Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43985.t1 | XP_013468965.1 | 99.2 | 130 | 1 | 0 | 1 | 130 | 1 | 130 | 1.30E-60 | 242.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43985.t1 | O22969 | 73.5 | 113 | 30 | 0 | 12 | 124 | 14 | 126 | 7.6e-38 | 157.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43985.t1 | A0A072VMF5 | 99.2 | 130 | 1 | 0 | 1 | 130 | 1 | 130 | 9.2e-61 | 242.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050593 | MS.gene43985 | 0.812306 | 4.53E-51 | -1.69E-46 |
| MS.gene05105 | MS.gene43985 | 0.802794 | 4.74E-49 | -1.69E-46 |
| MS.gene051255 | MS.gene43985 | 0.806209 | 9.19E-50 | -1.69E-46 |
| MS.gene051360 | MS.gene43985 | 0.810292 | 1.24E-50 | -1.69E-46 |
| MS.gene051734 | MS.gene43985 | 0.805166 | 1.52E-49 | -1.69E-46 |
| MS.gene051858 | MS.gene43985 | 0.808946 | 2.41E-50 | -1.69E-46 |
| MS.gene052484 | MS.gene43985 | 0.800584 | 1.35E-48 | -1.69E-46 |
| MS.gene053449 | MS.gene43985 | 0.803792 | 2.94E-49 | -1.69E-46 |
| MS.gene055206 | MS.gene43985 | 0.801226 | 9.95E-49 | -1.69E-46 |
| MS.gene055722 | MS.gene43985 | 0.815607 | 8.48E-52 | -1.69E-46 |
| MS.gene056448 | MS.gene43985 | 0.80673 | 7.14E-50 | -1.69E-46 |
| MS.gene056511 | MS.gene43985 | 0.829717 | 4.40E-55 | -1.69E-46 |
| MS.gene057076 | MS.gene43985 | 0.807133 | 5.87E-50 | -1.69E-46 |
| MS.gene059711 | MS.gene43985 | 0.825226 | 5.26E-54 | -1.69E-46 |
| MS.gene059902 | MS.gene43985 | 0.805293 | 1.43E-49 | -1.69E-46 |
| MS.gene060804 | MS.gene43985 | 0.807287 | 5.44E-50 | -1.69E-46 |
| MS.gene060818 | MS.gene43985 | 0.800038 | 1.74E-48 | -1.69E-46 |
| MS.gene060837 | MS.gene43985 | 0.852476 | 4.50E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43985.t1 | MTR_1g080680 | 99.231 | 130 | 1 | 0 | 1 | 130 | 1 | 130 | 6.65e-92 | 261 |
| MS.gene43985.t1 | MTR_2g103500 | 82.000 | 100 | 18 | 0 | 13 | 112 | 19 | 118 | 2.77e-56 | 172 |
| MS.gene43985.t1 | MTR_4g011000 | 75.455 | 110 | 22 | 1 | 8 | 112 | 4 | 113 | 1.45e-54 | 167 |
| MS.gene43985.t1 | MTR_5g006970 | 57.143 | 91 | 39 | 0 | 20 | 110 | 41 | 131 | 1.75e-29 | 104 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43985.t1 | AT3G04620 | 89.109 | 101 | 11 | 0 | 14 | 114 | 33 | 133 | 3.59e-63 | 190 |
| MS.gene43985.t1 | AT2G34160 | 73.451 | 113 | 30 | 0 | 12 | 124 | 14 | 126 | 7.50e-56 | 171 |
| MS.gene43985.t1 | AT1G29250 | 77.228 | 101 | 23 | 0 | 12 | 112 | 14 | 114 | 4.35e-53 | 163 |
Find 26 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTGAATTCTGTTCTTCTTA+TGG | 0.143902 | 1.3:+57063831 | None:intergenic |
| CTGAATTCTGTTCTTCTTAT+GGG | 0.253699 | 1.3:+57063832 | None:intergenic |
| TCAGTAGCTGCTTCTGATTC+AGG | 0.262383 | 1.3:+57062542 | None:intergenic |
| TCATTTGCAGTTTCAGTTAC+AGG | 0.276939 | 1.3:+57063857 | None:intergenic |
| CCATAAGAAGAACAGAATTC+AGG | 0.338440 | 1.3:-57063831 | MS.gene43985:CDS |
