AlfalfaGEDB | MS.gene46281

M. sativa cultivar XinJiangDaYe / MS.gene46281

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF01428.19 PF01754.19

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46281.t1	RHN61069.1	98.4	187	3	0	1	187	1	187	2.20E-90	341.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46281.t1	A2YEZ6	62.0	187	55	4	1	187	1	171	5.0e-59	228.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46281.t1	A0A396I620	98.4	187	3	0	1	187	1	187	1.6e-90	341.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049703	MS.gene46281	0.825702	4.06E-54	-1.69E-46
MS.gene051063	MS.gene46281	-0.828929	6.84E-55	-1.69E-46
MS.gene051746	MS.gene46281	-0.84293	1.91E-58	-1.69E-46
MS.gene051748	MS.gene46281	-0.836086	1.15E-56	-1.69E-46
MS.gene052856	MS.gene46281	-0.811274	7.60E-51	-1.69E-46
MS.gene056069	MS.gene46281	0.82906	6.36E-55	-1.69E-46
MS.gene056601	MS.gene46281	-0.823783	1.15E-53	-1.69E-46
MS.gene058906	MS.gene46281	-0.808243	3.41E-50	-1.69E-46
MS.gene06001	MS.gene46281	-0.823215	1.56E-53	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene050335	MS.gene46281	PPI
MS.gene46281	MS.gene24724	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46281.t1	MTR_2g098160	74.332	187	33	2	1	187	1	172	3.62e-97	279
MS.gene46281.t1	MTR_4g065570	85.350	157	3	1	31	187	1	137	2.01e-92	266
MS.gene46281.t1	MTR_7g092400	57.297	185	63	3	3	187	92	260	2.00e-70	214
MS.gene46281.t1	MTR_0249s0070	55.676	185	63	3	3	187	4	169	3.58e-68	205
MS.gene46281.t1	MTR_0249s0070	55.676	185	63	3	3	187	12	177	4.17e-68	206
MS.gene46281.t1	MTR_7g104320	40.761	184	91	5	4	187	3	168	3.78e-40	134
MS.gene46281.t1	MTR_4g053440	39.674	184	95	4	4	187	3	170	2.85e-39	132
MS.gene46281.t1	MTR_2g086190	39.884	173	72	4	15	187	10	150	1.06e-37	127
MS.gene46281.t1	MTR_1g060380	42.703	185	74	6	4	187	3	156	8.57e-35	120
MS.gene46281.t1	MTR_8g036980	65.574	61	21	0	127	187	91	151	1.58e-26	99.0
MS.gene46281.t1	MTR_3g028010	59.016	61	25	0	127	187	103	163	1.61e-23	91.7
MS.gene46281.t1	MTR_1g100773	30.814	172	82	5	15	185	3	138	1.05e-22	89.0
MS.gene46281.t1	MTR_7g114920	30.000	170	77	4	15	184	3	130	4.48e-22	87.0
MS.gene46281.t1	MTR_3g025570	72.727	44	12	0	122	165	4	47	2.74e-19	79.3
MS.gene46281.t1	MTR_2g054650	30.409	171	70	4	15	185	4	125	1.86e-17	75.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46281.t1	AT1G51200	59.893	187	61	4	1	187	1	173	2.00e-64	196
MS.gene46281.t1	AT1G51200	59.893	187	61	4	1	187	1	173	2.00e-64	196
MS.gene46281.t1	AT1G51200	59.893	187	61	4	1	187	1	173	2.00e-64	196
MS.gene46281.t1	AT1G51200	59.893	187	61	4	1	187	1	173	2.00e-64	196
MS.gene46281.t1	AT2G36320	41.573	178	76	3	12	187	10	161	1.73e-42	140
MS.gene46281.t1	AT2G27580	39.306	173	86	3	15	187	10	163	2.39e-39	132
MS.gene46281.t1	AT2G27580	39.306	173	86	3	15	187	10	163	2.39e-39	132
MS.gene46281.t1	AT4G12040	36.872	179	96	3	11	187	12	175	7.30e-39	131
MS.gene46281.t1	AT4G12040	36.872	179	96	3	11	187	12	175	7.30e-39	131
MS.gene46281.t1	AT1G12440	38.068	176	89	1	12	187	13	168	8.98e-38	128
MS.gene46281.t1	AT1G12440	38.068	176	89	1	12	187	13	168	8.98e-38	128
MS.gene46281.t1	AT1G12440	38.068	176	89	1	12	187	13	168	8.98e-38	128
MS.gene46281.t1	AT4G22820	36.994	173	94	2	15	187	19	176	8.47e-37	126
MS.gene46281.t1	AT4G22820	36.994	173	94	2	15	187	19	176	8.47e-37	126
MS.gene46281.t1	AT3G52800	42.021	188	85	5	4	187	3	170	3.63e-36	124
MS.gene46281.t1	AT3G52800	42.021	188	85	5	4	187	3	170	3.63e-36	124
MS.gene46281.t1	AT3G12630	36.747	166	68	5	23	187	31	160	1.00e-27	102
MS.gene46281.t1	AT4G14225	32.948	173	64	2	15	187	5	125	8.66e-26	96.3
MS.gene46281.t1	AT4G25380	28.736	174	67	3	18	187	10	130	3.03e-22	87.4

