90.5 |
| MS.gene050822.t1 | MTR_5g013440 | 27.132 | 258 | 162 | 11 | 5 | 254 | 12 | 251 | 3.05e-20 | 87.0 |
| MS.gene050822.t1 | MTR_4g075790 | 31.224 | 237 | 136 | 11 | 28 | 254 | 24 | 243 | 2.14e-18 | 82.4 |
| MS.gene050822.t1 | MTR_4g098930 | 27.925 | 265 | 165 | 11 | 1 | 253 | 1 | 251 | 1.11e-16 | 77.4 |
| MS.gene050822.t1 | MTR_0122s0020 | 31.818 | 176 | 94 | 9 | 27 | 191 | 41 | 201 | 4.40e-14 | 69.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050822.t1 | AT4G01630 | 76.494 | 251 | 59 | 0 | 4 | 254 | 5 | 255 | 5.78e-148 | 414 |
| MS.gene050822.t1 | AT1G20190 | 67.857 | 224 | 71 | 1 | 31 | 254 | 30 | 252 | 3.87e-112 | 323 |
| MS.gene050822.t1 | AT2G40610 | 61.224 | 245 | 86 | 3 | 5 | 249 | 14 | 249 | 1.26e-107 | 311 |
| MS.gene050822.t1 | AT5G05290 | 56.967 | 244 | 99 | 3 | 6 | 249 | 14 | 251 | 3.52e-106 | 308 |
| MS.gene050822.t1 | AT1G69530 | 64.865 | 222 | 74 | 3 | 27 | 248 | 27 | 244 | 4.93e-103 | 300 |
| MS.gene050822.t1 | AT1G69530 | 64.865 | 222 | 74 | 3 | 27 | 248 | 27 | 244 | 5.92e-103 | 299 |
| MS.gene050822.t1 | AT1G69530 | 64.865 | 222 | 74 | 3 | 27 | 248 | 27 | 244 | 5.92e-103 | 299 |
| MS.gene050822.t1 | AT3G55500 | 59.677 | 248 | 94 | 5 | 4 | 248 | 9 | 253 | 1.02e-102 | 299 |
| MS.gene050822.t1 | AT2G03090 | 57.787 | 244 | 99 | 3 | 5 | 248 | 8 | 247 | 1.75e-102 | 298 |
| MS.gene050822.t1 | AT5G56320 | 58.000 | 250 | 99 | 4 | 1 | 248 | 1 | 246 | 2.87e-102 | 298 |
| MS.gene050822.t1 | AT5G56320 | 58.000 | 250 | 99 | 4 | 1 | 248 | 1 | 246 | 2.87e-102 | 298 |
| MS.gene050822.t1 | AT5G56320 | 58.000 | 250 | 99 | 4 | 1 | 248 | 1 | 246 | 1.67e-101 | 298 |
| MS.gene050822.t1 | AT2G39700 | 62.946 | 224 | 79 | 3 | 26 | 248 | 30 | 250 | 1.97e-101 | 296 |
| MS.gene050822.t1 | AT5G02260 | 56.574 | 251 | 101 | 4 | 3 | 248 | 4 | 251 | 6.78e-101 | 295 |
| MS.gene050822.t1 | AT2G28950 | 57.312 | 253 | 98 | 5 | 1 | 248 | 3 | 250 | 7.00e-101 | 294 |
| MS.gene050822.t1 | AT2G37640 | 62.500 | 224 | 80 | 3 | 27 | 249 | 36 | 256 | 1.78e-100 | 293 |
| MS.gene050822.t1 | AT3G29030 | 60.360 | 222 | 77 | 3 | 27 | 248 | 38 | 248 | 1.91e-97 | 286 |
| MS.gene050822.t1 | AT1G26770 | 58.921 | 241 | 95 | 3 | 8 | 248 | 7 | 243 | 1.05e-94 | 278 |
| MS.gene050822.t1 | AT1G26770 | 58.921 | 241 | 95 | 3 | 8 | 248 | 17 | 253 | 1.45e-94 | 278 |
| MS.gene050822.t1 | AT1G20190 | 65.285 | 193 | 66 | 1 | 62 | 254 | 2 | 193 | 2.13e-91 | 268 |
| MS.gene050822.t1 | AT3G15370 | 52.823 | 248 | 108 | 3 | 4 | 250 | 7 | 246 | 1.01e-90 | 268 |
