Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39152.t1 | RHN79300.1 | 48.7 | 189 | 87 | 1 | 7 | 185 | 27 | 215 | 7.00E-20 | 107.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39152.t1 | A0A072VJF9 | 48.7 | 189 | 87 | 1 | 7 | 185 | 62 | 250 | 5.0e-20 | 107.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049563 | MS.gene39152 | 0.807863 | 4.11E-50 | -1.69E-46 |
| MS.gene050139 | MS.gene39152 | 0.800701 | 1.27E-48 | -1.69E-46 |
| MS.gene051265 | MS.gene39152 | 0.800949 | 1.13E-48 | -1.69E-46 |
| MS.gene051626 | MS.gene39152 | 0.812355 | 4.42E-51 | -1.69E-46 |
| MS.gene052187 | MS.gene39152 | 0.821809 | 3.31E-53 | -1.69E-46 |
| MS.gene052698 | MS.gene39152 | 0.806079 | 9.79E-50 | -1.69E-46 |
| MS.gene053074 | MS.gene39152 | 0.845628 | 3.61E-59 | -1.69E-46 |
| MS.gene054185 | MS.gene39152 | 0.800746 | 1.25E-48 | -1.69E-46 |
| MS.gene05456 | MS.gene39152 | 0.80179 | 7.63E-49 | -1.69E-46 |
| MS.gene054596 | MS.gene39152 | 0.802732 | 4.88E-49 | -1.69E-46 |
| MS.gene055358 | MS.gene39152 | 0.81753 | 3.14E-52 | -1.69E-46 |
| MS.gene055359 | MS.gene39152 | 0.860028 | 2.75E-63 | -1.69E-46 |
| MS.gene058168 | MS.gene39152 | 0.860377 | 2.16E-63 | -1.69E-46 |
| MS.gene059006 | MS.gene39152 | 0.828854 | 7.13E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39152.t1 | MTR_1g054385 | 48.421 | 190 | 88 | 1 | 6 | 185 | 61 | 250 | 9.83e-50 | 161 |
| MS.gene39152.t1 | MTR_1g054380 | 41.176 | 204 | 70 | 6 | 1 | 169 | 1 | 189 | 3.13e-31 | 113 |
| MS.gene39152.t1 | MTR_5g048290 | 34.826 | 201 | 93 | 5 | 21 | 186 | 85 | 282 | 2.70e-20 | 85.9 |
| MS.gene39152.t1 | MTR_1g054370 | 32.843 | 204 | 96 | 5 | 21 | 186 | 90 | 290 | 2.07e-18 | 80.9 |
| MS.gene39152.t1 | MTR_1g054360 | 31.373 | 204 | 99 | 5 | 21 | 186 | 119 | 319 | 5.31e-11 | 60.5 |
| MS.gene39152.t1 | MTR_1g054365 | 26.404 | 178 | 107 | 3 | 19 | 186 | 108 | 271 | 9.40e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 34 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCAACTTGGTCAAGGTTTA+AGG | 0.142695 | 1.1:+41547869 | None:intergenic |
| CTTGTAAACAATTCCTTCTT+TGG | 0.221022 | 1.1:+41548011 | None:intergenic |
| CTTCGAAAAGAGTGGATTAA+TGG | 0.276341 | 1.1:-41548088 | MS.gene39152:CDS |
| GGAGCAAAAGGTTTGTGATT+TGG | 0.304158 | 1.1:-41548217 | MS.gene39152:CDS |
| CTTGGTCAAGGTTTAAGGTT+AGG | 0.388940 | 1.1:+41547874 | None:intergenic |
| ATGGCTACTCTACATCAATC+TGG | 0.390287 | 1.1:-41548356 | MS.gene39152:CDS |
| GCAAGCAACACCCGTTCAAT+TGG | 0.411385 | 1.1:-41548259 | MS.gene39152:CDS |
| TGCTCGTTGTATGGAGCAAA+AGG | 0.418843 | 1.1:-41548229 | MS.gene39152:CDS |
| GGAAAATTGCACACCAAAGA+AGG | 0.418990 | 1.1:-41548024 | MS.gene39152:CDS |
| GGTCTCGATTGCTCGTTGTA+TGG | 0.434380 | 1.1:-41548238 | MS.gene39152:CDS |