Find 50 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGCTTAGAGATAGTTTC+AGG	0.302295	4.4:+40792569	None:intergenic
AGCTTCTCAGCCTTGACAAC+CGG	0.320370	4.4:+40792338	None:intergenic
TTCGGACCATCCTTCGGCTT+TGG	0.323240	4.4:+40792512	None:intergenic
ATGCACAATATAGGACCTTC+AGG	0.357857	4.4:+40792833	None:intergenic
GTTTCAGCATCATGTCTTTG+TGG	0.367335	4.4:+40792756	None:intergenic
CTTGACAACCGGGTTTGCTT+TGG	0.380130	4.4:+40792349	None:intergenic
AGTTTCAGGTTCCACAGAAA+TGG	0.392851	4.4:+40792583	None:intergenic
TTGCAGTTGCTGCAACGTTT+CGG	0.398329	4.4:+40792494	None:intergenic
TCCCTTGGAAATATTATGAA+TGG	0.415286	4.4:-40792707	MS.gene46281:CDS
CAAAGACATGATGCTGAAAC+AGG	0.424095	4.4:-40792753	MS.gene46281:CDS
AATAAGCGAGTTGGTTTGAC+AGG	0.426782	4.4:-40792473	MS.gene46281:CDS
CAACGTTTCGGACCATCCTT+CGG	0.445047	4.4:+40792506	None:intergenic
AACCGGGTTTGCTTTGGCTA+TGG	0.447808	4.4:+40792355	None:intergenic
CAGTTGTTGATGCACAATAT+AGG	0.453708	4.4:+40792824	None:intergenic
TTAGGTTCCACGGAAATGGC+TGG	0.467021	4.4:+40792629	None:intergenic
GCACTTAGAGCACATGTTCA+TGG	0.487125	4.4:+40792778	None:intergenic
CCCTTGGAAATATTATGAAT+GGG	0.495769	4.4:-40792706	MS.gene46281:CDS
CAGAAGTGCGGTAATCGAAC+GGG	0.502252	4.4:+40792387	None:intergenic
GCTTCTCAGCCTTGACAACC+GGG	0.508433	4.4:+40792339	None:intergenic
AAGGGATGATGCTGCAAGAT+GGG	0.510002	4.4:+40792724	None:intergenic
ACGCTGGCAGCAGTAACAAC+AGG	0.516439	4.4:+40792659	None:intergenic
CCCATTCATAATATTTCCAA+GGG	0.520390	4.4:+40792706	None:intergenic
ACCCATTCATAATATTTCCA+AGG	0.526039	4.4:+40792705	None:intergenic
CAAGGGATGATGCTGCAAGA+TGG	0.532070	4.4:+40792723	None:intergenic
TCAGGTTCCACAGAAATGGA+TGG	0.538028	4.4:+40792587	None:intergenic
AGCAACTGCAATAAGCGAGT+TGG	0.543723	4.4:-40792482	MS.gene46281:CDS
AGCGATGTACAGCACAGAAA+AGG	0.543830	4.4:+40792429	None:intergenic
CATCTTGCAGCATCATCCCT+TGG	0.544464	4.4:-40792722	MS.gene46281:CDS
CAAAGCAAACCCGGTTGTCA+AGG	0.546068	4.4:-40792348	MS.gene46281:CDS
TGCCATAGCCAAAGCAAACC+CGG	0.547092	4.4:-40792357	MS.gene46281:CDS
TTTATTTGGCTCAGGTTCAG+AGG	0.556901	4.4:-40792546	MS.gene46281:CDS
GCAGAAGTGCGGTAATCGAA+CGG	0.558885	4.4:+40792386	None:intergenic
TAGGTTCCACGGAAATGGCT+GGG	0.559679	4.4:+40792630	None:intergenic
TGCACAATATAGGACCTTCA+GGG	0.559940	4.4:+40792834	None:intergenic
CAGGTTCCACAGAAATGGAT+GGG	0.560627	4.4:+40792588	None:intergenic
CATGATGCTGAAACAGGAGC+AGG	0.593551	4.4:-40792747	MS.gene46281:CDS
ACAGGGTTTAATTGTCGATG+TGG	0.597602	4.4:-40792455	MS.gene46281:CDS
GGTGATCCAAAGCCGAAGGA+TGG	0.601061	4.4:-40792518	MS.gene46281:CDS
CGATATCCCATCCATTTCTG+TGG	0.604057	4.4:-40792594	MS.gene46281:CDS
AATGGATGGGATATCGACAC+TGG	0.619918	4.4:+40792601	None:intergenic
TGGCATCTCGTGCAGAAGTG+CGG	0.628982	4.4:+40792375	None:intergenic
