Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37994.t1 | XP_003617886.2 | 92.7 | 302 | 21 | 1 | 1 | 301 | 3 | 304 | 2.60E-157 | 564.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37994.t1 | Q5WVQ1 | 28.8 | 160 | 95 | 3 | 134 | 293 | 4 | 144 | 8.5e-08 | 59.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37994.t1 | G7K4S5 | 92.7 | 302 | 21 | 1 | 1 | 301 | 3 | 304 | 1.9e-157 | 564.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049297 | MS.gene37994 | 0.821568 | 3.77E-53 | -1.69E-46 |
| MS.gene050226 | MS.gene37994 | 0.805749 | 1.15E-49 | -1.69E-46 |
| MS.gene050227 | MS.gene37994 | 0.808631 | 2.82E-50 | -1.69E-46 |
| MS.gene050228 | MS.gene37994 | 0.815606 | 8.48E-52 | -1.69E-46 |
| MS.gene050438 | MS.gene37994 | 0.802251 | 6.13E-49 | -1.69E-46 |
| MS.gene050558 | MS.gene37994 | 0.801385 | 9.23E-49 | -1.69E-46 |
| MS.gene050719 | MS.gene37994 | 0.80593 | 1.05E-49 | -1.69E-46 |
| MS.gene050721 | MS.gene37994 | 0.803952 | 2.73E-49 | -1.69E-46 |
| MS.gene050724 | MS.gene37994 | 0.804032 | 2.62E-49 | -1.69E-46 |
| MS.gene050807 | MS.gene37994 | 0.817504 | 3.19E-52 | -1.69E-46 |
| MS.gene052302 | MS.gene37994 | 0.809407 | 1.92E-50 | -1.69E-46 |
| MS.gene052323 | MS.gene37994 | 0.801272 | 9.73E-49 | -1.69E-46 |
| MS.gene052376 | MS.gene37994 | 0.820705 | 5.96E-53 | -1.69E-46 |
| MS.gene052665 | MS.gene37994 | 0.814402 | 1.57E-51 | -1.69E-46 |
| MS.gene052858 | MS.gene37994 | 0.808111 | 3.64E-50 | -1.69E-46 |
| MS.gene052860 | MS.gene37994 | 0.803112 | 4.07E-49 | -1.69E-46 |
| MS.gene052896 | MS.gene37994 | 0.808442 | 3.09E-50 | -1.69E-46 |
| MS.gene053630 | MS.gene37994 | 0.802947 | 4.41E-49 | -1.69E-46 |
| MS.gene053836 | MS.gene37994 | 0.811881 | 5.61E-51 | -1.69E-46 |
| MS.gene054379 | MS.gene37994 | 0.802343 | 5.87E-49 | -1.69E-46 |
| MS.gene054473 | MS.gene37994 | 0.808006 | 3.83E-50 | -1.69E-46 |
| MS.gene054942 | MS.gene37994 | 0.808834 | 2.55E-50 | -1.69E-46 |
| MS.gene055363 | MS.gene37994 | 0.801092 | 1.06E-48 | -1.69E-46 |
| MS.gene056069 | MS.gene37994 | -0.818649 | 1.76E-52 | -1.69E-46 |
| MS.gene056589 | MS.gene37994 | 0.804875 | 1.75E-49 | -1.69E-46 |
| MS.gene057260 | MS.gene37994 | 0.821019 | 5.04E-53 | -1.69E-46 |
| MS.gene058168 | MS.gene37994 | 0.802796 | 4.73E-49 | -1.69E-46 |
| MS.gene058851 | MS.gene37994 | 0.82602 | 3.41E-54 | -1.69E-46 |
| MS.gene058906 | MS.gene37994 | 0.801352 | 9.38E-49 | -1.69E-46 |
| MS.gene060476 | MS.gene37994 | 0.802231 | 6.19E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37994.t1 | MTR_5g096560 | 92.715 | 302 | 21 | 1 | 1 | 301 | 3 | 304 | 0.0 | 558 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37994.t1 | AT3G13226 | 43.089 | 246 | 117 | 6 | 71 | 294 | 133 | 377 | 1.16e-59 | 195 |
Find 61 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGATAAATGATGGGGAAAT+TGG | 0.151574 | 5.1:-76923614 | MS.gene37994:CDS |
| GGAGAAGAGAAGTCAATTAT+TGG | 0.244523 | 5.1:-76916565 | MS.gene37994:CDS |