| CGCTGAAATTGAAGAATTGA+AGG | 0.453471 | 1.1:-41547962 | MS.gene39152:CDS |
| AGAAGGAATTGTTTACAAGT+TGG | 0.457133 | 1.1:-41548007 | MS.gene39152:CDS |
| GTGATTTGGAAATGAGCTGC+AGG | 0.481810 | 1.1:-41548203 | MS.gene39152:CDS |
| AGGAATTGTTTACAAGTTGG+AGG | 0.487776 | 1.1:-41548004 | MS.gene39152:CDS |
| AAAGGATCATGAGGCATATG+AGG | 0.490646 | 1.1:-41548313 | MS.gene39152:CDS |
| GAGTGGATTAATGGAAAGAG+AGG | 0.491512 | 1.1:-41548079 | MS.gene39152:CDS |
| CTGAATCTGAGAATAACATA+GGG | 0.510118 | 1.1:-41548140 | MS.gene39152:CDS |
| TCTGAATCTGAGAATAACAT+AGG | 0.510558 | 1.1:-41548141 | MS.gene39152:CDS |
| TCATGAGGCATATGAGGAGT+GGG | 0.527499 | 1.1:-41548307 | MS.gene39152:CDS |
| GCAATCGAGACCAATTGAAC+GGG | 0.527802 | 1.1:+41548249 | None:intergenic |
| GTAGAGTAGCCATTCTCAGC+TGG | 0.533250 | 1.1:+41548366 | None:intergenic |
| AAGACTACACCAGCTGAGAA+TGG | 0.552919 | 1.1:-41548375 | MS.gene39152:CDS |
| GCACAATGTTGGCTTTAATG+AGG | 0.553089 | 1.1:-41547923 | MS.gene39152:CDS |
| TGGCTACTCTACATCAATCT+GGG | 0.557094 | 1.1:-41548355 | MS.gene39152:CDS |
| ATTGTAGTGCAGCACAATGT+TGG | 0.569055 | 1.1:-41547934 | MS.gene39152:CDS |
| GAATTAATCTTCGAAAAGAG+TGG | 0.570774 | 1.1:-41548096 | MS.gene39152:CDS |
| ATCATGAGGCATATGAGGAG+TGG | 0.581486 | 1.1:-41548308 | MS.gene39152:CDS |
| AGAATGGATCCCAAGAGATG+TGG | 0.586826 | 1.1:-41548420 | MS.gene39152:CDS |
| CGGGTGTTGCTTGCAAAACA+CGG | 0.601763 | 1.1:+41548268 | None:intergenic |
| TATCACAGAAAAGGATCATG+AGG | 0.608121 | 1.1:-41548322 | MS.gene39152:CDS |
| AGCAATCGAGACCAATTGAA+CGG | 0.624783 | 1.1:+41548248 | None:intergenic |
| TGATTTGGAAATGAGCTGCA+GGG | 0.643574 | 1.1:-41548202 | MS.gene39152:CDS |
| CATGAGGCATATGAGGAGTG+GGG | 0.686751 | 1.1:-41548306 | MS.gene39152:CDS |
| TGAATCTGAGAATAACATAG+GGG | 0.713948 | 1.1:-41548139 | MS.gene39152:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGAAAACTCCAAGATTTT+AGG | - | chr1.1:41548251-41548270 | MS.gene39152:CDS | 25.0% |
| !! | TGACAATTTTATCACAGAAA+AGG | - | chr1.1:41547965-41547984 | MS.gene39152:CDS | 25.0% |
| AGAAGGAATTGTTTACAAGT+TGG | - | chr1.1:41548289-41548308 | MS.gene39152:CDS | 30.0% | |
| CTGAATCTGAGAATAACATA+GGG | - | chr1.1:41548156-41548175 | MS.gene39152:CDS | 30.0% | |
| CTTGTAAACAATTCCTTCTT+TGG | + | chr1.1:41548288-41548307 | None:intergenic | 30.0% | |
| GAATTAATCTTCGAAAAGAG+TGG | - | chr1.1:41548200-41548219 | MS.gene39152:CDS | 30.0% | |
| TCTGAATCTGAGAATAACAT+AGG | - | chr1.1:41548155-41548174 | MS.gene39152:CDS | 30.0% | |
| TGAATCTGAGAATAACATAG+GGG | - | chr1.1:41548157-41548176 | MS.gene39152:CDS | 30.0% | |
| ! | GTGCAATTTTCCTAAAATCT+TGG | + | chr1.1:41548264-41548283 | None:intergenic | 30.0% |
| CGCTGAAATTGAAGAATTGA+AGG | - | chr1.1:41548334-41548353 | MS.gene39152:CDS | 35.0% | |
