AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31421


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31421.t1 MTR_2g090735 99.190 247 1 1 1 247 40 285 0.0 507
MS.gene31421.t1 MTR_4g078875 77.689 251 47 4 1 247 39 284 3.63e-136 384
MS.gene31421.t1 MTR_4g088245 67.200 250 72 4 2 246 60 304 4.73e-115 332
MS.gene31421.t1 MTR_2g014680 61.753 251 89 3 2 247 45 293 2.93e-107 312
MS.gene31421.t1 MTR_8g069160 84.146 164 26 0 2 165 60 223 2.73e-100 293
MS.gene31421.t1 MTR_8g102240 77.966 177 32 2 9 178 46 222 1.68e-93 283
MS.gene31421.t1 MTR_8g102240 77.966 177 32 2 9 178 46 222 1.73e-93 283
MS.gene31421.t1 MTR_5g053430 58.659 179 73 1 4 182 52 229 4.06e-67 214
MS.gene31421.t1 MTR_5g053430 58.659 179 73 1 4 182 52 229 5.37e-67 214
MS.gene31421.t1 MTR_1g093680 59.302 172 69 1 5 176 53 223 1.45e-66 214
MS.gene31421.t1 MTR_7g033320 57.576 165 69 1 6 169 51 215 6.50e-61 199
MS.gene31421.t1 MTR_1g093670 51.163 172 69 1 5 176 18 174 9.53e-50 166
MS.gene31421.t1 MTR_4g078895 82.353 51 9 0 1 51 28 78 5.92e-24 92.0
MS.gene31421.t1 MTR_1g008740 31.841 201 106 9 10 201 13 191 7.53e-16 76.3
MS.gene31421.t1 MTR_1g008740 31.841 201 106 9 10 201 27 205 1.09e-15 75.9
MS.gene31421.t1 MTR_3g070030 32.203 177 89 8 10 176 19 174 2.07e-14 72.0
MS.gene31421.t1 MTR_7g085260 32.995 197 95 9 10 188 17 194 3.79e-14 71.6
MS.gene31421.t1 MTR_6g084430 32.934 167 89 6 9 170 4 152 4.08e-14 70.9
MS.gene31421.t1 MTR_3g109340 32.530 166 86 7 10 167 22 169 6.81e-14 70.5
MS.gene31421.t1 MTR_3g435150 31.694 183 96 7 10 181 16 180 1.58e-13 68.9
MS.gene31421.t1 MTR_7g011120 29.348 184 97 6 10 179 8 172 3.99e-13 68.2
MS.gene31421.t1 MTR_2g014700 65.116 43 15 0 75 117 44 86 5.15e-13 63.5
MS.gene31421.t1 MTR_4g108760 31.579 171 88 7 10 170 17 168 8.01e-13 67.4
MS.gene31421.t1 MTR_2g080010 30.667 150 85 5 8 154 13 146 1.37e-11 63.9
MS.gene31421.t1 MTR_1g069805 28.313 166 96 6 9 169 9 156 2.83e-11 62.4
MS.gene31421.t1 MTR_3g096920 33.562 146 75 7 10 152 7 133 5.01e-11 62.0
MS.gene31421.t1 MTR_8g094580 32.192 146 77 6 10 152 7 133 5.36e-11 61.6
MS.gene31421.t1 MTR_3g096920 33.562 146 75 7 10 152 7 133 5.78e-11 62.0
MS.gene31421.t1 MTR_3g088110 32.639 144 79 6 10 152 7 133 7.22e-11 61.2
MS.gene31421.t1 MTR_2g078700 30.952 168 89 7 10 167 23 173 7.31e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31421.t1 AT4G28500 81.622 185 33 1 2 185 51 235 5.48e-111 322
