AlfalfaGEDB | MS.gene43862

M. sativa cultivar XinJiangDaYe / MS.gene43862

KEGG: -
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43862.t1	XP_013465354.1	98.5	131	2	0	1	131	1	131	6.30E-68	266.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43862.t1	P0DKL1	37.5	96	52	3	6	101	4	91	1.6e-11	70.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43862.t1	G8A150	98.5	131	2	0	1	131	1	131	4.6e-68	266.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050277	MS.gene43862	0.81893	1.52E-52	-1.69E-46
MS.gene051633	MS.gene43862	0.81751	3.18E-52	-1.69E-46
MS.gene051903	MS.gene43862	0.818349	2.05E-52	-1.69E-46
MS.gene052302	MS.gene43862	0.800461	1.43E-48	-1.69E-46
MS.gene052309	MS.gene43862	-0.820413	6.95E-53	-1.69E-46
MS.gene052818	MS.gene43862	-0.821467	3.97E-53	-1.69E-46
MS.gene057079	MS.gene43862	0.83743	5.22E-57	-1.69E-46
MS.gene057234	MS.gene43862	0.805255	1.46E-49	-1.69E-46
MS.gene057452	MS.gene43862	-0.802178	6.35E-49	-1.69E-46
MS.gene057999	MS.gene43862	0.814346	1.61E-51	-1.69E-46
MS.gene058074	MS.gene43862	0.810328	1.22E-50	-1.69E-46
MS.gene058238	MS.gene43862	0.821881	3.19E-53	-1.69E-46
MS.gene058661	MS.gene43862	-0.801268	9.75E-49	-1.69E-46
MS.gene059872	MS.gene43862	-0.806516	7.92E-50	-1.69E-46
MS.gene060297	MS.gene43862	0.80706	6.08E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene43862	MS.gene20078	PPI
MS.gene43862	MS.gene22418	PPI
MS.gene43862	MS.gene007530	PPI
MS.gene43862	MS.gene007417	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43862.t1	MTR_2g095430	98.473	131	2	0	1	131	1	131	9.47e-95	269
MS.gene43862.t1	MTR_4g057800	56.190	105	42	1	16	116	5	109	4.28e-37	123
MS.gene43862.t1	MTR_4g057810	53.333	105	43	2	16	116	5	107	4.97e-35	117
MS.gene43862.t1	MTR_1g063340	41.837	98	52	2	18	112	2	97	2.51e-21	82.4
MS.gene43862.t1	MTR_1g063330	40.000	95	52	2	18	109	2	94	2.61e-19	77.0
MS.gene43862.t1	MTR_1g063310	43.158	95	49	2	21	115	5	94	3.20e-19	77.0
MS.gene43862.t1	MTR_1g063290	48.571	70	34	1	41	110	35	102	8.93e-19	75.9
MS.gene43862.t1	MTR_1g063300	47.297	74	37	1	37	110	31	102	9.95e-19	75.9
MS.gene43862.t1	MTR_7g101890	50.000	76	32	2	37	112	48	117	6.04e-18	74.3
MS.gene43862.t1	MTR_1g063260	40.506	79	45	1	34	112	30	106	1.49e-17	72.8
MS.gene43862.t1	MTR_1g063280	35.238	105	53	3	21	112	5	107	2.70e-16	69.7
MS.gene43862.t1	MTR_7g101920	43.678	87	44	3	40	124	30	113	7.69e-16	68.6
