AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32061

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32061.t1 MTR_1g072420 96.296 432 16 0 3 434 4 435 0.0 852
MS.gene32061.t1 MTR_1g072450 87.963 432 49 3 4 434 5 434 0.0 765
MS.gene32061.t1 MTR_1g072380 78.986 414 77 4 29 434 40 451 0.0 648
MS.gene32061.t1 MTR_2g022450 54.545 429 185 6 10 433 6 429 1.86e-161 462
MS.gene32061.t1 MTR_1g093100 52.539 453 179 8 1 434 1 436 2.11e-156 449
MS.gene32061.t1 MTR_4g117090 46.226 424 200 10 28 428 33 451 4.53e-114 342
MS.gene32061.t1 MTR_7g113790 40.716 447 225 14 7 432 8 435 3.10e-99 303
MS.gene32061.t1 MTR_2g022430 42.989 435 204 13 9 426 13 420 5.43e-96 295
MS.gene32061.t1 MTR_2g022370 42.466 438 219 11 2 429 5 419 3.33e-92 285
MS.gene32061.t1 MTR_2g022380 42.438 443 221 10 1 429 1 423 5.07e-92 285
MS.gene32061.t1 MTR_2g022460 41.505 412 205 10 31 432 26 411 1.86e-91 283
MS.gene32061.t1 MTR_2g022350 42.081 442 218 11 1 426 1 420 5.60e-90 280
MS.gene32061.t1 MTR_2g022320 42.067 416 199 10 32 427 24 417 1.56e-87 273
MS.gene32061.t1 MTR_2g022490 41.147 401 191 10 28 406 27 404 7.23e-85 266
MS.gene32061.t1 MTR_2g022480 39.192 421 224 11 3 406 5 410 6.93e-83 261
MS.gene32061.t1 MTR_0252s0030 43.779 217 101 5 190 395 140 346 2.18e-44 158
MS.gene32061.t1 MTR_1g093130 42.771 166 75 5 230 394 6 152 1.02e-28 111
MS.gene32061.t1 MTR_7g062360 22.762 391 240 15 54 417 68 423 4.18e-19 89.4
MS.gene32061.t1 MTR_7g105850 25.485 412 222 20 46 414 91 460 1.18e-17 85.1
MS.gene32061.t1 MTR_3g046300 24.679 389 230 15 54 417 106 456 1.14e-16 82.4
MS.gene32061.t1 MTR_5g091130 23.196 388 238 16 54 417 200 551 7.09e-15 77.0
MS.gene32061.t1 MTR_3g068000 22.821 390 229 17 59 415 87 437 1.10e-14 76.3
MS.gene32061.t1 MTR_2g022330 61.538 52 20 0 377 428 34 85 1.05e-13 67.0
MS.gene32061.t1 MTR_8g103880 24.872 390 214 19 43 410 114 446 3.88e-13 71.2
MS.gene32061.t1 MTR_3g028120 23.822 382 222 17 54 414 98 431 6.08e-13 70.9
MS.gene32061.t1 MTR_1g111350 25.243 412 229 18 25 415 110 463 9.75e-13 70.1
MS.gene32061.t1 MTR_2g055840 23.438 384 230 15 54 412 22 366 3.18e-11 65.1
MS.gene32061.t1 MTR_5g016260 22.807 399 217 17 46 414 89 426 3.40e-11 65.5
MS.gene32061.t1 MTR_5g016260 22.760 413 222 18 46 428 89 434 5.36e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32061.t1 AT1G03220 64.252 428 147 4 10 434 9 433 0.0 550
MS.gene32061.t1 AT1G03230 62.169 415 151 4 23 434 23 434 0.0 516
MS.gene32061.t1 AT5G19120 36.077 413 208 10 12 414 9 375 2.10e-60 201
MS.gene32061.t1 AT5G19110 30.986 426 259 8 9 428 5 401 2.61e-55 189
MS.gene32061.t1 AT5G19110 30.986 426 259 8 9 428 41 437 6.99e-55 188
MS.gene32061.t1 AT5G48430 31.954 435 251 16 6 429 3 403 2.08e-50 176
MS.gene32061.t1 AT5G19100 34.840 376 185 14 62 426 58 384 1.16e-45 163
