Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48650.t1 | XP_003605540.1 | 89.2 | 474 | 45 | 1 | 1 | 474 | 1 | 468 | 4.00E-237 | 830.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48650.t1 | F4HYD7 | 67.2 | 418 | 135 | 1 | 57 | 472 | 50 | 467 | 3.0e-169 | 596.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48650.t1 | G7JP72 | 89.2 | 474 | 45 | 1 | 1 | 474 | 1 | 468 | 2.9e-237 | 830.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene48650 | 0.80238 | 5.77E-49 | -1.69E-46 |
| MS.gene051332 | MS.gene48650 | 0.809545 | 1.79E-50 | -1.69E-46 |
| MS.gene052674 | MS.gene48650 | 0.80886 | 2.52E-50 | -1.69E-46 |
| MS.gene052675 | MS.gene48650 | 0.808089 | 3.68E-50 | -1.69E-46 |
| MS.gene052677 | MS.gene48650 | 0.806588 | 7.65E-50 | -1.69E-46 |
| MS.gene053272 | MS.gene48650 | -0.823954 | 1.05E-53 | -1.69E-46 |
| MS.gene054479 | MS.gene48650 | 0.808986 | 2.37E-50 | -1.69E-46 |
| MS.gene056539 | MS.gene48650 | 0.804001 | 2.66E-49 | -1.69E-46 |
| MS.gene057208 | MS.gene48650 | 0.806753 | 7.06E-50 | -1.69E-46 |
| MS.gene058159 | MS.gene48650 | 0.816413 | 5.60E-52 | -1.69E-46 |
| MS.gene058223 | MS.gene48650 | -0.811927 | 5.48E-51 | -1.69E-46 |
| MS.gene060527 | MS.gene48650 | 0.80772 | 4.40E-50 | -1.69E-46 |
| MS.gene060777 | MS.gene48650 | 0.937492 | 3.17E-98 | -1.69E-46 |
| MS.gene060932 | MS.gene48650 | 0.807337 | 5.31E-50 | -1.69E-46 |
| MS.gene060935 | MS.gene48650 | 0.812306 | 4.53E-51 | -1.69E-46 |
| MS.gene060937 | MS.gene48650 | 0.823448 | 1.38E-53 | -1.69E-46 |
| MS.gene061160 | MS.gene48650 | 0.832891 | 7.30E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48650.t1 | MTR_4g032800 | 96.624 | 474 | 10 | 1 | 1 | 474 | 1 | 468 | 0.0 | 946 |
| MS.gene48650.t1 | MTR_1g019870 | 28.571 | 217 | 129 | 6 | 246 | 460 | 9 | 201 | 8.62e-13 | 67.8 |
| MS.gene48650.t1 | MTR_2g012810 | 29.885 | 174 | 107 | 4 | 258 | 430 | 4 | 163 | 1.41e-11 | 63.9 |
| MS.gene48650.t1 | MTR_2g012810 | 29.885 | 174 | 107 | 4 | 258 | 430 | 46 | 205 | 4.18e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48650.t1 | AT1G35470 | 64.270 | 459 | 157 | 2 | 16 | 472 | 14 | 467 | 0.0 | 628 |
| MS.gene48650.t1 | AT1G35470 | 63.834 | 459 | 157 | 3 | 16 | 472 | 14 | 465 | 0.0 | 619 |
| MS.gene48650.t1 | AT4G09340 | 67.062 | 422 | 134 | 2 | 49 | 470 | 21 | 437 | 0.0 | 582 |
| MS.gene48650.t1 | AT4G09200 | 42.184 | 403 | 208 | 10 | 57 | 452 | 12 | 396 | 4.39e-97 | 298 |
| MS.gene48650.t1 | AT4G09310 | 42.184 | 403 | 208 | 10 | 57 | 452 | 12 | 396 | 4.39e-97 | 298 |
| MS.gene48650.t1 | AT4G09250 | 37.717 | 403 | 178 | 10 | 57 | 452 | 12 | 348 | 2.07e-78 | 248 |
