Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051455.t1 | XP_013468157.1 | 93.6 | 110 | 5 | 1 | 1 | 110 | 1 | 108 | 4.50E-51 | 210.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051455.t1 | Q9ZUZ3 | 48.2 | 56 | 28 | 1 | 42 | 96 | 23 | 78 | 5.3e-08 | 58.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051455.t1 | A0A072VJG8 | 93.6 | 110 | 5 | 1 | 1 | 110 | 1 | 108 | 3.3e-51 | 210.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051455 | MS.gene053449 | 0.810196 | 1.30E-50 | -1.69E-46 |
| MS.gene051455 | MS.gene055206 | 0.807472 | 4.97E-50 | -1.69E-46 |
| MS.gene051455 | MS.gene064463 | 0.992392 | 5.12E-193 | -1.69E-46 |
| MS.gene051455 | MS.gene08083 | 0.819669 | 1.03E-52 | -1.69E-46 |
| MS.gene051455 | MS.gene08288 | 0.801603 | 8.33E-49 | -1.69E-46 |
| MS.gene051455 | MS.gene20267 | 0.81397 | 1.95E-51 | -1.69E-46 |
| MS.gene051455 | MS.gene22902 | 0.812134 | 4.94E-51 | -1.69E-46 |
| MS.gene051455 | MS.gene25929 | 0.834822 | 2.40E-56 | -1.69E-46 |
| MS.gene051455 | MS.gene34898 | 0.800401 | 1.47E-48 | -1.69E-46 |
| MS.gene051455 | MS.gene38024 | 0.832867 | 7.40E-56 | -1.69E-46 |
| MS.gene051455 | MS.gene38232 | 0.822412 | 2.40E-53 | -1.69E-46 |
| MS.gene051455 | MS.gene49365 | 0.800703 | 1.27E-48 | -1.69E-46 |
| MS.gene051455 | MS.gene63955 | 0.803655 | 3.14E-49 | -1.69E-46 |
| MS.gene051455 | MS.gene69650 | 0.806753 | 7.06E-50 | -1.69E-46 |
| MS.gene051455 | MS.gene70538 | 0.802663 | 5.04E-49 | -1.69E-46 |
| MS.gene051455 | MS.gene73909 | 0.80812 | 3.62E-50 | -1.69E-46 |
| MS.gene051455 | MS.gene75432 | 0.802025 | 6.82E-49 | -1.69E-46 |
| MS.gene051455 | MS.gene79428 | 0.808975 | 2.38E-50 | -1.69E-46 |
| MS.gene051455 | MS.gene81254 | 0.80635 | 8.59E-50 | -1.69E-46 |
| MS.gene051455 | MS.gene85654 | 0.801378 | 9.26E-49 | -1.69E-46 |
| MS.gene051455 | MS.gene86102 | 0.808002 | 3.84E-50 | -1.69E-46 |
| MS.gene051455 | MS.gene95702 | 0.801965 | 7.02E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051455.t1 | MTR_1g063260 | 93.636 | 110 | 5 | 1 | 1 | 110 | 1 | 108 | 8.35e-72 | 209 |
| MS.gene051455.t1 | MTR_1g063300 | 86.239 | 109 | 11 | 1 | 1 | 109 | 1 | 105 | 2.63e-65 | 192 |
| MS.gene051455.t1 | MTR_1g063280 | 87.037 | 108 | 13 | 1 | 1 | 108 | 1 | 107 | 1.19e-64 | 191 |
| MS.gene051455.t1 | MTR_1g063290 | 85.455 | 110 | 12 | 1 | 1 | 110 | 1 | 106 | 8.34e-64 | 189 |
| MS.gene051455.t1 | MTR_1g063330 | 55.046 | 109 | 38 | 1 | 1 | 109 | 1 | 98 | 2.50e-36 | 119 |
| MS.gene051455.t1 | MTR_1g063340 | 52.294 | 109 | 41 | 1 | 1 | 109 | 1 | 98 | 8.90e-35 | 115 |
| MS.gene051455.t1 | MTR_1g063310 | 55.140 | 107 | 31 | 1 | 1 | 107 | 1 | 90 | 8.15e-34 | 113 |
| MS.gene051455.t1 | MTR_7g101890 | 47.297 | 74 | 39 | 0 | 37 | 110 | 46 | 119 | 8.17e-23 | 85.9 |
| MS.gene051455.t1 | MTR_4g057810 | 50.725 | 69 | 34 | 0 | 40 | 108 | 35 | 103 | 5.34e-19 | 76.3 |
