AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38477

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38477.t1 MTR_3g056705 92.800 250 18 0 14 263 840 1089 1.29e-157 476
MS.gene38477.t1 MTR_5g094830 71.200 250 61 2 14 263 793 1031 5.54e-116 363
MS.gene38477.t1 MTR_5g033030 70.400 250 69 3 14 263 782 1026 8.02e-114 358
MS.gene38477.t1 MTR_5g094810 69.721 251 63 3 14 263 778 1016 2.86e-113 356
MS.gene38477.t1 MTR_5g033320 67.600 250 75 4 14 263 773 1016 3.91e-107 339
MS.gene38477.t1 MTR_3g056700 56.061 264 108 2 14 270 593 855 2.25e-94 302
MS.gene38477.t1 MTR_3g056675 57.200 250 106 1 14 263 540 788 2.44e-90 291
MS.gene38477.t1 MTR_3g056645 54.839 217 97 1 47 263 1 216 1.71e-73 239
MS.gene38477.t1 MTR_1g069450 46.816 267 124 3 14 266 806 1068 9.03e-67 225
MS.gene38477.t1 MTR_7g098690 44.649 271 128 4 14 266 786 1052 2.41e-66 224
MS.gene38477.t1 MTR_8g080050 40.239 251 127 4 14 263 751 979 4.25e-50 177
MS.gene38477.t1 MTR_8g015970 38.645 251 141 4 14 263 587 825 2.94e-45 163
MS.gene38477.t1 MTR_8g015970 38.645 251 141 4 14 263 583 821 3.42e-45 163
MS.gene38477.t1 MTR_8g015970 38.645 251 141 4 14 263 587 825 3.42e-45 163
MS.gene38477.t1 MTR_8g015970 38.645 251 141 4 14 263 583 821 3.66e-45 162
MS.gene38477.t1 MTR_1g088680 35.060 251 153 3 14 263 813 1054 1.19e-44 161
MS.gene38477.t1 MTR_6g084320 36.255 251 135 5 14 263 749 975 4.26e-43 157
MS.gene38477.t1 MTR_2g436710 35.271 258 154 4 14 270 804 1049 5.87e-43 156
MS.gene38477.t1 MTR_6g084320 36.255 251 135 5 14 263 749 975 7.06e-43 156
MS.gene38477.t1 MTR_8g040620 37.200 250 138 3 14 263 762 992 5.22e-42 154
MS.gene38477.t1 MTR_8g040170 35.968 253 145 4 14 263 572 810 9.46e-42 153
MS.gene38477.t1 MTR_8g040170 35.968 253 145 4 14 263 412 650 1.38e-41 152
MS.gene38477.t1 MTR_8g015980 34.902 255 134 4 14 263 759 986 1.51e-41 152
MS.gene38477.t1 MTR_8g016010 35.039 254 146 4 14 263 114 352 4.55e-41 150
MS.gene38477.t1 MTR_8g061970 32.812 256 163 2 14 269 799 1045 2.11e-40 149
MS.gene38477.t1 MTR_8g016020 36.614 254 132 5 14 263 541 769 3.17e-40 148
MS.gene38477.t1 MTR_8g016070 37.008 254 135 5 14 263 769 1001 6.26e-39 145
MS.gene38477.t1 MTR_2g436730 34.884 258 154 5 14 269 802 1047 8.45e-39 144
MS.gene38477.t1 MTR_6g034270 33.333 252 145 3 14 265 601 829 8.65e-39 144
MS.gene38477.t1 MTR_6g034335 34.921 252 142 5 14 265 788 1017 8.94e-39 144
MS.gene38477.t1 MTR_1g099280 32.540 252 150 3 14 265 782 1013 1.36e-38 144
MS.gene38477.t1 MTR_1g099280 32.540 252 150 3 14 265 807 1038 1.45e-38 144
MS.gene38477.t1 MTR_1g099280 32.540 252 150 3 14 265 782 1013 1.57e-38 144
MS.gene38477.t1 MTR_2g436680 33.984 256 155 3 14 269 804 1045 4.11e-38 142
MS.gene38477.t1 MTR_6g034230 32.540 252 147 4 14 265 784 1012 6.62e-38 142
MS.gene38477.t1 MTR_6g034755 31.225 253 152 3 14 266 791 1021 4.89e-36 136
MS.gene38477.t1 MTR_6g034265 32.806 253 145 4 14 265 782 1010 8.76e-36 135
MS.gene38477.t1 MTR_6g034310 32.937 252 143 5 14 265 412 637 1.84e-35 135
