Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34898.t1 | AES99193.1 | 80.4 | 92 | 15 | 1 | 1 | 92 | 1 | 89 | 6.50E-27 | 129.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34898.t1 | G7KG06 | 80.4 | 92 | 15 | 1 | 1 | 92 | 1 | 89 | 4.7e-27 | 129.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050133 | MS.gene34898 | -0.807989 | 3.86E-50 | -1.69E-46 |
| MS.gene05081 | MS.gene34898 | 0.866813 | 2.17E-65 | -1.69E-46 |
| MS.gene051455 | MS.gene34898 | 0.800401 | 1.47E-48 | -1.69E-46 |
| MS.gene051838 | MS.gene34898 | 0.861463 | 1.01E-63 | -1.69E-46 |
| MS.gene052134 | MS.gene34898 | 0.801917 | 7.18E-49 | -1.69E-46 |
| MS.gene052469 | MS.gene34898 | 0.849997 | 2.26E-60 | -1.69E-46 |
| MS.gene052872 | MS.gene34898 | 0.800095 | 1.69E-48 | -1.69E-46 |
| MS.gene053824 | MS.gene34898 | 0.804252 | 2.36E-49 | -1.69E-46 |
| MS.gene054107 | MS.gene34898 | 0.805525 | 1.28E-49 | -1.69E-46 |
| MS.gene054810 | MS.gene34898 | -0.800991 | 1.11E-48 | -1.69E-46 |
| MS.gene054884 | MS.gene34898 | -0.814889 | 1.22E-51 | -1.69E-46 |
| MS.gene05494 | MS.gene34898 | 0.81383 | 2.10E-51 | -1.69E-46 |
| MS.gene055206 | MS.gene34898 | 0.815581 | 8.59E-52 | -1.69E-46 |
| MS.gene055539 | MS.gene34898 | 0.800577 | 1.35E-48 | -1.69E-46 |
| MS.gene055741 | MS.gene34898 | 0.804345 | 2.26E-49 | -1.69E-46 |
| MS.gene055744 | MS.gene34898 | 0.80475 | 1.86E-49 | -1.69E-46 |
| MS.gene05589 | MS.gene34898 | 0.805835 | 1.10E-49 | -1.69E-46 |
| MS.gene057332 | MS.gene34898 | 0.82548 | 4.58E-54 | -1.69E-46 |
| MS.gene058838 | MS.gene34898 | 0.819063 | 1.41E-52 | -1.69E-46 |
| MS.gene060835 | MS.gene34898 | 0.812221 | 4.73E-51 | -1.69E-46 |
| MS.gene061154 | MS.gene34898 | 0.809923 | 1.49E-50 | -1.69E-46 |
| MS.gene061231 | MS.gene34898 | 0.823096 | 1.67E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34898.t1 | MTR_4g083110 | 79.121 | 91 | 12 | 1 | 1 | 91 | 1 | 84 | 3.00e-39 | 125 |
| MS.gene34898.t1 | MTR_5g077640 | 80.435 | 92 | 15 | 1 | 1 | 92 | 1 | 89 | 7.26e-39 | 124 |
| MS.gene34898.t1 | MTR_4g083130 | 79.348 | 92 | 16 | 1 | 1 | 92 | 1 | 89 | 2.89e-33 | 110 |
| MS.gene34898.t1 | MTR_5g077680 | 96.154 | 52 | 2 | 0 | 1 | 52 | 1 | 52 | 3.29e-32 | 107 |
| MS.gene34898.t1 | MTR_8g079458 | 96.154 | 52 | 2 | 0 | 1 | 52 | 1 | 52 | 3.29e-32 | 107 |
| MS.gene34898.t1 | MTR_4g012020 | 94.231 | 52 | 3 | 0 | 1 | 52 | 1 | 52 | 1.24e-31 | 105 |
| MS.gene34898.t1 | MTR_1437s0010 | 92.308 | 52 | 4 | 0 | 1 | 52 | 1 | 52 | 3.88e-31 | 104 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34898.t1 | AT1G30260 | 59.574 | 47 | 18 | 1 | 1 | 47 | 1 | 46 | 4.36e-15 | 64.7 |
Find 12 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAATTGCTGAAGAGGATA+GGG | 0.483802 | 4.1:-15837074 | MS.gene34898:CDS |
