Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056589.t1 | PNY14751.1 | 84.2 | 38 | 6 | 0 | 3 | 40 | 3 | 40 | 5.40E-09 | 70.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056589.t1 | A0A2K3PHJ3 | 84.2 | 38 | 6 | 0 | 3 | 40 | 3 | 40 | 3.9e-09 | 70.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05016 | MS.gene056589 | 0.806131 | 9.55E-50 | -1.69E-46 |
| MS.gene050807 | MS.gene056589 | 0.828052 | 1.11E-54 | -1.69E-46 |
| MS.gene051180 | MS.gene056589 | 0.800226 | 1.59E-48 | -1.69E-46 |
| MS.gene052665 | MS.gene056589 | 0.839232 | 1.79E-57 | -1.69E-46 |
| MS.gene055512 | MS.gene056589 | 0.802455 | 5.57E-49 | -1.69E-46 |
| MS.gene056069 | MS.gene056589 | -0.811462 | 6.92E-51 | -1.69E-46 |
| MS.gene056589 | MS.gene056643 | 0.820379 | 7.08E-53 | -1.69E-46 |
| MS.gene056589 | MS.gene057060 | 0.808804 | 2.59E-50 | -1.69E-46 |
| MS.gene056589 | MS.gene061893 | 0.812749 | 3.63E-51 | -1.69E-46 |
| MS.gene056589 | MS.gene061947 | 0.807796 | 4.24E-50 | -1.69E-46 |
| MS.gene056589 | MS.gene06236 | 0.809107 | 2.23E-50 | -1.69E-46 |
| MS.gene056589 | MS.gene064944 | 0.843582 | 1.28E-58 | -1.69E-46 |
| MS.gene056589 | MS.gene065227 | 0.815871 | 7.40E-52 | -1.69E-46 |
| MS.gene056589 | MS.gene065825 | 0.804078 | 2.57E-49 | -1.69E-46 |
| MS.gene056589 | MS.gene067461 | 0.815212 | 1.04E-51 | -1.69E-46 |
| MS.gene056589 | MS.gene22481 | 0.802723 | 4.90E-49 | -1.69E-46 |
| MS.gene056589 | MS.gene23105 | 0.828897 | 6.96E-55 | -1.69E-46 |
| MS.gene056589 | MS.gene24268 | 0.825149 | 5.49E-54 | -1.69E-46 |
| MS.gene056589 | MS.gene25229 | 0.816462 | 5.46E-52 | -1.69E-46 |
| MS.gene056589 | MS.gene26972 | 0.832789 | 7.74E-56 | -1.69E-46 |
| MS.gene056589 | MS.gene27302 | 0.805529 | 1.28E-49 | -1.69E-46 |
| MS.gene056589 | MS.gene27918 | 0.820888 | 5.41E-53 | -1.69E-46 |
| MS.gene056589 | MS.gene29136 | 0.810675 | 1.02E-50 | -1.69E-46 |
| MS.gene056589 | MS.gene30964 | 0.80061 | 1.33E-48 | -1.69E-46 |
| MS.gene056589 | MS.gene31148 | 0.800801 | 1.22E-48 | -1.69E-46 |
| MS.gene056589 | MS.gene33564 | 0.800027 | 1.75E-48 | -1.69E-46 |
| MS.gene056589 | MS.gene37994 | 0.804875 | 1.75E-49 | -1.69E-46 |
| MS.gene056589 | MS.gene42101 | 0.81425 | 1.70E-51 | -1.69E-46 |
| MS.gene056589 | MS.gene42160 | 0.80143 | 9.04E-49 | -1.69E-46 |
| MS.gene056589 | MS.gene44135 | -0.809377 | 1.95E-50 | -1.69E-46 |
| MS.gene056589 | MS.gene44540 | 0.802839 | 4.64E-49 | -1.69E-46 |
| MS.gene056589 | MS.gene45193 | 0.801278 | 9.71E-49 | -1.69E-46 |
| MS.gene056589 | MS.gene45498 | 0.800286 | 1.55E-48 | -1.69E-46 |