MS.gene38477.t1 MTR_0196s0020 32.016 253 147 3 14 265 784 1012 2.44e-35 134
MS.gene38477.t1 MTR_6g034310 32.937 252 143 5 14 265 770 995 2.76e-35 134
MS.gene38477.t1 MTR_6g034310 32.937 252 143 5 14 265 770 995 2.88e-35 134
MS.gene38477.t1 MTR_0019s0020 32.411 253 146 3 14 265 734 962 1.62e-34 132
MS.gene38477.t1 MTR_0019s0020 32.411 253 146 3 14 265 734 962 1.72e-34 132
MS.gene38477.t1 MTR_0019s0020 32.411 253 146 3 14 265 734 962 2.03e-34 132
MS.gene38477.t1 MTR_0019s0020 32.411 253 146 3 14 265 734 962 2.11e-34 132
MS.gene38477.t1 MTR_0019s0020 32.411 253 146 3 14 265 734 962 2.32e-34 131
MS.gene38477.t1 MTR_6g034350 30.040 253 155 3 14 266 791 1021 2.75e-34 131
MS.gene38477.t1 MTR_6g034220 33.858 254 137 5 16 265 785 1011 5.21e-34 130
MS.gene38477.t1 MTR_3g011840 30.159 252 156 6 14 264 702 934 3.72e-23 99.4
MS.gene38477.t1 MTR_8g042520 29.279 222 131 5 44 265 11 206 4.47e-23 99.0
MS.gene38477.t1 MTR_3g011820 28.175 252 162 6 14 264 818 1051 2.78e-20 90.9
MS.gene38477.t1 MTR_2g019020 27.692 260 156 8 14 264 324 560 1.15e-17 83.2
MS.gene38477.t1 MTR_2g019020 27.692 260 156 8 14 264 779 1015 1.30e-17 83.2
MS.gene38477.t1 MTR_2g019020 27.692 260 156 8 14 264 779 1015 1.30e-17 83.2
MS.gene38477.t1 MTR_2g019020 27.692 260 156 8 14 264 596 832 1.33e-17 82.8
MS.gene38477.t1 MTR_2g019020 27.692 260 156 8 14 264 542 778 1.42e-17 82.8
MS.gene38477.t1 MTR_2g105190 23.577 246 163 6 22 265 794 1016 5.93e-14 72.0
MS.gene38477.t1 MTR_2g105190 23.577 246 163 6 22 265 800 1022 6.26e-14 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38477.t1 AT3G13080 62.000 250 93 2 14 263 803 1050 5.03e-104 327
MS.gene38477.t1 AT3G13080 62.000 250 93 2 14 263 803 1050 5.03e-104 327
MS.gene38477.t1 AT3G13080 62.000 250 93 2 14 263 803 1050 1.25e-102 327
MS.gene38477.t1 AT3G13080 62.000 250 93 2 14 263 803 1050 1.86e-102 327
MS.gene38477.t1 AT3G13080 62.000 250 93 2 14 263 803 1050 2.37e-102 326
MS.gene38477.t1 AT1G71330 59.664 238 94 2 14 251 75 310 6.98e-98 290
MS.gene38477.t1 AT3G13100 60.800 250 84 3 14 263 542 777 1.15e-92 297
MS.gene38477.t1 AT3G13100 60.800 250 84 3 14 263 787 1022 2.55e-92 298
MS.gene38477.t1 AT3G13090 58.964 251 88 4 14 263 764 1000 5.33e-92 297
MS.gene38477.t1 AT1G04120 45.556 270 128 4 14 266 750 1017 1.82e-65 221
MS.gene38477.t1 AT1G04120 45.556 270 128 4 14 266 785 1052 1.91e-65 221
MS.gene38477.t1 AT1G04120 45.556 270 128 4 14 266 785 1052 2.03e-65 221
MS.gene38477.t1 AT3G60160 44.000 250 134 2 14 263 793 1036 3.14e-63 215
MS.gene38477.t1 AT3G60160 44.000 250 134 2 14 263 757 1000 3.34e-63 215
MS.gene38477.t1 AT3G60160 44.000 250 134 2 14 263 793 1036 3.84e-63 214
MS.gene38477.t1 AT3G60970 42.000 250 102 3 14 263 377 583 3.93e-55 191
MS.gene38477.t1 AT2G47800 35.000 260 151 4 14 263 804 1055 1.08e-40 150
MS.gene38477.t1 AT3G62700 34.409 279 152 5 14 263 802 1078 6.18e-39 145
MS.gene38477.t1 AT3G21250 33.935 277 133 5 14 271 767 1012 1.13e-37 141
MS.gene38477.t1 AT3G21250 33.935 277 133 5 14 271 345 590 1.13e-37 141
