Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28810.t1 | XP_003625969.1 | 96.9 | 127 | 3 | 1 | 1 | 126 | 1 | 127 | 8.00E-60 | 239.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28810.t1 | Q9LZP9 | 59.4 | 133 | 46 | 5 | 1 | 127 | 1 | 131 | 6.7e-31 | 134.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28810.t1 | G7KST6 | 96.9 | 127 | 3 | 1 | 1 | 126 | 1 | 127 | 5.8e-60 | 239.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050404 | MS.gene28810 | 0.876886 | 9.78E-69 | -1.69E-46 |
| MS.gene050406 | MS.gene28810 | 0.888187 | 7.38E-73 | -1.69E-46 |
| MS.gene050529 | MS.gene28810 | 0.80991 | 1.50E-50 | -1.69E-46 |
| MS.gene050781 | MS.gene28810 | 0.808843 | 2.54E-50 | -1.69E-46 |
| MS.gene051244 | MS.gene28810 | 0.809751 | 1.62E-50 | -1.69E-46 |
| MS.gene053971 | MS.gene28810 | 0.818388 | 2.01E-52 | -1.69E-46 |
| MS.gene054421 | MS.gene28810 | -0.804979 | 1.67E-49 | -1.69E-46 |
| MS.gene054884 | MS.gene28810 | -0.830999 | 2.14E-55 | -1.69E-46 |
| MS.gene055370 | MS.gene28810 | 0.822182 | 2.72E-53 | -1.69E-46 |
| MS.gene056633 | MS.gene28810 | 0.863556 | 2.29E-64 | -1.69E-46 |
| MS.gene056664 | MS.gene28810 | 0.844955 | 5.49E-59 | -1.69E-46 |
| MS.gene056678 | MS.gene28810 | -0.800991 | 1.11E-48 | -1.69E-46 |
| MS.gene057196 | MS.gene28810 | 0.842446 | 2.57E-58 | -1.69E-46 |
| MS.gene057246 | MS.gene28810 | 0.835062 | 2.09E-56 | -1.69E-46 |
| MS.gene057332 | MS.gene28810 | 0.810716 | 1.00E-50 | -1.69E-46 |
| MS.gene057658 | MS.gene28810 | 0.818817 | 1.61E-52 | -1.69E-46 |
| MS.gene058233 | MS.gene28810 | 0.832571 | 8.76E-56 | -1.69E-46 |
| MS.gene058276 | MS.gene28810 | 0.802535 | 5.36E-49 | -1.69E-46 |
| MS.gene058746 | MS.gene28810 | 0.848497 | 5.90E-60 | -1.69E-46 |
| MS.gene058838 | MS.gene28810 | 0.801425 | 9.06E-49 | -1.69E-46 |
| MS.gene05914 | MS.gene28810 | 0.814013 | 1.91E-51 | -1.69E-46 |
| MS.gene061150 | MS.gene28810 | 0.803953 | 2.73E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28810.t1 | MTR_7g109520 | 96.094 | 128 | 4 | 1 | 1 | 127 | 1 | 128 | 6.92e-88 | 251 |
| MS.gene28810.t1 | MTR_1g087030 | 64.615 | 130 | 41 | 3 | 2 | 127 | 3 | 131 | 6.47e-54 | 166 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28810.t1 | AT3G62410 | 58.647 | 133 | 47 | 5 | 1 | 127 | 1 | 131 | 1.48e-45 | 144 |
| MS.gene28810.t1 | AT2G47400 | 68.041 | 97 | 27 | 2 | 33 | 127 | 30 | 124 | 4.72e-40 | 130 |
