Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46292.t1 | XP_024627753.1 | 86.7 | 128 | 17 | 0 | 1 | 128 | 1 | 128 | 3.40E-53 | 217.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46292.t1 | B7FH71 | 66.9 | 133 | 41 | 2 | 1 | 131 | 1 | 132 | 2.8e-33 | 151.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049281 | MS.gene46292 | 0.824073 | 9.83E-54 | -1.69E-46 |
| MS.gene050363 | MS.gene46292 | 0.835285 | 1.83E-56 | -1.69E-46 |
| MS.gene050883 | MS.gene46292 | 0.804515 | 2.08E-49 | -1.69E-46 |
| MS.gene051946 | MS.gene46292 | 0.818958 | 1.49E-52 | -1.69E-46 |
| MS.gene053549 | MS.gene46292 | 0.832136 | 1.12E-55 | -1.69E-46 |
| MS.gene055549 | MS.gene46292 | 0.837735 | 4.36E-57 | -1.69E-46 |
| MS.gene055550 | MS.gene46292 | 0.846566 | 2.00E-59 | -1.69E-46 |
| MS.gene055551 | MS.gene46292 | 0.813925 | 2.00E-51 | -1.69E-46 |
| MS.gene056311 | MS.gene46292 | 0.820532 | 6.53E-53 | -1.69E-46 |
| MS.gene057014 | MS.gene46292 | 0.823842 | 1.11E-53 | -1.69E-46 |
| MS.gene058493 | MS.gene46292 | 0.832019 | 1.20E-55 | -1.69E-46 |
| MS.gene058494 | MS.gene46292 | 0.80905 | 2.29E-50 | -1.69E-46 |
| MS.gene058628 | MS.gene46292 | 0.807102 | 5.95E-50 | -1.69E-46 |
| MS.gene058630 | MS.gene46292 | 0.804249 | 2.36E-49 | -1.69E-46 |
| MS.gene060595 | MS.gene46292 | 0.803883 | 2.82E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 43 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCCCACCTGGGCCTGATGC+TGG | 0.216285 | 8.1:+36040278 | MS.gene46292:CDS |
| GGGGAAGATGTGTTTGGGCT+TGG | 0.239614 | 8.1:+36040323 | MS.gene46292:CDS |
| GGGAAGATGTGTTTGGGCTT+GGG | 0.240843 | 8.1:+36040324 | MS.gene46292:CDS |
| GTTTGTGATATTAGGTCTTT+TGG | 0.255388 | 8.1:-36040486 | None:intergenic |
| TGCTCTAGTCCGGGCCCACC+TGG | 0.276732 | 8.1:+36040266 | MS.gene46292:CDS |
| ATCAAATTGTTTGTGATATT+AGG | 0.276758 | 8.1:-36040494 | None:intergenic |
| GATGGAGCTGGTGCTGAATT+TGG | 0.295928 | 8.1:+36040410 | MS.gene46292:CDS |
| GCTGGTGGGGAAGATGTGTT+TGG | 0.322107 | 8.1:+36040317 | MS.gene46292:CDS |
| AGCTCCTGGCTGCTCTAGTC+CGG | 0.331077 | 8.1:+36040256 | MS.gene46292:CDS |
| TGCTGGAATCTTTGTGTCCA+TGG | 0.342466 | 8.1:-36040177 | None:intergenic |
| GAAGTTGAAGGAGGACTTGC+AGG | 0.343661 | 8.1:+36040359 | MS.gene46292:CDS |
| CCTTCAACTTCTTCTTCTCC+AGG | 0.349764 | 8.1:-36040347 | None:intergenic |
| GATGTGTTTGGGCTTGGGCC+TGG | 0.365493 | 8.1:+36040329 | MS.gene46292:CDS |