MS.gene38477.t1 AT3G21250 33.935 277 133 5 14 271 345 590 1.13e-37 141
MS.gene38477.t1 AT3G21250 33.935 277 133 5 14 271 761 1006 1.23e-37 141
MS.gene38477.t1 AT3G59140 31.746 252 146 3 14 265 765 990 6.33e-37 139
MS.gene38477.t1 AT3G21250 31.408 277 129 6 14 271 767 1001 8.79e-31 121
MS.gene38477.t1 AT3G21250 31.408 277 129 6 14 271 761 995 9.76e-31 121
MS.gene38477.t1 AT1G30420 27.027 259 159 7 14 265 779 1014 4.55e-21 93.2
MS.gene38477.t1 AT1G30420 27.027 259 159 7 14 265 779 1014 6.64e-21 92.8
MS.gene38477.t1 AT1G30410 26.667 255 165 7 14 265 779 1014 1.05e-20 92.0
MS.gene38477.t1 AT1G30410 26.667 255 165 7 14 265 779 1014 1.05e-20 92.0
MS.gene38477.t1 AT1G30410 26.667 255 165 7 14 265 779 1014 1.05e-20 92.0
MS.gene38477.t1 AT1G30410 26.667 255 165 7 14 265 782 1017 1.06e-20 92.0
MS.gene38477.t1 AT1G30410 26.667 255 165 7 14 265 664 899 1.17e-20 92.0
MS.gene38477.t1 AT1G30410 26.667 255 165 7 14 265 779 1014 1.22e-20 92.0
MS.gene38477.t1 AT2G34660 25.869 259 168 7 14 264 778 1020 4.74e-20 90.1
MS.gene38477.t1 AT2G34660 25.869 259 168 7 14 264 778 1020 4.74e-20 90.1
MS.gene38477.t1 AT2G34660 25.869 259 168 7 14 264 491 733 5.56e-20 90.1
MS.gene38477.t1 AT1G30400 26.718 262 157 6 14 264 778 1015 2.16e-19 88.2
MS.gene38477.t1 AT1G30400 26.718 262 157 6 14 264 778 1015 2.16e-19 88.2
MS.gene38477.t1 AT2G07680 26.482 253 157 4 14 265 730 954 1.66e-17 82.8
MS.gene38477.t1 AT2G07680 26.482 253 157 4 14 265 721 945 1.70e-17 82.8
MS.gene38477.t1 AT2G07680 26.482 253 157 4 14 265 724 948 1.79e-17 82.8
MS.gene38477.t1 AT2G07680 26.482 253 157 4 14 265 647 871 1.91e-17 82.4
MS.gene38477.t1 AT2G07680 26.482 253 157 4 14 265 746 970 1.94e-17 82.4
MS.gene38477.t1 AT2G07680 26.482 253 157 4 14 265 746 970 1.99e-17 82.4

Find 45 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGTGGAAGAATCCTTAAT+AGG 0.249695 3.4:-62468332 MS.gene38477:CDS
ATGTATGCTTCTTGCCATTC+TGG 0.268968 3.4:-62470044 None:intergenic
TTCAAACTGGAAGCAACTAT+TGG 0.295949 3.4:-62468572 MS.gene38477:CDS
TCTTGAACAAGTTGTCCTTT+TGG 0.301001 3.4:+62468711 None:intergenic
TATAGCAAGATCCTTGTATA+TGG 0.336694 3.4:-62469167 MS.gene38477:intron
CAGATCCACATACCCTATTA+AGG 0.343404 3.4:+62468319 None:intergenic
GAAAGAGAGCAAGGTAAAGT+TGG 0.353443 3.4:-62468684 MS.gene38477:CDS
ACCTGCTGCTGACCTTATCT+TGG 0.368279 3.4:-62469076 MS.gene38477:intron
ATGTTTCAACTTCTTCAAAC+TGG 0.375589 3.4:-62468585 MS.gene38477:CDS
CTTGATTCATCAGATGGATA+TGG 0.375614 3.4:-62468855 MS.gene38477:CDS
AAGTGGAAGAATCCTTAATA+GGG 0.421076 3.4:-62468331 MS.gene38477:intron
AAAGAGAGCAAGGTAAAGTT+GGG 0.433227 3.4:-62468683 MS.gene38477:CDS
CCTGTTGTGATGTACTTATC+TGG 0.444623 3.4:+62468820 None:intergenic
AAATATATCACGACAGCATA+TGG 0.450488 3.4:-62468645 MS.gene38477:CDS
CCAGATAAGTACATCACAAC+AGG 0.477072 3.4:-62468820 MS.gene38477:CDS
AGGAGTGTAGTTCCTCGAAC+AGG 0.499646 3.4:+62468510 None:intergenic
TCTGCACTTGATTCATCAGA+TGG 0.505504 3.4:-62468861 MS.gene38477:CDS
CTATTAAGGATTCTTCCACT+TGG 0.507737 3.4:+62468333 None:intergenic
