Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38942.t1 | RHN40794.1 | 100 | 207 | 0 | 0 | 1 | 207 | 46 | 252 | 1.10E-95 | 359.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38942.t1 | A0A072TQW7 | 100.0 | 207 | 0 | 0 | 1 | 207 | 1 | 207 | 8.2e-96 | 359.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049596 | MS.gene38942 | 0.861074 | 1.32E-63 | -1.69E-46 |
| MS.gene04972 | MS.gene38942 | 0.802017 | 6.85E-49 | -1.69E-46 |
| MS.gene049989 | MS.gene38942 | 0.808302 | 3.31E-50 | -1.69E-46 |
| MS.gene050297 | MS.gene38942 | 0.840289 | 9.51E-58 | -1.69E-46 |
| MS.gene05124 | MS.gene38942 | 0.849948 | 2.33E-60 | -1.69E-46 |
| MS.gene052143 | MS.gene38942 | 0.811909 | 5.53E-51 | -1.69E-46 |
| MS.gene052367 | MS.gene38942 | 0.82127 | 4.42E-53 | -1.69E-46 |
| MS.gene053173 | MS.gene38942 | 0.810963 | 8.87E-51 | -1.69E-46 |
| MS.gene053416 | MS.gene38942 | 0.807541 | 4.81E-50 | -1.69E-46 |
| MS.gene053417 | MS.gene38942 | 0.803144 | 4.01E-49 | -1.69E-46 |
| MS.gene054551 | MS.gene38942 | 0.804535 | 2.06E-49 | -1.69E-46 |
| MS.gene054643 | MS.gene38942 | 0.839646 | 1.40E-57 | -1.69E-46 |
| MS.gene055331 | MS.gene38942 | 0.80936 | 1.97E-50 | -1.69E-46 |
| MS.gene056266 | MS.gene38942 | -0.802411 | 5.68E-49 | -1.69E-46 |
| MS.gene058818 | MS.gene38942 | 0.821541 | 3.82E-53 | -1.69E-46 |
| MS.gene058844 | MS.gene38942 | 0.837914 | 3.92E-57 | -1.69E-46 |
| MS.gene06093 | MS.gene38942 | 0.811283 | 7.57E-51 | -1.69E-46 |
| MS.gene061260 | MS.gene38942 | 0.808127 | 3.61E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38942.t1 | MTR_8g445730 | 100.000 | 207 | 0 | 0 | 1 | 207 | 1 | 207 | 6.78e-152 | 419 |
| MS.gene38942.t1 | MTR_3g461540 | 37.500 | 192 | 113 | 3 | 23 | 207 | 41 | 232 | 1.70e-24 | 96.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38942.t1 | AT3G12870 | 72.139 | 201 | 52 | 1 | 10 | 206 | 5 | 205 | 1.38e-99 | 287 |
| MS.gene38942.t1 | AT5G56120 | 37.500 | 200 | 118 | 2 | 15 | 207 | 36 | 235 | 1.35e-27 | 104 |
Find 50 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTGCGGATTGAATCTATC+TGG | 0.255908 | 8.4:+49592576 | None:intergenic |
| GTTTGTCTCTTGCTGTCATA+AGG | 0.289653 | 8.4:-49592163 | MS.gene38942:CDS |
| CATGATTTGTGACAAGCTTT+AGG | 0.297093 | 8.4:+49592470 | None:intergenic |
| AAGGGTTATCTTGTGTAGTT+AGG | 0.302967 | 8.4:-49592144 | None:intergenic |
| GAGTTTGTGGGTTGGTGTTT+CGG | 0.319413 | 8.4:+49592661 | None:intergenic |
| CTTTGAGCCATTGCTTTAAG+AGG | 0.341158 | 8.4:+49592639 | None:intergenic |
| TGGGGCTTATATGGGCTTTG+AGG | 0.363451 | 8.4:-49592415 | MS.gene38942:CDS |
| GGGTTGGTGTTTCGGCAATT+TGG | 0.381774 | 8.4:+49592669 | None:intergenic |
| GAGTTGAAAGTAAAGAAGTT+AGG | 0.394819 | 8.4:-49592235 | MS.gene38942:CDS |
| CTAAAGCTTGTCACAAATCA+TGG | 0.398644 | 8.4:-49592469 | MS.gene38942:CDS |