| GTAAAAGAAAAGTGGTTTCT+TGG | 0.349846 | 1.3:+57063799 | None:intergenic |
| GAGGTTGAGCTTTCTGCTCT+TGG | 0.357617 | 1.3:-57063381 | MS.gene43985:CDS |
| GAGCTTTCTGCTCTTGGAAT+GGG | 0.372225 | 1.3:-57063375 | MS.gene43985:intron |
| GAAAAGTGGTTTCTTGGTGT+TGG | 0.373099 | 1.3:+57063805 | None:intergenic |
| TGAGCTTTCTGCTCTTGGAA+TGG | 0.445801 | 1.3:-57063376 | MS.gene43985:CDS |
| TTTGTAGATTGAAATTGTGC+TGG | 0.464511 | 1.3:-57062615 | MS.gene43985:intron |
| TCCATTGTTCTTCAAAATCT+CGG | 0.472867 | 1.3:+57062907 | None:intergenic |
| TTCAGTTACAGGTGCAGCTA+TGG | 0.492084 | 1.3:+57063868 | None:intergenic |
| TCAGGTGTGGTGGTGTCAGC+AGG | 0.492551 | 1.3:+57062560 | None:intergenic |
| CAAAGGTCGGCTTGTGCAGA+AGG | 0.493622 | 1.3:-57062742 | MS.gene43985:CDS |
| GACATCTACAGTTGGCATGA+AGG | 0.541089 | 1.3:-57062772 | MS.gene43985:CDS |
| TGAGAAGTTTGACAATATGA+TGG | 0.556772 | 1.3:-57062585 | MS.gene43985:CDS |
| TGCTTCTGATTCAGGTGTGG+TGG | 0.564894 | 1.3:+57062550 | None:intergenic |
| GAAGTATTGACATCTACAGT+TGG | 0.573871 | 1.3:-57062780 | MS.gene43985:CDS |
| TTGTAGATTGAAATTGTGCT+GGG | 0.576481 | 1.3:-57062614 | MS.gene43985:intron |
| AGATTGACGTAAAAGAAAAG+TGG | 0.580806 | 1.3:+57063791 | None:intergenic |
| AGCTGCTTCTGATTCAGGTG+TGG | 0.603045 | 1.3:+57062547 | None:intergenic |
| TGAAGGATGAGAACAAAGGT+CGG | 0.640401 | 1.3:-57062755 | MS.gene43985:CDS |
| TCGGCTTGTGCAGAAGGCAA+AGG | 0.641693 | 1.3:-57062736 | MS.gene43985:intron |
| ATACATTCAGCAGCACAATG+AGG | 0.717661 | 1.3:-57063400 | MS.gene43985:CDS |
| GGCATGAAGGATGAGAACAA+AGG | 0.737049 | 1.3:-57062759 | MS.gene43985:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTATTTACATTACTTTGA+TGG | - | chr1.3:57062887-57062906 | MS.gene43985:intron | 15.0% |
| !! | GCTATATAAAGAAAAAAAAA+TGG | - | chr1.3:57062933-57062952 | MS.gene43985:CDS | 15.0% |
| !! | TTAAAACAATGTAATGTATA+AGG | - | chr1.3:57063288-57063307 | MS.gene43985:intron | 15.0% |
| !! | AAAAAGAAAAACAGAAAATG+AGG | - | chr1.3:57063314-57063333 | MS.gene43985:intron | 20.0% |
| !! | AGAAGAATCCAATTAATAAT+CGG | + | chr1.3:57063109-57063128 | None:intergenic | 20.0% |
| !! | ATAAAGAAAAAAAAATGGCT+AGG | - | chr1.3:57062938-57062957 | MS.gene43985:intron | 20.0% |
| !! | CAAATTAAAGACAAAAAGAA+GGG | + | chr1.3:57063081-57063100 | None:intergenic | 20.0% |
| !! | CTAATCAATTAATGAATGTT+TGG | - | chr1.3:57063593-57063612 | MS.gene43985:intron | 20.0% |
| !!! | AACTTTCTTAATTTTAAGCA+CGG | - | chr1.3:57062721-57062740 | MS.gene43985:intron | 20.0% |
| !!! | ATTTTAATGTTAGTTGTTTC+TGG | - | chr1.3:57063186-57063205 | MS.gene43985:intron | 20.0% |
| !!! | TCAAGACATTATTTTTACTA+TGG | - | chr1.3:57063234-57063253 | MS.gene43985:intron | 20.0% |
| ! | ACAAAAATGTTGACATGAAT+TGG | - | chr1.3:57063445-57063464 | MS.gene43985:intron | 25.0% |
