Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055363.t1 | XP_013443746.1 | 95.3 | 403 | 19 | 0 | 1 | 403 | 162 | 564 | 7.80E-226 | 792.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055363.t1 | Q9M219 | 46.0 | 402 | 214 | 2 | 1 | 401 | 155 | 554 | 7.2e-103 | 375.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055363.t1 | A0A072TKN3 | 95.3 | 403 | 19 | 0 | 1 | 403 | 162 | 564 | 5.6e-226 | 792.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene055363.t1 | TR | mTERF |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene055363 | 0.827698 | 1.36E-54 | -1.69E-46 |
| MS.gene050226 | MS.gene055363 | 0.813045 | 3.12E-51 | -1.69E-46 |
| MS.gene050227 | MS.gene055363 | 0.812165 | 4.86E-51 | -1.69E-46 |
| MS.gene050228 | MS.gene055363 | 0.805048 | 1.61E-49 | -1.69E-46 |
| MS.gene050719 | MS.gene055363 | 0.801982 | 6.96E-49 | -1.69E-46 |
| MS.gene050721 | MS.gene055363 | 0.80559 | 1.24E-49 | -1.69E-46 |
| MS.gene050724 | MS.gene055363 | 0.804459 | 2.14E-49 | -1.69E-46 |
| MS.gene050815 | MS.gene055363 | 0.80008 | 1.70E-48 | -1.69E-46 |
| MS.gene051210 | MS.gene055363 | 0.807076 | 6.03E-50 | -1.69E-46 |
| MS.gene052020 | MS.gene055363 | 0.804294 | 2.31E-49 | -1.69E-46 |
| MS.gene052323 | MS.gene055363 | 0.813517 | 2.46E-51 | -1.69E-46 |
| MS.gene052611 | MS.gene055363 | 0.834456 | 2.97E-56 | -1.69E-46 |
| MS.gene052612 | MS.gene055363 | 0.807724 | 4.40E-50 | -1.69E-46 |
| MS.gene052863 | MS.gene055363 | 0.839471 | 1.55E-57 | -1.69E-46 |
| MS.gene052896 | MS.gene055363 | 0.834175 | 3.49E-56 | -1.69E-46 |
| MS.gene053008 | MS.gene055363 | 0.823689 | 1.21E-53 | -1.69E-46 |
| MS.gene055363 | MS.gene060683 | 0.818942 | 1.51E-52 | -1.69E-46 |
| MS.gene055363 | MS.gene060685 | 0.815648 | 8.30E-52 | -1.69E-46 |
| MS.gene055363 | MS.gene060803 | 0.800721 | 1.26E-48 | -1.69E-46 |
| MS.gene055363 | MS.gene061333 | 0.803237 | 3.84E-49 | -1.69E-46 |
| MS.gene055363 | MS.gene061341 | 0.831834 | 1.33E-55 | -1.69E-46 |
| MS.gene055363 | MS.gene06303 | 0.802008 | 6.88E-49 | -1.69E-46 |
| MS.gene055363 | MS.gene064070 | 0.83294 | 7.10E-56 | -1.69E-46 |
| MS.gene055363 | MS.gene064944 | 0.823023 | 1.73E-53 | -1.69E-46 |
| MS.gene055363 | MS.gene065064 | 0.800274 | 1.56E-48 | -1.69E-46 |
| MS.gene055363 | MS.gene065568 | 0.822085 | 2.86E-53 | -1.69E-46 |
| MS.gene055363 | MS.gene066896 | 0.800238 | 1.58E-48 | -1.69E-46 |
| MS.gene055363 | MS.gene06705 | 0.818316 | 2.09E-52 | -1.69E-46 |
| MS.gene055363 | MS.gene067450 | 0.801048 | 1.08E-48 | -1.69E-46 |
| MS.gene055363 | MS.gene067903 | 0.805011 | 1.64E-49 | -1.69E-46 |
| MS.gene055363 | MS.gene067974 | 0.810111 | 1.36E-50 | -1.69E-46 |
| MS.gene055363 | MS.gene067982 | 0.804948 | 1.69E-49 | -1.69E-46 |
| MS.gene055363 | MS.gene068038 | 0.80175 | 7.77E-49 | -1.69E-46 |
| MS.gene055363 | MS.gene068777 | 0.802525 | 5.38E-49 | -1.69E-46 |
| MS.gene055363 | MS.gene06921 | 0.811209 | 7.85E-51 | -1.69E-46 |
| MS.gene055363 | MS.gene06922 | 0.823158 | 1.61E-53 | -1.69E-46 |
| MS.gene055363 | MS.gene069754 | 0.81563 | 8.38E-52 | -1.69E-46 |
| MS.gene055363 | MS.gene070943 | 0.805468 | 1.32E-49 | -1.69E-46 |
| MS.gene055363 | MS.gene073753 | 0.823113 | 1.65E-53 | -1.69E-46 |
| MS.gene055363 | MS.gene07530 | 0.812587 | 3.93E-51 | -1.69E-46 |
