Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36867.t1 | XP_013444914.1 | 89.7 | 184 | 18 | 1 | 1 | 183 | 1 | 184 | 4.50E-88 | 334 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36867.t1 | A0A072TN78 | 89.7 | 184 | 18 | 1 | 1 | 183 | 1 | 184 | 3.3e-88 | 334.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050945 | MS.gene36867 | 0.828165 | 1.05E-54 | -1.69E-46 |
| MS.gene051960 | MS.gene36867 | 0.81053 | 1.10E-50 | -1.69E-46 |
| MS.gene053879 | MS.gene36867 | 0.809352 | 1.97E-50 | -1.69E-46 |
| MS.gene054413 | MS.gene36867 | 0.804767 | 1.84E-49 | -1.69E-46 |
| MS.gene055150 | MS.gene36867 | 0.846172 | 2.57E-59 | -1.69E-46 |
| MS.gene055886 | MS.gene36867 | 0.828944 | 6.78E-55 | -1.69E-46 |
| MS.gene057174 | MS.gene36867 | 0.812815 | 3.51E-51 | -1.69E-46 |
| MS.gene05952 | MS.gene36867 | 0.815426 | 9.30E-52 | -1.69E-46 |
| MS.gene05985 | MS.gene36867 | -0.811165 | 8.03E-51 | -1.69E-46 |
| MS.gene06023 | MS.gene36867 | 0.80536 | 1.39E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36867.t1 | MTR_8g432440 | 89.674 | 184 | 18 | 1 | 1 | 183 | 1 | 184 | 4.35e-121 | 340 |
| MS.gene36867.t1 | MTR_8g432420 | 89.674 | 184 | 18 | 1 | 1 | 183 | 1 | 184 | 4.35e-121 | 340 |
| MS.gene36867.t1 | MTR_8g432460 | 88.525 | 183 | 21 | 0 | 1 | 183 | 1 | 183 | 5.12e-120 | 337 |
| MS.gene36867.t1 | MTR_8g432470 | 85.870 | 184 | 25 | 1 | 1 | 183 | 1 | 184 | 7.33e-115 | 324 |
| MS.gene36867.t1 | MTR_8g432490 | 83.607 | 183 | 30 | 0 | 1 | 183 | 1 | 183 | 1.92e-112 | 318 |
| MS.gene36867.t1 | MTR_3g064780 | 80.556 | 180 | 35 | 0 | 1 | 180 | 1 | 180 | 2.22e-106 | 305 |
| MS.gene36867.t1 | MTR_8g432450 | 84.746 | 177 | 25 | 2 | 1 | 175 | 1 | 177 | 1.26e-105 | 300 |
| MS.gene36867.t1 | MTR_8g432480 | 54.545 | 176 | 58 | 6 | 5 | 174 | 7 | 166 | 3.87e-50 | 160 |
| MS.gene36867.t1 | MTR_7g076000 | 34.637 | 179 | 89 | 6 | 5 | 176 | 2 | 159 | 5.78e-24 | 92.8 |
| MS.gene36867.t1 | MTR_8g432400 | 34.426 | 183 | 103 | 6 | 5 | 179 | 2 | 175 | 5.25e-22 | 88.2 |
| MS.gene36867.t1 | MTR_8g432370 | 34.783 | 184 | 100 | 7 | 5 | 179 | 2 | 174 | 1.69e-21 | 86.7 |
| MS.gene36867.t1 | MTR_8g432350 | 34.615 | 182 | 99 | 7 | 5 | 177 | 2 | 172 | 1.89e-20 | 84.0 |
| MS.gene36867.t1 | MTR_8g432390 | 34.595 | 185 | 100 | 8 | 5 | 179 | 2 | 175 | 1.95e-19 | 81.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 49 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACTCTAATGGTGTTGTTAT+TGG | 0.174838 | 8.4:-64130758 | MS.gene36867:CDS |
| TGAGTTATAAATTGAGGAAT+TGG | 0.246315 | 8.4:-64130925 | MS.gene36867:CDS |
| AACAACACCATTAGAGTTCT+TGG | 0.251508 | 8.4:+64130763 | None:intergenic |
| AATTATGGTGGTCGGTTAAC+TGG | 0.256041 | 8.4:-64130800 | MS.gene36867:CDS |
| ATAAATATTTAAATAATGTT+TGG | 0.290889 | 8.4:+64130713 | None:intergenic |
| TCAAAGGTATTCGACCTATT+GGG | 0.300168 | 8.4:-64131012 | MS.gene36867:CDS |
| TTCAAAGGTATTCGACCTAT+TGG | 0.303554 | 8.4:-64131013 | MS.gene36867:CDS |
| TTAGAAATTACGGTTATATC+TGG | 0.325832 | 8.4:-64131229 | MS.gene36867:CDS |
| TTCACAAACATTAGAAATTA+CGG | 0.327531 | 8.4:-64131239 | MS.gene36867:CDS |
| AATTGGGAAGGGAGGCAAAA+TGG | 0.332260 | 8.4:-64130908 | MS.gene36867:CDS |
| TAGTGTGAGGGTGGTTGTTC+CGG | 0.338986 | 8.4:-64130873 | MS.gene36867:CDS |
| TAAATATTTAAATAATGTTT+GGG | 0.348234 | 8.4:+64130714 | None:intergenic |
| GTGGCGGATGGAAAGAATTA+TGG | 0.368827 | 8.4:-64130815 | MS.gene36867:CDS |
| TTGTTATTGGTATTCCGTTT+TGG | 0.371495 | 8.4:-64130745 | MS.gene36867:CDS |
| GCAATTCTTGCCACCCCAAT+AGG | 0.391470 | 8.4:+64130998 | None:intergenic |
| GGTGGCAAGAATTGCTCTTT+CGG | 0.416259 | 8.4:-64130990 | MS.gene36867:CDS |
| CACCGTTTGCTTCTCCGCCT+CGG | 0.445504 | 8.4:+64130835 | None:intergenic |
| GAGTTATAAATTGAGGAATT+GGG | 0.456017 | 8.4:-64130924 | MS.gene36867:CDS |
| TATTCTCAATGGCGCGGTAT+CGG | 0.469676 | 8.4:-64130966 | MS.gene36867:CDS |
| GCTCTTTCGGATATTCTCAA+TGG | 0.472815 | 8.4:-64130977 | MS.gene36867:CDS |
| TGAAGGAAAATGATAATTGT+GGG | 0.480545 | 8.4:-64131129 | MS.gene36867:CDS |
| GGCGGAGAAGCAAACGGTGG+CGG | 0.482611 | 8.4:-64130831 | MS.gene36867:CDS |
| GTGAAGGAAAATGATAATTG+TGG | 0.484213 | 8.4:-64131130 | MS.gene36867:CDS |
| TTTGGGTAATTCCACCAAAA+CGG | 0.496948 | 8.4:+64130731 | None:intergenic |
| TGCAAGGTCAATCACGTTTG+AGG | 0.523501 | 8.4:-64131050 | MS.gene36867:CDS |
| CAAAGGTATTCGACCTATTG+GGG | 0.532466 | 8.4:-64131011 | MS.gene36867:CDS |
| CGGTTAACTGGAATGGATGT+TGG | 0.534432 | 8.4:-64130788 | MS.gene36867:CDS |
| GAGAAGCAAACGGTGGCGGA+TGG | 0.538736 | 8.4:-64130827 | MS.gene36867:CDS |
| TGGTGGTCGGTTAACTGGAA+TGG | 0.553085 | 8.4:-64130795 | MS.gene36867:CDS |
| GTTGGTGCCAAGAACTCTAA+TGG | 0.557347 | 8.4:-64130770 | MS.gene36867:CDS |
| TCGGTATCCGATCTTTGCTC+CGG | 0.559233 | 8.4:+64130854 | None:intergenic |
| AATTGAGGAATTGGGAAGGG+AGG | 0.560763 | 8.4:-64130916 | MS.gene36867:CDS |
| GGTTGTTCCGGAGCAAAGAT+CGG | 0.564026 | 8.4:-64130861 | MS.gene36867:CDS |
| TGAACATGCCATTGCATGCA+AGG | 0.566598 | 8.4:-64131066 | MS.gene36867:CDS |
| TATAAATTGAGGAATTGGGA+AGG | 0.569289 | 8.4:-64130920 | MS.gene36867:CDS |
| GATGCATACGTTGTTGTTCG+AGG | 0.569601 | 8.4:-64131184 | MS.gene36867:CDS |
| ATAAATTGAGGAATTGGGAA+GGG | 0.583144 | 8.4:-64130919 | MS.gene36867:CDS |
| TACCGAGGCGGAGAAGCAAA+CGG | 0.584351 | 8.4:-64130837 | MS.gene36867:CDS |
| GTGCAATGCAAGAAGTTCAA+AGG | 0.594547 | 8.4:-64131028 | MS.gene36867:CDS |
| TTCGGATATTCTCAATGGCG+CGG | 0.599946 | 8.4:-64130972 | MS.gene36867:CDS |
| GTGATTGACCTTGCATGCAA+TGG | 0.618595 | 8.4:+64131058 | None:intergenic |
| CGAAAATATTCATGTAACAG+AGG | 0.621033 | 8.4:-64131206 | MS.gene36867:CDS |
| ATGGAAAGAATTATGGTGGT+CGG | 0.623818 | 8.4:-64130808 | MS.gene36867:CDS |
| GCGGATGGAAAGAATTATGG+TGG | 0.668878 | 8.4:-64130812 | MS.gene36867:CDS |