GCAACTCTTGTTGTCACCGC+TGG 0.508064 3.4:-62468435 MS.gene38477:CDS
GAGAATATCTCACCAAGATA+AGG 0.510895 3.4:+62469064 None:intergenic
CAACAAGAGTTGCTCTAGAT+AGG 0.511129 3.4:+62468445 None:intergenic
CCATGTGTCTCTCTTCCTCT+CGG 0.512974 3.4:-62468795 MS.gene38477:CDS
GCAAGATCCTTGTATATGGC+AGG 0.524807 3.4:-62469163 MS.gene38477:CDS
GACACATAAAGAACTTCCTA+TGG 0.530280 3.4:+62468467 None:intergenic
GAGAAGAAAGAAGTACAGAA+TGG 0.536785 3.4:-62468756 MS.gene38477:CDS
GATGTGGAGCCACCTGTTCG+AGG 0.554467 3.4:-62468522 MS.gene38477:CDS
CCACACCTACATAGACAACT+AGG 0.557019 3.4:+62468490 None:intergenic
AATGACGAGATTGAGCCAAA+AGG 0.564564 3.4:-62468726 MS.gene38477:CDS
AAACATTCCTGCCATATACA+AGG 0.566472 3.4:+62469156 None:intergenic
AACTCCCATCTCAAAAGATG+TGG 0.570549 3.4:-62468538 MS.gene38477:CDS
CAAGAAGAAGAAAGAGAGCA+AGG 0.570892 3.4:-62468693 MS.gene38477:CDS
CCTAGTTGTCTATGTAGGTG+TGG 0.574400 3.4:-62468490 MS.gene38477:CDS
ACACTCCTAGTTGTCTATGT+AGG 0.580169 3.4:-62468495 MS.gene38477:CDS
GATGGGGAAATTACTCAATG+TGG 0.583724 3.4:-62468954 MS.gene38477:CDS
AACTGGAAGCAACTATTGGA+TGG 0.586361 3.4:-62468568 MS.gene38477:CDS
ACCAAGATAAGGTCAGCAGC+AGG 0.588609 3.4:+62469075 None:intergenic
TGTCACAAATGGTGCCAGAA+TGG 0.593816 3.4:+62470030 None:intergenic
AAGAAAGAAGTACAGAATGG+TGG 0.595879 3.4:-62468753 MS.gene38477:CDS
TATAATGATCTACTCAACTC+AGG 0.598290 3.4:-62468927 MS.gene38477:CDS
GTCTATGTAGGTGTGGCCAT+AGG 0.623766 3.4:-62468483 MS.gene38477:CDS
CCGAGAGGAAGAGAGACACA+TGG 0.627029 3.4:+62468795 None:intergenic
AGTGTAGTTCCTCGAACAGG+TGG 0.627973 3.4:+62468513 None:intergenic
ATTTCAGCTATCAATATGAG+TGG 0.654447 3.4:+62468609 None:intergenic
TATATCACGACAGCATATGG+AGG 0.683585 3.4:-62468642 MS.gene38477:CDS
AGTAGTTGTCTTATAACCAG+CGG 0.688846 3.4:+62468419 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGATAAAAAAAAAAATAGA+TGG - chr3.4:62467956-62467975 MS.gene38477:intron 10.0%
!!! TTTTTTTTAATCTCTTAAAA+TGG - chr3.4:62468525-62468544 MS.gene38477:CDS 10.0%
!! AAAAAAAAAAAGATGAAGTT+TGG + chr3.4:62467914-62467933 None:intergenic 15.0%
!! AAAAAAAAAAGATGAAGTTT+GGG + chr3.4:62467913-62467932 None:intergenic 15.0%
!! CATAATTTGAAAAGATAAAT+AGG + chr3.4:62468912-62468931 None:intergenic 15.0%
!! GTTCTCTATAATAATTAAAA+AGG + chr3.4:62468181-62468200 None:intergenic 15.0%
!! AAAAGAATATGCATTTATCA+TGG - chr3.4:62468429-62468448 MS.gene38477:CDS 20.0%
!! ATACATATTATAGATAGTAG+AGG + chr3.4:62468282-62468301 None:intergenic 20.0%
!! ATATCAATGAAATGGTATAT+AGG - chr3.4:62469717-62469736 MS.gene38477:intron 20.0%
!! ATCTTGAAATTATAAAGTGT+CGG + chr3.4:62469911-62469930 None:intergenic 20.0%
!! TACATATTATAGATAGTAGA+GGG + chr3.4:62468281-62468300 None:intergenic 20.0%
!! TTTCGATTGAATATGTAAAT+TGG - chr3.4:62469636-62469655 MS.gene38477:intron 20.0%
!!! CTTATAATTTTATTGCGAAT+TGG + chr3.4:62469942-62469961 None:intergenic 20.0%
!!! TATAGATTGGTTTGAAATAT+TGG - chr3.4:62468036-62468055 MS.gene38477:intron 20.0%
!!! TCAAATTATGCATTGTTTTA+AGG - chr3.4:62468922-62468941 MS.gene38477:CDS 20.0%