MS.gene32061.t1 AT2G39710 26.087 391 213 19 59 415 78 426 4.66e-21 95.5
MS.gene32061.t1 AT3G25700 25.476 420 228 19 35 415 72 445 1.11e-18 88.2
MS.gene32061.t1 AT5G02190 21.228 391 236 15 59 415 86 438 1.99e-13 72.4
MS.gene32061.t1 AT5G36260 22.897 428 258 18 18 415 44 429 1.96e-12 69.3
MS.gene32061.t1 AT3G52500 21.324 408 249 16 46 415 90 463 1.43e-11 66.6
MS.gene32061.t1 AT3G54400 24.041 391 218 17 46 415 88 420 3.49e-11 65.1
MS.gene32061.t1 AT3G02740 22.762 391 241 14 46 415 10 360 7.24e-11 64.3

Find 110 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTCTCTCAACAATCTTTC+AGG 0.152886 1.2:-55310890 MS.gene32061:CDS
TGATATGGCTGCTTCTAAAT+TGG 0.163227 1.2:-55309737 MS.gene32061:CDS
CACAATCAACCCATAGAAAT+TGG 0.188301 1.2:+55310751 None:intergenic
TTGAGATGGAAGAGCAATTT+TGG 0.214100 1.2:+55310401 None:intergenic
GCACCAACTTTCTTACTAAA+AGG 0.223724 1.2:-55310465 MS.gene32061:CDS
ATCTCGGTGGCCAATTTCTA+TGG 0.242839 1.2:-55310761 MS.gene32061:CDS
GTGGGACTAGTGGGGTTCTT+TGG 0.257108 1.2:+55310799 None:intergenic
TGTAATGAAGTGTTGAAAAT+TGG 0.292358 1.2:+55310944 None:intergenic
GGATTTAGCTCCTTGCTCTT+TGG 0.298601 1.2:-55309715 MS.gene32061:CDS
TTATTCGGGGATGGACCTTA+TGG 0.328054 1.2:-55310312 MS.gene32061:CDS
TTATCAATCCAATCCTCTAA+TGG 0.328408 1.2:-55310537 MS.gene32061:CDS
GCTTCTGGCATGGCTGGTCT+TGG 0.335616 1.2:-55310429 MS.gene32061:CDS
AATGTGGTTTGGAGGATATT+TGG 0.337063 1.2:-55309883 MS.gene32061:CDS
GGACTTGCAAGTGGTGCTTC+TGG 0.358517 1.2:-55310444 MS.gene32061:CDS
TAATCTTGTGCAATTTGATA+TGG 0.358657 1.2:-55309752 MS.gene32061:CDS
GGTTGATTTGAGCTTTGTAT+TGG 0.360990 1.2:+55310820 None:intergenic
ACTGGGAGAACTAGGGCTTT+TGG 0.362155 1.2:+55310867 None:intergenic
GATATGGCTGCTTCTAAATT+GGG 0.365374 1.2:-55309736 MS.gene32061:CDS
TCCTGGGTTGAAGCCATTAG+AGG 0.369624 1.2:+55310524 None:intergenic
ATCACCACAGCCGTCGGAAT+TGG 0.371692 1.2:+55310665 None:intergenic
TGCAAAATGAAAATGTGGTT+TGG 0.372624 1.2:-55309894 MS.gene32061:CDS
AGTGGTGCTTCTGGCATGGC+TGG 0.372982 1.2:-55310435 MS.gene32061:CDS
TTGTGTCTTGGATTTGTGAA+TGG 0.380483 1.2:-55309826 MS.gene32061:CDS
AAAGCAGAAGCTGTGCTAAC+AGG 0.404309 1.2:+55310228 None:intergenic
CGGAATTGGCGAGGGAGCAT+TGG 0.411697 1.2:+55310679 None:intergenic
CAGGTGTTGTTGTTGCAGCC+AGG 0.418057 1.2:+55310624 None:intergenic
ACAAGGACTTCAATTGTGAT+TGG 0.421714 1.2:-55309793 MS.gene32061:CDS
AAACATAAAGAGGGTGGATT+CGG 0.425811 1.2:-55309995 MS.gene32061:CDS
TCTTCCATCTCAACTAGCTT+CGG 0.431750 1.2:-55310391 MS.gene32061:CDS
GGAATTGGCGAGGGAGCATT+GGG 0.432376 1.2:+55310680 None:intergenic
TATTTGTCTTTCCTCTTCAA+AGG 0.433460 1.2:-55310343 MS.gene32061:CDS
GCTGGTGGCGGTGTGGGTGA+TGG 0.441452 1.2:+55310581 None:intergenic
TGGGCTGAGCGACACCGTGC+AGG 0.443405 1.2:+55310699 None:intergenic
TGTGGGTGATGGAATTGTCC+GGG 0.446715 1.2:+55310592 None:intergenic