GAGTGGTGATCCAAAGCCGA+AGG	0.630939	4.4:-40792522	MS.gene46281:CDS
GGATATCCCAGCCATTTCCG+TGG	0.638474	4.4:-40792636	MS.gene46281:CDS
GCACAATATAGGACCTTCAG+GGG	0.644318	4.4:+40792835	None:intergenic
ATAAGCGAGTTGGTTTGACA+GGG	0.651214	4.4:-40792472	MS.gene46281:CDS
AATGGCTGGGATATCCACGC+TGG	0.652769	4.4:+40792643	None:intergenic
GGCTCAGGTTCAGAGGAGAG+TGG	0.659270	4.4:-40792539	MS.gene46281:CDS
TGTTGTTACTGCTGCCAGCG+TGG	0.668169	4.4:-40792657	MS.gene46281:CDS
ATATTGTGCATCAACAACTG+TGG	0.675543	4.4:-40792821	MS.gene46281:CDS
CACAATATAGGACCTTCAGG+GGG	0.733275	4.4:+40792836	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATCTCTAAGCCTTTTTTATT+TGG	-	chr4.4:40792631-40792650	MS.gene46281:CDS	25.0%
	ACCCATTCATAATATTTCCA+AGG	+	chr4.4:40792489-40792508	None:intergenic	30.0%
	CCCATTCATAATATTTCCAA+GGG	+	chr4.4:40792488-40792507	None:intergenic	30.0%
	CCCTTGGAAATATTATGAAT+GGG	-	chr4.4:40792485-40792504	MS.gene46281:CDS	30.0%
	TCCCTTGGAAATATTATGAA+TGG	-	chr4.4:40792484-40792503	MS.gene46281:CDS	30.0%
!	ATCAACAACTGTGGATTTTT+CGG	-	chr4.4:40792379-40792398	MS.gene46281:CDS	30.0%
	AAAGGCTTAGAGATAGTTTC+AGG	+	chr4.4:40792625-40792644	None:intergenic	35.0%
	ATATTGTGCATCAACAACTG+TGG	-	chr4.4:40792370-40792389	MS.gene46281:CDS	35.0%
	CAGTTGTTGATGCACAATAT+AGG	+	chr4.4:40792370-40792389	None:intergenic	35.0%
	GAACCTGAGCCAAATAAAAA+AGG	+	chr4.4:40792643-40792662	None:intergenic	35.0%
!!!	AAGCCTTTTTTATTTGGCTC+AGG	-	chr4.4:40792637-40792656	MS.gene46281:CDS	35.0%
	ACAGGGTTTAATTGTCGATG+TGG	-	chr4.4:40792736-40792755	MS.gene46281:CDS	40.0%
	ATGCACAATATAGGACCTTC+AGG	+	chr4.4:40792361-40792380	None:intergenic	40.0%
	CAAAGACATGATGCTGAAAC+AGG	-	chr4.4:40792438-40792457	MS.gene46281:CDS	40.0%
	GTTTCAGCATCATGTCTTTG+TGG	+	chr4.4:40792438-40792457	None:intergenic	40.0%
	TGCACAATATAGGACCTTCA+GGG	+	chr4.4:40792360-40792379	None:intergenic	40.0%
!	ATATCGACACTGGCAGTTTT+AGG	+	chr4.4:40792583-40792602	None:intergenic	40.0%
!	TTTATTTGGCTCAGGTTCAG+AGG	-	chr4.4:40792645-40792664	MS.gene46281:CDS	40.0%
!!	AATAAGCGAGTTGGTTTGAC+AGG	-	chr4.4:40792718-40792737	MS.gene46281:CDS	40.0%
!!	AGTTTCAGGTTCCACAGAAA+TGG	+	chr4.4:40792611-40792630	None:intergenic	40.0%
!!	ATAAGCGAGTTGGTTTGACA+GGG	-	chr4.4:40792719-40792738	MS.gene46281:CDS	40.0%
!!!	AGTTTTAGGTTCCACGGAAA+TGG	+	chr4.4:40792569-40792588	None:intergenic	40.0%
	AAGGGATGATGCTGCAAGAT+GGG	+	chr4.4:40792470-40792489	None:intergenic	45.0%
	AATGGATGGGATATCGACAC+TGG	+	chr4.4:40792593-40792612	None:intergenic	45.0%
	AGCAACTGCAATAAGCGAGT+TGG	-	chr4.4:40792709-40792728	MS.gene46281:CDS	45.0%
	AGCGATGTACAGCACAGAAA+AGG	+	chr4.4:40792765-40792784	None:intergenic	45.0%
	CACAATATAGGACCTTCAGG+GGG	+	chr4.4:40792358-40792377	None:intergenic	45.0%