| TTTGTCTCTTGCTGTCATAA+GGG | 0.402196 | 8.4:-49592162 | MS.gene38942:CDS |
| GAGTGATGTAGAGGCTCATA+TGG | 0.426429 | 8.4:-49592387 | MS.gene38942:CDS |
| GGAGCATGAGGGTAGTTGAA+TGG | 0.435925 | 8.4:+49592519 | None:intergenic |
| AAGTTAGGAAATGGTCTTCA+AGG | 0.444925 | 8.4:-49592220 | MS.gene38942:CDS |
| GTTGGAGTTTGATTTGTTGA+AGG | 0.447000 | 8.4:-49592291 | MS.gene38942:CDS |
| TGAGGGTAGTTGAATGGAAG+AGG | 0.447303 | 8.4:+49592525 | None:intergenic |
| AAAGTAAAGAAGTTAGGAAA+TGG | 0.454954 | 8.4:-49592229 | MS.gene38942:CDS |
| TTTGCGGATTGAATCTATCT+GGG | 0.463339 | 8.4:+49592577 | None:intergenic |
| TTGCTTTAAGAGGAGTTTGT+GGG | 0.465955 | 8.4:+49592649 | None:intergenic |
| AGGGTTATCTTGTGTAGTTA+GGG | 0.471643 | 8.4:-49592143 | None:intergenic |
| AAAGCATTGTGATTTCTTTG+CGG | 0.474720 | 8.4:+49592561 | None:intergenic |
| AAGAGTTGAAGAGGAGCATG+AGG | 0.477929 | 8.4:+49592507 | None:intergenic |
| GTGGAGGAATTGAAGAAGAA+AGG | 0.484418 | 8.4:-49592314 | MS.gene38942:CDS |
| GATGCTGTCGAGGGAGAAAG+AGG | 0.493198 | 8.4:-49592360 | MS.gene38942:CDS |
| AACAATAAAGAACAGAGTGA+TGG | 0.509344 | 8.4:+49592443 | None:intergenic |
| CAGTGTAAGATCTTCAATCA+TGG | 0.516363 | 8.4:-49592717 | MS.gene38942:CDS |
| AAAGAAGAAGAACTCATCAA+TGG | 0.532094 | 8.4:-49592620 | MS.gene38942:CDS |
| GTTTCGGCAATTTGGTGAAG+TGG | 0.538425 | 8.4:+49592677 | None:intergenic |
| GGAATTGAAGAAGAAAGGGT+TGG | 0.541831 | 8.4:-49592309 | MS.gene38942:CDS |
| TTTCGGCAATTTGGTGAAGT+GGG | 0.543957 | 8.4:+49592678 | None:intergenic |
| ATTGCTTTAAGAGGAGTTTG+TGG | 0.546009 | 8.4:+49592648 | None:intergenic |
| AGTTAGGAAATGGTCTTCAA+GGG | 0.556873 | 8.4:-49592219 | MS.gene38942:CDS |
| TAAGATCTTCAATCATGGAA+TGG | 0.557240 | 8.4:-49592712 | MS.gene38942:CDS |
| AGAGTTGAAGAGGAGCATGA+GGG | 0.559380 | 8.4:+49592508 | None:intergenic |
| AAGAGGATCGCGGATTGTTG+AGG | 0.566520 | 8.4:-49592343 | MS.gene38942:CDS |
| ACAATAAAGAACAGAGTGAT+GGG | 0.566612 | 8.4:+49592444 | None:intergenic |
| TAACAGAGGAAGAGTTGAAG+AGG | 0.573029 | 8.4:+49592498 | None:intergenic |
| AGGAAGTTGATGCTTTGAGA+AGG | 0.575406 | 8.4:-49592271 | MS.gene38942:CDS |
| GGAAGTTGATGCTTTGAGAA+GGG | 0.582663 | 8.4:-49592270 | MS.gene38942:CDS |
| TGGAGGAATTGAAGAAGAAA+GGG | 0.588646 | 8.4:-49592313 | MS.gene38942:CDS |
| ATATGGAGAAGATGCTGTCG+AGG | 0.600196 | 8.4:-49592370 | MS.gene38942:CDS |
| TTTAAGAGGAGTTTGTGGGT+TGG | 0.603717 | 8.4:+49592653 | None:intergenic |
| GAGGTATAAGAGTGATGTAG+AGG | 0.608995 | 8.4:-49592396 | MS.gene38942:CDS |
| TAAAGCTTGTCACAAATCAT+GGG | 0.609433 | 8.4:-49592468 | MS.gene38942:CDS |
| ACAAACTCCTCTTAAAGCAA+TGG | 0.614010 | 8.4:-49592646 | MS.gene38942:CDS |
| AGCTTTAGGTGTTGTAACAG+AGG | 0.622557 | 8.4:+49592484 | None:intergenic |
| TATGGAGAAGATGCTGTCGA+GGG | 0.624845 | 8.4:-49592369 | MS.gene38942:CDS |
