Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49158.t1 | XP_003592191.1 | 93.4 | 136 | 8 | 1 | 1 | 135 | 1 | 136 | 5.20E-49 | 203.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49158.t1 | O03982 | 57.5 | 73 | 30 | 1 | 1 | 72 | 1 | 73 | 1.9e-15 | 83.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49158.t1 | G7IDY9 | 93.4 | 136 | 8 | 1 | 1 | 135 | 1 | 136 | 3.7e-49 | 203.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050961 | MS.gene49158 | 0.805479 | 1.31E-49 | -1.69E-46 |
| MS.gene051959 | MS.gene49158 | 0.81371 | 2.23E-51 | -1.69E-46 |
| MS.gene051960 | MS.gene49158 | 0.801142 | 1.03E-48 | -1.69E-46 |
| MS.gene054892 | MS.gene49158 | 0.819574 | 1.08E-52 | -1.69E-46 |
| MS.gene054893 | MS.gene49158 | 0.815567 | 8.66E-52 | -1.69E-46 |
| MS.gene06023 | MS.gene49158 | 0.808279 | 3.35E-50 | -1.69E-46 |
| MS.gene06061 | MS.gene49158 | 0.805625 | 1.22E-49 | -1.69E-46 |
| MS.gene061047 | MS.gene49158 | 0.808651 | 2.79E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49158.t1 | MTR_1g099800 | 95.588 | 136 | 5 | 1 | 1 | 135 | 1 | 136 | 6.74e-92 | 262 |
| MS.gene49158.t1 | MTR_1g099810 | 79.286 | 140 | 23 | 4 | 1 | 135 | 1 | 139 | 3.61e-58 | 177 |
| MS.gene49158.t1 | MTR_1g102240 | 81.481 | 135 | 22 | 2 | 1 | 135 | 1 | 132 | 2.94e-56 | 172 |
| MS.gene49158.t1 | MTR_1g099815 | 81.481 | 135 | 22 | 2 | 1 | 135 | 1 | 132 | 1.22e-55 | 170 |
| MS.gene49158.t1 | MTR_1g099810 | 76.860 | 121 | 22 | 4 | 20 | 135 | 1 | 120 | 2.32e-45 | 144 |
| MS.gene49158.t1 | MTR_6g049280 | 55.970 | 134 | 48 | 2 | 1 | 134 | 31 | 153 | 1.19e-36 | 123 |
| MS.gene49158.t1 | MTR_6g049280 | 56.716 | 134 | 47 | 2 | 1 | 134 | 1 | 123 | 1.35e-36 | 122 |
| MS.gene49158.t1 | MTR_6g051690 | 57.037 | 135 | 48 | 3 | 1 | 134 | 1 | 126 | 3.45e-36 | 120 |
| MS.gene49158.t1 | MTR_6g049320 | 55.970 | 134 | 48 | 2 | 1 | 134 | 31 | 153 | 1.32e-35 | 120 |
| MS.gene49158.t1 | MTR_6g049320 | 56.716 | 134 | 47 | 2 | 1 | 134 | 1 | 123 | 1.73e-35 | 119 |
| MS.gene49158.t1 | MTR_1g099810 | 73.333 | 105 | 22 | 4 | 36 | 135 | 1 | 104 | 7.59e-34 | 114 |
| MS.gene49158.t1 | MTR_8g098915 | 46.715 | 137 | 59 | 3 | 1 | 134 | 1 | 126 | 1.04e-32 | 112 |
| MS.gene49158.t1 | MTR_6g051680 | 74.286 | 70 | 18 | 0 | 1 | 70 | 1 | 70 | 2.35e-32 | 110 |
| MS.gene49158.t1 | MTR_1g099640 | 54.478 | 134 | 56 | 2 | 1 | 134 | 1 | 129 | 2.81e-31 | 108 |
| MS.gene49158.t1 | MTR_7g451210 | 49.565 | 115 | 43 | 2 | 1 | 115 | 1 | 100 | 9.03e-30 | 104 |
| MS.gene49158.t1 | MTR_3g434890 | 48.696 | 115 | 44 | 2 | 1 | 115 | 1 | 100 | 1.90e-29 | 103 |
| MS.gene49158.t1 | MTR_3g434960 | 48.696 | 115 | 44 | 2 | 1 | 115 | 1 | 100 | 3.17e-29 | 102 |