MS.gene31421.t1 AT1G28470 78.857 175 37 0 2 176 69 243 4.49e-100 294
MS.gene31421.t1 AT4G29230 76.966 178 33 1 9 178 47 224 1.94e-94 284
MS.gene31421.t1 AT4G29230 76.966 178 33 1 9 178 46 223 8.11e-94 284
MS.gene31421.t1 AT4G29230 76.966 178 33 1 9 178 47 224 8.52e-94 284
MS.gene31421.t1 AT5G56620 72.472 178 38 3 9 175 42 219 6.82e-89 269
MS.gene31421.t1 AT5G56620 72.472 178 38 3 9 175 42 219 7.32e-89 268
MS.gene31421.t1 AT5G56620 72.472 178 38 3 9 175 42 219 1.28e-88 267
MS.gene31421.t1 AT1G25580 59.064 171 63 3 4 174 52 215 1.09e-62 202
MS.gene31421.t1 AT5G14490 54.762 168 73 3 8 174 53 218 9.98e-54 175
MS.gene31421.t1 AT5G14490 54.762 168 73 3 8 174 53 218 1.54e-53 176
MS.gene31421.t1 AT5G14490 54.762 168 73 3 8 174 56 221 1.79e-53 176
MS.gene31421.t1 AT5G14490 54.762 168 73 3 8 174 56 221 2.28e-53 176
MS.gene31421.t1 AT3G01600 53.939 165 74 2 8 171 53 216 3.42e-51 171
MS.gene31421.t1 AT3G04060 32.961 179 96 6 2 172 12 174 6.42e-16 76.3
MS.gene31421.t1 AT5G18270 34.637 179 91 7 3 172 14 175 8.67e-16 75.9
MS.gene31421.t1 AT5G18270 34.637 179 91 7 3 172 14 175 1.57e-15 75.1
MS.gene31421.t1 AT3G18400 34.731 167 81 8 9 167 4 150 1.24e-14 72.4
MS.gene31421.t1 AT3G29035 32.941 170 83 8 10 168 24 173 1.37e-13 69.3
MS.gene31421.t1 AT5G07680 32.143 168 91 5 10 170 17 168 2.00e-13 68.9
MS.gene31421.t1 AT5G07680 32.143 168 91 5 10 170 3 154 2.24e-13 68.9
MS.gene31421.t1 AT5G39610 33.929 168 82 7 10 167 20 168 3.94e-13 67.8
MS.gene31421.t1 AT5G61430 31.953 169 90 6 10 170 16 167 6.20e-13 67.8
MS.gene31421.t1 AT1G01720 31.333 150 85 5 4 152 1 133 1.10e-12 66.6
MS.gene31421.t1 AT1G02230 32.877 146 73 7 11 149 4 131 1.03e-11 64.3
MS.gene31421.t1 AT5G08790 32.877 146 76 6 10 152 7 133 1.04e-11 63.9
MS.gene31421.t1 AT1G76420 31.844 179 92 9 9 177 21 179 1.30e-11 63.9
MS.gene31421.t1 AT4G35580 29.082 196 106 10 3 187 2 175 2.02e-11 63.9
MS.gene31421.t1 AT4G35580 29.082 196 106 10 3 187 2 175 2.06e-11 63.9
MS.gene31421.t1 AT4G35580 29.082 196 106 10 3 187 2 175 2.44e-11 63.5
MS.gene31421.t1 AT1G33060 28.090 178 97 9 3 169 17 174 3.77e-11 63.2
MS.gene31421.t1 AT1G33060 28.090 178 97 9 3 169 17 174 3.83e-11 63.2
MS.gene31421.t1 AT1G02220 31.690 142 77 6 11 149 4 128 6.23e-11 62.0
MS.gene31421.t1 AT5G14000 31.250 160 92 8 10 167 15 158 9.25e-11 60.1

Find 48 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTTGCCACTCACGAAAAC+TGG 0.294833 2.4:+9699535 None:intergenic