!!! TTTTTGTCTTCAAATGTAAA+GGG - chr3.4:62468492-62468511 MS.gene38477:CDS 20.0%
! AACAATATAGCAAACAAAGT+TGG + chr3.4:62468320-62468339 None:intergenic 25.0%
! AAGAAACATAATGAGTGAAT+AGG + chr3.4:62470006-62470025 None:intergenic 25.0%
! CTAATATGTACCTAAAAGAA+CGG - chr3.4:62468243-62468262 MS.gene38477:intron 25.0%
! GACCAATGTATAAAATTTCA+AGG + chr3.4:62469663-62469682 None:intergenic 25.0%
! GGCAAAAAATATCAATGAAA+TGG - chr3.4:62469709-62469728 MS.gene38477:intron 25.0%
! TCCACATATAAAATTTCCAA+AGG - chr3.4:62468385-62468404 MS.gene38477:CDS 25.0%
! TGCTATGATGATATATAGAT+TGG - chr3.4:62468023-62468042 MS.gene38477:intron 25.0%
! TGTGGAATAATTGTATTAGA+TGG + chr3.4:62468371-62468390 None:intergenic 25.0%
! TTTGTTAACTTTGAACGTTA+GGG + chr3.4:62469875-62469894 None:intergenic 25.0%
!! ACGAATTTTATGGAACTTAT+TGG - chr3.4:62469004-62469023 MS.gene38477:intron 25.0%
!! CTTTTTGTCTTCAAATGTAA+AGG - chr3.4:62468491-62468510 MS.gene38477:CDS 25.0%
!! GGTGATAATTTTTCACAAAA+AGG + chr3.4:62468593-62468612 None:intergenic 25.0%
!! GTCCTTGAAATTTTATACAT+TGG - chr3.4:62469658-62469677 MS.gene38477:intron 25.0%
!! TTTCAATTTTACTATGTAGC+TGG - chr3.4:62468125-62468144 MS.gene38477:intron 25.0%
!! TTTTATACATTGGTCAAAAC+AGG - chr3.4:62469668-62469687 MS.gene38477:intron 25.0%
!! TTTTGTTAACTTTGAACGTT+AGG + chr3.4:62469876-62469895 None:intergenic 25.0%
!!! GTTTTAAGGTTATGAAAGAT+GGG - chr3.4:62468936-62468955 MS.gene38477:CDS 25.0%
!!! TGTTTTAAGGTTATGAAAGA+TGG - chr3.4:62468935-62468954 MS.gene38477:CDS 25.0%
!!! TTCTTTTAGGTACATATTAG+AGG + chr3.4:62468243-62468262 None:intergenic 25.0%
!!! TTTTAAGGTTATGAAAGATG+GGG - chr3.4:62468937-62468956 MS.gene38477:CDS 25.0%
AAATATATCACGACAGCATA+TGG - chr3.4:62469262-62469281 MS.gene38477:intron 30.0%
AAGTGGAAGAATCCTTAATA+GGG - chr3.4:62469576-62469595 MS.gene38477:intron 30.0%
AATTTCCAAAGGCAATCTAT+AGG - chr3.4:62468396-62468415 MS.gene38477:CDS 30.0%
ATGTTTCAACTTCTTCAAAC+TGG - chr3.4:62469322-62469341 MS.gene38477:intron 30.0%
ATTGGTACATATAGACTAGT+TGG - chr3.4:62468097-62468116 MS.gene38477:intron 30.0%
ATTGTATTAGATGGAGAAGT+TGG + chr3.4:62468362-62468381 None:intergenic 30.0%
ATTTCAGCTATCAATATGAG+TGG + chr3.4:62469301-62469320 None:intergenic 30.0%
CTCTTAAAATGGCAAAAGAA+GGG - chr3.4:62468536-62468555 MS.gene38477:CDS 30.0%
GAAAGAATTTGTGTCACAAA+TGG + chr3.4:62467891-62467910 None:intergenic 30.0%
GTAGCATCAAAAAATGACAT+AGG + chr3.4:62469553-62469572 None:intergenic 30.0%
TATAATGATCTACTCAACTC+AGG - chr3.4:62468980-62468999 MS.gene38477:intron 30.0%
TATAGCAAGATCCTTGTATA+TGG - chr3.4:62468740-62468759 MS.gene38477:CDS 30.0%
TCTCTTAAAATGGCAAAAGA+AGG - chr3.4:62468535-62468554 MS.gene38477:CDS 30.0%
! TCAATTGTTGCCTTCTAAAA+TGG - chr3.4:62468071-62468090 MS.gene38477:intron 30.0%
!! GCCTTTGGAAATTTTATATG+TGG + chr3.4:62468389-62468408 None:intergenic 30.0%
!! TACCAATTAGCCATTTTAGA+AGG + chr3.4:62468084-62468103 None:intergenic 30.0%
AAACATTCCTGCCATATACA+AGG + chr3.4:62468754-62468773 None:intergenic 35.0%