| MS.gene48650.t1 | AT1G06060 | 29.302 | 215 | 130 | 5 | 246 | 460 | 7 | 199 | 1.94e-16 | 78.6 |
| MS.gene48650.t1 | AT1G06060 | 29.167 | 216 | 131 | 5 | 246 | 461 | 7 | 200 | 2.19e-16 | 78.2 |
Find 103 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTAAGGATGCTGGTGTTAA+AGG | 0.230981 | 4.3:-67624266 | MS.gene48650:CDS |
| TTAGGACTGTGTTGCTTTGC+TGG | 0.236098 | 4.3:-67613495 | MS.gene48650:intron |
| TTGTTTCAATGAAGCTAATC+AGG | 0.293085 | 4.3:-67616415 | MS.gene48650:intron |
| CTGGAGGAGGCTGTCAAATA+TGG | 0.316922 | 4.3:-67613879 | MS.gene48650:CDS |
| CAGGGTAAAGGGGAAGCTTT+TGG | 0.318442 | 4.3:-67623034 | MS.gene48650:CDS |
| TACGACTCAATTCTAAGAAA+TGG | 0.325466 | 4.3:+67624516 | None:intergenic |
| CTTTACCCTGTCCACGATAA+AGG | 0.331990 | 4.3:+67623047 | None:intergenic |
| AAAATTTATCGAACTAGTTC+GGG | 0.335513 | 4.3:-67615148 | MS.gene48650:intron |
| TCTCTTTGAGGATTTAGTTC+AGG | 0.348395 | 4.3:-67613823 | MS.gene48650:intron |
| GATAGCACACCTCAGCAATC+TGG | 0.361401 | 4.3:+67616345 | None:intergenic |
| TCAACGCATATGTTGTTTCC+TGG | 0.370298 | 4.3:+67617198 | None:intergenic |
| TTTATCGTGGACAGGGTAAA+GGG | 0.407139 | 4.3:-67623045 | MS.gene48650:CDS |
| TATACGAGTGTGAATCTTCA+TGG | 0.407792 | 4.3:-67624362 | MS.gene48650:CDS |
| ACCATTCACCTTTGACCTAA+AGG | 0.407822 | 4.3:-67621150 | MS.gene48650:intron |
| AACAGGGTACATGTCAACTT+TGG | 0.412192 | 4.3:-67621179 | MS.gene48650:intron |
| TCTCACCCATAACTGGCATT+TGG | 0.412599 | 4.3:+67620132 | None:intergenic |
| AACAGCAACAGTTGGGAAAA+GGG | 0.414213 | 4.3:+67622411 | None:intergenic |
| TGTTGCTACTGATAAGCTTT+CGG | 0.414522 | 4.3:-67624390 | MS.gene48650:CDS |
| CACAGTTTGTAAAGAAATGA+AGG | 0.414745 | 4.3:-67622437 | MS.gene48650:CDS |
| CAAATTCAAAATCAAGATCT+AGG | 0.414891 | 4.3:-67624542 | MS.gene48650:CDS |
| CTTTATCGTGGACAGGGTAA+AGG | 0.419684 | 4.3:-67623046 | MS.gene48650:CDS |
| GAACAGCAACAGTTGGGAAA+AGG | 0.429415 | 4.3:+67622410 | None:intergenic |
| AACTATATCACCAGTGGTAT+AGG | 0.432201 | 4.3:+67623006 | None:intergenic |
| GTACAACATACTCTTCATTC+TGG | 0.435067 | 4.3:+67622383 | None:intergenic |
| GTTAAAGGGCAGGTTTCGAT+TGG | 0.438082 | 4.3:-67624251 | MS.gene48650:CDS |
| AGATTCCAATGGTCGTTCAT+AGG | 0.439236 | 4.3:+67613472 | None:intergenic |
| TCTTAGTAACCTCTCAAGAT+TGG | 0.443952 | 4.3:+67613337 | None:intergenic |
| TTAAGGATGCTGGTGTTAAA+GGG | 0.450841 | 4.3:-67624265 | MS.gene48650:CDS |
| GGGGAGATTGATGCTGCATT+TGG | 0.451426 | 4.3:-67616389 | MS.gene48650:CDS |
| ACCTTTAGGTCAAAGGTGAA+TGG | 0.451440 | 4.3:+67621149 | None:intergenic |