| MS.gene055363 | MS.gene07733 | 0.802707 | 4.94E-49 | -1.69E-46 |
| MS.gene055363 | MS.gene08853 | 0.814833 | 1.26E-51 | -1.69E-46 |
| MS.gene055363 | MS.gene09240 | 0.812853 | 3.44E-51 | -1.69E-46 |
| MS.gene055363 | MS.gene20635 | 0.801795 | 7.61E-49 | -1.69E-46 |
| MS.gene055363 | MS.gene24741 | 0.803078 | 4.14E-49 | -1.69E-46 |
| MS.gene055363 | MS.gene24908 | 0.812442 | 4.23E-51 | -1.69E-46 |
| MS.gene055363 | MS.gene25987 | 0.827557 | 1.46E-54 | -1.69E-46 |
| MS.gene055363 | MS.gene27414 | 0.801791 | 7.62E-49 | -1.69E-46 |
| MS.gene055363 | MS.gene27571 | 0.8057 | 1.18E-49 | -1.69E-46 |
| MS.gene055363 | MS.gene27903 | 0.808624 | 2.83E-50 | -1.69E-46 |
| MS.gene055363 | MS.gene28606 | 0.80901 | 2.34E-50 | -1.69E-46 |
| MS.gene055363 | MS.gene29022 | 0.806689 | 7.28E-50 | -1.69E-46 |
| MS.gene055363 | MS.gene29095 | 0.825777 | 3.90E-54 | -1.69E-46 |
| MS.gene055363 | MS.gene29380 | 0.829177 | 5.96E-55 | -1.69E-46 |
| MS.gene055363 | MS.gene29773 | 0.809874 | 1.53E-50 | -1.69E-46 |
| MS.gene055363 | MS.gene30739 | 0.836846 | 7.36E-57 | -1.69E-46 |
| MS.gene055363 | MS.gene31479 | 0.804068 | 2.58E-49 | -1.69E-46 |
| MS.gene055363 | MS.gene35004 | 0.800273 | 1.56E-48 | -1.69E-46 |
| MS.gene055363 | MS.gene35631 | 0.819224 | 1.30E-52 | -1.69E-46 |
| MS.gene055363 | MS.gene36281 | 0.800474 | 1.42E-48 | -1.69E-46 |
| MS.gene055363 | MS.gene37994 | 0.801092 | 1.06E-48 | -1.69E-46 |
| MS.gene055363 | MS.gene38494 | 0.818762 | 1.65E-52 | -1.69E-46 |
| MS.gene055363 | MS.gene39045 | 0.829511 | 4.94E-55 | -1.69E-46 |
| MS.gene055363 | MS.gene39433 | 0.801808 | 7.56E-49 | -1.69E-46 |
| MS.gene055363 | MS.gene42160 | 0.800857 | 1.18E-48 | -1.69E-46 |
| MS.gene055363 | MS.gene43973 | 0.816509 | 5.33E-52 | -1.69E-46 |
| MS.gene055363 | MS.gene45193 | 0.827218 | 1.77E-54 | -1.69E-46 |
| MS.gene055363 | MS.gene45269 | 0.817915 | 2.57E-52 | -1.69E-46 |
| MS.gene055363 | MS.gene46250 | 0.809095 | 2.24E-50 | -1.69E-46 |
| MS.gene055363 | MS.gene48577 | 0.830348 | 3.09E-55 | -1.69E-46 |
| MS.gene055363 | MS.gene49003 | 0.812407 | 4.31E-51 | -1.69E-46 |
| MS.gene055363 | MS.gene49282 | 0.801863 | 7.37E-49 | -1.69E-46 |
| MS.gene055363 | MS.gene49486 | 0.836441 | 9.34E-57 | -1.69E-46 |
| MS.gene055363 | MS.gene49665 | 0.810131 | 1.34E-50 | -1.69E-46 |
| MS.gene055363 | MS.gene51459 | 0.807633 | 4.59E-50 | -1.69E-46 |
| MS.gene055363 | MS.gene51850 | 0.804768 | 1.84E-49 | -1.69E-46 |
| MS.gene055363 | MS.gene52851 | 0.820953 | 5.22E-53 | -1.69E-46 |
| MS.gene055363 | MS.gene52855 | 0.80387 | 2.84E-49 | -1.69E-46 |
| MS.gene055363 | MS.gene53586 | 0.832444 | 9.42E-56 | -1.69E-46 |
| MS.gene055363 | MS.gene55111 | 0.814512 | 1.48E-51 | -1.69E-46 |
| MS.gene055363 | MS.gene55219 | -0.803665 | 3.13E-49 | -1.69E-46 |
| MS.gene055363 | MS.gene55539 | 0.800187 | 1.62E-48 | -1.69E-46 |
| MS.gene055363 | MS.gene56431 | 0.823805 | 1.14E-53 | -1.69E-46 |
| MS.gene055363 | MS.gene56537 | 0.81078 | 9.72E-51 | -1.69E-46 |
| MS.gene055363 | MS.gene58375 | 0.812724 | 3.67E-51 | -1.69E-46 |
| MS.gene055363 | MS.gene62214 | 0.826903 | 2.10E-54 | -1.69E-46 |
| MS.gene055363 | MS.gene62393 | 0.802137 | 6.47E-49 | -1.69E-46 |
| MS.gene055363 | MS.gene63327 | 0.827898 | 1.21E-54 | -1.69E-46 |
| MS.gene055363 | MS.gene64249 | 0.811504 | 6.77E-51 | -1.69E-46 |