| MS.gene056589 | MS.gene47875 | 0.817579 | 3.06E-52 | -1.69E-46 |
| MS.gene056589 | MS.gene48321 | 0.809485 | 1.85E-50 | -1.69E-46 |
| MS.gene056589 | MS.gene48701 | 0.806786 | 6.95E-50 | -1.69E-46 |
| MS.gene056589 | MS.gene49486 | 0.806614 | 7.55E-50 | -1.69E-46 |
| MS.gene056589 | MS.gene50621 | 0.805918 | 1.06E-49 | -1.69E-46 |
| MS.gene056589 | MS.gene53586 | 0.822472 | 2.33E-53 | -1.69E-46 |
| MS.gene056589 | MS.gene55127 | 0.802998 | 4.30E-49 | -1.69E-46 |
| MS.gene056589 | MS.gene57160 | -0.805274 | 1.44E-49 | -1.69E-46 |
| MS.gene056589 | MS.gene57162 | -0.806967 | 6.36E-50 | -1.69E-46 |
| MS.gene056589 | MS.gene62682 | 0.846724 | 1.81E-59 | -1.69E-46 |
| MS.gene056589 | MS.gene62720 | 0.81034 | 1.21E-50 | -1.69E-46 |
| MS.gene056589 | MS.gene63327 | 0.814202 | 1.74E-51 | -1.69E-46 |
| MS.gene056589 | MS.gene63902 | 0.817518 | 3.16E-52 | -1.69E-46 |
| MS.gene056589 | MS.gene64101 | 0.802224 | 6.21E-49 | -1.69E-46 |
| MS.gene056589 | MS.gene64249 | 0.802284 | 6.04E-49 | -1.69E-46 |
| MS.gene056589 | MS.gene66923 | 0.801732 | 7.84E-49 | -1.69E-46 |
| MS.gene056589 | MS.gene67785 | 0.806064 | 9.86E-50 | -1.69E-46 |
| MS.gene056589 | MS.gene68877 | 0.806692 | 7.27E-50 | -1.69E-46 |
| MS.gene056589 | MS.gene68959 | 0.814827 | 1.26E-51 | -1.69E-46 |
| MS.gene056589 | MS.gene70819 | 0.80146 | 8.91E-49 | -1.69E-46 |
| MS.gene056589 | MS.gene74226 | 0.827801 | 1.28E-54 | -1.69E-46 |
| MS.gene056589 | MS.gene74980 | 0.803462 | 3.45E-49 | -1.69E-46 |
| MS.gene056589 | MS.gene81998 | 0.80113 | 1.04E-48 | -1.69E-46 |
| MS.gene056589 | MS.gene85949 | 0.810027 | 1.41E-50 | -1.69E-46 |
| MS.gene056589 | MS.gene86987 | 0.814518 | 1.48E-51 | -1.69E-46 |
| MS.gene056589 | MS.gene87037 | 0.806586 | 7.66E-50 | -1.69E-46 |
| MS.gene056589 | MS.gene90649 | 0.806605 | 7.58E-50 | -1.69E-46 |
| MS.gene056589 | MS.gene90689 | 0.818506 | 1.89E-52 | -1.69E-46 |
| MS.gene056589 | MS.gene99887 | 0.805062 | 1.60E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCCGCTGTTCTTGTTTATA+AGG | 0.234085 | 4.2:+37837084 | MS.gene056589:CDS |
| GTTAGTTATAATACTATAAT+TGG | 0.250692 | 4.2:+37836929 | MS.gene056589:CDS |
| ATATGTTTAGAAAGGATTTC+AGG | 0.261501 | 4.2:+37837107 | MS.gene056589:CDS |
| GTTAGGTTACTTTGTGATAA+GGG | 0.340269 | 4.2:+37837157 | MS.gene056589:CDS |
| AACTTATGAGCATCTTGTTA+AGG | 0.349471 | 4.2:+37837036 | MS.gene056589:CDS |
| GGTTAGGTTACTTTGTGATA+AGG | 0.376799 | 4.2:+37837156 | MS.gene056589:CDS |
| ACTTATGAGCATCTTGTTAA+GGG | 0.383733 | 4.2:+37837037 | MS.gene056589:CDS |
