Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37742.t1 | XP_003620187.1 | 96.2 | 211 | 8 | 0 | 1 | 211 | 1 | 211 | 2.00E-116 | 428.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37742.t1 | P13087 | 40.8 | 211 | 104 | 6 | 11 | 211 | 17 | 216 | 4.4e-35 | 149.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37742.t1 | G7KKQ9 | 96.2 | 211 | 8 | 0 | 1 | 211 | 1 | 211 | 1.5e-116 | 428.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050426 | MS.gene37742 | 0.801577 | 8.43E-49 | -1.69E-46 |
| MS.gene050971 | MS.gene37742 | -0.800809 | 1.21E-48 | -1.69E-46 |
| MS.gene052011 | MS.gene37742 | 0.814576 | 1.44E-51 | -1.69E-46 |
| MS.gene052223 | MS.gene37742 | 0.814156 | 1.78E-51 | -1.69E-46 |
| MS.gene052253 | MS.gene37742 | -0.801819 | 7.52E-49 | -1.69E-46 |
| MS.gene05233 | MS.gene37742 | 0.960581 | 1.02E-118 | -1.69E-46 |
| MS.gene053406 | MS.gene37742 | 0.809475 | 1.86E-50 | -1.69E-46 |
| MS.gene055601 | MS.gene37742 | 0.842302 | 2.81E-58 | -1.69E-46 |
| MS.gene056330 | MS.gene37742 | 0.815376 | 9.54E-52 | -1.69E-46 |
| MS.gene056690 | MS.gene37742 | 0.803431 | 3.50E-49 | -1.69E-46 |
| MS.gene056899 | MS.gene37742 | 0.845752 | 3.34E-59 | -1.69E-46 |
| MS.gene058218 | MS.gene37742 | 0.814834 | 1.26E-51 | -1.69E-46 |
| MS.gene058928 | MS.gene37742 | -0.808585 | 2.88E-50 | -1.69E-46 |
| MS.gene059420 | MS.gene37742 | -0.813652 | 2.30E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37742.t1 | MTR_6g078280 | 95.755 | 212 | 9 | 0 | 1 | 212 | 1 | 212 | 6.77e-145 | 402 |
| MS.gene37742.t1 | MTR_6g078260 | 68.372 | 215 | 47 | 6 | 1 | 211 | 1 | 198 | 1.74e-95 | 277 |
| MS.gene37742.t1 | MTR_6g078140 | 60.697 | 201 | 71 | 5 | 15 | 211 | 16 | 212 | 2.47e-75 | 226 |
| MS.gene37742.t1 | MTR_6g078120 | 60.199 | 201 | 72 | 5 | 15 | 211 | 16 | 212 | 1.42e-74 | 224 |
| MS.gene37742.t1 | MTR_6g078290 | 53.488 | 215 | 90 | 6 | 1 | 210 | 1 | 210 | 2.51e-69 | 211 |
| MS.gene37742.t1 | MTR_6g078250 | 53.023 | 215 | 87 | 5 | 1 | 212 | 1 | 204 | 9.86e-66 | 201 |
| MS.gene37742.t1 | MTR_7g037410 | 44.444 | 189 | 90 | 5 | 26 | 211 | 25 | 201 | 2.06e-50 | 162 |
| MS.gene37742.t1 | MTR_6g078100 | 38.140 | 215 | 118 | 8 | 1 | 211 | 1 | 204 | 7.87e-35 | 122 |
| MS.gene37742.t1 | MTR_6g078070 | 36.530 | 219 | 114 | 9 | 1 | 211 | 1 | 202 | 1.04e-31 | 114 |
| MS.gene37742.t1 | MTR_6g065460 | 29.577 | 213 | 134 | 7 | 1 | 209 | 1 | 201 | 6.14e-15 | 70.9 |
| MS.gene37742.t1 | MTR_6g009563 | 30.049 | 203 | 119 | 7 | 6 | 203 | 9 | 193 | 3.40e-13 | 66.2 |
| MS.gene37742.t1 | MTR_6g009650 | 30.244 | 205 | 118 | 7 | 4 | 203 | 8 | 192 | 1.89e-12 | 64.3 |
