Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33494.t1 | XP_003604643.1 | 88.5 | 104 | 12 | 0 | 1 | 104 | 130 | 233 | 3.40E-48 | 200.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33494.t1 | P26205 | 57.7 | 130 | 30 | 2 | 1 | 105 | 114 | 243 | 1.8e-37 | 156.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33494.t1 | A8TVQ0 | 88.5 | 104 | 12 | 0 | 1 | 104 | 130 | 233 | 2.5e-48 | 200.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04932 | MS.gene33494 | 0.828341 | 9.49E-55 | -1.69E-46 |
| MS.gene05240 | MS.gene33494 | 0.839741 | 1.32E-57 | -1.69E-46 |
| MS.gene052702 | MS.gene33494 | 0.829828 | 4.14E-55 | -1.69E-46 |
| MS.gene054181 | MS.gene33494 | 0.814075 | 1.85E-51 | -1.69E-46 |
| MS.gene054277 | MS.gene33494 | 0.805381 | 1.37E-49 | -1.69E-46 |
| MS.gene054290 | MS.gene33494 | 0.801747 | 7.78E-49 | -1.69E-46 |
| MS.gene054742 | MS.gene33494 | 0.817592 | 3.04E-52 | -1.69E-46 |
| MS.gene056590 | MS.gene33494 | 0.814371 | 1.59E-51 | -1.69E-46 |
| MS.gene056591 | MS.gene33494 | 0.813001 | 3.19E-51 | -1.69E-46 |
| MS.gene056688 | MS.gene33494 | 0.809967 | 1.46E-50 | -1.69E-46 |
| MS.gene056697 | MS.gene33494 | 0.81468 | 1.36E-51 | -1.69E-46 |
| MS.gene056920 | MS.gene33494 | 0.806347 | 8.60E-50 | -1.69E-46 |
| MS.gene057261 | MS.gene33494 | 0.831732 | 1.41E-55 | -1.69E-46 |
| MS.gene057544 | MS.gene33494 | 0.843051 | 1.78E-58 | -1.69E-46 |
| MS.gene058603 | MS.gene33494 | 0.800471 | 1.42E-48 | -1.69E-46 |
| MS.gene059402 | MS.gene33494 | 0.815053 | 1.13E-51 | -1.69E-46 |
| MS.gene059953 | MS.gene33494 | 0.80974 | 1.63E-50 | -1.69E-46 |
| MS.gene059955 | MS.gene33494 | 0.81088 | 9.25E-51 | -1.69E-46 |
| MS.gene060087 | MS.gene33494 | 0.803079 | 4.14E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATATATGCTTCAAGGAATT+TGG | 0.182257 | 4.2:+85850594 | MS.gene33494:CDS |
| GGGGAAGATCCCAATGAAAA+AGG | 0.264707 | 4.2:-85850034 | None:intergenic |
| ATACTCATCTTGTAAGGCCT+GGG | 0.427696 | 4.2:-85850054 | None:intergenic |
| ATCAATGAGCTACTTGCTAA+TGG | 0.429019 | 4.2:+85849918 | MS.gene33494:CDS |
| CAGGCCTTACAAGATGAGTA+TGG | 0.435383 | 4.2:+85850056 | MS.gene33494:CDS |
| CATACTCATCTTGTAAGGCC+TGG | 0.455210 | 4.2:-85850055 | None:intergenic |
| TAGCAAGTAGCTCATTGATC+AGG | 0.461889 | 4.2:-85849914 | None:intergenic |
| ACACTTTGAATACATCAGAT+TGG | 0.462336 | 4.2:+85850661 | MS.gene33494:CDS |
| CTTCGTGGATATATGCTTCA+AGG | 0.473903 | 4.2:+85850587 | MS.gene33494:CDS |
| TTGGAGACAGAGTAAAGCAT+TGG | 0.480355 | 4.2:+85850613 | MS.gene33494:CDS |
| GCCTCCATACTCATCTTGTA+AGG | 0.497866 | 4.2:-85850060 | None:intergenic |
| TGGAGACAGAGTAAAGCATT+GGG | 0.508305 | 4.2:+85850614 | MS.gene33494:CDS |
