Alfalfa Gene Editing Database
Nr:
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MS.gene30973.t1 | XP_013465412.1 | 98.8 | 260 | 3 | 0 | 1 | 260 | 1 | 260 | 2.40E-151 | 544.7 |
Swissprot:
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MS.gene30973.t1 | Q38865 | 84.8 | 257 | 39 | 0 | 4 | 260 | 1 | 257 | 3.1e-131 | 469.2 |
Trembl:
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MS.gene30973.t1 | A0A072VCF4 | 98.8 | 260 | 3 | 0 | 1 | 260 | 1 | 260 | 1.7e-151 | 544.7 |
TFs/TRs:
Gene ID | Type | Classification |
---|
Protein Kinases:
Gene ID | Type | Classification |
---|
Network:
Co-expression Network:
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MS.gene052105 | MS.gene30973 | 0.995199 | 6.04E-214 | -1.69E-46 |
PPI:
Gene1 | Gene2 | Type |
---|---|---|
MS.gene30973 | MS.gene025741 | PPI |
MS.gene20357 | MS.gene30973 | PPI |
MS.gene30973 | MS.gene37224 | PPI |
MS.gene22589 | MS.gene30973 | PPI |
MS.gene015508 | MS.gene30973 | PPI |
MS.gene62821 | MS.gene30973 | PPI |
MS.gene011449 | MS.gene30973 | PPI |
MS.gene033403 | MS.gene30973 | PPI |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MS.gene30973.t1 | MTR_2g097030 | 98.846 | 260 | 3 | 0 | 1 | 260 | 1 | 260 | 0.0 | 534 |
MS.gene30973.t1 | MTR_5g079950 | 86.694 | 248 | 33 | 0 | 13 | 260 | 12 | 259 | 6.20e-166 | 459 |
MS.gene30973.t1 | MTR_4g063975 | 87.209 | 258 | 33 | 0 | 3 | 260 | 1 | 258 | 3.86e-163 | 452 |
MS.gene30973.t1 | MTR_7g061720 | 81.783 | 258 | 47 | 0 | 3 | 260 | 1 | 258 | 3.53e-160 | 445 |
MS.gene30973.t1 | MTR_3g094060 | 68.217 | 258 | 77 | 3 | 1 | 258 | 1 | 253 | 3.99e-127 | 361 |
MS.gene30973.t1 | MTR_3g094040 | 67.451 | 255 | 79 | 2 | 6 | 258 | 1 | 253 | 1.91e-126 | 359 |
MS.gene30973.t1 | MTR_5g041700 | 72.926 | 229 | 60 | 1 | 31 | 259 | 22 | 248 | 3.59e-125 | 356 |
MS.gene30973.t1 | MTR_4g081950 | 68.093 | 257 | 72 | 4 | 1 | 255 | 32 | 280 | 2.91e-123 | 352 |
MS.gene30973.t1 | MTR_5g075320 | 67.600 | 250 | 76 | 3 | 11 | 258 | 5 | 251 | 5.18e-122 | 348 |
MS.gene30973.t1 | MTR_3g466760 | 66.275 | 255 | 80 | 2 | 4 | 258 | 12 | 260 | 3.53e-120 | 343 |
MS.gene30973.t1 | MTR_3g466790 | 66.537 | 257 | 82 | 3 | 3 | 258 | 1 | 254 | 1.18e-119 | 342 |
MS.gene30973.t1 | MTR_3g435430 | 71.111 | 225 | 63 | 1 | 34 | 258 | 25 | 247 | 5.67e-119 | 340 |
MS.gene30973.t1 | MTR_7g011060 | 63.563 | 247 | 80 | 2 | 12 | 258 | 4 | 240 | 4.42e-112 | 322 |
MS.gene30973.t1 | MTR_1g085460 | 66.079 | 227 | 73 | 3 | 33 | 256 | 29 | 254 | 1.61e-107 | 311 |
MS.gene30973.t1 | MTR_1g026020 | 58.984 | 256 | 99 | 3 | 3 | 256 | 1 | 252 | 3.26e-107 | 310 |
MS.gene30973.t1 | MTR_7g111010 | 59.289 | 253 | 95 | 4 | 4 | 253 | 1 | 248 | 6.63e-107 | 310 |
MS.gene30973.t1 | MTR_7g110990 | 63.755 | 229 | 79 | 3 | 28 | 253 | 6 | 233 | 2.98e-105 | 305 |
MS.gene30973.t1 | MTR_4g124340 | 59.031 | 227 | 90 | 2 | 32 | 257 | 59 | 283 | 7.25e-102 | 298 |
MS.gene30973.t1 | MTR_6g012520 | 61.265 | 253 | 87 | 3 | 6 | 258 | 1 | 242 | 1.92e-99 | 290 |
MS.gene30973.t1 | MTR_7g013150 | 55.556 | 252 | 105 | 2 | 5 | 256 | 6 | 250 | 3.97e-97 | 285 |
MS.gene30973.t1 | MTR_8g464350 | 54.065 | 246 | 111 | 1 | 8 | 253 | 5 | 248 | 5.96e-96 | 282 |