GATATTTGGTGCAAATTCAA+TGG 0.448768 1.2:-55309869 MS.gene32061:CDS
TTGTTGTTGCAGCCAGGCTT+TGG 0.449409 1.2:+55310630 None:intergenic
GGTTGAAGCCATTAGAGGAT+TGG 0.451183 1.2:+55310529 None:intergenic
GTGTGGGTGATGGAATTGTC+CGG 0.454258 1.2:+55310591 None:intergenic
CTCGCCAATTCCGACGGCTG+TGG 0.458653 1.2:-55310669 MS.gene32061:CDS
GAAGCTTCTAAAACGGTGTA+AGG 0.459508 1.2:+55310066 None:intergenic
GAAACTGCGACATTTCGTCC+TGG 0.459544 1.2:+55310507 None:intergenic
GTCTGAATCAAGAACAATGT+TGG 0.467879 1.2:+55310281 None:intergenic
AGCAAGTTCACCGCTGGTGG+CGG 0.469786 1.2:+55310569 None:intergenic
ATTTGTCTTTCCTCTTCAAA+GGG 0.478971 1.2:-55310342 MS.gene32061:CDS
TCTGAATCAAGAACAATGTT+GGG 0.479866 1.2:+55310282 None:intergenic
TTCACCGCTGGTGGCGGTGT+GGG 0.483515 1.2:+55310575 None:intergenic
GTAAATAGAAGCTTCTAAAA+CGG 0.490344 1.2:+55310059 None:intergenic
TGTTTCTCATCACCAAAGCC+TGG 0.494972 1.2:-55310642 MS.gene32061:CDS
TCCTCTAATGGCTTCAACCC+AGG 0.495018 1.2:-55310525 MS.gene32061:CDS
AAACTGCGACATTTCGTCCT+GGG 0.498880 1.2:+55310508 None:intergenic
AAGTTGGTGCACATGAGAAT+AGG 0.499306 1.2:+55310478 None:intergenic
ATAGACAACAACGGCGTTGG+TGG 0.502656 1.2:-55310111 MS.gene32061:CDS
TGCAAGTGGTGCTTCTGGCA+TGG 0.502670 1.2:-55310439 MS.gene32061:CDS
TCTATAGACAACAACGGCGT+TGG 0.509782 1.2:-55310114 MS.gene32061:CDS
TGTCTTGGATTTGTGAATGG+TGG 0.517202 1.2:-55309823 MS.gene32061:CDS
CTGAACGTCATCGTCGATCT+CGG 0.517558 1.2:-55310777 MS.gene32061:CDS
ACACCGTGCAGGGCGGTATG+TGG 0.520199 1.2:+55310710 None:intergenic
GATTGGAGGGTATCAGTTGG+AGG 0.520806 1.2:-55309776 MS.gene32061:CDS
TTAGTGTAACAAAATTCAAA+AGG 0.525717 1.2:+55309967 None:intergenic
TCTCGGTGGCCAATTTCTAT+GGG 0.528725 1.2:-55310760 MS.gene32061:CDS
GAAAGATTGTTGAGAGAAAG+TGG 0.530349 1.2:+55310893 None:intergenic
AATGTGACTGGGACACGATT+GGG 0.533667 1.2:-55309946 MS.gene32061:CDS
TAATGTGACTGGGACACGAT+TGG 0.534463 1.2:-55309947 MS.gene32061:CDS
GTTCTCCCAGTCACAAAAGA+TGG 0.534725 1.2:-55310855 MS.gene32061:CDS
TGACGTTCAGTGGGACTAGT+GGG 0.536843 1.2:+55310790 None:intergenic
ATTGTCCGGGGTGACACCAC+AGG 0.542125 1.2:+55310605 None:intergenic
AGGACTTCAATTGTGATTGG+AGG 0.545988 1.2:-55309790 MS.gene32061:CDS
GGGCTGAGCGACACCGTGCA+GGG 0.548753 1.2:+55310700 None:intergenic
ATCTTCAGCAAGTTCACCGC+TGG 0.549080 1.2:+55310563 None:intergenic
AAAATGAAAATGTGGTTTGG+AGG 0.560548 1.2:-55309891 MS.gene32061:CDS
TTCAGCAAGTTCACCGCTGG+TGG 0.561694 1.2:+55310566 None:intergenic
GTTCACCGCTGGTGGCGGTG+TGG 0.562370 1.2:+55310574 None:intergenic
GGACTTCAATTGTGATTGGA+GGG 0.563151 1.2:-55309789 MS.gene32061:CDS
AAAGCCGAAGCTAGTTGAGA+TGG 0.563385 1.2:+55310387 None:intergenic
TGAATGGTGGTAAGAATACA+AGG 0.567635 1.2:-55309810 MS.gene32061:CDS
AATGGTGAATATAAATGATG+AGG 0.569327 1.2:-55309851 MS.gene32061:CDS
ACAGCTTCTGCTTTCTCACA+AGG 0.569954 1.2:-55310219 MS.gene32061:CDS
