AlfalfaGEDB | MS.gene41205

M. sativa cultivar XinJiangDaYe / MS.gene41205

KEGG: K11251
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF00125.27 PF16211.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41205.t1	RHN60978.1	96	149	3	1	1	149	1	146	7.30E-68	266.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41205.t1	Q2HU65	94.7	152	4	3	1	149	1	151	3.3e-69	262.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41205.t1	A0A396I838	96.0	149	3	1	1	149	1	146	5.2e-68	266.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052308	MS.gene41205	0.815897	7.30E-52	-1.69E-46
MS.gene053348	MS.gene41205	0.818058	2.39E-52	-1.69E-46
MS.gene054551	MS.gene41205	0.816638	4.99E-52	-1.69E-46
MS.gene054593	MS.gene41205	0.811098	8.30E-51	-1.69E-46
MS.gene054885	MS.gene41205	0.805758	1.14E-49	-1.69E-46
MS.gene055308	MS.gene41205	0.82467	7.12E-54	-1.69E-46
MS.gene055578	MS.gene41205	0.852909	3.39E-61	-1.69E-46
MS.gene055957	MS.gene41205	0.864895	8.75E-65	-1.69E-46
MS.gene056041	MS.gene41205	0.836929	7.01E-57	-1.69E-46
MS.gene05640	MS.gene41205	0.844012	9.84E-59	-1.69E-46
MS.gene057458	MS.gene41205	0.837019	6.65E-57	-1.69E-46
MS.gene058128	MS.gene41205	0.844231	8.59E-59	-1.69E-46
MS.gene058853	MS.gene41205	0.82596	3.53E-54	-1.69E-46
MS.gene059553	MS.gene41205	0.813049	3.12E-51	-1.69E-46
MS.gene06093	MS.gene41205	0.812547	4.01E-51	-1.69E-46
MS.gene061099	MS.gene41205	0.813324	2.71E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41205.t1	MTR_2g096570	94.737	152	4	3	1	149	1	151	1.02e-94	270
MS.gene41205.t1	MTR_4g063280	97.163	141	4	0	9	149	3	143	2.14e-93	267
MS.gene41205.t1	MTR_2g096610	88.591	149	14	2	1	149	1	146	1.08e-81	237
MS.gene41205.t1	MTR_4g071150	94.040	151	6	2	1	149	1	150	1.11e-81	237
MS.gene41205.t1	MTR_4g064005	93.960	149	6	1	1	149	1	146	2.26e-81	236
MS.gene41205.t1	MTR_4g063410	93.289	149	7	1	1	149	1	146	5.87e-81	235
MS.gene41205.t1	MTR_4g064967	93.960	149	6	1	1	149	1	146	1.40e-80	234
MS.gene41205.t1	MTR_8g086640	97.727	132	3	0	1	132	1	132	4.61e-76	223
MS.gene41205.t1	MTR_7g113160	82.090	134	22	2	10	143	13	144	5.24e-72	213
MS.gene41205.t1	MTR_7g108320	74.615	130	27	3	4	132	6	130	3.26e-60	182
MS.gene41205.t1	MTR_7g114060	73.846	130	30	1	3	132	2	127	4.02e-60	182
MS.gene41205.t1	MTR_7g114040	73.846	130	30	1	3	132	2	127	4.02e-60	182
MS.gene41205.t1	MTR_7g093910	72.441	127	32	1	4	130	5	128	2.82e-59	180
MS.gene41205.t1	MTR_2g082510	74.790	119	30	0	11	129	3	121	1.01e-58	179
MS.gene41205.t1	MTR_2g082220	73.950	119	31	0	11	129	3	121	1.19e-57	176
MS.gene41205.t1	MTR_6g005150	66.393	122	31	3	24	143	26	139	4.52e-48	152
MS.gene41205.t1	MTR_4g063300	80.000	105	15	1	45	149	10	108	1.01e-47	150
MS.gene41205.t1	MTR_7g114050	68.333	120	23	3	17	132	10	118	9.22e-46	145
MS.gene41205.t1	MTR_1g011750	59.292	113	44	2	21	132	23	134	9.57e-36	120
MS.gene41205.t1	MTR_1g011850	59.292	113	44	2	21	132	23	134	9.57e-36	120
MS.gene41205.t1	MTR_1g114050	59.633	109	42	2	21	128	20	127	7.91e-35	118
MS.gene41205.t1	MTR_1g071040	61.538	104	38	2	26	128	28	130	4.67e-34	116
MS.gene41205.t1	MTR_1g069770	52.174	92	43	1	45	135	9	100	1.53e-25	94.0
MS.gene41205.t1	MTR_7g010440	70.588	51	15	0	78	128	122	172	1.10e-16	75.1
MS.gene41205.t1	MTR_4g005910	74.419	43	11	0	87	129	4	46	1.83e-16	69.3
MS.gene41205.t1	MTR_4g048490	43.810	105	30	2	24	128	26	101	4.52e-16	69.7
MS.gene41205.t1	MTR_7g027610	41.818	110	38	3	20	128	10	94	4.35e-15	67.4
MS.gene41205.t1	MTR_0653s0030	53.704	54	25	0	56	109	44	97	1.31e-11	59.3
MS.gene41205.t1	MTR_2g096590	72.500	40	10	1	110	149	12	50	5.63e-11	55.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41205.t1	AT5G02560	84.106	151	22	1	1	149	1	151	1.47e-80	234
MS.gene41205.t1	AT5G02560	72.571	175	22	2	1	149	1	175	1.81e-75	223
MS.gene41205.t1	AT5G27670	74.834	151	33	3	1	149	1	148	9.19e-73	215
MS.gene41205.t1	AT5G59870	75.676	148	29	3	4	144	3	150	1.33e-71	212
MS.gene41205.t1	AT1G51060	76.667	120	28	0	11	130	3	122	3.89e-61	185
MS.gene41205.t1	AT4G27230	75.630	119	29	0	11	129	3	121	1.12e-59	181
MS.gene41205.t1	AT4G27230	75.630	119	29	0	11	129	3	121	1.12e-59	181
MS.gene41205.t1	AT3G20670	75.630	119	29	0	11	129	3	121	3.08e-59	180
MS.gene41205.t1	AT1G08880	71.318	129	34	1	7	132	2	130	1.43e-58	179
MS.gene41205.t1	AT5G54640	74.790	119	30	0	11	129	3	121	2.12e-58	178
MS.gene41205.t1	AT1G54690	72.519	131	30	3	3	132	5	130	5.55e-58	177
MS.gene41205.t1	AT2G38810	59.821	112	43	2	21	131	25	135	2.31e-36	122
MS.gene41205.t1	AT2G38810	59.821	112	43	2	21	131	25	135	2.31e-36	122
MS.gene41205.t1	AT2G38810	59.821	112	43	2	21	131	25	135	2.31e-36	122
MS.gene41205.t1	AT2G38810	59.821	112	43	2	21	131	25	135	2.31e-36	122
MS.gene41205.t1	AT1G52740	59.633	109	42	2	25	132	27	134	1.53e-34	117
MS.gene41205.t1	AT3G54560	58.716	109	43	2	25	132	29	136	2.45e-34	117
MS.gene41205.t1	AT3G54560	58.716	109	43	2	25	132	29	136	2.45e-34	117
MS.gene41205.t1	AT4G13570	47.826	92	46	2	32	122	28	118	6.91e-20	79.7
MS.gene41205.t1	AT4G13570	47.253	91	46	2	33	122	35	124	1.75e-19	79.0

