Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37990.t1 | GAU34100.1 | 72.5 | 69 | 19 | 0 | 1 | 69 | 1 | 69 | 2.40E-13 | 84.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37990.t1 | G7K4R6 | 100.0 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | 1.7e-13 | 84.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049887 | MS.gene37990 | 0.803269 | 3.78E-49 | -1.69E-46 |
| MS.gene051063 | MS.gene37990 | 0.804186 | 2.44E-49 | -1.69E-46 |
| MS.gene051687 | MS.gene37990 | 0.804219 | 2.40E-49 | -1.69E-46 |
| MS.gene051748 | MS.gene37990 | 0.804045 | 2.61E-49 | -1.69E-46 |
| MS.gene051903 | MS.gene37990 | 0.811533 | 6.68E-51 | -1.69E-46 |
| MS.gene052242 | MS.gene37990 | -0.805538 | 1.27E-49 | -1.69E-46 |
| MS.gene055174 | MS.gene37990 | 0.812749 | 3.63E-51 | -1.69E-46 |
| MS.gene056830 | MS.gene37990 | -0.821532 | 3.84E-53 | -1.69E-46 |
| MS.gene057234 | MS.gene37990 | 0.803434 | 3.49E-49 | -1.69E-46 |
| MS.gene057452 | MS.gene37990 | -0.800043 | 1.73E-48 | -1.69E-46 |
| MS.gene058074 | MS.gene37990 | 0.835021 | 2.14E-56 | -1.69E-46 |
| MS.gene06001 | MS.gene37990 | 0.807664 | 4.53E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37990.t1 | MTR_5g096470 | 100.000 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | 9.08e-43 | 138 |
| MS.gene37990.t1 | MTR_3g060850 | 63.380 | 71 | 14 | 4 | 1 | 69 | 1 | 61 | 1.89e-17 | 72.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37990.t1 | AT4G26550 | 60.294 | 68 | 22 | 2 | 2 | 69 | 3 | 65 | 6.83e-19 | 76.6 |
| MS.gene37990.t1 | AT5G56020 | 53.623 | 69 | 30 | 2 | 2 | 69 | 6 | 73 | 3.93e-16 | 69.3 |
| MS.gene37990.t1 | AT5G56020 | 53.623 | 69 | 30 | 2 | 2 | 69 | 6 | 73 | 3.93e-16 | 69.3 |
Find 12 sgRNAs with CRISPR-Local
Find 11 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCTTCTTTGCTTGCTGAT+TGG | 0.228357 | 5.1:+76888913 | MS.gene37990:CDS |
| AGTGCCGGTGACGGTGTTAT+TGG | 0.374460 | 5.1:-76889018 | None:intergenic |
| CTCATATGCTTCATCTCAAC+AGG | 0.394643 | 5.1:+76888938 | MS.gene37990:CDS |
| TGCTTCATCTCAACAGGATC+AGG | 0.472082 | 5.1:+76888944 | MS.gene37990:CDS |
| AAGCTGATGGCGGAATTAGA+AGG | 0.478951 | 5.1:-76888969 | None:intergenic |
| GATGATCAGCAACAGAAAGC+AGG | 0.553186 | 5.1:+76888879 | MS.gene37990:CDS |
| TCTGCCAATAACACCGTCAC+CGG | 0.555327 | 5.1:+76889014 | MS.gene37990:CDS |
| AGGTTACACGCTGAAAGTGC+CGG | 0.584313 | 5.1:-76889033 | None:intergenic |
| CACGCTGAAAGTGCCGGTGA+CGG | 0.591513 | 5.1:-76889027 | None:intergenic |
| AGATTCAAGATCAAAGCTGA+TGG | 0.611119 | 5.1:-76888982 | None:intergenic |
| TTCAAGATCAAAGCTGATGG+CGG | 0.643759 | 5.1:-76888979 | None:intergenic |
| GATCAGCAACAGAAAGCAGG+AGG | 0.684803 | 5.1:+76888882 | MS.gene37990:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGATTCAAGATCAAAGCTGA+TGG | - | chr5.1:76888985-76889004 | None:intergenic | 35.0% | |
| CTCATATGCTTCATCTCAAC+AGG | + | chr5.1:76888938-76888957 | MS.gene37990:CDS | 40.0% | |
| TTCAAGATCAAAGCTGATGG+CGG | - | chr5.1:76888982-76889001 | None:intergenic | 40.0% | |
| ! | CTTCTTCTTTGCTTGCTGAT+TGG | + | chr5.1:76888913-76888932 | MS.gene37990:CDS | 40.0% |
| GATGATCAGCAACAGAAAGC+AGG | + | chr5.1:76888879-76888898 | MS.gene37990:CDS | 45.0% | |
| TGCTTCATCTCAACAGGATC+AGG | + | chr5.1:76888944-76888963 | MS.gene37990:CDS | 45.0% | |
| !! | AAGCTGATGGCGGAATTAGA+AGG | - | chr5.1:76888972-76888991 | None:intergenic | 45.0% |
| GATCAGCAACAGAAAGCAGG+AGG | + | chr5.1:76888882-76888901 | MS.gene37990:CDS | 50.0% | |
| TCTGCCAATAACACCGTCAC+CGG | + | chr5.1:76889014-76889033 | MS.gene37990:CDS | 50.0% | |
| !! | AGTGCCGGTGACGGTGTTAT+TGG | - | chr5.1:76889021-76889040 | None:intergenic | 55.0% |
| !! | CACGCTGAAAGTGCCGGTGA+CGG | - | chr5.1:76889030-76889049 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 76888843 | 76889052 | 76888843 | ID=MS.gene37990 |
| chr5.1 | mRNA | 76888843 | 76889052 | 76888843 | ID=MS.gene37990.t1;Parent=MS.gene37990 |
| chr5.1 | exon | 76888843 | 76889052 | 76888843 | ID=MS.gene37990.t1.exon1;Parent=MS.gene37990.t1 |
| chr5.1 | CDS | 76888843 | 76889052 | 76888843 | ID=cds.MS.gene37990.t1;Parent=MS.gene37990.t1 |
| Gene Sequence |
| Protein sequence |