| MS.gene051455.t1 | MTR_2g095430 | 41.558 | 77 | 43 | 1 | 34 | 108 | 36 | 112 | 2.83e-17 | 72.0 |
| MS.gene051455.t1 | MTR_7g101920 | 44.776 | 67 | 37 | 0 | 43 | 109 | 35 | 101 | 4.17e-16 | 68.6 |
| MS.gene051455.t1 | MTR_3g092220 | 32.292 | 96 | 61 | 2 | 19 | 110 | 8 | 103 | 7.75e-15 | 65.5 |
| MS.gene051455.t1 | MTR_4g057800 | 46.377 | 69 | 35 | 1 | 39 | 105 | 34 | 102 | 2.81e-14 | 64.3 |
| MS.gene051455.t1 | MTR_8g026730 | 43.548 | 62 | 35 | 0 | 43 | 104 | 27 | 88 | 2.25e-13 | 61.2 |
| MS.gene051455.t1 | MTR_1g009150 | 44.444 | 72 | 38 | 1 | 34 | 105 | 62 | 131 | 1.91e-12 | 60.5 |
| MS.gene051455.t1 | MTR_7g118010 | 41.935 | 62 | 36 | 0 | 43 | 104 | 23 | 84 | 2.56e-12 | 58.5 |
| MS.gene051455.t1 | MTR_4g005320 | 45.455 | 66 | 34 | 1 | 40 | 105 | 67 | 130 | 6.81e-12 | 58.9 |
| MS.gene051455.t1 | MTR_1g061570 | 38.889 | 72 | 42 | 1 | 34 | 105 | 10 | 79 | 2.39e-11 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051455.t1 | AT3G09870 | 42.308 | 78 | 44 | 1 | 34 | 110 | 36 | 113 | 2.27e-17 | 72.0 |
| MS.gene051455.t1 | AT2G28085 | 47.222 | 72 | 36 | 1 | 39 | 108 | 51 | 122 | 5.37e-15 | 65.9 |
| MS.gene051455.t1 | AT4G00880 | 33.019 | 106 | 55 | 3 | 8 | 110 | 6 | 98 | 1.77e-12 | 59.7 |
| MS.gene051455.t1 | AT2G46690 | 39.726 | 73 | 40 | 2 | 42 | 110 | 23 | 95 | 7.39e-12 | 57.8 |
| MS.gene051455.t1 | AT3G61900 | 38.356 | 73 | 44 | 1 | 25 | 96 | 12 | 84 | 7.79e-12 | 58.2 |
| MS.gene051455.t1 | AT3G43120 | 42.254 | 71 | 39 | 1 | 27 | 97 | 63 | 131 | 1.10e-11 | 58.2 |
Find 19 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATGCTTGATTCATTTGT+TGG | 0.341379 | 1.1:+47970118 | None:intergenic |
| GATGATGCTTGGGAGGAGTA+TGG | 0.392995 | 1.1:+47970352 | MS.gene051455:CDS |
| CTTTGCTGTTCTTGCAAAGA+AGG | 0.394733 | 1.1:+47970261 | MS.gene051455:CDS |
| TGGGATTGCTTGATGATGCT+TGG | 0.395014 | 1.1:+47970341 | MS.gene051455:CDS |
| GGGATTGCTTGATGATGCTT+GGG | 0.409014 | 1.1:+47970342 | MS.gene051455:CDS |
| GAAACTAGAAGGTTTATTGT+TGG | 0.428359 | 1.1:+47970289 | MS.gene051455:CDS |
| AAATGCTTGATTCATTTGTT+GGG | 0.431852 | 1.1:+47970119 | None:intergenic |
| CTCACTTGTGGTTGTGATTG+TGG | 0.437138 | 1.1:-47970212 | None:intergenic |
| GGAGTATGGTTTCAGACAAA+AGG | 0.443046 | 1.1:+47970366 | MS.gene051455:CDS |
| AGAAGGGTGTAGAAACTAGA+AGG | 0.457872 | 1.1:+47970278 | MS.gene051455:CDS |
| GCTTGATTCATTTGTTGGGA+AGG | 0.508242 | 1.1:+47970123 | MS.gene051455:CDS |
| GAACTCTCGTTGTTCCGTGC+CGG | 0.527691 | 1.1:+47970389 | MS.gene051455:CDS |
| TACAAAACATTCTAGATAGT+CGG | 0.531235 | 1.1:+47970422 | MS.gene051455:CDS |
| TTTGCTGTTCTTGCAAAGAA+GGG | 0.553621 | 1.1:+47970262 | MS.gene051455:CDS |
| GAGTATGGTTTCAGACAAAA+GGG | 0.556094 | 1.1:+47970367 | MS.gene051455:CDS |
| ATCATCAGCAAGCTCACTTG+TGG | 0.599516 | 1.1:-47970224 | None:intergenic |
| ATTGCTTGATGATGCTTGGG+AGG | 0.606537 | 1.1:+47970345 | MS.gene051455:CDS |