AGATCGACGATGACGTTCAG+TGG 0.573829 1.2:+55310780 None:intergenic
TAAAACAACTCCCTTTGAAG+AGG 0.576429 1.2:+55310332 None:intergenic
CTGCTGCAAGAAACATAAAG+AGG 0.579468 1.2:-55310005 MS.gene32061:CDS
TGCTCCCTCGCCAATTCCGA+CGG 0.580900 1.2:-55310675 MS.gene32061:CDS
TGTGATTGGAGGGTATCAGT+TGG 0.585357 1.2:-55309779 MS.gene32061:CDS
TTACTAAAAGGACTTGCAAG+TGG 0.586199 1.2:-55310453 MS.gene32061:CDS
TGGTGGAACAAAGATTAGTA+CGG 0.586226 1.2:-55310094 MS.gene32061:CDS
CACAGCCGTCGGAATTGGCG+AGG 0.587987 1.2:+55310670 None:intergenic
CCTCCACATACCGCCCTGCA+CGG 0.588163 1.2:-55310713 MS.gene32061:CDS
TTTGTTACACTAATGTGACT+GGG 0.589924 1.2:-55309957 MS.gene32061:CDS
CCTTCAGCCGAGTATTTCAT+CGG 0.590645 1.2:-55310192 MS.gene32061:CDS
ATGTTGGGAAGAAAACCATA+AGG 0.595062 1.2:+55310297 None:intergenic
GTGTTGAAAATTGGAAGCCA+TGG 0.599453 1.2:+55310953 None:intergenic
TTTCACGCCGATGAAATACT+CGG 0.606909 1.2:+55310185 None:intergenic
ACAGCCGTCGGAATTGGCGA+GGG 0.612804 1.2:+55310671 None:intergenic
CAACACCTGTGGTGTCACCC+CGG 0.614141 1.2:-55310610 MS.gene32061:CDS
ATGACGTTCAGTGGGACTAG+TGG 0.614911 1.2:+55310789 None:intergenic
CCGATGAAATACTCGGCTGA+AGG 0.617301 1.2:+55310192 None:intergenic
CCGTGCAGGGCGGTATGTGG+AGG 0.617989 1.2:+55310713 None:intergenic
TGCTGCAAGAAACATAAAGA+GGG 0.632185 1.2:-55310004 MS.gene32061:CDS
AGTTTGTCGTCCAAAGAGCA+AGG 0.633294 1.2:+55309705 None:intergenic
ATCACCCACACCGCCACCAG+CGG 0.641652 1.2:-55310579 MS.gene32061:CDS
GATCGACGATGACGTTCAGT+GGG 0.650404 1.2:+55310781 None:intergenic
GACGTTCAGTGGGACTAGTG+GGG 0.659322 1.2:+55310791 None:intergenic
AAAAGGCATCTAGGCAGTGG+AGG 0.665153 1.2:+55309660 None:intergenic
CTAACAGGATTGATCAAAAG+CGG 0.665242 1.2:+55310243 None:intergenic
TGCAAGAAACATAAAGAGGG+TGG 0.665566 1.2:-55310001 MS.gene32061:CDS
GAAACAATCACCACAGCCGT+CGG 0.679467 1.2:+55310659 None:intergenic
GTGGGTGATGGAATTGTCCG+GGG 0.682973 1.2:+55310593 None:intergenic
GGCTGCAACAACAACACCTG+TGG 0.687479 1.2:-55310621 MS.gene32061:CDS
AACGTCATCGTCGATCTCGG+TGG 0.719707 1.2:-55310774 MS.gene32061:CDS
CTGAGCGACACCGTGCAGGG+CGG 0.729304 1.2:+55310703 None:intergenic
AAAGATTGTTGAGAGAAAGT+GGG 0.736615 1.2:+55310894 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAGTGTAACAAAATTCAAA+AGG + chr1.2:55310656-55310675 None:intergenic 20.0%
! AAATGAAGAAAAATAGGAGT+AGG + chr1.2:55309704-55309723 None:intergenic 25.0%
! AATGAAGAAAAATAGGAGTA+GGG + chr1.2:55309703-55309722 None:intergenic 25.0%
! AATGGTGAATATAAATGATG+AGG - chr1.2:55310769-55310788 MS.gene32061:CDS 25.0%
! TAATCTTGTGCAATTTGATA+TGG - chr1.2:55310868-55310887 MS.gene32061:CDS 25.0%
!! CTTTTTGCAAAATGAAAATG+TGG - chr1.2:55310721-55310740 MS.gene32061:CDS 25.0%
!! GTAAATAGAAGCTTCTAAAA+CGG + chr1.2:55310564-55310583 None:intergenic 25.0%