| CGGATTGTTGAGGAAATGTG+TGG | 0.678295 | 8.4:-49592333 | MS.gene38942:CDS |
| TCGAGGGAGAAAGAGGATCG+CGG | 0.705803 | 8.4:-49592353 | MS.gene38942:CDS |
| ATTGTTGAGGAAATGTGTGG+AGG | 0.718156 | 8.4:-49592330 | MS.gene38942:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGTTCTTTATTGTTTTCATT+GGG | - | chr8.4:49592457-49592476 | MS.gene38942:CDS | 20.0% |
| ! | AAAGTAAAGAAGTTAGGAAA+TGG | - | chr8.4:49592662-49592681 | MS.gene38942:CDS | 25.0% |
| !! | CTGTTCTTTATTGTTTTCAT+TGG | - | chr8.4:49592456-49592475 | MS.gene38942:CDS | 25.0% |
| !! | GTTCTTTATTGTTTTCATTG+GGG | - | chr8.4:49592458-49592477 | MS.gene38942:CDS | 25.0% |
| AAAGAAGAAGAACTCATCAA+TGG | - | chr8.4:49592271-49592290 | MS.gene38942:CDS | 30.0% | |
| AACAATAAAGAACAGAGTGA+TGG | + | chr8.4:49592451-49592470 | None:intergenic | 30.0% | |
| ACAATAAAGAACAGAGTGAT+GGG | + | chr8.4:49592450-49592469 | None:intergenic | 30.0% | |
| GAGTTGAAAGTAAAGAAGTT+AGG | - | chr8.4:49592656-49592675 | MS.gene38942:CDS | 30.0% | |
| TAAAGCTTGTCACAAATCAT+GGG | - | chr8.4:49592423-49592442 | MS.gene38942:CDS | 30.0% | |
| TAAGATCTTCAATCATGGAA+TGG | - | chr8.4:49592179-49592198 | MS.gene38942:CDS | 30.0% | |
| !! | AAAGCATTGTGATTTCTTTG+CGG | + | chr8.4:49592333-49592352 | None:intergenic | 30.0% |
| !! | TTCTCTGTTTTTCTTCTCAA+TGG | - | chr8.4:49592702-49592721 | MS.gene38942:CDS | 30.0% |
| AAGTTAGGAAATGGTCTTCA+AGG | - | chr8.4:49592671-49592690 | MS.gene38942:CDS | 35.0% | |
| ACAAACTCCTCTTAAAGCAA+TGG | - | chr8.4:49592245-49592264 | MS.gene38942:CDS | 35.0% | |
| AGTTAGGAAATGGTCTTCAA+GGG | - | chr8.4:49592672-49592691 | MS.gene38942:CDS | 35.0% | |
| CAGTGTAAGATCTTCAATCA+TGG | - | chr8.4:49592174-49592193 | MS.gene38942:CDS | 35.0% | |
| CTAAAGCTTGTCACAAATCA+TGG | - | chr8.4:49592422-49592441 | MS.gene38942:CDS | 35.0% | |
| TGGAGGAATTGAAGAAGAAA+GGG | - | chr8.4:49592578-49592597 | MS.gene38942:CDS | 35.0% | |
| ! | CATGATTTGTGACAAGCTTT+AGG | + | chr8.4:49592424-49592443 | None:intergenic | 35.0% |
| ! | GTTTTCATTGGGGCTTATAT+GGG | - | chr8.4:49592468-49592487 | MS.gene38942:CDS | 35.0% |
| ! | TGTTTTCATTGGGGCTTATA+TGG | - | chr8.4:49592467-49592486 | MS.gene38942:CDS | 35.0% |
| ! | TTTGCGGATTGAATCTATCT+GGG | + | chr8.4:49592317-49592336 | None:intergenic | 35.0% |
| ! | TTTGTCTCTTGCTGTCATAA+GGG | - | chr8.4:49592729-49592748 | MS.gene38942:CDS | 35.0% |
| !! | ATTGCTTTAAGAGGAGTTTG+TGG | + | chr8.4:49592246-49592265 | None:intergenic | 35.0% |
| !! | GTTGGAGTTTGATTTGTTGA+AGG | - | chr8.4:49592600-49592619 | MS.gene38942:CDS | 35.0% |
| !! | TTGCTTTAAGAGGAGTTTGT+GGG | + | chr8.4:49592245-49592264 | None:intergenic | 35.0% |
| ATTGTTGAGGAAATGTGTGG+AGG | - | chr8.4:49592561-49592580 | MS.gene38942:CDS | 40.0% | |
| GAGGTATAAGAGTGATGTAG+AGG | - | chr8.4:49592495-49592514 | MS.gene38942:CDS | 40.0% | |