MS.gene30973.t1 | MTR_7g013140 | 53.725 | 255 | 110 | 2 | 1 | 255 | 1 | 247 | 6.23e-95 | 279 |
MS.gene30973.t1 | MTR_8g058990 | 57.658 | 222 | 92 | 1 | 34 | 255 | 30 | 249 | 8.20e-94 | 276 |
MS.gene30973.t1 | MTR_7g013120 | 56.696 | 224 | 94 | 2 | 34 | 256 | 29 | 250 | 3.11e-93 | 275 |
MS.gene30973.t1 | MTR_4g102450 | 51.383 | 253 | 120 | 3 | 9 | 259 | 11 | 262 | 4.99e-92 | 272 |
MS.gene30973.t1 | MTR_5g011210 | 50.602 | 249 | 121 | 2 | 9 | 256 | 12 | 259 | 6.93e-92 | 272 |
MS.gene30973.t1 | MTR_5g076950 | 55.111 | 225 | 100 | 1 | 34 | 258 | 41 | 264 | 3.88e-88 | 263 |
MS.gene30973.t1 | MTR_0040s0140 | 46.825 | 252 | 131 | 1 | 7 | 258 | 8 | 256 | 1.37e-86 | 258 |
MS.gene30973.t1 | MTR_2g090255 | 44.961 | 258 | 136 | 1 | 1 | 258 | 1 | 252 | 7.59e-85 | 254 |
MS.gene30973.t1 | MTR_3g064510 | 53.617 | 235 | 100 | 5 | 33 | 259 | 52 | 285 | 2.61e-81 | 246 |
MS.gene30973.t1 | MTR_0008s0120 | 60.909 | 110 | 43 | 0 | 146 | 255 | 86 | 195 | 1.45e-41 | 141 |
MS.gene30973.t1 | MTR_0008s0120 | 52.941 | 85 | 37 | 1 | 6 | 90 | 7 | 88 | 5.54e-22 | 90.9 |
MS.gene30973.t1 | MTR_6g012500 | 42.286 | 175 | 85 | 3 | 100 | 258 | 106 | 280 | 3.23e-37 | 132 |
MS.gene30973.t1 | MTR_2g090270 | 28.030 | 264 | 163 | 10 | 5 | 256 | 7 | 255 | 1.68e-23 | 96.3 |
MS.gene30973.t1 | MTR_0122s0020 | 30.638 | 235 | 137 | 11 | 34 | 256 | 41 | 261 | 6.47e-21 | 89.4 |
MS.gene30973.t1 | MTR_2g090265 | 27.863 | 262 | 160 | 11 | 10 | 256 | 6 | 253 | 1.46e-20 | 88.2 |
MS.gene30973.t1 | MTR_4g028640 | 29.661 | 236 | 131 | 11 | 34 | 253 | 42 | 258 | 2.19e-19 | 85.1 |
MS.gene30973.t1 | MTR_0118s0070 | 29.870 | 231 | 132 | 12 | 39 | 253 | 53 | 269 | 3.74e-17 | 79.3 |
MS.gene30973.t1 | MTR_1g085365 | 57.143 | 56 | 24 | 0 | 203 | 258 | 4 | 59 | 9.29e-14 | 64.7 |
MS.gene30973.t1 | MTR_0122s0020 | 32.022 | 178 | 96 | 10 | 34 | 200 | 41 | 204 | 7.91e-13 | 66.2 |
MS.gene30973.t1 | MTR_4g075790 | 26.667 | 225 | 130 | 9 | 45 | 253 | 32 | 237 | 2.40e-12 | 65.5 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MS.gene30973.t1 | AT2G39700 | 83.137 | 255 | 42 | 1 | 7 | 260 | 3 | 257 | 2.57e-163 | 452 |
MS.gene30973.t1 | AT2G28950 | 84.825 | 257 | 39 | 0 | 4 | 260 | 1 | 257 | 1.15e-159 | 443 |
MS.gene30973.t1 | AT3G55500 | 76.923 | 260 | 60 | 0 | 1 | 260 | 1 | 260 | 3.80e-155 | 432 |
MS.gene30973.t1 | AT2G37640 | 78.486 | 251 | 54 | 0 | 10 | 260 | 12 | 262 | 1.89e-151 | 423 |
MS.gene30973.t1 | AT5G02260 | 75.194 | 258 | 64 | 0 | 3 | 260 | 1 | 258 | 4.06e-149 | 417 |
MS.gene30973.t1 | AT2G37640 | 80.952 | 210 | 40 | 0 | 51 | 260 | 28 | 237 | 2.95e-130 | 368 |
MS.gene30973.t1 | AT1G69530 | 67.729 | 251 | 78 | 2 | 9 | 259 | 3 | 250 | 1.56e-122 | 349 |
MS.gene30973.t1 | AT1G69530 | 67.729 | 251 | 78 | 2 | 9 | 259 | 3 | 250 | 1.56e-122 | 349 |
MS.gene30973.t1 | AT1G69530 | 67.331 | 251 | 79 | 2 | 9 | 259 | 3 | 250 | 3.21e-121 | 347 |
MS.gene30973.t1 | AT5G56320 | 65.234 | 256 | 85 | 3 | 6 | 260 | 1 | 253 | 4.81e-120 | 343 |
MS.gene30973.t1 | AT5G56320 | 65.234 | 256 | 85 | 3 | 6 | 260 | 1 | 253 | 4.81e-120 | 343 |
MS.gene30973.t1 | AT2G03090 | 70.996 | 231 | 64 | 2 | 29 | 259 | 26 | 253 | 1.63e-119 | 342 |
MS.gene30973.t1 | AT5G56320 | 65.234 | 256 | 85 | 3 | 6 | 260 | 1 | 253 | 2.81e-119 | 343 |