| CTTGCTGATGATGTTATGAA+AGG | 0.606752 | 1.1:+47970235 | MS.gene051455:CDS |
| TGTAAGTCGATCGGCCGGCA+CGG | 0.614103 | 1.1:-47970403 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TACAAAACATTCTAGATAGT+CGG | + | chr1.1:47970422-47970441 | MS.gene051455:CDS | 25.0% |
| GAAACTAGAAGGTTTATTGT+TGG | + | chr1.1:47970289-47970308 | MS.gene051455:CDS | 30.0% | |
| ! | AGAATGTTTTGTAAGTCGAT+CGG | - | chr1.1:47970415-47970434 | None:intergenic | 30.0% |
| !!! | TAACTCAGTGCATGTTTTTT+TGG | - | chr1.1:47970175-47970194 | None:intergenic | 30.0% |
| !!! | TTAACTGATCCTGCTTTTTT+GGG | + | chr1.1:47970322-47970341 | MS.gene051455:CDS | 30.0% |
| AAAAACATGCACTGAGTTAC+AGG | + | chr1.1:47970176-47970195 | MS.gene051455:CDS | 35.0% | |
| GAGTATGGTTTCAGACAAAA+GGG | + | chr1.1:47970367-47970386 | MS.gene051455:CDS | 35.0% | |
| ! | CTTGCTGATGATGTTATGAA+AGG | + | chr1.1:47970235-47970254 | MS.gene051455:CDS | 35.0% |
| !! | TTTGCTGTTCTTGCAAAGAA+GGG | + | chr1.1:47970262-47970281 | MS.gene051455:CDS | 35.0% |
| !!! | CTTAACTGATCCTGCTTTTT+TGG | + | chr1.1:47970321-47970340 | MS.gene051455:CDS | 35.0% |
| GGAGTATGGTTTCAGACAAA+AGG | + | chr1.1:47970366-47970385 | MS.gene051455:CDS | 40.0% | |
| TCAAGCAATCCCAAAAAAGC+AGG | - | chr1.1:47970334-47970353 | None:intergenic | 40.0% | |
| ! | AGAAGGGTGTAGAAACTAGA+AGG | + | chr1.1:47970278-47970297 | MS.gene051455:CDS | 40.0% |
| ! | GCTTGATTCATTTGTTGGGA+AGG | + | chr1.1:47970123-47970142 | MS.gene051455:CDS | 40.0% |
| !! | CTTTGCTGTTCTTGCAAAGA+AGG | + | chr1.1:47970261-47970280 | MS.gene051455:CDS | 40.0% |
| ATCATCAGCAAGCTCACTTG+TGG | - | chr1.1:47970227-47970246 | None:intergenic | 45.0% | |
| CTCACTTGTGGTTGTGATTG+TGG | - | chr1.1:47970215-47970234 | None:intergenic | 45.0% | |
| ! | TGTTTTGTAAGTCGATCGGC+CGG | - | chr1.1:47970411-47970430 | None:intergenic | 45.0% |
| !! | ATTGCTTGATGATGCTTGGG+AGG | + | chr1.1:47970345-47970364 | MS.gene051455:CDS | 45.0% |
| !! | GGGATTGCTTGATGATGCTT+GGG | + | chr1.1:47970342-47970361 | MS.gene051455:CDS | 45.0% |
| !! | TGGGATTGCTTGATGATGCT+TGG | + | chr1.1:47970341-47970360 | MS.gene051455:CDS | 45.0% |
| GATGATGCTTGGGAGGAGTA+TGG | + | chr1.1:47970352-47970371 | MS.gene051455:CDS | 50.0% | |
| GAACTCTCGTTGTTCCGTGC+CGG | + | chr1.1:47970389-47970408 | MS.gene051455:CDS | 55.0% | |
| TGTAAGTCGATCGGCCGGCA+CGG | - | chr1.1:47970406-47970425 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 47970121 | 47970453 | 47970121 | ID=MS.gene051455 |
| chr1.1 | mRNA | 47970121 | 47970453 | 47970121 | ID=MS.gene051455.t1;Parent=MS.gene051455 |
| chr1.1 | exon | 47970121 | 47970453 | 47970121 | ID=MS.gene051455.t1.exon1;Parent=MS.gene051455.t1 |
| chr1.1 | CDS | 47970121 | 47970453 | 47970121 | ID=cds.MS.gene051455.t1;Parent=MS.gene051455.t1 |
| Gene Sequence |
| Protein sequence |