| MS.gene28810.t1 | AT1G76560 | 47.297 | 74 | 37 | 1 | 56 | 127 | 61 | 134 | 6.66e-19 | 77.0 |
Find 39 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCTTCTTTGATATCTGTTC+TGG | 0.263996 | 7.4:-5877598 | None:intergenic |
| TGTTGTTGGTGAGATCTTGA+TGG | 0.363034 | 7.4:-5877506 | None:intergenic |
| AAGCTTCTCTTCAACTCATC+AGG | 0.395970 | 7.4:+5877472 | MS.gene28810:CDS |
| GCTACGCATTCTCCACTAAC+CGG | 0.412486 | 7.4:-5877664 | None:intergenic |
| GATGAGTTGAAGAGAAGCTT+GGG | 0.422285 | 7.4:-5877469 | None:intergenic |
| TGTTCTGGTGCAGCACGAAC+TGG | 0.440452 | 7.4:-5877583 | None:intergenic |
| CACCATCATTCGACCTGATC+CGG | 0.449625 | 7.4:-5877554 | None:intergenic |
| CGGTGGGGTGTAGCCGGATC+AGG | 0.450264 | 7.4:+5877541 | MS.gene28810:CDS |
| CGTGTCTGCAGTGGTGTTGT+TGG | 0.454736 | 7.4:-5877520 | None:intergenic |
| GGTGAGATCTTGATGGTTTG+TGG | 0.456742 | 7.4:-5877499 | None:intergenic |
| TGGAGAATGCGTAGCGGCAT+GGG | 0.486435 | 7.4:+5877672 | MS.gene28810:CDS |
| TGTGCTGATGACCCGGTTAG+TGG | 0.494167 | 7.4:+5877652 | MS.gene28810:CDS |
| GAAGAAGGATTCTGATCCAT+TGG | 0.495582 | 7.4:+5877741 | MS.gene28810:CDS |
| CGGCGAGTCATGCAAGGGAT+AGG | 0.497910 | 7.4:+5877719 | MS.gene28810:CDS |
| GTGGAGAATGCGTAGCGGCA+TGG | 0.516210 | 7.4:+5877671 | MS.gene28810:CDS |
| CAGACACGGTGGGGTGTAGC+CGG | 0.517241 | 7.4:+5877535 | MS.gene28810:CDS |
| TGATGAGTTGAAGAGAAGCT+TGG | 0.520626 | 7.4:-5877470 | None:intergenic |
| AGAGAAGCTTGGGGTTTGAA+AGG | 0.525586 | 7.4:-5877459 | None:intergenic |
| TCCATTGGAGGATTACTGTA+AGG | 0.532128 | 7.4:+5877756 | MS.gene28810:CDS |
| GTGCTGCGGCGAGTCATGCA+AGG | 0.540725 | 7.4:+5877713 | MS.gene28810:CDS |
| CGTAGCGGCATGGGATGAAG+TGG | 0.569852 | 7.4:+5877681 | MS.gene28810:CDS |
| TCATGCAAGGGATAGGAAGA+AGG | 0.572718 | 7.4:+5877726 | MS.gene28810:CDS |
| ACCAGAACAGATATCAAAGA+AGG | 0.574036 | 7.4:+5877597 | MS.gene28810:CDS |
| AGAGACATGTGCTGATGACC+CGG | 0.581174 | 7.4:+5877645 | MS.gene28810:CDS |
| TCCTTACAGTAATCCTCCAA+TGG | 0.581674 | 7.4:-5877757 | None:intergenic |
| AGCCGGATCAGGTCGAATGA+TGG | 0.587328 | 7.4:+5877552 | MS.gene28810:CDS |
| CTACGCATTCTCCACTAACC+GGG | 0.589171 | 7.4:-5877663 | None:intergenic |
| CAACAACACCACTGCAGACA+CGG | 0.593172 | 7.4:+5877521 | MS.gene28810:CDS |
| GAAGGATTCTGATCCATTGG+AGG | 0.594880 | 7.4:+5877744 | MS.gene28810:CDS |
| ACACCCCACCGTGTCTGCAG+TGG | 0.598200 | 7.4:-5877529 | None:intergenic |
