AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene30698

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30698.t1 MTR_8g011850 75.000 92 23 0 1 92 555 646 3.85e-43 149
MS.gene30698.t1 MTR_8g037220 68.478 92 29 0 1 92 538 629 2.97e-40 140
MS.gene30698.t1 MTR_8g037220 68.478 92 29 0 1 92 317 408 2.99e-40 140
MS.gene30698.t1 MTR_8g011725 65.217 92 32 0 1 92 518 609 3.71e-37 131
MS.gene30698.t1 MTR_8g011950 65.556 90 31 0 3 92 528 617 2.23e-35 127
MS.gene30698.t1 MTR_5g071610 59.341 91 37 0 1 91 537 627 9.41e-33 119
MS.gene30698.t1 MTR_5g071610 59.341 91 37 0 1 91 537 627 1.08e-32 119
MS.gene30698.t1 MTR_3g072140 60.000 90 36 0 2 91 408 497 1.90e-32 118
MS.gene30698.t1 MTR_3g022930 57.609 92 39 0 1 92 540 631 2.91e-32 118
MS.gene30698.t1 MTR_2g437560 57.143 91 39 0 1 91 499 589 9.29e-32 116
MS.gene30698.t1 MTR_8g012200 55.435 92 41 0 1 92 536 627 2.09e-31 115
MS.gene30698.t1 MTR_7g025250 57.143 91 39 0 1 91 536 626 2.46e-31 115
MS.gene30698.t1 MTR_7g025250 57.143 91 39 0 1 91 536 626 2.72e-31 115
MS.gene30698.t1 MTR_7g025250 57.143 91 39 0 1 91 536 626 2.99e-31 115
MS.gene30698.t1 MTR_0468s0010 56.522 92 40 0 1 92 506 597 4.37e-31 114
MS.gene30698.t1 MTR_8g073730 53.261 92 43 0 1 92 526 617 1.73e-30 112
MS.gene30698.t1 MTR_8g012080 54.348 92 42 0 1 92 524 615 3.26e-30 112
MS.gene30698.t1 MTR_8g012180 53.261 92 43 0 1 92 311 402 5.57e-30 111
MS.gene30698.t1 MTR_8g012180 53.261 92 43 0 1 92 520 611 6.77e-30 111
MS.gene30698.t1 MTR_8g012180 53.261 92 43 0 1 92 520 611 6.96e-30 111
MS.gene30698.t1 MTR_8g011710 52.174 92 44 0 1 92 547 638 2.42e-29 109
MS.gene30698.t1 MTR_8g011710 52.174 92 44 0 1 92 547 638 2.97e-29 109
MS.gene30698.t1 MTR_8g037235 65.217 92 8 1 1 92 520 587 7.82e-29 108
MS.gene30698.t1 MTR_4g020535 54.348 92 42 0 1 92 519 610 1.57e-28 107
MS.gene30698.t1 MTR_8g012190 51.087 92 45 0 1 92 522 613 5.25e-28 105
MS.gene30698.t1 MTR_8g012190 51.087 92 45 0 1 92 516 607 5.62e-28 105
MS.gene30698.t1 MTR_4g020550 55.435 92 41 0 1 92 1001 1092 1.91e-27 104
MS.gene30698.t1 MTR_5g029950 55.844 77 34 0 16 92 8 84 5.54e-27 100
MS.gene30698.t1 MTR_6g015410 55.435 92 41 0 1 92 525 616 1.69e-26 101
MS.gene30698.t1 MTR_7g091540 53.261 92 43 0 1 92 185 276 1.75e-26 101
MS.gene30698.t1 MTR_0294s0010 53.261 92 43 0 1 92 383 474 1.87e-26 101
MS.gene30698.t1 MTR_0286s0020 52.174 92 44 0 1 92 42 133 2.80e-26 100