| MS.gene050822.t1 | AT1G69530 | 63.547 | 203 | 68 | 4 | 27 | 229 | 27 | 223 | 5.30e-89 | 264 |
| MS.gene050822.t1 | AT1G69530 | 65.641 | 195 | 61 | 4 | 27 | 221 | 27 | 215 | 1.63e-88 | 261 |
| MS.gene050822.t1 | AT2G37640 | 59.420 | 207 | 80 | 3 | 44 | 249 | 28 | 231 | 5.97e-87 | 258 |
| MS.gene050822.t1 | AT1G12560 | 54.585 | 229 | 99 | 4 | 27 | 254 | 37 | 261 | 1.42e-85 | 256 |
| MS.gene050822.t1 | AT3G15370 | 47.122 | 278 | 108 | 4 | 4 | 250 | 7 | 276 | 5.48e-84 | 252 |
| MS.gene050822.t1 | AT1G62980 | 49.200 | 250 | 120 | 6 | 8 | 254 | 11 | 256 | 5.26e-81 | 244 |
| MS.gene050822.t1 | AT5G39270 | 51.754 | 228 | 104 | 4 | 27 | 254 | 58 | 279 | 6.96e-81 | 244 |
| MS.gene050822.t1 | AT5G39300 | 50.439 | 228 | 107 | 4 | 27 | 254 | 55 | 276 | 2.40e-79 | 240 |
| MS.gene050822.t1 | AT5G39290 | 51.316 | 228 | 105 | 4 | 27 | 254 | 58 | 279 | 2.58e-79 | 240 |
| MS.gene050822.t1 | AT5G39310 | 49.787 | 235 | 111 | 5 | 21 | 254 | 84 | 312 | 3.69e-79 | 241 |
| MS.gene050822.t1 | AT5G39280 | 50.000 | 228 | 108 | 4 | 27 | 254 | 54 | 275 | 7.54e-79 | 239 |
| MS.gene050822.t1 | AT4G38210 | 44.444 | 252 | 131 | 5 | 1 | 249 | 5 | 250 | 6.79e-70 | 216 |
| MS.gene050822.t1 | AT3G03220 | 45.333 | 225 | 116 | 3 | 27 | 249 | 40 | 259 | 1.46e-68 | 213 |
| MS.gene050822.t1 | AT5G39260 | 45.852 | 229 | 112 | 5 | 27 | 254 | 45 | 262 | 2.35e-62 | 196 |
| MS.gene050822.t1 | AT4G28250 | 30.189 | 265 | 154 | 9 | 6 | 254 | 11 | 260 | 6.02e-32 | 118 |
| MS.gene050822.t1 | AT2G20750 | 31.970 | 269 | 151 | 9 | 6 | 254 | 11 | 267 | 2.18e-31 | 117 |
| MS.gene050822.t1 | AT1G65680 | 34.711 | 242 | 129 | 11 | 27 | 254 | 47 | 273 | 1.44e-29 | 112 |
| MS.gene050822.t1 | AT4G28250 | 29.167 | 264 | 152 | 9 | 6 | 254 | 11 | 254 | 8.27e-29 | 110 |
| MS.gene050822.t1 | AT2G45110 | 34.156 | 243 | 123 | 11 | 30 | 254 | 36 | 259 | 1.33e-24 | 99.0 |
| MS.gene050822.t1 | AT1G65681 | 31.004 | 229 | 125 | 11 | 41 | 254 | 12 | 222 | 3.61e-21 | 89.0 |
| MS.gene050822.t1 | AT2G20750 | 31.707 | 205 | 109 | 8 | 6 | 191 | 11 | 203 | 1.03e-19 | 85.1 |
| MS.gene050822.t1 | AT4G28250 | 28.293 | 205 | 113 | 8 | 6 | 196 | 11 | 195 | 6.15e-18 | 80.1 |
| MS.gene050822.t1 | AT4G17030 | 25.483 | 259 | 174 | 8 | 1 | 252 | 1 | 247 | 3.03e-17 | 79.0 |
| MS.gene050822.t1 | AT4G28250 | 28.500 | 200 | 109 | 8 | 6 | 191 | 11 | 190 | 3.26e-17 | 78.2 |
| MS.gene050822.t1 | AT3G60570 | 29.098 | 244 | 137 | 10 | 27 | 254 | 34 | 257 | 9.40e-16 | 75.1 |
| MS.gene050822.t1 | AT4G38400 | 27.354 | 223 | 123 | 9 | 50 | 254 | 47 | 248 | 7.77e-15 | 72.4 |