| AACACCACTGCAGACACGGT+GGG | 0.598529 | 7.4:+5877525 | MS.gene28810:CDS |
| ACACCACTGCAGACACGGTG+GGG | 0.612423 | 7.4:+5877526 | MS.gene28810:CDS |
| CGGATCAGGTCGAATGATGG+TGG | 0.612425 | 7.4:+5877555 | MS.gene28810:CDS |
| AGCGGCATGGGATGAAGTGG+AGG | 0.620459 | 7.4:+5877684 | MS.gene28810:CDS |
| TGCTGCGGCGAGTCATGCAA+GGG | 0.625380 | 7.4:+5877714 | MS.gene28810:CDS |
| CAACACCACTGCAGACACGG+TGG | 0.673443 | 7.4:+5877524 | MS.gene28810:CDS |
| ATGAGTTGAAGAGAAGCTTG+GGG | 0.682917 | 7.4:-5877468 | None:intergenic |
| AGTGGAGGAACTCAGTGCTG+CGG | 0.688380 | 7.4:+5877699 | MS.gene28810:CDS |
| GGTTAGTGGAGAATGCGTAG+CGG | 0.759059 | 7.4:+5877666 | MS.gene28810:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACCAGAACAGATATCAAAGA+AGG | + | chr7.4:5877597-5877616 | MS.gene28810:CDS | 35.0% | |
| ACCTTCTTTGATATCTGTTC+TGG | - | chr7.4:5877601-5877620 | None:intergenic | 35.0% | |
| AAGCTTCTCTTCAACTCATC+AGG | + | chr7.4:5877472-5877491 | MS.gene28810:CDS | 40.0% | |
| GAAGAAGGATTCTGATCCAT+TGG | + | chr7.4:5877741-5877760 | MS.gene28810:CDS | 40.0% | |
| TCCATTGGAGGATTACTGTA+AGG | + | chr7.4:5877756-5877775 | MS.gene28810:CDS | 40.0% | |
| TCCTTACAGTAATCCTCCAA+TGG | - | chr7.4:5877760-5877779 | None:intergenic | 40.0% | |
| ! | ATGAGTTGAAGAGAAGCTTG+GGG | - | chr7.4:5877471-5877490 | None:intergenic | 40.0% |
| ! | GATGAGTTGAAGAGAAGCTT+GGG | - | chr7.4:5877472-5877491 | None:intergenic | 40.0% |
| ! | TGATGAGTTGAAGAGAAGCT+TGG | - | chr7.4:5877473-5877492 | None:intergenic | 40.0% |
| ! | TGTTGTTGGTGAGATCTTGA+TGG | - | chr7.4:5877509-5877528 | None:intergenic | 40.0% |
| ! | TTTTGCACTCATCAGTCTCA+GGG | - | chr7.4:5877786-5877805 | None:intergenic | 40.0% |
| AGAGAAGCTTGGGGTTTGAA+AGG | - | chr7.4:5877462-5877481 | None:intergenic | 45.0% | |
| GAAGGATTCTGATCCATTGG+AGG | + | chr7.4:5877744-5877763 | MS.gene28810:CDS | 45.0% | |
| TCATGCAAGGGATAGGAAGA+AGG | + | chr7.4:5877726-5877745 | MS.gene28810:CDS | 45.0% | |
| ! | GGTGAGATCTTGATGGTTTG+TGG | - | chr7.4:5877502-5877521 | None:intergenic | 45.0% |
| ! | GTTTTGCACTCATCAGTCTC+AGG | - | chr7.4:5877787-5877806 | None:intergenic | 45.0% |
| CAACAACACCACTGCAGACA+CGG | + | chr7.4:5877521-5877540 | MS.gene28810:CDS | 50.0% | |
| CACCATCATTCGACCTGATC+CGG | - | chr7.4:5877557-5877576 | None:intergenic | 50.0% | |
| CTACGCATTCTCCACTAACC+GGG | - | chr7.4:5877666-5877685 | None:intergenic | 50.0% | |