MS.gene30698.t1 MTR_5g036240 57.609 92 39 0 1 92 379 470 1.16e-25 99.4
MS.gene30698.t1 MTR_4g020700 52.174 92 44 0 1 92 1043 1134 1.70e-25 98.6
MS.gene30698.t1 MTR_4g020700 52.174 92 44 0 1 92 1043 1134 1.80e-25 98.6
MS.gene30698.t1 MTR_4g020640 52.222 90 43 0 1 90 1061 1150 2.48e-25 98.2
MS.gene30698.t1 MTR_4g020640 52.222 90 43 0 1 90 1061 1150 2.66e-25 98.2
MS.gene30698.t1 MTR_6g015490 51.087 92 45 0 1 92 612 703 4.22e-25 97.8
MS.gene30698.t1 MTR_5g047480 50.000 92 46 0 1 92 527 618 4.77e-25 97.4
MS.gene30698.t1 MTR_6g015490 51.087 92 45 0 1 92 386 477 4.80e-25 97.4
MS.gene30698.t1 MTR_6g015750 50.000 92 46 0 1 92 531 622 6.25e-25 97.1
MS.gene30698.t1 MTR_4g021170 53.409 88 41 0 1 88 1022 1109 6.30e-25 97.1
MS.gene30698.t1 MTR_4g020760 51.087 92 45 0 1 92 166 257 7.47e-25 97.1
MS.gene30698.t1 MTR_5g047530 50.000 92 46 0 1 92 527 618 1.04e-24 96.7
MS.gene30698.t1 MTR_4g014570 48.913 92 47 0 1 92 594 685 1.30e-24 96.3
MS.gene30698.t1 MTR_6g015475 52.174 92 44 0 1 92 560 651 1.44e-24 96.3
MS.gene30698.t1 MTR_4g020490 50.000 92 46 0 1 92 1191 1282 1.44e-24 96.3
MS.gene30698.t1 MTR_6g015405 51.136 88 43 0 1 88 566 653 1.87e-24 95.9
MS.gene30698.t1 MTR_4g015030 50.000 92 46 0 1 92 582 673 2.84e-24 95.1
MS.gene30698.t1 MTR_6g015510 50.000 92 46 0 1 92 566 657 3.83e-24 94.7
MS.gene30698.t1 MTR_4g021057 52.174 92 42 1 1 92 1063 1152 3.86e-24 94.7
MS.gene30698.t1 MTR_4g021057 52.174 92 42 1 1 92 1063 1152 4.00e-24 94.7
MS.gene30698.t1 MTR_6g015730 51.136 88 43 0 1 88 834 921 4.21e-24 94.7
MS.gene30698.t1 MTR_4g015060 50.000 92 46 0 1 92 566 657 7.22e-24 94.0
MS.gene30698.t1 MTR_6g015675 47.826 92 48 0 1 92 35 126 9.74e-24 93.6
MS.gene30698.t1 MTR_6g015430 47.826 92 48 0 1 92 386 477 1.26e-23 93.6
MS.gene30698.t1 MTR_6g015430 47.826 92 48 0 1 92 579 670 1.32e-23 93.2
MS.gene30698.t1 MTR_6g015695 47.826 92 48 0 1 92 569 660 2.25e-23 92.8
MS.gene30698.t1 MTR_6g015505 47.826 92 48 0 1 92 332 423 2.80e-23 92.4
MS.gene30698.t1 MTR_4g021095 51.087 92 43 1 1 92 908 997 3.92e-23 92.0
MS.gene30698.t1 MTR_6g015745 48.864 88 45 0 1 88 570 657 4.23e-23 92.0
MS.gene30698.t1 MTR_4g020850 50.000 92 44 1 1 92 523 612 1.15e-22 90.9
MS.gene30698.t1 MTR_4g020850 47.826 92 46 1 1 92 1250 1339 1.68e-21 87.4
MS.gene30698.t1 MTR_8g020430 53.409 88 38 1 7 91 523 610 2.10e-22 90.1
MS.gene30698.t1 MTR_6g015455 46.237 93 49 1 1 92 908 1000 2.21e-22 89.7
MS.gene30698.t1 MTR_6g015435 47.727 88 46 0 1 88 554 641 2.41e-22 89.7
