AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene05125


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05125.t1 MTR_7g062720 40.541 148 80 3 1 146 1 142 2.06e-31 116
MS.gene05125.t1 MTR_4g037355 40.441 136 61 4 155 290 1 116 1.84e-22 92.0
MS.gene05125.t1 MTR_5g061150 35.354 99 64 0 52 150 38 136 3.64e-18 80.5
MS.gene05125.t1 MTR_4g112730 57.971 69 28 1 143 211 2 69 6.79e-18 78.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 69 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTCTTATTGCCCTCATTAA+TGG 0.201947 3.4:-64781524 MS.gene05125:CDS
AAAGTTGTGGCGTTTATTTC+AGG 0.203868 3.4:+64781208 None:intergenic
TGGATCAGTGGAACAAATTA+AGG 0.229899 3.4:-64781504 MS.gene05125:CDS
GACAAAATTAAGGCAAGATT+TGG 0.257188 3.4:-64781559 MS.gene05125:CDS
TGATTTGGTTACAGCTCTTC+TGG 0.265964 3.4:-64781299 MS.gene05125:CDS
GATTCTTGAGGGAGACGATT+TGG 0.271296 3.4:-64781701 MS.gene05125:CDS
CTCAATTAAGTTGTTGTTCC+AGG 0.274617 3.4:+64781750 None:intergenic
CACGGCCTCCACCACGAAAA+TGG 0.277262 3.4:+64781092 None:intergenic
CATAGGAGAAGATATTAATA+TGG 0.311554 3.4:-64780798 MS.gene05125:CDS
ATTTACTGAATACTCTGATT+TGG 0.313227 3.4:-64781314 MS.gene05125:CDS
CTTGATGATTCTTGTTGGTT+TGG 0.339105 3.4:+64781032 None:intergenic
GAAGAGGTTATATGAATTAT+AGG 0.339571 3.4:-64781069 MS.gene05125:CDS
GGGAGATGTCCCAGAGGAAT+TGG 0.345733 3.4:-64781680 MS.gene05125:CDS
ATTCTTGAGGGAGACGATTT+GGG 0.354692 3.4:-64781700 MS.gene05125:CDS
AAGACAGGTAGTATCATTCT+TGG 0.359896 3.4:+64780835 None:intergenic
TTTACCTTGATGATTCTTGT+TGG 0.362649 3.4:+64781027 None:intergenic
CTTGAGGCTGCTGATTTCAT+AGG 0.363536 3.4:-64780815 MS.gene05125:CDS
GATTTCTAGAGGGACCTTCC+TGG 0.373636 3.4:+64780993 None:intergenic
CATAGCCACCACGTCCTCTT+TGG 0.374275 3.4:+64781158 None:intergenic
CGTGGTGGCTATGCTCGTTT+TGG 0.395091 3.4:-64781148 MS.gene05125:CDS
ACAAAATTAAGGCAAGATTT+GGG 0.416566 3.4:-64781558 MS.gene05125:CDS
TATAACTGAAAAGGAAAAGT+TGG 0.422757 3.4:-64781401 MS.gene05125:CDS
CGTGGTGGAGGCCGTGGAAG+AGG 0.431707 3.4:-64781085 MS.gene05125:CDS
AGATACAAAGAATCTGTTGA+TGG 0.446469 3.4:-64780893 MS.gene05125:CDS
TGGCAAAACTATAACTGAAA+AGG 0.467052 3.4:-64781410 MS.gene05125:CDS
ATCTGTTGATGGAAAGGAAA+AGG 0.477668 3.4:-64780882 MS.gene05125:CDS
AACCACCAGTTGCGTCCTAC+AGG 0.478404 3.4:-64781244 MS.gene05125:CDS
TTGTTCCTGTAGGACGCAAC+TGG 0.480977 3.4:+64781239 None:intergenic