MS.gene43862.t1	MTR_4g072980	51.471	68	29	2	35	101	17	81	4.69e-15	66.2
MS.gene43862.t1	MTR_8g076040	39.175	97	52	3	5	100	2	92	8.95e-15	65.5
MS.gene43862.t1	MTR_3g117610	49.180	61	27	1	41	101	41	97	1.02e-14	65.9
MS.gene43862.t1	MTR_4g072190	48.333	60	27	1	42	101	33	88	6.40e-14	63.2
MS.gene43862.t1	MTR_5g021820	45.000	60	29	1	41	100	36	91	1.21e-13	62.8
MS.gene43862.t1	MTR_4g072820	43.662	71	35	2	32	101	7	73	1.71e-13	62.0
MS.gene43862.t1	MTR_4g072910	42.353	85	41	3	17	100	10	87	4.13e-13	61.2
MS.gene43862.t1	MTR_0388s0020	45.614	57	31	0	52	108	81	137	4.22e-13	62.4
MS.gene43862.t1	MTR_3g117640	50.847	59	25	2	44	101	33	88	6.32e-13	60.8
MS.gene43862.t1	MTR_7g118010	52.174	46	22	0	60	105	36	81	6.76e-13	60.8
MS.gene43862.t1	MTR_4g072310	40.964	83	42	3	27	108	10	86	1.42e-12	59.7
MS.gene43862.t1	MTR_8g461210	38.542	96	52	2	18	110	5	96	1.44e-12	60.5
MS.gene43862.t1	MTR_4g072300	42.424	66	33	2	44	108	25	86	1.79e-12	59.3
MS.gene43862.t1	MTR_1g024085	38.824	85	45	2	19	100	8	88	3.98e-12	59.7
MS.gene43862.t1	MTR_3g113310	45.614	57	27	1	44	100	41	93	6.16e-12	58.5
MS.gene43862.t1	MTR_3g092220	45.161	62	32	2	44	105	32	91	6.36e-12	58.5
MS.gene43862.t1	MTR_4g072880	45.588	68	30	3	34	100	17	78	7.69e-12	57.8
MS.gene43862.t1	MTR_4g025830	42.188	64	36	1	45	108	79	141	7.81e-12	59.3
MS.gene43862.t1	MTR_4g072240	36.364	88	49	3	22	108	5	86	8.52e-12	57.8
MS.gene43862.t1	MTR_4g072370	41.096	73	38	2	30	101	8	76	9.36e-12	57.4
MS.gene43862.t1	MTR_4g072220	42.424	66	33	2	44	108	25	86	1.05e-11	57.4
MS.gene43862.t1	MTR_4g072870	47.458	59	26	2	44	101	25	79	1.25e-11	57.0
MS.gene43862.t1	MTR_4g072890	44.444	63	30	2	44	105	25	83	1.49e-11	57.0
MS.gene43862.t1	MTR_8g026730	50.000	46	23	0	60	105	40	85	1.96e-11	57.0
MS.gene43862.t1	MTR_4g072250	50.000	58	23	3	44	100	25	77	2.04e-11	56.6
MS.gene43862.t1	MTR_4g072260	46.552	58	26	2	44	100	25	78	2.30e-11	56.6
MS.gene43862.t1	MTR_4g072860	47.059	68	29	3	34	100	17	78	2.91e-11	56.2
MS.gene43862.t1	MTR_4g072270	41.333	75	37	3	27	100	10	78	2.94e-11	56.2
MS.gene43862.t1	MTR_4g072330	45.763	59	27	2	44	101	22	76	2.95e-11	56.2
MS.gene43862.t1	MTR_4g072540	49.153	59	25	2	44	101	20	74	3.40e-11	55.8
MS.gene43862.t1	MTR_4g124750	35.955	89	50	2	16	101	37	121	4.84e-11	57.0
MS.gene43862.t1	MTR_4g072770	55.814	43	18	1	60	101	26	68	7.20e-11	55.1
MS.gene43862.t1	MTR_4g072480	47.458	59	26	2	44	101	24	78	7.70e-11	55.1