| ! | GCAAATTAAAGACAAAAAGA+AGG | + | chr1.3:57063082-57063101 | None:intergenic | 25.0% |
| ! | TAGAGTAAGAATATGAAACT+AGG | + | chr1.3:57063404-57063423 | None:intergenic | 25.0% |
| ! | TATAACTCTCATTCATATGA+TGG | - | chr1.3:57063560-57063579 | MS.gene43985:intron | 25.0% |
| ! | TTGAAATTGTGATCAACTAA+TGG | - | chr1.3:57062911-57062930 | MS.gene43985:CDS | 25.0% |
| !! | ACAAAAAGAAGGGAAATTTT+AGG | + | chr1.3:57063071-57063090 | None:intergenic | 25.0% |
| !! | ATGTAATGTCACTTGATAAT+CGG | - | chr1.3:57062646-57062665 | MS.gene43985:intron | 25.0% |
| !! | TACTTTTGTTCAATTGATAG+AGG | + | chr1.3:57063365-57063384 | None:intergenic | 25.0% |
| !! | TGAATTCTTTTCATTTTCGT+CGG | + | chr1.3:57063701-57063720 | None:intergenic | 25.0% |
| !!! | TATTTTTACTATGGTGTGTA+AGG | - | chr1.3:57063243-57063262 | MS.gene43985:intron | 25.0% |
| AATTGATAGAGGATATCTGT+TGG | + | chr1.3:57063354-57063373 | None:intergenic | 30.0% | |
| AGATTGACGTAAAAGAAAAG+TGG | + | chr1.3:57062616-57062635 | None:intergenic | 30.0% | |
| CAAAATACCAAGAAAAGTCT+AGG | + | chr1.3:57063773-57063792 | None:intergenic | 30.0% | |
| CTGAATTCTGTTCTTCTTAT+GGG | + | chr1.3:57062575-57062594 | None:intergenic | 30.0% | |
| GTAAAAGAAAAGTGGTTTCT+TGG | + | chr1.3:57062608-57062627 | None:intergenic | 30.0% | |
| TCCATTGTTCTTCAAAATCT+CGG | + | chr1.3:57063500-57063519 | None:intergenic | 30.0% | |
| TGAGAAGTTTGACAATATGA+TGG | - | chr1.3:57063819-57063838 | MS.gene43985:CDS | 30.0% | |
| TTGCACAACCGATTATTAAT+TGG | - | chr1.3:57063098-57063117 | MS.gene43985:intron | 30.0% | |
| TTGTAGATTGAAATTGTGCT+GGG | - | chr1.3:57063790-57063809 | MS.gene43985:CDS | 30.0% | |
| TTTGTAGATTGAAATTGTGC+TGG | - | chr1.3:57063789-57063808 | MS.gene43985:CDS | 30.0% | |
| ! | TGTAATGTCACTTGATAATC+GGG | - | chr1.3:57062647-57062666 | MS.gene43985:intron | 30.0% |
| AACACACACATTCATGTAGT+CGG | + | chr1.3:57062825-57062844 | None:intergenic | 35.0% | |
| CAAGTGACATTACATTACCT+TGG | + | chr1.3:57062641-57062660 | None:intergenic | 35.0% | |
| CCATAAGAAGAACAGAATTC+AGG | - | chr1.3:57062573-57062592 | MS.gene43985:CDS | 35.0% | |
| CCTGAATTCTGTTCTTCTTA+TGG | + | chr1.3:57062576-57062595 | None:intergenic | 35.0% | |
| TCATTTGCAGTTTCAGTTAC+AGG | + | chr1.3:57062550-57062569 | None:intergenic | 35.0% | |
| TGAATGTATCTCTGCATCAA+AGG | + | chr1.3:57062993-57063012 | None:intergenic | 35.0% | |
| ! | TCTTAAGCCTAGACTTTTCT+TGG | - | chr1.3:57063763-57063782 | MS.gene43985:intron | 35.0% |
| !! | GAAGTATTGACATCTACAGT+TGG | - | chr1.3:57063624-57063643 | MS.gene43985:intron | 35.0% |
| ACTTGCAACTGAGAAGAGTA+AGG | - | chr1.3:57063519-57063538 | MS.gene43985:intron | 40.0% | |
| ATACATTCAGCAGCACAATG+AGG | - | chr1.3:57063004-57063023 | MS.gene43985:intron | 40.0% | |
| TGAAGGATGAGAACAAAGGT+CGG | - | chr1.3:57063649-57063668 | MS.gene43985:intron | 40.0% | |
