AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31130.t1 MTR_2g097463 92.716 659 45 2 1 658 1 657 0.0 1249
MS.gene31130.t1 MTR_2g097467 81.335 659 119 3 1 658 1 656 0.0 1090
MS.gene31130.t1 MTR_2g097473 80.728 659 123 3 1 658 1 656 0.0 1089
MS.gene31130.t1 MTR_4g064200 57.722 641 205 12 38 658 58 652 0.0 691
MS.gene31130.t1 MTR_4g064160 43.004 679 325 13 33 658 17 686 1.84e-180 528
MS.gene31130.t1 MTR_7g062120 43.407 675 313 12 49 658 72 742 8.73e-180 529
MS.gene31130.t1 MTR_4g064120 45.628 629 282 14 54 658 36 628 6.68e-167 492
MS.gene31130.t1 MTR_4g064150 43.402 682 318 15 29 658 70 735 1.87e-166 494
MS.gene31130.t1 MTR_4g064180 45.151 629 285 14 54 658 36 628 3.29e-163 482
MS.gene31130.t1 MTR_2g097410 51.288 466 213 5 201 658 284 743 5.52e-162 483
MS.gene31130.t1 MTR_2g097390 45.958 433 221 5 231 658 219 643 4.45e-134 408
MS.gene31130.t1 MTR_2g097310 46.207 435 216 7 231 658 217 640 6.54e-134 407
MS.gene31130.t1 MTR_2g097350 45.287 435 221 6 231 658 218 642 7.24e-132 402
MS.gene31130.t1 MTR_2g097380 48.634 366 158 5 294 658 227 563 5.27e-121 372
MS.gene31130.t1 MTR_5g097480 31.903 373 248 5 287 655 173 543 2.34e-54 195
MS.gene31130.t1 MTR_2g026250 29.144 374 260 3 286 655 223 595 1.29e-53 194
MS.gene31130.t1 MTR_2g082090 31.903 373 248 5 287 655 208 578 1.53e-51 188
MS.gene31130.t1 MTR_4g133660 28.906 384 258 6 282 656 166 543 7.55e-51 185
MS.gene31130.t1 MTR_1g029420 27.989 368 261 2 291 655 225 591 2.59e-48 179
MS.gene31130.t1 MTR_7g027190 27.949 390 249 4 286 655 295 672 4.27e-48 180
MS.gene31130.t1 MTR_3g089055 29.111 371 253 6 291 655 198 564 1.71e-47 176
MS.gene31130.t1 MTR_3g089055 29.111 371 253 6 291 655 203 569 1.89e-47 176
MS.gene31130.t1 MTR_3g056110 29.067 375 256 5 287 655 171 541 4.26e-47 174
MS.gene31130.t1 MTR_8g442410 28.974 390 248 6 286 658 150 527 2.23e-46 172
MS.gene31130.t1 MTR_5g094450 29.551 379 253 8 287 655 157 531 7.72e-46 171
MS.gene31130.t1 MTR_4g104020 27.532 385 251 7 288 655 146 519 7.01e-45 168
MS.gene31130.t1 MTR_7g074650 29.620 368 240 7 294 657 441 793 6.01e-43 166
MS.gene31130.t1 MTR_3g065980 28.278 389 248 7 284 655 165 539 1.22e-41 159
MS.gene31130.t1 MTR_4g076140 25.995 427 248 6 291 655 48 468 4.65e-38 147
MS.gene31130.t1 MTR_4g102790 24.611 386 266 9 287 657 288 663 1.18e-37 149
MS.gene31130.t1 MTR_3g022830 26.859 417 245 7 292 655 26 435 1.65e-37 145
MS.gene31130.t1 MTR_2g034260 28.021 389 251 10 286 655 205 583 1.82e-37 147
MS.gene31130.t1 MTR_2g034250 27.411 394 248 10 286 655 205 584 4.55e-36 144
MS.gene31130.t1 MTR_4g076020 26.790 377 232 9 294 658 87 431 3.16e-33 133
MS.gene31130.t1 MTR_2g089100 27.861 402 248 11 291 658 52 445 7.99e-33 132
MS.gene31130.t1 MTR_4g077760 25.729 377 259 7 288 655 190 554 9.81e-33 133
MS.gene31130.t1 MTR_7g109580 27.461 386 248 11 286 655 184 553 2.51e-30 126
MS.gene31130.t1 MTR_4g097080 23.873 377 272 7 291 657 133 504 3.67e-30 125
MS.gene31130.t1 MTR_1g096030 26.768 396 248 11 288 655 77 458 1.56e-29 122
MS.gene31130.t1 MTR_2g034280 25.063 395 253 10 286 655 198 574 5.46e-29 122
MS.gene31130.t1 MTR_5g015490 25.197 381 268 7 291 657 114 491 9.19e-29 120
MS.gene31130.t1 MTR_1g069725 26.984 378 249 13 286 655 104 462 1.73e-28 119
MS.gene31130.t1 MTR_3g072710 28.205 312 197 9 357 656 204 500 4.66e-28 119
MS.gene31130.t1 MTR_1g086970 23.737 396 257 12 286 655 101 477 1.01e-24 108
MS.gene31130.t1 MTR_5g009080 30.622 209 135 3 291 495 56 258 1.66e-24 107
MS.gene31130.t1 MTR_5g009080 28.859 149 106 0 507 655 329 477 2.29e-16 82.8
MS.gene31130.t1 MTR_5g058860 24.791 359 240 11 318 656 149 497 1.86e-24 107
MS.gene31130.t1 MTR_7g057230 28.137 263 179 5 395 655 402 656 2.09e-24 108
MS.gene31130.t1 MTR_5g015950 22.482 427 277 10 281 658 27 448 4.51e-23 103
MS.gene31130.t1 MTR_3g053270 22.482 427 277 10 281 658 27 448 4.51e-23 103
MS.gene31130.t1 MTR_6g047750 26.715 277 185 6 388 655 394 661 8.82e-23 103
MS.gene31130.t1 MTR_4g095500 22.837 416 269 9 291 658 59 470 1.37e-21 99.0