| MS.gene49158.t1 | MTR_7g451200 | 43.902 | 123 | 56 | 1 | 1 | 123 | 1 | 110 | 1.28e-28 | 101 |
| MS.gene49158.t1 | MTR_6g049260 | 61.842 | 76 | 29 | 0 | 1 | 76 | 1 | 76 | 2.00e-28 | 100 |
| MS.gene49158.t1 | MTR_0795s0020 | 51.079 | 139 | 50 | 4 | 1 | 134 | 1 | 126 | 2.18e-28 | 101 |
| MS.gene49158.t1 | MTR_1173s0010 | 63.158 | 76 | 28 | 0 | 1 | 76 | 1 | 76 | 2.74e-28 | 100 |
| MS.gene49158.t1 | MTR_3g435000 | 46.364 | 110 | 44 | 1 | 6 | 115 | 10 | 104 | 5.04e-26 | 94.7 |
| MS.gene49158.t1 | MTR_8g098915 | 44.068 | 118 | 52 | 3 | 20 | 134 | 1 | 107 | 6.04e-24 | 89.4 |
| MS.gene49158.t1 | MTR_7g079110 | 57.353 | 68 | 29 | 0 | 1 | 68 | 1 | 68 | 3.11e-22 | 86.7 |
| MS.gene49158.t1 | MTR_3g434920 | 46.591 | 88 | 32 | 2 | 28 | 115 | 2 | 74 | 2.84e-18 | 74.3 |
| MS.gene49158.t1 | MTR_1g099640 | 50.505 | 99 | 44 | 2 | 36 | 134 | 1 | 94 | 1.38e-13 | 62.4 |
| MS.gene49158.t1 | MTR_3g082250 | 44.444 | 81 | 40 | 4 | 2 | 77 | 4 | 84 | 2.45e-13 | 62.4 |
| MS.gene49158.t1 | MTR_8g028615 | 44.595 | 148 | 68 | 3 | 1 | 134 | 1 | 148 | 1.18e-12 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49158.t1 | AT5G52740 | 44.776 | 134 | 55 | 5 | 1 | 133 | 1 | 116 | 2.00e-18 | 75.5 |
| MS.gene49158.t1 | AT5G52760 | 37.857 | 140 | 64 | 7 | 2 | 135 | 4 | 126 | 1.02e-16 | 71.2 |
| MS.gene49158.t1 | AT5G52750 | 46.154 | 65 | 35 | 0 | 3 | 67 | 5 | 69 | 1.75e-13 | 63.5 |
| MS.gene49158.t1 | AT5G52730 | 43.243 | 74 | 39 | 3 | 4 | 76 | 44 | 115 | 5.94e-11 | 57.8 |
Find 22 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGCAGTCTCTGGCCTTTC+AGG | 0.147290 | 1.3:-68680165 | MS.gene49158:intron |
| AGGATCCTCTTCAACGCTTA+TGG | 0.283035 | 1.3:+68679559 | None:intergenic |
| ATTAATCCTTTCATGCTCTA+TGG | 0.308553 | 1.3:-68679638 | MS.gene49158:CDS |
| TGGATCAATGTCTCCTATTA+AGG | 0.318014 | 1.3:+68679799 | None:intergenic |
| GACAAGAAAATGACCTTAAT+AGG | 0.340701 | 1.3:-68679812 | MS.gene49158:CDS |
| ATTCATCTGATGATTATAAT+AGG | 0.369814 | 1.3:+68679604 | None:intergenic |
| TAGATGATGACACAGCCATT+AGG | 0.441354 | 1.3:+68679539 | None:intergenic |
| ACAGAAAGTTGTGTTGAAGT+TGG | 0.461760 | 1.3:-68680223 | MS.gene49158:intron |
| TTCAGTTGGACCAGCTAAAG+AGG | 0.475094 | 1.3:-68679729 | MS.gene49158:CDS |
| AAAGCTATGAAAGCAGTCTC+TGG | 0.485853 | 1.3:-68680174 | MS.gene49158:CDS |
| AGCTTAGCCACTACACGTAC+TGG | 0.496210 | 1.3:+68679779 | None:intergenic |
| CATGCTGAAATACTTTCAGT+TGG | 0.503143 | 1.3:-68679743 | MS.gene49158:CDS |
| GCACGGTGGTATGAATTGTA+TGG | 0.520307 | 1.3:+68679581 | None:intergenic |
| TGATGATTATAATAGGTAGT+AGG | 0.521368 | 1.3:+68679611 | None:intergenic |