MS.gene43862.t1	MTR_2g043960	46.667	60	28	1	42	101	73	128	8.61e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43862.t1	AT2G28085	45.833	120	54	3	3	113	6	123	3.09e-27	98.2
MS.gene43862.t1	AT3G09870	54.667	75	32	1	36	110	36	108	6.25e-21	81.6
MS.gene43862.t1	AT1G19830	43.243	74	38	1	41	114	44	113	4.97e-15	66.6
MS.gene43862.t1	AT4G34780	43.210	81	44	2	44	123	27	106	2.11e-14	64.7
MS.gene43862.t1	AT4G34760	38.710	93	34	2	8	100	25	94	2.28e-14	64.7
MS.gene43862.t1	AT4G34810	40.964	83	47	2	33	114	37	118	9.96e-14	63.5
MS.gene43862.t1	AT3G61900	48.485	66	33	1	35	100	20	84	1.23e-13	63.5
MS.gene43862.t1	AT2G16580	38.710	93	35	2	8	100	25	95	1.47e-13	62.8
MS.gene43862.t1	AT3G43120	42.683	82	41	2	42	121	76	153	2.30e-13	63.5
MS.gene43862.t1	AT2G21220	45.902	61	29	1	41	101	36	92	3.16e-13	61.6
MS.gene43862.t1	AT4G38840	53.448	58	23	2	44	100	32	86	5.63e-13	60.8
MS.gene43862.t1	AT1G75580	48.333	60	27	1	42	101	41	96	8.19e-13	60.8
MS.gene43862.t1	AT4G34800	51.724	58	26	2	45	101	21	77	8.36e-13	60.5
MS.gene43862.t1	AT5G10990	43.023	86	45	2	18	103	14	95	1.41e-12	61.2
MS.gene43862.t1	AT4G38825	44.000	75	32	3	27	100	11	76	1.54e-12	59.7
MS.gene43862.t1	AT4G38860	46.774	62	28	2	41	101	36	93	2.48e-12	59.3
MS.gene43862.t1	AT5G20810	50.000	60	26	1	42	101	76	131	3.42e-12	60.5
MS.gene43862.t1	AT5G20810	48.438	64	29	1	38	101	72	131	3.66e-12	60.8
MS.gene43862.t1	AT2G46690	38.554	83	47	2	23	105	5	83	3.92e-12	59.3
MS.gene43862.t1	AT5G18030	45.333	75	30	4	27	100	11	75	3.96e-12	58.5
MS.gene43862.t1	AT4G38850	42.857	63	35	1	50	111	25	87	5.48e-12	58.2
MS.gene43862.t1	AT5G18020	35.870	92	50	4	25	115	8	91	7.20e-12	57.8
MS.gene43862.t1	AT1G19840	38.298	94	52	2	18	110	14	102	7.41e-12	59.3
MS.gene43862.t1	AT4G34770	40.000	85	48	2	17	100	7	89	8.14e-12	58.2
MS.gene43862.t1	AT2G18010	40.476	84	45	2	18	100	20	99	1.56e-11	57.4
MS.gene43862.t1	AT2G21210	47.368	57	27	2	45	100	28	82	1.76e-11	57.0
MS.gene43862.t1	AT5G18050	40.260	77	38	3	25	100	8	77	2.06e-11	56.6
MS.gene43862.t1	AT5G53590	44.444	63	34	1	44	105	45	107	2.31e-11	57.8
MS.gene43862.t1	AT5G18060	41.558	77	37	3	25	100	8	77	3.13e-11	56.2
MS.gene43862.t1	AT5G18080	40.260	77	38	3	25	100	8	77	3.48e-11	56.2
MS.gene43862.t1	AT2G21210	34.000	100	58	4	4	100	55	149	4.48e-11	57.4
MS.gene43862.t1	AT2G45210	35.526	76	42	2	39	108	67	141	7.75e-11	57.0