| MS.gene37742.t1 | MTR_3g106620 | 30.222 | 225 | 113 | 13 | 6 | 212 | 1 | 199 | 6.03e-12 | 62.8 |
| MS.gene37742.t1 | MTR_8g461050 | 28.774 | 212 | 126 | 9 | 9 | 211 | 6 | 201 | 6.72e-12 | 62.4 |
| MS.gene37742.t1 | MTR_0084s0030 | 28.505 | 214 | 124 | 11 | 9 | 211 | 6 | 201 | 2.27e-11 | 60.8 |
| MS.gene37742.t1 | MTR_3g106585 | 28.972 | 214 | 119 | 12 | 6 | 212 | 10 | 197 | 5.95e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37742.t1 | AT1G17860 | 37.037 | 216 | 109 | 8 | 1 | 211 | 1 | 194 | 1.71e-38 | 132 |
| MS.gene37742.t1 | AT1G73325 | 32.889 | 225 | 114 | 11 | 1 | 211 | 1 | 202 | 7.63e-22 | 89.7 |
| MS.gene37742.t1 | AT1G73260 | 30.332 | 211 | 124 | 9 | 8 | 212 | 12 | 205 | 9.19e-20 | 84.0 |
| MS.gene37742.t1 | AT1G73330 | 30.769 | 221 | 115 | 13 | 1 | 212 | 1 | 192 | 2.26e-15 | 72.0 |
Find 55 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCTGAAACTTAACTTTAA+AGG | 0.154767 | 6.3:-72167274 | None:intergenic |
| CATCTTCATACTTCTCAATT+TGG | 0.163418 | 6.3:-72167137 | None:intergenic |
| AATGTTTCTTCTTTAGAAAA+AGG | 0.285974 | 6.3:-72167057 | None:intergenic |
| TTCTGAAACTTAACTTTAAA+GGG | 0.298093 | 6.3:-72167273 | None:intergenic |
| ACAAAGCCACATGTTGTAAA+TGG | 0.312447 | 6.3:-72166814 | None:intergenic |
| AGCCGGAATGATGTAGTAAT+TGG | 0.367058 | 6.3:-72166788 | None:intergenic |
| AACCATGTATGCAAAGATAT+TGG | 0.371437 | 6.3:+72167201 | MS.gene37742:CDS |
| AACGCCTTTCTTGTTGTTAA+CGG | 0.386453 | 6.3:-72166944 | None:intergenic |
| TTGTTAGTTGTTTCAATAGT+GGG | 0.387109 | 6.3:+72166833 | MS.gene37742:CDS |
| GTGACTACTAATGGTGTATT+GGG | 0.404413 | 6.3:+72167081 | MS.gene37742:CDS |
| ACGCCTTTCTTGTTGTTAAC+GGG | 0.404872 | 6.3:-72166943 | None:intergenic |
| TTCCAATATCTTTGCATACA+TGG | 0.418385 | 6.3:-72167203 | None:intergenic |
| GAAACATTTGTGACTACTAA+TGG | 0.419288 | 6.3:+72167072 | MS.gene37742:CDS |
| TTTGTTAGTTGTTTCAATAG+TGG | 0.428561 | 6.3:+72166832 | MS.gene37742:CDS |
| TGCATACATGGTTGCAAGTA+GGG | 0.429484 | 6.3:-72167191 | None:intergenic |
| TGTGACTACTAATGGTGTAT+TGG | 0.442930 | 6.3:+72167080 | MS.gene37742:CDS |
| TACCAATTACTACATCATTC+CGG | 0.457107 | 6.3:+72166786 | MS.gene37742:CDS |
| TAAGACTAGGGAAATGCGTT+TGG | 0.471251 | 6.3:+72167236 | MS.gene37742:CDS |
| GTTCAAATACAATCCACAAT+TGG | 0.476445 | 6.3:+72167112 | MS.gene37742:CDS |
| TCTCAATTTGGAACCAATTG+TGG | 0.486271 | 6.3:-72167125 | None:intergenic |
| TTGTCCTCACAACCACTACT+TGG | 0.492527 | 6.3:+72166703 | MS.gene37742:CDS |
| CATGTTGTAAATGGCGTAGC+CGG | 0.496350 | 6.3:-72166805 | None:intergenic |
| CGATGTGTCAATGACTTGAT+CGG | 0.502159 | 6.3:-72166743 | None:intergenic |