MS.gene31130.t1 MTR_2g099110 25.000 392 256 11 291 657 74 452 6.37e-21 96.7
MS.gene31130.t1 MTR_8g093070 23.707 464 289 21 235 658 60 498 2.27e-20 95.5
MS.gene31130.t1 MTR_0092s0100 21.739 368 271 6 292 655 376 730 6.67e-14 75.5
MS.gene31130.t1 MTR_0092s0100 21.739 368 271 6 292 655 373 727 6.71e-14 75.5
MS.gene31130.t1 MTR_4g026485 19.950 401 286 12 269 655 244 623 2.18e-12 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31130.t1 AT2G29060 43.590 624 314 13 49 657 56 656 4.80e-165 488
MS.gene31130.t1 AT2G37650 41.667 660 326 12 42 657 58 702 6.65e-163 484
MS.gene31130.t1 AT2G37650 41.667 660 326 12 42 657 70 714 6.71e-163 485
MS.gene31130.t1 AT2G29060 42.373 649 323 13 49 657 56 693 1.18e-162 483
MS.gene31130.t1 AT1G07530 49.145 468 223 4 203 658 303 767 1.55e-160 480
MS.gene31130.t1 AT5G59450 45.336 536 255 8 154 658 72 600 1.23e-159 473
MS.gene31130.t1 AT3G46600 50.559 447 202 7 218 658 148 581 4.18e-148 442
MS.gene31130.t1 AT3G46600 50.559 447 202 7 218 658 116 549 4.90e-148 441
MS.gene31130.t1 AT2G29065 42.009 657 325 18 22 658 10 630 1.25e-147 442
MS.gene31130.t1 AT3G46600 54.839 372 162 2 291 658 4 373 6.48e-145 426
MS.gene31130.t1 AT1G07520 46.488 484 240 9 185 658 57 531 4.81e-137 412
MS.gene31130.t1 AT1G07520 46.488 484 240 9 185 658 57 531 4.81e-137 412
MS.gene31130.t1 AT1G07520 46.488 484 240 9 185 658 222 696 1.91e-135 413
MS.gene31130.t1 AT1G07520 46.488 484 240 9 185 658 221 695 2.11e-135 413
MS.gene31130.t1 AT1G50600 32.258 372 248 3 287 655 155 525 1.27e-53 192
MS.gene31130.t1 AT1G50600 32.258 372 248 3 287 655 226 596 5.06e-53 192
MS.gene31130.t1 AT1G50600 32.258 372 248 3 287 655 230 600 5.96e-53 192
MS.gene31130.t1 AT5G48150 29.843 382 263 3 278 655 109 489 3.67e-52 187
MS.gene31130.t1 AT5G48150 29.843 382 263 3 278 655 109 489 3.67e-52 187
MS.gene31130.t1 AT5G48150 29.843 382 263 3 278 655 109 489 3.67e-52 187
MS.gene31130.t1 AT5G48150 29.843 382 263 3 278 655 109 489 3.67e-52 187
MS.gene31130.t1 AT2G04890 29.570 372 251 4 287 655 49 412 1.65e-48 176
MS.gene31130.t1 AT4G17230 29.600 375 260 2 286 657 153 526 2.18e-46 172
MS.gene31130.t1 AT4G17230 29.600 375 260 2 286 657 153 526 2.18e-46 172
MS.gene31130.t1 AT1G21450 27.027 407 290 4 254 655 188 592 2.91e-45 170
MS.gene31130.t1 AT3G54220 31.283 374 229 11 294 657 296 651 2.93e-43 166
MS.gene31130.t1 AT3G03450 29.167 384 245 9 284 655 176 544 4.96e-42 160
MS.gene31130.t1 AT5G17490 27.126 435 292 9 229 655 98 515 8.87e-41 156
MS.gene31130.t1 AT1G66350 29.657 408 243 13 266 655 124 505 4.75e-40 154
MS.gene31130.t1 AT1G50420 24.885 434 251 6 288 655 54 478 6.08e-40 153
MS.gene31130.t1 AT1G14920 28.610 374 251 9 286 655 167 528 1.07e-39 153
MS.gene31130.t1 AT5G66770 27.346 373 260 7 288 655 217 583 3.55e-39 153
MS.gene31130.t1 AT5G41920 26.546 388 251 8 281 655 33 399 2.75e-36 141
MS.gene31130.t1 AT2G01570 27.005 374 257 9 286 655 219 580 2.90e-36 144
MS.gene31130.t1 AT1G63100 26.786 392 255 9 287 656 248 629 2.43e-33 136
MS.gene31130.t1 AT1G63100 26.786 392 255 9 287 656 273 654 2.72e-33 136
MS.gene31130.t1 AT3G50650 25.352 355 251 5 309 655 193 541 4.19e-33 134
MS.gene31130.t1 AT4G37650 25.255 392 263 8 290 657 145 530 1.05e-32 133
MS.gene31130.t1 AT4G08250 28.650 363 236 12 307 656 125 477 3.18e-31 128
MS.gene31130.t1 AT3G49950 26.562 384 261 9 291 657 30 409 8.96e-29 119
MS.gene31130.t1 AT5G52510 25.326 383 255 11 292 655 269 639 2.73e-28 120
MS.gene31130.t1 AT1G55580 24.031 387 228 10 320 655 73 444 1.34e-23 104
MS.gene31130.t1 AT3G13840 22.402 433 281 18 253 655 102 509 5.80e-15 78.6
MS.gene31130.t1 AT4G00150 21.839 435 285 13 235 655 160 553 9.34e-11 65.5

Find 137 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTGATCCAGATGGAAATTT+TGG 0.205044 2.4:-6331301 MS.gene31130:CDS
CATCGACTACAAAATCTTTA+TGG 0.219672 2.4:+6329733 None:intergenic
CCTGACCTGCCATTGCTTGT+AGG 0.253220 2.4:+6329836 None:intergenic
TCCAAGTACCATTCTGAAAA+TGG 0.263904 2.4:+6331092 None:intergenic