| TTGTGATAAGGGTAGGGTTG+AGG | 0.428365 | 4.2:+37837168 | MS.gene056589:CDS |
| TGATGCGTCGACGCTTGATA+TGG | 0.433127 | 4.2:+37837132 | MS.gene056589:CDS |
| ATATCAAGCGTCGACGCATC+AGG | 0.467085 | 4.2:-37837130 | None:intergenic |
| TTATAAGGATATGTTTAGAA+AGG | 0.469550 | 4.2:+37837099 | MS.gene056589:CDS |
| GGGTAGGGTTGAGGAAGCTA+TGG | 0.470489 | 4.2:+37837177 | MS.gene056589:CDS |
| GGTTACTTTGTGATAAGGGT+AGG | 0.471009 | 4.2:+37837161 | MS.gene056589:CDS |
| TTTAGAGAAATGGGATTGGT+TGG | 0.475137 | 4.2:+37836995 | MS.gene056589:CDS |
| TGTGGGAAGAAATGAGGAAG+AGG | 0.476085 | 4.2:+37836889 | None:intergenic |
| AAATGAGGAAGAGGAAATTG+AGG | 0.494114 | 4.2:+37836898 | MS.gene056589:CDS |
| CGACGCTTGATATGGTGGTT+AGG | 0.495256 | 4.2:+37837140 | MS.gene056589:CDS |
| TAAGGGGTATTGTGGTATTG+AGG | 0.500307 | 4.2:+37837054 | MS.gene056589:CDS |
| AGTTATAATACTATAATTGG+TGG | 0.502173 | 4.2:+37836932 | MS.gene056589:CDS |
| GTTACTTTGTGATAAGGGTA+GGG | 0.511374 | 4.2:+37837162 | MS.gene056589:CDS |
| GTTATAATACTATAATTGGT+GGG | 0.520003 | 4.2:+37836933 | MS.gene056589:CDS |
| CATCTTGTTAAGGGGTATTG+TGG | 0.525834 | 4.2:+37837046 | MS.gene056589:CDS |
| AGAGGAAATTGAGGCTGATG+TGG | 0.549411 | 4.2:+37836907 | MS.gene056589:CDS |
| AGAAGATGTGGGAAGAAATG+AGG | 0.594391 | 4.2:+37836883 | None:intergenic |
| CTTATGAGCATCTTGTTAAG+GGG | 0.594928 | 4.2:+37837038 | MS.gene056589:CDS |
| TGCGTCGACGCTTGATATGG+TGG | 0.595911 | 4.2:+37837135 | MS.gene056589:CDS |
| TGGTGATGTTGGAAGAGCTG+AGG | 0.672983 | 4.2:+37836967 | MS.gene056589:CDS |
| ATCCTTATAAACAAGAACAG+CGG | 0.708335 | 4.2:-37837086 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTAGTTATAATACTATAAT+TGG | + | chr4.2:37836929-37836948 | MS.gene056589:CDS | 15.0% |
| !! | AGTTATAATACTATAATTGG+TGG | + | chr4.2:37836932-37836951 | MS.gene056589:CDS | 20.0% |
| !! | GTTATAATACTATAATTGGT+GGG | + | chr4.2:37836933-37836952 | MS.gene056589:CDS | 20.0% |
| !! | TTATAAGGATATGTTTAGAA+AGG | + | chr4.2:37837099-37837118 | MS.gene056589:CDS | 20.0% |
| ! | ATATGTTTAGAAAGGATTTC+AGG | + | chr4.2:37837107-37837126 | MS.gene056589:CDS | 25.0% |
| !!! | ATTTTTTAGAGAAATGGGAT+TGG | + | chr4.2:37836991-37837010 | MS.gene056589:CDS | 25.0% |
| !!! | GAGGAATTTTTTAGAGAAAT+GGG | + | chr4.2:37836986-37837005 | MS.gene056589:CDS | 25.0% |
| !!! | TGAGGAATTTTTTAGAGAAA+TGG | + | chr4.2:37836985-37837004 | MS.gene056589:CDS | 25.0% |
| !!! | TTTTGTAATATTGGTGATGT+TGG | + | chr4.2:37836956-37836975 | MS.gene056589:CDS | 25.0% |
| AACTTATGAGCATCTTGTTA+AGG | + | chr4.2:37837036-37837055 | MS.gene056589:CDS | 30.0% | |