MS.gene30973.t1 | AT5G05290 | 63.780 | 254 | 84 | 3 | 6 | 258 | 9 | 255 | 1.16e-118 | 340 |
MS.gene30973.t1 | AT2G40610 | 69.565 | 230 | 67 | 2 | 30 | 258 | 26 | 253 | 9.70e-116 | 332 |
MS.gene30973.t1 | AT1G26770 | 69.796 | 245 | 71 | 2 | 16 | 259 | 7 | 249 | 2.08e-114 | 328 |
MS.gene30973.t1 | AT1G26770 | 69.796 | 245 | 71 | 2 | 16 | 259 | 17 | 259 | 2.79e-114 | 328 |
MS.gene30973.t1 | AT3G29030 | 66.372 | 226 | 67 | 2 | 34 | 259 | 38 | 254 | 2.84e-107 | 311 |
MS.gene30973.t1 | AT4G01630 | 62.097 | 248 | 85 | 5 | 9 | 253 | 8 | 249 | 4.75e-107 | 310 |
MS.gene30973.t1 | AT1G69530 | 61.355 | 251 | 64 | 3 | 9 | 259 | 3 | 220 | 2.62e-106 | 307 |
MS.gene30973.t1 | AT1G69530 | 60.956 | 251 | 65 | 3 | 9 | 259 | 3 | 220 | 3.63e-105 | 305 |
MS.gene30973.t1 | AT1G20190 | 61.803 | 233 | 80 | 3 | 24 | 254 | 22 | 247 | 3.04e-99 | 290 |
MS.gene30973.t1 | AT5G39290 | 50.542 | 277 | 110 | 4 | 3 | 256 | 4 | 276 | 1.12e-95 | 282 |
MS.gene30973.t1 | AT5G39270 | 50.542 | 277 | 110 | 4 | 3 | 256 | 4 | 276 | 2.12e-95 | 281 |
MS.gene30973.t1 | AT1G62980 | 52.941 | 255 | 117 | 3 | 3 | 256 | 1 | 253 | 3.30e-92 | 273 |
MS.gene30973.t1 | AT5G39300 | 51.136 | 264 | 112 | 5 | 6 | 256 | 14 | 273 | 1.11e-90 | 270 |
MS.gene30973.t1 | AT5G39280 | 56.250 | 224 | 94 | 2 | 33 | 256 | 53 | 272 | 1.58e-90 | 269 |
MS.gene30973.t1 | AT1G12560 | 54.008 | 237 | 105 | 4 | 25 | 259 | 28 | 262 | 7.25e-89 | 264 |
MS.gene30973.t1 | AT3G03220 | 53.744 | 227 | 104 | 1 | 34 | 260 | 40 | 265 | 6.14e-87 | 259 |
MS.gene30973.t1 | AT5G39310 | 55.357 | 224 | 95 | 3 | 34 | 256 | 90 | 309 | 2.88e-85 | 257 |
MS.gene30973.t1 | AT4G38210 | 45.968 | 248 | 132 | 1 | 14 | 259 | 8 | 255 | 1.44e-79 | 240 |
MS.gene30973.t1 | AT1G20190 | 60.106 | 188 | 72 | 2 | 69 | 254 | 2 | 188 | 2.06e-78 | 235 |
MS.gene30973.t1 | AT3G15370 | 47.638 | 254 | 117 | 7 | 7 | 256 | 6 | 247 | 2.08e-75 | 230 |
MS.gene30973.t1 | AT5G39260 | 52.000 | 225 | 98 | 3 | 33 | 256 | 44 | 259 | 7.43e-75 | 229 |
MS.gene30973.t1 | AT3G15370 | 42.756 | 283 | 116 | 8 | 8 | 256 | 7 | 277 | 9.30e-69 | 214 |
MS.gene30973.t1 | AT4G28250 | 30.085 | 236 | 138 | 10 | 34 | 256 | 36 | 257 | 1.32e-22 | 94.0 |
MS.gene30973.t1 | AT2G20750 | 29.958 | 237 | 140 | 10 | 34 | 256 | 40 | 264 | 1.17e-21 | 91.7 |
MS.gene30973.t1 | AT1G65680 | 30.213 | 235 | 135 | 11 | 34 | 253 | 47 | 267 | 2.56e-20 | 87.8 |
MS.gene30973.t1 | AT4G28250 | 28.936 | 235 | 136 | 10 | 34 | 256 | 36 | 251 | 7.40e-19 | 83.6 |
MS.gene30973.t1 | AT2G20750 | 30.857 | 175 | 99 | 7 | 34 | 197 | 40 | 203 | 8.95e-15 | 71.6 |
MS.gene30973.t1 | AT4G28250 | 31.073 | 177 | 93 | 8 | 34 | 200 | 36 | 193 | 1.36e-13 | 68.2 |
MS.gene30973.t1 | AT4G28250 | 31.034 | 174 | 91 | 8 | 34 | 197 | 36 | 190 | 4.79e-13 | 66.6 |
MS.gene30973.t1 | AT3G60570 | 23.723 | 274 | 180 | 8 | 1 | 260 | 1 | 259 | 5.03e-13 | 67.4 |
MS.gene30973.t1 | AT3G45970 | 25.352 | 213 | 137 | 9 | 52 | 253 | 14 | 215 | 5.20e-13 | 67.0 |
MS.gene30973.t1 | AT4G17030 | 25.100 | 251 | 165 | 9 | 13 | 253 | 6 | 243 | 3.12e-12 | 65.1 |
Find 75 sgRNAs with CRISPR-Local
Find 112 sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
ACCGTCGCACTTCCACTTCT+TGG | 0.241176 | 2.2:+5117853 | MS.gene30973:CDS |