| GCTACGCATTCTCCACTAAC+CGG | - | chr7.4:5877667-5877686 | None:intergenic | 50.0% | |
| GGTTAGTGGAGAATGCGTAG+CGG | + | chr7.4:5877666-5877685 | MS.gene28810:CDS | 50.0% | |
| ! | AGAGACATGTGCTGATGACC+CGG | + | chr7.4:5877645-5877664 | MS.gene28810:CDS | 50.0% |
| AACACCACTGCAGACACGGT+GGG | + | chr7.4:5877525-5877544 | MS.gene28810:CDS | 55.0% | |
| AGCCGGATCAGGTCGAATGA+TGG | + | chr7.4:5877552-5877571 | MS.gene28810:CDS | 55.0% | |
| AGTGGAGGAACTCAGTGCTG+CGG | + | chr7.4:5877699-5877718 | MS.gene28810:CDS | 55.0% | |
| CGGATCAGGTCGAATGATGG+TGG | + | chr7.4:5877555-5877574 | MS.gene28810:CDS | 55.0% | |
| TGGAGAATGCGTAGCGGCAT+GGG | + | chr7.4:5877672-5877691 | MS.gene28810:CDS | 55.0% | |
| TGTTCTGGTGCAGCACGAAC+TGG | - | chr7.4:5877586-5877605 | None:intergenic | 55.0% | |
| ! | TGTGCTGATGACCCGGTTAG+TGG | + | chr7.4:5877652-5877671 | MS.gene28810:CDS | 55.0% |
| !! | CGTGTCTGCAGTGGTGTTGT+TGG | - | chr7.4:5877523-5877542 | None:intergenic | 55.0% |
| ACACCACTGCAGACACGGTG+GGG | + | chr7.4:5877526-5877545 | MS.gene28810:CDS | 60.0% | |
| AGCGGCATGGGATGAAGTGG+AGG | + | chr7.4:5877684-5877703 | MS.gene28810:CDS | 60.0% | |
| CAACACCACTGCAGACACGG+TGG | + | chr7.4:5877524-5877543 | MS.gene28810:CDS | 60.0% | |
| CGGCGAGTCATGCAAGGGAT+AGG | + | chr7.4:5877719-5877738 | MS.gene28810:CDS | 60.0% | |
| CGTAGCGGCATGGGATGAAG+TGG | + | chr7.4:5877681-5877700 | MS.gene28810:CDS | 60.0% | |
| GTGGAGAATGCGTAGCGGCA+TGG | + | chr7.4:5877671-5877690 | MS.gene28810:CDS | 60.0% | |
| TGCTGCGGCGAGTCATGCAA+GGG | + | chr7.4:5877714-5877733 | MS.gene28810:CDS | 60.0% | |
| ACACCCCACCGTGTCTGCAG+TGG | - | chr7.4:5877532-5877551 | None:intergenic | 65.0% | |
| CAGACACGGTGGGGTGTAGC+CGG | + | chr7.4:5877535-5877554 | MS.gene28810:CDS | 65.0% | |
| GTGCTGCGGCGAGTCATGCA+AGG | + | chr7.4:5877713-5877732 | MS.gene28810:CDS | 65.0% | |
| CGGTGGGGTGTAGCCGGATC+AGG | + | chr7.4:5877541-5877560 | MS.gene28810:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 5877436 | 5877819 | 5877436 | ID=MS.gene28810 |
| chr7.4 | mRNA | 5877436 | 5877819 | 5877436 | ID=MS.gene28810.t1;Parent=MS.gene28810 |
| chr7.4 | exon | 5877436 | 5877819 | 5877436 | ID=MS.gene28810.t1.exon1;Parent=MS.gene28810.t1 |
| chr7.4 | CDS | 5877436 | 5877819 | 5877436 | ID=cds.MS.gene28810.t1;Parent=MS.gene28810.t1 |
| Gene Sequence |
| Protein sequence |