| AAACAATTGCTGAAGAGGAT+AGG | 0.497259 | 4.1:-15837075 | MS.gene34898:CDS |
| GATGGAAACAATTGCTGAAG+AGG | 0.505582 | 4.1:-15837080 | MS.gene34898:CDS |
| GCATAGAACATCAAAGATGA+TGG | 0.512561 | 4.1:-15837098 | MS.gene34898:CDS |
| AACAGTTGCACTTGTAGAAG+AGG | 0.528743 | 4.1:+15836967 | None:intergenic |
| CACAATATGTTTCTGTGATG+AGG | 0.546938 | 4.1:-15837120 | MS.gene34898:CDS |
| GACGGAGCAAGTGATGAAGC+TGG | 0.554923 | 4.1:+15836998 | None:intergenic |
| TGGCAAGTTCTCGCATTGCA+AGG | 0.566271 | 4.1:-15837165 | MS.gene34898:CDS |
| GTAGAAGAGGCGCATGCAGA+CGG | 0.588748 | 4.1:+15836980 | None:intergenic |
| CTCATCACAGAAACATATTG+TGG | 0.621625 | 4.1:+15837121 | None:intergenic |
| TAATACTAGATACTTTCTGA+AGG | 0.630145 | 4.1:-15836945 | MS.gene34898:CDS |
| ATCACAGAAACATATTGTGG+TGG | 0.672845 | 4.1:+15837124 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TAATACTAGATACTTTCTGA+AGG | - | chr4.1:15837131-15837150 | MS.gene34898:CDS | 25.0% |
| !! | GGAATTGTTGATGAACTTTT+AGG | + | chr4.1:15837060-15837079 | None:intergenic | 30.0% |
| AAACAATTGCTGAAGAGGAT+AGG | - | chr4.1:15837001-15837020 | MS.gene34898:CDS | 35.0% | |
| AACAATTGCTGAAGAGGATA+GGG | - | chr4.1:15837002-15837021 | MS.gene34898:CDS | 35.0% | |
| ATCACAGAAACATATTGTGG+TGG | + | chr4.1:15836955-15836974 | None:intergenic | 35.0% | |
| CTCATCACAGAAACATATTG+TGG | + | chr4.1:15836958-15836977 | None:intergenic | 35.0% | |
| GCATAGAACATCAAAGATGA+TGG | - | chr4.1:15836978-15836997 | MS.gene34898:CDS | 35.0% | |
| ! | CACAATATGTTTCTGTGATG+AGG | - | chr4.1:15836956-15836975 | MS.gene34898:CDS | 35.0% |
| AACAGTTGCACTTGTAGAAG+AGG | + | chr4.1:15837112-15837131 | None:intergenic | 40.0% | |
| GATGGAAACAATTGCTGAAG+AGG | - | chr4.1:15836996-15837015 | MS.gene34898:CDS | 40.0% | |
| ! | CATTGCAAGGTTTTTCATGG+AGG | - | chr4.1:15836924-15836943 | MS.gene34898:CDS | 40.0% |
| ! | TCGCATTGCAAGGTTTTTCA+TGG | - | chr4.1:15836921-15836940 | MS.gene34898:CDS | 40.0% |
| TGGCAAGTTCTCGCATTGCA+AGG | - | chr4.1:15836911-15836930 | MS.gene34898:CDS | 50.0% | |
| GACGGAGCAAGTGATGAAGC+TGG | + | chr4.1:15837081-15837100 | None:intergenic | 55.0% | |
| ! | GTAGAAGAGGCGCATGCAGA+CGG | + | chr4.1:15837099-15837118 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 15836910 | 15837188 | 15836910 | ID=MS.gene34898 |
| chr4.1 | mRNA | 15836910 | 15837188 | 15836910 | ID=MS.gene34898.t1;Parent=MS.gene34898 |
| chr4.1 | exon | 15836910 | 15837188 | 15836910 | ID=MS.gene34898.t1.exon1;Parent=MS.gene34898.t1 |
| chr4.1 | CDS | 15836910 | 15837188 | 15836910 | ID=cds.MS.gene34898.t1;Parent=MS.gene34898.t1 |
| Gene Sequence |
| Protein sequence |
