Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33030.t1 | XP_024631073.1 | 96.4 | 563 | 20 | 0 | 33 | 595 | 1 | 563 | 0.00E+00 | 1095.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33030.t1 | Q8BVH9 | 43.7 | 254 | 138 | 2 | 48 | 297 | 14 | 266 | 1.9e-51 | 205.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33030.t1 | A0A396JIH7 | 96.4 | 563 | 20 | 0 | 33 | 595 | 1 | 563 | 0.0e+00 | 1095.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049824 | MS.gene33030 | 0.807505 | 4.89E-50 | -1.69E-46 |
| MS.gene056091 | MS.gene33030 | 0.803771 | 2.97E-49 | -1.69E-46 |
| MS.gene056403 | MS.gene33030 | 0.824111 | 9.64E-54 | -1.69E-46 |
| MS.gene056605 | MS.gene33030 | 0.820437 | 6.86E-53 | -1.69E-46 |
| MS.gene06001 | MS.gene33030 | 0.802915 | 4.47E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene33030 | MS.gene78481 | PPI |
| MS.gene33030 | MS.gene041599 | PPI |
| MS.gene33030 | MS.gene035362 | PPI |
| MS.gene33030 | MS.gene041598 | PPI |
| MS.gene33030 | MS.gene77822 | PPI |
| MS.gene33030 | MS.gene035363 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33030.t1 | MTR_1g015770 | 93.684 | 570 | 29 | 1 | 33 | 595 | 1 | 570 | 0.0 | 1083 |
| MS.gene33030.t1 | MTR_1g079800 | 32.963 | 270 | 129 | 7 | 79 | 297 | 53 | 321 | 1.74e-32 | 128 |
| MS.gene33030.t1 | MTR_1g079800 | 32.296 | 257 | 123 | 6 | 91 | 297 | 89 | 344 | 8.94e-29 | 118 |
| MS.gene33030.t1 | MTR_8g056070 | 82.500 | 40 | 7 | 0 | 467 | 506 | 32 | 71 | 1.68e-14 | 70.1 |
| MS.gene33030.t1 | MTR_3g036280 | 78.947 | 38 | 8 | 0 | 469 | 506 | 24 | 61 | 1.37e-13 | 66.2 |
| MS.gene33030.t1 | MTR_3g045720 | 29.252 | 147 | 96 | 4 | 354 | 495 | 35 | 178 | 1.00e-12 | 67.4 |
| MS.gene33030.t1 | MTR_7g032210 | 75.000 | 40 | 10 | 0 | 467 | 506 | 67 | 106 | 1.92e-12 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33030.t1 | AT2G26200 | 64.791 | 551 | 187 | 4 | 51 | 595 | 16 | 565 | 0.0 | 730 |
| MS.gene33030.t1 | AT2G26200 | 64.438 | 329 | 106 | 4 | 39 | 358 | 1 | 327 | 7.69e-148 | 431 |
| MS.gene33030.t1 | AT1G54650 | 34.524 | 252 | 140 | 4 | 70 | 297 | 43 | 293 | 1.96e-36 | 138 |
| MS.gene33030.t1 | AT1G54650 | 34.524 | 252 | 140 | 4 | 70 | 297 | 63 | 313 | 3.02e-36 | 138 |
| MS.gene33030.t1 | AT2G26810 | 31.214 | 173 | 108 | 5 | 385 | 552 | 59 | 225 | 3.61e-17 | 82.0 |