Find 20 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGATTAGTTAAACAACTTAA+TGG	0.258123	2.2:+6318597	None:intergenic
CTACAAAAGAAAATTGTAAT+AGG	0.272237	2.2:-6318726	MS.gene43862:CDS
TCCAACATTTGTAAGGCTAT+TGG	0.311111	2.2:-6318577	MS.gene43862:CDS
GAGAAAACAGAAGAAGAGTA+TGG	0.352500	2.2:-6318555	MS.gene43862:CDS
TCCAATAGCCTTACAAATGT+TGG	0.391382	2.2:+6318576	None:intergenic
TAACTAATCCAACATTTGTA+AGG	0.401955	2.2:-6318584	MS.gene43862:CDS
TACAAAAGAAAATTGTAATA+GGG	0.412265	2.2:-6318725	MS.gene43862:CDS
TGTCCTTCCTTTACATCCTC+TGG	0.426410	2.2:+6318675	None:intergenic
TTCACTAGGTTTGCAAGGAA+TGG	0.477249	2.2:+6318509	None:intergenic
AGGTTTGCAAGGAATGGTTA+AGG	0.513403	2.2:+6318515	None:intergenic
CTTTACATCCTCTGGCACAT+AGG	0.520298	2.2:+6318683	None:intergenic
TGAAGTTCACTAGGTTTGCA+AGG	0.531515	2.2:+6318504	None:intergenic
CTATGTGCCAGAGGATGTAA+AGG	0.546374	2.2:-6318682	MS.gene43862:CDS
AACATCTTGTGAAGTTCACT+AGG	0.579992	2.2:+6318495	None:intergenic
ACAAGATGTTGCAACAGCAA+TGG	0.597792	2.2:-6318482	MS.gene43862:CDS
TTGCTGTGATTGCTAAGGGT+AGG	0.602902	2.2:-6318650	MS.gene43862:CDS
GATGCTTCTCACACTGAACA+TGG	0.608352	2.2:+6318794	None:intergenic
TGTGATTGCTAAGGGTAGGA+AGG	0.616305	2.2:-6318646	MS.gene43862:CDS
GTGCCAGAGGATGTAAAGGA+AGG	0.617148	2.2:-6318678	MS.gene43862:CDS
AACATCAACCTATGTGCCAG+AGG	0.654930	2.2:-6318691	MS.gene43862:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TACAAAAGAAAATTGTAATA+GGG	-	chr2.2:6318498-6318517	MS.gene43862:CDS	15.0%
!!	CTACAAAAGAAAATTGTAAT+AGG	-	chr2.2:6318497-6318516	MS.gene43862:CDS	20.0%
!	TAACTAATCCAACATTTGTA+AGG	-	chr2.2:6318639-6318658	MS.gene43862:CDS	25.0%
!!!	GGATTAGTTAAACAACTTAA+TGG	+	chr2.2:6318629-6318648	None:intergenic	25.0%
!!	AGAAGCATCAACAAAAAGAA+TGG	-	chr2.2:6318443-6318462	MS.gene43862:CDS	30.0%
	AACATCTTGTGAAGTTCACT+AGG	+	chr2.2:6318731-6318750	None:intergenic	35.0%
	AGCATCAACAAAAAGAATGG+TGG	-	chr2.2:6318446-6318465	MS.gene43862:CDS	35.0%
	ATCAACAAAAAGAATGGTGG+AGG	-	chr2.2:6318449-6318468	MS.gene43862:CDS	35.0%
	GAGAAAACAGAAGAAGAGTA+TGG	-	chr2.2:6318668-6318687	MS.gene43862:CDS	35.0%
	TCCAACATTTGTAAGGCTAT+TGG	-	chr2.2:6318646-6318665	MS.gene43862:CDS	35.0%
!	TCCAATAGCCTTACAAATGT+TGG	+	chr2.2:6318650-6318669	None:intergenic	35.0%
!!	ACATTTTGCTGTGATTGCTA+AGG	-	chr2.2:6318568-6318587	MS.gene43862:CDS	35.0%
!!	CATTTTGCTGTGATTGCTAA+GGG	-	chr2.2:6318569-6318588	MS.gene43862:CDS	35.0%
!!!	GAGTATGGTTTTGATCATCA+AGG	-	chr2.2:6318683-6318702	MS.gene43862:CDS	35.0%
	ACAAGATGTTGCAACAGCAA+TGG	-	chr2.2:6318741-6318760	MS.gene43862:CDS	40.0%
	ACAGCAATGGCAAAAACAAG+AGG	-	chr2.2:6318754-6318773	MS.gene43862:CDS	40.0%
	AGGTTTGCAAGGAATGGTTA+AGG	+	chr2.2:6318711-6318730	None:intergenic	40.0%
	CAGCAATGGCAAAAACAAGA+GGG	-	chr2.2:6318755-6318774	MS.gene43862:CDS	40.0%
	TGAAGTTCACTAGGTTTGCA+AGG	+	chr2.2:6318722-6318741	None:intergenic	40.0%
	TTCACTAGGTTTGCAAGGAA+TGG	+	chr2.2:6318717-6318736	None:intergenic	40.0%
!	AACAAAAAGAATGGTGGAGG+TGG	-	chr2.2:6318452-6318471	MS.gene43862:CDS	40.0%
	AACATCAACCTATGTGCCAG+AGG	-	chr2.2:6318532-6318551	MS.gene43862:CDS	45.0%
	CAATGGCAAAAACAAGAGGG+AGG	-	chr2.2:6318758-6318777	MS.gene43862:CDS	45.0%
	CTATGTGCCAGAGGATGTAA+AGG	-	chr2.2:6318541-6318560	MS.gene43862:CDS	45.0%
	CTTTACATCCTCTGGCACAT+AGG	+	chr2.2:6318543-6318562	None:intergenic	45.0%
	GATGCTTCTCACACTGAACA+TGG	+	chr2.2:6318432-6318451	None:intergenic	45.0%
	TGTCCTTCCTTTACATCCTC+TGG	+	chr2.2:6318551-6318570	None:intergenic	45.0%
	TGTGATTGCTAAGGGTAGGA+AGG	-	chr2.2:6318577-6318596	MS.gene43862:CDS	45.0%
	TTGCTGTGATTGCTAAGGGT+AGG	-	chr2.2:6318573-6318592	MS.gene43862:CDS	45.0%
	GTGCCAGAGGATGTAAAGGA+AGG	-	chr2.2:6318545-6318564	MS.gene43862:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	6318425	6318820	6318425	ID=MS.gene43862
chr2.2	mRNA	6318425	6318820	6318425	ID=MS.gene43862.t1;Parent=MS.gene43862
chr2.2	exon	6318425	6318820	6318425	ID=MS.gene43862.t1.exon1;Parent=MS.gene43862.t1
chr2.2	CDS	6318425	6318820	6318425	ID=cds.MS.gene43862.t1;Parent=MS.gene43862.t1