| CCGTGCCATAAGCGTTGAAG+AGG | 0.546888 | 1.3:-68679564 | MS.gene49158:CDS |
| GGTGTACCATAGAGCATGAA+AGG | 0.548293 | 1.3:+68679632 | None:intergenic |
| TACGTGTAGTGGCTAAGCTA+AGG | 0.568064 | 1.3:-68679775 | MS.gene49158:CDS |
| TAGTTAATTCAATACCTGAA+AGG | 0.578105 | 1.3:+68680151 | None:intergenic |
| AGCGTTGAAGAGGATCCTAA+TGG | 0.594188 | 1.3:-68679554 | MS.gene49158:CDS |
| CATTGATCCAGTACGTGTAG+TGG | 0.602340 | 1.3:-68679786 | MS.gene49158:CDS |
| CCTCTTCAACGCTTATGGCA+CGG | 0.607325 | 1.3:+68679564 | None:intergenic |
| CTTCAACGCTTATGGCACGG+TGG | 0.735189 | 1.3:+68679567 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAAAAGATGATAAAAAGA+AGG | - | chr1.3:68680272-68680291 | MS.gene49158:intron | 15.0% |
| !! | TTTAGATTAATAAAGTGTAT+TGG | - | chr1.3:68679958-68679977 | MS.gene49158:intron | 15.0% |
| !!! | TTCTTTTTATCATCTTTTTT+AGG | + | chr1.3:68680273-68680292 | None:intergenic | 15.0% |
| !! | ATTCATCTGATGATTATAAT+AGG | + | chr1.3:68680361-68680380 | None:intergenic | 20.0% |
| !! | TAAAATATCGAACTTGAAAT+CGG | + | chr1.3:68679942-68679961 | None:intergenic | 20.0% |
| !! | TAGAAATTGTTCAAAAGAAA+AGG | + | chr1.3:68679627-68679646 | None:intergenic | 20.0% |
| !!! | AAGTATTGATTCTATAGATT+TGG | + | chr1.3:68680046-68680065 | None:intergenic | 20.0% |
| ! | AAAATATCGAACTTGAAATC+GGG | + | chr1.3:68679941-68679960 | None:intergenic | 25.0% |
| ! | TAAAAGAGTGGTATTAATCA+AGG | + | chr1.3:68679566-68679585 | None:intergenic | 25.0% |
| ! | TAGTTAATTCAATACCTGAA+AGG | + | chr1.3:68679814-68679833 | None:intergenic | 25.0% |
| ! | TATCAATGCTAAAGCATAAT+TGG | - | chr1.3:68679593-68679612 | MS.gene49158:CDS | 25.0% |
| ! | TGATAAAGAAGATAAAAGAG+TGG | + | chr1.3:68679578-68679597 | None:intergenic | 25.0% |
| ! | TGATGATTATAATAGGTAGT+AGG | + | chr1.3:68680354-68680373 | None:intergenic | 25.0% |
| ! | TTGGAAATTGAAAATCTGAT+TGG | - | chr1.3:68679977-68679996 | MS.gene49158:intron | 25.0% |
| !! | TTGAATTTGAAGTAGTTCTT+TGG | - | chr1.3:68679649-68679668 | MS.gene49158:CDS | 25.0% |
| ATTAATCCTTTCATGCTCTA+TGG | - | chr1.3:68680324-68680343 | MS.gene49158:intron | 30.0% | |
| GACAAGAAAATGACCTTAAT+AGG | - | chr1.3:68680150-68680169 | MS.gene49158:intron | 30.0% | |
| GTATTTGCAATATTGTTGCA+GGG | - | chr1.3:68680100-68680119 | MS.gene49158:intron | 30.0% | |
| TATTTGCAATATTGTTGCAG+GGG | - | chr1.3:68680101-68680120 | MS.gene49158:intron | 30.0% | |
| TGTATTTGCAATATTGTTGC+AGG | - | chr1.3:68680099-68680118 | MS.gene49158:intron | 30.0% | |
| ACAGAAAGTTGTGTTGAAGT+TGG | - | chr1.3:68679739-68679758 | MS.gene49158:CDS | 35.0% | |
| CATGCTGAAATACTTTCAGT+TGG | - | chr1.3:68680219-68680238 | MS.gene49158:CDS | 35.0% | |