!! TTGTTTTCTATAGACAACAA+CGG - chr1.2:55310500-55310519 MS.gene32061:CDS 25.0%
!!! TGTAATGAAGTGTTGAAAAT+TGG + chr1.2:55309679-55309698 None:intergenic 25.0%
AAAGATTGTTGAGAGAAAGT+GGG + chr1.2:55309729-55309748 None:intergenic 30.0%
ATTTGTCTTTCCTCTTCAAA+GGG - chr1.2:55310278-55310297 MS.gene32061:CDS 30.0%
TATTTGTCTTTCCTCTTCAA+AGG - chr1.2:55310277-55310296 MS.gene32061:CDS 30.0%
TCTGAATCAAGAACAATGTT+GGG + chr1.2:55310341-55310360 None:intergenic 30.0%
TGCAAAATGAAAATGTGGTT+TGG - chr1.2:55310726-55310745 MS.gene32061:CDS 30.0%
TGGGTGAAATGAAGAAAAAT+AGG + chr1.2:55309710-55309729 None:intergenic 30.0%
TTATCAATCCAATCCTCTAA+TGG - chr1.2:55310083-55310102 MS.gene32061:CDS 30.0%
TTTGTTACACTAATGTGACT+GGG - chr1.2:55310663-55310682 MS.gene32061:CDS 30.0%
! AAAATGAAAATGTGGTTTGG+AGG - chr1.2:55310729-55310748 MS.gene32061:CDS 30.0%
! AGTCCTTTTAGTAAGAAAGT+TGG + chr1.2:55310161-55310180 None:intergenic 30.0%
! TTTTGTTACACTAATGTGAC+TGG - chr1.2:55310662-55310681 MS.gene32061:CDS 30.0%
!! GATATTTGGTGCAAATTCAA+TGG - chr1.2:55310751-55310770 MS.gene32061:CDS 30.0%
!! TCAAAGGGAGTTGTTTTATT+CGG - chr1.2:55310293-55310312 MS.gene32061:CDS 30.0%
ACAAGGACTTCAATTGTGAT+TGG - chr1.2:55310827-55310846 MS.gene32061:CDS 35.0%
ATGTTGGGAAGAAAACCATA+AGG + chr1.2:55310326-55310345 None:intergenic 35.0%
CACAATCAACCCATAGAAAT+TGG + chr1.2:55309872-55309891 None:intergenic 35.0%
CTAACAGGATTGATCAAAAG+CGG + chr1.2:55310380-55310399 None:intergenic 35.0%
CTTTCTCTCAACAATCTTTC+AGG - chr1.2:55309730-55309749 MS.gene32061:CDS 35.0%
GAAAGATTGTTGAGAGAAAG+TGG + chr1.2:55309730-55309749 None:intergenic 35.0%
GATATGGCTGCTTCTAAATT+GGG - chr1.2:55310884-55310903 MS.gene32061:CDS 35.0%
GCACCAACTTTCTTACTAAA+AGG - chr1.2:55310155-55310174 MS.gene32061:CDS 35.0%
GTCTGAATCAAGAACAATGT+TGG + chr1.2:55310342-55310361 None:intergenic 35.0%
TGAATGGTGGTAAGAATACA+AGG - chr1.2:55310810-55310829 MS.gene32061:CDS 35.0%
TGATATGGCTGCTTCTAAAT+TGG - chr1.2:55310883-55310902 MS.gene32061:CDS 35.0%
TGCAAAAAGAGCTCAATAGT+AGG + chr1.2:55310710-55310729 None:intergenic 35.0%
TGCTGCAAGAAACATAAAGA+GGG - chr1.2:55310616-55310635 MS.gene32061:CDS 35.0%
TGGTGGAACAAAGATTAGTA+CGG - chr1.2:55310526-55310545 MS.gene32061:CDS 35.0%
TTACTAAAAGGACTTGCAAG+TGG - chr1.2:55310167-55310186 MS.gene32061:CDS 35.0%
TTGTGTCTTGGATTTGTGAA+TGG - chr1.2:55310794-55310813 MS.gene32061:CDS 35.0%
! AAACATAAAGAGGGTGGATT+CGG - chr1.2:55310625-55310644 MS.gene32061:CDS 35.0%
! AATGTGGTTTGGAGGATATT+TGG - chr1.2:55310737-55310756 MS.gene32061:CDS 35.0%
! TAAAACAACTCCCTTTGAAG+AGG + chr1.2:55310291-55310310 None:intergenic 35.0%
! TTGAGATGGAAGAGCAATTT+TGG + chr1.2:55310222-55310241 None:intergenic 35.0%
!! AAAGGGAGTTGTTTTATTCG+GGG - chr1.2:55310295-55310314 MS.gene32061:CDS 35.0%
!! AATGATGAGGTTTTGTGTCT+TGG - chr1.2:55310782-55310801 MS.gene32061:CDS 35.0%