| GAACTTTATGTTGAGATCAG+TGG | 0.505826 | 6.3:-72166977 | None:intergenic |
| TTGCAAGTAGGGCACACATT+AGG | 0.510934 | 6.3:-72167180 | None:intergenic |
| GAATTTATGTGTATAAGACT+AGG | 0.515322 | 6.3:+72167223 | MS.gene37742:CDS |
| TTGCATACATGGTTGCAAGT+AGG | 0.520717 | 6.3:-72167192 | None:intergenic |
| TGTTAGTTGTTTCAATAGTG+GGG | 0.526839 | 6.3:+72166834 | MS.gene37742:CDS |
| GTAGTTGTGAAACATAATCA+AGG | 0.526996 | 6.3:+72166901 | MS.gene37742:CDS |
| AATTTATGTGTATAAGACTA+GGG | 0.531831 | 6.3:+72167224 | MS.gene37742:CDS |
| AGGATCAATCTTCCACACTA+AGG | 0.534325 | 6.3:-72167037 | None:intergenic |
| GTTAGTTGTTTCAATAGTGG+GGG | 0.535688 | 6.3:+72166835 | MS.gene37742:CDS |
| TTGTGGATTGTATTTGAACC+CGG | 0.536118 | 6.3:-72167108 | None:intergenic |
| GTATGAAGATGCTTATAAGT+TGG | 0.537055 | 6.3:+72167149 | MS.gene37742:CDS |
| ATAATCAAGGTTTACCGTTG+AGG | 0.542322 | 6.3:+72166914 | MS.gene37742:CDS |
| TTCCACACTAAGGAATGGTT+AGG | 0.542536 | 6.3:-72167027 | None:intergenic |
| GATGTGTCAATGACTTGATC+GGG | 0.551157 | 6.3:-72166742 | None:intergenic |
| TAATGGTGTATTGGGAAACC+CGG | 0.551726 | 6.3:+72167089 | MS.gene37742:CDS |
| CTTTAAAGTTAAGTTTCAGA+AGG | 0.553919 | 6.3:+72167275 | MS.gene37742:CDS |
| GTCCTAACCATTCCTTAGTG+TGG | 0.567409 | 6.3:+72167025 | MS.gene37742:CDS |
| GGCATTGCTCTTGAATCCGT+CGG | 0.567668 | 6.3:+72166856 | MS.gene37742:CDS |
| ACACCCGTTAACAACAAGAA+AGG | 0.576147 | 6.3:+72166940 | MS.gene37742:CDS |
| GCTACGCCATTTACAACATG+TGG | 0.583203 | 6.3:+72166808 | MS.gene37742:CDS |
| GATCAAGTCATTGACACATC+GGG | 0.588958 | 6.3:+72166745 | MS.gene37742:CDS |
| TTCACAACTACTACATCAAG+AGG | 0.592646 | 6.3:-72166889 | None:intergenic |
| CAATCTTCCACACTAAGGAA+TGG | 0.596624 | 6.3:-72167032 | None:intergenic |
| GTGAGGACAATGCAAAGAGA+AGG | 0.598876 | 6.3:-72166690 | None:intergenic |
| TGTGGATTGTATTTGAACCC+GGG | 0.605984 | 6.3:-72167107 | None:intergenic |
| CGATCAAGTCATTGACACAT+CGG | 0.616565 | 6.3:+72166744 | MS.gene37742:CDS |
| TTAACGGGTGTGAACCTCAA+CGG | 0.621698 | 6.3:-72166928 | None:intergenic |
| TGATCCAAGTAGTGGTTGTG+AGG | 0.632530 | 6.3:-72166707 | None:intergenic |
| GCTTCAGCTGATCCAAGTAG+TGG | 0.646579 | 6.3:-72166715 | None:intergenic |
| AATGGTGTATTGGGAAACCC+GGG | 0.666072 | 6.3:+72167090 | MS.gene37742:CDS |
| AAGAGGGCAAGATTCGCCGA+CGG | 0.698733 | 6.3:-72166872 | None:intergenic |
| TCACAACTACTACATCAAGA+GGG | 0.703659 | 6.3:-72166888 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTTATGTGTATAAGACTA+GGG | + | chr6.3:72167224-72167243 | MS.gene37742:CDS | 20.0% |
| !!! | AATGTTTCTTCTTTAGAAAA+AGG | - | chr6.3:72167060-72167079 | None:intergenic | 20.0% |