AGACAGGTAGTATCATTCTT+GGG 0.483328 3.4:+64780836 None:intergenic
ATCAAGCACCACATTGATGA+TGG 0.492315 3.4:-64781628 MS.gene05125:CDS
TGCAACAACAATATAGAATG+AGG 0.496917 3.4:-64781339 MS.gene05125:CDS
CTGGAGACAACTACATAACC+TGG 0.502040 3.4:-64781768 MS.gene05125:CDS
AACAAGAATCATCAAGGTAA+AGG 0.504207 3.4:-64781025 MS.gene05125:CDS
TGTTATAGATGCGGAAAGAA+AGG 0.506542 3.4:-64780959 MS.gene05125:CDS
CAAAGAATCTGTTGATGGAA+AGG 0.510693 3.4:-64780888 MS.gene05125:CDS
GATGCGGAAAGAAAGGTCAT+TGG 0.533423 3.4:-64780952 MS.gene05125:CDS
GGACTCGACAAGATTCTTGA+GGG 0.539594 3.4:-64781712 MS.gene05125:CDS
GTTCCACTGATCCATTAATG+AGG 0.543612 3.4:+64781513 None:intergenic
TTGGTTTGGTCATATTTGAG+AGG 0.544297 3.4:+64781046 None:intergenic
TTCCACTGATCCATTAATGA+GGG 0.554709 3.4:+64781514 None:intergenic
CGTGGTCGAGGCAGAAGTCA+TGG 0.554869 3.4:-64781121 MS.gene05125:CDS
GAAATCAGCAGCCTCAAGAC+AGG 0.558951 3.4:+64780820 None:intergenic
TCTTTCCGCATCTATAACAA+CGG 0.562205 3.4:+64780963 None:intergenic
AATTGAGTATCTTGCATGTG+AGG 0.565013 3.4:-64781734 MS.gene05125:CDS
CAAACCAACAAGAATCATCA+AGG 0.574004 3.4:-64781031 MS.gene05125:CDS
AGGTAAAGGTAAAAGCATCC+AGG 0.575090 3.4:-64781011 MS.gene05125:CDS
ATTGAGTATCTTGCATGTGA+GGG 0.575966 3.4:-64781733 MS.gene05125:CDS
GGATATGCCATTGTTCCTGT+AGG 0.578642 3.4:+64781229 None:intergenic
GCAACAACAATATAGAATGA+GGG 0.579939 3.4:-64781338 MS.gene05125:CDS
TGCCCTCATTAATGGATCAG+TGG 0.582546 3.4:-64781516 MS.gene05125:CDS
TTCCTGTAGGACGCAACTGG+TGG 0.593030 3.4:+64781242 None:intergenic
AATTCATATAACCTCTTCCA+CGG 0.595635 3.4:+64781074 None:intergenic
ACGCCCTTGATTAAAAGTTG+TGG 0.608515 3.4:+64781195 None:intergenic
GCAATATCACTTGAGATGCA+TGG 0.608661 3.4:+64781359 None:intergenic
TATAATCGCCAAAGAGGACG+TGG 0.608957 3.4:-64781166 MS.gene05125:CDS
GGGACTCGACAAGATTCTTG+AGG 0.610766 3.4:-64781713 MS.gene05125:CDS
TGTTGATGGAAAGGAAAAGG+AGG 0.612844 3.4:-64780879 MS.gene05125:CDS
TTTGGTTACAGCTCTTCTGG+TGG 0.621241 3.4:-64781296 MS.gene05125:CDS
ACGGTCATCATGATTTCTAG+AGG 0.628064 3.4:+64780982 None:intergenic
GTCATTGGTCTAAAGCATGT+AGG 0.629444 3.4:-64780937 MS.gene05125:CDS
GAATGATACTACCTGTCTTG+AGG 0.637144 3.4:-64780831 MS.gene05125:CDS
GGTGGTTATAATCGCCAAAG+AGG 0.642086 3.4:-64781172 MS.gene05125:CDS
CGGTCATCATGATTTCTAGA+GGG 0.646918 3.4:+64780983 None:intergenic