| GCCCACCTGGGCCTGATGCT+GGG | 0.385695 | 8.1:+36040279 | MS.gene46292:CDS |
| GCAGAGGGTGACTCAGCTCC+TGG | 0.403105 | 8.1:+36040242 | MS.gene46292:CDS |
| CTATTAGAGCCTGGTGCTGG+TGG | 0.429246 | 8.1:+36040302 | MS.gene46292:CDS |
| CTGGTGGGGAAGATGTGTTT+GGG | 0.437212 | 8.1:+36040318 | MS.gene46292:CDS |
| TATTAGAGCCTGGTGCTGGT+GGG | 0.447878 | 8.1:+36040303 | MS.gene46292:CDS |
| GGCTCTAATAGCCCAGCATC+AGG | 0.456286 | 8.1:-36040290 | None:intergenic |
| GCATCAGGCCCAGGTGGGCC+CGG | 0.462450 | 8.1:-36040275 | None:intergenic |
| GCTCCTGGCTGCTCTAGTCC+GGG | 0.489743 | 8.1:+36040257 | MS.gene46292:CDS |
| GGGCTATTAGAGCCTGGTGC+TGG | 0.490149 | 8.1:+36040299 | MS.gene46292:CDS |
| TTTGTGGAGGGTGATGGAGC+TGG | 0.491792 | 8.1:+36040398 | MS.gene46292:CDS |
| ATTAGAGCCTGGTGCTGGTG+GGG | 0.496717 | 8.1:+36040304 | MS.gene46292:CDS |
| GCTCTAGTCCGGGCCCACCT+GGG | 0.506944 | 8.1:+36040267 | MS.gene46292:CDS |
| GATGCTGGGCTATTAGAGCC+TGG | 0.508970 | 8.1:+36040293 | MS.gene46292:CDS |
| TGAAGGAGGACTTGCAGGAG+AGG | 0.526730 | 8.1:+36040364 | MS.gene46292:CDS |
| GATTGTGTGGGTGATGGTGC+AGG | 0.539483 | 8.1:+36040440 | MS.gene46292:CDS |
| CCTGGAGAAGAAGAAGTTGA+AGG | 0.541963 | 8.1:+36040347 | MS.gene46292:CDS |
| GATAATGATTGTGTGGGTGA+TGG | 0.545354 | 8.1:+36040434 | MS.gene46292:CDS |
| AATAGCCCAGCATCAGGCCC+AGG | 0.552354 | 8.1:-36040284 | None:intergenic |
| AGCCCAGCATCAGGCCCAGG+TGG | 0.566917 | 8.1:-36040281 | None:intergenic |
| GGACTTGCAGGAGAGGGTGT+TGG | 0.579194 | 8.1:+36040371 | MS.gene46292:CDS |
| GGGCCCGGACTAGAGCAGCC+AGG | 0.581693 | 8.1:-36040260 | None:intergenic |
| GAAGGAGGACTTGCAGGAGA+GGG | 0.595128 | 8.1:+36040365 | MS.gene46292:CDS |
| ATTTGGTGATAATGATTGTG+TGG | 0.597910 | 8.1:+36040427 | MS.gene46292:CDS |
| AGTAACAGCAACAAAAGTGT+TGG | 0.609923 | 8.1:+36040205 | MS.gene46292:CDS |
| ACATCTTCCCCACCAGCACC+AGG | 0.610370 | 8.1:-36040311 | None:intergenic |
| GTGATAGAGAATGCAGCAGA+GGG | 0.615284 | 8.1:+36040227 | MS.gene46292:CDS |
| GCCCAGCATCAGGCCCAGGT+GGG | 0.625332 | 8.1:-36040280 | None:intergenic |
| TTTGGTGATAATGATTGTGT+GGG | 0.641938 | 8.1:+36040428 | MS.gene46292:CDS |
| GGAGAAGAAGAAGTTGAAGG+AGG | 0.655208 | 8.1:+36040350 | MS.gene46292:CDS |
| GGTGATAGAGAATGCAGCAG+AGG | 0.711837 | 8.1:+36040226 | MS.gene46292:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCAAATTGTTTGTGATATT+AGG | - | chr8.1:36040497-36040516 | None:intergenic | 20.0% |