| ACTTATGAGCATCTTGTTAA+GGG | + | chr4.2:37837037-37837056 | MS.gene056589:CDS | 30.0% | |
| ATCCTTATAAACAAGAACAG+CGG | - | chr4.2:37837089-37837108 | None:intergenic | 30.0% | |
| ! | GTTAGGTTACTTTGTGATAA+GGG | + | chr4.2:37837157-37837176 | MS.gene056589:CDS | 30.0% |
| !!! | AGGAAGCTATGGAATTTTTT+AGG | + | chr4.2:37837188-37837207 | MS.gene056589:CDS | 30.0% |
| !!! | ATTGGTGGGTTTTGTAATAT+TGG | + | chr4.2:37836947-37836966 | MS.gene056589:CDS | 30.0% |
| AAATGAGGAAGAGGAAATTG+AGG | + | chr4.2:37836898-37836917 | MS.gene056589:CDS | 35.0% | |
| CTTATGAGCATCTTGTTAAG+GGG | + | chr4.2:37837038-37837057 | MS.gene056589:CDS | 35.0% | |
| ! | ATCCGCTGTTCTTGTTTATA+AGG | + | chr4.2:37837084-37837103 | MS.gene056589:CDS | 35.0% |
| ! | GGTTAGGTTACTTTGTGATA+AGG | + | chr4.2:37837156-37837175 | MS.gene056589:CDS | 35.0% |
| ! | GTTACTTTGTGATAAGGGTA+GGG | + | chr4.2:37837162-37837181 | MS.gene056589:CDS | 35.0% |
| ! | TTTAGAGAAATGGGATTGGT+TGG | + | chr4.2:37836995-37837014 | MS.gene056589:CDS | 35.0% |
| !!! | GCTATGGAATTTTTTAGGAG+TGG | + | chr4.2:37837193-37837212 | MS.gene056589:CDS | 35.0% |
| ! | GGTTACTTTGTGATAAGGGT+AGG | + | chr4.2:37837161-37837180 | MS.gene056589:CDS | 40.0% |
| !! | CATCTTGTTAAGGGGTATTG+TGG | + | chr4.2:37837046-37837065 | MS.gene056589:CDS | 40.0% |
| !! | TAAGGGGTATTGTGGTATTG+AGG | + | chr4.2:37837054-37837073 | MS.gene056589:CDS | 40.0% |
| AGAGGAAATTGAGGCTGATG+TGG | + | chr4.2:37836907-37836926 | MS.gene056589:CDS | 45.0% | |
| TTGTGATAAGGGTAGGGTTG+AGG | + | chr4.2:37837168-37837187 | MS.gene056589:CDS | 45.0% | |
| CGACGCTTGATATGGTGGTT+AGG | + | chr4.2:37837140-37837159 | MS.gene056589:CDS | 50.0% | |
| ! | TGATGCGTCGACGCTTGATA+TGG | + | chr4.2:37837132-37837151 | MS.gene056589:CDS | 50.0% |
| ! | TGGTGATGTTGGAAGAGCTG+AGG | + | chr4.2:37836967-37836986 | MS.gene056589:CDS | 50.0% |
| !! | ATATCAAGCGTCGACGCATC+AGG | - | chr4.2:37837133-37837152 | None:intergenic | 50.0% |
| GGGTAGGGTTGAGGAAGCTA+TGG | + | chr4.2:37837177-37837196 | MS.gene056589:CDS | 55.0% | |
| ! | TGCGTCGACGCTTGATATGG+TGG | + | chr4.2:37837135-37837154 | MS.gene056589:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 37836898 | 37837216 | 37836898 | ID=MS.gene056589 |
| chr4.2 | mRNA | 37836898 | 37837216 | 37836898 | ID=MS.gene056589.t1;Parent=MS.gene056589 |
| chr4.2 | exon | 37836898 | 37837216 | 37836898 | ID=MS.gene056589.t1.exon1;Parent=MS.gene056589.t1 |
| chr4.2 | CDS | 37836898 | 37837216 | 37836898 | ID=cds.MS.gene056589.t1;Parent=MS.gene056589.t1 |
| Gene Sequence |
| Protein sequence |