| MS.gene055363 | MS.gene64417 | 0.830242 | 3.28E-55 | -1.69E-46 |
| MS.gene055363 | MS.gene67738 | 0.838289 | 3.14E-57 | -1.69E-46 |
| MS.gene055363 | MS.gene69794 | 0.814233 | 1.71E-51 | -1.69E-46 |
| MS.gene055363 | MS.gene70651 | 0.806226 | 9.12E-50 | -1.69E-46 |
| MS.gene055363 | MS.gene71871 | 0.81492 | 1.20E-51 | -1.69E-46 |
| MS.gene055363 | MS.gene72268 | 0.825948 | 3.55E-54 | -1.69E-46 |
| MS.gene055363 | MS.gene73273 | 0.800577 | 1.35E-48 | -1.69E-46 |
| MS.gene055363 | MS.gene74226 | 0.813321 | 2.72E-51 | -1.69E-46 |
| MS.gene055363 | MS.gene74980 | 0.813293 | 2.75E-51 | -1.69E-46 |
| MS.gene055363 | MS.gene75490 | 0.802115 | 6.54E-49 | -1.69E-46 |
| MS.gene055363 | MS.gene75509 | 0.800037 | 1.74E-48 | -1.69E-46 |
| MS.gene055363 | MS.gene76403 | 0.815248 | 1.02E-51 | -1.69E-46 |
| MS.gene055363 | MS.gene78492 | 0.824238 | 9.00E-54 | -1.69E-46 |
| MS.gene055363 | MS.gene81370 | 0.828885 | 7.01E-55 | -1.69E-46 |
| MS.gene055363 | MS.gene81998 | 0.809956 | 1.46E-50 | -1.69E-46 |
| MS.gene055363 | MS.gene82879 | 0.827816 | 1.27E-54 | -1.69E-46 |
| MS.gene055363 | MS.gene83476 | 0.811192 | 7.92E-51 | -1.69E-46 |
| MS.gene055363 | MS.gene84086 | 0.804481 | 2.12E-49 | -1.69E-46 |
| MS.gene055363 | MS.gene84485 | 0.809173 | 2.16E-50 | -1.69E-46 |
| MS.gene055363 | MS.gene86844 | 0.804321 | 2.29E-49 | -1.69E-46 |
| MS.gene055363 | MS.gene88543 | 0.821763 | 3.40E-53 | -1.69E-46 |
| MS.gene055363 | MS.gene89096 | 0.800703 | 1.27E-48 | -1.69E-46 |
| MS.gene055363 | MS.gene89419 | 0.808288 | 3.33E-50 | -1.69E-46 |
| MS.gene055363 | MS.gene90787 | 0.810903 | 9.15E-51 | -1.69E-46 |
| MS.gene055363 | MS.gene91800 | 0.990218 | 1.34E-181 | -1.69E-46 |
| MS.gene055363 | MS.gene93311 | 0.818481 | 1.92E-52 | -1.69E-46 |
| MS.gene055363 | MS.gene95236 | 0.808327 | 3.27E-50 | -1.69E-46 |
| MS.gene055363 | MS.gene95255 | 0.807369 | 5.23E-50 | -1.69E-46 |
| MS.gene055363 | MS.gene96210 | 0.803452 | 3.46E-49 | -1.69E-46 |
| MS.gene055363 | MS.gene96791 | 0.818724 | 1.69E-52 | -1.69E-46 |
| MS.gene055363 | MS.gene98087 | 0.805134 | 1.55E-49 | -1.69E-46 |
| MS.gene055363 | MS.gene98717 | 0.810315 | 1.23E-50 | -1.69E-46 |
| MS.gene055363 | MS.gene99462 | 0.803643 | 3.16E-49 | -1.69E-46 |
| MS.gene055363 | MS.gene99887 | 0.847319 | 1.25E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055363.t1 | MTR_8g005155 | 95.285 | 403 | 19 | 0 | 1 | 403 | 162 | 564 | 0.0 | 792 |
| MS.gene055363.t1 | MTR_7g080620 | 56.404 | 406 | 168 | 3 | 1 | 400 | 179 | 581 | 5.40e-162 | 473 |
| MS.gene055363.t1 | MTR_5g084810 | 21.914 | 324 | 241 | 5 | 70 | 391 | 262 | 575 | 1.16e-21 | 97.4 |
| MS.gene055363.t1 | MTR_7g093000 | 22.364 | 313 | 221 | 7 | 88 | 393 | 264 | 561 | 4.27e-20 | 92.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055363.t1 | AT3G60400 | 46.020 | 402 | 214 | 2 | 1 | 401 | 155 | 554 | 2.23e-126 | 376 |
| MS.gene055363.t1 | AT4G19650 | 21.696 | 401 | 283 | 7 | 1 | 391 | 205 | 584 | 4.52e-22 | 99.0 |
| MS.gene055363.t1 | AT4G19650 | 21.696 | 401 | 283 | 7 | 1 | 391 | 188 | 567 | 5.64e-22 | 98.6 |
| MS.gene055363.t1 | AT5G06811 | 24.615 | 325 | 219 | 8 | 76 | 390 | 262 | 570 | 8.75e-21 | 94.7 |
| MS.gene055363.t1 | AT5G45113 | 24.088 | 274 | 194 | 5 | 120 | 391 | 145 | 406 | 1.05e-18 | 87.8 |