Gene Sequence

>MS.gene43862

ATGGCCATGTTCAGTGTGAGAAGCATCAACAAAAAGAATGGTGGAGGTGGCATTGTGAAGCTCATTGAGAAGCTACAAAAGAAAATTGTAATAGGGAGAAACAAATCAACATCAACCTATGTGCCAGAGGATGTAAAGGAAGGACATTTTGCTGTGATTGCTAAGGGTAGGAAGGAAGAAGAAGCAAAGAGATTTGTGTTACCATTAAGTTGTTTAACTAATCCAACATTTGTAAGGCTATTGGAGAAAACAGAAGAAGAGTATGGTTTTGATCATCAAGGTGCCTTAACCATTCCTTGCAAACCTAGTGAACTTCACAAGATGTTGCAACAGCAATGGCAAAAACAAGAGGGAGGTGCTGAAATTAACTGCTACAACATGCAATTCCTCCAATAA

Protein sequence

>MS.gene43862.t1

MAMFSVRSINKKNGGGGIVKLIEKLQKKIVIGRNKSTSTYVPEDVKEGHFAVIAKGRKEEEAKRFVLPLSCLTNPTFVRLLEKTEEEYGFDHQGALTIPCKPSELHKMLQQQWQKQEGGAEINCYNMQFLQ