| MS.gene33030.t1 | AT2G26810 | 31.214 | 173 | 108 | 5 | 385 | 552 | 59 | 225 | 3.61e-17 | 82.0 |
| MS.gene33030.t1 | AT2G26810 | 38.261 | 115 | 65 | 3 | 385 | 495 | 59 | 171 | 3.86e-15 | 75.1 |
| MS.gene33030.t1 | AT2G26810 | 38.261 | 115 | 65 | 3 | 385 | 495 | 59 | 171 | 3.86e-15 | 75.1 |
| MS.gene33030.t1 | AT2G26810 | 38.261 | 115 | 65 | 3 | 385 | 495 | 59 | 171 | 3.86e-15 | 75.1 |
| MS.gene33030.t1 | AT2G26810 | 38.261 | 115 | 65 | 3 | 385 | 495 | 67 | 179 | 4.54e-15 | 75.1 |
| MS.gene33030.t1 | AT2G26810 | 38.261 | 115 | 65 | 3 | 385 | 495 | 67 | 179 | 4.54e-15 | 75.1 |
| MS.gene33030.t1 | AT1G54650 | 34.906 | 106 | 66 | 1 | 70 | 172 | 63 | 168 | 1.30e-12 | 67.8 |
Find 111 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAATATTGTTTCCGGAAAA+AGG | 0.103649 | 1.1:-6247871 | MS.gene33030:CDS |
| GCAGATGCATGAAGACTTTA+AGG | 0.160121 | 1.1:-6251632 | MS.gene33030:intron |
| TGTTATTGACAATTGGTTTA+TGG | 0.179744 | 1.1:-6247381 | MS.gene33030:CDS |
| AGGAGGAAAGAAAGTAATTT+TGG | 0.191683 | 1.1:-6252871 | MS.gene33030:CDS |
| CTTTCAGCTGCATCACAATT+TGG | 0.194547 | 1.1:-6247458 | MS.gene33030:CDS |
| ATTTATTCCAAACCAAATTC+TGG | 0.197214 | 1.1:-6254772 | MS.gene33030:CDS |
| ACAAATTTATTCAAAGAAAA+TGG | 0.213631 | 1.1:-6249000 | MS.gene33030:CDS |
| ACAAAGACCACTTCAGTTTC+AGG | 0.224017 | 1.1:+6254904 | None:intergenic |
| GAAAATCCCAATATTGTTTC+CGG | 0.249931 | 1.1:-6247878 | MS.gene33030:CDS |
| AGACGTTTCAGGCGAATTTC+CGG | 0.250447 | 1.1:+6247409 | None:intergenic |
| AAGGGTGAGACCCCAGAATT+TGG | 0.265459 | 1.1:+6254760 | None:intergenic |
| ATAACACTTCGAGACGTTTC+AGG | 0.273056 | 1.1:+6247398 | None:intergenic |
| AAAGAGAAGCTAAGAAATAT+TGG | 0.275974 | 1.1:-6254626 | MS.gene33030:CDS |
| ATTCGCCTCAGCTTCTTTAC+TGG | 0.282944 | 1.1:+6248757 | None:intergenic |
| AATATGTTTAATGCACTATT+AGG | 0.285579 | 1.1:+6247335 | None:intergenic |
| TTTCAGCTGCATCACAATTT+GGG | 0.310864 | 1.1:-6247457 | MS.gene33030:CDS |
| ATTCTGGGGTCTCACCCTTT+TGG | 0.312133 | 1.1:-6254756 | MS.gene33030:CDS |
| TGATGCTATCACAGGAAATA+TGG | 0.318795 | 1.1:+6252347 | None:intergenic |
| ATAAAGGAGATGAGTGATAG+AGG | 0.329154 | 1.1:-6247647 | MS.gene33030:CDS |
| CATCAAACCAGTTGATTTGC+AGG | 0.330099 | 1.1:+6247940 | None:intergenic |
| TTAATATTCTGCAAAACAAT+AGG | 0.332969 | 1.1:+6251314 | None:intergenic |
