AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056601


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056601.t1 MTR_4g068040 100.000 69 0 0 1 69 1 69 3.43e-45 139
MS.gene056601.t1 MTR_2g018680 94.203 69 4 0 1 69 1 69 3.96e-42 131
MS.gene056601.t1 MTR_8g079485 94.203 69 4 0 1 69 1 69 4.72e-42 131
MS.gene056601.t1 MTR_4g068040 100.000 63 0 0 1 63 1 63 1.25e-40 127
MS.gene056601.t1 MTR_2g018680 95.312 64 3 0 1 64 1 64 5.36e-39 123
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056601.t1 AT3G59540 92.754 69 5 0 1 69 1 69 2.24e-42 132
MS.gene056601.t1 AT2G43460 92.754 69 5 0 1 69 1 69 2.24e-42 132

Find 11 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGCAAATCAATGAGATTA+AGG 0.290766 4.3:+36811688 MS.gene056601:CDS
CTTCAACTTATCAGCTTTCT+CGG 0.350966 4.3:-36811831 None:intergenic
TTTGCTTCTCTTGATCTTCA+CGG 0.377542 4.3:-36811747 None:intergenic
CAAAGATGTTGTCAAGTTCA+AGG 0.412153 4.3:+36811766 MS.gene056601:CDS
ACAAAGTGTGTACAAGTACT+TGG 0.506752 4.3:-36811798 None:intergenic
TTAATCTCATTGATTTGCTT+AGG 0.531275 4.3:-36811686 None:intergenic
AGGACTTCCTCCTCACTGCT+AGG 0.557805 4.3:+36811708 MS.gene056601:CDS
CCTCCTCACTGCTAGGAGGA+AGG 0.566472 4.3:+36811715 MS.gene056601:CDS
CCTTCCTCCTAGCAGTGAGG+AGG 0.657731 4.3:-36811715 None:intergenic
ACTTCCTCCTCACTGCTAGG+AGG 0.666318 4.3:+36811711 MS.gene056601:CDS
CATCCTTCCTCCTAGCAGTG+AGG 0.669946 4.3:-36811718 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTATATTTTTTTTTATT+TGG + chr4.3:36811587-36811606 MS.gene056601:intron 0.0%
!!! TATTTTTTTTTATTTGGATT+TGG + chr4.3:36811593-36811612 MS.gene056601:intron 10.0%
!! TCTATAAAGTAAATAATAAG+AGG + chr4.3:36812043-36812062 MS.gene056601:intron 15.0%
!!! GTGTAATAATTCTTTAAAAA+TGG + chr4.3:36812018-36812037 MS.gene056601:intron 15.0%
!! AAAACATTGAAAATCAAGAT+AGG - chr4.3:36811427-36811446 None:intergenic 20.0%
!! AAAGAATTATTACACCTTTA+AGG - chr4.3:36812013-36812032 None:intergenic 20.0%
!! ACATTTCAATAAACACAAAA+AGG - chr4.3:36811664-36811683 None:intergenic 20.0%
!! ATTATACATTGATCTGAATT+AGG + chr4.3:36811490-36811509 MS.gene056601:intron 20.0%
!! TCAGATTTATGAATATGAAA+TGG - chr4.3:36812211-36812230 None:intergenic 20.0%
!! TGAGATTTATGAATAAGAAA+TGG - chr4.3:36812116-36812135 None:intergenic 20.0%
!! TTATACATTGATCTGAATTA+GGG + chr4.3:36811491-36811510 MS.gene056601:intron 20.0%
!!! CTTGATTTTCAATGTTTTTA+GGG + chr4.3:36811429-36811448 MS.gene056601:intron 20.0%
!!! TAGTTGATAAAGTTGATAAA+GGG + chr4.3:36811543-36811562 MS.gene056601:intron 20.0%
!!! TAGTTTTTGAGAATTATTGT+TGG + chr4.3:36811872-36811891 MS.gene056601:intron 20.0%
!!! TCTTGATTTTCAATGTTTTT+AGG + chr4.3:36811428-36811447 MS.gene056601:intron 20.0%
!!! TTTTTATTTGGATTTGGATT+TGG + chr4.3:36811599-36811618 MS.gene056601:intron 20.0%
! AACAAGTGAAATTGAATCAA+TGG + chr4.3:36811459-36811478 MS.gene056601:intron 25.0%
! ATAATAAGAGGATTGTGTAA+TGG + chr4.3:36812055-36812074 MS.gene056601:intron 25.0%
! ATAATTCTCAAAAACTAACC+TGG - chr4.3:36811869-36811888 None:intergenic 25.0%
! CAACAGAAATAAACAATCTA+TGG - chr4.3:36811973-36811992 None:intergenic 25.0%
! TAAGCAAATCAATGAGATTA+AGG + chr4.3:36811688-36811707 MS.gene056601:CDS 25.0%
! TAATTCTCAAAAACTAACCT+GGG - chr4.3:36811868-36811887 None:intergenic 25.0%
! TTATGAATATGAAATGGAAG+AGG - chr4.3:36812205-36812224 None:intergenic 25.0%
!! AGATGTAGAAATTTGAGTTT+AGG + chr4.3:36812285-36812304 MS.gene056601:intron 25.0%
!! TTATTGTTGGTTGTATGTTA+TGG + chr4.3:36811885-36811904 MS.gene056601:intron 25.0%
!!! CAATTCACAGTTTTTTTTGT+AGG + chr4.3:36811377-36811396 MS.gene056601:intron 25.0%
!!! CAGTTTTTTTTGTAGGAAAT+TGG + chr4.3:36811384-36811403 MS.gene056601:intron 25.0%