AGTGTTCATATTATTGATTT+TGG 0.276562 2.4:-6330440 MS.gene31130:CDS
AGGCAACACTCTTCGCCTTT+TGG 0.276975 2.4:-6330671 MS.gene31130:CDS
TATGTATCTGAGGATGTTAT+TGG 0.308200 2.4:+6331465 None:intergenic
TTCCTTCTTCACCATTGCTT+TGG 0.317965 2.4:+6329758 None:intergenic
AGCTTCCTCAGCCAGGATTT+AGG 0.323032 2.4:-6330328 MS.gene31130:CDS
CACTAAGCTGTAAATTGTAT+TGG 0.330543 2.4:+6330475 None:intergenic
GTATCATCATATGATGAATT+AGG 0.332225 2.4:+6331325 None:intergenic
TTTGTATGCTTTATCTGCTT+GGG 0.337273 2.4:-6329670 MS.gene31130:CDS
GTTACATGGTTCAATGAGTT+AGG 0.343395 2.4:+6331574 None:intergenic
CCCGTAATTCGAAGCTTTGC+AGG 0.346583 2.4:+6330356 None:intergenic
CGAATTTGACTCTTCCTACT+TGG 0.356470 2.4:-6331197 MS.gene31130:CDS
GTTTCCTCTGCCCTTTCTGC+AGG 0.358323 2.4:+6330305 None:intergenic
ACAACTGCATTGCACAGAAA+TGG 0.365582 2.4:-6330232 MS.gene31130:CDS
GCATCACATAATGTTGATTC+AGG 0.366614 2.4:-6330845 MS.gene31130:CDS
CTCTCAAGCATCAGCCTTTG+CGG 0.367574 2.4:+6329930 None:intergenic
TTGGATTAATCTTCCTGATC+AGG 0.372735 2.4:+6330081 None:intergenic
TTAGCTCATTACTTTGCTAA+TGG 0.378914 2.4:-6330632 MS.gene31130:CDS
GCAGAAAGGGCAGAGGAAAC+TGG 0.381946 2.4:-6330302 MS.gene31130:CDS
TCCTGCAAAGCTTCGAATTA+CGG 0.385651 2.4:-6330357 MS.gene31130:CDS
TCTGATTCGGTTAACCTTGT+TGG 0.387152 2.4:-6331220 MS.gene31130:CDS
CCTGCAAAGCTTCGAATTAC+GGG 0.388387 2.4:-6330356 MS.gene31130:CDS
TCTCAAGCATCAGCCTTTGC+GGG 0.391520 2.4:+6329931 None:intergenic
TCATTGAACCATGTAACAAA+TGG 0.394328 2.4:-6331568 MS.gene31130:CDS
GCGCAAACCCTGCATGTTGC+AGG 0.405053 2.4:-6331407 MS.gene31130:CDS
GAGCTAGGTTCAAGCAAATC+AGG 0.411714 2.4:-6329806 MS.gene31130:CDS
TTCTTCCATGGAGTTGTCAA+TGG 0.415847 2.4:-6330053 MS.gene31130:CDS
ATGCTTGAGAGAGGGTTGTT+TGG 0.419092 2.4:-6329918 MS.gene31130:CDS
TGCCATTGCTTGTAGGTCTC+AGG 0.423545 2.4:+6329843 None:intergenic
CAACATTATGTGATGCTTGT+TGG 0.426130 2.4:+6330852 None:intergenic
TTAGTAGAGCAAAACTACTA+TGG 0.426979 2.4:-6331160 MS.gene31130:CDS
AAATCTTGCAGAAAGGTGTT+AGG 0.427722 2.4:+6331604 None:intergenic
CATTGAACCATGTAACAAAT+GGG 0.436405 2.4:-6331567 MS.gene31130:CDS
ATGAGCTACTCAAGCAGATT+AGG 0.437525 2.4:-6330691 MS.gene31130:CDS
ATCGTTGTTTGATATGTTTG+AGG 0.437931 2.4:-6329973 MS.gene31130:CDS
CACAGAAATGGGAAACTATC+CGG 0.438062 2.4:-6330220 MS.gene31130:CDS
CAACTGCATTGCACAGAAAT+GGG 0.438307 2.4:-6330231 MS.gene31130:CDS
CCTACAAGCAATGGCAGGTC+AGG 0.440693 2.4:-6329836 MS.gene31130:CDS
AAGCATTGTTGAACCTGATC+AGG 0.443595 2.4:-6330094 MS.gene31130:CDS
AGGATTTAGGCCTGCAGAAA+GGG 0.451557 2.4:-6330315 MS.gene31130:CDS
TTCAGAATGGTACTTGGAGT+TGG 0.458122 2.4:-6331087 MS.gene31130:CDS
AGGTTCAACAATGCTTCTCT+TGG 0.460604 2.4:+6330101 None:intergenic
CTTTCTGCAGGCCTAAATCC+TGG 0.464941 2.4:+6330317 None:intergenic
ACTCAAACCCATTTGTTACA+TGG 0.465056 2.4:+6331560 None:intergenic
CATGGATGAAGAAGATGACT+TGG 0.465816 2.4:-6331431 MS.gene31130:CDS
GTGCTTCCCTAAACCGAGTC+AGG 0.469223 2.4:+6330009 None:intergenic
TTGAGAGAGGGTTGTTTGGA+AGG 0.472118 2.4:-6329914 MS.gene31130:CDS
AACTCAATGTGCACAGGCCG+TGG 0.475370 2.4:-6330738 MS.gene31130:CDS
TTCACTGCTACAGTTTCATC+AGG 0.476635 2.4:+6330128 None:intergenic
TAAACATTCCTGCAACATGC+AGG 0.476644 2.4:+6331399 None:intergenic
GGAATATCTATTCCCTGTGT+AGG 0.479623 2.4:+6331486 None:intergenic
TAGTCGATGTCGATGGTAAG+TGG 0.481602 2.4:-6329719 MS.gene31130:CDS
ATGCCACAGTTGTGGATTTG+TGG 0.483676 2.4:-6330769 MS.gene31130:CDS
CTGCTACAGTTTCATCAGGC+AGG 0.484383 2.4:+6330132 None:intergenic
TAATTCATGTGAGTCTGATT+CGG 0.485201 2.4:-6331233 MS.gene31130:CDS
CGCTGAAGTCCATCCCCAAA+AGG 0.487864 2.4:+6330656 None:intergenic
TACTTCTTTGTCAGCAAGGA+AGG 0.488696 2.4:-6330889 MS.gene31130:CDS
AGCAATGGCAGGTCAGGAAC+AGG 0.489352 2.4:-6329830 MS.gene31130:CDS
TGCTCAATGAGCACTGGATC+AGG 0.490813 2.4:+6331127 None:intergenic
AGTACCTCATCTAAGATCTC+TGG 0.492137 2.4:+6330908 None:intergenic
CGAAGCTTTGCAGGACCACC+AGG 0.497005 2.4:+6330365 None:intergenic
CCGCTTGATGTAGTAACTGC+TGG 0.498718 2.4:-6330569 MS.gene31130:CDS
AACCAAAGCAATGGTGAAGA+AGG 0.500696 2.4:-6329760 MS.gene31130:CDS