| GTGGGAATCGGCCCGATTAA+TGG | 0.336825 | 1.1:-6247915 | MS.gene33030:CDS |
| CTTGATGGTGATAACAATAT+TGG | 0.341012 | 1.1:-6248731 | MS.gene33030:CDS |
| CATCGACACGGCGAAAGATA+TGG | 0.341313 | 1.1:+6247492 | None:intergenic |
| GATCTCACGGCAATGGCATC+AGG | 0.356294 | 1.1:-6254832 | MS.gene33030:CDS |
| GAAAACTAACCTTGTCTTTG+TGG | 0.362479 | 1.1:+6254586 | None:intergenic |
| TTTATATAGGAGAGATTCTC+TGG | 0.370599 | 1.1:-6249190 | MS.gene33030:intron |
| CTCGAAGTGTTATTGACAAT+TGG | 0.375056 | 1.1:-6247388 | MS.gene33030:CDS |
| CAAGGACGGAAGCCATTAAT+CGG | 0.393239 | 1.1:+6247903 | None:intergenic |
| TTGACATGTCTGAGGGAGTC+GGG | 0.399247 | 1.1:-6248661 | MS.gene33030:CDS |
| TTTATTCCAAACCAAATTCT+GGG | 0.404687 | 1.1:-6254771 | MS.gene33030:CDS |
| CTCACCACGAGCTATTGAAT+TGG | 0.405150 | 1.1:-6252292 | MS.gene33030:CDS |
| GGAGTCGGGGCTGACATGTT+TGG | 0.408447 | 1.1:-6248647 | MS.gene33030:CDS |
| TTTCAAAGACCGGCATTACT+TGG | 0.412980 | 1.1:-6253020 | MS.gene33030:CDS |
| ACGAGCTATTGAATTGGTTA+AGG | 0.421963 | 1.1:-6252286 | MS.gene33030:intron |
| TGAGACCCCAGAATTTGGTT+TGG | 0.422537 | 1.1:+6254765 | None:intergenic |
| CGCAGCAGCACATATCCATT+TGG | 0.435282 | 1.1:+6251179 | None:intergenic |
| CATGCATAAACAAAAGCATC+TGG | 0.435659 | 1.1:+6252321 | None:intergenic |
| AAGAGAAGCTAAGAAATATT+GGG | 0.435875 | 1.1:-6254625 | MS.gene33030:CDS |
| CAACATACCTGCAAATCAAC+TGG | 0.438434 | 1.1:-6247947 | MS.gene33030:CDS |
| TTGGAGTTAGGGTGTGGCAG+TGG | 0.441103 | 1.1:-6247845 | MS.gene33030:CDS |
| TTTCTCCATTTGCAGCCAAA+TGG | 0.443656 | 1.1:-6251194 | MS.gene33030:intron |
| TGGTTTGATGCTGTGGGAAT+CGG | 0.444115 | 1.1:-6247927 | MS.gene33030:CDS |
| GTTTGCTGGTTTCAGGCGTT+GGG | 0.449293 | 1.1:-6248819 | MS.gene33030:intron |
| GCATTACTTGGATAAAGAAT+GGG | 0.455655 | 1.1:-6253008 | MS.gene33030:CDS |
| GGCATTACTTGGATAAAGAA+TGG | 0.457337 | 1.1:-6253009 | MS.gene33030:CDS |
| ATTATGGAGATCAACCTTAG+AGG | 0.457883 | 1.1:-6248007 | MS.gene33030:CDS |
| CAAAGACCACTTCAGTTTCA+GGG | 0.463569 | 1.1:+6254905 | None:intergenic |
| AGCTTCTTTACTGGAAGAGT+TGG | 0.466430 | 1.1:+6248766 | None:intergenic |
| AGGGTAGGTACCTCTCCAAA+AGG | 0.473644 | 1.1:+6254741 | None:intergenic |
| GCTGAGGCGAATCATCTTGA+TGG | 0.475133 | 1.1:-6248746 | MS.gene33030:CDS |
| TTTACTGAACAGGTTGGGTG+TGG | 0.479098 | 1.1:-6252381 | MS.gene33030:intron |