!!! CATAGATTGTTTATTTCTGT+TGG + chr4.3:36811971-36811990 MS.gene056601:intron 25.0%
!!! GTAGTTGATAAAGTTGATAA+AGG + chr4.3:36811542-36811561 MS.gene056601:intron 25.0%
!!! TGGTTTTTGATTTTCAAACT+TGG + chr4.3:36811520-36811539 MS.gene056601:intron 25.0%
!!! TGTTATTTTTCTCTCATTCT+AGG + chr4.3:36812239-36812258 MS.gene056601:intron 25.0%
!!! TTAATCTCATTGATTTGCTT+AGG - chr4.3:36811689-36811708 None:intergenic 25.0%
!!! TTATTTCTGTTGGTTGTTAT+TGG + chr4.3:36811981-36812000 MS.gene056601:intron 25.0%
AATTCTCAAAAACTAACCTG+GGG - chr4.3:36811867-36811886 None:intergenic 30.0%
AGTGAAACATGAATGTAAGT+AGG - chr4.3:36812315-36812334 None:intergenic 30.0%
ATATTCTCCCCATATCTATT+TGG - chr4.3:36812081-36812100 None:intergenic 30.0%
GATCTGAATTAGGGAATTTA+TGG + chr4.3:36811500-36811519 MS.gene056601:intron 30.0%
TCATATTCATAAATCTGAGC+TGG + chr4.3:36812213-36812232 MS.gene056601:intron 30.0%
TGTAATGGCCAAATAGATAT+GGG + chr4.3:36812070-36812089 MS.gene056601:intron 30.0%
TTATGAATAAGAAATGGCAG+AGG - chr4.3:36812110-36812129 None:intergenic 30.0%
TTGGGAACAACTTATGATAT+GGG + chr4.3:36812165-36812184 MS.gene056601:intron 30.0%
ACAAAGTGTGTACAAGTACT+TGG - chr4.3:36811801-36811820 None:intergenic 35.0%
ATTCTCAAAAACTAACCTGG+GGG - chr4.3:36811866-36811885 None:intergenic 35.0%
CAAAGATGTTGTCAAGTTCA+AGG + chr4.3:36811766-36811785 MS.gene056601:CDS 35.0%
CTGTGTTGTGATTTGAAAGA+TGG + chr4.3:36811626-36811645 MS.gene056601:intron 35.0%
CTTCAACTTATCAGCTTTCT+CGG - chr4.3:36811834-36811853 None:intergenic 35.0%
GAAACACTGCAAAGAATCAA+TGG - chr4.3:36811344-36811363 None:intergenic 35.0%
GTAATGGCCAAATAGATATG+GGG + chr4.3:36812071-36812090 MS.gene056601:intron 35.0%
GTGTAATGGCCAAATAGATA+TGG + chr4.3:36812069-36812088 MS.gene056601:intron 35.0%
GTTGGGAACAACTTATGATA+TGG + chr4.3:36812164-36812183 MS.gene056601:intron 35.0%
TGGGAACAACTTATGATATG+GGG + chr4.3:36812166-36812185 MS.gene056601:intron 35.0%
TTTGCTTCTCTTGATCTTCA+CGG - chr4.3:36811750-36811769 None:intergenic 35.0%
! TTCTGTTGGTTGTTATTGGT+CGG + chr4.3:36811985-36812004 MS.gene056601:intron 35.0%
!! CATTCATGTTTCACTTTTGC+AGG + chr4.3:36812320-36812339 MS.gene056601:intron 35.0%
!!! GCTATTTTTCTCTCTCAGTT+GGG + chr4.3:36812147-36812166 MS.gene056601:intron 35.0%
!!! TGCTATTTTTCTCTCTCAGT+TGG + chr4.3:36812146-36812165 MS.gene056601:intron 35.0%
AGTACTTTCAACTCTAGGTG+AGG + chr4.3:36811935-36811954 MS.gene056601:intron 40.0%
GTGCAAGTACTTTCAACTCT+AGG + chr4.3:36811930-36811949 MS.gene056601:intron 40.0%
GTTATTGGTCGGAACCTTAA+AGG + chr4.3:36811996-36812015 MS.gene056601:intron 40.0%
TTGAAGCAATCACTTCCCCC+AGG + chr4.3:36811848-36811867 MS.gene056601:CDS 50.0%
ACTTCCTCCTCACTGCTAGG+AGG + chr4.3:36811711-36811730 MS.gene056601:CDS 55.0%
AGGACTTCCTCCTCACTGCT+AGG + chr4.3:36811708-36811727 MS.gene056601:CDS 55.0%
CATCCTTCCTCCTAGCAGTG+AGG - chr4.3:36811721-36811740 None:intergenic 55.0%
CCTCCTCACTGCTAGGAGGA+AGG + chr4.3:36811715-36811734 MS.gene056601:CDS 60.0%
CCTTCCTCCTAGCAGTGAGG+AGG - chr4.3:36811718-36811737 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 36811322 36812364 36811322 ID=MS.gene056601
chr4.3 mRNA 36811322 36812364 36811322 ID=MS.gene056601.t1;Parent=MS.gene056601
chr4.3 exon 36811322 36811324 36811322 ID=MS.gene056601.t1.exon1;Parent=MS.gene056601.t1
chr4.3 CDS 36811322 36811324 36811322 ID=cds.MS.gene056601.t1;Parent=MS.gene056601.t1
chr4.3 exon 36811686 36811869 36811686 ID=MS.gene056601.t1.exon2;Parent=MS.gene056601.t1
chr4.3 CDS 36811686 36811869 36811686 ID=cds.MS.gene056601.t1;Parent=MS.gene056601.t1
chr4.3 exon 36812342 36812364 36812342 ID=MS.gene056601.t1.exon3;Parent=MS.gene056601.t1
chr4.3 CDS 36812342 36812364 36812342 ID=cds.MS.gene056601.t1;Parent=MS.gene056601.t1
Gene Sequence