| !!! | TTCTGAAACTTAACTTTAAA+GGG | - | chr6.3:72167276-72167295 | None:intergenic | 20.0% |
| ! | GAATTTATGTGTATAAGACT+AGG | + | chr6.3:72167223-72167242 | MS.gene37742:CDS | 25.0% |
| !! | TTGTTAGTTGTTTCAATAGT+GGG | + | chr6.3:72166833-72166852 | MS.gene37742:CDS | 25.0% |
| !! | TTTGTTAGTTGTTTCAATAG+TGG | + | chr6.3:72166832-72166851 | MS.gene37742:CDS | 25.0% |
| !!! | CTTCTGAAACTTAACTTTAA+AGG | - | chr6.3:72167277-72167296 | None:intergenic | 25.0% |
| !!! | CTTTAAAGTTAAGTTTCAGA+AGG | + | chr6.3:72167275-72167294 | MS.gene37742:CDS | 25.0% |
| AACCATGTATGCAAAGATAT+TGG | + | chr6.3:72167201-72167220 | MS.gene37742:CDS | 30.0% | |
| CATCTTCATACTTCTCAATT+TGG | - | chr6.3:72167140-72167159 | None:intergenic | 30.0% | |
| GAAACATTTGTGACTACTAA+TGG | + | chr6.3:72167072-72167091 | MS.gene37742:CDS | 30.0% | |
| GTAGTTGTGAAACATAATCA+AGG | + | chr6.3:72166901-72166920 | MS.gene37742:CDS | 30.0% | |
| GTATGAAGATGCTTATAAGT+TGG | + | chr6.3:72167149-72167168 | MS.gene37742:CDS | 30.0% | |
| GTTCAAATACAATCCACAAT+TGG | + | chr6.3:72167112-72167131 | MS.gene37742:CDS | 30.0% | |
| TACCAATTACTACATCATTC+CGG | + | chr6.3:72166786-72166805 | MS.gene37742:CDS | 30.0% | |
| TTCCAATATCTTTGCATACA+TGG | - | chr6.3:72167206-72167225 | None:intergenic | 30.0% | |
| ! | TGTTAGTTGTTTCAATAGTG+GGG | + | chr6.3:72166834-72166853 | MS.gene37742:CDS | 30.0% |
| ACAAAGCCACATGTTGTAAA+TGG | - | chr6.3:72166817-72166836 | None:intergenic | 35.0% | |
| ATAATCAAGGTTTACCGTTG+AGG | + | chr6.3:72166914-72166933 | MS.gene37742:CDS | 35.0% | |
| GAACTTTATGTTGAGATCAG+TGG | - | chr6.3:72166980-72166999 | None:intergenic | 35.0% | |
| GTGACTACTAATGGTGTATT+GGG | + | chr6.3:72167081-72167100 | MS.gene37742:CDS | 35.0% | |
| TCACAACTACTACATCAAGA+GGG | - | chr6.3:72166891-72166910 | None:intergenic | 35.0% | |
| TGTGACTACTAATGGTGTAT+TGG | + | chr6.3:72167080-72167099 | MS.gene37742:CDS | 35.0% | |
| TTCACAACTACTACATCAAG+AGG | - | chr6.3:72166892-72166911 | None:intergenic | 35.0% | |
| ! | GTTAGTTGTTTCAATAGTGG+GGG | + | chr6.3:72166835-72166854 | MS.gene37742:CDS | 35.0% |
| ! | TCTCAATTTGGAACCAATTG+TGG | - | chr6.3:72167128-72167147 | None:intergenic | 35.0% |
| ! | TTGTGGATTGTATTTGAACC+CGG | - | chr6.3:72167111-72167130 | None:intergenic | 35.0% |
| !! | AACGCCTTTCTTGTTGTTAA+CGG | - | chr6.3:72166947-72166966 | None:intergenic | 35.0% |
| ACACCCGTTAACAACAAGAA+AGG | + | chr6.3:72166940-72166959 | MS.gene37742:CDS | 40.0% | |
| AGCCGGAATGATGTAGTAAT+TGG | - | chr6.3:72166791-72166810 | None:intergenic | 40.0% | |
| AGGATCAATCTTCCACACTA+AGG | - | chr6.3:72167040-72167059 | None:intergenic | 40.0% | |