| TAGATGTGGATGATTGTGTA+TGG | - | chr1.3:68680006-68680025 | MS.gene49158:intron | 35.0% | |
| TGGATCAATGTCTCCTATTA+AGG | + | chr1.3:68680166-68680185 | None:intergenic | 35.0% | |
| ! | TCTTTCTTTTCCTCTTTAGC+TGG | + | chr1.3:68680246-68680265 | None:intergenic | 35.0% |
| AAAGCTATGAAAGCAGTCTC+TGG | - | chr1.3:68679788-68679807 | MS.gene49158:CDS | 40.0% | |
| CTGATTGGACAGTTTAGATG+TGG | - | chr1.3:68679992-68680011 | MS.gene49158:intron | 40.0% | |
| TAGATGATGACACAGCCATT+AGG | + | chr1.3:68680426-68680445 | None:intergenic | 40.0% | |
| AACATTTCAGACGTGACCGA+CGG | + | chr1.3:68679914-68679933 | None:intergenic | 45.0% | |
| AGGATCCTCTTCAACGCTTA+TGG | + | chr1.3:68680406-68680425 | None:intergenic | 45.0% | |
| ATCTCGTGACTCTATACCGT+CGG | - | chr1.3:68679895-68679914 | MS.gene49158:intron | 45.0% | |
| CATTGATCCAGTACGTGTAG+TGG | - | chr1.3:68680176-68680195 | MS.gene49158:CDS | 45.0% | |
| GCACGGTGGTATGAATTGTA+TGG | + | chr1.3:68680384-68680403 | None:intergenic | 45.0% | |
| GGTGTACCATAGAGCATGAA+AGG | + | chr1.3:68680333-68680352 | None:intergenic | 45.0% | |
| TACGTGTAGTGGCTAAGCTA+AGG | - | chr1.3:68680187-68680206 | MS.gene49158:CDS | 45.0% | |
| TTCAGTTGGACCAGCTAAAG+AGG | - | chr1.3:68680233-68680252 | MS.gene49158:intron | 45.0% | |
| ! | AGCGTTGAAGAGGATCCTAA+TGG | - | chr1.3:68680408-68680427 | MS.gene49158:intron | 45.0% |
| AGCTTAGCCACTACACGTAC+TGG | + | chr1.3:68680186-68680205 | None:intergenic | 50.0% | |
| CCTCTTCAACGCTTATGGCA+CGG | + | chr1.3:68680401-68680420 | None:intergenic | 50.0% | |
| ! | AAAGCAGTCTCTGGCCTTTC+AGG | - | chr1.3:68679797-68679816 | MS.gene49158:CDS | 50.0% |
| CTTCAACGCTTATGGCACGG+TGG | + | chr1.3:68680398-68680417 | None:intergenic | 55.0% | |
| !! | CCGTGCCATAAGCGTTGAAG+AGG | - | chr1.3:68680398-68680417 | MS.gene49158:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 68679538 | 68680446 | 68679538 | ID=MS.gene49158 |
| chr1.3 | mRNA | 68679538 | 68680446 | 68679538 | ID=MS.gene49158.t1;Parent=MS.gene49158 |
| chr1.3 | exon | 68680441 | 68680446 | 68680441 | ID=MS.gene49158.t1.exon1;Parent=MS.gene49158.t1 |
| chr1.3 | CDS | 68680441 | 68680446 | 68680441 | ID=cds.MS.gene49158.t1;Parent=MS.gene49158.t1 |
| chr1.3 | exon | 68680166 | 68680241 | 68680166 | ID=MS.gene49158.t1.exon2;Parent=MS.gene49158.t1 |
| chr1.3 | CDS | 68680166 | 68680241 | 68680166 | ID=cds.MS.gene49158.t1;Parent=MS.gene49158.t1 |
| chr1.3 | exon | 68679538 | 68679863 | 68679538 | ID=MS.gene49158.t1.exon3;Parent=MS.gene49158.t1 |
| chr1.3 | CDS | 68679538 | 68679863 | 68679538 | ID=cds.MS.gene49158.t1;Parent=MS.gene49158.t1 |
| Gene Sequence |
| Protein sequence |