| TGGTTTCCTTTATCGTGGAC+AGG | 0.453833 | 4.3:-67623053 | MS.gene48650:CDS |
| TGGAATTAACTATGCTGCTC+AGG | 0.455934 | 4.3:-67622978 | MS.gene48650:CDS |
| ACAGGGTACATGTCAACTTT+GGG | 0.463817 | 4.3:-67621178 | MS.gene48650:intron |
| ATAGCACACCTCAGCAATCT+GGG | 0.466738 | 4.3:+67616346 | None:intergenic |
| AGTTGATGATGAAGAGAAAG+AGG | 0.469005 | 4.3:-67624462 | MS.gene48650:CDS |
| ACCTCTCAAGATTGGAGTGC+AGG | 0.472604 | 4.3:+67613345 | None:intergenic |
| GTGTCACCAAATGCCAGTTA+TGG | 0.473579 | 4.3:-67620138 | MS.gene48650:CDS |
| CGTTCCTACCTGCTACACTA+TGG | 0.480483 | 4.3:-67617308 | MS.gene48650:CDS |
| CTTCATAGCCATAGTGTAGC+AGG | 0.480538 | 4.3:+67617300 | None:intergenic |
| CATTTGGTAAATTGCGTGAA+TGG | 0.481704 | 4.3:-67616373 | MS.gene48650:CDS |
| TGTCACCAAATGCCAGTTAT+GGG | 0.494644 | 4.3:-67620137 | MS.gene48650:intron |
| TGGCTATGAACAGCAACAGT+TGG | 0.497068 | 4.3:+67622403 | None:intergenic |
| GGCTATGAACAGCAACAGTT+GGG | 0.498761 | 4.3:+67622404 | None:intergenic |
| TGATATTTAGGTGGGATCAC+TGG | 0.502295 | 4.3:-67613898 | MS.gene48650:intron |
| TTGAATACGATAAATTCATC+AGG | 0.504742 | 4.3:-67624425 | MS.gene48650:CDS |
| TGTGGATATCACGGGGATGA+TGG | 0.505619 | 4.3:-67623073 | MS.gene48650:CDS |
| CACTATATTTATAGGAGGAA+TGG | 0.511735 | 4.3:+67617241 | None:intergenic |
| CTAATAGCTGTGGATATCAC+GGG | 0.512447 | 4.3:-67623081 | MS.gene48650:CDS |
| TTAAAGGGCAGGTTTCGATT+GGG | 0.512635 | 4.3:-67624250 | MS.gene48650:CDS |
| GCTGGCCTATGAACGACCAT+TGG | 0.514869 | 4.3:-67613477 | MS.gene48650:CDS |
| ATTCATTTGACGAGGCTAGC+AGG | 0.517525 | 4.3:-67617268 | MS.gene48650:CDS |
| GGATGCTGGTGTTAAAGGGC+AGG | 0.521810 | 4.3:-67624261 | MS.gene48650:CDS |
| CGATCGTTGAGTGGAGAGCA+AGG | 0.522062 | 4.3:-67613287 | MS.gene48650:CDS |
| CAATGAAGCTAATCAGGGAT+GGG | 0.526869 | 4.3:-67616409 | MS.gene48650:intron |
| AGTTCACACCTTTAGGTCAA+AGG | 0.532522 | 4.3:+67621142 | None:intergenic |
| ATAAATATAGTGCAAGAAAA+CGG | 0.535203 | 4.3:-67617230 | MS.gene48650:CDS |
| GCTAATAGCTGTGGATATCA+CGG | 0.539147 | 4.3:-67623082 | MS.gene48650:CDS |
| TATCATTGCATTTACTGCAT+CGG | 0.540152 | 4.3:+67613406 | None:intergenic |
| GATGATGGTTTCCTTTATCG+TGG | 0.540400 | 4.3:-67623058 | MS.gene48650:CDS |
| TATACCACTGGTGATATAGT+TGG | 0.543774 | 4.3:-67623004 | MS.gene48650:CDS |
| CGACCATTGGAATCTGCCGT+TGG | 0.547674 | 4.3:-67613464 | MS.gene48650:CDS |
| AATCTTCATGGTCATGATGT+TGG | 0.548801 | 4.3:-67624350 | MS.gene48650:CDS |
| TAATAAACAATCCTCTTCAT+CGG | 0.552789 | 4.3:+67624302 | None:intergenic |
| TGTTTCAATGAAGCTAATCA+GGG | 0.556130 | 4.3:-67616414 | MS.gene48650:intron |