!! CAAAGGGAGTTGTTTTATTC+GGG - chr1.2:55310294-55310313 MS.gene32061:CDS 35.0%
!! GGTTGATTTGAGCTTTGTAT+TGG + chr1.2:55309803-55309822 None:intergenic 35.0%
AGGACTTCAATTGTGATTGG+AGG - chr1.2:55310830-55310849 MS.gene32061:CDS 40.0%
CTGCTGCAAGAAACATAAAG+AGG - chr1.2:55310615-55310634 MS.gene32061:CDS 40.0%
GGACTTCAATTGTGATTGGA+GGG - chr1.2:55310831-55310850 MS.gene32061:CDS 40.0%
TGCAAGAAACATAAAGAGGG+TGG - chr1.2:55310619-55310638 MS.gene32061:CDS 40.0%
TGTCTTGGATTTGTGAATGG+TGG - chr1.2:55310797-55310816 MS.gene32061:CDS 40.0%
TTTCACGCCGATGAAATACT+CGG + chr1.2:55310438-55310457 None:intergenic 40.0%
! TCTTCCATCTCAACTAGCTT+CGG - chr1.2:55310229-55310248 MS.gene32061:CDS 40.0%
! TTTTGTGACTGGGAGAACTA+GGG + chr1.2:55309763-55309782 None:intergenic 40.0%
!! AAGTTGGTGCACATGAGAAT+AGG + chr1.2:55310145-55310164 None:intergenic 40.0%
!! GAAGCTTCTAAAACGGTGTA+AGG + chr1.2:55310557-55310576 None:intergenic 40.0%
AAACTGCGACATTTCGTCCT+GGG + chr1.2:55310115-55310134 None:intergenic 45.0%
AAAGCAGAAGCTGTGCTAAC+AGG + chr1.2:55310395-55310414 None:intergenic 45.0%
AAAGCCGAAGCTAGTTGAGA+TGG + chr1.2:55310236-55310255 None:intergenic 45.0%
AATGTGACTGGGACACGATT+GGG - chr1.2:55310674-55310693 MS.gene32061:CDS 45.0%
ACAGCTTCTGCTTTCTCACA+AGG - chr1.2:55310401-55310420 MS.gene32061:CDS 45.0%
AGTTTGTCGTCCAAAGAGCA+AGG + chr1.2:55310918-55310937 None:intergenic 45.0%
ATCTCGGTGGCCAATTTCTA+TGG - chr1.2:55309859-55309878 MS.gene32061:CDS 45.0%
CCTTCAGCCGAGTATTTCAT+CGG - chr1.2:55310428-55310447 MS.gene32061:CDS 45.0%
GGTTGAAGCCATTAGAGGAT+TGG + chr1.2:55310094-55310113 None:intergenic 45.0%
GTTCTCCCAGTCACAAAAGA+TGG - chr1.2:55309765-55309784 MS.gene32061:CDS 45.0%
TAATGTGACTGGGACACGAT+TGG - chr1.2:55310673-55310692 MS.gene32061:CDS 45.0%
TCTATAGACAACAACGGCGT+TGG - chr1.2:55310506-55310525 MS.gene32061:CDS 45.0%
TCTCGGTGGCCAATTTCTAT+GGG - chr1.2:55309860-55309879 MS.gene32061:CDS 45.0%
TGTTTCTCATCACCAAAGCC+TGG - chr1.2:55309978-55309997 MS.gene32061:CDS 45.0%
TTATTCGGGGATGGACCTTA+TGG - chr1.2:55310308-55310327 MS.gene32061:CDS 45.0%
! CTTTTGTGACTGGGAGAACT+AGG + chr1.2:55309764-55309783 None:intergenic 45.0%
! GGATTTAGCTCCTTGCTCTT+TGG - chr1.2:55310905-55310924 MS.gene32061:CDS 45.0%
! TAGCGCCATCTTTTGTGACT+GGG + chr1.2:55309773-55309792 None:intergenic 45.0%
!! GGAGTTGTTTTATTCGGGGA+TGG - chr1.2:55310299-55310318 MS.gene32061:CDS 45.0%
!! TGTGATTGGAGGGTATCAGT+TGG - chr1.2:55310841-55310860 MS.gene32061:CDS 45.0%
AGATCGACGATGACGTTCAG+TGG + chr1.2:55309843-55309862 None:intergenic 50.0%
ATCTTCAGCAAGTTCACCGC+TGG + chr1.2:55310060-55310079 None:intergenic 50.0%
ATGACGTTCAGTGGGACTAG+TGG + chr1.2:55309834-55309853 None:intergenic 50.0%
CCGATGAAATACTCGGCTGA+AGG + chr1.2:55310431-55310450 None:intergenic 50.0%