TCAAGAGATTCTTGAACATT+TGG 0.312906 2.4:-9699967 MS.gene31421:CDS
GAAAGCCAGAGAAAACAAAT+TGG 0.314159 2.4:-9699462 MS.gene31421:CDS
CACATTGTCTAGGCTGTGTT+TGG 0.340883 2.4:+9699365 None:intergenic
AGAGGAGAAAGAAGGAGAAT+TGG 0.354646 2.4:-9699407 MS.gene31421:CDS
TTCGTGAGTGGCAAGTTAAA+AGG 0.359924 2.4:-9699529 MS.gene31421:CDS
AATTGACCTGGTAACTTCTC+TGG 0.367159 2.4:+9699849 None:intergenic
CATTATTTCCAAGATGGTAT+TGG 0.373788 2.4:+9699431 None:intergenic
TATGCAGGTGTCGGCAAAGA+TGG 0.386728 2.4:-9699682 MS.gene31421:intron
GAGTTACCTGGATTACCTGC+TGG 0.403541 2.4:-9700011 MS.gene31421:CDS
GAAACAAGATGGCATAAAAC+AGG 0.423381 2.4:-9699568 MS.gene31421:CDS
GGGATGAACTCATCAATTAA+AGG 0.427373 2.4:+9699909 None:intergenic
CCTCTTCATTATTTCCAAGA+TGG 0.434430 2.4:+9699425 None:intergenic
GAGTTCATCCCTACTCTTGA+AGG 0.437508 2.4:-9699897 MS.gene31421:CDS
CTGTGTTTGGTAGAAAACTT+TGG 0.443831 2.4:+9699378 None:intergenic
CCTACTCTTGAAGGAGAGAA+TGG 0.452976 2.4:-9699888 MS.gene31421:CDS
AGAGATTCTTGAACATTTGG+AGG 0.472757 2.4:-9699964 MS.gene31421:CDS
CCATCGAAAGCATACACAAC+AGG 0.476509 2.4:-9699634 MS.gene31421:CDS
TTCACCCCAGCAGGTAATCC+AGG 0.479471 2.4:+9700005 None:intergenic
CCATTCTCTCCTTCAAGAGT+AGG 0.481831 2.4:+9699888 None:intergenic
GGATCAAACTTCACCCCAGC+AGG 0.493343 2.4:+9699996 None:intergenic
GTTATGCACCAATACCATCT+TGG 0.493624 2.4:-9699439 MS.gene31421:CDS
TGATCCGACATTTCTTTCAC+AGG 0.494146 2.4:-9699657 MS.gene31421:CDS
CATTCTCTCCTTCAAGAGTA+GGG 0.494352 2.4:+9699889 None:intergenic
AGTTACCTGGATTACCTGCT+GGG 0.500946 2.4:-9700010 MS.gene31421:CDS
AAAGCCAGAGAAAACAAATT+GGG 0.504516 2.4:-9699461 MS.gene31421:CDS
GTTAATGAATTATAGGGTGC+TGG 0.506242 2.4:-9700041 None:intergenic
CCTGTTGTGTATGCTTTCGA+TGG 0.507588 2.4:+9699634 None:intergenic
AATAATGAAGAGGAGAAAGA+AGG 0.526318 2.4:-9699415 MS.gene31421:CDS
ATGCAGGTGTCGGCAAAGAT+GGG 0.533540 2.4:-9699681 MS.gene31421:intron
TCAAGAATCTCTTGATCAGT+AGG 0.542994 2.4:+9699975 None:intergenic
GCTGGAATTCATGAGTTACC+TGG 0.547070 2.4:-9700023 MS.gene31421:CDS
AAAGATGTACCATCATGGAC+AGG 0.570055 2.4:+9699157 None:intergenic
GAATGAAAGATGTACCATCA+TGG 0.578897 2.4:+9699152 None:intergenic
ACTCATCCAGAGAAGTTACC+AGG 0.581252 2.4:-9699855 MS.gene31421:intron
AATGAACCACCACATTGTCT+AGG 0.596526 2.4:+9699355 None:intergenic
TGTAGTTAGTGTAAAGCACA+AGG 0.598633 2.4:+9699494 None:intergenic