| TTTGGTCCAACCTATACCAC+TGG | 0.557020 | 4.3:-67623016 | MS.gene48650:CDS |
| ATAAAGTTGCACCGATGAAG+AGG | 0.558081 | 4.3:-67624313 | MS.gene48650:CDS |
| TCTTGCACTATATTTATAGG+AGG | 0.562030 | 4.3:+67617236 | None:intergenic |
| TCAATGAAGCTAATCAGGGA+TGG | 0.567235 | 4.3:-67616410 | MS.gene48650:intron |
| ACTGGTGATATAGTTGGTGC+TGG | 0.567921 | 4.3:-67622998 | MS.gene48650:CDS |
| AATACGATAAATTCATCAGG+TGG | 0.579812 | 4.3:-67624422 | MS.gene48650:CDS |
| TTTAAGATGAGGAGACAACC+TGG | 0.587971 | 4.3:-67624212 | MS.gene48650:CDS |
| AGAAAACGGTATTGATGACC+AGG | 0.594424 | 4.3:-67617216 | MS.gene48650:CDS |
| GGTTTCCTTTATCGTGGACA+GGG | 0.594845 | 4.3:-67623052 | MS.gene48650:CDS |
| ATATCACCAGTGGTATAGGT+TGG | 0.595825 | 4.3:+67623010 | None:intergenic |
| GCCTGCACTCCAATCTTGAG+AGG | 0.598798 | 4.3:-67613346 | MS.gene48650:CDS |
| TTAGGTGGGATCACTGGAGG+AGG | 0.600622 | 4.3:-67613892 | MS.gene48650:intron |
| TCACAGAAAGACTCTACGGC+AGG | 0.601062 | 4.3:-67617174 | MS.gene48650:intron |
| AATGAAGCTAATCAGGGATG+GGG | 0.603128 | 4.3:-67616408 | MS.gene48650:intron |
| ACAGCAACAGTTGGGAAAAG+GGG | 0.605059 | 4.3:+67622412 | None:intergenic |
| TTAGAGAGACGATCGTTGAG+TGG | 0.615906 | 4.3:-67613296 | MS.gene48650:CDS |
| TGAATCACAGAAAGACTCTA+CGG | 0.618580 | 4.3:-67617178 | MS.gene48650:CDS |
| TACATTGAATTCATTTGACG+AGG | 0.619444 | 4.3:-67617276 | MS.gene48650:CDS |
| TAACCAACGGCAGATTCCAA+TGG | 0.620079 | 4.3:+67613461 | None:intergenic |
| AGGTGGGAGGCTAATAGCTG+TGG | 0.620718 | 4.3:-67623091 | MS.gene48650:CDS |
| GTCTTTCAGAAGATAACCAA+CGG | 0.636109 | 4.3:+67613448 | None:intergenic |
| CATAGTGTAGCAGGTAGGAA+CGG | 0.636160 | 4.3:+67617309 | None:intergenic |
| TAACTGGCATTTGGTGACAC+AGG | 0.640519 | 4.3:+67620141 | None:intergenic |
| CTTCAAAGAGTGCTTAGCAG+TGG | 0.641720 | 4.3:-67613251 | MS.gene48650:CDS |
| GTTATTTGATGATAGGTGGG+AGG | 0.644271 | 4.3:-67623104 | MS.gene48650:intron |
| ACAGTTTGTAAAGAAATGAA+GGG | 0.648698 | 4.3:-67622436 | MS.gene48650:CDS |
| TTTATCGTATTCAACTCACT+CGG | 0.656213 | 4.3:+67624434 | None:intergenic |
| CAATCAATGAAAGAAACCAA+CGG | 0.657872 | 4.3:-67624608 | None:intergenic |
| TGAACTAAATCCTCAAAGAG+AGG | 0.658307 | 4.3:+67613825 | None:intergenic |
| GAAGCAACAAATCAAAACAG+AGG | 0.661214 | 4.3:-67620169 | MS.gene48650:CDS |
| TTATCGTGGACAGGGTAAAG+GGG | 0.668081 | 4.3:-67623044 | MS.gene48650:CDS |
| TATTTAGGTGGGATCACTGG+AGG | 0.670003 | 4.3:-67613895 | MS.gene48650:intron |
| TTAAGATGAGGAGACAACCT+GGG | 0.670911 | 4.3:-67624211 | MS.gene48650:intron |
| TAGCACACCTCAGCAATCTG+GGG | 0.678597 | 4.3:+67616347 | None:intergenic |