| GGAATTGAAGAAGAAAGGGT+TGG | - | chr8.4:49592582-49592601 | MS.gene38942:CDS | 40.0% | |
| GTGGAGGAATTGAAGAAGAA+AGG | - | chr8.4:49592577-49592596 | MS.gene38942:CDS | 40.0% | |
| TAACAGAGGAAGAGTTGAAG+AGG | + | chr8.4:49592396-49592415 | None:intergenic | 40.0% | |
| TTTAAGAGGAGTTTGTGGGT+TGG | + | chr8.4:49592241-49592260 | None:intergenic | 40.0% | |
| ! | CTTTGAGCCATTGCTTTAAG+AGG | + | chr8.4:49592255-49592274 | None:intergenic | 40.0% |
| ! | CTTTGCGGATTGAATCTATC+TGG | + | chr8.4:49592318-49592337 | None:intergenic | 40.0% |
| ! | GTTTGTCTCTTGCTGTCATA+AGG | - | chr8.4:49592728-49592747 | MS.gene38942:CDS | 40.0% |
| ! | TTTCGGCAATTTGGTGAAGT+GGG | + | chr8.4:49592216-49592235 | None:intergenic | 40.0% |
| !! | AGCTTTAGGTGTTGTAACAG+AGG | + | chr8.4:49592410-49592429 | None:intergenic | 40.0% |
| !! | AGGAAGTTGATGCTTTGAGA+AGG | - | chr8.4:49592620-49592639 | MS.gene38942:CDS | 40.0% |
| !! | GGAAGTTGATGCTTTGAGAA+GGG | - | chr8.4:49592621-49592640 | MS.gene38942:CDS | 40.0% |
| ATATGGAGAAGATGCTGTCG+AGG | - | chr8.4:49592521-49592540 | MS.gene38942:CDS | 45.0% | |
| CGGATTGTTGAGGAAATGTG+TGG | - | chr8.4:49592558-49592577 | MS.gene38942:CDS | 45.0% | |
| GAGTGATGTAGAGGCTCATA+TGG | - | chr8.4:49592504-49592523 | MS.gene38942:CDS | 45.0% | |
| TATGGAGAAGATGCTGTCGA+GGG | - | chr8.4:49592522-49592541 | MS.gene38942:CDS | 45.0% | |
| TGAGGGTAGTTGAATGGAAG+AGG | + | chr8.4:49592369-49592388 | None:intergenic | 45.0% | |
| ! | AAGAGTTGAAGAGGAGCATG+AGG | + | chr8.4:49592387-49592406 | None:intergenic | 45.0% |
| ! | AGAGTTGAAGAGGAGCATGA+GGG | + | chr8.4:49592386-49592405 | None:intergenic | 45.0% |
| ! | GTTTCGGCAATTTGGTGAAG+TGG | + | chr8.4:49592217-49592236 | None:intergenic | 45.0% |
| !! | GAGTTTGTGGGTTGGTGTTT+CGG | + | chr8.4:49592233-49592252 | None:intergenic | 45.0% |
| GGAGCATGAGGGTAGTTGAA+TGG | + | chr8.4:49592375-49592394 | None:intergenic | 50.0% | |
| TGGGGCTTATATGGGCTTTG+AGG | - | chr8.4:49592476-49592495 | MS.gene38942:CDS | 50.0% | |
| ! | AAGAGGATCGCGGATTGTTG+AGG | - | chr8.4:49592548-49592567 | MS.gene38942:CDS | 50.0% |
| !! | GGGTTGGTGTTTCGGCAATT+TGG | + | chr8.4:49592225-49592244 | None:intergenic | 50.0% |
| GATGCTGTCGAGGGAGAAAG+AGG | - | chr8.4:49592531-49592550 | MS.gene38942:CDS | 55.0% | |
| TCGAGGGAGAAAGAGGATCG+CGG | - | chr8.4:49592538-49592557 | MS.gene38942:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 49592145 | 49592768 | 49592145 | ID=MS.gene38942 |
| chr8.4 | mRNA | 49592145 | 49592768 | 49592145 | ID=MS.gene38942.t1;Parent=MS.gene38942 |
| chr8.4 | exon | 49592145 | 49592768 | 49592145 | ID=MS.gene38942.t1.exon1;Parent=MS.gene38942.t1 |
| chr8.4 | CDS | 49592145 | 49592768 | 49592145 | ID=cds.MS.gene38942.t1;Parent=MS.gene38942.t1 |
| Gene Sequence |
| Protein sequence |