Find 45 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCTCCTTTCTTTGTGGTTT+TGG	0.155065	4.1:-37070705	None:intergenic
TCCTCCTGCTCCTTTCTTTG+TGG	0.311932	4.1:-37070711	None:intergenic
GACTGAAAAGGCTGCTTCTA+AGG	0.313763	4.1:+37071078	MS.gene41205:CDS
TGCTGCTGAGGTTCTTGAAT+TGG	0.317581	4.1:+37070895	MS.gene41205:CDS
CTTGTCACCGACTTCTTCCT+TGG	0.333455	4.1:-37070749	None:intergenic
CGATTCTTCCCACTGGGAAC+TGG	0.359689	4.1:-37070790	None:intergenic
TCTTCTTAGGCAAGAGAATA+GGG	0.366947	4.1:-37071057	None:intergenic
AGAACTGCTGCTAGGTATAC+TGG	0.389575	4.1:-37070863	None:intergenic
AAAGGAGCAGGAGGAAGGAA+GGG	0.396180	4.1:+37070719	MS.gene41205:CDS
TACGCTCAGCGTGTTGGAAC+AGG	0.407073	4.1:+37070836	MS.gene41205:CDS
GTCTTCTTAGGCAAGAGAAT+AGG	0.408927	4.1:-37071058	None:intergenic
AAGAATCAGATCTAAGCCTT+TGG	0.432647	4.1:-37071133	None:intergenic
AAGAGAATAGGGTTAATGTT+TGG	0.433482	4.1:-37071046	None:intergenic
GGAGCAGGAGGAAGGAAGGG+AGG	0.436317	4.1:+37070722	MS.gene41205:CDS
GAAAGGAGCAGGAGGAAGGA+AGG	0.440519	4.1:+37070718	MS.gene41205:CDS
GGCATGTGTTGTTGGCTGTT+AGG	0.442957	4.1:+37070963	MS.gene41205:CDS
GCTGAGGTTCTTGAATTGGC+TGG	0.456012	4.1:+37070899	MS.gene41205:CDS
GCAGGAGGAAGGAAGGGAGG+AGG	0.468760	4.1:+37070725	MS.gene41205:CDS
TATTCCAAGGCATGTGTTGT+TGG	0.470522	4.1:+37070955	MS.gene41205:CDS
CAGTTCCCAGTGGGAAGAAT+CGG	0.476269	4.1:+37070791	MS.gene41205:CDS
AGCACCAAAACCACAAAGAA+AGG	0.482345	4.1:+37070701	MS.gene41205:CDS
TCTTGAATACCTTGCTGCTG+AGG	0.489347	4.1:+37070883	MS.gene41205:CDS
CAAAGAAAGGAGCAGGAGGA+AGG	0.496497	4.1:+37070714	MS.gene41205:CDS
GAGCTTGGTAAATTACTTGC+TGG	0.499780	4.1:+37070995	MS.gene41205:CDS
ACAGCCAACAACACATGCCT+TGG	0.515073	4.1:-37070959	None:intergenic
ATCGGTCGATACTTGAAGAA+AGG	0.519021	4.1:+37070809	MS.gene41205:CDS
AAAACCACAAAGAAAGGAGC+AGG	0.530917	4.1:+37070707	MS.gene41205:CDS
GGAAGATACGCTCAGCGTGT+TGG	0.531317	4.1:+37070830	MS.gene41205:CDS
GAAGGAAGGGAGGAGGACCA+AGG	0.532633	4.1:+37070732	MS.gene41205:CDS
CTTGCCTAAGAAGACTGAAA+AGG	0.532710	4.1:+37071066	MS.gene41205:CDS
TATCGACCGATTCTTCCCAC+TGG	0.544173	4.1:-37070797	None:intergenic
GTTAGGAACGATGAAGAGCT+TGG	0.552797	4.1:+37070980	MS.gene41205:CDS
TCCCACTGGGAACTGGAGAC+CGG	0.556836	4.1:-37070783	None:intergenic
ACCACAAAGAAAGGAGCAGG+AGG	0.568964	4.1:+37070710	MS.gene41205:CDS
GGTATTCAAGAACTGCTGCT+AGG	0.591966	4.1:-37070871	None:intergenic
GTGACAAGATCAATCAGAGC+CGG	0.601599	4.1:+37070764	MS.gene41205:CDS