| CAGCTGCATGAGCAAGAAGT+TGG | 0.509222 | 4.2:-85850701 | None:intergenic |
| GAAGTTGGTTGTGTGTGGCC+TGG | 0.537777 | 4.2:-85850686 | None:intergenic |
| GTATTCAAAGTGTAAGAGAA+TGG | 0.570526 | 4.2:-85850651 | None:intergenic |
| TGTAAAATATGATTCCTGCT+TGG | 0.587277 | 4.2:-85849887 | None:intergenic |
| GAATACATCAGATTGGTACC+AGG | 0.597455 | 4.2:+85850668 | MS.gene33494:CDS |
| AGTGGCGGTGTAAACCAAGC+AGG | 0.626808 | 4.2:+85849873 | MS.gene33494:CDS |
| AGCAAGAAGTTGGTTGTGTG+TGG | 0.636210 | 4.2:-85850691 | None:intergenic |
| TACTCATCTTGTAAGGCCTG+GGG | 0.645812 | 4.2:-85850053 | None:intergenic |
| TATGCAGAAGGAAAACTAAG+TGG | 0.653344 | 4.2:+85849855 | None:intergenic |
| GCAGAAGGAAAACTAAGTGG+CGG | 0.686824 | 4.2:+85849858 | None:intergenic |
| GCCTTACAAGATGAGTATGG+AGG | 0.687554 | 4.2:+85850059 | MS.gene33494:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTCAAGAAATAATAAATTG+AGG | + | chr4.2:85850489-85850508 | MS.gene33494:intron | 15.0% |
| !! | TATGATATACTAATAAAGTA+TGG | - | chr4.2:85849958-85849977 | None:intergenic | 15.0% |
| !! | TTCATAATAATACAAATTGA+TGG | + | chr4.2:85849978-85849997 | MS.gene33494:intron | 15.0% |
| ! | ATTCTAATGTTAGTTAGTTG+TGG | - | chr4.2:85850443-85850462 | None:intergenic | 25.0% |
| ! | TAACTAACTAGTTAGTTAGT+TGG | - | chr4.2:85850383-85850402 | None:intergenic | 25.0% |
| !! | AGAGAAAGATTAGAATGTTT+TGG | + | chr4.2:85850150-85850169 | MS.gene33494:intron | 25.0% |
| !! | TATATGCAGAGATGATTTTA+AGG | + | chr4.2:85850563-85850582 | MS.gene33494:intron | 25.0% |
| AAATCAACTATGTTGTTGGT+TGG | - | chr4.2:85850418-85850437 | None:intergenic | 30.0% | |
| ACACTTTGAATACATCAGAT+TGG | + | chr4.2:85850661-85850680 | MS.gene33494:CDS | 30.0% | |
| ATTGATGGAATTATTGATCC+AGG | + | chr4.2:85849993-85850012 | MS.gene33494:intron | 30.0% | |
| GATATATGCTTCAAGGAATT+TGG | + | chr4.2:85850594-85850613 | MS.gene33494:CDS | 30.0% | |
| GGATAAATCAACTATGTTGT+TGG | - | chr4.2:85850422-85850441 | None:intergenic | 30.0% | |
| GTATTCAAAGTGTAAGAGAA+TGG | - | chr4.2:85850654-85850673 | None:intergenic | 30.0% | |
| TGTAAAATATGATTCCTGCT+TGG | - | chr4.2:85849890-85849909 | None:intergenic | 30.0% | |
| ! | CTTTAGAGAGAAAACTACAA+AGG | + | chr4.2:85850224-85850243 | MS.gene33494:intron | 30.0% |
| !! | ATATGTGACCCTTTTTCATT+GGG | + | chr4.2:85850025-85850044 | MS.gene33494:CDS | 30.0% |
| CAATGAAAAAGGGTCACATA+TGG | - | chr4.2:85850026-85850045 | None:intergenic | 35.0% | |
| CTAGTTAGTTAGTTGGTTAG+TGG | - | chr4.2:85850376-85850395 | None:intergenic | 35.0% | |
| ! | ATCAATGAGCTACTTGCTAA+TGG | + | chr4.2:85849918-85849937 | MS.gene33494:CDS | 35.0% |
| !! | AGATGATTTTAAGGACTTCG+TGG | + | chr4.2:85850572-85850591 | MS.gene33494:CDS | 35.0% |