| MS.gene050822.t1 | AT3G45970 | 28.696 | 230 | 122 | 11 | 45 | 254 | 14 | 221 | 2.54e-14 | 70.9 |
| MS.gene050822.t1 | AT3G45960 | 27.778 | 216 | 131 | 10 | 50 | 254 | 46 | 247 | 1.12e-12 | 66.6 |
| MS.gene050822.t1 | AT3G45970 | 28.000 | 225 | 120 | 11 | 50 | 254 | 46 | 248 | 1.03e-11 | 63.9 |
| MS.gene050822.t1 | AT3G60570 | 28.319 | 226 | 126 | 10 | 45 | 254 | 4 | 209 | 1.66e-11 | 62.4 |
Find 59 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACATAAGATTTACTATAAA+TGG | 0.113150 | 7.4:+5132716 | MS.gene050822:CDS |
| ATTTCTAAAGTTTGGATAAA+AGG | 0.191747 | 7.4:+5132791 | MS.gene050822:CDS |
| AATATACCTCCTATACAAAA+TGG | 0.228510 | 7.4:-5132523 | None:intergenic |
| GTGGAGAGATTTCTAAAGTT+TGG | 0.231344 | 7.4:+5132783 | MS.gene050822:CDS |
| TCTTCTATTGTTAATGAATA+TGG | 0.235887 | 7.4:+5131999 | MS.gene050822:CDS |
| GCACCTTCCAATTGGAGATT+TGG | 0.278944 | 7.4:+5132950 | MS.gene050822:CDS |
| CAAGCTCATGCAACTTTCTA+TGG | 0.321631 | 7.4:+5132060 | MS.gene050822:CDS |
| GGGAACCAATGTCAATGAAT+TGG | 0.333852 | 7.4:+5132831 | MS.gene050822:CDS |
| ACCAACCACCATTGTCATTA+GGG | 0.336869 | 7.4:-5132422 | None:intergenic |
| CAATGAATTGGGGTGCTAAT+TGG | 0.343861 | 7.4:+5132843 | MS.gene050822:CDS |
| CACCAACCACCATTGTCATT+AGG | 0.351626 | 7.4:-5132423 | None:intergenic |
| AATCTATACACAGATGGATA+TGG | 0.375722 | 7.4:+5132237 | MS.gene050822:CDS |
| GGAACCAATGTCAATGAATT+GGG | 0.404942 | 7.4:+5132832 | MS.gene050822:CDS |
| AGTACTGCTCTCTTTAATGA+TGG | 0.424107 | 7.4:+5132279 | MS.gene050822:CDS |
| TCATTAGGGAGTGCAAAGTT+TGG | 0.443320 | 7.4:-5132408 | None:intergenic |
| ATATACCTCCTATACAAAAT+GGG | 0.446349 | 7.4:-5132522 | None:intergenic |
| TCAGAGTAGAATCTGCTGTT+TGG | 0.451820 | 7.4:+5132034 | MS.gene050822:CDS |
| TCAATGTTGCACCTTCCAAT+TGG | 0.451937 | 7.4:+5132942 | MS.gene050822:CDS |
| GACATATCAAAGTGAGGTCT+AGG | 0.457100 | 7.4:-5132453 | None:intergenic |
| ACATAAGATTTACTATAAAT+GGG | 0.475947 | 7.4:+5132717 | MS.gene050822:CDS |
| TTTGACCATTGAGATAGCTT+AGG | 0.487665 | 7.4:-5132871 | None:intergenic |
| AGTGATGCCTCAGGAACAAT+GGG | 0.514794 | 7.4:+5132087 | MS.gene050822:CDS |
| AGACTGGCCAAATCTCCAAT+TGG | 0.514918 | 7.4:-5132957 | None:intergenic |
| TTAGGGAGTGCAAAGTTTGG+TGG | 0.522538 | 7.4:-5132405 | None:intergenic |
| GTAGGTGGTGCATGTGGGTA+TGG | 0.533831 | 7.4:+5132213 | MS.gene050822:intron |
| GCACCCCAATTCATTGACAT+TGG | 0.537043 | 7.4:-5132836 | None:intergenic |
| ATAAGCAATGTAGGTGGAGG+TGG | 0.538451 | 7.4:+5132764 | MS.gene050822:CDS |