AGGAGGACCAAGGAAGAAGT+CGG	0.610829	4.1:+37070742	MS.gene41205:CDS
GCCGGTCTCCAGTTCCCAGT+GGG	0.615696	4.1:+37070782	MS.gene41205:CDS
AGAAGAATAGAATTATTCCA+AGG	0.617592	4.1:+37070942	MS.gene41205:CDS
AAAAGCTGGAAAGTCTCCAA+AGG	0.624560	4.1:+37071117	MS.gene41205:CDS
ATCGACCGATTCTTCCCACT+GGG	0.626884	4.1:-37070796	None:intergenic
GCTGGTGTTACAATTGCTCA+CGG	0.638494	4.1:+37071013	MS.gene41205:CDS
ATTCAAGAACCTCAGCAGCA+AGG	0.646491	4.1:-37070892	None:intergenic
AGCCGGTCTCCAGTTCCCAG+TGG	0.691656	4.1:+37070781	MS.gene41205:CDS
GGTGTTACAATTGCTCACGG+TGG	0.811864	4.1:+37071016	MS.gene41205:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGAAGAATAGAATTATTCCA+AGG	+	chr4.1:37070942-37070961	MS.gene41205:CDS	25.0%
	AAGAGAATAGGGTTAATGTT+TGG	-	chr4.1:37071049-37071068	None:intergenic	30.0%
	TCTTCTTAGGCAAGAGAATA+GGG	-	chr4.1:37071060-37071079	None:intergenic	35.0%
	AAAACCACAAAGAAAGGAGC+AGG	+	chr4.1:37070707-37070726	MS.gene41205:CDS	40.0%
	AAAAGCTGGAAAGTCTCCAA+AGG	+	chr4.1:37071117-37071136	MS.gene41205:CDS	40.0%
	AGCACCAAAACCACAAAGAA+AGG	+	chr4.1:37070701-37070720	MS.gene41205:CDS	40.0%
	ATCGGTCGATACTTGAAGAA+AGG	+	chr4.1:37070809-37070828	MS.gene41205:CDS	40.0%
	CTTGCCTAAGAAGACTGAAA+AGG	+	chr4.1:37071066-37071085	MS.gene41205:CDS	40.0%
	GAGCTTGGTAAATTACTTGC+TGG	+	chr4.1:37070995-37071014	MS.gene41205:CDS	40.0%
	GTCTTCTTAGGCAAGAGAAT+AGG	-	chr4.1:37071061-37071080	None:intergenic	40.0%
	TATTCCAAGGCATGTGTTGT+TGG	+	chr4.1:37070955-37070974	MS.gene41205:CDS	40.0%
!	TGCTCCTTTCTTTGTGGTTT+TGG	-	chr4.1:37070708-37070727	None:intergenic	40.0%
!!!	GGAGACTTTCCAGCTTTTTT+GGG	-	chr4.1:37071115-37071134	None:intergenic	40.0%
!!!	TGGAGACTTTCCAGCTTTTT+TGG	-	chr4.1:37071116-37071135	None:intergenic	40.0%
	AGAACTGCTGCTAGGTATAC+TGG	-	chr4.1:37070866-37070885	None:intergenic	45.0%
	ATTCAAGAACCTCAGCAGCA+AGG	-	chr4.1:37070895-37070914	None:intergenic	45.0%
	CCTAAGTCTCCCAAAAAAGC+TGG	+	chr4.1:37071103-37071122	MS.gene41205:CDS	45.0%
	GACTGAAAAGGCTGCTTCTA+AGG	+	chr4.1:37071078-37071097	MS.gene41205:CDS	45.0%
	GGTATTCAAGAACTGCTGCT+AGG	-	chr4.1:37070874-37070893	None:intergenic	45.0%
	GTGACAAGATCAATCAGAGC+CGG	+	chr4.1:37070764-37070783	MS.gene41205:CDS	45.0%
	GTTAGGAACGATGAAGAGCT+TGG	+	chr4.1:37070980-37070999	MS.gene41205:CDS	45.0%
	TCTTGAATACCTTGCTGCTG+AGG	+	chr4.1:37070883-37070902	MS.gene41205:CDS	45.0%