| !! | CATATGTGACCCTTTTTCAT+TGG | + | chr4.2:85850024-85850043 | MS.gene33494:CDS | 35.0% |
| ATACTCATCTTGTAAGGCCT+GGG | - | chr4.2:85850057-85850076 | None:intergenic | 40.0% | |
| CTTCGTGGATATATGCTTCA+AGG | + | chr4.2:85850587-85850606 | MS.gene33494:CDS | 40.0% | |
| GAATACATCAGATTGGTACC+AGG | + | chr4.2:85850668-85850687 | MS.gene33494:CDS | 40.0% | |
| GGGAAGATCCCAATGAAAAA+GGG | - | chr4.2:85850036-85850055 | None:intergenic | 40.0% | |
| GTCACATATGGTATTTGACC+TGG | - | chr4.2:85850014-85850033 | None:intergenic | 40.0% | |
| TAGCAAGTAGCTCATTGATC+AGG | - | chr4.2:85849917-85849936 | None:intergenic | 40.0% | |
| TTGGAGACAGAGTAAAGCAT+TGG | + | chr4.2:85850613-85850632 | MS.gene33494:CDS | 40.0% | |
| ! | CAACTATGTTGTTGGTTGGT+TGG | - | chr4.2:85850414-85850433 | None:intergenic | 40.0% |
| ! | TGGAGACAGAGTAAAGCATT+GGG | + | chr4.2:85850614-85850633 | MS.gene33494:CDS | 40.0% |
| !! | CTCTCTAAAGTGTGAGTGTA+TGG | - | chr4.2:85850214-85850233 | None:intergenic | 40.0% |
| CAGGCCTTACAAGATGAGTA+TGG | + | chr4.2:85850056-85850075 | MS.gene33494:CDS | 45.0% | |
| CATACTCATCTTGTAAGGCC+TGG | - | chr4.2:85850058-85850077 | None:intergenic | 45.0% | |
| GCCTCCATACTCATCTTGTA+AGG | - | chr4.2:85850063-85850082 | None:intergenic | 45.0% | |
| GCCTTACAAGATGAGTATGG+AGG | + | chr4.2:85850059-85850078 | MS.gene33494:CDS | 45.0% | |
| GGGGAAGATCCCAATGAAAA+AGG | - | chr4.2:85850037-85850056 | None:intergenic | 45.0% | |
| TACTCATCTTGTAAGGCCTG+GGG | - | chr4.2:85850056-85850075 | None:intergenic | 45.0% | |
| ! | AGCAAGAAGTTGGTTGTGTG+TGG | - | chr4.2:85850694-85850713 | None:intergenic | 45.0% |
| ! | TTTTCATTGGGATCTTCCCC+AGG | + | chr4.2:85850037-85850056 | MS.gene33494:CDS | 45.0% |
| AGTGGCGGTGTAAACCAAGC+AGG | + | chr4.2:85849873-85849892 | MS.gene33494:CDS | 55.0% | |
| ! | GAAGTTGGTTGTGTGTGGCC+TGG | - | chr4.2:85850689-85850708 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 85849862 | 85850722 | 85849862 | ID=MS.gene33494 |
| chr4.2 | mRNA | 85849862 | 85850722 | 85849862 | ID=MS.gene33494.t1;Parent=MS.gene33494 |
| chr4.2 | exon | 85849862 | 85849939 | 85849862 | ID=MS.gene33494.t1.exon1;Parent=MS.gene33494.t1 |
| chr4.2 | CDS | 85849862 | 85849939 | 85849862 | ID=cds.MS.gene33494.t1;Parent=MS.gene33494.t1 |
| chr4.2 | exon | 85850015 | 85850102 | 85850015 | ID=MS.gene33494.t1.exon2;Parent=MS.gene33494.t1 |
| chr4.2 | CDS | 85850015 | 85850102 | 85850015 | ID=cds.MS.gene33494.t1;Parent=MS.gene33494.t1 |
| chr4.2 | exon | 85850572 | 85850722 | 85850572 | ID=MS.gene33494.t1.exon3;Parent=MS.gene33494.t1 |
| chr4.2 | CDS | 85850572 | 85850722 | 85850572 | ID=cds.MS.gene33494.t1;Parent=MS.gene33494.t1 |
| Gene Sequence |
| Protein sequence |