| CAATTTACCCATTGTTCCTG+AGG | 0.543572 | 7.4:-5132094 | None:intergenic |
| GTACTTAGCTATAGTCTGAA+AGG | 0.544966 | 7.4:-5132484 | None:intergenic |
| ACATATCAAAGTGAGGTCTA+GGG | 0.546299 | 7.4:-5132452 | None:intergenic |
| TTAGCTATAGTCTGAAAGGC+AGG | 0.550027 | 7.4:-5132480 | None:intergenic |
| ATCAGATAGTTTGTGACGCA+AGG | 0.552577 | 7.4:+5132322 | MS.gene050822:CDS |
| ACTATAGCTAAGTACAGAGC+TGG | 0.557662 | 7.4:+5132492 | MS.gene050822:CDS |
| GTACTGCTCTCTTTAATGAT+GGG | 0.561983 | 7.4:+5132280 | MS.gene050822:CDS |
| TAACTTGTAGGTGGTGCATG+TGG | 0.565894 | 7.4:+5132207 | MS.gene050822:intron |
| AAGTGATGCCTCAGGAACAA+TGG | 0.569867 | 7.4:+5132086 | MS.gene050822:CDS |
| TATGGTGGAAGTGATGCCTC+AGG | 0.571406 | 7.4:+5132078 | MS.gene050822:CDS |
| ACGCAAGGCAAGTTCCTCAA+TGG | 0.572152 | 7.4:+5132337 | MS.gene050822:CDS |
| ATTTGTGGCAGTAATAGTGA+TGG | 0.572348 | 7.4:-5132376 | None:intergenic |
| TATGGAAATCTATACACAGA+TGG | 0.589533 | 7.4:+5132231 | MS.gene050822:CDS |
| CTTATAAGCAATGTAGGTGG+AGG | 0.589768 | 7.4:+5132761 | MS.gene050822:CDS |
| TTGGTGCTTATAAGCAATGT+AGG | 0.593341 | 7.4:+5132755 | MS.gene050822:CDS |
| CATATCAAAGTGAGGTCTAG+GGG | 0.597169 | 7.4:-5132451 | None:intergenic |
| CAAAGCCTAAGCTATCTCAA+TGG | 0.606300 | 7.4:+5132866 | MS.gene050822:CDS |
| TGGCCAAATCTCCAATTGGA+AGG | 0.609473 | 7.4:-5132953 | None:intergenic |
| GCTCATGCAACTTTCTATGG+TGG | 0.611814 | 7.4:+5132063 | MS.gene050822:CDS |
| TCCCTAATGACAATGGTGGT+TGG | 0.616216 | 7.4:+5132421 | MS.gene050822:CDS |
| AGAGTTGGGTGCAAAAGAAG+TGG | 0.645968 | 7.4:+5132692 | MS.gene050822:intron |
| AACTTGTAGGTGGTGCATGT+GGG | 0.650230 | 7.4:+5132208 | MS.gene050822:intron |
| GTTCCTCAATGGTGTCTCAG+AGG | 0.656491 | 7.4:+5132348 | MS.gene050822:CDS |
| GTGCTTATAAGCAATGTAGG+TGG | 0.665110 | 7.4:+5132758 | MS.gene050822:CDS |
| TTTGCACTCCCTAATGACAA+TGG | 0.665294 | 7.4:+5132414 | MS.gene050822:CDS |
| TTTAATGATGGGAAGTCATG+TGG | 0.667500 | 7.4:+5132291 | MS.gene050822:CDS |
| GCACTCCCTAATGACAATGG+TGG | 0.672667 | 7.4:+5132417 | MS.gene050822:CDS |
| GTGCCTCTGAGACACCATTG+AGG | 0.683828 | 7.4:-5132351 | None:intergenic |
| AATGATGGGAAGTCATGTGG+TGG | 0.690106 | 7.4:+5132294 | MS.gene050822:CDS |
| ATATCAAAGTGAGGTCTAGG+GGG | 0.691110 | 7.4:-5132450 | None:intergenic |
| GGTTGAGACATATCAAAGTG+AGG | 0.694334 | 7.4:-5132459 | None:intergenic |
| GAACCAATGTCAATGAATTG+GGG | 0.713611 | 7.4:+5132833 | MS.gene050822:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACATAAGATTTACTATAAA+TGG | + | chr7.4:5132716-5132735 | MS.gene050822:CDS | 15.0% |
| !! | ACATAAGATTTACTATAAAT+GGG | + | chr7.4:5132717-5132736 | MS.gene050822:CDS | 15.0% |
| !! | AAGGATCAAAAAAGAATAAA+TGG | + | chr7.4:5132810-5132829 | MS.gene050822:CDS | 20.0% |
| !! | AGGATCAAAAAAGAATAAAT+GGG | + | chr7.4:5132811-5132830 | MS.gene050822:CDS | 20.0% |
| !! | TCTTCTATTGTTAATGAATA+TGG | + | chr7.4:5131999-5132018 | MS.gene050822:CDS | 20.0% |
| !! | TTAGAATTCAACTCAAAAAT+GGG | + | chr7.4:5132903-5132922 | MS.gene050822:CDS | 20.0% |
| !!! | AAGATTTAATTGTCATTTAG+CGG | - | chr7.4:5132634-5132653 | None:intergenic | 20.0% |
| !!! | ATTTCTAAAGTTTGGATAAA+AGG | + | chr7.4:5132791-5132810 | MS.gene050822:CDS | 20.0% |
| !!! | CTTAATTTTGATTAACTTGT+AGG | + | chr7.4:5132195-5132214 | MS.gene050822:intron | 20.0% |
| !!! | TTTAGAATTCAACTCAAAAA+TGG | + | chr7.4:5132902-5132921 | MS.gene050822:CDS | 20.0% |
| ! | AATATACCTCCTATACAAAA+TGG | - | chr7.4:5132526-5132545 | None:intergenic | 25.0% |
| ! | ATATACCTCCTATACAAAAT+GGG | - | chr7.4:5132525-5132544 | None:intergenic | 25.0% |
| !!! | AATTTTGATTAACTTGTAGG+TGG | + | chr7.4:5132198-5132217 | MS.gene050822:intron | 25.0% |
| AATCTATACACAGATGGATA+TGG | + | chr7.4:5132237-5132256 | MS.gene050822:CDS | 30.0% | |
| TATGGAAATCTATACACAGA+TGG | + | chr7.4:5132231-5132250 | MS.gene050822:CDS | 30.0% | |
| TGTTGTATTTATGCAGAGTT+GGG | + | chr7.4:5132678-5132697 | MS.gene050822:intron | 30.0% | |
| TTGTTGTATTTATGCAGAGT+TGG | + | chr7.4:5132677-5132696 | MS.gene050822:intron | 30.0% | |
| ! | AAGAATTTGACTTTGTCGTA+GGG | - | chr7.4:5132560-5132579 | None:intergenic | 30.0% |
| ! | GAATTGTTCCCATTTTGTAT+AGG | + | chr7.4:5132514-5132533 | MS.gene050822:CDS | 30.0% |
| ! | TAAGAATTTGACTTTGTCGT+AGG | - | chr7.4:5132561-5132580 | None:intergenic | 30.0% |
| ACATATCAAAGTGAGGTCTA+GGG | - | chr7.4:5132455-5132474 | None:intergenic | 35.0% | |
| AGTACTGCTCTCTTTAATGA+TGG | + | chr7.4:5132279-5132298 | MS.gene050822:CDS | 35.0% | |
| ATTTGTGGCAGTAATAGTGA+TGG | - | chr7.4:5132379-5132398 | None:intergenic | 35.0% | |
| GAACCAATGTCAATGAATTG+GGG | + | chr7.4:5132833-5132852 | MS.gene050822:CDS | 35.0% | |
| GGAACCAATGTCAATGAATT+GGG | + | chr7.4:5132832-5132851 | MS.gene050822:CDS | 35.0% | |
| GTACTGCTCTCTTTAATGAT+GGG | + | chr7.4:5132280-5132299 | MS.gene050822:CDS | 35.0% | |
| GTACTTAGCTATAGTCTGAA+AGG | - | chr7.4:5132487-5132506 | None:intergenic | 35.0% | |
| TTTGACCATTGAGATAGCTT+AGG | - | chr7.4:5132874-5132893 | None:intergenic | 35.0% | |
| ! | GTTTGGTGGACAAAAATTTG+TGG | - | chr7.4:5132394-5132413 | None:intergenic | 35.0% |
| ! | TTGGTGCTTATAAGCAATGT+AGG | + | chr7.4:5132755-5132774 | MS.gene050822:CDS | 35.0% |
| ! | TTGTTCCCATTTTGTATAGG+AGG | + | chr7.4:5132517-5132536 | MS.gene050822:CDS | 35.0% |
| ! | TTTAATGATGGGAAGTCATG+TGG | + | chr7.4:5132291-5132310 | MS.gene050822:CDS | 35.0% |
| !! | GTGGAGAGATTTCTAAAGTT+TGG | + | chr7.4:5132783-5132802 | MS.gene050822:CDS | 35.0% |
| !! | TGGGAGAGACTATTTTGAAT+TGG | + | chr7.4:5132736-5132755 | MS.gene050822:CDS | 35.0% |
| !!! | GAACGTTACTTTTGTAAGAC+TGG | - | chr7.4:5132976-5132995 | None:intergenic | 35.0% |
| ACCAACCACCATTGTCATTA+GGG | - | chr7.4:5132425-5132444 | None:intergenic | 40.0% | |
| ACTATAGCTAAGTACAGAGC+TGG | + | chr7.4:5132492-5132511 | MS.gene050822:CDS | 40.0% | |
| ATATCAAAGTGAGGTCTAGG+GGG | - | chr7.4:5132453-5132472 | None:intergenic | 40.0% | |
| CAAAGCCTAAGCTATCTCAA+TGG | + | chr7.4:5132866-5132885 | MS.gene050822:CDS | 40.0% | |
| CAAGCTCATGCAACTTTCTA+TGG | + | chr7.4:5132060-5132079 | MS.gene050822:CDS | 40.0% | |
| CAATTTACCCATTGTTCCTG+AGG | - | chr7.4:5132097-5132116 | None:intergenic | 40.0% | |
| CATATCAAAGTGAGGTCTAG+GGG | - | chr7.4:5132454-5132473 | None:intergenic | 40.0% | |
| CTTATAAGCAATGTAGGTGG+AGG | + | chr7.4:5132761-5132780 | MS.gene050822:CDS | 40.0% | |
| GACATATCAAAGTGAGGTCT+AGG | - | chr7.4:5132456-5132475 | None:intergenic | 40.0% | |
| GGGAACCAATGTCAATGAAT+TGG | + | chr7.4:5132831-5132850 | MS.gene050822:CDS | 40.0% | |
| GGTTGAGACATATCAAAGTG+AGG | - | chr7.4:5132462-5132481 | None:intergenic | 40.0% | |
| GTGCTTATAAGCAATGTAGG+TGG | + | chr7.4:5132758-5132777 | MS.gene050822:CDS | 40.0% | |
| TCAATGTTGCACCTTCCAAT+TGG | + | chr7.4:5132942-5132961 | MS.gene050822:CDS | 40.0% | |
| TCAGAGTAGAATCTGCTGTT+TGG | + | chr7.4:5132034-5132053 | MS.gene050822:CDS | 40.0% | |
| TTAGCTATAGTCTGAAAGGC+AGG | - | chr7.4:5132483-5132502 | None:intergenic | 40.0% | |
| TTTGCACTCCCTAATGACAA+TGG | + | chr7.4:5132414-5132433 | MS.gene050822:CDS | 40.0% | |
| ! | ATCAGATAGTTTGTGACGCA+AGG | + | chr7.4:5132322-5132341 | MS.gene050822:CDS | 40.0% |
| ! | CAATGAATTGGGGTGCTAAT+TGG | + | chr7.4:5132843-5132862 | MS.gene050822:CDS | 40.0% |
| ! | TCATTAGGGAGTGCAAAGTT+TGG | - | chr7.4:5132411-5132430 | None:intergenic | 40.0% |
| AACTTGTAGGTGGTGCATGT+GGG | + | chr7.4:5132208-5132227 | MS.gene050822:intron | 45.0% | |
| AAGTGATGCCTCAGGAACAA+TGG | + | chr7.4:5132086-5132105 | MS.gene050822:CDS | 45.0% | |
| AGAGTTGGGTGCAAAAGAAG+TGG | + | chr7.4:5132692-5132711 | MS.gene050822:intron | 45.0% | |
| AGTGATGCCTCAGGAACAAT+GGG | + | chr7.4:5132087-5132106 | MS.gene050822:CDS | 45.0% | |
| ATAAGCAATGTAGGTGGAGG+TGG | + | chr7.4:5132764-5132783 | MS.gene050822:CDS | 45.0% | |
| CACCAACCACCATTGTCATT+AGG | - | chr7.4:5132426-5132445 | None:intergenic | 45.0% | |
| GCACCCCAATTCATTGACAT+TGG | - | chr7.4:5132839-5132858 | None:intergenic | 45.0% | |
| GCACCTTCCAATTGGAGATT+TGG | + | chr7.4:5132950-5132969 | MS.gene050822:CDS | 45.0% | |
| GCTCATGCAACTTTCTATGG+TGG | + | chr7.4:5132063-5132082 | MS.gene050822:CDS | 45.0% | |
| TCCCTAATGACAATGGTGGT+TGG | + | chr7.4:5132421-5132440 | MS.gene050822:CDS | 45.0% | |
| TGGCCAAATCTCCAATTGGA+AGG | - | chr7.4:5132956-5132975 | None:intergenic | 45.0% | |
| ! | AATGATGGGAAGTCATGTGG+TGG | + | chr7.4:5132294-5132313 | MS.gene050822:CDS | 45.0% |
| ! | AGACTGGCCAAATCTCCAAT+TGG | - | chr7.4:5132960-5132979 | None:intergenic | 45.0% |
| ! | TAACTTGTAGGTGGTGCATG+TGG | + | chr7.4:5132207-5132226 | MS.gene050822:intron | 45.0% |
| ! | TTAGGGAGTGCAAAGTTTGG+TGG | - | chr7.4:5132408-5132427 | None:intergenic | 45.0% |
| ACGCAAGGCAAGTTCCTCAA+TGG | + | chr7.4:5132337-5132356 | MS.gene050822:CDS | 50.0% | |
| GCACTCCCTAATGACAATGG+TGG | + | chr7.4:5132417-5132436 | MS.gene050822:CDS | 50.0% | |
| GTTCCTCAATGGTGTCTCAG+AGG | + | chr7.4:5132348-5132367 | MS.gene050822:CDS | 50.0% | |
| ! | TATGGTGGAAGTGATGCCTC+AGG | + | chr7.4:5132078-5132097 | MS.gene050822:CDS | 50.0% |
| GTAGGTGGTGCATGTGGGTA+TGG | + | chr7.4:5132213-5132232 | MS.gene050822:intron | 55.0% | |
| !! | GTGCCTCTGAGACACCATTG+AGG | - | chr7.4:5132354-5132373 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 5131976 | 5133003 | 5131976 | ID=MS.gene050822 |
| chr7.4 | mRNA | 5131976 | 5133003 | 5131976 | ID=MS.gene050822.t1;Parent=MS.gene050822 |
| chr7.4 | exon | 5131976 | 5132108 | 5131976 | ID=MS.gene050822.t1.exon1;Parent=MS.gene050822.t1 |
| chr7.4 | CDS | 5131976 | 5132108 | 5131976 | ID=cds.MS.gene050822.t1;Parent=MS.gene050822.t1 |
| chr7.4 | exon | 5132217 | 5132538 | 5132217 | ID=MS.gene050822.t1.exon2;Parent=MS.gene050822.t1 |
| chr7.4 | CDS | 5132217 | 5132538 | 5132217 | ID=cds.MS.gene050822.t1;Parent=MS.gene050822.t1 |
| chr7.4 | exon | 5132694 | 5133003 | 5132694 | ID=MS.gene050822.t1.exon3;Parent=MS.gene050822.t1 |
| chr7.4 | CDS | 5132694 | 5133003 | 5132694 | ID=cds.MS.gene050822.t1;Parent=MS.gene050822.t1 |
| Gene Sequence |
| Protein sequence |