| TTAGTTCTTGTGTTGGTGTT+AGG | 0.272309 | 5.1:-76923712 | MS.gene37994:CDS |
| ATCACTGCCTCCACTGCATT+AGG | 0.286004 | 5.1:+76919113 | None:intergenic |
| TCACTGCCTCCACTGCATTA+GGG | 0.318334 | 5.1:+76919114 | None:intergenic |
| TTAGGAACATTTGGAAGTTT+CGG | 0.323492 | 5.1:-76923760 | MS.gene37994:intron |
| GTATGAACTTCATCTGGATT+TGG | 0.326325 | 5.1:+76923560 | None:intergenic |
| AAGGACAACAATGATTGTTC+TGG | 0.357621 | 5.1:-76916586 | MS.gene37994:CDS |
| ACCTTTGAACCACTGCTTTG+AGG | 0.360986 | 5.1:+76916504 | None:intergenic |
| CTTGATTTCCTCGTCTCCTT+CGG | 0.361626 | 5.1:+76916472 | None:intergenic |
| GGTTGGAGATTCTTGTGAAC+AGG | 0.371729 | 5.1:-76920106 | MS.gene37994:CDS |
| CTAGATGGTCTTCGTCTACT+TGG | 0.373265 | 5.1:-76917053 | MS.gene37994:CDS |
| AGCCGATTGTTCAGCATCAT+TGG | 0.373844 | 5.1:+76920059 | None:intergenic |
| CATTGATCATCTTTATGTTC+AGG | 0.374786 | 5.1:-76916527 | MS.gene37994:CDS |
| TTCACCCTGCATGATGTGTT+AGG | 0.382312 | 5.1:+76923683 | None:intergenic |
| TCAGAGGGTTCATCAATGAT+AGG | 0.382601 | 5.1:-76917101 | MS.gene37994:intron |
| AAATTGAAATTAGGAACATT+TGG | 0.383207 | 5.1:-76923769 | MS.gene37994:intron |
| CAAAGCTACAATACTCCAAA+TGG | 0.386271 | 5.1:+76923654 | None:intergenic |
| CTGTCAAATTACTTGCATCT+AGG | 0.394839 | 5.1:-76920038 | MS.gene37994:intron |
| CAGGGTGAAAATTCCCCATT+TGG | 0.401072 | 5.1:-76923669 | MS.gene37994:CDS |
| AACTCAGTATGAACTTCATC+TGG | 0.406135 | 5.1:+76923554 | None:intergenic |
| CTTACCTGTTTGATCCGTCT+TGG | 0.409978 | 5.1:+76917027 | None:intergenic |
| AGATAAATGATGGGGAAATT+GGG | 0.421615 | 5.1:-76923613 | MS.gene37994:CDS |
| GATTCTAGGTTCTGAACAAA+TGG | 0.438649 | 5.1:-76920133 | MS.gene37994:intron |
| TCATAGTAAACAAACTAGAT+AGG | 0.439290 | 5.1:-76916399 | MS.gene37994:CDS |
| CTCTTTGATGATAGTGAAGA+AGG | 0.444371 | 5.1:-76923530 | MS.gene37994:intron |
| TAGATGGTCTTCGTCTACTT+GGG | 0.452942 | 5.1:-76917052 | MS.gene37994:CDS |
| AAAGCTACAATACTCCAAAT+GGG | 0.474481 | 5.1:+76923655 | None:intergenic |
| TACCAATGATGCTGAACAAT+CGG | 0.477064 | 5.1:-76920061 | MS.gene37994:CDS |
| AATCATATTCTCAATCTAGA+TGG | 0.477303 | 5.1:-76917068 | MS.gene37994:CDS |
| AGTTAGCTACATACTGTCTC+TGG | 0.482116 | 5.1:+76919081 | None:intergenic |
| CGAAGGAGACGAGGAAATCA+AGG | 0.488095 | 5.1:-76916471 | MS.gene37994:CDS |
| AGAGACTTTAGTTCTTGTGT+TGG | 0.494152 | 5.1:-76923719 | MS.gene37994:CDS |
| GGGGCCAAGACGGATCAAAC+AGG | 0.494987 | 5.1:-76917031 | MS.gene37994:intron |
| CACTGCCTCCACTGCATTAG+GGG | 0.499282 | 5.1:+76919115 | None:intergenic |
| AGTGTAATCAAGATAAATGA+TGG | 0.507706 | 5.1:-76923623 | MS.gene37994:CDS |
| GCCTCAAAGCAGTGGTTCAA+AGG | 0.522041 | 5.1:-76916505 | MS.gene37994:CDS |
| AGAAGTTTAGCGGGAAACAT+TGG | 0.537917 | 5.1:-76924392 | MS.gene37994:CDS |
| AGATGGTCTTCGTCTACTTG+GGG | 0.547146 | 5.1:-76917051 | MS.gene37994:CDS |
| CTGAACATAAAGATGATCAA+TGG | 0.547886 | 5.1:+76916528 | None:intergenic |
| AGGTTCTGAACAAATGGAAG+AGG | 0.550123 | 5.1:-76920127 | MS.gene37994:CDS |