| ! | TCGAAAAACATATACAACAT+GGG | - | chr8.1:36040530-36040549 | None:intergenic | 25.0% |
| CTCGAAAAACATATACAACA+TGG | - | chr8.1:36040531-36040550 | None:intergenic | 30.0% | |
| ! | ATTTGGTGATAATGATTGTG+TGG | + | chr8.1:36040427-36040446 | MS.gene46292:CDS | 30.0% |
| ! | CATGTTGTATATGTTTTTCG+AGG | + | chr8.1:36040529-36040548 | MS.gene46292:CDS | 30.0% |
| ! | GTTTGTGATATTAGGTCTTT+TGG | - | chr8.1:36040489-36040508 | None:intergenic | 30.0% |
| ! | TTTGGTGATAATGATTGTGT+GGG | + | chr8.1:36040428-36040447 | MS.gene46292:CDS | 30.0% |
| ! | AGTAACAGCAACAAAAGTGT+TGG | + | chr8.1:36040205-36040224 | MS.gene46292:CDS | 35.0% |
| GATAATGATTGTGTGGGTGA+TGG | + | chr8.1:36040434-36040453 | MS.gene46292:CDS | 40.0% | |
| CCTGGAGAAGAAGAAGTTGA+AGG | + | chr8.1:36040347-36040366 | MS.gene46292:CDS | 45.0% | |
| CCTTCAACTTCTTCTTCTCC+AGG | - | chr8.1:36040350-36040369 | None:intergenic | 45.0% | |
| GGAGAAGAAGAAGTTGAAGG+AGG | + | chr8.1:36040350-36040369 | MS.gene46292:CDS | 45.0% | |
| GTGATAGAGAATGCAGCAGA+GGG | + | chr8.1:36040227-36040246 | MS.gene46292:CDS | 45.0% | |
| !! | GTTGCTGTTACTGCTTTTGC+TGG | - | chr8.1:36040197-36040216 | None:intergenic | 45.0% |
| !!! | AGAGGGTGTTGGTGATTTTG+TGG | + | chr8.1:36040382-36040401 | MS.gene46292:CDS | 45.0% |
| !!! | GGTGTTGGTGATTTTGTGGA+GGG | + | chr8.1:36040386-36040405 | MS.gene46292:CDS | 45.0% |
| CTGGTGGGGAAGATGTGTTT+GGG | + | chr8.1:36040318-36040337 | MS.gene46292:CDS | 50.0% | |
| GAAGTTGAAGGAGGACTTGC+AGG | + | chr8.1:36040359-36040378 | MS.gene46292:CDS | 50.0% | |
| GGGAAGATGTGTTTGGGCTT+GGG | + | chr8.1:36040324-36040343 | MS.gene46292:CDS | 50.0% | |
| GGTGATAGAGAATGCAGCAG+AGG | + | chr8.1:36040226-36040245 | MS.gene46292:CDS | 50.0% | |
| ! | GATGGAGCTGGTGCTGAATT+TGG | + | chr8.1:36040410-36040429 | MS.gene46292:CDS | 50.0% |
| ! | GGTGATTTTGTGGAGGGTGA+TGG | + | chr8.1:36040392-36040411 | MS.gene46292:CDS | 50.0% |
| !! | TATTAGAGCCTGGTGCTGGT+GGG | + | chr8.1:36040303-36040322 | MS.gene46292:CDS | 50.0% |
| !!! | GGGTGTTGGTGATTTTGTGG+AGG | + | chr8.1:36040385-36040404 | MS.gene46292:CDS | 50.0% |
| GAAGGAGGACTTGCAGGAGA+GGG | + | chr8.1:36040365-36040384 | MS.gene46292:CDS | 55.0% | |
| GATGCTGGGCTATTAGAGCC+TGG | + | chr8.1:36040293-36040312 | MS.gene46292:CDS | 55.0% | |
| GCTGGTGGGGAAGATGTGTT+TGG | + | chr8.1:36040317-36040336 | MS.gene46292:CDS | 55.0% | |
| GGGGAAGATGTGTTTGGGCT+TGG | + | chr8.1:36040323-36040342 | MS.gene46292:CDS | 55.0% | |