| CTGCTGCGAGACTATGCTAC+TGG | 0.479610 | 1.1:-6251164 | MS.gene33030:CDS |
| CTTGCCTTCCGACGAGTATG+AGG | 0.495769 | 1.1:-6248621 | MS.gene33030:intron |
| TTCTGTTGATATTGTTACAA+TGG | 0.496744 | 1.1:-6251542 | MS.gene33030:intron |
| AGATCACATAGAAAGCATAA+AGG | 0.499104 | 1.1:-6247663 | MS.gene33030:CDS |
| CACGTTAATGCTTGATCTCA+CGG | 0.501128 | 1.1:-6254845 | MS.gene33030:CDS |
| GAGTTAGGGTGTGGCAGTGG+TGG | 0.504655 | 1.1:-6247842 | MS.gene33030:CDS |
| TGGAGATCAACCTTAGAGGC+TGG | 0.507549 | 1.1:-6248003 | MS.gene33030:CDS |
| GAACAGGTTGGGTGTGGAGC+TGG | 0.509594 | 1.1:-6252375 | MS.gene33030:intron |
| CAGCGACAAAAGCGTGAACA+TGG | 0.510092 | 1.1:+6251605 | None:intergenic |
| TATGTTATCTGCAGTGTCCC+CGG | 0.526619 | 1.1:-6251345 | MS.gene33030:CDS |
| ATTTGCCACTTCAAACTCAA+AGG | 0.532302 | 1.1:-6254801 | MS.gene33030:CDS |
| CTGAAACACAAAATACAGCT+TGG | 0.541542 | 1.1:+6248795 | None:intergenic |
| AATCTCTTTGCACAAATCAT+CGG | 0.542409 | 1.1:+6251573 | None:intergenic |
| TGCAACCTTTGAGTTTGAAG+TGG | 0.544850 | 1.1:+6254796 | None:intergenic |
| AAGAAGTTGAGAACCGCTCA+AGG | 0.548248 | 1.1:-6248945 | MS.gene33030:CDS |
| AATCAACTGGTTTGATGCTG+TGG | 0.549620 | 1.1:-6247934 | MS.gene33030:CDS |
| AGGGTATTGGAGTTAGGGTG+TGG | 0.549969 | 1.1:-6247851 | MS.gene33030:CDS |
| GTAACAATATCAACAGAAGA+TGG | 0.550718 | 1.1:+6251547 | None:intergenic |
| CAGTTGATTTGCAGGTATGT+TGG | 0.552753 | 1.1:+6247948 | None:intergenic |
| GTAGCTAGTTTAGTCAGCAA+TGG | 0.555650 | 1.1:+6247710 | None:intergenic |
| AAACATATTGCTCTTCTGCA+TGG | 0.557086 | 1.1:-6247321 | MS.gene33030:CDS |
| ATTCTTTATCCAAGTAATGC+CGG | 0.560199 | 1.1:+6253011 | None:intergenic |
| ATCTGTAGCAACTACTCGAT+CGG | 0.561693 | 1.1:+6247790 | None:intergenic |
| TGCTACTGGAGACCTTGCTC+AGG | 0.567273 | 1.1:-6251150 | MS.gene33030:intron |
| TTAGTAGACAAGTGGCCTAC+CGG | 0.568314 | 1.1:-6247428 | MS.gene33030:CDS |
| CAAGGGAGTTGATAATGAAA+CGG | 0.570171 | 1.1:-6248927 | MS.gene33030:intron |
| AATGCTTGATCTCACGGCAA+TGG | 0.571311 | 1.1:-6254839 | MS.gene33030:CDS |
| TCTGGATATGATGCTATCAC+AGG | 0.575758 | 1.1:+6252339 | None:intergenic |
| ATTGACATGTCTGAGGGAGT+CGG | 0.576361 | 1.1:-6248662 | MS.gene33030:CDS |
| AAGGACGGAAGCCATTAATC+GGG | 0.578586 | 1.1:+6247904 | None:intergenic |
| TAAAGGAGATGAGTGATAGA+GGG | 0.579905 | 1.1:-6247646 | MS.gene33030:CDS |