GGAACATTGCTCCACATAAT+TGG | 0.246045 | 2.2:+5117874 | MS.gene30973:CDS |
GCTCCACATAATTGGCAATT+TGG | 0.259736 | 2.2:+5117882 | MS.gene30973:CDS |
TCACTAGGTTGAGCAAAATT+AGG | 0.282502 | 2.2:-5116840 | None:intergenic |
TTGGCAATCAAATTCAGTTT+TGG | 0.302765 | 2.2:+5117794 | MS.gene30973:CDS |
GACAAGCAGTGGTGTCATTC+TGG | 0.336967 | 2.2:+5116777 | MS.gene30973:CDS |
TATGTTAAGGGCACTAAAAC+AGG | 0.337612 | 2.2:+5117738 | MS.gene30973:CDS |
GGATGCCAATGAGCAGAAAC+TGG | 0.338905 | 2.2:+5117763 | MS.gene30973:CDS |
GTTAGTTGCTGTGATGAAAA+TGG | 0.339967 | 2.2:-5116808 | None:intergenic |
TTAGGAGGAGCATGTGGATA+TGG | 0.344997 | 2.2:+5116648 | MS.gene30973:intron |
ATAAATGGGTATGGTGAGTA+TGG | 0.345079 | 2.2:+5116348 | None:intergenic |
GTCCTAATCAGCAACGTTGC+AGG | 0.348958 | 2.2:+5117693 | MS.gene30973:CDS |
GTAGGAAGCGTGGTGGGATT+AGG | 0.372081 | 2.2:+5117637 | MS.gene30973:CDS |
CACCTGCAACGTTGCTGATT+AGG | 0.375478 | 2.2:-5117695 | None:intergenic |
GCTTTGTCCTTCAGAGTTAC+CGG | 0.383935 | 2.2:+5117825 | MS.gene30973:CDS |
CAATTTGGTCAAACTTTCAC+TGG | 0.389947 | 2.2:+5117897 | MS.gene30973:CDS |
ATCAGCAACGTTGCAGGTGC+AGG | 0.391465 | 2.2:+5117699 | MS.gene30973:CDS |
GGGATTAGGTTCACAATAAA+CGG | 0.397891 | 2.2:+5117651 | MS.gene30973:CDS |
AAAGGCGACAGGGACGATTC+CGG | 0.402838 | 2.2:-5116943 | None:intergenic |
TACGGAGGTTCCGATGCTTC+CGG | 0.415576 | 2.2:+5116475 | MS.gene30973:CDS |
TATAGTGAGGACCTATGTTA+AGG | 0.434796 | 2.2:+5117725 | MS.gene30973:CDS |
AACTTGTATAGTCAAGGTTA+TGG | 0.443205 | 2.2:+5116672 | MS.gene30973:CDS |
TCGTCCCTGTCGCCTTTCGC+CGG | 0.466817 | 2.2:+5116949 | MS.gene30973:CDS |
TGACCAAATTGCCAATTATG+TGG | 0.467767 | 2.2:-5117885 | None:intergenic |
ACAAACCGGCGAAAGGCGAC+AGG | 0.470017 | 2.2:-5116954 | None:intergenic |
TCCGATGCTTCCGGCACCAT+GGG | 0.492789 | 2.2:+5116484 | MS.gene30973:CDS |
ATCCCACCACGCTTCCTACA+TGG | 0.515202 | 2.2:-5117633 | None:intergenic |
ATAGTGAGGACCTATGTTAA+GGG | 0.521329 | 2.2:+5117726 | MS.gene30973:CDS |
GCACCACCATTGTAAACACC+TGG | 0.523553 | 2.2:-5116427 | None:intergenic |
ACCCATGGTGCCGGAAGCAT+CGG | 0.533485 | 2.2:-5116485 | None:intergenic |
TGGAGCAATGTTCCAAGAAG+TGG | 0.536985 | 2.2:-5117865 | None:intergenic |
GATGCCAATGAGCAGAAACT+GGG | 0.559055 | 2.2:+5117764 | MS.gene30973:CDS |
TCAGCAACGTTGCAGGTGCA+GGG | 0.562393 | 2.2:+5117700 | MS.gene30973:CDS |
TTCCGATGCTTCCGGCACCA+TGG | 0.566242 | 2.2:+5116483 | MS.gene30973:CDS |
TTCAACAATGGACTAAGTTG+TGG | 0.574416 | 2.2:+5116726 | MS.gene30973:CDS |
GTAGGGTACAAACCGGCGAA+AGG | 0.576269 | 2.2:-5116961 | None:intergenic |
ATGGTAAATGCTAGAATCCC+AGG | 0.576542 | 2.2:+5116409 | MS.gene30973:CDS |
GGAAGTGCGACGGTCACTAC+CGG | 0.579926 | 2.2:-5117844 | None:intergenic |
CACCAACCACCATTGTCACT+AGG | 0.581152 | 2.2:-5116855 | None:intergenic |
TATGGAAACTTGTATAGTCA+AGG | 0.585100 | 2.2:+5116666 | MS.gene30973:CDS |
GTCACTACCGGTAACTCTGA+AGG | 0.588519 | 2.2:-5117832 | None:intergenic |
ATCCCAGGTGTTTACAATGG+TGG | 0.596482 | 2.2:+5116424 | MS.gene30973:CDS |
ACTAGACGTACCCATGGTGC+CGG | 0.607745 | 2.2:-5116494 | None:intergenic |
TTTGCTCAACCTAGTGACAA+TGG | 0.609073 | 2.2:+5116846 | MS.gene30973:CDS |
GCAAGATCAAAGTGAGGACG+AGG | 0.609633 | 2.2:-5116885 | None:intergenic |