| AGGTATTCGACCTATTGGGG+TGG | 0.674947 | 8.4:-64131008 | MS.gene36867:CDS |
| GCAAAGATCGGATACCGAGG+CGG | 0.694888 | 8.4:-64130849 | MS.gene36867:CDS |
| TGTTTGTGAAGTCACAACCA+TGG | 0.699218 | 8.4:+64131252 | None:intergenic |
| GGAGCAAAGATCGGATACCG+AGG | 0.705796 | 8.4:-64130852 | MS.gene36867:CDS |
| CGAGGCGGAGAAGCAAACGG+TGG | 0.746726 | 8.4:-64130834 | MS.gene36867:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATAGTTACACAACAAAAA+CGG | - | chr8.4:64130819-64130838 | MS.gene36867:CDS | 20.0% |
| !! | TTCACAAACATTAGAAATTA+CGG | - | chr8.4:64130732-64130751 | MS.gene36867:CDS | 20.0% |
| !!! | GAATTTTGAGTTATAAATTG+AGG | - | chr8.4:64131040-64131059 | MS.gene36867:CDS | 20.0% |
| ! | GAGTTATAAATTGAGGAATT+GGG | - | chr8.4:64131047-64131066 | MS.gene36867:CDS | 25.0% |
| ! | TGAAGGAAAATGATAATTGT+GGG | - | chr8.4:64130842-64130861 | MS.gene36867:CDS | 25.0% |
| ! | TGAGTTATAAATTGAGGAAT+TGG | - | chr8.4:64131046-64131065 | MS.gene36867:CDS | 25.0% |
| ! | TTAGAAATTACGGTTATATC+TGG | - | chr8.4:64130742-64130761 | MS.gene36867:CDS | 25.0% |
| !!! | AAAACTCGAGTTTTATTTCA+TGG | - | chr8.4:64130866-64130885 | MS.gene36867:CDS | 25.0% |
| !!! | AGTTATTCATTTTAGTGTGA+GGG | - | chr8.4:64131086-64131105 | MS.gene36867:CDS | 25.0% |
| ATAAATTGAGGAATTGGGAA+GGG | - | chr8.4:64131052-64131071 | MS.gene36867:CDS | 30.0% | |
| CGAAAATATTCATGTAACAG+AGG | - | chr8.4:64130765-64130784 | MS.gene36867:CDS | 30.0% | |
| GTGAAGGAAAATGATAATTG+TGG | - | chr8.4:64130841-64130860 | MS.gene36867:CDS | 30.0% | |
| TATAAATTGAGGAATTGGGA+AGG | - | chr8.4:64131051-64131070 | MS.gene36867:CDS | 30.0% | |
| !! | AACTCTAATGGTGTTGTTAT+TGG | - | chr8.4:64131213-64131232 | MS.gene36867:CDS | 30.0% |
| !! | GAGTTATTCATTTTAGTGTG+AGG | - | chr8.4:64131085-64131104 | MS.gene36867:CDS | 30.0% |
| !! | TTGTTATTGGTATTCCGTTT+TGG | - | chr8.4:64131226-64131245 | MS.gene36867:CDS | 30.0% |
| AACAACACCATTAGAGTTCT+TGG | + | chr8.4:64131211-64131230 | None:intergenic | 35.0% | |
| ATGGAAAGAATTATGGTGGT+CGG | - | chr8.4:64131163-64131182 | MS.gene36867:CDS | 35.0% | |
| TTACACAACAAAAACGGTGA+AGG | - | chr8.4:64130825-64130844 | MS.gene36867:CDS | 35.0% | |
| TTTGGGTAATTCCACCAAAA+CGG | + | chr8.4:64131243-64131262 | None:intergenic | 35.0% | |
| ! | TTATTGGTATTCCGTTTTGG+TGG | - | chr8.4:64131229-64131248 | MS.gene36867:CDS | 35.0% |
| !! | TATTCATTTTAGTGTGAGGG+TGG | - | chr8.4:64131089-64131108 | MS.gene36867:CDS | 35.0% |
| !! | TCAAAGGTATTCGACCTATT+GGG | - | chr8.4:64130959-64130978 | MS.gene36867:CDS | 35.0% |
| !! | TTCAAAGGTATTCGACCTAT+TGG | - | chr8.4:64130958-64130977 | MS.gene36867:CDS | 35.0% |
| AATTATGGTGGTCGGTTAAC+TGG | - | chr8.4:64131171-64131190 | MS.gene36867:CDS | 40.0% | |
| GCTCTTTCGGATATTCTCAA+TGG | - | chr8.4:64130994-64131013 | MS.gene36867:CDS | 40.0% | |
| GTGCAATGCAAGAAGTTCAA+AGG | - | chr8.4:64130943-64130962 | MS.gene36867:CDS | 40.0% | |