| AGCACCAACTATATCACCAG+TGG | 0.702372 | 4.3:+67623000 | None:intergenic |
| GGTTTCGATTGGGTTTACGA+CGG | 0.708370 | 4.3:-67624240 | MS.gene48650:CDS |
| ATAGCCATAGTGTAGCAGGT+AGG | 0.718923 | 4.3:+67617304 | None:intergenic |
| ATGGTATCCCCAGATTGCTG+AGG | 0.724330 | 4.3:-67616354 | MS.gene48650:intron |
| TAATAGCTGTGGATATCACG+GGG | 0.725260 | 4.3:-67623080 | MS.gene48650:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 67613238 | 67624624 | 67613238 | ID=MS.gene48650 |
| chr4.3 | mRNA | 67613238 | 67624624 | 67613238 | ID=MS.gene48650.t1;Parent=MS.gene48650 |
| chr4.3 | exon | 67624212 | 67624624 | 67624212 | ID=MS.gene48650.t1.exon1;Parent=MS.gene48650.t1 |
| chr4.3 | CDS | 67624212 | 67624624 | 67624212 | ID=cds.MS.gene48650.t1;Parent=MS.gene48650.t1 |
| chr4.3 | exon | 67622965 | 67623111 | 67622965 | ID=MS.gene48650.t1.exon2;Parent=MS.gene48650.t1 |
| chr4.3 | CDS | 67622965 | 67623111 | 67622965 | ID=cds.MS.gene48650.t1;Parent=MS.gene48650.t1 |
| chr4.3 | exon | 67622394 | 67622480 | 67622394 | ID=MS.gene48650.t1.exon3;Parent=MS.gene48650.t1 |
| chr4.3 | CDS | 67622394 | 67622480 | 67622394 | ID=cds.MS.gene48650.t1;Parent=MS.gene48650.t1 |
| chr4.3 | exon | 67621151 | 67621196 | 67621151 | ID=MS.gene48650.t1.exon4;Parent=MS.gene48650.t1 |
| chr4.3 | CDS | 67621151 | 67621196 | 67621151 | ID=cds.MS.gene48650.t1;Parent=MS.gene48650.t1 |
| chr4.3 | exon | 67620138 | 67620214 | 67620138 | ID=MS.gene48650.t1.exon5;Parent=MS.gene48650.t1 |
| chr4.3 | CDS | 67620138 | 67620214 | 67620138 | ID=cds.MS.gene48650.t1;Parent=MS.gene48650.t1 |
| chr4.3 | exon | 67617175 | 67617337 | 67617175 | ID=MS.gene48650.t1.exon6;Parent=MS.gene48650.t1 |
| chr4.3 | CDS | 67617175 | 67617337 | 67617175 | ID=cds.MS.gene48650.t1;Parent=MS.gene48650.t1 |
| chr4.3 | exon | 67616355 | 67616423 | 67616355 | ID=MS.gene48650.t1.exon7;Parent=MS.gene48650.t1 |
| chr4.3 | CDS | 67616355 | 67616423 | 67616355 | ID=cds.MS.gene48650.t1;Parent=MS.gene48650.t1 |
| chr4.3 | exon | 67615149 | 67615208 | 67615149 | ID=MS.gene48650.t1.exon8;Parent=MS.gene48650.t1 |
| chr4.3 | CDS | 67615149 | 67615208 | 67615149 | ID=cds.MS.gene48650.t1;Parent=MS.gene48650.t1 |
| chr4.3 | exon | 67613824 | 67613910 | 67613824 | ID=MS.gene48650.t1.exon9;Parent=MS.gene48650.t1 |
| chr4.3 | CDS | 67613824 | 67613910 | 67613824 | ID=cds.MS.gene48650.t1;Parent=MS.gene48650.t1 |
| chr4.3 | exon | 67613238 | 67613513 | 67613238 | ID=MS.gene48650.t1.exon10;Parent=MS.gene48650.t1 |
| chr4.3 | CDS | 67613238 | 67613513 | 67613238 | ID=cds.MS.gene48650.t1;Parent=MS.gene48650.t1 |
| Gene Sequence |
| Protein sequence |