| MS.gene055363.t1 | AT5G06810 | 27.801 | 241 | 150 | 7 | 157 | 390 | 351 | 574 | 2.52e-18 | 87.4 |
Find 77 sgRNAs with CRISPR-Local
Find 108 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTCTAGAGTTTCAGGATTT+TGG | 0.108976 | 8.1:-87133891 | None:intergenic |
| TTTGATTGCCTTCTACATTT+AGG | 0.135769 | 8.1:+87133814 | MS.gene055363:CDS |
| GGGACACTCCTTAGAGCTTT+TGG | 0.239993 | 8.1:+87133942 | MS.gene055363:CDS |
| TAATTTCCTATGTCAGGATA+TGG | 0.244795 | 8.1:+87133921 | MS.gene055363:CDS |
| TCATGAAGCTCGAAATTTAT+TGG | 0.254864 | 8.1:-87134169 | None:intergenic |
| AAACATTTGATTCTTGAGTA+TGG | 0.263491 | 8.1:+87133289 | MS.gene055363:CDS |
| TTGATTGCCTTCTACATTTA+GGG | 0.265986 | 8.1:+87133815 | MS.gene055363:CDS |
| GAAAAGTGGTTCTGAGATAA+AGG | 0.272746 | 8.1:+87132988 | MS.gene055363:CDS |
| TTAAATTTCATGCATACTAT+TGG | 0.285045 | 8.1:+87133718 | MS.gene055363:CDS |
| AATTGCTCAAACCAATGTTT+TGG | 0.304518 | 8.1:-87134141 | None:intergenic |
| CTTTGTTTGTTGAAAAGATA+TGG | 0.308280 | 8.1:+87133016 | MS.gene055363:CDS |
| CATGAAGCTCGAAATTTATT+GGG | 0.313412 | 8.1:-87134168 | None:intergenic |
| ACCTTTATCATGCTCTTCAT+TGG | 0.329479 | 8.1:-87133635 | None:intergenic |
| TTCATGCATACTATTGGCTT+TGG | 0.365567 | 8.1:+87133724 | MS.gene055363:CDS |
| CATGTGATTTAAGATATTCA+TGG | 0.366992 | 8.1:-87133761 | None:intergenic |
| CCCAATGAAGAGCATGATAA+AGG | 0.371844 | 8.1:+87133634 | MS.gene055363:CDS |
| ATCTGAACCTCGGCTTAATT+CGG | 0.372746 | 8.1:-87134002 | None:intergenic |
| GGAGTTGTTGAGTCTCAATT+TGG | 0.376264 | 8.1:+87133399 | MS.gene055363:CDS |
| ATAGGTTGCTTTCTGATTTG+AGG | 0.392968 | 8.1:+87133128 | MS.gene055363:CDS |
| CCTTTATCATGCTCTTCATT+GGG | 0.406357 | 8.1:-87133634 | None:intergenic |
| AAAGATTAATTTCCTATGTC+AGG | 0.406425 | 8.1:+87133915 | MS.gene055363:CDS |
| CCATATGTGGGCTTTAGAAA+AGG | 0.410319 | 8.1:+87134026 | MS.gene055363:CDS |
| GGGCTATGGGTTTACGTGAA+TGG | 0.411227 | 8.1:+87133551 | MS.gene055363:CDS |
| TTATCGATGATGAGGTTGAT+AGG | 0.413270 | 8.1:+87133110 | MS.gene055363:CDS |
| ATGTTTGGTATGATGTTTGT+AGG | 0.414620 | 8.1:+87133200 | MS.gene055363:CDS |
| AAAAGTGTCCAAAAGCTCTA+AGG | 0.419374 | 8.1:-87133950 | None:intergenic |
| ATTCAATCCCTAAATGTAGA+AGG | 0.419800 | 8.1:-87133822 | None:intergenic |
| AGTGATGGTAAGGGGAAGAT+AGG | 0.419950 | 8.1:+87133247 | MS.gene055363:CDS |
| CATATGTGGGCTTTAGAAAA+GGG | 0.423473 | 8.1:+87134027 | MS.gene055363:CDS |
| ACCCATAGCCCTCATCAGAT+TGG | 0.427036 | 8.1:-87133539 | None:intergenic |
| CCCAATCTGATGAGGGCTAT+GGG | 0.435992 | 8.1:+87133538 | MS.gene055363:CDS |
| TTTGTAATGTGCAAATTGTT+GGG | 0.448783 | 8.1:+87133041 | MS.gene055363:CDS |
| GATTTGGGAGGGTGTGTTGA+AGG | 0.450135 | 8.1:+87133169 | MS.gene055363:CDS |
| AATTTCCTATGTCAGGATAT+GGG | 0.450989 | 8.1:+87133922 | MS.gene055363:CDS |
| GGAATGGATTCTAAAGGTCT+TGG | 0.453391 | 8.1:+87133460 | MS.gene055363:CDS |
| TCGGTTGATTCTACAAGGTT+CGG | 0.454814 | 8.1:+87133378 | MS.gene055363:CDS |
| AAGAACTATGCTCATGCGTT+GGG | 0.457899 | 8.1:+87133493 | MS.gene055363:CDS |
| AAAAGTGGTTCTGAGATAAA+GGG | 0.465819 | 8.1:+87132989 | MS.gene055363:CDS |
| TTGAAGGGAATGTGGATGTT+TGG | 0.467882 | 8.1:+87133185 | MS.gene055363:CDS |
| AGAAGAGGAGTTGATTGAAA+AGG | 0.470802 | 8.1:+87133312 | MS.gene055363:CDS |
| CCCATAGCCCTCATCAGATT+GGG | 0.475310 | 8.1:-87133538 | None:intergenic |