CGGCATATACGATGGAATCT+CGG 0.504421 2.4:+6330589 None:intergenic
AGCTTGTGAGGGTGCGGAGA+GGG 0.504460 2.4:-6329874 MS.gene31130:CDS
GTGCGGCCAAAATTTCCATC+TGG 0.507021 2.4:+6331295 None:intergenic
GGAATTGAGCTTCCTCAGCC+AGG 0.513281 2.4:-6330335 MS.gene31130:CDS
CAGGATTTAGGCCTGCAGAA+AGG 0.516377 2.4:-6330316 MS.gene31130:CDS
AGGTCAGGAACAGGAGAGCT+AGG 0.516508 2.4:-6329821 MS.gene31130:CDS
TCGAGGATCTAAAGATAGAC+AGG 0.520782 2.4:-6330196 MS.gene31130:CDS
AGCCTTTGCGGGTCTTCGCA+AGG 0.523484 2.4:+6329942 None:intergenic
AGGAGTTTGTAGTTCCAAGT+AGG 0.525291 2.4:+6331183 None:intergenic
GCTTGTTGCTCAATGAGCAC+TGG 0.529649 2.4:+6331121 None:intergenic
TGTAACAAATGGGTTTGAGT+TGG 0.530549 2.4:-6331557 MS.gene31130:CDS
GAAACCTCTTCAACCATCAC+TGG 0.530989 2.4:+6331052 None:intergenic
TCTGAGCTTATGACAGAAGA+AGG 0.531044 2.4:+6331517 None:intergenic
CCTTTGATCGTAGCTTGCCA+CGG 0.533325 2.4:+6330721 None:intergenic
CCGTGGCAAGCTACGATCAA+AGG 0.538969 2.4:-6330721 MS.gene31130:CDS
TGAGAGAGGGTTGTTTGGAA+GGG 0.539295 2.4:-6329913 MS.gene31130:CDS
GAATATCTATTCCCTGTGTA+GGG 0.540588 2.4:+6331487 None:intergenic
AACACTAAAATCTTGCAGAA+AGG 0.548523 2.4:+6331597 None:intergenic
TACAAACAATGCCACAGTTG+TGG 0.553896 2.4:-6330777 MS.gene31130:CDS
TCAGCCAGTGATGGTTGAAG+AGG 0.554665 2.4:-6331056 MS.gene31130:CDS
TAATGTTGATTCAGGAGGAA+AGG 0.556753 2.4:-6330837 MS.gene31130:CDS
GAATTTGCTATGGTTTCCAG+TGG 0.556961 2.4:-6330418 MS.gene31130:CDS
AGAAAAGAGAAGTCATGTGA+TGG 0.557380 2.4:-6331011 MS.gene31130:CDS
GCAATGCAGTTGTATTCAAA+TGG 0.559815 2.4:+6330242 None:intergenic
TGCCTTGCGAAGACCCGCAA+AGG 0.561465 2.4:-6329944 MS.gene31130:CDS
ATACATAAGCGACATCCTCA+TGG 0.562884 2.4:-6331449 MS.gene31130:CDS
TGTTATAGCTTGTGAGGGTG+CGG 0.569313 2.4:-6329880 MS.gene31130:CDS
GAATCAAATTCAGCCAGTGA+TGG 0.572156 2.4:-6331065 MS.gene31130:CDS
AAACATTCCTGCAACATGCA+GGG 0.581348 2.4:+6331400 None:intergenic
AACTTCCATTGACAACTCCA+TGG 0.582257 2.4:+6330048 None:intergenic
TATCAAACAACGATGAGAAG+TGG 0.582630 2.4:+6329982 None:intergenic
TGAGACCTACAAGCAATGGC+AGG 0.588661 2.4:-6329841 MS.gene31130:CDS
GGCCTGAGACCTACAAGCAA+TGG 0.588665 2.4:-6329845 MS.gene31130:CDS
TGATACTAGTAATGAACAAG+AGG 0.594503 2.4:-6330978 MS.gene31130:CDS
ATATTCGTCATTCTCTGCAA+AGG 0.596904 2.4:+6330506 None:intergenic
TATAAATGTTATAGCTTGTG+AGG 0.597621 2.4:-6329886 MS.gene31130:CDS
CAAAGGCTGATGCTTGAGAG+AGG 0.599769 2.4:-6329927 MS.gene31130:CDS
TCTTGCAGAAAGGTGTTAGG+AGG 0.601548 2.4:+6331607 None:intergenic
AGTCGATGTCGATGGTAAGT+GGG 0.601905 2.4:-6329718 MS.gene31130:CDS
GATGATACCACTGATCCAGA+TGG 0.602284 2.4:-6331310 MS.gene31130:CDS
AAGAGTCAAATTCGCCAACA+AGG 0.602536 2.4:+6331206 None:intergenic
AGTGAATGAAACCAAAGCAA+TGG 0.607650 2.4:-6329769 MS.gene31130:CDS
TCAGATGAAGATCCCTACAC+AGG 0.608008 2.4:-6331499 MS.gene31130:CDS
AGGGTGCGGAGAGGGTCGAA+AGG 0.608738 2.4:-6329866 MS.gene31130:CDS
CTATCTTTAGATCCTCGAGC+CGG 0.610122 2.4:+6330201 None:intergenic
GAGGTACTTCTTTGTCAGCA+AGG 0.610885 2.4:-6330893 MS.gene31130:CDS
TATGCTAACTCAATGTGCAC+AGG 0.610937 2.4:-6330744 MS.gene31130:CDS
TAGGTTCAAGCAAATCAGGT+TGG 0.612445 2.4:-6329802 MS.gene31130:CDS
CATTCTCTGCAAAGGAGATG+AGG 0.613851 2.4:+6330514 None:intergenic
ACCTCATCTAAGATCTCTGG+TGG 0.614764 2.4:+6330911 None:intergenic
GCAGGCCTAAATCCTGGCTG+AGG 0.614834 2.4:+6330323 None:intergenic
CCAGCAGTTACTACATCAAG+CGG 0.615147 2.4:+6330569 None:intergenic
TAGGCCTGCAGAAAGGGCAG+AGG 0.619921 2.4:-6330309 MS.gene31130:CDS
AAAATTTCCATCTGGATCAG+TGG 0.623541 2.4:+6331303 None:intergenic
AAAGGCTGATGCTTGAGAGA+GGG 0.635382 2.4:-6329926 MS.gene31130:CDS
ATGGGAAACTATCCGGCTCG+AGG 0.640423 2.4:-6330213 MS.gene31130:CDS
TCACATAATGTTGATTCAGG+AGG 0.642164 2.4:-6330842 MS.gene31130:CDS
CAGATGAAGATCCCTACACA+GGG 0.642715 2.4:-6331498 MS.gene31130:CDS
TAGCTTGTGAGGGTGCGGAG+AGG 0.646388 2.4:-6329875 MS.gene31130:CDS
ACATCAAGCGGCATATACGA+TGG 0.651925 2.4:+6330581 None:intergenic
TGATTCAGGAGGAAAGGCAA+AGG 0.662454 2.4:-6330831 MS.gene31130:CDS
GATACTAGTAATGAACAAGA+GGG 0.667890 2.4:-6330977 MS.gene31130:CDS