CAGATGGAAGTGAAACAAGA+TGG 0.608219 2.4:-9699579 MS.gene31421:CDS
ATGGCCTGTGAAAGAAATGT+CGG 0.608865 2.4:+9699653 None:intergenic
AACATTTGGAGGCAAAAGTG+AGG 0.622163 2.4:-9699953 MS.gene31421:CDS
CCATCTTGGAAATAATGAAG+AGG 0.636615 2.4:-9699425 MS.gene31421:CDS
AAAGTGCACACAGATGCAGA+TGG 0.660294 2.4:-9699595 MS.gene31421:CDS
GGCATAAAACAGGTAAAACA+AGG 0.665333 2.4:-9699558 MS.gene31421:CDS
CAAACACAGCCTAGACAATG+TGG 0.666262 2.4:-9699364 MS.gene31421:CDS
ACACAGCCTAGACAATGTGG+TGG 0.735435 2.4:-9699361 MS.gene31421:CDS
TCAAGGTGTGAATCATGAGG+TGG 0.742861 2.4:-9699287 MS.gene31421:CDS
TGATCAAGGTGTGAATCATG+AGG 0.746754 2.4:-9699290 MS.gene31421:CDS
GTTACCTGGATTACCTGCTG+GGG 0.752999 2.4:-9700009 MS.gene31421:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATATATATATTTGG+TGG + chr2.4:9699354-9699373 None:intergenic 10.0%
!! GGCATATATATATATATATT+TGG + chr2.4:9699357-9699376 None:intergenic 15.0%
!!! TTTTCTGATAAAAAACTAAA+TGG - chr2.4:9699860-9699879 MS.gene31421:CDS 15.0%
!! CTATGAAAGAATAAAAGAAA+TGG + chr2.4:9699394-9699413 None:intergenic 20.0%
!! GAAATGGAAAGTAAATTATA+AGG + chr2.4:9699378-9699397 None:intergenic 20.0%
! AAAAAACTAAATGGTGATCA+AGG - chr2.4:9699869-9699888 MS.gene31421:CDS 25.0%
! CACAAAAACTGTCATTATAT+AGG + chr2.4:9699446-9699465 None:intergenic 25.0%
!! ATATTTTACGTCTAATATGC+AGG - chr2.4:9699476-9699495 MS.gene31421:CDS 25.0%
!! TCTTTCATATCTTTTGATCA+AGG - chr2.4:9699950-9699969 MS.gene31421:CDS 25.0%
AAAGCCAGAGAAAACAAATT+GGG - chr2.4:9699712-9699731 MS.gene31421:intron 30.0%
AATAATGAAGAGGAGAAAGA+AGG - chr2.4:9699758-9699777 MS.gene31421:intron 30.0%
CATTATTTCCAAGATGGTAT+TGG + chr2.4:9699745-9699764 None:intergenic 30.0%
GTGGTTAATCATAAAAACAG+TGG - chr2.4:9699905-9699924 MS.gene31421:CDS 30.0%
TCAAGAGATTCTTGAACATT+TGG - chr2.4:9699206-9699225 MS.gene31421:CDS 30.0%
!! ATAACCCAATTTGTTTTCTC+TGG + chr2.4:9699719-9699738 None:intergenic 30.0%
AGAGATTCTTGAACATTTGG+AGG - chr2.4:9699209-9699228 MS.gene31421:CDS 35.0%
CCATCTTGGAAATAATGAAG+AGG - chr2.4:9699748-9699767 MS.gene31421:intron 35.0%
CCTCTTCATTATTTCCAAGA+TGG + chr2.4:9699751-9699770 None:intergenic 35.0%
CTGTGTTTGGTAGAAAACTT+TGG + chr2.4:9699798-9699817 None:intergenic 35.0%
GAAACAAGATGGCATAAAAC+AGG - chr2.4:9699605-9699624 MS.gene31421:CDS 35.0%
GAAAGCCAGAGAAAACAAAT+TGG - chr2.4:9699711-9699730 MS.gene31421:intron 35.0%
GAATGAAAGATGTACCATCA+TGG + chr2.4:9700024-9700043 None:intergenic 35.0%