TCCAAGAAGTGGAAGTGCGA+CGG | 0.611335 | 2.2:-5117854 | None:intergenic |
TATGTCACTAACAATGTGGA+TGG | 0.613645 | 2.2:+5116390 | MS.gene30973:CDS |
AACCTAGTGACAATGGTGGT+TGG | 0.618881 | 2.2:+5116853 | MS.gene30973:CDS |
CAGCAACGTTGCAGGTGCAG+GGG | 0.618959 | 2.2:+5117701 | MS.gene30973:CDS |
GATTATGCAGGGTGCCATGT+AGG | 0.619428 | 2.2:+5117619 | MS.gene30973:intron |
TGCCATGTAGGAAGCGTGGT+GGG | 0.620597 | 2.2:+5117631 | MS.gene30973:CDS |
TTAGGACTAAGTTAAAGTAA+CGG | 0.623009 | 2.2:-5117677 | None:intergenic |
GTGTGTTTAGGAGGAGCATG+TGG | 0.626776 | 2.2:+5116642 | MS.gene30973:intron |
AGATCAAAGTGAGGACGAGG+AGG | 0.633474 | 2.2:-5116882 | None:intergenic |
CTAGGTTGAGCAAAATTAGG+AGG | 0.633552 | 2.2:-5116837 | None:intergenic |
CAAACCGGCGAAAGGCGACA+GGG | 0.633653 | 2.2:-5116953 | None:intergenic |
AAAATTGCTGAATATCGCGC+CGG | 0.644009 | 2.2:+5116924 | MS.gene30973:CDS |
CACCACCATTGTAAACACCT+GGG | 0.648144 | 2.2:-5116426 | None:intergenic |
GTGTTTACAATGGTGGTGCA+TGG | 0.652108 | 2.2:+5116431 | MS.gene30973:CDS |
AGCACAGCACTATTCAACAA+TGG | 0.654867 | 2.2:+5116714 | MS.gene30973:CDS |
GTGCCATGTAGGAAGCGTGG+TGG | 0.655283 | 2.2:+5117630 | MS.gene30973:CDS |
AGAATCCCAGGTGTTTACAA+TGG | 0.660050 | 2.2:+5116421 | MS.gene30973:CDS |
GTTGCTGTGATGAAAATGGA+AGG | 0.660102 | 2.2:-5116804 | None:intergenic |
ATGTTAAGGGCACTAAAACA+GGG | 0.661896 | 2.2:+5117739 | MS.gene30973:CDS |
TTATTATGTCACTAACAATG+TGG | 0.670910 | 2.2:+5116386 | MS.gene30973:CDS |
CAGGTGCAGGGGATATAGTG+AGG | 0.673660 | 2.2:+5117712 | MS.gene30973:CDS |
ATGCCAATGAGCAGAAACTG+GGG | 0.677827 | 2.2:+5117765 | MS.gene30973:CDS |
GATCAAAGTGAGGACGAGGA+GGG | 0.681799 | 2.2:-5116881 | None:intergenic |
AGGGTGCCATGTAGGAAGCG+TGG | 0.699181 | 2.2:+5117627 | MS.gene30973:intron |
TGCCAATGAGCAGAAACTGG+GGG | 0.704067 | 2.2:+5117766 | MS.gene30973:CDS |
TTGCTGTGATGAAAATGGAA+GGG | 0.708603 | 2.2:-5116803 | None:intergenic |
GGCATGGCAAGATCAAAGTG+AGG | 0.710022 | 2.2:-5116891 | None:intergenic |
GCTCAACCTAGTGACAATGG+TGG | 0.714318 | 2.2:+5116849 | MS.gene30973:CDS |
AGTGTGCAAATGACAAGCAG+TGG | 0.728732 | 2.2:+5116766 | MS.gene30973:CDS |
TTAAGGGCACTAAAACAGGG+TGG | 0.761599 | 2.2:+5117742 | MS.gene30973:CDS |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|---|---|---|---|---|
!! | AAACTTAAAATAGTTTATGA+AGG | + | chr2.2:5117401-5117420 | MS.gene30973:intron | 15.0% |
!!! | AATAAGAAGTATATAGATAA+TGG | + | chr2.2:5117179-5117198 | MS.gene30973:intron | 15.0% |
!!! | ATAAGAAGTATATAGATAAT+GGG | + | chr2.2:5117180-5117199 | MS.gene30973:intron | 15.0% |
!!! | ATATATAAGCACTTTTATTA+GGG | - | chr2.2:5117287-5117306 | None:intergenic | 15.0% |
!!! | TATATATAAGCACTTTTATT+AGG | - | chr2.2:5117288-5117307 | None:intergenic | 15.0% |
!! | ATTAGTTATTTATTCCAACA+TGG | - | chr2.2:5117223-5117242 | None:intergenic | 20.0% |
!!! | TGCTCTATTAAAATTGATTT+TGG | + | chr2.2:5116997-5117016 | MS.gene30973:intron | 20.0% |
! | AGCAAATAAGCAAAAATGTA+GGG | - | chr2.2:5116981-5117000 | None:intergenic | 25.0% |
! | GAAAACATCTTATGACTTAT+AGG | - | chr2.2:5117352-5117371 | None:intergenic | 25.0% |
! | TTAGGACTAAGTTAAAGTAA+CGG | - | chr2.2:5117680-5117699 | None:intergenic | 25.0% |
! | TTATTATGTCACTAACAATG+TGG | + | chr2.2:5116386-5116405 | MS.gene30973:CDS | 25.0% |