AAAGAGAGCAAGGTAAAGTT+GGG - chr3.4:62469224-62469243 MS.gene38477:intron 35.0%
AGAATCCTTAATAGGGTATG+TGG - chr3.4:62469583-62469602 MS.gene38477:intron 35.0%
AGTAGTTGTCTTATAACCAG+CGG + chr3.4:62469491-62469510 None:intergenic 35.0%
CAAGTGGAAGAATCCTTAAT+AGG - chr3.4:62469575-62469594 MS.gene38477:intron 35.0%
CTATTAAGGATTCTTCCACT+TGG + chr3.4:62469577-62469596 None:intergenic 35.0%
CTTGATTCATCAGATGGATA+TGG - chr3.4:62469052-62469071 MS.gene38477:intron 35.0%
GACACATAAAGAACTTCCTA+TGG + chr3.4:62469443-62469462 None:intergenic 35.0%
GAGAATATCTCACCAAGATA+AGG + chr3.4:62468846-62468865 None:intergenic 35.0%
TACCTAAAAGAACGGCTTAA+AGG - chr3.4:62468251-62468270 MS.gene38477:intron 35.0%
TTCAAACTGGAAGCAACTAT+TGG - chr3.4:62469335-62469354 MS.gene38477:intron 35.0%
TTCCGTTTATGAAGTTCACT+CGG + chr3.4:62468614-62468633 None:intergenic 35.0%
TTTATCATGGCCTACAATCA+AGG - chr3.4:62468442-62468461 MS.gene38477:CDS 35.0%
! AAGAAAGAAGTACAGAATGG+TGG - chr3.4:62469154-62469173 MS.gene38477:CDS 35.0%
! AGGTTCTCAGTTTTACGAAT+TGG - chr3.4:62469688-62469707 MS.gene38477:intron 35.0%
! GAGAAGAAAGAAGTACAGAA+TGG - chr3.4:62469151-62469170 MS.gene38477:CDS 35.0%
! TCTTGAACAAGTTGTCCTTT+TGG + chr3.4:62469199-62469218 None:intergenic 35.0%
!! TGCCTTCTAAAATGGCTAAT+TGG - chr3.4:62468079-62468098 MS.gene38477:intron 35.0%
!! TGTTTGCTATATTGTTGTCG+TGG - chr3.4:62468324-62468343 MS.gene38477:intron 35.0%
!! TTTTCACAAAAAGGCGTGAA+TGG + chr3.4:62468584-62468603 None:intergenic 35.0%
!!! TTTTTTGATGCTACTCCAAG+TGG - chr3.4:62469559-62469578 MS.gene38477:intron 35.0%
AACTCCCATCTCAAAAGATG+TGG - chr3.4:62469369-62469388 MS.gene38477:intron 40.0%
AACTGGAAGCAACTATTGGA+TGG - chr3.4:62469339-62469358 MS.gene38477:intron 40.0%
AATGACGAGATTGAGCCAAA+AGG - chr3.4:62469181-62469200 MS.gene38477:intron 40.0%
AATTTCCGCATTAGAGGCTA+TGG - chr3.4:62469762-62469781 MS.gene38477:intron 40.0%
ACACGTTATGCCTTGATTGT+AGG + chr3.4:62468455-62468474 None:intergenic 40.0%
ACACTCCTAGTTGTCTATGT+AGG - chr3.4:62469412-62469431 MS.gene38477:intron 40.0%
ATCCTCGAAACTGTAAGCAT+TGG + chr3.4:62469976-62469995 None:intergenic 40.0%
CAAGAAGAAGAAAGAGAGCA+AGG - chr3.4:62469214-62469233 MS.gene38477:intron 40.0%
CACCGAGTGAACTTCATAAA+CGG - chr3.4:62468609-62468628 MS.gene38477:CDS 40.0%
CAGATCCACATACCCTATTA+AGG + chr3.4:62469591-62469610 None:intergenic 40.0%
CCAGATAAGTACATCACAAC+AGG - chr3.4:62469087-62469106 MS.gene38477:CDS 40.0%
CCTGTTGTGATGTACTTATC+TGG + chr3.4:62469090-62469109 None:intergenic 40.0%
CTAAAAGAACGGCTTAAAGG+AGG - chr3.4:62468254-62468273 MS.gene38477:intron 40.0%
CTACACCTATAGATTGCCTT+TGG + chr3.4:62468404-62468423 None:intergenic 40.0%
CTTGTCTCAGAGATTGATGT+GGG + chr3.4:62469809-62469828 None:intergenic 40.0%
GAAAGAGAGCAAGGTAAAGT+TGG - chr3.4:62469223-62469242 MS.gene38477:intron 40.0%
GATGGGGAAATTACTCAATG+TGG - chr3.4:62468953-62468972 MS.gene38477:CDS 40.0%
TACCAATGCTTACAGTTTCG+AGG - chr3.4:62469971-62469990 MS.gene38477:intron 40.0%
TATATCACGACAGCATATGG+AGG - chr3.4:62469265-62469284 MS.gene38477:intron 40.0%