GATGACCGTTGTTATAGATG+CGG 0.650586 3.4:-64780968 MS.gene05125:CDS
GTTGCGTCCTACAGGAACAA+TGG 0.661047 3.4:-64781236 MS.gene05125:CDS
AAAGGTAAAAGCATCCAGGA+AGG 0.661440 3.4:-64781007 MS.gene05125:CDS
AATCGCCAAAGAGGACGTGG+TGG 0.698905 3.4:-64781163 MS.gene05125:CDS
TTTGTAATCCATCATCAATG+TGG 0.715751 3.4:+64781620 None:intergenic
CGATTTGGGAGATGTCCCAG+AGG 0.725435 3.4:-64781686 MS.gene05125:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATGTTTAAAATTTTGAAT+TGG + chr3.4:64780813-64780832 None:intergenic 10.0%
!!! TTCAAAATTTTAAACATAAC+TGG - chr3.4:64780814-64780833 MS.gene05125:CDS 15.0%
! ACAAAATTAAGGCAAGATTT+GGG - chr3.4:64781043-64781062 MS.gene05125:CDS 25.0%
! ATTTACTGAATACTCTGATT+TGG - chr3.4:64781287-64781306 MS.gene05125:CDS 25.0%
! CATAGGAGAAGATATTAATA+TGG - chr3.4:64781803-64781822 MS.gene05125:CDS 25.0%
! GAAGAGGTTATATGAATTAT+AGG - chr3.4:64781532-64781551 MS.gene05125:CDS 25.0%
! TATAACTGAAAAGGAAAAGT+TGG - chr3.4:64781200-64781219 MS.gene05125:CDS 25.0%
!! AAAAGATCCCAAGATTTTAT+GGG - chr3.4:64781020-64781039 MS.gene05125:CDS 25.0%
!! AATTTTGTCCCATAAAATCT+TGG + chr3.4:64781031-64781050 None:intergenic 25.0%
!! ATTGCACAATTAGAATTTTG+TGG - chr3.4:64781171-64781190 MS.gene05125:CDS 25.0%
!! ATTTTGTCCCATAAAATCTT+GGG + chr3.4:64781030-64781049 None:intergenic 25.0%
!! GATTTTATGGGACAAAATTA+AGG - chr3.4:64781032-64781051 MS.gene05125:CDS 25.0%
!!! CAACAACAGTTTTATAATCT+TGG + chr3.4:64781125-64781144 None:intergenic 25.0%
AACAAGAATCATCAAGGTAA+AGG - chr3.4:64781576-64781595 MS.gene05125:CDS 30.0%
AATTCATATAACCTCTTCCA+CGG + chr3.4:64781530-64781549 None:intergenic 30.0%
AGATACAAAGAATCTGTTGA+TGG - chr3.4:64781708-64781727 MS.gene05125:CDS 30.0%
GACAAAATTAAGGCAAGATT+TGG - chr3.4:64781042-64781061 MS.gene05125:CDS 30.0%
GCAACAACAATATAGAATGA+GGG - chr3.4:64781263-64781282 MS.gene05125:CDS 30.0%
TGCAACAACAATATAGAATG+AGG - chr3.4:64781262-64781281 MS.gene05125:CDS 30.0%
TGGCAAAACTATAACTGAAA+AGG - chr3.4:64781191-64781210 MS.gene05125:CDS 30.0%
TTTGTAATCCATCATCAATG+TGG + chr3.4:64780984-64781003 None:intergenic 30.0%
! CAAAAGATCCCAAGATTTTA+TGG - chr3.4:64781019-64781038 MS.gene05125:CDS 30.0%
! TATCTTTTACACAGATACTC+TGG + chr3.4:64781693-64781712 None:intergenic 30.0%
! TTTACCTTGATGATTCTTGT+TGG + chr3.4:64781577-64781596 None:intergenic 30.0%
AAAGTTGTGGCGTTTATTTC+AGG + chr3.4:64781396-64781415 None:intergenic 35.0%