| AAACTATTCCATTGCAAGCA+TGG | 0.484064 | 8.1:+87134062 | MS.gene055363:CDS |
| TCCCAATCTGATGAGGGCTA+TGG | 0.486736 | 8.1:+87133537 | MS.gene055363:CDS |
| GATTCTTGAGTATGGAGAAG+AGG | 0.500875 | 8.1:+87133297 | MS.gene055363:CDS |
| CGAGGTTCAGATTCCATATG+TGG | 0.503568 | 8.1:+87134013 | MS.gene055363:CDS |
| ATTTGGGAGGGTGTGTTGAA+GGG | 0.510382 | 8.1:+87133170 | MS.gene055363:CDS |
| GAAGAACTATGCTCATGCGT+TGG | 0.510932 | 8.1:+87133492 | MS.gene055363:CDS |
| TTATGATTTGAGTGATGGTA+AGG | 0.517430 | 8.1:+87133237 | MS.gene055363:CDS |
| AGGGTGTGTTGAAGGGAATG+TGG | 0.519392 | 8.1:+87133177 | MS.gene055363:CDS |
| GGGAAGATAGGTGAACTCAT+TGG | 0.519466 | 8.1:+87133259 | MS.gene055363:CDS |
| CACATTGGAATGGATTCTAA+AGG | 0.534379 | 8.1:+87133454 | MS.gene055363:CDS |
| ACCCGGCTGCTCCAAAACAT+TGG | 0.539925 | 8.1:+87134130 | MS.gene055363:CDS |
| GTTGCTTTCTGATTTGAGGT+TGG | 0.540360 | 8.1:+87133132 | MS.gene055363:CDS |
| TTAAGGCAATTCATGATGCA+AGG | 0.553764 | 8.1:+87133671 | MS.gene055363:CDS |
| GCTGTTGAAACACATTGGAA+TGG | 0.554139 | 8.1:+87133444 | MS.gene055363:CDS |
| CGAATCTTCCCAATCTGATG+AGG | 0.559311 | 8.1:+87133530 | MS.gene055363:CDS |
| CATGATAAAGGTTATCAAAG+TGG | 0.567037 | 8.1:+87133646 | MS.gene055363:CDS |
| TTGTAGATTCAGTGTCGAGA+AGG | 0.567935 | 8.1:+87133345 | MS.gene055363:CDS |
| AGTGTCCCATATCCTGACAT+AGG | 0.569168 | 8.1:-87133927 | None:intergenic |
| AGTCGCAACCATGCTTGCAA+TGG | 0.573982 | 8.1:-87134070 | None:intergenic |
| ATGATTTGAGTGATGGTAAG+GGG | 0.577556 | 8.1:+87133239 | MS.gene055363:CDS |
| AATATCTTAAATCACATGCA+TGG | 0.582398 | 8.1:+87133766 | MS.gene055363:CDS |
| ATAGCTCGGTTGATTCTACA+AGG | 0.584030 | 8.1:+87133373 | MS.gene055363:CDS |
| AATTTACTCATATTGTGAGA+CGG | 0.584295 | 8.1:-87133697 | None:intergenic |
| TATGATTTGAGTGATGGTAA+GGG | 0.587020 | 8.1:+87133238 | MS.gene055363:CDS |
| CATGGTTGCGACTAGTGACA+AGG | 0.592546 | 8.1:+87134080 | MS.gene055363:CDS |
| TAGATTCAGTGTCGAGAAGG+AGG | 0.595864 | 8.1:+87133348 | MS.gene055363:CDS |
| TTGAAGCTGTTGAAACACAT+TGG | 0.621474 | 8.1:+87133439 | MS.gene055363:CDS |
| GAATCTTCCCAATCTGATGA+GGG | 0.621938 | 8.1:+87133531 | MS.gene055363:CDS |
| CACATATGGAATCTGAACCT+CGG | 0.628454 | 8.1:-87134012 | None:intergenic |
| AGATAGGTGAACTCATTGGA+AGG | 0.629844 | 8.1:+87133263 | MS.gene055363:CDS |
| AAGTCAAAACATATAAACGC+CGG | 0.641278 | 8.1:-87133973 | None:intergenic |
| GTGGTTCTGAGATAAAGGGT+AGG | 0.659181 | 8.1:+87132993 | MS.gene055363:CDS |
| TCGAGAAGGAGGAAATAGCT+CGG | 0.662293 | 8.1:+87133359 | MS.gene055363:CDS |
| GAGGTTCAGATTCCATATGT+GGG | 0.667584 | 8.1:+87134014 | MS.gene055363:CDS |
| TAGAGAACCGAATTAAGCCG+AGG | 0.677310 | 8.1:+87133995 | MS.gene055363:CDS |
| ATGGTTGCGACTAGTGACAA+GGG | 0.685800 | 8.1:+87134081 | MS.gene055363:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAAATTTCATGCATACTAT+TGG | + | chr8.1:87133718-87133737 | MS.gene055363:CDS | 20.0% |
| !!! | TTAACTTTTCCTTTTGTTTT+TGG | + | chr8.1:87133070-87133089 | MS.gene055363:CDS | 20.0% |
| ! | AAACATTTGATTCTTGAGTA+TGG | + | chr8.1:87133289-87133308 | MS.gene055363:CDS | 25.0% |
| ! | AAAGATTAATTTCCTATGTC+AGG | + | chr8.1:87133915-87133934 | MS.gene055363:CDS | 25.0% |
| ! | AATATCTTAAATCACATGCA+TGG | + | chr8.1:87133766-87133785 | MS.gene055363:CDS | 25.0% |