| TCTGAACAAATGGAAGAGGT+TGG | 0.556064 | 5.1:-76920123 | MS.gene37994:CDS |
| TGGCAGGCACTGTTCAAGAA+AGG | 0.558497 | 5.1:-76916652 | MS.gene37994:intron |
| GTGTAATCAAGATAAATGAT+GGG | 0.558653 | 5.1:-76923622 | MS.gene37994:CDS |
| AACATGGCAAGAAGTTTAGC+GGG | 0.560092 | 5.1:-76924401 | MS.gene37994:CDS |
| ACATTCCTAACACATCATGC+AGG | 0.560654 | 5.1:-76923688 | MS.gene37994:CDS |
| ATGTTCAGGCCTCAAAGCAG+TGG | 0.582693 | 5.1:-76916513 | MS.gene37994:CDS |
| ATTCGATGGCTTCAATATCG+TGG | 0.583859 | 5.1:-76916445 | MS.gene37994:CDS |
| GGAAATCAAGGATTATTCGA+TGG | 0.586491 | 5.1:-76916459 | MS.gene37994:CDS |
| TTCGGATTGAATGCTTCAAG+AGG | 0.601314 | 5.1:-76923742 | MS.gene37994:CDS |
| GTCCCAGTTGTGCACTATGG+AGG | 0.603930 | 5.1:+76916364 | None:intergenic |
| GAACATGGCAAGAAGTTTAG+CGG | 0.604379 | 5.1:-76924402 | None:intergenic |
| GAAATTCTCCCCTAATGCAG+TGG | 0.612114 | 5.1:-76919123 | MS.gene37994:CDS |
| AGAATGTTCCGAAGGAGACG+AGG | 0.634818 | 5.1:-76916480 | MS.gene37994:CDS |
| ATTCTCCCCTAATGCAGTGG+AGG | 0.636527 | 5.1:-76919120 | MS.gene37994:CDS |
| TGTAATCAAGATAAATGATG+GGG | 0.644079 | 5.1:-76923621 | MS.gene37994:CDS |
| CAAAGGTCAGAATGTTCCGA+AGG | 0.644420 | 5.1:-76916488 | MS.gene37994:CDS |
| GATGGTCTTCGTCTACTTGG+GGG | 0.660488 | 5.1:-76917050 | MS.gene37994:CDS |
| CATTCCTAACACATCATGCA+GGG | 0.662603 | 5.1:-76923687 | MS.gene37994:CDS |
| AAGCTACAATACTCCAAATG+GGG | 0.674118 | 5.1:+76923656 | None:intergenic |
| TTTGGAGAAGTTTCACAATG+CGG | 0.677880 | 5.1:+76923578 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 76916378 | 76924420 | 76916378 | ID=MS.gene37994 |
| chr5.1 | mRNA | 76916378 | 76924420 | 76916378 | ID=MS.gene37994.t1;Parent=MS.gene37994 |
| chr5.1 | exon | 76924344 | 76924420 | 76924344 | ID=MS.gene37994.t1.exon1;Parent=MS.gene37994.t1 |
| chr5.1 | CDS | 76924344 | 76924420 | 76924344 | ID=cds.MS.gene37994.t1;Parent=MS.gene37994.t1 |
| chr5.1 | exon | 76923531 | 76923778 | 76923531 | ID=MS.gene37994.t1.exon2;Parent=MS.gene37994.t1 |
| chr5.1 | CDS | 76923531 | 76923778 | 76923531 | ID=cds.MS.gene37994.t1;Parent=MS.gene37994.t1 |
| chr5.1 | exon | 76920039 | 76920147 | 76920039 | ID=MS.gene37994.t1.exon3;Parent=MS.gene37994.t1 |
| chr5.1 | CDS | 76920039 | 76920147 | 76920039 | ID=cds.MS.gene37994.t1;Parent=MS.gene37994.t1 |
| chr5.1 | exon | 76919095 | 76919187 | 76919095 | ID=MS.gene37994.t1.exon4;Parent=MS.gene37994.t1 |
| chr5.1 | CDS | 76919095 | 76919187 | 76919095 | ID=cds.MS.gene37994.t1;Parent=MS.gene37994.t1 |
| chr5.1 | exon | 76917032 | 76917119 | 76917032 | ID=MS.gene37994.t1.exon5;Parent=MS.gene37994.t1 |
| chr5.1 | CDS | 76917032 | 76917119 | 76917032 | ID=cds.MS.gene37994.t1;Parent=MS.gene37994.t1 |
| chr5.1 | exon | 76916378 | 76916668 | 76916378 | ID=MS.gene37994.t1.exon6;Parent=MS.gene37994.t1 |
| chr5.1 | CDS | 76916378 | 76916668 | 76916378 | ID=cds.MS.gene37994.t1;Parent=MS.gene37994.t1 |
| Gene Sequence |
| Protein sequence |