| TGAAGGAGGACTTGCAGGAG+AGG | + | chr8.1:36040364-36040383 | MS.gene46292:CDS | 55.0% | |
| ! | CTATTAGAGCCTGGTGCTGG+TGG | + | chr8.1:36040302-36040321 | MS.gene46292:CDS | 55.0% |
| ! | GATTGTGTGGGTGATGGTGC+AGG | + | chr8.1:36040440-36040459 | MS.gene46292:CDS | 55.0% |
| ! | GGCTCTAATAGCCCAGCATC+AGG | - | chr8.1:36040293-36040312 | None:intergenic | 55.0% |
| !! | ATTAGAGCCTGGTGCTGGTG+GGG | + | chr8.1:36040304-36040323 | MS.gene46292:CDS | 55.0% |
| !! | TTTGTGGAGGGTGATGGAGC+TGG | + | chr8.1:36040398-36040417 | MS.gene46292:CDS | 55.0% |
| AATAGCCCAGCATCAGGCCC+AGG | - | chr8.1:36040287-36040306 | None:intergenic | 60.0% | |
| ACATCTTCCCCACCAGCACC+AGG | - | chr8.1:36040314-36040333 | None:intergenic | 60.0% | |
| AGCTCCTGGCTGCTCTAGTC+CGG | + | chr8.1:36040256-36040275 | MS.gene46292:CDS | 60.0% | |
| GATGTGTTTGGGCTTGGGCC+TGG | + | chr8.1:36040329-36040348 | MS.gene46292:CDS | 60.0% | |
| GGGCTATTAGAGCCTGGTGC+TGG | + | chr8.1:36040299-36040318 | MS.gene46292:CDS | 60.0% | |
| ! | GGACTTGCAGGAGAGGGTGT+TGG | + | chr8.1:36040371-36040390 | MS.gene46292:CDS | 60.0% |
| GCAGAGGGTGACTCAGCTCC+TGG | + | chr8.1:36040242-36040261 | MS.gene46292:CDS | 65.0% | |
| GCTCCTGGCTGCTCTAGTCC+GGG | + | chr8.1:36040257-36040276 | MS.gene46292:CDS | 65.0% | |
| AGCCCAGCATCAGGCCCAGG+TGG | - | chr8.1:36040284-36040303 | None:intergenic | 70.0% | |
| GCCCACCTGGGCCTGATGCT+GGG | + | chr8.1:36040279-36040298 | MS.gene46292:CDS | 70.0% | |
| GCCCAGCATCAGGCCCAGGT+GGG | - | chr8.1:36040283-36040302 | None:intergenic | 70.0% | |
| GCTCTAGTCCGGGCCCACCT+GGG | + | chr8.1:36040267-36040286 | MS.gene46292:CDS | 70.0% | |
| TGCTCTAGTCCGGGCCCACC+TGG | + | chr8.1:36040266-36040285 | MS.gene46292:CDS | 70.0% | |
| GCATCAGGCCCAGGTGGGCC+CGG | - | chr8.1:36040278-36040297 | None:intergenic | 75.0% | |
| GGCCCACCTGGGCCTGATGC+TGG | + | chr8.1:36040278-36040297 | MS.gene46292:CDS | 75.0% | |
| ! | GGGCCCGGACTAGAGCAGCC+AGG | - | chr8.1:36040263-36040282 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 36040179 | 36040580 | 36040179 | ID=MS.gene46292 |
| chr8.1 | mRNA | 36040179 | 36040580 | 36040179 | ID=MS.gene46292.t1;Parent=MS.gene46292 |
| chr8.1 | exon | 36040179 | 36040580 | 36040179 | ID=MS.gene46292.t1.exon1;Parent=MS.gene46292.t1 |
| chr8.1 | CDS | 36040179 | 36040580 | 36040179 | ID=cds.MS.gene46292.t1;Parent=MS.gene46292.t1 |
| Gene Sequence |
| Protein sequence |