| ! | AATTTACTCATATTGTGAGA+CGG | - | chr8.1:87133700-87133719 | None:intergenic | 25.0% |
| ! | TTTGTAATGTGCAAATTGTT+GGG | + | chr8.1:87133041-87133060 | MS.gene055363:CDS | 25.0% |
| !! | CATGTGATTTAAGATATTCA+TGG | - | chr8.1:87133764-87133783 | None:intergenic | 25.0% |
| !! | CTTTGTTTGTTGAAAAGATA+TGG | + | chr8.1:87133016-87133035 | MS.gene055363:CDS | 25.0% |
| !! | TTTTCGATAAAATCAAGGAT+GGG | + | chr8.1:87133575-87133594 | MS.gene055363:CDS | 25.0% |
| !! | TTTTGTAATGTGCAAATTGT+TGG | + | chr8.1:87133040-87133059 | MS.gene055363:CDS | 25.0% |
| !!! | ATGGTTTTTCGATAAAATCA+AGG | + | chr8.1:87133570-87133589 | MS.gene055363:CDS | 25.0% |
| !!! | GTGTTTTTGGATTTTGATTT+GGG | + | chr8.1:87133154-87133173 | MS.gene055363:CDS | 25.0% |
| !!! | TTTTTCGATAAAATCAAGGA+TGG | + | chr8.1:87133574-87133593 | MS.gene055363:CDS | 25.0% |
| AAGTCAAAACATATAAACGC+CGG | - | chr8.1:87133976-87133995 | None:intergenic | 30.0% | |
| AATTTCCTATGTCAGGATAT+GGG | + | chr8.1:87133922-87133941 | MS.gene055363:CDS | 30.0% | |
| ATGTTTGGTATGATGTTTGT+AGG | + | chr8.1:87133200-87133219 | MS.gene055363:CDS | 30.0% | |
| ATTCAATCCCTAAATGTAGA+AGG | - | chr8.1:87133825-87133844 | None:intergenic | 30.0% | |
| CATGAAGCTCGAAATTTATT+GGG | - | chr8.1:87134171-87134190 | None:intergenic | 30.0% | |
| CATGATAAAGGTTATCAAAG+TGG | + | chr8.1:87133646-87133665 | MS.gene055363:CDS | 30.0% | |
| TAATTTCCTATGTCAGGATA+TGG | + | chr8.1:87133921-87133940 | MS.gene055363:CDS | 30.0% | |
| TATGATTTGAGTGATGGTAA+GGG | + | chr8.1:87133238-87133257 | MS.gene055363:CDS | 30.0% | |
| TCATGAAGCTCGAAATTTAT+TGG | - | chr8.1:87134172-87134191 | None:intergenic | 30.0% | |
| TTATGATTTGAGTGATGGTA+AGG | + | chr8.1:87133237-87133256 | MS.gene055363:CDS | 30.0% | |
| TTGATTGCCTTCTACATTTA+GGG | + | chr8.1:87133815-87133834 | MS.gene055363:CDS | 30.0% | |
| TTTGATTGCCTTCTACATTT+AGG | + | chr8.1:87133814-87133833 | MS.gene055363:CDS | 30.0% | |
| ! | AATTGCTCAAACCAATGTTT+TGG | - | chr8.1:87134144-87134163 | None:intergenic | 30.0% |
| ! | GTGTTTTATGATTTGAGTGA+TGG | + | chr8.1:87133232-87133251 | MS.gene055363:CDS | 30.0% |
| !! | AAAAGTGGTTCTGAGATAAA+GGG | + | chr8.1:87132989-87133008 | MS.gene055363:CDS | 30.0% |
| !! | AGGTTATCAAAGTGGTTTTA+AGG | + | chr8.1:87133654-87133673 | MS.gene055363:CDS | 30.0% |
| !!! | ATCTTTTTCTCTAGAGTTTC+AGG | - | chr8.1:87133901-87133920 | None:intergenic | 30.0% |
| !!! | GGTGTTTTTGGATTTTGATT+TGG | + | chr8.1:87133153-87133172 | MS.gene055363:CDS | 30.0% |
| !!! | TTTTGGATTTTGATTTGGGA+GGG | + | chr8.1:87133158-87133177 | MS.gene055363:CDS | 30.0% |
| !!! | TTTTTGGATTTTGATTTGGG+AGG | + | chr8.1:87133157-87133176 | MS.gene055363:CDS | 30.0% |
| AAACTATTCCATTGCAAGCA+TGG | + | chr8.1:87134062-87134081 | MS.gene055363:CDS | 35.0% | |
| ACCTTTATCATGCTCTTCAT+TGG | - | chr8.1:87133638-87133657 | None:intergenic | 35.0% | |
| ATGATTTGAGTGATGGTAAG+GGG | + | chr8.1:87133239-87133258 | MS.gene055363:CDS | 35.0% | |
| CCTTTATCATGCTCTTCATT+GGG | - | chr8.1:87133637-87133656 | None:intergenic | 35.0% | |
| TGCGTTGGGAACAATAAAAA+TGG | + | chr8.1:87133507-87133526 | MS.gene055363:CDS | 35.0% | |
| TTAAGGCAATTCATGATGCA+AGG | + | chr8.1:87133671-87133690 | MS.gene055363:CDS | 35.0% | |
| TTGAAGCTGTTGAAACACAT+TGG | + | chr8.1:87133439-87133458 | MS.gene055363:CDS | 35.0% | |