GGCATAAAACAGGTAAAACA+AGG - chr2.4:9699615-9699634 MS.gene31421:CDS 35.0%
GGGATGAACTCATCAATTAA+AGG + chr2.4:9699267-9699286 None:intergenic 35.0%
TCAAGAATCTCTTGATCAGT+AGG + chr2.4:9699201-9699220 None:intergenic 35.0%
TGTAGTTAGTGTAAAGCACA+AGG + chr2.4:9699682-9699701 None:intergenic 35.0%
AAAGATGTACCATCATGGAC+AGG + chr2.4:9700019-9700038 None:intergenic 40.0%
AACATTTGGAGGCAAAAGTG+AGG - chr2.4:9699220-9699239 MS.gene31421:CDS 40.0%
AATGAACCACCACATTGTCT+AGG + chr2.4:9699821-9699840 None:intergenic 40.0%
AATTGACCTGGTAACTTCTC+TGG + chr2.4:9699327-9699346 None:intergenic 40.0%
AGAGGAGAAAGAAGGAGAAT+TGG - chr2.4:9699766-9699785 MS.gene31421:intron 40.0%
ATGGCCTGTGAAAGAAATGT+CGG + chr2.4:9699523-9699542 None:intergenic 40.0%
CAGATGGAAGTGAAACAAGA+TGG - chr2.4:9699594-9699613 MS.gene31421:CDS 40.0%
CATTCTCTCCTTCAAGAGTA+GGG + chr2.4:9699287-9699306 None:intergenic 40.0%
GGTACATCTTTCATTCCTTG+AGG - chr2.4:9700028-9700047 MS.gene31421:CDS 40.0%
GTTATGCACCAATACCATCT+TGG - chr2.4:9699734-9699753 MS.gene31421:intron 40.0%
TACGTCTAATATGCAGGTGT+CGG - chr2.4:9699482-9699501 MS.gene31421:CDS 40.0%
TGATCAAGGTGTGAATCATG+AGG - chr2.4:9699883-9699902 MS.gene31421:CDS 40.0%
TGATCCGACATTTCTTTCAC+AGG - chr2.4:9699516-9699535 MS.gene31421:CDS 40.0%
TTCGTGAGTGGCAAGTTAAA+AGG - chr2.4:9699644-9699663 MS.gene31421:CDS 40.0%
! TCTCATTTTCCTGTCCATGA+TGG - chr2.4:9700007-9700026 MS.gene31421:CDS 40.0%
! TTTGGTGGCTTAAATTGACC+TGG + chr2.4:9699339-9699358 None:intergenic 40.0%
AAAGTGCACACAGATGCAGA+TGG - chr2.4:9699578-9699597 MS.gene31421:CDS 45.0%
AACTTGCCACTCACGAAAAC+TGG + chr2.4:9699641-9699660 None:intergenic 45.0%
ACTCATCCAGAGAAGTTACC+AGG - chr2.4:9699318-9699337 MS.gene31421:CDS 45.0%
AGTTACCTGGATTACCTGCT+GGG - chr2.4:9699163-9699182 MS.gene31421:CDS 45.0%
CAAACACAGCCTAGACAATG+TGG - chr2.4:9699809-9699828 MS.gene31421:intron 45.0%
CACATTGTCTAGGCTGTGTT+TGG + chr2.4:9699811-9699830 None:intergenic 45.0%
CCATTCTCTCCTTCAAGAGT+AGG + chr2.4:9699288-9699307 None:intergenic 45.0%
CCTACTCTTGAAGGAGAGAA+TGG - chr2.4:9699285-9699304 MS.gene31421:CDS 45.0%
CCTGTTGTGTATGCTTTCGA+TGG + chr2.4:9699542-9699561 None:intergenic 45.0%
GAGTTCATCCCTACTCTTGA+AGG - chr2.4:9699276-9699295 MS.gene31421:CDS 45.0%
GCTGGAATTCATGAGTTACC+TGG - chr2.4:9699150-9699169 MS.gene31421:CDS 45.0%
TCAAGGTGTGAATCATGAGG+TGG - chr2.4:9699886-9699905 MS.gene31421:CDS 45.0%