| TATCACAGAAAAGGATCATG+AGG | - | chr1.1:41547974-41547993 | MS.gene39152:CDS | 35.0% | |
| ! | AGGAATTGTTTACAAGTTGG+AGG | - | chr1.1:41548292-41548311 | MS.gene39152:CDS | 35.0% |
| !! | CTTCGAAAAGAGTGGATTAA+TGG | - | chr1.1:41548208-41548227 | MS.gene39152:CDS | 35.0% |
| AAAGGATCATGAGGCATATG+AGG | - | chr1.1:41547983-41548002 | MS.gene39152:CDS | 40.0% | |
| AGCAATCGAGACCAATTGAA+CGG | + | chr1.1:41548051-41548070 | None:intergenic | 40.0% | |
| ATGGCTACTCTACATCAATC+TGG | - | chr1.1:41547940-41547959 | MS.gene39152:CDS | 40.0% | |
| ATTGTAGTGCAGCACAATGT+TGG | - | chr1.1:41548362-41548381 | MS.gene39152:CDS | 40.0% | |
| GGAAAATTGCACACCAAAGA+AGG | - | chr1.1:41548272-41548291 | MS.gene39152:CDS | 40.0% | |
| GGAGCAAAAGGTTTGTGATT+TGG | - | chr1.1:41548079-41548098 | MS.gene39152:CDS | 40.0% | |
| TGGCTACTCTACATCAATCT+GGG | - | chr1.1:41547941-41547960 | MS.gene39152:CDS | 40.0% | |
| ! | CTACGTTTTCCACATCTCTT+GGG | + | chr1.1:41547888-41547907 | None:intergenic | 40.0% |
| ! | TGATTTGGAAATGAGCTGCA+GGG | - | chr1.1:41548094-41548113 | MS.gene39152:CDS | 40.0% |
| !! | GAGTGGATTAATGGAAAGAG+AGG | - | chr1.1:41548217-41548236 | MS.gene39152:CDS | 40.0% |
| !! | GCACAATGTTGGCTTTAATG+AGG | - | chr1.1:41548373-41548392 | MS.gene39152:CDS | 40.0% |
| AAGACTACACCAGCTGAGAA+TGG | - | chr1.1:41547921-41547940 | MS.gene39152:CDS | 45.0% | |
| ATCATGAGGCATATGAGGAG+TGG | - | chr1.1:41547988-41548007 | MS.gene39152:CDS | 45.0% | |
| GCAATCGAGACCAATTGAAC+GGG | + | chr1.1:41548050-41548069 | None:intergenic | 45.0% | |
| TCATGAGGCATATGAGGAGT+GGG | - | chr1.1:41547989-41548008 | MS.gene39152:CDS | 45.0% | |
| TGCTCGTTGTATGGAGCAAA+AGG | - | chr1.1:41548067-41548086 | MS.gene39152:CDS | 45.0% | |
| ! | GCTACGTTTTCCACATCTCT+TGG | + | chr1.1:41547889-41547908 | None:intergenic | 45.0% |
| ! | GTGATTTGGAAATGAGCTGC+AGG | - | chr1.1:41548093-41548112 | MS.gene39152:CDS | 45.0% |
| CATGAGGCATATGAGGAGTG+GGG | - | chr1.1:41547990-41548009 | MS.gene39152:CDS | 50.0% | |
| GCAAGCAACACCCGTTCAAT+TGG | - | chr1.1:41548037-41548056 | MS.gene39152:CDS | 50.0% | |
| GGTCTCGATTGCTCGTTGTA+TGG | - | chr1.1:41548058-41548077 | MS.gene39152:CDS | 50.0% | |
| GTAGAGTAGCCATTCTCAGC+TGG | + | chr1.1:41547933-41547952 | None:intergenic | 50.0% | |
| !! | CGGGTGTTGCTTGCAAAACA+CGG | + | chr1.1:41548031-41548050 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 41547879 | 41548439 | 41547879 | ID=MS.gene39152 |
| chr1.1 | mRNA | 41547879 | 41548439 | 41547879 | ID=MS.gene39152.t1;Parent=MS.gene39152 |
| chr1.1 | exon | 41547879 | 41548439 | 41547879 | ID=MS.gene39152.t1.exon1;Parent=MS.gene39152.t1 |
| chr1.1 | CDS | 41547879 | 41548439 | 41547879 | ID=cds.MS.gene39152.t1;Parent=MS.gene39152.t1 |
| Gene Sequence |
| Protein sequence |