ATAAATGTTATAGCTTGTGA+GGG 0.673348 2.4:-6329885 MS.gene31130:CDS
AAACTTTCAATGAGACCTGG+TGG 0.675722 2.4:-6330380 MS.gene31130:CDS
AGACTCACATGAATTATCAG+TGG 0.680494 2.4:+6331240 None:intergenic
AAAGGGCAGAGGAAACTGGA+AGG 0.686895 2.4:-6330298 MS.gene31130:CDS
AGTCATCTTCTTCATCCATG+AGG 0.688746 2.4:+6331434 None:intergenic
ACCACCAGAGATCTTAGATG+AGG 0.690503 2.4:-6330912 MS.gene31130:CDS
GGATGTCGCTTATGTATCTG+AGG 0.698731 2.4:+6331455 None:intergenic
AGTCCACAAATCCACAACTG+TGG 0.729336 2.4:+6330766 None:intergenic
TGCTCTCAAAACTAGCAGTG+CGG 0.748557 2.4:+6331278 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTGTTCATATTATTGATTT+TGG - chr2.4:6330832-6330851 MS.gene31130:CDS 20.0%
! GTATCATCATATGATGAATT+AGG + chr2.4:6329950-6329969 None:intergenic 25.0%
!! ATAAATGTTATAGCTTGTGA+GGG - chr2.4:6331387-6331406 MS.gene31130:CDS 25.0%
!! TATAAATGTTATAGCTTGTG+AGG - chr2.4:6331386-6331405 MS.gene31130:CDS 25.0%
!!! ATTGATTTTGGAATTTGCTA+TGG - chr2.4:6330844-6330863 MS.gene31130:CDS 25.0%
!!! TCATTGAAAGTTTTTTGATG+AGG + chr2.4:6330885-6330904 None:intergenic 25.0%
AAAAAACTTTCAATGAGACC+TGG - chr2.4:6330889-6330908 MS.gene31130:CDS 30.0%
AACACTAAAATCTTGCAGAA+AGG + chr2.4:6329678-6329697 None:intergenic 30.0%
AATCCAAAAATCTTCTTCCA+TGG - chr2.4:6331207-6331226 MS.gene31130:CDS 30.0%
ATCGTTGTTTGATATGTTTG+AGG - chr2.4:6331299-6331318 MS.gene31130:CDS 30.0%
CACTAAGCTGTAAATTGTAT+TGG + chr2.4:6330800-6330819 None:intergenic 30.0%
CATTGAACCATGTAACAAAT+GGG - chr2.4:6329705-6329724 MS.gene31130:CDS 30.0%
GATACTAGTAATGAACAAGA+GGG - chr2.4:6330295-6330314 MS.gene31130:CDS 30.0%
TAATTCATGTGAGTCTGATT+CGG - chr2.4:6330039-6330058 MS.gene31130:CDS 30.0%
TATGTATCTGAGGATGTTAT+TGG + chr2.4:6329810-6329829 None:intergenic 30.0%
TCATTGAACCATGTAACAAA+TGG - chr2.4:6329704-6329723 MS.gene31130:CDS 30.0%
TGATACTAGTAATGAACAAG+AGG - chr2.4:6330294-6330313 MS.gene31130:CDS 30.0%
TTAGCTCATTACTTTGCTAA+TGG - chr2.4:6330640-6330659 MS.gene31130:CDS 30.0%
TTAGTAGAGCAAAACTACTA+TGG - chr2.4:6330112-6330131 MS.gene31130:CDS 30.0%
TTTGTATGCTTTATCTGCTT+GGG - chr2.4:6331602-6331621 MS.gene31130:CDS 30.0%
! CATCGACTACAAAATCTTTA+TGG + chr2.4:6331542-6331561 None:intergenic 30.0%
! TTTTCTCTTGGTACTAATGA+AGG + chr2.4:6330247-6330266 None:intergenic 30.0%
! TTTTGTATGCTTTATCTGCT+TGG - chr2.4:6331601-6331620 MS.gene31130:CDS 30.0%
!! TAGTAGTTTTGCTCTACTAA+AGG + chr2.4:6330112-6330131 None:intergenic 30.0%
AAAATTTCCATCTGGATCAG+TGG + chr2.4:6329972-6329991 None:intergenic 35.0%
AGAAAAGAGAAGTCATGTGA+TGG - chr2.4:6330261-6330280 MS.gene31130:CDS 35.0%
AGACTCACATGAATTATCAG+TGG + chr2.4:6330035-6330054 None:intergenic 35.0%
AGTGAATGAAACCAAAGCAA+TGG - chr2.4:6331503-6331522 MS.gene31130:CDS 35.0%
ATATTCGTCATTCTCTGCAA+AGG + chr2.4:6330769-6330788 None:intergenic 35.0%
CAACATTATGTGATGCTTGT+TGG + chr2.4:6330423-6330442 None:intergenic 35.0%
GAATATCTATTCCCTGTGTA+GGG + chr2.4:6329788-6329807 None:intergenic 35.0%
GCAATGCAGTTGTATTCAAA+TGG + chr2.4:6331033-6331052 None:intergenic 35.0%
GTTACATGGTTCAATGAGTT+AGG + chr2.4:6329701-6329720 None:intergenic 35.0%
TATCAAACAACGATGAGAAG+TGG + chr2.4:6331293-6331312 None:intergenic 35.0%
TTGGATTAATCTTCCTGATC+AGG + chr2.4:6331194-6331213 None:intergenic 35.0%
! ACATGACTTCTCTTTTCTCT+TGG + chr2.4:6330259-6330278 None:intergenic 35.0%
! ACCATTTTCAGAATGGTACT+TGG - chr2.4:6330179-6330198 MS.gene31130:CDS 35.0%
! ACTCAAACCCATTTGTTACA+TGG + chr2.4:6329715-6329734 None:intergenic 35.0%
! ACTCCATGGAAGAAGATTTT+TGG + chr2.4:6331213-6331232 None:intergenic 35.0%
! ACTGATCCAGATGGAAATTT+TGG - chr2.4:6329971-6329990 MS.gene31130:CDS 35.0%
! TCCAAGTACCATTCTGAAAA+TGG + chr2.4:6330183-6330202 None:intergenic 35.0%
! TGTAACAAATGGGTTTGAGT+TGG - chr2.4:6329715-6329734 MS.gene31130:CDS 35.0%
!! AAATCTTGCAGAAAGGTGTT+AGG + chr2.4:6329671-6329690 None:intergenic 35.0%
!! AGAATGATTTTTCAGCAGCT+TGG + chr2.4:6329904-6329923 None:intergenic 35.0%
!! GCATCACATAATGTTGATTC+AGG - chr2.4:6330427-6330446 MS.gene31130:CDS 35.0%
