Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050823.t1 | XP_003626098.1 | 97.2 | 254 | 7 | 0 | 1 | 254 | 1 | 254 | 2.10E-144 | 521.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050823.t1 | Q9ZSI1 | 75.3 | 255 | 62 | 1 | 1 | 254 | 1 | 255 | 9.7e-114 | 411.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050823.t1 | G7KVH3 | 97.2 | 254 | 7 | 0 | 1 | 254 | 1 | 254 | 1.5e-144 | 521.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050720 | MS.gene050823 | 0.85454 | 1.15E-61 | -1.69E-46 |
| MS.gene050722 | MS.gene050823 | 0.871092 | 8.89E-67 | -1.69E-46 |
| MS.gene050723 | MS.gene050823 | 0.888489 | 5.64E-73 | -1.69E-46 |
| MS.gene050725 | MS.gene050823 | 0.886878 | 2.33E-72 | -1.69E-46 |
| MS.gene050726 | MS.gene050823 | 0.88723 | 1.71E-72 | -1.69E-46 |
| MS.gene050822 | MS.gene050823 | 0.899661 | 1.59E-77 | -1.69E-46 |
| MS.gene050823 | MS.gene066602 | 0.815814 | 7.62E-52 | -1.69E-46 |
| MS.gene050823 | MS.gene072622 | 0.902068 | 1.41E-78 | -1.69E-46 |
| MS.gene050823 | MS.gene072623 | 0.872038 | 4.32E-67 | -1.69E-46 |
| MS.gene050823 | MS.gene072625 | 0.918979 | 7.98E-87 | -1.69E-46 |
| MS.gene050823 | MS.gene072626 | 0.874855 | 4.87E-68 | -1.69E-46 |
| MS.gene050823 | MS.gene072628 | 0.898086 | 7.48E-77 | -1.69E-46 |
| MS.gene050823 | MS.gene072629 | 0.874104 | 8.76E-68 | -1.69E-46 |
| MS.gene050823 | MS.gene28619 | 0.862938 | 3.56E-64 | -1.69E-46 |
| MS.gene050823 | MS.gene28620 | 0.902085 | 1.39E-78 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050823.t1 | MTR_7g111010 | 97.244 | 254 | 7 | 0 | 1 | 254 | 1 | 254 | 0.0 | 516 |
| MS.gene050823.t1 | MTR_7g110990 | 92.520 | 254 | 4 | 1 | 1 | 254 | 1 | 239 | 3.63e-174 | 479 |
| MS.gene050823.t1 | MTR_1g085460 | 85.833 | 240 | 34 | 0 | 15 | 254 | 18 | 257 | 3.76e-158 | 439 |
| MS.gene050823.t1 | MTR_1g026020 | 62.550 | 251 | 93 | 1 | 4 | 254 | 6 | 255 | 7.08e-119 | 340 |
| MS.gene050823.t1 | MTR_5g075320 | 58.468 | 248 | 99 | 3 | 2 | 249 | 4 | 247 | 2.43e-106 | 308 |
| MS.gene050823.t1 | MTR_2g097030 | 57.708 | 253 | 99 | 4 | 1 | 248 | 4 | 253 | 2.73e-105 | 306 |
| MS.gene050823.t1 | MTR_3g094040 | 60.000 | 245 | 93 | 4 | 5 | 248 | 8 | 248 | 1.57e-104 | 303 |
| MS.gene050823.t1 | MTR_7g061720 | 57.480 | 254 | 99 | 4 | 1 | 248 | 1 | 251 | 2.03e-104 | 303 |
| MS.gene050823.t1 | MTR_3g094060 | 60.000 | 245 | 93 | 4 | 5 | 248 | 8 | 248 | 3.30e-104 | 303 |
| MS.gene050823.t1 | MTR_5g079950 | 58.824 | 255 | 95 | 5 | 1 | 248 | 1 | 252 | 4.23e-104 | 303 |
| MS.gene050823.t1 | MTR_5g041700 | 59.600 | 250 | 96 | 4 | 5 | 254 | 3 | 247 | 1.00e-103 | 301 |
| MS.gene050823.t1 | MTR_3g435430 | 61.506 | 239 | 87 | 4 | 16 | 254 | 14 | 247 | 3.88e-103 | 300 |
| MS.gene050823.t1 | MTR_4g081950 | 59.592 | 245 | 90 | 4 | 4 | 248 | 43 | 278 | 5.96e-103 | 300 |
| MS.gene050823.t1 | MTR_3g466790 | 59.184 | 245 | 96 | 3 | 5 | 249 | 10 | 250 | 2.81e-101 | 295 |
| MS.gene050823.t1 | MTR_7g011060 | 61.157 | 242 | 80 | 5 | 9 | 248 | 6 | 235 | 1.37e-100 | 293 |
| MS.gene050823.t1 | MTR_3g466760 | 58.776 | 245 | 97 | 3 | 5 | 249 | 16 | 256 | 1.28e-99 | 291 |
| MS.gene050823.t1 | MTR_4g063975 | 55.731 | 253 | 104 | 4 | 1 | 248 | 2 | 251 | 4.26e-98 | 287 |
| MS.gene050823.t1 | MTR_6g012520 | 63.964 | 222 | 68 | 4 | 27 | 248 | 28 | 237 | 7.98e-91 | 268 |
| MS.gene050823.t1 | MTR_5g011210 | 50.579 | 259 | 117 | 5 | 3 | 254 | 8 | 262 | 1.46e-86 | 258 |
| MS.gene050823.t1 | MTR_4g102450 | 50.980 | 255 | 117 | 4 | 5 | 254 | 10 | 261 | 3.31e-86 | 257 |
| MS.gene050823.t1 | MTR_7g013120 | 48.583 | 247 | 119 | 4 | 9 | 254 | 14 | 253 | 1.38e-84 | 253 |
| MS.gene050823.t1 | MTR_7g013140 | 51.417 | 247 | 114 | 4 | 9 | 254 | 10 | 251 | 3.85e-84 | 251 |
| MS.gene050823.t1 | MTR_7g013150 | 49.603 | 252 | 118 | 5 | 4 | 254 | 10 | 253 | 9.42e-84 | 251 |
| MS.gene050823.t1 | MTR_4g124340 | 50.224 | 223 | 106 | 3 | 27 | 248 | 61 | 279 | 7.53e-83 | 249 |
| MS.gene050823.t1 | MTR_8g464350 | 48.425 | 254 | 120 | 6 | 7 | 254 | 6 | 254 | 9.77e-81 | 243 |
| MS.gene050823.t1 | MTR_8g058990 | 49.782 | 229 | 109 | 4 | 27 | 254 | 30 | 253 | 5.84e-77 | 233 |
| MS.gene050823.t1 | MTR_0040s0140 | 46.000 | 250 | 131 | 3 | 1 | 249 | 6 | 252 | 2.87e-75 | 229 |
| MS.gene050823.t1 | MTR_2g090255 | 45.200 | 250 | 131 | 4 | 1 | 249 | 4 | 248 | 7.26e-73 | 223 |
| MS.gene050823.t1 | MTR_5g076950 | 48.000 | 225 | 110 | 3 | 27 | 249 | 41 | 260 | 1.35e-70 | 218 |
| MS.gene050823.t1 | MTR_3g064510 | 46.288 | 229 | 116 | 5 | 27 | 249 | 53 | 280 | 9.51e-67 | 208 |
| MS.gene050823.t1 | MTR_6g012500 | 57.292 | 96 | 40 | 1 | 153 | 248 | 181 | 275 | 2.30e-31 | 117 |
| MS.gene050823.t1 | MTR_0008s0120 | 31.349 | 252 | 112 | 7 | 4 | 254 | 8 | 199 | 1.16e-30 | 113 |
| MS.gene050823.t1 | MTR_2g090270 | 30.962 | 239 | 139 | 8 | 27 | 254 | 35 | 258 | 1.23e-30 | 114 |
| MS.gene050823.t1 | MTR_2g090265 | 31.799 | 239 | 137 | 9 | 27 | 254 | 33 | 256 | 3.10e-29 | 111 |
| MS.gene050823.t1 | MTR_0122s0020 | 31.381 | 239 | 138 | 9 | 27 | 254 | 41 | 264 | 1.43e-26 | 104 |
| MS.gene050823.t1 | MTR_0118s0070 | 33.610 | 241 | 124 | 11 | 32 | 254 | 53 | 275 | 3.57e-26 | 103 |
| MS.gene050823.t1 | MTR_1g085365 | 80.000 | 55 | 11 | 0 | 198 | 252 | 4 | 58 | 5.41e-25 | 94.4 |
| MS.gene050823.t1 | MTR_4g028640 | 32.677 | 254 | 134 | 12 | 17 | 254 | 32 | 264 | 1.64e-24 | 99.0 |
| MS.gene050823.t1 | MTR_4g099370 | 27.586 | 261 | 157 | 11 | 5 | 254 | 11 | 250 | 3.47e-22 | 92.4 |
| MS.gene050823.t1 | MTR_0809s0010 | 30.041 | 243 | 128 | 12 | 27 | 250 | 47 | 266 | 1.04e-21 | 91.3 |
| MS.gene050823.t1 | MTR_5g013440 | 27.132 | 258 | 162 | 11 | 5 | 254 | 12 | 251 | 3.02e-20 | 87.0 |
| MS.gene050823.t1 | MTR_4g075790 | 30.802 | 237 | 137 | 11 | 28 | 254 | 24 | 243 | 2.18e-17 | 79.3 |
| MS.gene050823.t1 | MTR_4g098930 | 27.925 | 265 | 165 | 11 | 1 | 253 | 1 | 251 | 5.20e-17 | 78.2 |
| MS.gene050823.t1 | MTR_0122s0020 | 31.818 | 176 | 94 | 9 | 27 | 191 | 41 | 201 | 5.94e-14 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050823.t1 | AT4G01630 | 75.294 | 255 | 62 | 1 | 1 | 254 | 1 | 255 | 1.18e-146 | 410 |
| MS.gene050823.t1 | AT1G20190 | 67.411 | 224 | 72 | 1 | 31 | 254 | 30 | 252 | 1.85e-111 | 321 |
| MS.gene050823.t1 | AT2G40610 | 59.917 | 242 | 94 | 2 | 8 | 249 | 11 | 249 | 7.84e-107 | 309 |
| MS.gene050823.t1 | AT5G05290 | 56.911 | 246 | 97 | 3 | 10 | 249 | 9 | 251 | 3.06e-105 | 305 |
| MS.gene050823.t1 | AT1G69530 | 58.943 | 246 | 96 | 4 | 4 | 248 | 3 | 244 | 1.89e-102 | 298 |
| MS.gene050823.t1 | AT1G69530 | 58.943 | 246 | 96 | 4 | 4 | 248 | 3 | 244 | 1.89e-102 | 298 |
| MS.gene050823.t1 | AT1G69530 | 58.943 | 246 | 96 | 4 | 4 | 248 | 3 | 244 | 2.54e-102 | 299 |
| MS.gene050823.t1 | AT3G55500 | 59.274 | 248 | 95 | 5 | 4 | 248 | 9 | 253 | 6.00e-102 | 297 |
| MS.gene050823.t1 | AT5G02260 | 56.175 | 251 | 102 | 4 | 3 | 248 | 4 | 251 | 1.71e-101 | 296 |
| MS.gene050823.t1 | AT2G03090 | 56.967 | 244 | 101 | 3 | 5 | 248 | 8 | 247 | 1.96e-101 | 296 |
| MS.gene050823.t1 | AT5G56320 | 57.377 | 244 | 100 | 3 | 5 | 248 | 7 | 246 | 6.19e-101 | 295 |
| MS.gene050823.t1 | AT5G56320 | 57.377 | 244 | 100 | 3 | 5 | 248 | 7 | 246 | 6.19e-101 | 295 |
| MS.gene050823.t1 | AT2G28950 | 57.312 | 253 | 98 | 5 | 1 | 248 | 3 | 250 | 7.15e-101 | 294 |
| MS.gene050823.t1 | AT2G39700 | 56.972 | 251 | 102 | 4 | 1 | 248 | 3 | 250 | 9.93e-101 | 294 |
| MS.gene050823.t1 | AT5G56320 | 57.377 | 244 | 100 | 3 | 5 | 248 | 7 | 246 | 3.58e-100 | 294 |
| MS.gene050823.t1 | AT2G37640 | 62.054 | 224 | 81 | 3 | 27 | 249 | 36 | 256 | 1.15e-99 | 291 |
| MS.gene050823.t1 | AT3G29030 | 53.307 | 257 | 96 | 4 | 5 | 248 | 3 | 248 | 5.93e-97 | 284 |
| MS.gene050823.t1 | AT1G26770 | 58.506 | 241 | 96 | 3 | 8 | 248 | 7 | 243 | 2.25e-94 | 278 |
| MS.gene050823.t1 | AT1G26770 | 58.506 | 241 | 96 | 3 | 8 | 248 | 17 | 253 | 3.32e-94 | 278 |
| MS.gene050823.t1 | AT3G15370 | 52.191 | 251 | 112 | 3 | 1 | 250 | 3 | 246 | 9.78e-91 | 268 |
| MS.gene050823.t1 | AT1G20190 | 64.767 | 193 | 67 | 1 | 62 | 254 | 2 | 193 | 1.90e-90 | 265 |
| MS.gene050823.t1 | AT1G69530 | 57.709 | 227 | 89 | 5 | 4 | 229 | 3 | 223 | 9.55e-90 | 266 |
| MS.gene050823.t1 | AT1G69530 | 59.361 | 219 | 82 | 5 | 4 | 221 | 3 | 215 | 4.02e-89 | 263 |
| MS.gene050823.t1 | AT2G37640 | 58.937 | 207 | 81 | 3 | 44 | 249 | 28 | 231 | 4.12e-86 | 256 |
| MS.gene050823.t1 | AT1G12560 | 50.198 | 253 | 119 | 5 | 3 | 254 | 15 | 261 | 6.24e-85 | 254 |
| MS.gene050823.t1 | AT3G15370 | 46.619 | 281 | 112 | 4 | 1 | 250 | 3 | 276 | 4.08e-84 | 253 |
| MS.gene050823.t1 | AT5G39270 | 51.316 | 228 | 105 | 4 | 27 | 254 | 58 | 279 | 5.51e-80 | 242 |
| MS.gene050823.t1 | AT1G62980 | 46.875 | 256 | 130 | 5 | 1 | 254 | 5 | 256 | 3.42e-79 | 239 |
| MS.gene050823.t1 | AT5G39290 | 50.877 | 228 | 106 | 4 | 27 | 254 | 58 | 279 | 1.57e-78 | 238 |
| MS.gene050823.t1 | AT5G39300 | 50.000 | 228 | 108 | 4 | 27 | 254 | 55 | 276 | 1.63e-78 | 238 |
| MS.gene050823.t1 | AT5G39310 | 49.362 | 235 | 112 | 5 | 21 | 254 | 84 | 312 | 2.44e-78 | 239 |
| MS.gene050823.t1 | AT5G39280 | 49.561 | 228 | 109 | 4 | 27 | 254 | 54 | 275 | 5.71e-78 | 237 |
| MS.gene050823.t1 | AT4G38210 | 44.444 | 252 | 131 | 5 | 1 | 249 | 5 | 250 | 9.51e-70 | 215 |
| MS.gene050823.t1 | AT3G03220 | 44.889 | 225 | 117 | 3 | 27 | 249 | 40 | 259 | 1.36e-67 | 210 |
| MS.gene050823.t1 | AT5G39260 | 45.852 | 229 | 112 | 5 | 27 | 254 | 45 | 262 | 2.04e-62 | 197 |
| MS.gene050823.t1 | AT4G28250 | 31.599 | 269 | 154 | 9 | 1 | 254 | 7 | 260 | 1.82e-32 | 119 |
| MS.gene050823.t1 | AT2G20750 | 32.714 | 269 | 149 | 10 | 6 | 254 | 11 | 267 | 1.47e-31 | 117 |
| MS.gene050823.t1 | AT1G65680 | 34.711 | 242 | 129 | 11 | 27 | 254 | 47 | 273 | 1.16e-29 | 112 |
| MS.gene050823.t1 | AT4G28250 | 30.597 | 268 | 152 | 9 | 1 | 254 | 7 | 254 | 2.89e-29 | 111 |
| MS.gene050823.t1 | AT2G45110 | 33.745 | 243 | 124 | 11 | 30 | 254 | 36 | 259 | 1.42e-23 | 96.3 |
| MS.gene050823.t1 | AT1G65681 | 31.718 | 227 | 126 | 11 | 41 | 254 | 12 | 222 | 4.40e-21 | 89.0 |
| MS.gene050823.t1 | AT2G20750 | 32.683 | 205 | 107 | 9 | 6 | 191 | 11 | 203 | 2.24e-19 | 84.3 |
| MS.gene050823.t1 | AT4G28250 | 29.665 | 209 | 114 | 8 | 1 | 196 | 7 | 195 | 9.56e-18 | 79.7 |
| MS.gene050823.t1 | AT4G28250 | 29.902 | 204 | 110 | 8 | 1 | 191 | 7 | 190 | 4.76e-17 | 77.4 |
| MS.gene050823.t1 | AT4G17030 | 24.710 | 259 | 176 | 8 | 1 | 252 | 1 | 247 | 2.68e-16 | 76.3 |
| MS.gene050823.t1 | AT3G60570 | 28.395 | 243 | 140 | 10 | 27 | 254 | 34 | 257 | 3.72e-15 | 73.6 |
| MS.gene050823.t1 | AT4G38400 | 26.906 | 223 | 124 | 9 | 50 | 254 | 47 | 248 | 6.14e-14 | 70.1 |
| MS.gene050823.t1 | AT3G45970 | 28.261 | 230 | 123 | 11 | 45 | 254 | 14 | 221 | 2.13e-13 | 68.2 |
| MS.gene050823.t1 | AT3G45960 | 27.315 | 216 | 132 | 10 | 50 | 254 | 46 | 247 | 9.35e-12 | 63.9 |
| MS.gene050823.t1 | AT3G45970 | 27.556 | 225 | 121 | 11 | 50 | 254 | 46 | 248 | 9.03e-11 | 60.8 |
Find 61 sgRNAs with CRISPR-Local
Find 84 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACATAAGATTTACCATAAA+TGG | 0.113150 | 7.4:+5136993 | MS.gene050823:CDS |
| AAAATAGTCTCTCCCATTTA+TGG | 0.154353 | 7.4:-5137006 | None:intergenic |
| ATTTCTAAAGTTTGGATAAA+AGG | 0.197999 | 7.4:+5137068 | MS.gene050823:CDS |
| GTGGAGAGATTTCTAAAGTT+TGG | 0.231344 | 7.4:+5137060 | MS.gene050823:CDS |
| GACATACCTCCTGTACAAAA+TGG | 0.240122 | 7.4:-5136625 | None:intergenic |
| GCACCTTCCAATTGGAGATT+TGG | 0.278944 | 7.4:+5137227 | MS.gene050823:CDS |
| CAAGCTCATGCAACTTTCTA+TGG | 0.321631 | 7.4:+5136162 | MS.gene050823:CDS |
| GGGAACCGATGTCAATGAAT+TGG | 0.333852 | 7.4:+5137108 | MS.gene050823:CDS |
| ACCAACCACCATTGTCATTA+GGG | 0.336869 | 7.4:-5136524 | None:intergenic |
| CAATGAATTGGGGTGCTAAT+TGG | 0.343861 | 7.4:+5137120 | MS.gene050823:CDS |
| CACCAACCACCATTGTCATT+AGG | 0.351626 | 7.4:-5136525 | None:intergenic |
| AATCTATACACAGATGGATA+TGG | 0.375722 | 7.4:+5136339 | MS.gene050823:CDS |
| CAAAGCCTAAGCTATCTTAA+TGG | 0.399217 | 7.4:+5137143 | MS.gene050823:CDS |
| TTTCTAAAGTTTGGATAAAA+GGG | 0.419153 | 7.4:+5137069 | MS.gene050823:CDS |
| AGTACTGCTCTCTTTAATGA+TGG | 0.424107 | 7.4:+5136381 | MS.gene050823:CDS |
| GGAACCGATGTCAATGAATT+GGG | 0.438192 | 7.4:+5137109 | MS.gene050823:CDS |
| TTAATGTTGCACCTTCCAAT+TGG | 0.443270 | 7.4:+5137219 | MS.gene050823:CDS |
| TCATTAGGGAGTGCAAAGTT+TGG | 0.443320 | 7.4:-5136510 | None:intergenic |
| TCAGAGTAGAATCTGCTGTT+TGG | 0.451820 | 7.4:+5136136 | MS.gene050823:CDS |
| GCTCCCATTAAGTTCAGAAC+TGG | 0.456142 | 7.4:-5137271 | None:intergenic |
| GACATATCAAAGTGAGGTCT+AGG | 0.457100 | 7.4:-5136555 | None:intergenic |
| ACATACCTCCTGTACAAAAT+GGG | 0.473578 | 7.4:-5136624 | None:intergenic |
| ACATAAGATTTACCATAAAT+GGG | 0.475947 | 7.4:+5136994 | MS.gene050823:CDS |
| TCTGACCATTAAGATAGCTT+AGG | 0.503897 | 7.4:-5137148 | None:intergenic |
| AGTGATGCCTCAGGAACAAT+GGG | 0.514794 | 7.4:+5136189 | MS.gene050823:CDS |
| AGACTGGCCAAATCTCCAAT+TGG | 0.514918 | 7.4:-5137234 | None:intergenic |
| TTAGGGAGTGCAAAGTTTGG+TGG | 0.522538 | 7.4:-5136507 | None:intergenic |
| GTAGGTGGTGCATGTGGGTA+TGG | 0.533831 | 7.4:+5136315 | MS.gene050823:intron |
| ATAAGCAATGTAGGTGGAGG+TGG | 0.538451 | 7.4:+5137041 | MS.gene050823:CDS |
| CAATTTACCCATTGTTCCTG+AGG | 0.543572 | 7.4:-5136196 | None:intergenic |
| GTACTTAGCTATAGTCTGAA+AGG | 0.544966 | 7.4:-5136586 | None:intergenic |
| ACATATCAAAGTGAGGTCTA+GGG | 0.546299 | 7.4:-5136554 | None:intergenic |
| TTAGCTATAGTCTGAAAGGC+AGG | 0.550027 | 7.4:-5136582 | None:intergenic |
| ATCAGATAGTTTGTGACGCA+AGG | 0.552577 | 7.4:+5136424 | MS.gene050823:CDS |
| ACTATAGCTAAGTACAGAGC+TGG | 0.557662 | 7.4:+5136594 | MS.gene050823:CDS |
| GTACTGCTCTCTTTAATGAT+GGG | 0.561983 | 7.4:+5136382 | MS.gene050823:CDS |
| TAACTTGTAGGTGGTGCATG+TGG | 0.565894 | 7.4:+5136309 | MS.gene050823:intron |
| AAGTGATGCCTCAGGAACAA+TGG | 0.569867 | 7.4:+5136188 | MS.gene050823:CDS |
| TATGGTGGAAGTGATGCCTC+AGG | 0.571406 | 7.4:+5136180 | MS.gene050823:CDS |
| ACGCAAGGCAAGTTCCTCAA+TGG | 0.572152 | 7.4:+5136439 | MS.gene050823:CDS |
| ATTTGTGGCAGTAATAGTGA+TGG | 0.572348 | 7.4:-5136478 | None:intergenic |
| TATGGAAATCTATACACAGA+TGG | 0.589533 | 7.4:+5136333 | MS.gene050823:CDS |
| CTTATAAGCAATGTAGGTGG+AGG | 0.589768 | 7.4:+5137038 | MS.gene050823:CDS |
| CATATCAAAGTGAGGTCTAG+GGG | 0.597169 | 7.4:-5136553 | None:intergenic |
| TTGGTGCTTATAAGCAATGT+AGG | 0.601676 | 7.4:+5137032 | MS.gene050823:CDS |
| GCACCCCAATTCATTGACAT+CGG | 0.603873 | 7.4:-5137113 | None:intergenic |
| TGGCCAAATCTCCAATTGGA+AGG | 0.609473 | 7.4:-5137230 | None:intergenic |
| GCTCATGCAACTTTCTATGG+TGG | 0.611814 | 7.4:+5136165 | MS.gene050823:CDS |
| TCCCTAATGACAATGGTGGT+TGG | 0.616216 | 7.4:+5136523 | MS.gene050823:CDS |
| AGAGTTGGGTGCAAAAGAAG+TGG | 0.645968 | 7.4:+5136969 | MS.gene050823:intron |
| AACTTGTAGGTGGTGCATGT+GGG | 0.650230 | 7.4:+5136310 | MS.gene050823:intron |
| GTTCCTCAATGGTGTCTCAG+AGG | 0.656491 | 7.4:+5136450 | MS.gene050823:CDS |
| GTGCTTATAAGCAATGTAGG+TGG | 0.665110 | 7.4:+5137035 | MS.gene050823:CDS |
| TTTGCACTCCCTAATGACAA+TGG | 0.665294 | 7.4:+5136516 | MS.gene050823:CDS |
| TTTAATGATGGGAAGTCATG+TGG | 0.667500 | 7.4:+5136393 | MS.gene050823:CDS |
| GCACTCCCTAATGACAATGG+TGG | 0.672667 | 7.4:+5136519 | MS.gene050823:CDS |
| GTGCCTCTGAGACACCATTG+AGG | 0.683828 | 7.4:-5136453 | None:intergenic |
| AATGATGGGAAGTCATGTGG+TGG | 0.690106 | 7.4:+5136396 | MS.gene050823:CDS |
| ATATCAAAGTGAGGTCTAGG+GGG | 0.691110 | 7.4:-5136552 | None:intergenic |
| GGTTGAGACATATCAAAGTG+AGG | 0.694334 | 7.4:-5136561 | None:intergenic |
| GAACCGATGTCAATGAATTG+GGG | 0.713611 | 7.4:+5137110 | MS.gene050823:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACATAAGATTTACCATAAA+TGG | + | chr7.4:5136993-5137012 | MS.gene050823:CDS | 20.0% |
| !! | ACATAAGATTTACCATAAAT+GGG | + | chr7.4:5136994-5137013 | MS.gene050823:CDS | 20.0% |
| !! | ATTAGAATTCAACTCAAAAA+TGG | + | chr7.4:5137179-5137198 | MS.gene050823:CDS | 20.0% |
| !! | TAACAATTTAATTGTGTGAT+CGG | - | chr7.4:5136659-5136678 | None:intergenic | 20.0% |
| !! | TTAGAATTCAACTCAAAAAT+GGG | + | chr7.4:5137180-5137199 | MS.gene050823:CDS | 20.0% |
| !!! | ATTTCTAAAGTTTGGATAAA+AGG | + | chr7.4:5137068-5137087 | MS.gene050823:CDS | 20.0% |
| !!! | CTTAATTTTGATTAACTTGT+AGG | + | chr7.4:5136297-5136316 | MS.gene050823:intron | 20.0% |
| !!! | TTTCTAAAGTTTGGATAAAA+GGG | + | chr7.4:5137069-5137088 | MS.gene050823:CDS | 20.0% |
| ! | AAGGGTCAAAAAAGAATAAA+TGG | + | chr7.4:5137087-5137106 | MS.gene050823:CDS | 25.0% |
| ! | AGGGTCAAAAAAGAATAAAT+GGG | + | chr7.4:5137088-5137107 | MS.gene050823:CDS | 25.0% |
| ! | ATTAATTAAAGTGACACTAG+TGG | + | chr7.4:5136767-5136786 | MS.gene050823:intron | 25.0% |
| ! | GTATTTATATATGCAGAGTT+GGG | + | chr7.4:5136955-5136974 | MS.gene050823:intron | 25.0% |
| ! | TGTATTTATATATGCAGAGT+TGG | + | chr7.4:5136954-5136973 | MS.gene050823:intron | 25.0% |
| !!! | AATTTTGATTAACTTGTAGG+TGG | + | chr7.4:5136300-5136319 | MS.gene050823:intron | 25.0% |
| AAAATAGTCTCTCCCATTTA+TGG | - | chr7.4:5137009-5137028 | None:intergenic | 30.0% | |
| AATCTATACACAGATGGATA+TGG | + | chr7.4:5136339-5136358 | MS.gene050823:CDS | 30.0% | |
| AGTTTGACAATGAAACATCA+AGG | - | chr7.4:5136824-5136843 | None:intergenic | 30.0% | |
| CTAAAAAATACTTGTTCGCT+AGG | - | chr7.4:5136873-5136892 | None:intergenic | 30.0% | |
| GTTTGACAATGAAACATCAA+GGG | - | chr7.4:5136823-5136842 | None:intergenic | 30.0% | |
| TATGGAAATCTATACACAGA+TGG | + | chr7.4:5136333-5136352 | MS.gene050823:CDS | 30.0% | |
| !! | TAGTGGTCATTTTACAATGT+GGG | + | chr7.4:5136784-5136803 | MS.gene050823:intron | 30.0% |
| !!! | AGCGAACAAGTATTTTTTAG+TGG | + | chr7.4:5136873-5136892 | MS.gene050823:intron | 30.0% |
| ACATACCTCCTGTACAAAAT+GGG | - | chr7.4:5136627-5136646 | None:intergenic | 35.0% | |
| ACATATCAAAGTGAGGTCTA+GGG | - | chr7.4:5136557-5136576 | None:intergenic | 35.0% | |
| AGTACTGCTCTCTTTAATGA+TGG | + | chr7.4:5136381-5136400 | MS.gene050823:CDS | 35.0% | |
| ATTTGTGGCAGTAATAGTGA+TGG | - | chr7.4:5136481-5136500 | None:intergenic | 35.0% | |
| CAAAGCCTAAGCTATCTTAA+TGG | + | chr7.4:5137143-5137162 | MS.gene050823:CDS | 35.0% | |
| GTACTGCTCTCTTTAATGAT+GGG | + | chr7.4:5136382-5136401 | MS.gene050823:CDS | 35.0% | |
| GTACTTAGCTATAGTCTGAA+AGG | - | chr7.4:5136589-5136608 | None:intergenic | 35.0% | |
| TCTGACCATTAAGATAGCTT+AGG | - | chr7.4:5137151-5137170 | None:intergenic | 35.0% | |
| TTAATGTTGCACCTTCCAAT+TGG | + | chr7.4:5137219-5137238 | MS.gene050823:CDS | 35.0% | |
| ! | GAATTGTTCCCATTTTGTAC+AGG | + | chr7.4:5136616-5136635 | MS.gene050823:CDS | 35.0% |
| ! | GTTTGGTGGACAAAAATTTG+TGG | - | chr7.4:5136496-5136515 | None:intergenic | 35.0% |
| ! | TTGGTGCTTATAAGCAATGT+AGG | + | chr7.4:5137032-5137051 | MS.gene050823:CDS | 35.0% |
| ! | TTTAATGATGGGAAGTCATG+TGG | + | chr7.4:5136393-5136412 | MS.gene050823:CDS | 35.0% |
| !! | CTAGTGGTCATTTTACAATG+TGG | + | chr7.4:5136783-5136802 | MS.gene050823:intron | 35.0% |
| !! | GTGGAGAGATTTCTAAAGTT+TGG | + | chr7.4:5137060-5137079 | MS.gene050823:CDS | 35.0% |
| ACCAACCACCATTGTCATTA+GGG | - | chr7.4:5136527-5136546 | None:intergenic | 40.0% | |
| ACTATAGCTAAGTACAGAGC+TGG | + | chr7.4:5136594-5136613 | MS.gene050823:CDS | 40.0% | |
| ATATCAAAGTGAGGTCTAGG+GGG | - | chr7.4:5136555-5136574 | None:intergenic | 40.0% | |
| CAAGCTCATGCAACTTTCTA+TGG | + | chr7.4:5136162-5136181 | MS.gene050823:CDS | 40.0% | |
| CAATTTACCCATTGTTCCTG+AGG | - | chr7.4:5136199-5136218 | None:intergenic | 40.0% | |
| CATATCAAAGTGAGGTCTAG+GGG | - | chr7.4:5136556-5136575 | None:intergenic | 40.0% | |
| CTTATAAGCAATGTAGGTGG+AGG | + | chr7.4:5137038-5137057 | MS.gene050823:CDS | 40.0% | |
| GAACCGATGTCAATGAATTG+GGG | + | chr7.4:5137110-5137129 | MS.gene050823:CDS | 40.0% | |
| GACAATGAAACATCAAGGGA+CGG | - | chr7.4:5136819-5136838 | None:intergenic | 40.0% | |
| GACATACCTCCTGTACAAAA+TGG | - | chr7.4:5136628-5136647 | None:intergenic | 40.0% | |
| GACATATCAAAGTGAGGTCT+AGG | - | chr7.4:5136558-5136577 | None:intergenic | 40.0% | |
| GATTTGATTGATATGCAGCG+TGG | - | chr7.4:5136904-5136923 | None:intergenic | 40.0% | |
| GGAACCGATGTCAATGAATT+GGG | + | chr7.4:5137109-5137128 | MS.gene050823:CDS | 40.0% | |
| GGTTGAGACATATCAAAGTG+AGG | - | chr7.4:5136564-5136583 | None:intergenic | 40.0% | |
| GTGCTTATAAGCAATGTAGG+TGG | + | chr7.4:5137035-5137054 | MS.gene050823:CDS | 40.0% | |
| GTGTCAAACTATCAGTTCAG+TGG | - | chr7.4:5136851-5136870 | None:intergenic | 40.0% | |
| TCAGAGTAGAATCTGCTGTT+TGG | + | chr7.4:5136136-5136155 | MS.gene050823:CDS | 40.0% | |
| TTAGCTATAGTCTGAAAGGC+AGG | - | chr7.4:5136585-5136604 | None:intergenic | 40.0% | |
| TTTGCACTCCCTAATGACAA+TGG | + | chr7.4:5136516-5136535 | MS.gene050823:CDS | 40.0% | |
| ! | ATCAGATAGTTTGTGACGCA+AGG | + | chr7.4:5136424-5136443 | MS.gene050823:CDS | 40.0% |
| ! | CAATGAATTGGGGTGCTAAT+TGG | + | chr7.4:5137120-5137139 | MS.gene050823:CDS | 40.0% |
| ! | TCATTAGGGAGTGCAAAGTT+TGG | - | chr7.4:5136513-5136532 | None:intergenic | 40.0% |
| ! | TTGTTCCCATTTTGTACAGG+AGG | + | chr7.4:5136619-5136638 | MS.gene050823:CDS | 40.0% |
| !! | TGGGAGAGACTATTTTGAGT+TGG | + | chr7.4:5137013-5137032 | MS.gene050823:CDS | 40.0% |
| !!! | GGACGTTACTTTTGTAAGAC+TGG | - | chr7.4:5137253-5137272 | None:intergenic | 40.0% |
| AACTTGTAGGTGGTGCATGT+GGG | + | chr7.4:5136310-5136329 | MS.gene050823:intron | 45.0% | |
| AAGTGATGCCTCAGGAACAA+TGG | + | chr7.4:5136188-5136207 | MS.gene050823:CDS | 45.0% | |
| AGAGTTGGGTGCAAAAGAAG+TGG | + | chr7.4:5136969-5136988 | MS.gene050823:intron | 45.0% | |
| AGTGATGCCTCAGGAACAAT+GGG | + | chr7.4:5136189-5136208 | MS.gene050823:CDS | 45.0% | |
| ATAAGCAATGTAGGTGGAGG+TGG | + | chr7.4:5137041-5137060 | MS.gene050823:CDS | 45.0% | |
| CACCAACCACCATTGTCATT+AGG | - | chr7.4:5136528-5136547 | None:intergenic | 45.0% | |
| GCACCCCAATTCATTGACAT+CGG | - | chr7.4:5137116-5137135 | None:intergenic | 45.0% | |
| GCACCTTCCAATTGGAGATT+TGG | + | chr7.4:5137227-5137246 | MS.gene050823:CDS | 45.0% | |
| GCTCATGCAACTTTCTATGG+TGG | + | chr7.4:5136165-5136184 | MS.gene050823:CDS | 45.0% | |
| GGGAACCGATGTCAATGAAT+TGG | + | chr7.4:5137108-5137127 | MS.gene050823:CDS | 45.0% | |
| TCCCTAATGACAATGGTGGT+TGG | + | chr7.4:5136523-5136542 | MS.gene050823:CDS | 45.0% | |
| TGGCCAAATCTCCAATTGGA+AGG | - | chr7.4:5137233-5137252 | None:intergenic | 45.0% | |
| ! | AATGATGGGAAGTCATGTGG+TGG | + | chr7.4:5136396-5136415 | MS.gene050823:CDS | 45.0% |
| ! | AGACTGGCCAAATCTCCAAT+TGG | - | chr7.4:5137237-5137256 | None:intergenic | 45.0% |
| ! | TAACTTGTAGGTGGTGCATG+TGG | + | chr7.4:5136309-5136328 | MS.gene050823:intron | 45.0% |
| ! | TTAGGGAGTGCAAAGTTTGG+TGG | - | chr7.4:5136510-5136529 | None:intergenic | 45.0% |
| ACGCAAGGCAAGTTCCTCAA+TGG | + | chr7.4:5136439-5136458 | MS.gene050823:CDS | 50.0% | |
| GCACTCCCTAATGACAATGG+TGG | + | chr7.4:5136519-5136538 | MS.gene050823:CDS | 50.0% | |
| GTTCCTCAATGGTGTCTCAG+AGG | + | chr7.4:5136450-5136469 | MS.gene050823:CDS | 50.0% | |
| ! | TATGGTGGAAGTGATGCCTC+AGG | + | chr7.4:5136180-5136199 | MS.gene050823:CDS | 50.0% |
| GTAGGTGGTGCATGTGGGTA+TGG | + | chr7.4:5136315-5136334 | MS.gene050823:intron | 55.0% | |
| !! | GTGCCTCTGAGACACCATTG+AGG | - | chr7.4:5136456-5136475 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 5136078 | 5137280 | 5136078 | ID=MS.gene050823 |
| chr7.4 | mRNA | 5136078 | 5137280 | 5136078 | ID=MS.gene050823.t1;Parent=MS.gene050823 |
| chr7.4 | exon | 5136078 | 5136210 | 5136078 | ID=MS.gene050823.t1.exon1;Parent=MS.gene050823.t1 |
| chr7.4 | CDS | 5136078 | 5136210 | 5136078 | ID=cds.MS.gene050823.t1;Parent=MS.gene050823.t1 |
| chr7.4 | exon | 5136319 | 5136640 | 5136319 | ID=MS.gene050823.t1.exon2;Parent=MS.gene050823.t1 |
| chr7.4 | CDS | 5136319 | 5136640 | 5136319 | ID=cds.MS.gene050823.t1;Parent=MS.gene050823.t1 |
| chr7.4 | exon | 5136971 | 5137280 | 5136971 | ID=MS.gene050823.t1.exon3;Parent=MS.gene050823.t1 |
| chr7.4 | CDS | 5136971 | 5137280 | 5136971 | ID=cds.MS.gene050823.t1;Parent=MS.gene050823.t1 |
| Gene Sequence |
| Protein sequence |