	CAGGTTCCACAGAAATGGAT+GGG	+	chr4.4:40792606-40792625	None:intergenic	45.0%
	CGATATCCCATCCATTTCTG+TGG	-	chr4.4:40792597-40792616	MS.gene46281:CDS	45.0%
	GCACAATATAGGACCTTCAG+GGG	+	chr4.4:40792359-40792378	None:intergenic	45.0%
	GCACTTAGAGCACATGTTCA+TGG	+	chr4.4:40792416-40792435	None:intergenic	45.0%
	TCAGGTTCCACAGAAATGGA+TGG	+	chr4.4:40792607-40792626	None:intergenic	45.0%
	TTGCAGTTGCTGCAACGTTT+CGG	+	chr4.4:40792700-40792719	None:intergenic	45.0%
!!!	ACTGGCAGTTTTAGGTTCCA+CGG	+	chr4.4:40792575-40792594	None:intergenic	45.0%
	AGCTTCTCAGCCTTGACAAC+CGG	+	chr4.4:40792856-40792875	None:intergenic	50.0%
	CAAAGCAAACCCGGTTGTCA+AGG	-	chr4.4:40792843-40792862	MS.gene46281:CDS	50.0%
	CAACGTTTCGGACCATCCTT+CGG	+	chr4.4:40792688-40792707	None:intergenic	50.0%
	CAAGGGATGATGCTGCAAGA+TGG	+	chr4.4:40792471-40792490	None:intergenic	50.0%
	CATCTTGCAGCATCATCCCT+TGG	-	chr4.4:40792469-40792488	MS.gene46281:CDS	50.0%
	CATGATGCTGAAACAGGAGC+AGG	-	chr4.4:40792444-40792463	MS.gene46281:CDS	50.0%
	TAGGTTCCACGGAAATGGCT+GGG	+	chr4.4:40792564-40792583	None:intergenic	50.0%
	TGCCATAGCCAAAGCAAACC+CGG	-	chr4.4:40792834-40792853	MS.gene46281:CDS	50.0%
	TTAGGTTCCACGGAAATGGC+TGG	+	chr4.4:40792565-40792584	None:intergenic	50.0%
!	AACCGGGTTTGCTTTGGCTA+TGG	+	chr4.4:40792839-40792858	None:intergenic	50.0%
!	CAGAAGTGCGGTAATCGAAC+GGG	+	chr4.4:40792807-40792826	None:intergenic	50.0%
!	CTTGACAACCGGGTTTGCTT+TGG	+	chr4.4:40792845-40792864	None:intergenic	50.0%
!	GCAGAAGTGCGGTAATCGAA+CGG	+	chr4.4:40792808-40792827	None:intergenic	50.0%
	AATGGCTGGGATATCCACGC+TGG	+	chr4.4:40792551-40792570	None:intergenic	55.0%
	ACGCTGGCAGCAGTAACAAC+AGG	+	chr4.4:40792535-40792554	None:intergenic	55.0%
	GAATGTCAAGCTCCCCCTGA+AGG	-	chr4.4:40792343-40792362	MS.gene46281:CDS	55.0%
	GAGTGGTGATCCAAAGCCGA+AGG	-	chr4.4:40792669-40792688	MS.gene46281:CDS	55.0%
	GCTTCTCAGCCTTGACAACC+GGG	+	chr4.4:40792855-40792874	None:intergenic	55.0%
	GGATATCCCAGCCATTTCCG+TGG	-	chr4.4:40792555-40792574	MS.gene46281:CDS	55.0%
	GGTGATCCAAAGCCGAAGGA+TGG	-	chr4.4:40792673-40792692	MS.gene46281:CDS	55.0%
	TGGCATCTCGTGCAGAAGTG+CGG	+	chr4.4:40792819-40792838	None:intergenic	55.0%
	TGTTGTTACTGCTGCCAGCG+TGG	-	chr4.4:40792534-40792553	MS.gene46281:CDS	55.0%
	TTCGGACCATCCTTCGGCTT+TGG	+	chr4.4:40792682-40792701	None:intergenic	55.0%
	GGCTCAGGTTCAGAGGAGAG+TGG	-	chr4.4:40792652-40792671	MS.gene46281:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	40792325	40792888	40792325	ID=MS.gene46281
chr4.4	mRNA	40792325	40792888	40792325	ID=MS.gene46281.t1;Parent=MS.gene46281
chr4.4	exon	40792325	40792888	40792325	ID=MS.gene46281.t1.exon1;Parent=MS.gene46281.t1
chr4.4	CDS	40792325	40792888	40792325	ID=cds.MS.gene46281.t1;Parent=MS.gene46281.t1