MS.gene30698.t1 MTR_4g021023 50.000 92 46 0 1 92 817 908 2.44e-22 89.7
MS.gene30698.t1 MTR_6g015590 46.739 92 49 0 1 92 458 549 2.61e-22 89.7
MS.gene30698.t1 MTR_4g021023 50.000 92 46 0 1 92 1068 1159 2.77e-22 89.7
MS.gene30698.t1 MTR_4g015050 45.652 92 50 0 1 92 449 540 4.25e-22 89.0
MS.gene30698.t1 MTR_6g015665 50.000 92 46 0 1 92 610 701 4.69e-22 89.0
MS.gene30698.t1 MTR_4g014990 45.652 92 50 0 1 92 555 646 4.96e-22 89.0
MS.gene30698.t1 MTR_4g014990 45.652 92 50 0 1 92 555 646 5.03e-22 89.0
MS.gene30698.t1 MTR_6g016000 50.000 92 45 1 1 92 602 692 7.04e-22 88.6
MS.gene30698.t1 MTR_6g016000 50.000 92 45 1 1 92 602 692 7.77e-22 88.2
MS.gene30698.t1 MTR_5g036250 43.396 106 45 2 1 92 80 184 1.26e-21 84.0
MS.gene30698.t1 MTR_4g020590 48.913 92 45 1 1 92 1031 1120 9.25e-21 85.1
MS.gene30698.t1 MTR_4g020590 48.913 92 45 1 1 92 1031 1120 9.81e-21 85.1
MS.gene30698.t1 MTR_4g020590 48.913 92 45 1 1 92 1031 1120 9.99e-21 85.1
MS.gene30698.t1 MTR_4g020590 48.913 92 45 1 1 92 1024 1113 1.07e-20 85.1
MS.gene30698.t1 MTR_4g020590 48.913 92 45 1 1 92 1031 1120 1.13e-20 85.1
MS.gene30698.t1 MTR_4g020590 48.913 92 45 1 1 92 1031 1120 1.21e-20 85.1
MS.gene30698.t1 MTR_4g021027 50.000 90 43 1 1 88 289 378 1.42e-20 84.7
MS.gene30698.t1 MTR_4g415080 44.565 92 51 0 1 92 199 290 1.76e-20 83.2
MS.gene30698.t1 MTR_7g088950 43.820 89 49 1 5 92 1037 1125 8.87e-20 82.4
MS.gene30698.t1 MTR_4g023400 44.444 90 49 1 4 92 1039 1128 9.21e-20 82.4
MS.gene30698.t1 MTR_7g088950 43.820 89 49 1 5 92 1036 1124 9.23e-20 82.4
MS.gene30698.t1 MTR_7g088950 43.820 89 49 1 5 92 1002 1090 9.68e-20 82.4
MS.gene30698.t1 MTR_7g088950 43.820 89 49 1 5 92 1036 1124 9.68e-20 82.4
MS.gene30698.t1 MTR_7g088950 43.820 89 49 1 5 92 1002 1090 9.78e-20 82.4
MS.gene30698.t1 MTR_7g088950 43.820 89 49 1 5 92 1538 1626 1.01e-19 82.4
MS.gene30698.t1 MTR_7g088950 43.820 89 49 1 5 92 1538 1626 1.01e-19 82.4
MS.gene30698.t1 MTR_7g088950 43.820 89 49 1 5 92 1002 1090 1.03e-19 82.4
MS.gene30698.t1 MTR_7g088950 43.820 89 49 1 5 92 1504 1592 1.06e-19 82.4
MS.gene30698.t1 MTR_7g088950 43.820 89 49 1 5 92 1538 1626 1.06e-19 82.4
MS.gene30698.t1 MTR_4g023400 44.444 90 49 1 4 92 1039 1128 1.07e-19 82.4
MS.gene30698.t1 MTR_7g088950 43.820 89 49 1 5 92 1538 1626 1.08e-19 82.4
MS.gene30698.t1 MTR_6g015580 45.055 91 48 1 1 91 545 633 1.80e-18 78.6
MS.gene30698.t1 MTR_6g015580 45.055 91 48 1 1 91 545 633 2.01e-18 78.6