TCCATGAATTTCCGCATTAG+AGG - chr3.4:62469756-62469775 MS.gene38477:intron 40.0%
TCTAATGCGGAAATTCATGG+AGG + chr3.4:62469757-62469776 None:intergenic 40.0%
TCTGCACTTGATTCATCAGA+TGG - chr3.4:62469046-62469065 MS.gene38477:intron 40.0%
! AAAAGGCGTGAATGGATAGA+AGG + chr3.4:62468576-62468595 None:intergenic 40.0%
! AAAGGCGTGAATGGATAGAA+GGG + chr3.4:62468575-62468594 None:intergenic 40.0%
! CAACAAGAGTTGCTCTAGAT+AGG + chr3.4:62469465-62469484 None:intergenic 40.0%
! CAACTCAGGCACGAATTTTA+TGG - chr3.4:62468994-62469013 MS.gene38477:intron 40.0%
! CTCCTTTAAGCCGTTCTTTT+AGG + chr3.4:62468256-62468275 None:intergenic 40.0%
! GCAAGGTAAAGTTGGGTTTT+CGG - chr3.4:62469231-62469250 MS.gene38477:intron 40.0%
! TCTATTTTGTCAACACCGAG+AGG + chr3.4:62469130-62469149 None:intergenic 40.0%
!! CTCTGAGACAAGGTTAATAG+AGG - chr3.4:62469817-62469836 MS.gene38477:intron 40.0%
ACATGCCATAGCCTCTAATG+CGG + chr3.4:62469770-62469789 None:intergenic 45.0%
CCACACCTACATAGACAACT+AGG + chr3.4:62469420-62469439 None:intergenic 45.0%
CCTAGTTGTCTATGTAGGTG+TGG - chr3.4:62469417-62469436 MS.gene38477:intron 45.0%
CCTTGTCTCAGAGATTGATG+TGG + chr3.4:62469810-62469829 None:intergenic 45.0%
GAACGTTAGGGACTATGATG+TGG + chr3.4:62469863-62469882 None:intergenic 45.0%
GATGGAGAAGTTGGAGAATG+TGG + chr3.4:62468353-62468372 None:intergenic 45.0%
GCAAGATCCTTGTATATGGC+AGG - chr3.4:62468744-62468763 MS.gene38477:CDS 45.0%
GCCTCTAATGCGGAAATTCA+TGG + chr3.4:62469760-62469779 None:intergenic 45.0%
! TGTCACAAATGGTGCCAGAA+TGG + chr3.4:62467880-62467899 None:intergenic 45.0%
!! AAGTTGGGTTTTCGGTGTAC+TGG - chr3.4:62469239-62469258 MS.gene38477:intron 45.0%
!! CCACATCAATCTCTGAGACA+AGG - chr3.4:62469807-62469826 MS.gene38477:intron 45.0%
!! TGGCTCCACATCTTTTGAGA+TGG + chr3.4:62469377-62469396 None:intergenic 45.0%
!!! GGCTCCACATCTTTTGAGAT+GGG + chr3.4:62469376-62469395 None:intergenic 45.0%
ACCAAGATAAGGTCAGCAGC+AGG + chr3.4:62468835-62468854 None:intergenic 50.0%
ACCTGCTGCTGACCTTATCT+TGG - chr3.4:62468831-62468850 MS.gene38477:CDS 50.0%
AGTGTAGTTCCTCGAACAGG+TGG + chr3.4:62469397-62469416 None:intergenic 50.0%
CCATGTGTCTCTCTTCCTCT+CGG - chr3.4:62469112-62469131 MS.gene38477:CDS 50.0%
GTCTATGTAGGTGTGGCCAT+AGG - chr3.4:62469424-62469443 MS.gene38477:intron 50.0%
! AGGAGTGTAGTTCCTCGAAC+AGG + chr3.4:62469400-62469419 None:intergenic 50.0%
CCGAGAGGAAGAGAGACACA+TGG + chr3.4:62469115-62469134 None:intergenic 55.0%
! GCAACTCTTGTTGTCACCGC+TGG - chr3.4:62469472-62469491 MS.gene38477:intron 55.0%
GATGTGGAGCCACCTGTTCG+AGG - chr3.4:62469385-62469404 MS.gene38477:intron 60.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 62467867 62470062 62467867 ID=MS.gene38477
chr3.4 mRNA 62467867 62470062 62467867 ID=MS.gene38477.t1;Parent=MS.gene38477
chr3.4 exon 62470036 62470062 62470036 ID=MS.gene38477.t1.exon1;Parent=MS.gene38477.t1
chr3.4 CDS 62470036 62470062 62470036 ID=cds.MS.gene38477.t1;Parent=MS.gene38477.t1
chr3.4 exon 62469077 62469184 62469077 ID=MS.gene38477.t1.exon2;Parent=MS.gene38477.t1