!! TAATGTTGATTCAGGAGGAA+AGG - chr2.4:6330435-6330454 MS.gene31130:CDS 35.0%
!! TCACATAATGTTGATTCAGG+AGG - chr2.4:6330430-6330449 MS.gene31130:CDS 35.0%
!!! GAAAGTTTTTTGATGAGGCA+TGG + chr2.4:6330880-6330899 None:intergenic 35.0%
AAACATTCCTGCAACATGCA+GGG + chr2.4:6329875-6329894 None:intergenic 40.0%
AAACTTTCAATGAGACCTGG+TGG - chr2.4:6330892-6330911 MS.gene31130:CDS 40.0%
AACCAAAGCAATGGTGAAGA+AGG - chr2.4:6331512-6331531 MS.gene31130:CDS 40.0%
AACTTCCATTGACAACTCCA+TGG + chr2.4:6331227-6331246 None:intergenic 40.0%
AAGAGTCAAATTCGCCAACA+AGG + chr2.4:6330069-6330088 None:intergenic 40.0%
ACAACTGCATTGCACAGAAA+TGG - chr2.4:6331040-6331059 MS.gene31130:CDS 40.0%
AGGAGTTTGTAGTTCCAAGT+AGG + chr2.4:6330092-6330111 None:intergenic 40.0%
AGGTTCAACAATGCTTCTCT+TGG + chr2.4:6331174-6331193 None:intergenic 40.0%
AGTCATCTTCTTCATCCATG+AGG + chr2.4:6329841-6329860 None:intergenic 40.0%
ATACATAAGCGACATCCTCA+TGG - chr2.4:6329823-6329842 MS.gene31130:CDS 40.0%
ATGAGCTACTCAAGCAGATT+AGG - chr2.4:6330581-6330600 MS.gene31130:CDS 40.0%
CAACTGCATTGCACAGAAAT+GGG - chr2.4:6331041-6331060 MS.gene31130:CDS 40.0%
CACAGAAATGGGAAACTATC+CGG - chr2.4:6331052-6331071 MS.gene31130:CDS 40.0%
CATGGATGAAGAAGATGACT+TGG - chr2.4:6329841-6329860 MS.gene31130:CDS 40.0%
CGAATTTGACTCTTCCTACT+TGG - chr2.4:6330075-6330094 MS.gene31130:CDS 40.0%
CTAAACCGAGTCAGGAAAAA+CGG + chr2.4:6331258-6331277 None:intergenic 40.0%
GAATCAAATTCAGCCAGTGA+TGG - chr2.4:6330207-6330226 MS.gene31130:CDS 40.0%
GGAATATCTATTCCCTGTGT+AGG + chr2.4:6329789-6329808 None:intergenic 40.0%
TAAACATTCCTGCAACATGC+AGG + chr2.4:6329876-6329895 None:intergenic 40.0%
TACAAACAATGCCACAGTTG+TGG - chr2.4:6330495-6330514 MS.gene31130:CDS 40.0%
TACTTCTTTGTCAGCAAGGA+AGG - chr2.4:6330383-6330402 MS.gene31130:CDS 40.0%
TAGGTTCAAGCAAATCAGGT+TGG - chr2.4:6331470-6331489 MS.gene31130:CDS 40.0%
TATGCTAACTCAATGTGCAC+AGG - chr2.4:6330528-6330547 MS.gene31130:CDS 40.0%
TCCTGCAAAGCTTCGAATTA+CGG - chr2.4:6330915-6330934 MS.gene31130:CDS 40.0%
TCGAGGATCTAAAGATAGAC+AGG - chr2.4:6331076-6331095 MS.gene31130:CDS 40.0%
TCTGAGCTTATGACAGAAGA+AGG + chr2.4:6329758-6329777 None:intergenic 40.0%
TTCACTGCTACAGTTTCATC+AGG + chr2.4:6331147-6331166 None:intergenic 40.0%
TTCAGAATGGTACTTGGAGT+TGG - chr2.4:6330185-6330204 MS.gene31130:CDS 40.0%
TTCCTTCTTCACCATTGCTT+TGG + chr2.4:6331517-6331536 None:intergenic 40.0%
TTCTTCCATGGAGTTGTCAA+TGG - chr2.4:6331219-6331238 MS.gene31130:CDS 40.0%
! AAGCATTGTTGAACCTGATC+AGG - chr2.4:6331178-6331197 MS.gene31130:CDS 40.0%
! AGTACCTCATCTAAGATCTC+TGG + chr2.4:6330367-6330386 None:intergenic 40.0%
! GATTTTGTAGTCGATGTCGA+TGG - chr2.4:6331546-6331565 MS.gene31130:CDS 40.0%
! GCTGCTAACCATTTTCAGAA+TGG - chr2.4:6330172-6330191 MS.gene31130:CDS 40.0%
!! GAATTTGCTATGGTTTCCAG+TGG - chr2.4:6330854-6330873 MS.gene31130:CDS 40.0%
!! TCTGATTCGGTTAACCTTGT+TGG - chr2.4:6330052-6330071 MS.gene31130:CDS 40.0%
!!! ACTGCTAGTTTTGAGAGCAA+TGG - chr2.4:6329998-6330017 MS.gene31130:CDS 40.0%
!!! GTTTTTCCTGACTCGGTTTA+GGG - chr2.4:6331257-6331276 MS.gene31130:CDS 40.0%
AAAGGCTGATGCTTGAGAGA+GGG - chr2.4:6331346-6331365 MS.gene31130:CDS 45.0%
ACATCAAGCGGCATATACGA+TGG + chr2.4:6330694-6330713 None:intergenic 45.0%
ACCACCAGAGATCTTAGATG+AGG - chr2.4:6330360-6330379 MS.gene31130:CDS 45.0%
AGGATTTAGGCCTGCAGAAA+GGG - chr2.4:6330957-6330976 MS.gene31130:CDS 45.0%
AGTCCACAAATCCACAACTG+TGG + chr2.4:6330509-6330528 None:intergenic 45.0%
AGTCGATGTCGATGGTAAGT+GGG - chr2.4:6331554-6331573 MS.gene31130:CDS 45.0%
ATGCCACAGTTGTGGATTTG+TGG - chr2.4:6330503-6330522 MS.gene31130:CDS 45.0%
ATGCTTGAGAGAGGGTTGTT+TGG - chr2.4:6331354-6331373 MS.gene31130:CDS 45.0%
CAGATGAAGATCCCTACACA+GGG - chr2.4:6329774-6329793 MS.gene31130:CDS 45.0%
CATTCTCTGCAAAGGAGATG+AGG + chr2.4:6330761-6330780 None:intergenic 45.0%
CCAGCAGTTACTACATCAAG+CGG + chr2.4:6330706-6330725 None:intergenic 45.0%
CCTGCAAAGCTTCGAATTAC+GGG - chr2.4:6330916-6330935 MS.gene31130:CDS 45.0%
CGGCATATACGATGGAATCT+CGG + chr2.4:6330686-6330705 None:intergenic 45.0%