	TGCTGCTGAGGTTCTTGAAT+TGG	+	chr4.1:37070895-37070914	MS.gene41205:CDS	45.0%
!	GCAGCCTTTTCAGTCTTCTT+AGG	-	chr4.1:37071073-37071092	None:intergenic	45.0%
!!	GCTGGTGTTACAATTGCTCA+CGG	+	chr4.1:37071013-37071032	MS.gene41205:CDS	45.0%
!!!	CCAGCTTTTTTGGGAGACTT+AGG	-	chr4.1:37071106-37071125	None:intergenic	45.0%
	AAAGGAGCAGGAGGAAGGAA+GGG	+	chr4.1:37070719-37070738	MS.gene41205:CDS	50.0%
	ACAGCCAACAACACATGCCT+TGG	-	chr4.1:37070962-37070981	None:intergenic	50.0%
	ACCACAAAGAAAGGAGCAGG+AGG	+	chr4.1:37070710-37070729	MS.gene41205:CDS	50.0%
	AGGAGGACCAAGGAAGAAGT+CGG	+	chr4.1:37070742-37070761	MS.gene41205:CDS	50.0%
	CAAAGAAAGGAGCAGGAGGA+AGG	+	chr4.1:37070714-37070733	MS.gene41205:CDS	50.0%
	CAGTTCCCAGTGGGAAGAAT+CGG	+	chr4.1:37070791-37070810	MS.gene41205:CDS	50.0%
	GCTGAGGTTCTTGAATTGGC+TGG	+	chr4.1:37070899-37070918	MS.gene41205:CDS	50.0%
	TCCTCCTGCTCCTTTCTTTG+TGG	-	chr4.1:37070714-37070733	None:intergenic	50.0%
!	ATCGACCGATTCTTCCCACT+GGG	-	chr4.1:37070799-37070818	None:intergenic	50.0%
!	GGCATGTGTTGTTGGCTGTT+AGG	+	chr4.1:37070963-37070982	MS.gene41205:CDS	50.0%
!	GGTGTTACAATTGCTCACGG+TGG	+	chr4.1:37071016-37071035	MS.gene41205:CDS	50.0%
!	TATCGACCGATTCTTCCCAC+TGG	-	chr4.1:37070800-37070819	None:intergenic	50.0%
!!	CTTGTCACCGACTTCTTCCT+TGG	-	chr4.1:37070752-37070771	None:intergenic	50.0%
	CGATTCTTCCCACTGGGAAC+TGG	-	chr4.1:37070793-37070812	None:intergenic	55.0%
	GAAAGGAGCAGGAGGAAGGA+AGG	+	chr4.1:37070718-37070737	MS.gene41205:CDS	55.0%
	GGAAGATACGCTCAGCGTGT+TGG	+	chr4.1:37070830-37070849	MS.gene41205:CDS	55.0%
	TACGCTCAGCGTGTTGGAAC+AGG	+	chr4.1:37070836-37070855	MS.gene41205:CDS	55.0%
	GAAGGAAGGGAGGAGGACCA+AGG	+	chr4.1:37070732-37070751	MS.gene41205:CDS	60.0%
	TCCCACTGGGAACTGGAGAC+CGG	-	chr4.1:37070786-37070805	None:intergenic	60.0%
	AGCCGGTCTCCAGTTCCCAG+TGG	+	chr4.1:37070781-37070800	MS.gene41205:CDS	65.0%
	GCAGGAGGAAGGAAGGGAGG+AGG	+	chr4.1:37070725-37070744	MS.gene41205:CDS	65.0%
	GCCGGTCTCCAGTTCCCAGT+GGG	+	chr4.1:37070782-37070801	MS.gene41205:CDS	65.0%
	GGAGCAGGAGGAAGGAAGGG+AGG	+	chr4.1:37070722-37070741	MS.gene41205:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	37070692	37071144	37070692	ID=MS.gene41205
chr4.1	mRNA	37070692	37071144	37070692	ID=MS.gene41205.t1;Parent=MS.gene41205
chr4.1	exon	37070692	37071144	37070692	ID=MS.gene41205.t1.exon1;Parent=MS.gene41205.t1
chr4.1	CDS	37070692	37071144	37070692	ID=cds.MS.gene41205.t1;Parent=MS.gene41205.t1