| ! | ACTTGATAATCGGGATACCT+TGG | - | chr1.3:57062656-57062675 | MS.gene43985:intron | 40.0% |
| ! | GAAAAGTGGTTTCTTGGTGT+TGG | + | chr1.3:57062602-57062621 | None:intergenic | 40.0% |
| !! | GCCGAGATTTTGAAGAACAA+TGG | - | chr1.3:57063496-57063515 | MS.gene43985:intron | 40.0% |
| CATTCATGTAGTCGGTGCAT+TGG | + | chr1.3:57062817-57062836 | None:intergenic | 45.0% | |
| GACATCTACAGTTGGCATGA+AGG | - | chr1.3:57063632-57063651 | MS.gene43985:intron | 45.0% | |
| GAGCTTTCTGCTCTTGGAAT+GGG | - | chr1.3:57063029-57063048 | MS.gene43985:intron | 45.0% | |
| GGCATGAAGGATGAGAACAA+AGG | - | chr1.3:57063645-57063664 | MS.gene43985:intron | 45.0% | |
| GTAGTGACAGCATGAATCCA+AGG | + | chr1.3:57062676-57062695 | None:intergenic | 45.0% | |
| TCAGTAGCTGCTTCTGATTC+AGG | + | chr1.3:57063865-57063884 | None:intergenic | 45.0% | |
| TGAGCTTTCTGCTCTTGGAA+TGG | - | chr1.3:57063028-57063047 | MS.gene43985:intron | 45.0% | |
| TTCAGTTACAGGTGCAGCTA+TGG | + | chr1.3:57062539-57062558 | None:intergenic | 45.0% | |
| ! | CTTTTACGTCAATCTCGCCA+AGG | - | chr1.3:57062621-57062640 | MS.gene43985:intron | 45.0% |
| AGCTGCTTCTGATTCAGGTG+TGG | + | chr1.3:57063860-57063879 | None:intergenic | 50.0% | |
| GAGGTTGAGCTTTCTGCTCT+TGG | - | chr1.3:57063023-57063042 | MS.gene43985:intron | 50.0% | |
| TGCTTCTGATTCAGGTGTGG+TGG | + | chr1.3:57063857-57063876 | None:intergenic | 50.0% | |
| ! | TCGGCTTGTGCAGAAGGCAA+AGG | - | chr1.3:57063668-57063687 | MS.gene43985:intron | 55.0% |
| !! | CAAAGGTCGGCTTGTGCAGA+AGG | - | chr1.3:57063662-57063681 | MS.gene43985:intron | 55.0% |
| ! | TCAGGTGTGGTGGTGTCAGC+AGG | + | chr1.3:57063847-57063866 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 57062529 | 57063897 | 57062529 | ID=MS.gene43985 |
| chr1.3 | mRNA | 57062529 | 57063897 | 57062529 | ID=MS.gene43985.t1;Parent=MS.gene43985 |
| chr1.3 | exon | 57063784 | 57063897 | 57063784 | ID=MS.gene43985.t1.exon1;Parent=MS.gene43985.t1 |
| chr1.3 | CDS | 57063784 | 57063897 | 57063784 | ID=cds.MS.gene43985.t1;Parent=MS.gene43985.t1 |
| chr1.3 | exon | 57063376 | 57063424 | 57063376 | ID=MS.gene43985.t1.exon2;Parent=MS.gene43985.t1 |
| chr1.3 | CDS | 57063376 | 57063424 | 57063376 | ID=cds.MS.gene43985.t1;Parent=MS.gene43985.t1 |
| chr1.3 | exon | 57062891 | 57062953 | 57062891 | ID=MS.gene43985.t1.exon3;Parent=MS.gene43985.t1 |
| chr1.3 | CDS | 57062891 | 57062953 | 57062891 | ID=cds.MS.gene43985.t1;Parent=MS.gene43985.t1 |
| chr1.3 | exon | 57062737 | 57062801 | 57062737 | ID=MS.gene43985.t1.exon4;Parent=MS.gene43985.t1 |
| chr1.3 | CDS | 57062737 | 57062801 | 57062737 | ID=cds.MS.gene43985.t1;Parent=MS.gene43985.t1 |
| chr1.3 | exon | 57062529 | 57062630 | 57062529 | ID=MS.gene43985.t1.exon5;Parent=MS.gene43985.t1 |
| chr1.3 | CDS | 57062529 | 57062630 | 57062529 | ID=cds.MS.gene43985.t1;Parent=MS.gene43985.t1 |
| Gene Sequence |
| Protein sequence |