AAGACAGGTAGTATCATTCT+TGG + chr3.4:64781769-64781788 None:intergenic 35.0%
AATTGAGTATCTTGCATGTG+AGG - chr3.4:64780867-64780886 MS.gene05125:CDS 35.0%
AGACAGGTAGTATCATTCTT+GGG + chr3.4:64781768-64781787 None:intergenic 35.0%
ATTGAGTATCTTGCATGTGA+GGG - chr3.4:64780868-64780887 MS.gene05125:CDS 35.0%
CAAACCAACAAGAATCATCA+AGG - chr3.4:64781570-64781589 MS.gene05125:CDS 35.0%
TCTTTCCGCATCTATAACAA+CGG + chr3.4:64781641-64781660 None:intergenic 35.0%
TGGATCAGTGGAACAAATTA+AGG - chr3.4:64781097-64781116 MS.gene05125:CDS 35.0%
TGTTATAGATGCGGAAAGAA+AGG - chr3.4:64781642-64781661 MS.gene05125:CDS 35.0%
TTCCACTGATCCATTAATGA+GGG + chr3.4:64781090-64781109 None:intergenic 35.0%
! AACGCCACAACTTTTAATCA+AGG - chr3.4:64781402-64781421 MS.gene05125:CDS 35.0%
! ACGCCACAACTTTTAATCAA+GGG - chr3.4:64781403-64781422 MS.gene05125:CDS 35.0%
! ATCTGTTGATGGAAAGGAAA+AGG - chr3.4:64781719-64781738 MS.gene05125:CDS 35.0%
! CAAAGAATCTGTTGATGGAA+AGG - chr3.4:64781713-64781732 MS.gene05125:CDS 35.0%
! CTCAATTAAGTTGTTGTTCC+AGG + chr3.4:64780854-64780873 None:intergenic 35.0%
! CTTGATGATTCTTGTTGGTT+TGG + chr3.4:64781572-64781591 None:intergenic 35.0%
! TTTTCTTTGCCAATTCCTCT+GGG + chr3.4:64780933-64780952 None:intergenic 35.0%
! TTTTTCTTTGCCAATTCCTC+TGG + chr3.4:64780934-64780953 None:intergenic 35.0%
!! AGTCTTATTGCCCTCATTAA+TGG - chr3.4:64781077-64781096 MS.gene05125:CDS 35.0%
!! TTGGTTTGGTCATATTTGAG+AGG + chr3.4:64781558-64781577 None:intergenic 35.0%
ACGCCCTTGATTAAAAGTTG+TGG + chr3.4:64781409-64781428 None:intergenic 40.0%
ATCAAGCACCACATTGATGA+TGG - chr3.4:64780973-64780992 MS.gene05125:CDS 40.0%
ATTCTTGAGGGAGACGATTT+GGG - chr3.4:64780901-64780920 MS.gene05125:CDS 40.0%
GAATGATACTACCTGTCTTG+AGG - chr3.4:64781770-64781789 MS.gene05125:CDS 40.0%
GATGACCGTTGTTATAGATG+CGG - chr3.4:64781633-64781652 MS.gene05125:CDS 40.0%
GCAATATCACTTGAGATGCA+TGG + chr3.4:64781245-64781264 None:intergenic 40.0%
GTTCCACTGATCCATTAATG+AGG + chr3.4:64781091-64781110 None:intergenic 40.0%
! ACAACTTTTAATCAAGGGCG+TGG - chr3.4:64781408-64781427 MS.gene05125:CDS 40.0%
! ACGGTCATCATGATTTCTAG+AGG + chr3.4:64781622-64781641 None:intergenic 40.0%
! CGGTCATCATGATTTCTAGA+GGG + chr3.4:64781621-64781640 None:intergenic 40.0%
! GTCATTGGTCTAAAGCATGT+AGG - chr3.4:64781664-64781683 MS.gene05125:CDS 40.0%