! CCATCGAAAGCATACACAAC+AGG - chr2.4:9699539-9699558 MS.gene31421:CDS 45.0%
ACACAGCCTAGACAATGTGG+TGG - chr2.4:9699812-9699831 MS.gene31421:intron 50.0%
GAGTTACCTGGATTACCTGC+TGG - chr2.4:9699162-9699181 MS.gene31421:CDS 50.0%
GTTACCTGGATTACCTGCTG+GGG - chr2.4:9699164-9699183 MS.gene31421:CDS 50.0%
! ACAAGGCCAGTTTTCGTGAG+TGG - chr2.4:9699632-9699651 MS.gene31421:CDS 50.0%
!! ATGCAGGTGTCGGCAAAGAT+GGG - chr2.4:9699492-9699511 MS.gene31421:CDS 50.0%
!! TATGCAGGTGTCGGCAAAGA+TGG - chr2.4:9699491-9699510 MS.gene31421:CDS 50.0%
GGATCAAACTTCACCCCAGC+AGG + chr2.4:9699180-9699199 None:intergenic 55.0%
TTCACCCCAGCAGGTAATCC+AGG + chr2.4:9699171-9699190 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 9699147 9700048 9699147 ID=MS.gene31421
chr2.4 mRNA 9699147 9700048 9699147 ID=MS.gene31421.t1;Parent=MS.gene31421
chr2.4 exon 9699856 9700048 9699856 ID=MS.gene31421.t1.exon1;Parent=MS.gene31421.t1
chr2.4 CDS 9699856 9700048 9699856 ID=cds.MS.gene31421.t1;Parent=MS.gene31421.t1
chr2.4 exon 9699147 9699697 9699147 ID=MS.gene31421.t1.exon2;Parent=MS.gene31421.t1
chr2.4 CDS 9699147 9699697 9699147 ID=cds.MS.gene31421.t1;Parent=MS.gene31421.t1
Gene Sequence

>MS.gene31421

GGTGCTGGAATTCATGAGTTACCTGGATTACCTGCTGGGGTGAAGTTTGATCCTACTGATCAAGAGATTCTTGAACATTTGGAGGCAAAAGTGAGGTCTGATATTCAAATGCTTCATCCTTTAATTGATGAGTTCATCCCTACTCTTGAAGGAGAGAATGGAATCTGCTGTACTCATCCAGAGAAGTTACCAGGTCAATTTAAGCCACCAAATATATATATATATATGCCTTATAATTTACTTTCCATTTCTTTTATTCTTTCATAGTTTCTAATAAAAAAAGTCTCATCTTATATCCTATATAATGACAGTTTTTGTGTGTTAATTTAATATTTTACGTCTAATATGCAGGTGTCGGCAAAGATGGGTTGATCCGACATTTCTTTCACAGGCCATCGAAAGCATACACAACAGGAACGAGAAAAAGAAGAAAAGTGCACACAGATGCAGATGGAAGTGAAACAAGATGGCATAAAACAGGTAAAACAAGGCCAGTTTTCGTGAGTGGCAAGTTAAAAGGCTATAAGAAAATCCTTGTGCTTTACACTAACTACAGAAAGCAAAGAAAGCCAGAGAAAACAAATTGGGTTATGCACCAATACCATCTTGGAAATAATGAAGAGGAGAAAGAAGGAGAATTGGTAGTTTCCAAAGTTTTCTACCAAACACAGCCTAGACAATGTGGTGGTTCATTGATGAAAGACTCAGCATCATTTTCTGATAAAAAACTAAATGGTGATCAAGGTGTGAATCATGAGGTGGTTAATCATAAAAACAGTGGATTTGTTGAATACTATAGTACTTCTTTCATATCTTTTGATCAAGGTGAACAACATAGATCAAACAATGCTCAAGTGATTTCTCATTTTCCTGTCCATGATGGTACATCTTTCATTCCTTGA

Protein sequence

>MS.gene31421.t1

GAGIHELPGLPAGVKFDPTDQEILEHLEAKVRSDIQMLHPLIDEFIPTLEGENGICCTHPEKLPGVGKDGLIRHFFHRPSKAYTTGTRKRRKVHTDADGSETRWHKTGKTRPVFVSGKLKGYKKILVLYTNYRKQRKPEKTNWVMHQYHLGNNEEEKEGELVVSKVFYQTQPRQCGGSLMKDSASFSDKKLNGDQGVNHEVVNHKNSGFVEYYSTSFISFDQGEQHRSNNAQVISHFPVHDGTSFIP