| ! | AGAAGAGGAGTTGATTGAAA+AGG | + | chr8.1:87133312-87133331 | MS.gene055363:CDS | 35.0% |
| ! | ATAGGTTGCTTTCTGATTTG+AGG | + | chr8.1:87133128-87133147 | MS.gene055363:CDS | 35.0% |
| ! | CACATTGGAATGGATTCTAA+AGG | + | chr8.1:87133454-87133473 | MS.gene055363:CDS | 35.0% |
| ! | TGGGAATCATAAGCTTTTAG+TGG | + | chr8.1:87133594-87133613 | MS.gene055363:CDS | 35.0% |
| ! | TTATCGATGATGAGGTTGAT+AGG | + | chr8.1:87133110-87133129 | MS.gene055363:CDS | 35.0% |
| !! | AAAAGTGTCCAAAAGCTCTA+AGG | - | chr8.1:87133953-87133972 | None:intergenic | 35.0% |
| !! | CATATGTGGGCTTTAGAAAA+GGG | + | chr8.1:87134027-87134046 | MS.gene055363:CDS | 35.0% |
| !! | CCGGGTGAATTTTAAAAACA+CGG | - | chr8.1:87134116-87134135 | None:intergenic | 35.0% |
| !! | GAAAAGTGGTTCTGAGATAA+AGG | + | chr8.1:87132988-87133007 | MS.gene055363:CDS | 35.0% |
| !! | TTCATGCATACTATTGGCTT+TGG | + | chr8.1:87133724-87133743 | MS.gene055363:CDS | 35.0% |
| !!! | CCGTGTTTTTAAAATTCACC+CGG | + | chr8.1:87134113-87134132 | MS.gene055363:CDS | 35.0% |
| !!! | GGATTTTGGCTTAGAATCTT+TGG | - | chr8.1:87133880-87133899 | None:intergenic | 35.0% |
| !!! | TCTCTAGAGTTTCAGGATTT+TGG | - | chr8.1:87133894-87133913 | None:intergenic | 35.0% |
| !!! | TGATTTGAGGTTGGTGTTTT+TGG | + | chr8.1:87133141-87133160 | MS.gene055363:CDS | 35.0% |
| AAGAACTATGCTCATGCGTT+GGG | + | chr8.1:87133493-87133512 | MS.gene055363:CDS | 40.0% | |
| AGATAGGTGAACTCATTGGA+AGG | + | chr8.1:87133263-87133282 | MS.gene055363:CDS | 40.0% | |
| ATCTGAACCTCGGCTTAATT+CGG | - | chr8.1:87134005-87134024 | None:intergenic | 40.0% | |
| CACATATGGAATCTGAACCT+CGG | - | chr8.1:87134015-87134034 | None:intergenic | 40.0% | |
| GAATCTTCCCAATCTGATGA+GGG | + | chr8.1:87133531-87133550 | MS.gene055363:CDS | 40.0% | |
| GAGGTTCAGATTCCATATGT+GGG | + | chr8.1:87134014-87134033 | MS.gene055363:CDS | 40.0% | |
| GATTCTTGAGTATGGAGAAG+AGG | + | chr8.1:87133297-87133316 | MS.gene055363:CDS | 40.0% | |
| GCTGTTGAAACACATTGGAA+TGG | + | chr8.1:87133444-87133463 | MS.gene055363:CDS | 40.0% | |
| GGAGTTGTTGAGTCTCAATT+TGG | + | chr8.1:87133399-87133418 | MS.gene055363:CDS | 40.0% | |
| TTGAAGGGAATGTGGATGTT+TGG | + | chr8.1:87133185-87133204 | MS.gene055363:CDS | 40.0% | |
| ! | AGAGCTTTTGGACACTTTTC+CGG | + | chr8.1:87133954-87133973 | MS.gene055363:CDS | 40.0% |
| ! | ATAGCTCGGTTGATTCTACA+AGG | + | chr8.1:87133373-87133392 | MS.gene055363:CDS | 40.0% |
| ! | CCCAATGAAGAGCATGATAA+AGG | + | chr8.1:87133634-87133653 | MS.gene055363:CDS | 40.0% |
| ! | GGAATGGATTCTAAAGGTCT+TGG | + | chr8.1:87133460-87133479 | MS.gene055363:CDS | 40.0% |
| ! | GTTGCTTTCTGATTTGAGGT+TGG | + | chr8.1:87133132-87133151 | MS.gene055363:CDS | 40.0% |
| ! | TCGGTTGATTCTACAAGGTT+CGG | + | chr8.1:87133378-87133397 | MS.gene055363:CDS | 40.0% |
| ! | TTGTAGATTCAGTGTCGAGA+AGG | + | chr8.1:87133345-87133364 | MS.gene055363:CDS | 40.0% |
| !! | ACTAGTGACAAGGGTTTTGT+TGG | + | chr8.1:87134090-87134109 | MS.gene055363:CDS | 40.0% |
| !! | CCATATGTGGGCTTTAGAAA+AGG | + | chr8.1:87134026-87134045 | MS.gene055363:CDS | 40.0% |
| !!! | CCTTTTCTAAAGCCCACATA+TGG | - | chr8.1:87134029-87134048 | None:intergenic | 40.0% |
| AGGGGGATTTATCGATGATG+AGG | + | chr8.1:87133102-87133121 | MS.gene055363:CDS | 45.0% | |
| AGTGATGGTAAGGGGAAGAT+AGG | + | chr8.1:87133247-87133266 | MS.gene055363:CDS | 45.0% | |