MS.gene30698.t1 MTR_6g015775 43.956 91 49 1 1 91 534 622 2.12e-18 78.6
MS.gene30698.t1 MTR_6g015580 45.055 91 48 1 1 91 379 467 2.14e-18 78.6
MS.gene30698.t1 MTR_4g021037 49.383 81 39 1 1 81 561 639 5.73e-18 77.4
MS.gene30698.t1 MTR_7g055970 46.377 69 37 0 24 92 14 82 2.75e-16 72.4
MS.gene30698.t1 MTR_3g058790 37.113 97 53 1 1 89 532 628 4.90e-16 71.6
MS.gene30698.t1 MTR_6g087320 41.758 91 49 2 3 92 536 623 5.19e-16 71.6
MS.gene30698.t1 MTR_6g015600 42.697 89 49 1 1 89 270 356 5.80e-16 71.6
MS.gene30698.t1 MTR_3g058670 35.714 98 54 1 1 89 460 557 1.73e-15 70.1
MS.gene30698.t1 MTR_3g058870 34.694 98 54 2 1 89 539 635 1.77e-15 70.1
MS.gene30698.t1 MTR_3g058880 37.113 97 53 1 1 89 538 634 4.60e-15 68.9
MS.gene30698.t1 MTR_3g058880 37.113 97 53 1 1 89 538 634 4.92e-15 68.9
MS.gene30698.t1 MTR_8g018280 33.981 103 57 2 1 92 503 605 9.36e-15 68.2
MS.gene30698.t1 MTR_8g105820 34.653 101 57 1 1 92 452 552 9.70e-15 68.2
MS.gene30698.t1 MTR_3g058720 35.417 96 55 1 1 89 540 635 3.24e-14 66.6
MS.gene30698.t1 MTR_4g081330 39.130 92 53 2 3 92 539 629 4.12e-14 66.2
MS.gene30698.t1 MTR_3g058820 36.082 97 54 2 1 89 504 600 6.72e-14 65.9
MS.gene30698.t1 MTR_2g450830 38.776 98 51 3 1 91 579 674 9.42e-14 65.5
MS.gene30698.t1 MTR_4g080060 36.264 91 57 1 3 92 536 626 1.04e-13 65.1
MS.gene30698.t1 MTR_2g037690 35.417 96 58 1 1 92 539 634 1.10e-13 65.1
MS.gene30698.t1 MTR_4g080060 36.264 91 57 1 3 92 337 427 1.12e-13 65.1
MS.gene30698.t1 MTR_4g014580 41.250 80 46 1 5 84 403 481 1.28e-13 65.1
MS.gene30698.t1 MTR_4g081280 39.326 89 53 1 5 92 503 591 1.82e-13 64.3
MS.gene30698.t1 MTR_3g058740 34.021 97 56 1 1 89 333 429 1.92e-13 64.3
MS.gene30698.t1 MTR_3g058740 34.021 97 56 1 1 89 333 429 2.09e-13 64.3
MS.gene30698.t1 MTR_3g058740 34.021 97 56 1 1 89 540 636 2.22e-13 64.3
MS.gene30698.t1 MTR_3g058740 34.021 97 56 1 1 89 529 625 2.23e-13 64.3
MS.gene30698.t1 MTR_8g433020 35.294 102 56 3 1 92 621 722 2.85e-13 63.9
MS.gene30698.t1 MTR_8g032420 35.294 102 56 3 1 92 528 629 3.04e-13 63.9
MS.gene30698.t1 MTR_5g086690 31.068 103 60 2 1 92 518 620 9.96e-13 62.4
MS.gene30698.t1 MTR_3g058840 34.000 100 58 2 1 92 34 133 1.16e-12 62.0
MS.gene30698.t1 MTR_3g058840 34.000 100 58 2 1 92 115 214 1.27e-12 62.0
MS.gene30698.t1 MTR_3g058840 35.052 97 55 2 1 89 364 460 1.40e-12 62.0
MS.gene30698.t1 MTR_8g090280 33.673 98 55 1 1 88 433 530 1.50e-12 62.0