| CAATCTTCCACACTAAGGAA+TGG | - | chr6.3:72167035-72167054 | None:intergenic | 40.0% | |
| CGATCAAGTCATTGACACAT+CGG | + | chr6.3:72166744-72166763 | MS.gene37742:CDS | 40.0% | |
| CGATGTGTCAATGACTTGAT+CGG | - | chr6.3:72166746-72166765 | None:intergenic | 40.0% | |
| GATCAAGTCATTGACACATC+GGG | + | chr6.3:72166745-72166764 | MS.gene37742:CDS | 40.0% | |
| GATGTGTCAATGACTTGATC+GGG | - | chr6.3:72166745-72166764 | None:intergenic | 40.0% | |
| TAAGACTAGGGAAATGCGTT+TGG | + | chr6.3:72167236-72167255 | MS.gene37742:CDS | 40.0% | |
| TAATGGTGTATTGGGAAACC+CGG | + | chr6.3:72167089-72167108 | MS.gene37742:CDS | 40.0% | |
| TGCATACATGGTTGCAAGTA+GGG | - | chr6.3:72167194-72167213 | None:intergenic | 40.0% | |
| TTCCACACTAAGGAATGGTT+AGG | - | chr6.3:72167030-72167049 | None:intergenic | 40.0% | |
| TTGCATACATGGTTGCAAGT+AGG | - | chr6.3:72167195-72167214 | None:intergenic | 40.0% | |
| ! | TGTGGATTGTATTTGAACCC+GGG | - | chr6.3:72167110-72167129 | None:intergenic | 40.0% |
| !! | ACGCCTTTCTTGTTGTTAAC+GGG | - | chr6.3:72166946-72166965 | None:intergenic | 40.0% |
| AATGGTGTATTGGGAAACCC+GGG | + | chr6.3:72167090-72167109 | MS.gene37742:CDS | 45.0% | |
| CATGTTGTAAATGGCGTAGC+CGG | - | chr6.3:72166808-72166827 | None:intergenic | 45.0% | |
| GCTACGCCATTTACAACATG+TGG | + | chr6.3:72166808-72166827 | MS.gene37742:CDS | 45.0% | |
| GTCCTAACCATTCCTTAGTG+TGG | + | chr6.3:72167025-72167044 | MS.gene37742:CDS | 45.0% | |
| GTGAGGACAATGCAAAGAGA+AGG | - | chr6.3:72166693-72166712 | None:intergenic | 45.0% | |
| TGATCCAAGTAGTGGTTGTG+AGG | - | chr6.3:72166710-72166729 | None:intergenic | 45.0% | |
| TTGCAAGTAGGGCACACATT+AGG | - | chr6.3:72167183-72167202 | None:intergenic | 45.0% | |
| TTGTCCTCACAACCACTACT+TGG | + | chr6.3:72166703-72166722 | MS.gene37742:CDS | 45.0% | |
| ! | TTAACGGGTGTGAACCTCAA+CGG | - | chr6.3:72166931-72166950 | None:intergenic | 45.0% |
| GCTTCAGCTGATCCAAGTAG+TGG | - | chr6.3:72166718-72166737 | None:intergenic | 50.0% | |
| GGCATTGCTCTTGAATCCGT+CGG | + | chr6.3:72166856-72166875 | MS.gene37742:CDS | 50.0% | |
| AAGAGGGCAAGATTCGCCGA+CGG | - | chr6.3:72166875-72166894 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 72166664 | 72167302 | 72166664 | ID=MS.gene37742 |
| chr6.3 | mRNA | 72166664 | 72167302 | 72166664 | ID=MS.gene37742.t1;Parent=MS.gene37742 |
| chr6.3 | exon | 72166664 | 72167302 | 72166664 | ID=MS.gene37742.t1.exon1;Parent=MS.gene37742.t1 |
| chr6.3 | CDS | 72166664 | 72167302 | 72166664 | ID=cds.MS.gene37742.t1;Parent=MS.gene37742.t1 |
| Gene Sequence |
| Protein sequence |