!! | AAGTAAATAAGCCTTATCTA+AGG | - | chr2.2:5117520-5117539 | None:intergenic | 25.0% |
!! | GGATTGACATAATTTTTGTT+AGG | - | chr2.2:5117490-5117509 | None:intergenic | 25.0% |
!! | TTTGTTATGTTGATTATGCA+GGG | + | chr2.2:5117608-5117627 | MS.gene30973:intron | 25.0% |
!!! | TAGATAATGGGAGAATAAAT+TGG | + | chr2.2:5117192-5117211 | MS.gene30973:intron | 25.0% |
!!! | TTTTCTTAACTTCTTTGAAG+TGG | - | chr2.2:5117380-5117399 | None:intergenic | 25.0% |
!!! | TTTTGTTATGTTGATTATGC+AGG | + | chr2.2:5117607-5117626 | MS.gene30973:intron | 25.0% |
AAACAAACACTTGATGTCAT+CGG | - | chr2.2:5117031-5117050 | None:intergenic | 30.0% | |
AACTTGTATAGTCAAGGTTA+TGG | + | chr2.2:5116672-5116691 | MS.gene30973:CDS | 30.0% | |
ATAAATTGGTAAGACCATGT+TGG | + | chr2.2:5117206-5117225 | MS.gene30973:intron | 30.0% | |
GAGCAAATAAGCAAAAATGT+AGG | - | chr2.2:5116982-5117001 | None:intergenic | 30.0% | |
TATGGAAACTTGTATAGTCA+AGG | + | chr2.2:5116666-5116685 | MS.gene30973:CDS | 30.0% | |
TGTGATAGATCTCAATGATA+AGG | + | chr2.2:5117139-5117158 | MS.gene30973:intron | 30.0% | |
! | TTGGCAATCAAATTCAGTTT+TGG | + | chr2.2:5117794-5117813 | MS.gene30973:CDS | 30.0% |
!! | AATTTTAAGGAACATAGGCA+TGG | - | chr2.2:5116910-5116929 | None:intergenic | 30.0% |
!! | CAAGTGTTTGTTTGATTTTG+TGG | + | chr2.2:5117038-5117057 | MS.gene30973:intron | 30.0% |
!! | CAATCAAATTCAGTTTTGGT+GGG | + | chr2.2:5117798-5117817 | MS.gene30973:CDS | 30.0% |
!! | TCAGCAATTTTAAGGAACAT+AGG | - | chr2.2:5116915-5116934 | None:intergenic | 30.0% |
!!! | TTTGCCTTTTTTGTGTGTTT+AGG | + | chr2.2:5116630-5116649 | MS.gene30973:intron | 30.0% |
ATAGTGAGGACCTATGTTAA+GGG | + | chr2.2:5117726-5117745 | MS.gene30973:CDS | 35.0% | |
ATCCAAACATGCCTTAGATA+AGG | + | chr2.2:5117506-5117525 | MS.gene30973:intron | 35.0% | |
CAAAAATGTAGGGTACAAAC+CGG | - | chr2.2:5116971-5116990 | None:intergenic | 35.0% | |
CAATTTGGTCAAACTTTCAC+TGG | + | chr2.2:5117897-5117916 | MS.gene30973:CDS | 35.0% | |
GGGATTAGGTTCACAATAAA+CGG | + | chr2.2:5117651-5117670 | MS.gene30973:CDS | 35.0% | |
GTTAGTTGCTGTGATGAAAA+TGG | - | chr2.2:5116811-5116830 | None:intergenic | 35.0% | |
TATAGTGAGGACCTATGTTA+AGG | + | chr2.2:5117725-5117744 | MS.gene30973:CDS | 35.0% | |
TATGTCACTAACAATGTGGA+TGG | + | chr2.2:5116390-5116409 | MS.gene30973:CDS | 35.0% | |
TCCTCCTAAACACACAAAAA+AGG | - | chr2.2:5116637-5116656 | None:intergenic | 35.0% | |
TGACCAAATTGCCAATTATG+TGG | - | chr2.2:5117888-5117907 | None:intergenic | 35.0% | |
TTGCTGTGATGAAAATGGAA+GGG | - | chr2.2:5116806-5116825 | None:intergenic | 35.0% | |
! | CGCGATATTCAGCAATTTTA+AGG | - | chr2.2:5116923-5116942 | None:intergenic | 35.0% |
! | TCACTAGGTTGAGCAAAATT+AGG | - | chr2.2:5116843-5116862 | None:intergenic | 35.0% |
!! | ATGTTAAGGGCACTAAAACA+GGG | + | chr2.2:5117739-5117758 | MS.gene30973:CDS | 35.0% |
!! | GCAATCAAATTCAGTTTTGG+TGG | + | chr2.2:5117797-5117816 | MS.gene30973:CDS | 35.0% |
!! | TATGTTAAGGGCACTAAAAC+AGG | + | chr2.2:5117738-5117757 | MS.gene30973:CDS | 35.0% |
!! | TTCAACAATGGACTAAGTTG+TGG | + | chr2.2:5116726-5116745 | MS.gene30973:CDS | 35.0% |
!!! | TGTTTTAGTGCCCTTAACAT+AGG | - | chr2.2:5117739-5117758 | None:intergenic | 35.0% |