>MS.gene056601

ATGGTATTTTTCTCTATCTCCATTGATTCTTTGCAGTGTTTCTGTATATTTTTTTCAATTCACAGTTTTTTTTGTAGGAAATTGGAAGAATCTTAGCATTATCCTATCTTGATTTTCAATGTTTTTAGGGCTATCTGAACAAGTGAAATTGAATCAATGGAGTTTAGTATTATACATTGATCTGAATTAGGGAATTTATGGTTTTTGATTTTCAAACTTGGTAGTTGATAAAGTTGATAAAGGGAATGAAAATGCTTTGTTTTGATTATTATATTTTTTTTTATTTGGATTTGGATTTGGATTTCTGTGTTGTGATTTGAAAGATGGTTCACATTGTAACCTTTTTGTGTTTATTGAAATGTAGCCTAAGCAAATCAATGAGATTAAGGACTTCCTCCTCACTGCTAGGAGGAAGGATGCACGCTCCGTGAAGATCAAGAGAAGCAAAGATGTTGTCAAGTTCAAGGTTCGATGCTCCAAGTACTTGTACACACTTTGTGTGTTTGATACCGAGAAAGCTGATAAGTTGAAGCAATCACTTCCCCCAGGTTAGTTTTTGAGAATTATTGTTGGTTGTATGTTATGGTTGCTTTATAATTGTTATGAACGTGCAAGTACTTTCAACTCTAGGTGAGGCGCGAGTGAATTCCATAGATTGTTTATTTCTGTTGGTTGTTATTGGTCGGAACCTTAAAGGTGTAATAATTCTTTAAAAATGGAATCTATAAAGTAAATAATAAGAGGATTGTGTAATGGCCAAATAGATATGGGGAGAATATTGCATGCCTCTGCCATTTCTTATTCATAAATCTCAACTGTATTTCTGCTATTTTTCTCTCTCAGTTGGGAACAACTTATGATATGGGGAGAATATTGTATGCCTCTTCCATTTCATATTCATAAATCTGAGCTGGATTTGTTATTTTTCTCTCATTCTAGGAACAACTTATGAATTGATCATCAAGATGTAGAAATTTGAGTTTAGGTTATCCTACTTACATTCATGTTTCACTTTTGCAGGTTTGAATGTGCAAGACTTGTAA

Protein sequence

>MS.gene056601.t1

MPKQINEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDTEKADKLKQSLPPGLNVQDL