Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38910.t1 | RHN40752.1 | 100 | 54 | 0 | 0 | 15 | 68 | 74 | 127 | 1.40E-21 | 111.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38910.t1 | Q9C9E1 | 43.4 | 53 | 27 | 1 | 16 | 68 | 70 | 119 | 6.8e-06 | 50.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38910.t1 | A0A396GHY8 | 100.0 | 54 | 0 | 0 | 15 | 68 | 74 | 127 | 9.8e-22 | 111.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049255 | MS.gene38910 | 0.802277 | 6.05E-49 | -1.69E-46 |
| MS.gene049341 | MS.gene38910 | 0.804287 | 2.32E-49 | -1.69E-46 |
| MS.gene050020 | MS.gene38910 | 0.810922 | 9.06E-51 | -1.69E-46 |
| MS.gene050807 | MS.gene38910 | 0.859395 | 4.26E-63 | -1.69E-46 |
| MS.gene050808 | MS.gene38910 | 0.82059 | 6.33E-53 | -1.69E-46 |
| MS.gene051243 | MS.gene38910 | 0.814372 | 1.59E-51 | -1.69E-46 |
| MS.gene05134 | MS.gene38910 | 0.803085 | 4.12E-49 | -1.69E-46 |
| MS.gene051789 | MS.gene38910 | 0.837193 | 6.01E-57 | -1.69E-46 |
| MS.gene052665 | MS.gene38910 | 0.803758 | 2.99E-49 | -1.69E-46 |
| MS.gene053074 | MS.gene38910 | 0.807276 | 5.47E-50 | -1.69E-46 |
| MS.gene053563 | MS.gene38910 | 0.802691 | 4.97E-49 | -1.69E-46 |
| MS.gene055608 | MS.gene38910 | 0.804389 | 2.21E-49 | -1.69E-46 |
| MS.gene056760 | MS.gene38910 | 0.805636 | 1.21E-49 | -1.69E-46 |
| MS.gene057057 | MS.gene38910 | 0.81188 | 5.61E-51 | -1.69E-46 |
| MS.gene057060 | MS.gene38910 | 0.800678 | 1.29E-48 | -1.69E-46 |
| MS.gene058055 | MS.gene38910 | 0.801087 | 1.06E-48 | -1.69E-46 |
| MS.gene059006 | MS.gene38910 | 0.80979 | 1.59E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38910.t1 | MTR_4g124700 | 55.769 | 52 | 21 | 1 | 16 | 67 | 72 | 121 | 1.02e-13 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38910.t1 | AT3G12955 | 50.000 | 58 | 25 | 2 | 14 | 67 | 80 | 137 | 6.35e-11 | 54.3 |
Find 10 sgRNAs with CRISPR-Local
Find 12 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTCTTTGATCATTTATTA+TGG | 0.230139 | 8.4:-50679766 | MS.gene38910:CDS |
| TTCTGAATCTTTAGAGGAAT+TGG | 0.285513 | 8.4:-50679714 | MS.gene38910:CDS |
| GTTTAGTTCTGAATCTTTAG+AGG | 0.292832 | 8.4:-50679720 | MS.gene38910:CDS |
| GATCAACAAGTCTAAGCAAA+AGG | 0.406159 | 8.4:-50679831 | MS.gene38910:CDS |
| TAGTGATAATGAAAATGTTT+TGG | 0.436689 | 8.4:-50679807 | MS.gene38910:CDS |
| AATTGTGAAACTATATTTGT+TGG | 0.453808 | 8.4:-50679917 | MS.gene38910:CDS |
| AAAGATTCAGAACTAAACTT+AGG | 0.498126 | 8.4:+50679725 | None:intergenic |
| CTATATTTGTTGGTAGCTCA+AGG | 0.523677 | 8.4:-50679907 | MS.gene38910:CDS |
| ATCAACAAGTCTAAGCAAAA+GGG | 0.536159 | 8.4:-50679830 | MS.gene38910:CDS |
| TTGTTGGTAGCTCAAGGAAA+CGG | 0.544116 | 8.4:-50679901 | MS.gene38910:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTGTGAAACTATATTTGT+TGG | - | chr8.4:50679699-50679718 | MS.gene38910:CDS | 20.0% |
| !! | TTCTCTTTGATCATTTATTA+TGG | - | chr8.4:50679850-50679869 | MS.gene38910:intron | 20.0% |
| !!! | TAGTGATAATGAAAATGTTT+TGG | - | chr8.4:50679809-50679828 | MS.gene38910:CDS | 20.0% |
| ! | AAAGATTCAGAACTAAACTT+AGG | + | chr8.4:50679894-50679913 | None:intergenic | 25.0% |
| ATCAACAAGTCTAAGCAAAA+GGG | - | chr8.4:50679786-50679805 | MS.gene38910:CDS | 30.0% | |
| ! | TTCTGAATCTTTAGAGGAAT+TGG | - | chr8.4:50679902-50679921 | MS.gene38910:CDS | 30.0% |
| !! | GTTTAGTTCTGAATCTTTAG+AGG | - | chr8.4:50679896-50679915 | MS.gene38910:intron | 30.0% |
| !!! | TGTTTTGGTTGTTAATTGTG+AGG | - | chr8.4:50679824-50679843 | MS.gene38910:CDS | 30.0% |
| GATCAACAAGTCTAAGCAAA+AGG | - | chr8.4:50679785-50679804 | MS.gene38910:CDS | 35.0% | |
| TTGATCAAAGCTTCAAGAAG+AGG | + | chr8.4:50679771-50679790 | None:intergenic | 35.0% | |
| !! | CTATATTTGTTGGTAGCTCA+AGG | - | chr8.4:50679709-50679728 | MS.gene38910:CDS | 35.0% |
| !! | TTGTTGGTAGCTCAAGGAAA+CGG | - | chr8.4:50679715-50679734 | MS.gene38910:CDS | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 50679694 | 50679944 | 50679694 | ID=MS.gene38910 |
| chr8.4 | mRNA | 50679694 | 50679944 | 50679694 | ID=MS.gene38910.t1;Parent=MS.gene38910 |
| chr8.4 | exon | 50679902 | 50679944 | 50679902 | ID=MS.gene38910.t1.exon1;Parent=MS.gene38910.t1 |
| chr8.4 | CDS | 50679902 | 50679944 | 50679902 | ID=cds.MS.gene38910.t1;Parent=MS.gene38910.t1 |
| chr8.4 | exon | 50679694 | 50679857 | 50679694 | ID=MS.gene38910.t1.exon2;Parent=MS.gene38910.t1 |
| chr8.4 | CDS | 50679694 | 50679857 | 50679694 | ID=cds.MS.gene38910.t1;Parent=MS.gene38910.t1 |
| Gene Sequence |
| Protein sequence |