| GGGTAGGTACCTCTCCAAAA+GGG | 0.580724 | 1.1:+6254742 | None:intergenic |
| ATCTCACGGCAATGGCATCA+GGG | 0.581718 | 1.1:-6254831 | MS.gene33030:CDS |
| CTCTTCCAGTAAAGAAGCTG+AGG | 0.582410 | 1.1:-6248762 | MS.gene33030:CDS |
| TCATTATCAACTCCCTTGAG+CGG | 0.584655 | 1.1:+6248932 | None:intergenic |
| GGTTTATATTAGTAGACAAG+TGG | 0.593320 | 1.1:-6247436 | MS.gene33030:CDS |
| AGAGGGTTCAATGTCATCAT+CGG | 0.597470 | 1.1:-6247629 | MS.gene33030:CDS |
| GATCGAGTAGTTGCTACAGA+TGG | 0.598212 | 1.1:-6247788 | MS.gene33030:CDS |
| ATATGGCAAAGAATAAGTAC+AGG | 0.601647 | 1.1:+6247509 | None:intergenic |
| CTCTGTAATTGACATGTCTG+AGG | 0.603915 | 1.1:-6248669 | MS.gene33030:CDS |
| ATCAACTGGTTTGATGCTGT+GGG | 0.608480 | 1.1:-6247933 | MS.gene33030:CDS |
| TTATTCCAAACCAAATTCTG+GGG | 0.617390 | 1.1:-6254770 | MS.gene33030:CDS |
| GATAAACCCTGAAACTGAAG+TGG | 0.621986 | 1.1:-6254911 | MS.gene33030:CDS |
| TGACATGTCTGAGGGAGTCG+GGG | 0.622122 | 1.1:-6248660 | MS.gene33030:CDS |
| ATAGGAGAGATTCTCTGGCA+AGG | 0.623934 | 1.1:-6249185 | MS.gene33030:intron |
| CAACAAAATCAACCTGAGCA+AGG | 0.630816 | 1.1:+6251138 | None:intergenic |
| TCTCACGGCAATGGCATCAG+GGG | 0.631546 | 1.1:-6254830 | MS.gene33030:CDS |
| CTACAAACACCACAAAGACA+AGG | 0.634893 | 1.1:-6254595 | MS.gene33030:intron |
| CTTACCTCATACTCGTCGGA+AGG | 0.644138 | 1.1:+6248617 | None:intergenic |
| AGAAGTTGAGAACCGCTCAA+GGG | 0.645207 | 1.1:-6248944 | MS.gene33030:CDS |
| GTTTCAGGCGAATTTCCGGT+AGG | 0.649486 | 1.1:+6247413 | None:intergenic |
| TGGTGATAACAATATTGGCA+CGG | 0.662682 | 1.1:-6248726 | MS.gene33030:CDS |
| AAGCCTTACCTCATACTCGT+CGG | 0.662998 | 1.1:+6248613 | None:intergenic |
| AGCGACAAAAGCGTGAACAT+GGG | 0.663872 | 1.1:+6251606 | None:intergenic |
| GCAACGTTGGTTCATCGACA+CGG | 0.677877 | 1.1:+6247480 | None:intergenic |
| CATTACTTGGATAAAGAATG+GGG | 0.679169 | 1.1:-6253007 | MS.gene33030:CDS |
| TCTGTAATTGACATGTCTGA+GGG | 0.683888 | 1.1:-6248668 | MS.gene33030:CDS |
| GATGCAGCTGAAAGCAACGT+TGG | 0.684511 | 1.1:+6247467 | None:intergenic |
| TTAACCAATTCAATAGCTCG+TGG | 0.686527 | 1.1:+6252288 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 6247316 | 6254956 | 6247316 | ID=MS.gene33030 |
| chr1.1 | mRNA | 6247316 | 6254956 | 6247316 | ID=MS.gene33030.t1;Parent=MS.gene33030 |
| chr1.1 | exon | 6254752 | 6254956 | 6254752 | ID=MS.gene33030.t1.exon1;Parent=MS.gene33030.t1 |