MS.gene30698.t1 MTR_6g015680 41.304 92 54 0 1 92 562 653 1.61e-12 62.0
MS.gene30698.t1 MTR_2g037140 33.333 96 60 1 1 92 636 731 4.40e-12 60.5
MS.gene30698.t1 MTR_4g080320 36.264 91 57 1 3 92 353 443 4.93e-12 60.5
MS.gene30698.t1 MTR_4g080320 36.264 91 57 1 3 92 530 620 5.59e-12 60.5
MS.gene30698.t1 MTR_3g079780 36.190 105 51 3 1 89 539 643 1.48e-11 58.9
MS.gene30698.t1 MTR_8g018170 30.612 98 59 1 1 89 519 616 1.61e-11 58.9
MS.gene30698.t1 MTR_4g081250 34.444 90 59 0 3 92 528 617 1.66e-11 58.9
MS.gene30698.t1 MTR_4g036080 33.654 104 57 3 1 92 542 645 2.54e-11 58.5
MS.gene30698.t1 MTR_8g018040 31.633 98 57 2 1 88 508 605 2.88e-11 58.2
MS.gene30698.t1 MTR_8g042650 33.333 93 53 2 1 84 523 615 4.13e-11 57.8
MS.gene30698.t1 MTR_8g076910 32.979 94 57 1 2 89 530 623 4.38e-11 57.8
MS.gene30698.t1 MTR_8g018260 30.928 97 58 1 1 88 519 615 4.78e-11 57.8
MS.gene30698.t1 MTR_8g018480 31.579 95 59 1 1 89 543 637 8.28e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30698.t1 AT5G36930 42.045 88 51 0 1 88 523 610 1.24e-18 79.3
MS.gene30698.t1 AT5G36930 42.045 88 51 0 1 88 526 613 1.26e-18 79.3
MS.gene30698.t1 AT5G36930 42.045 88 51 0 1 88 523 610 1.31e-18 79.3
MS.gene30698.t1 AT5G36930 42.045 88 51 0 1 88 523 610 1.31e-18 79.3
MS.gene30698.t1 AT1G27170 34.247 73 48 0 15 87 588 660 1.29e-12 62.0
MS.gene30698.t1 AT1G27170 34.247 73 48 0 15 87 588 660 1.30e-12 62.0
MS.gene30698.t1 AT1G27180 33.803 71 47 0 17 87 425 495 3.65e-12 60.8
MS.gene30698.t1 AT1G27180 33.803 71 47 0 17 87 425 495 3.65e-12 60.8
MS.gene30698.t1 AT1G27180 33.803 71 47 0 17 87 425 495 3.65e-12 60.8
MS.gene30698.t1 AT1G27180 33.803 71 47 0 17 87 601 671 4.01e-12 60.8
MS.gene30698.t1 AT1G27180 33.803 71 47 0 17 87 599 669 4.08e-12 60.8
MS.gene30698.t1 AT1G27180 33.803 71 47 0 17 87 599 669 4.08e-12 60.8
MS.gene30698.t1 AT1G27180 33.803 71 47 0 17 87 601 671 4.20e-12 60.8
MS.gene30698.t1 AT1G27180 33.803 71 47 0 17 87 763 833 4.64e-12 60.5
MS.gene30698.t1 AT5G17680 32.990 97 57 2 1 89 523 619 4.57e-11 57.8
MS.gene30698.t1 AT5G17680 32.990 97 57 2 1 89 524 620 4.57e-11 57.8
MS.gene30698.t1 AT4G14370 31.313 99 59 2 2 92 482 579 6.18e-11 57.4
MS.gene30698.t1 AT4G14370 31.313 99 59 2 2 92 525 622 7.10e-11 57.4
MS.gene30698.t1 AT4G14370 31.313 99 59 2 2 92 525 622 7.10e-11 57.4

Find 7 sgRNAs with CRISPR-Local

Find 15 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGATAATGTTGATCTTAC+AGG 0.233124 6.3:+43138366 MS.gene30698:CDS