! TGTTGATGGAAAGGAAAAGG+AGG - chr3.4:64781722-64781741 MS.gene05125:CDS 40.0%
!! AAAGGTAAAAGCATCCAGGA+AGG - chr3.4:64781594-64781613 MS.gene05125:CDS 40.0%
!! AGGTAAAGGTAAAAGCATCC+AGG - chr3.4:64781590-64781609 MS.gene05125:CDS 40.0%
!! TGATTTGGTTACAGCTCTTC+TGG - chr3.4:64781302-64781321 MS.gene05125:CDS 40.0%
CTGGAGACAACTACATAACC+TGG - chr3.4:64780833-64780852 MS.gene05125:CDS 45.0%
GATGCGGAAAGAAAGGTCAT+TGG - chr3.4:64781649-64781668 MS.gene05125:CDS 45.0%
GATTCTTGAGGGAGACGATT+TGG - chr3.4:64780900-64780919 MS.gene05125:CDS 45.0%
GGACTCGACAAGATTCTTGA+GGG - chr3.4:64780889-64780908 MS.gene05125:CDS 45.0%
GGATATGCCATTGTTCCTGT+AGG + chr3.4:64781375-64781394 None:intergenic 45.0%
GGTGGTTATAATCGCCAAAG+AGG - chr3.4:64781429-64781448 MS.gene05125:CDS 45.0%
TATAATCGCCAAAGAGGACG+TGG - chr3.4:64781435-64781454 MS.gene05125:CDS 45.0%
TTTGGTTACAGCTCTTCTGG+TGG - chr3.4:64781305-64781324 MS.gene05125:CDS 45.0%
! CATGGTCGAAACCATTTTCG+TGG - chr3.4:64781498-64781517 MS.gene05125:CDS 45.0%
! TGCCCTCATTAATGGATCAG+TGG - chr3.4:64781085-64781104 MS.gene05125:CDS 45.0%
!! ACTTTTAATCAAGGGCGTGG+TGG - chr3.4:64781411-64781430 MS.gene05125:CDS 45.0%
!! CTTGAGGCTGCTGATTTCAT+AGG - chr3.4:64781786-64781805 MS.gene05125:CDS 45.0%
GAAATCAGCAGCCTCAAGAC+AGG + chr3.4:64781784-64781803 None:intergenic 50.0%
GGGACTCGACAAGATTCTTG+AGG - chr3.4:64780888-64780907 MS.gene05125:CDS 50.0%
GTTGCGTCCTACAGGAACAA+TGG - chr3.4:64781365-64781384 MS.gene05125:CDS 50.0%
TTGTTCCTGTAGGACGCAAC+TGG + chr3.4:64781365-64781384 None:intergenic 50.0%
! CGAAACCATTTTCGTGGTGG+AGG - chr3.4:64781504-64781523 MS.gene05125:CDS 50.0%
! GATTTCTAGAGGGACCTTCC+TGG + chr3.4:64781611-64781630 None:intergenic 50.0%
! GGTCGAAACCATTTTCGTGG+TGG - chr3.4:64781501-64781520 MS.gene05125:CDS 50.0%
!! TATGCTCGTTTTGGTGGTCG+TGG - chr3.4:64781462-64781481 MS.gene05125:CDS 50.0%
AACCACCAGTTGCGTCCTAC+AGG - chr3.4:64781357-64781376 MS.gene05125:CDS 55.0%
AATCGCCAAAGAGGACGTGG+TGG - chr3.4:64781438-64781457 MS.gene05125:CDS 55.0%
CATAGCCACCACGTCCTCTT+TGG + chr3.4:64781446-64781465 None:intergenic 55.0%
GGGAGATGTCCCAGAGGAAT+TGG - chr3.4:64780921-64780940 MS.gene05125:CDS 55.0%
TTCCTGTAGGACGCAACTGG+TGG + chr3.4:64781362-64781381 None:intergenic 55.0%
! CGATTTGGGAGATGTCCCAG+AGG - chr3.4:64780915-64780934 MS.gene05125:CDS 55.0%