Gene Sequence

>MS.gene46281

ATGGAACATGAAAAGACTGAATGTCAAGCTCCCCCTGAAGGTCCTATATTGTGCATCAACAACTGTGGATTTTTCGGAAGTGCAGCTACCATGAACATGTGCTCTAAGTGCCACAAAGACATGATGCTGAAACAGGAGCAGGCCCATCTTGCAGCATCATCCCTTGGAAATATTATGAATGGGTCTACAAGCAACACTGAAAAAGAACCTGTTGTTACTGCTGCCAGCGTGGATATCCCAGCCATTTCCGTGGAACCTAAAACTGCCAGTGTCGATATCCCATCCATTTCTGTGGAACCTGAAACTATCTCTAAGCCTTTTTTATTTGGCTCAGGTTCAGAGGAGAGTGGTGATCCAAAGCCGAAGGATGGTCCGAAACGTTGCAGCAACTGCAATAAGCGAGTTGGTTTGACAGGGTTTAATTGTCGATGTGGCAACCTTTTCTGTGCTGTACATCGCTACTCAGACAAGCATGATTGCCCGTTCGATTACCGCACTTCTGCACGAGATGCCATAGCCAAAGCAAACCCGGTTGTCAAGGCTGAGAAGCTTGATAAGATCTGA

Protein sequence

>MS.gene46281.t1

MEHEKTECQAPPEGPILCINNCGFFGSAATMNMCSKCHKDMMLKQEQAHLAASSLGNIMNGSTSNTEKEPVVTAASVDIPAISVEPKTASVDIPSISVEPETISKPFLFGSGSEESGDPKPKDGPKRCSNCNKRVGLTGFNCRCGNLFCAVHRYSDKHDCPFDYRTSARDAIAKANPVVKAEKLDKI