ACAGCAATATTAAACAACTA+TGG 0.374802 6.3:+43138502 MS.gene30698:CDS
ATTGAGATGGCTCCGTTGGC+GGG 0.451662 6.3:+43138413 MS.gene30698:CDS
AATTGAGATGGCTCCGTTGG+CGG 0.504161 6.3:+43138412 MS.gene30698:CDS
TTGAGATGGCTCCGTTGGCG+GGG 0.587882 6.3:+43138414 MS.gene30698:CDS
CGTATGTAAATCCCCGCCAA+CGG 0.630953 6.3:-43138425 None:intergenic
ACAACTATGGAATAAAACCA+AGG 0.691561 6.3:+43138515 MS.gene30698:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ACAGCAATATTAAACAACTA+TGG + chr6.3:43138502-43138521 MS.gene30698:CDS 25.0%
!! TATACCTGATGAATTTTATC+AGG + chr6.3:43138452-43138471 MS.gene30698:CDS 25.0%
!! TTTTCCTGATAAAATTCATC+AGG - chr6.3:43138459-43138478 None:intergenic 25.0%
ACAACTATGGAATAAAACCA+AGG + chr6.3:43138515-43138534 MS.gene30698:CDS 30.0%
ACCTTTCTCAAAAATTGAGA+TGG + chr6.3:43138400-43138419 MS.gene30698:CDS 30.0%
CAACTATGGAATAAAACCAA+GGG + chr6.3:43138516-43138535 MS.gene30698:CDS 30.0%
!! CTTGATAATGTTGATCTTAC+AGG + chr6.3:43138366-43138385 MS.gene30698:CDS 30.0%
!! GCCATCTCAATTTTTGAGAA+AGG - chr6.3:43138404-43138423 None:intergenic 35.0%
AAAAATTGAGATGGCTCCGT+TGG + chr6.3:43138409-43138428 MS.gene30698:CDS 40.0%
AACAAAAACGGTGGAAGGAT+TGG + chr6.3:43138263-43138282 MS.gene30698:CDS 40.0%
!!! GTTTTGAAGTCGCAAAGCAC+TGG + chr6.3:43138285-43138304 MS.gene30698:CDS 45.0%
AATTGAGATGGCTCCGTTGG+CGG + chr6.3:43138412-43138431 MS.gene30698:CDS 50.0%
CGTATGTAAATCCCCGCCAA+CGG - chr6.3:43138428-43138447 None:intergenic 50.0%
ATTGAGATGGCTCCGTTGGC+GGG + chr6.3:43138413-43138432 MS.gene30698:CDS 55.0%
TTGAGATGGCTCCGTTGGCG+GGG + chr6.3:43138414-43138433 MS.gene30698:CDS 60.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 43138261 43138536 43138261 ID=MS.gene30698
chr6.3 mRNA 43138261 43138536 43138261 ID=MS.gene30698.t1;Parent=MS.gene30698
chr6.3 exon 43138261 43138536 43138261 ID=MS.gene30698.t1.exon1;Parent=MS.gene30698.t1
chr6.3 CDS 43138261 43138536 43138261 ID=cds.MS.gene30698.t1;Parent=MS.gene30698.t1
Gene Sequence

>MS.gene30698

GGAACAAAAACGGTGGAAGGATTGGTTTTGAAGTCGCAAAGCACTGGAAAAGTTAGCTTCAATGCTGATTCTTTCAAGAAGATGAACAATTTGAGACTTTTACAACTTGATAATGTTGATCTTACAGGAGATTATGTGCACCTTTCTCAAAAATTGAGATGGCTCCGTTGGCGGGGATTTACATACGACTATATACCTGATGAATTTTATCAGGAAAATCTAGTCGTTTTTGAGTTAAAACACAGCAATATTAAACAACTATGGAATAAAACCAAG
Protein sequence

>MS.gene30698.t1

GTKTVEGLVLKSQSTGKVSFNADSFKKMNNLRLLQLDNVDLTGDYVHLSQKLRWLRWRGFTYDYIPDEFYQENLVVFELKHSNIKQLWNKTK