| chr1.1 | CDS | 6254752 | 6254956 | 6254752 | ID=cds.MS.gene33030.t1;Parent=MS.gene33030.t1 |
| chr1.1 | exon | 6254596 | 6254657 | 6254596 | ID=MS.gene33030.t1.exon2;Parent=MS.gene33030.t1 |
| chr1.1 | CDS | 6254596 | 6254657 | 6254596 | ID=cds.MS.gene33030.t1;Parent=MS.gene33030.t1 |
| chr1.1 | exon | 6252994 | 6253044 | 6252994 | ID=MS.gene33030.t1.exon3;Parent=MS.gene33030.t1 |
| chr1.1 | CDS | 6252994 | 6253044 | 6252994 | ID=cds.MS.gene33030.t1;Parent=MS.gene33030.t1 |
| chr1.1 | exon | 6252869 | 6252895 | 6252869 | ID=MS.gene33030.t1.exon4;Parent=MS.gene33030.t1 |
| chr1.1 | CDS | 6252869 | 6252895 | 6252869 | ID=cds.MS.gene33030.t1;Parent=MS.gene33030.t1 |
| chr1.1 | exon | 6252287 | 6252391 | 6252287 | ID=MS.gene33030.t1.exon5;Parent=MS.gene33030.t1 |
| chr1.1 | CDS | 6252287 | 6252391 | 6252287 | ID=cds.MS.gene33030.t1;Parent=MS.gene33030.t1 |
| chr1.1 | exon | 6251543 | 6251650 | 6251543 | ID=MS.gene33030.t1.exon6;Parent=MS.gene33030.t1 |
| chr1.1 | CDS | 6251543 | 6251650 | 6251543 | ID=cds.MS.gene33030.t1;Parent=MS.gene33030.t1 |
| chr1.1 | exon | 6251301 | 6251372 | 6251301 | ID=MS.gene33030.t1.exon7;Parent=MS.gene33030.t1 |
| chr1.1 | CDS | 6251301 | 6251372 | 6251301 | ID=cds.MS.gene33030.t1;Parent=MS.gene33030.t1 |
| chr1.1 | exon | 6251151 | 6251201 | 6251151 | ID=MS.gene33030.t1.exon8;Parent=MS.gene33030.t1 |
| chr1.1 | CDS | 6251151 | 6251201 | 6251151 | ID=cds.MS.gene33030.t1;Parent=MS.gene33030.t1 |
| chr1.1 | exon | 6249141 | 6249203 | 6249141 | ID=MS.gene33030.t1.exon9;Parent=MS.gene33030.t1 |
| chr1.1 | CDS | 6249141 | 6249203 | 6249141 | ID=cds.MS.gene33030.t1;Parent=MS.gene33030.t1 |
| chr1.1 | exon | 6248928 | 6249052 | 6248928 | ID=MS.gene33030.t1.exon10;Parent=MS.gene33030.t1 |
| chr1.1 | CDS | 6248928 | 6249052 | 6248928 | ID=cds.MS.gene33030.t1;Parent=MS.gene33030.t1 |
| chr1.1 | exon | 6248622 | 6248826 | 6248622 | ID=MS.gene33030.t1.exon11;Parent=MS.gene33030.t1 |
| chr1.1 | CDS | 6248622 | 6248826 | 6248622 | ID=cds.MS.gene33030.t1;Parent=MS.gene33030.t1 |
| chr1.1 | exon | 6247316 | 6248029 | 6247316 | ID=MS.gene33030.t1.exon12;Parent=MS.gene33030.t1 |
| chr1.1 | CDS | 6247316 | 6248029 | 6247316 | ID=cds.MS.gene33030.t1;Parent=MS.gene33030.t1 |
| Gene Sequence |
| Protein sequence |