AAAATTGCTGAATATCGCGC+CGG | + | chr2.2:5116924-5116943 | MS.gene30973:CDS | 40.0% | |
AGAATCCCAGGTGTTTACAA+TGG | + | chr2.2:5116421-5116440 | MS.gene30973:CDS | 40.0% | |
AGCACAGCACTATTCAACAA+TGG | + | chr2.2:5116714-5116733 | MS.gene30973:CDS | 40.0% | |
ATGGTAAATGCTAGAATCCC+AGG | + | chr2.2:5116409-5116428 | MS.gene30973:CDS | 40.0% | |
GCTCCACATAATTGGCAATT+TGG | + | chr2.2:5117882-5117901 | MS.gene30973:CDS | 40.0% | |
GGAACATTGCTCCACATAAT+TGG | + | chr2.2:5117874-5117893 | MS.gene30973:CDS | 40.0% | |
GTTGCTGTGATGAAAATGGA+AGG | - | chr2.2:5116807-5116826 | None:intergenic | 40.0% | |
TTTAGTACTAGACGTACCCA+TGG | - | chr2.2:5116503-5116522 | None:intergenic | 40.0% | |
TTTGCTCAACCTAGTGACAA+TGG | + | chr2.2:5116846-5116865 | MS.gene30973:CDS | 40.0% | |
! | AGCCTTATCTAAGGCATGTT+TGG | - | chr2.2:5117511-5117530 | None:intergenic | 40.0% |
! | CTAGGTTGAGCAAAATTAGG+AGG | - | chr2.2:5116840-5116859 | None:intergenic | 40.0% |
!!! | ACAGCTCATGCCACTTTTTA+CGG | + | chr2.2:5116457-5116476 | MS.gene30973:CDS | 40.0% |
!!! | GCCTTTTTTGTGTGTTTAGG+AGG | + | chr2.2:5116633-5116652 | MS.gene30973:intron | 40.0% |
AACCTAGTGACAATGGTGGT+TGG | + | chr2.2:5116853-5116872 | MS.gene30973:CDS | 45.0% | |
AGTGTGCAAATGACAAGCAG+TGG | + | chr2.2:5116766-5116785 | MS.gene30973:CDS | 45.0% | |
ATCCCAGGTGTTTACAATGG+TGG | + | chr2.2:5116424-5116443 | MS.gene30973:CDS | 45.0% | |
ATCGGAACCTCCGTAAAAAG+TGG | - | chr2.2:5116470-5116489 | None:intergenic | 45.0% | |
ATGCCAATGAGCAGAAACTG+GGG | + | chr2.2:5117765-5117784 | MS.gene30973:CDS | 45.0% | |
CACCACCATTGTAAACACCT+GGG | - | chr2.2:5116429-5116448 | None:intergenic | 45.0% | |
GATGCCAATGAGCAGAAACT+GGG | + | chr2.2:5117764-5117783 | MS.gene30973:CDS | 45.0% | |
GCTTTGTCCTTCAGAGTTAC+CGG | + | chr2.2:5117825-5117844 | MS.gene30973:CDS | 45.0% | |
TGGAGCAATGTTCCAAGAAG+TGG | - | chr2.2:5117868-5117887 | None:intergenic | 45.0% | |
! | GTGTTTACAATGGTGGTGCA+TGG | + | chr2.2:5116431-5116450 | MS.gene30973:CDS | 45.0% |
! | TTAGGAGGAGCATGTGGATA+TGG | + | chr2.2:5116648-5116667 | MS.gene30973:intron | 45.0% |
!! | TTAAGGGCACTAAAACAGGG+TGG | + | chr2.2:5117742-5117761 | MS.gene30973:CDS | 45.0% |
AGATCAAAGTGAGGACGAGG+AGG | - | chr2.2:5116885-5116904 | None:intergenic | 50.0% | |
CACCAACCACCATTGTCACT+AGG | - | chr2.2:5116858-5116877 | None:intergenic | 50.0% | |
GATCAAAGTGAGGACGAGGA+GGG | - | chr2.2:5116884-5116903 | None:intergenic | 50.0% | |
GCAAGATCAAAGTGAGGACG+AGG | - | chr2.2:5116888-5116907 | None:intergenic | 50.0% | |
GCACCACCATTGTAAACACC+TGG | - | chr2.2:5116430-5116449 | None:intergenic | 50.0% | |
GCAGAAACTGGGGGCAAAAT+TGG | + | chr2.2:5117775-5117794 | MS.gene30973:CDS | 50.0% | |
GCTCAACCTAGTGACAATGG+TGG | + | chr2.2:5116849-5116868 | MS.gene30973:CDS | 50.0% | |
GGATGCCAATGAGCAGAAAC+TGG | + | chr2.2:5117763-5117782 | MS.gene30973:CDS | 50.0% | |
GGCATGGCAAGATCAAAGTG+AGG | - | chr2.2:5116894-5116913 | None:intergenic | 50.0% | |
GTCACTACCGGTAACTCTGA+AGG | - | chr2.2:5117835-5117854 | None:intergenic | 50.0% | |
GTCCTAATCAGCAACGTTGC+AGG | + | chr2.2:5117693-5117712 | MS.gene30973:CDS | 50.0% | |
TGCCAATGAGCAGAAACTGG+GGG | + | chr2.2:5117766-5117785 | MS.gene30973:CDS | 50.0% | |