chr3.4 CDS 62469077 62469184 62469077 ID=cds.MS.gene38477.t1;Parent=MS.gene38477.t1
chr3.4 exon 62468332 62468985 62468332 ID=MS.gene38477.t1.exon3;Parent=MS.gene38477.t1
chr3.4 CDS 62468332 62468985 62468332 ID=cds.MS.gene38477.t1;Parent=MS.gene38477.t1
chr3.4 exon 62467867 62467896 62467867 ID=MS.gene38477.t1.exon4;Parent=MS.gene38477.t1
chr3.4 CDS 62467867 62467896 62467867 ID=cds.MS.gene38477.t1;Parent=MS.gene38477.t1
Gene Sequence

>MS.gene38477

ATGCTTCTTGCCATTCTGGCACCATTTGTGACACAAATTCTTTCCCAAACTTCATCTTTTTTTTTTTTTCAAGTGAGAAACTTATGCTTTTGATAAAAAAAAAAATAGATGGATTTCAAATTTCTATGAAGTTTTATTAGATATACATTTCTGAGATGCTATGATGATATATAGATTGGTTTGAAATATTGGAGAAAACTTAACTCAATTGTTGCCTTCTAAAATGGCTAATTGGTACATATAGACTAGTTGGCACATTTTCAATTTTACTATGTAGCTGGCAAGAATCAGAATTGATAATGATAGTTTTTCCTTTTTAATTATTATAGAGAACAGATGAAAATTAAATAGAAATAATGTATTTATTAGTGTTCCTCTAATATGTACCTAAAAGAACGGCTTAAAGGAGGACCCTCTACTATCTATAATATGTATGTCTTGAAAGTGTCACCAACTTTGTTTGCTATATTGTTGTCGTGGTAACCACATTCTCCAACTTCTCCATCTAATACAATTATTCCACATATAAAATTTCCAAAGGCAATCTATAGGTGTAGCTGTCAAAAGAATATGCATTTATCATGGCCTACAATCAAGGCATAACGTGTGTGTTTTATGTGTATTCTTTTTGTCTTCAAATGTAAAGGGTAATTGAGAATTTTTTTTAATCTCTTAAAATGGCAAAAGAAGGGAGTAGACTTTGAACCCTTCTATCCATTCACGCCTTTTTGTGAAAAATTATCACCGAGTGAACTTCATAAACGGAAAATTGAAATCAAAATAATTCAAGTTCAATTTGTTTCACTACTACAAAATATATATTTTACATCAAAATAATTCAGTGCTACATTGTTTCTGAAATGTGTTTTTTCTTATAGCAAGATCCTTGTATATGGCAGGAATGTTTGCTGAAACTTTTAAGTTCGAAGACAGTGATCTATGTCACACATCAAGTAGAGTTCTTACCTGCTGCTGACCTTATCTTGGTGAGATATTCTCTAGTTTATGTCATTAAGTCAAGAAGAGTTTGATTATTTTATTTCCTATTTATCTTTTCAAATTATGCATTGTTTTAAGGTTATGAAAGATGGGGAAATTACTCAATGTGGAAAGTATAATGATCTACTCAACTCAGGCACGAATTTTATGGAACTTATTGGTGCTCATAGAGACGCATTGTCTGCACTTGATTCATCAGATGGATATGGTACAGTATTTGTCCAGATAAGTACATCACAACAGGATCCATGTGTCTCTCTTCCTCTCGGTGTTGACAAAATAGAAGAGAAGAAAGAAGTACAGAATGGTGGAACAAATGACGAGATTGAGCCAAAAGGACAACTTGTTCAAGAAGAAGAAAGAGAGCAAGGTAAAGTTGGGTTTTCGGTGTACTGGAAATATATCACGACAGCATATGGAGGTGCTCTTGTGCCACTCATATTGATAGCTGAAATCATGTTTCAACTTCTTCAAACTGGAAGCAACTATTGGATGGCTTCTTCAACTCCCATCTCAAAAGATGTGGAGCCACCTGTTCGAGGAACTACACTCCTAGTTGTCTATGTAGGTGTGGCCATAGGAAGTTCTTTATGTGTCCTATCTAGAGCAACTCTTGTTGTCACCGCTGGTTATAAGACAACTACTCTACTCTTCAACAAAATGCATCTCTGCATTTTTCGCGCTCCTATGTCATTTTTTGATGCTACTCCAAGTGGAAGAATCCTTAATAGGGTATGTGGATCTGTCTATATATCATTCTCTCTATCTTTTTTCGATTGAATATGTAAATTGGTCCTTGAAATTTTATACATTGGTCAAAACAGGTTCTCAGTTTTACGAATTGGCAAAAAATATCAATGAAATGGTATATAGGCATTCAAAATAGCTCCTCCATGAATTTCCGCATTAGAGGCTATGGCATGTCACATTGTACGCTAATCCCACATCAATCTCTGAGACAAGGTTAATAGAGGTCACAACTTCAATTAACATACCACATCATAGTCCCTAACGTTCAAAGTTAACAAAATGACTGTTTTCACCGACACTTTATAATTTCAAGATTGTATTTTCCAATTCGCAATAAAATTATAAGAACTATTTATACCAATGCTTACAGTTTCGAGGATCAATTTTCCTATTCACTCATTATGTTTCTTAATTTAGATGAATATACGAGTCTTAAATTATCTTTAG
Protein sequence

>MS.gene38477.t1

MLLAILAPFQDPCIWQECLLKLLSSKTVIYVTHQVEFLPAADLILVMKDGEITQCGKYNDLLNSGTNFMELIGAHRDALSALDSSDGYGTVFVQISTSQQDPCVSLPLGVDKIEEKKEVQNGGTNDEIEPKGQLVQEEEREQGKVGFSVYWKYITTAYGGALVPLILIAEIMFQLLQTGSNYWMASSTPISKDVEPPVRGTTLLVVYVGVAIGSSLCVLSRATLVVTAGYKTTTLLFNKMHLCIFRAPMSFFDATPSGRILNRMNIRVLNYL