| !! | CAAAGGTATTCGACCTATTG+GGG | - | chr8.4:64130960-64130979 | MS.gene36867:CDS | 40.0% |
| AATTGAGGAATTGGGAAGGG+AGG | - | chr8.4:64131055-64131074 | MS.gene36867:CDS | 45.0% | |
| AATTGGGAAGGGAGGCAAAA+TGG | - | chr8.4:64131063-64131082 | MS.gene36867:CDS | 45.0% | |
| CGGTTAACTGGAATGGATGT+TGG | - | chr8.4:64131183-64131202 | MS.gene36867:CDS | 45.0% | |
| GCGGATGGAAAGAATTATGG+TGG | - | chr8.4:64131159-64131178 | MS.gene36867:CDS | 45.0% | |
| GGTGGCAAGAATTGCTCTTT+CGG | - | chr8.4:64130981-64131000 | MS.gene36867:CDS | 45.0% | |
| GTGATTGACCTTGCATGCAA+TGG | + | chr8.4:64130916-64130935 | None:intergenic | 45.0% | |
| TATTCTCAATGGCGCGGTAT+CGG | - | chr8.4:64131005-64131024 | MS.gene36867:CDS | 45.0% | |
| TGAACATGCCATTGCATGCA+AGG | - | chr8.4:64130905-64130924 | MS.gene36867:CDS | 45.0% | |
| TGCAAGGTCAATCACGTTTG+AGG | - | chr8.4:64130921-64130940 | MS.gene36867:CDS | 45.0% | |
| TTCGGATATTCTCAATGGCG+CGG | - | chr8.4:64130999-64131018 | MS.gene36867:CDS | 45.0% | |
| ! | GATGCATACGTTGTTGTTCG+AGG | - | chr8.4:64130787-64130806 | MS.gene36867:CDS | 45.0% |
| !! | GTGGCGGATGGAAAGAATTA+TGG | - | chr8.4:64131156-64131175 | MS.gene36867:CDS | 45.0% |
| !! | GTTGGTGCCAAGAACTCTAA+TGG | - | chr8.4:64131201-64131220 | MS.gene36867:CDS | 45.0% |
| AGGTATTCGACCTATTGGGG+TGG | - | chr8.4:64130963-64130982 | MS.gene36867:CDS | 50.0% | |
| GCAATTCTTGCCACCCCAAT+AGG | + | chr8.4:64130976-64130995 | None:intergenic | 50.0% | |
| GGTTGTTCCGGAGCAAAGAT+CGG | - | chr8.4:64131110-64131129 | MS.gene36867:CDS | 50.0% | |
| TAGTGTGAGGGTGGTTGTTC+CGG | - | chr8.4:64131098-64131117 | MS.gene36867:CDS | 50.0% | |
| TCGGTATCCGATCTTTGCTC+CGG | + | chr8.4:64131120-64131139 | None:intergenic | 50.0% | |
| TGGTGGTCGGTTAACTGGAA+TGG | - | chr8.4:64131176-64131195 | MS.gene36867:CDS | 50.0% | |
| GCAAAGATCGGATACCGAGG+CGG | - | chr8.4:64131122-64131141 | MS.gene36867:CDS | 55.0% | |
| GGAGCAAAGATCGGATACCG+AGG | - | chr8.4:64131119-64131138 | MS.gene36867:CDS | 55.0% | |
| TACCGAGGCGGAGAAGCAAA+CGG | - | chr8.4:64131134-64131153 | MS.gene36867:CDS | 55.0% | |
| CACCGTTTGCTTCTCCGCCT+CGG | + | chr8.4:64131139-64131158 | None:intergenic | 60.0% | |
| !! | GAGAAGCAAACGGTGGCGGA+TGG | - | chr8.4:64131144-64131163 | MS.gene36867:CDS | 60.0% |
| CGAGGCGGAGAAGCAAACGG+TGG | - | chr8.4:64131137-64131156 | MS.gene36867:CDS | 65.0% | |
| GGCGGAGAAGCAAACGGTGG+CGG | - | chr8.4:64131140-64131159 | MS.gene36867:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 64130721 | 64131272 | 64130721 | ID=MS.gene36867 |
| chr8.4 | mRNA | 64130721 | 64131272 | 64130721 | ID=MS.gene36867.t1;Parent=MS.gene36867 |
| chr8.4 | exon | 64130721 | 64131272 | 64130721 | ID=MS.gene36867.t1.exon1;Parent=MS.gene36867.t1 |
| chr8.4 | CDS | 64130721 | 64131272 | 64130721 | ID=cds.MS.gene36867.t1;Parent=MS.gene36867.t1 |
| Gene Sequence |
| Protein sequence |