CTGAACGTCATCGTCGATCT+CGG - chr1.2:55309843-55309862 MS.gene32061:CDS 50.0%
GAAACAATCACCACAGCCGT+CGG + chr1.2:55309964-55309983 None:intergenic 50.0%
GAAACTGCGACATTTCGTCC+TGG + chr1.2:55310116-55310135 None:intergenic 50.0%
GATCGACGATGACGTTCAGT+GGG + chr1.2:55309842-55309861 None:intergenic 50.0%
TCCTCTAATGGCTTCAACCC+AGG - chr1.2:55310095-55310114 MS.gene32061:CDS 50.0%
! ACTGGGAGAACTAGGGCTTT+TGG + chr1.2:55309756-55309775 None:intergenic 50.0%
! ATAGACAACAACGGCGTTGG+TGG - chr1.2:55310509-55310528 MS.gene32061:CDS 50.0%
! GTAGCGCCATCTTTTGTGAC+TGG + chr1.2:55309774-55309793 None:intergenic 50.0%
! TCCTGGGTTGAAGCCATTAG+AGG + chr1.2:55310099-55310118 None:intergenic 50.0%
! TGACGTTCAGTGGGACTAGT+GGG + chr1.2:55309833-55309852 None:intergenic 50.0%
! TTGTTGTTGCAGCCAGGCTT+TGG + chr1.2:55309993-55310012 None:intergenic 50.0%
!! GATTGGAGGGTATCAGTTGG+AGG - chr1.2:55310844-55310863 MS.gene32061:CDS 50.0%
!! GTGTGGGTGATGGAATTGTC+CGG + chr1.2:55310032-55310051 None:intergenic 50.0%
!! TGTGGGTGATGGAATTGTCC+GGG + chr1.2:55310031-55310050 None:intergenic 50.0%
AACGTCATCGTCGATCTCGG+TGG - chr1.2:55309846-55309865 MS.gene32061:CDS 55.0%
ATCACCACAGCCGTCGGAAT+TGG + chr1.2:55309958-55309977 None:intergenic 55.0%
GGACTTGCAAGTGGTGCTTC+TGG - chr1.2:55310176-55310195 MS.gene32061:CDS 55.0%
GGCTGCAACAACAACACCTG+TGG - chr1.2:55309999-55310018 MS.gene32061:CDS 55.0%
TGCAAGTGGTGCTTCTGGCA+TGG - chr1.2:55310181-55310200 MS.gene32061:CDS 55.0%
TTCAGCAAGTTCACCGCTGG+TGG + chr1.2:55310057-55310076 None:intergenic 55.0%
! GACGTTCAGTGGGACTAGTG+GGG + chr1.2:55309832-55309851 None:intergenic 55.0%
! GTGGGACTAGTGGGGTTCTT+TGG + chr1.2:55309824-55309843 None:intergenic 55.0%
!! CAGGTGTTGTTGTTGCAGCC+AGG + chr1.2:55309999-55310018 None:intergenic 55.0%
!! GGAATTGGCGAGGGAGCATT+GGG + chr1.2:55309943-55309962 None:intergenic 55.0%
!! GTGGGTGATGGAATTGTCCG+GGG + chr1.2:55310030-55310049 None:intergenic 55.0%
ACAGCCGTCGGAATTGGCGA+GGG + chr1.2:55309952-55309971 None:intergenic 60.0%
AGCAAGTTCACCGCTGGTGG+CGG + chr1.2:55310054-55310073 None:intergenic 60.0%
AGTGGTGCTTCTGGCATGGC+TGG - chr1.2:55310185-55310204 MS.gene32061:CDS 60.0%
ATTGTCCGGGGTGACACCAC+AGG + chr1.2:55310018-55310037 None:intergenic 60.0%
TGCTCCCTCGCCAATTCCGA+CGG - chr1.2:55309945-55309964 MS.gene32061:CDS 60.0%
! CGGAATTGGCGAGGGAGCAT+TGG + chr1.2:55309944-55309963 None:intergenic 60.0%
!! CAACACCTGTGGTGTCACCC+CGG - chr1.2:55310010-55310029 MS.gene32061:CDS 60.0%
!! GCTTCTGGCATGGCTGGTCT+TGG - chr1.2:55310191-55310210 MS.gene32061:CDS 60.0%
ACACCGTGCAGGGCGGTATG+TGG + chr1.2:55309913-55309932 None:intergenic 65.0%
ATCACCCACACCGCCACCAG+CGG - chr1.2:55310041-55310060 MS.gene32061:CDS 65.0%
CACAGCCGTCGGAATTGGCG+AGG + chr1.2:55309953-55309972 None:intergenic 65.0%
CCTCCACATACCGCCCTGCA+CGG - chr1.2:55309907-55309926 MS.gene32061:CDS 65.0%