Gene Sequence

>MS.gene41205

ATGGACGCAAGCACCAAAACCACAAAGAAAGGAGCAGGAGGAAGGAAGGGAGGAGGACCAAGGAAGAAGTCGGTGACAAGATCAATCAGAGCCGGTCTCCAGTTCCCAGTGGGAAGAATCGGTCGATACTTGAAGAAAGGAAGATACGCTCAGCGTGTTGGAACAGGTGCACCAGTATACCTAGCAGCAGTTCTTGAATACCTTGCTGCTGAGGTTCTTGAATTGGCTGGAAATGCAGCACGTGATAACAAGAAGAATAGAATTATTCCAAGGCATGTGTTGTTGGCTGTTAGGAACGATGAAGAGCTTGGTAAATTACTTGCTGGTGTTACAATTGCTCACGGTGGTGTTCTTCCAAACATTAACCCTATTCTCTTGCCTAAGAAGACTGAAAAGGCTGCTTCTAAGGAACCTAAGTCTCCCAAAAAAGCTGGAAAGTCTCCAAAGGCTTAG

Protein sequence

>MS.gene41205.t1

MDASTKTTKKGAGGRKGGGPRKKSVTRSIRAGLQFPVGRIGRYLKKGRYAQRVGTGAPVYLAAVLEYLAAEVLELAGNAARDNKKNRIIPRHVLLAVRNDEELGKLLAGVTIAHGGVLPNINPILLPKKTEKAASKEPKSPKKAGKSPKA