CTATCTTTAGATCCTCGAGC+CGG + chr2.4:6331074-6331093 None:intergenic 45.0%
GAAACCTCTTCAACCATCAC+TGG + chr2.4:6330223-6330242 None:intergenic 45.0%
GAGCTAGGTTCAAGCAAATC+AGG - chr2.4:6331466-6331485 MS.gene31130:CDS 45.0%
GATGATACCACTGATCCAGA+TGG - chr2.4:6329962-6329981 MS.gene31130:CDS 45.0%
TAGTCGATGTCGATGGTAAG+TGG - chr2.4:6331553-6331572 MS.gene31130:CDS 45.0%
TCAGATGAAGATCCCTACAC+AGG - chr2.4:6329773-6329792 MS.gene31130:CDS 45.0%
TGAGAGAGGGTTGTTTGGAA+GGG - chr2.4:6331359-6331378 MS.gene31130:CDS 45.0%
TGATTCAGGAGGAAAGGCAA+AGG - chr2.4:6330441-6330460 MS.gene31130:CDS 45.0%
TGCTCTCAAAACTAGCAGTG+CGG + chr2.4:6329997-6330016 None:intergenic 45.0%
TTGAGAGAGGGTTGTTTGGA+AGG - chr2.4:6331358-6331377 MS.gene31130:CDS 45.0%
! ACCTCATCTAAGATCTCTGG+TGG + chr2.4:6330364-6330383 None:intergenic 45.0%
! GAGGTACTTCTTTGTCAGCA+AGG - chr2.4:6330379-6330398 MS.gene31130:CDS 45.0%
! GGATGTCGCTTATGTATCTG+AGG + chr2.4:6329820-6329839 None:intergenic 45.0%
! TGTTATAGCTTGTGAGGGTG+CGG - chr2.4:6331392-6331411 MS.gene31130:CDS 45.0%
!! GATGGTAAGTGGGTTTTGCA+GGG - chr2.4:6331564-6331583 MS.gene31130:CDS 45.0%
!! TCTTGCAGAAAGGTGTTAGG+AGG + chr2.4:6329668-6329687 None:intergenic 45.0%
!!! CGTTTTTCCTGACTCGGTTT+AGG - chr2.4:6331256-6331275 MS.gene31130:CDS 45.0%
AAAGGGCAGAGGAAACTGGA+AGG - chr2.4:6330974-6330993 MS.gene31130:CDS 50.0%
AGCTTCCTCAGCCAGGATTT+AGG - chr2.4:6330944-6330963 MS.gene31130:CDS 50.0%
CAAAGGCTGATGCTTGAGAG+AGG - chr2.4:6331345-6331364 MS.gene31130:CDS 50.0%
CAGGATTTAGGCCTGCAGAA+AGG - chr2.4:6330956-6330975 MS.gene31130:CDS 50.0%
CCCGTAATTCGAAGCTTTGC+AGG + chr2.4:6330919-6330938 None:intergenic 50.0%
CCGCTTGATGTAGTAACTGC+TGG - chr2.4:6330703-6330722 MS.gene31130:CDS 50.0%
CTCTCAAGCATCAGCCTTTG+CGG + chr2.4:6331345-6331364 None:intergenic 50.0%
CTGCTACAGTTTCATCAGGC+AGG + chr2.4:6331143-6331162 None:intergenic 50.0%
CTTTCTGCAGGCCTAAATCC+TGG + chr2.4:6330958-6330977 None:intergenic 50.0%
GTGCGGCCAAAATTTCCATC+TGG + chr2.4:6329980-6329999 None:intergenic 50.0%
TCAGCCAGTGATGGTTGAAG+AGG - chr2.4:6330216-6330235 MS.gene31130:CDS 50.0%
TCTCAAGCATCAGCCTTTGC+GGG + chr2.4:6331344-6331363 None:intergenic 50.0%
TGAGACCTACAAGCAATGGC+AGG - chr2.4:6331431-6331450 MS.gene31130:CDS 50.0%
TGCCATTGCTTGTAGGTCTC+AGG + chr2.4:6331432-6331451 None:intergenic 50.0%
! AGGCAACACTCTTCGCCTTT+TGG - chr2.4:6330601-6330620 MS.gene31130:CDS 50.0%
! CCTTTGATCGTAGCTTGCCA+CGG + chr2.4:6330554-6330573 None:intergenic 50.0%
! GCAACACTCTTCGCCTTTTG+GGG - chr2.4:6330603-6330622 MS.gene31130:CDS 50.0%
! GCTTGTTGCTCAATGAGCAC+TGG + chr2.4:6330154-6330173 None:intergenic 50.0%
! GGCAACACTCTTCGCCTTTT+GGG - chr2.4:6330602-6330621 MS.gene31130:CDS 50.0%
! TGGGTTTTGCAGGGATGGAA+AGG - chr2.4:6331573-6331592 MS.gene31130:CDS 50.0%
! TTTTGATGAGGCATGGCCAC+TGG + chr2.4:6330873-6330892 None:intergenic 50.0%
!! CGATGGTAAGTGGGTTTTGC+AGG - chr2.4:6331563-6331582 MS.gene31130:CDS 50.0%
!! CTTTTGGGGATGGACTTCAG+CGG - chr2.4:6330617-6330636 MS.gene31130:CDS 50.0%
!! GTAAGTGGGTTTTGCAGGGA+TGG - chr2.4:6331568-6331587 MS.gene31130:CDS 50.0%
!! GTGCACCGTTTTTCCTGACT+CGG - chr2.4:6331250-6331269 MS.gene31130:CDS 50.0%
!! TGCTCAATGAGCACTGGATC+AGG + chr2.4:6330148-6330167 None:intergenic 50.0%
AACTCAATGTGCACAGGCCG+TGG - chr2.4:6330534-6330553 MS.gene31130:CDS 55.0%
AGCAATGGCAGGTCAGGAAC+AGG - chr2.4:6331442-6331461 MS.gene31130:CDS 55.0%
AGGTCAGGAACAGGAGAGCT+AGG - chr2.4:6331451-6331470 MS.gene31130:CDS 55.0%
CCGTGGCAAGCTACGATCAA+AGG - chr2.4:6330551-6330570 MS.gene31130:CDS 55.0%
CCTACAAGCAATGGCAGGTC+AGG - chr2.4:6331436-6331455 MS.gene31130:CDS 55.0%
CCTGACCTGCCATTGCTTGT+AGG + chr2.4:6331439-6331458 None:intergenic 55.0%
CGCTGAAGTCCATCCCCAAA+AGG + chr2.4:6330619-6330638 None:intergenic 55.0%
GCAGAAAGGGCAGAGGAAAC+TGG - chr2.4:6330970-6330989 MS.gene31130:CDS 55.0%
GGAATTGAGCTTCCTCAGCC+AGG - chr2.4:6330937-6330956 MS.gene31130:CDS 55.0%
GGCCTGAGACCTACAAGCAA+TGG - chr2.4:6331427-6331446 MS.gene31130:CDS 55.0%
GTGCTTCCCTAAACCGAGTC+AGG + chr2.4:6331266-6331285 None:intergenic 55.0%
GTTTCCTCTGCCCTTTCTGC+AGG + chr2.4:6330970-6330989 None:intergenic 55.0%
! ATGGGAAACTATCCGGCTCG+AGG - chr2.4:6331059-6331078 MS.gene31130:CDS 55.0%
!! CACTCTTCGCCTTTTGGGGA+TGG - chr2.4:6330607-6330626 MS.gene31130:CDS 55.0%
AGCCTTTGCGGGTCTTCGCA+AGG + chr2.4:6331333-6331352 None:intergenic 60.0%
CGAAGCTTTGCAGGACCACC+AGG + chr2.4:6330910-6330929 None:intergenic 60.0%
GCAGGCCTAAATCCTGGCTG+AGG + chr2.4:6330952-6330971 None:intergenic 60.0%
GCGCAAACCCTGCATGTTGC+AGG - chr2.4:6329865-6329884 MS.gene31130:CDS 60.0%
TAGGCCTGCAGAAAGGGCAG+AGG - chr2.4:6330963-6330982 MS.gene31130:CDS 60.0%
TGCCTTGCGAAGACCCGCAA+AGG - chr2.4:6331328-6331347 MS.gene31130:CDS 60.0%
! AGCTTGTGAGGGTGCGGAGA+GGG - chr2.4:6331398-6331417 MS.gene31130:CDS 60.0%
! TAGCTTGTGAGGGTGCGGAG+AGG - chr2.4:6331397-6331416 MS.gene31130:CDS 60.0%
AGGGTGCGGAGAGGGTCGAA+AGG - chr2.4:6331406-6331425 MS.gene31130:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 6329659 6331635 6329659 ID=MS.gene31130
chr2.4 mRNA 6329659 6331635 6329659 ID=MS.gene31130.t1;Parent=MS.gene31130
chr2.4 exon 6329659 6331635 6329659 ID=MS.gene31130.t1.exon1;Parent=MS.gene31130.t1
chr2.4 CDS 6329659 6331635 6329659 ID=cds.MS.gene31130.t1;Parent=MS.gene31130.t1
Gene Sequence

>MS.gene31130

ATGCTTCCTCCTAACACCTTTCTGCAAGATTTTAGTGTTCCTAACTCATTGAACCATGTAACAAATGGGTTTGAGTTGGAAGATTCATCATTTTCACCTTCTTCTGTCATAAGCTCAGATGAAGATCCCTACACAGGGAATAGATATTCCAATAACATCCTCAGATACATAAGCGACATCCTCATGGATGAAGAAGATGACTTGGAGCGCAAACCCTGCATGTTGCAGGAATGTTTAAAACTCCAAGCTGCTGAAAAATCATTCTATGATGTTCTTAGTCATAACAATCCTAATTCATCATATGATGATACCACTGATCCAGATGGAAATTTTGGCCGCACTGCTAGTTTTGAGAGCAATGGCAGCAGCTGCACCACTGATAATTCATGTGAGTCTGATTCGGTTAACCTTGTTGGCGAATTTGACTCTTCCTACTTGGAACTACAAACTCCTTTAGTAGAGCAAAACTACTATGGTGTGACACAACCTGATCCAGTGCTCATTGAGCAACAAGCTGCTAACCATTTTCAGAATGGTACTTGGAGTTGGAATCAAATTCAGCCAGTGATGGTTGAAGAGGTTTCACCTTCATTAGTACCAAGAGAAAAGAGAAGTCATGTGATGGATGATAACGATGATACTAGTAATGAACAAGAGGGAAGAGATAGCAAGATTTCAGCTATCTTTTTTGATGAATTAGAACCACCAGAGATCTTAGATGAGGTACTTCTTTGTCAGCAAGGAAGGACACTCACTCAACACCAACAAGCATCACATAATGTTGATTCAGGAGGAAAGGCAAAGGCAACACGTTCTCGATCGAAGAAAGTATCTACTACAAACAATGCCACAGTTGTGGATTTGTGGACTATGCTAACTCAATGTGCACAGGCCGTGGCAAGCTACGATCAAAGGAATACAAATGAGCTACTCAAGCAGATTAGGCAACACTCTTCGCCTTTTGGGGATGGACTTCAGCGGTTAGCTCATTACTTTGCTAATGGTCTTGAGATAAGATTTGCTGCCGAGATTCCATCGTATATGCCGCTTGATGTAGTAACTGCTGGAGATATGTTGAAAGCTTACAAACTATTCGTTACCTCATCTCCTTTGCAGAGAATGACGAATATGTTGTTAACCAATACAATTTACAGCTTAGTGAAGAACGAGAGCAGTGTTCATATTATTGATTTTGGAATTTGCTATGGTTTCCAGTGGCCATGCCTCATCAAAAAACTTTCAATGAGACCTGGTGGTCCTGCAAAGCTTCGAATTACGGGAATTGAGCTTCCTCAGCCAGGATTTAGGCCTGCAGAAAGGGCAGAGGAAACTGGAAGGCGTTTAGAGAACTACTGCAAGAAGTTCAATGTACCATTTGAATACAACTGCATTGCACAGAAATGGGAAACTATCCGGCTCGAGGATCTAAAGATAGACAGGAATGAAGTAACTCTTGTTAGCTGTTTATACAGAATGAAGAACCTGCCTGATGAAACTGTAGCAGTGAATTGTCCAAGAGAAGCATTGTTGAACCTGATCAGGAAGATTAATCCAAAAATCTTCTTCCATGGAGTTGTCAATGGAAGTTATAGTGCACCGTTTTTCCTGACTCGGTTTAGGGAAGCACTCTACCACTTCTCATCGTTGTTTGATATGTTTGAGGCTAACGTGCCTTGCGAAGACCCGCAAAGGCTGATGCTTGAGAGAGGGTTGTTTGGAAGGGATGCTATAAATGTTATAGCTTGTGAGGGTGCGGAGAGGGTCGAAAGGCCTGAGACCTACAAGCAATGGCAGGTCAGGAACAGGAGAGCTAGGTTCAAGCAAATCAGGTTGGATTTTGATTTAGTGAATGAAACCAAAGCAATGGTGAAGAAGGAATACCATAAAGATTTTGTAGTCGATGTCGATGGTAAGTGGGTTTTGCAGGGATGGAAAGGACGCATTTTGTATGCTTTATCTGCTTGGGTTCCTGCTTAA
Protein sequence

>MS.gene31130.t1

MLPPNTFLQDFSVPNSLNHVTNGFELEDSSFSPSSVISSDEDPYTGNRYSNNILRYISDILMDEEDDLERKPCMLQECLKLQAAEKSFYDVLSHNNPNSSYDDTTDPDGNFGRTASFESNGSSCTTDNSCESDSVNLVGEFDSSYLELQTPLVEQNYYGVTQPDPVLIEQQAANHFQNGTWSWNQIQPVMVEEVSPSLVPREKRSHVMDDNDDTSNEQEGRDSKISAIFFDELEPPEILDEVLLCQQGRTLTQHQQASHNVDSGGKAKATRSRSKKVSTTNNATVVDLWTMLTQCAQAVASYDQRNTNELLKQIRQHSSPFGDGLQRLAHYFANGLEIRFAAEIPSYMPLDVVTAGDMLKAYKLFVTSSPLQRMTNMLLTNTIYSLVKNESSVHIIDFGICYGFQWPCLIKKLSMRPGGPAKLRITGIELPQPGFRPAERAEETGRRLENYCKKFNVPFEYNCIAQKWETIRLEDLKIDRNEVTLVSCLYRMKNLPDETVAVNCPREALLNLIRKINPKIFFHGVVNGSYSAPFFLTRFREALYHFSSLFDMFEANVPCEDPQRLMLERGLFGRDAINVIACEGAERVERPETYKQWQVRNRRARFKQIRLDFDLVNETKAMVKKEYHKDFVVDVDGKWVLQGWKGRILYALSAWVPA