CTCGCCAATTCCGACGGCTG+TGG - chr1.2:55309951-55309970 MS.gene32061:CDS 65.0%
!! TTCACCGCTGGTGGCGGTGT+GGG + chr1.2:55310048-55310067 None:intergenic 65.0%
CCGTGCAGGGCGGTATGTGG+AGG + chr1.2:55309910-55309929 None:intergenic 70.0%
CTGAGCGACACCGTGCAGGG+CGG + chr1.2:55309920-55309939 None:intergenic 70.0%
GGGCTGAGCGACACCGTGCA+GGG + chr1.2:55309923-55309942 None:intergenic 70.0%
TGGGCTGAGCGACACCGTGC+AGG + chr1.2:55309924-55309943 None:intergenic 70.0%
!! GCTGGTGGCGGTGTGGGTGA+TGG + chr1.2:55310042-55310061 None:intergenic 70.0%
!! GTTCACCGCTGGTGGCGGTG+TGG + chr1.2:55310049-55310068 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 55309669 55310973 55309669 ID=MS.gene32061
chr1.2 mRNA 55309669 55310973 55309669 ID=MS.gene32061.t1;Parent=MS.gene32061
chr1.2 exon 55309669 55310973 55309669 ID=MS.gene32061.t1.exon1;Parent=MS.gene32061.t1
chr1.2 CDS 55309669 55310973 55309669 ID=cds.MS.gene32061.t1;Parent=MS.gene32061.t1
Gene Sequence

>MS.gene32061

ATGGCTTCCAATTTTCAACACTTCATTACAACCCTACTCCTATTTTTCTTCATTTCACCCACTTTCTCTCAACAATCTTTCAGGCCAAAAGCCCTAGTTCTCCCAGTCACAAAAGATGGCGCTACAACAAACCAATACAAAGCTCAAATCAACCAAAGAACCCCACTAGTCCCACTGAACGTCATCGTCGATCTCGGTGGCCAATTTCTATGGGTTGATTGTGAAAACAAGTACATTTCCTCCACATACCGCCCTGCACGGTGTCGCTCAGCCCAATGCTCCCTCGCCAATTCCGACGGCTGTGGTGATTGTTTCTCATCACCAAAGCCTGGCTGCAACAACAACACCTGTGGTGTCACCCCGGACAATTCCATCACCCACACCGCCACCAGCGGTGAACTTGCTGAAGATGTTTTATCAATCCAATCCTCTAATGGCTTCAACCCAGGACGAAATGTCGCAGTTTCACGCTTCCTATTCTCATGTGCACCAACTTTCTTACTAAAAGGACTTGCAAGTGGTGCTTCTGGCATGGCTGGTCTTGGAAGAACCAAAATTGCTCTTCCATCTCAACTAGCTTCGGCTTTTAGCTTCGATAGAAAATTCGCTATTTGTCTTTCCTCTTCAAAGGGAGTTGTTTTATTCGGGGATGGACCTTATGGTTTTCTTCCCAACATTGTTCTTGATTCAGACTCGCTCACATACACACCGCTTTTGATCAATCCTGTTAGCACAGCTTCTGCTTTCTCACAAGGTCAGCCTTCAGCCGAGTATTTCATCGGCGTGAAAACAATAAAAATCGATGAAAAAGTTGTTTCACTTAACTCAAGTTTGTTTTCTATAGACAACAACGGCGTTGGTGGAACAAAGATTAGTACGGTTGATCCTTACACCGTTTTAGAAGCTTCTATTTACAAAGCAGTTACAGACGCTTTCGTGAAAGCGTCTGCTGCAAGAAACATAAAGAGGGTGGATTCGGTTGCGCCTTTTGAATTTTGTTACACTAATGTGACTGGGACACGATTGGGTGCAGCTGTGCCTACTATTGAGCTCTTTTTGCAAAATGAAAATGTGGTTTGGAGGATATTTGGTGCAAATTCAATGGTGAATATAAATGATGAGGTTTTGTGTCTTGGATTTGTGAATGGTGGTAAGAATACAAGGACTTCAATTGTGATTGGAGGGTATCAGTTGGAGGATAATCTTGTGCAATTTGATATGGCTGCTTCTAAATTGGGATTTAGCTCCTTGCTCTTTGGACGACAAACTACTTGTTCTAACTTCAACTTTACCTCCACTGCCTAG
Protein sequence

>MS.gene32061.t1

MASNFQHFITTLLLFFFISPTFSQQSFRPKALVLPVTKDGATTNQYKAQINQRTPLVPLNVIVDLGGQFLWVDCENKYISSTYRPARCRSAQCSLANSDGCGDCFSSPKPGCNNNTCGVTPDNSITHTATSGELAEDVLSIQSSNGFNPGRNVAVSRFLFSCAPTFLLKGLASGASGMAGLGRTKIALPSQLASAFSFDRKFAICLSSSKGVVLFGDGPYGFLPNIVLDSDSLTYTPLLINPVSTASAFSQGQPSAEYFIGVKTIKIDEKVVSLNSSLFSIDNNGVGGTKISTVDPYTVLEASIYKAVTDAFVKASAARNIKRVDSVAPFEFCYTNVTGTRLGAAVPTIELFLQNENVVWRIFGANSMVNINDEVLCLGFVNGGKNTRTSIVIGGYQLEDNLVQFDMAASKLGFSSLLFGRQTTCSNFNFTSTA