! CGTGGTGGCTATGCTCGTTT+TGG - chr3.4:64781453-64781472 MS.gene05125:CDS 55.0%
! GGTGGCTATGCTCGTTTTGG+TGG - chr3.4:64781456-64781475 MS.gene05125:CDS 55.0%
CACGGCCTCCACCACGAAAA+TGG + chr3.4:64781512-64781531 None:intergenic 60.0%
CGTGGTCGAGGCAGAAGTCA+TGG - chr3.4:64781480-64781499 MS.gene05125:CDS 60.0%
!! CATTTTCGTGGTGGAGGCCG+TGG - chr3.4:64781510-64781529 MS.gene05125:CDS 60.0%
!! CGTTTTGGTGGTCGTGGTCG+AGG - chr3.4:64781468-64781487 MS.gene05125:CDS 60.0%
! CGTGGTGGAGGCCGTGGAAG+AGG - chr3.4:64781516-64781535 MS.gene05125:CDS 70.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 64780793 64781830 64780793 ID=MS.gene05125
chr3.4 mRNA 64780793 64781830 64780793 ID=MS.gene05125.t1;Parent=MS.gene05125
chr3.4 exon 64780793 64781830 64780793 ID=MS.gene05125.t1.exon1;Parent=MS.gene05125.t1
chr3.4 CDS 64780793 64781830 64780793 ID=cds.MS.gene05125.t1;Parent=MS.gene05125.t1
Gene Sequence

>MS.gene05125

ATGTCAAATGTTAAGCACCAATTCAAAATTTTAAACATAACTGGAGACAACTACATAACCTGGAACAACAACTTAATTGAGTATCTTGCATGTGAGGGACTCGACAAGATTCTTGAGGGAGACGATTTGGGAGATGTCCCAGAGGAATTGGCAAAGAAAAAATCAAAAGTAAATCGTATAATCAAGCACCACATTGATGATGGATTACAAACAGAGTATTCAAATGCAAAAGATCCCAAGATTTTATGGGACAAAATTAAGGCAAGATTTGGGCATCAGAAGAAAGTCTTATTGCCCTCATTAATGGATCAGTGGAACAAATTAAGGTTCCAAGATTATAAAACTGTTGTTGCATATAACTCTGCTATGCATCAAATTATTGCACAATTAGAATTTTGTGGCAAAACTATAACTGAAAAGGAAAAGTTGGAGAAAACATTCTCAACTTTCCATGCATCTCAAGTGATATTGCAACAACAATATAGAATGAGGGAATTTACTGAATACTCTGATTTGGTTACAGCTCTTCTGGTGGCTGAACAAAATAATGAGCTGCTCATTAAAAACCACCAGTTGCGTCCTACAGGAACAATGGCATATCCTGAAATAAACGCCACAACTTTTAATCAAGGGCGTGGTGGTTATAATCGCCAAAGAGGACGTGGTGGCTATGCTCGTTTTGGTGGTCGTGGTCGAGGCAGAAGTCATGGTCGAAACCATTTTCGTGGTGGAGGCCGTGGAAGAGGTTATATGAATTATAGGCCTCTCAAATATGACCAAACCAACAAGAATCATCAAGGTAAAGGTAAAAGCATCCAGGAAGGTCCCTCTAGAAATCATGATGACCGTTGTTATAGATGCGGAAAGAAAGGTCATTGGTCTAAAGCATGTAGGACACCAGAGTATCTGTGTAAAAGATACAAAGAATCTGTTGATGGAAAGGAAAAGGAGGTTAATTTTAATGAAATTGAACCCAAGAATGATACTACCTGTCTTGAGGCTGCTGATTTCATAGGAGAAGATATTAATATGGATTAA

Protein sequence

>MS.gene05125.t1

MSNVKHQFKILNITGDNYITWNNNLIEYLACEGLDKILEGDDLGDVPEELAKKKSKVNRIIKHHIDDGLQTEYSNAKDPKILWDKIKARFGHQKKVLLPSLMDQWNKLRFQDYKTVVAYNSAMHQIIAQLEFCGKTITEKEKLEKTFSTFHASQVILQQQYRMREFTEYSDLVTALLVAEQNNELLIKNHQLRPTGTMAYPEINATTFNQGRGGYNRQRGRGGYARFGGRGRGRSHGRNHFRGGGRGRGYMNYRPLKYDQTNKNHQGKGKSIQEGPSRNHDDRCYRCGKKGHWSKACRTPEYLCKRYKESVDGKEKEVNFNEIEPKNDTTCLEAADFIGEDINMD