! | CACCTGCAACGTTGCTGATT+AGG | - | chr2.2:5117698-5117717 | None:intergenic | 50.0% |
! | GATTATGCAGGGTGCCATGT+AGG | + | chr2.2:5117619-5117638 | MS.gene30973:intron | 50.0% |
! | GTGTGTTTAGGAGGAGCATG+TGG | + | chr2.2:5116642-5116661 | MS.gene30973:intron | 50.0% |
! | TCCAAGAAGTGGAAGTGCGA+CGG | - | chr2.2:5117857-5117876 | None:intergenic | 50.0% |
!! | GACAAGCAGTGGTGTCATTC+TGG | + | chr2.2:5116777-5116796 | MS.gene30973:CDS | 50.0% |
!!! | GCTCATGCCACTTTTTACGG+AGG | + | chr2.2:5116460-5116479 | MS.gene30973:CDS | 50.0% |
AAAGGCGACAGGGACGATTC+CGG | - | chr2.2:5116946-5116965 | None:intergenic | 55.0% | |
ACCGTCGCACTTCCACTTCT+TGG | + | chr2.2:5117853-5117872 | MS.gene30973:CDS | 55.0% | |
ACTAGACGTACCCATGGTGC+CGG | - | chr2.2:5116497-5116516 | None:intergenic | 55.0% | |
ATCAGCAACGTTGCAGGTGC+AGG | + | chr2.2:5117699-5117718 | MS.gene30973:CDS | 55.0% | |
ATCCCACCACGCTTCCTACA+TGG | - | chr2.2:5117636-5117655 | None:intergenic | 55.0% | |
CAGGTGCAGGGGATATAGTG+AGG | + | chr2.2:5117712-5117731 | MS.gene30973:CDS | 55.0% | |
GTAGGGTACAAACCGGCGAA+AGG | - | chr2.2:5116964-5116983 | None:intergenic | 55.0% | |
TACGGAGGTTCCGATGCTTC+CGG | + | chr2.2:5116475-5116494 | MS.gene30973:CDS | 55.0% | |
TCAGCAACGTTGCAGGTGCA+GGG | + | chr2.2:5117700-5117719 | MS.gene30973:CDS | 55.0% | |
TGCCCCCAGTTTCTGCTCAT+TGG | - | chr2.2:5117771-5117790 | None:intergenic | 55.0% | |
! | GTAGGAAGCGTGGTGGGATT+AGG | + | chr2.2:5117637-5117656 | MS.gene30973:CDS | 55.0% |
! | TGCCATGTAGGAAGCGTGGT+GGG | + | chr2.2:5117631-5117650 | MS.gene30973:CDS | 55.0% |
ACAAACCGGCGAAAGGCGAC+AGG | - | chr2.2:5116957-5116976 | None:intergenic | 60.0% | |
CAAACCGGCGAAAGGCGACA+GGG | - | chr2.2:5116956-5116975 | None:intergenic | 60.0% | |
CAGCAACGTTGCAGGTGCAG+GGG | + | chr2.2:5117701-5117720 | MS.gene30973:CDS | 60.0% | |
TCCGATGCTTCCGGCACCAT+GGG | + | chr2.2:5116484-5116503 | MS.gene30973:CDS | 60.0% | |
TTCCGATGCTTCCGGCACCA+TGG | + | chr2.2:5116483-5116502 | MS.gene30973:CDS | 60.0% | |
! | ACCCATGGTGCCGGAAGCAT+CGG | - | chr2.2:5116488-5116507 | None:intergenic | 60.0% |
! | AGGGTGCCATGTAGGAAGCG+TGG | + | chr2.2:5117627-5117646 | MS.gene30973:intron | 60.0% |
! | GGAAGTGCGACGGTCACTAC+CGG | - | chr2.2:5117847-5117866 | None:intergenic | 60.0% |
! | GTGCCATGTAGGAAGCGTGG+TGG | + | chr2.2:5117630-5117649 | MS.gene30973:CDS | 60.0% |
TCGTCCCTGTCGCCTTTCGC+CGG | + | chr2.2:5116949-5116968 | MS.gene30973:CDS | 65.0% |
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
chr2.2 | gene | 5116352 | 5117938 | 5116352 | ID=MS.gene30973 |
chr2.2 | mRNA | 5116352 | 5117938 | 5116352 | ID=MS.gene30973.t1;Parent=MS.gene30973 |
chr2.2 | exon | 5116352 | 5116505 | 5116352 | ID=MS.gene30973.t1.exon1;Parent=MS.gene30973.t1 |
chr2.2 | CDS | 5116352 | 5116505 | 5116352 | ID=cds.MS.gene30973.t1;Parent=MS.gene30973.t1 |
chr2.2 | exon | 5116652 | 5116970 | 5116652 | ID=MS.gene30973.t1.exon2;Parent=MS.gene30973.t1 |
chr2.2 | CDS | 5116652 | 5116970 | 5116652 | ID=cds.MS.gene30973.t1;Parent=MS.gene30973.t1 |
chr2.2 | exon | 5117629 | 5117938 | 5117629 | ID=MS.gene30973.t1.exon3;Parent=MS.gene30973.t1 |
chr2.2 | CDS | 5117629 | 5117938 | 5117629 | ID=cds.MS.gene30973.t1;Parent=MS.gene30973.t1 |
Gene Sequence |
Protein sequence |