| AGTGTCCCATATCCTGACAT+AGG | - | chr8.1:87133930-87133949 | None:intergenic | 45.0% | |
| ATCGATAAATCCCCCTTCCT+CGG | - | chr8.1:87133098-87133117 | None:intergenic | 45.0% | |
| ATGGTTGCGACTAGTGACAA+GGG | + | chr8.1:87134081-87134100 | MS.gene055363:CDS | 45.0% | |
| CGAATCTTCCCAATCTGATG+AGG | + | chr8.1:87133530-87133549 | MS.gene055363:CDS | 45.0% | |
| CGAGGTTCAGATTCCATATG+TGG | + | chr8.1:87134013-87134032 | MS.gene055363:CDS | 45.0% | |
| GAAGAACTATGCTCATGCGT+TGG | + | chr8.1:87133492-87133511 | MS.gene055363:CDS | 45.0% | |
| GGGAAGATAGGTGAACTCAT+TGG | + | chr8.1:87133259-87133278 | MS.gene055363:CDS | 45.0% | |
| TAGAGAACCGAATTAAGCCG+AGG | + | chr8.1:87133995-87134014 | MS.gene055363:CDS | 45.0% | |
| TCCTCGGCACCAAAAACAAA+AGG | - | chr8.1:87133082-87133101 | None:intergenic | 45.0% | |
| TCGAGAAGGAGGAAATAGCT+CGG | + | chr8.1:87133359-87133378 | MS.gene055363:CDS | 45.0% | |
| ! | ATTTGGGAGGGTGTGTTGAA+GGG | + | chr8.1:87133170-87133189 | MS.gene055363:CDS | 45.0% |
| ! | TAGATTCAGTGTCGAGAAGG+AGG | + | chr8.1:87133348-87133367 | MS.gene055363:CDS | 45.0% |
| !! | GTGGTTCTGAGATAAAGGGT+AGG | + | chr8.1:87132993-87133012 | MS.gene055363:CDS | 45.0% |
| !!! | AACCAATGTTTTGGAGCAGC+CGG | - | chr8.1:87134135-87134154 | None:intergenic | 45.0% |
| !!! | TCCTTTTGTTTTTGGTGCCG+AGG | + | chr8.1:87133078-87133097 | MS.gene055363:CDS | 45.0% |
| !!! | TTGTTTTTGGTGCCGAGGAA+GGG | + | chr8.1:87133083-87133102 | MS.gene055363:CDS | 45.0% |
| !!! | TTTGTTTTTGGTGCCGAGGA+AGG | + | chr8.1:87133082-87133101 | MS.gene055363:CDS | 45.0% |
| ACCCATAGCCCTCATCAGAT+TGG | - | chr8.1:87133542-87133561 | None:intergenic | 50.0% | |
| AGGGTGTGTTGAAGGGAATG+TGG | + | chr8.1:87133177-87133196 | MS.gene055363:CDS | 50.0% | |
| AGTCGCAACCATGCTTGCAA+TGG | - | chr8.1:87134073-87134092 | None:intergenic | 50.0% | |
| CATGGTTGCGACTAGTGACA+AGG | + | chr8.1:87134080-87134099 | MS.gene055363:CDS | 50.0% | |
| CCCAATCTGATGAGGGCTAT+GGG | + | chr8.1:87133538-87133557 | MS.gene055363:CDS | 50.0% | |
| CCCATAGCCCTCATCAGATT+GGG | - | chr8.1:87133541-87133560 | None:intergenic | 50.0% | |
| GGGCTATGGGTTTACGTGAA+TGG | + | chr8.1:87133551-87133570 | MS.gene055363:CDS | 50.0% | |
| TCCCAATCTGATGAGGGCTA+TGG | + | chr8.1:87133537-87133556 | MS.gene055363:CDS | 50.0% | |
| ! | GATTTGGGAGGGTGTGTTGA+AGG | + | chr8.1:87133169-87133188 | MS.gene055363:CDS | 50.0% |
| ! | GGGACACTCCTTAGAGCTTT+TGG | + | chr8.1:87133942-87133961 | MS.gene055363:CDS | 50.0% |
| !!! | ACCAATGTTTTGGAGCAGCC+GGG | - | chr8.1:87134134-87134153 | None:intergenic | 50.0% |
| !!! | TGTTTTTGGTGCCGAGGAAG+GGG | + | chr8.1:87133084-87133103 | MS.gene055363:CDS | 50.0% |
| ACCCGGCTGCTCCAAAACAT+TGG | + | chr8.1:87134130-87134149 | MS.gene055363:CDS | 55.0% | |
| !!! | GTTTTTGGTGCCGAGGAAGG+GGG | + | chr8.1:87133085-87133104 | MS.gene055363:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 87132980 | 87134191 | 87132980 | ID=MS.gene055363 |
| chr8.1 | mRNA | 87132980 | 87134191 | 87132980 | ID=MS.gene055363.t1;Parent=MS.gene055363 |
| chr8.1 | exon | 87132980 | 87134191 | 87132980 | ID=MS.gene055363.t1.exon1;Parent=MS.gene055363.t1 |
| chr8.1 | CDS | 87132980 | 87134191 | 87132980 | ID=cds.MS.gene055363.t1;Parent=MS.gene055363.t1 |
| Gene Sequence |
| Protein sequence |
