Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49284.t1 | XP_013468489.1 | 98.2 | 272 | 5 | 0 | 1 | 272 | 116 | 387 | 3.00E-152 | 547.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49284.t1 | Q9M0R2 | 46.7 | 261 | 132 | 3 | 16 | 272 | 326 | 583 | 1.6e-61 | 237.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49284.t1 | A0A072VLM7 | 98.2 | 272 | 5 | 0 | 1 | 272 | 116 | 387 | 2.1e-152 | 547.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049396 | MS.gene49284 | 0.832592 | 8.66E-56 | -1.69E-46 |
| MS.gene053318 | MS.gene49284 | 0.802537 | 5.35E-49 | -1.69E-46 |
| MS.gene053626 | MS.gene49284 | 0.816384 | 5.69E-52 | -1.69E-46 |
| MS.gene054003 | MS.gene49284 | 0.832414 | 9.58E-56 | -1.69E-46 |
| MS.gene055486 | MS.gene49284 | 0.81539 | 9.47E-52 | -1.69E-46 |
| MS.gene055519 | MS.gene49284 | 0.808232 | 3.43E-50 | -1.69E-46 |
| MS.gene055909 | MS.gene49284 | 0.812275 | 4.60E-51 | -1.69E-46 |
| MS.gene055911 | MS.gene49284 | 0.81206 | 5.13E-51 | -1.69E-46 |
| MS.gene05601 | MS.gene49284 | 0.806223 | 9.13E-50 | -1.69E-46 |
| MS.gene056166 | MS.gene49284 | 0.839169 | 1.86E-57 | -1.69E-46 |
| MS.gene056422 | MS.gene49284 | 0.821837 | 3.27E-53 | -1.69E-46 |
| MS.gene056448 | MS.gene49284 | 0.801269 | 9.75E-49 | -1.69E-46 |
| MS.gene057086 | MS.gene49284 | 0.826393 | 2.78E-54 | -1.69E-46 |
| MS.gene057734 | MS.gene49284 | 0.822741 | 2.01E-53 | -1.69E-46 |
| MS.gene059367 | MS.gene49284 | 0.817489 | 3.21E-52 | -1.69E-46 |
| MS.gene059368 | MS.gene49284 | 0.83324 | 5.98E-56 | -1.69E-46 |
| MS.gene060482 | MS.gene49284 | 0.800574 | 1.35E-48 | -1.69E-46 |
| MS.gene060837 | MS.gene49284 | 0.841119 | 5.77E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 47 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTATTGAGGAAATTCTTAA+AGG | 0.227165 | 1.1:+52036049 | MS.gene49284:CDS |
| ATCTTAGAGATATTACATTA+TGG | 0.248604 | 1.1:+52035982 | MS.gene49284:CDS |
| TAAAATTCTTGATGAAGATT+TGG | 0.250588 | 1.1:+52036148 | MS.gene49284:CDS |
| TGGTGTTGATGGAATTATTC+AGG | 0.274026 | 1.1:+52036367 | MS.gene49284:CDS |
| AGCTCTTTCATTCCTTGAAT+TGG | 0.286220 | 1.1:-52036410 | None:intergenic |
| TTGTAGTAAATGCACCATTT+TGG | 0.309402 | 1.1:+52036611 | MS.gene49284:CDS |
| AAAATTCTTGATGAAGATTT+GGG | 0.329182 | 1.1:+52036149 | MS.gene49284:CDS |
| GCTCAACTCCGTCAGCAAAA+AGG | 0.352272 | 1.1:+52036430 | MS.gene49284:CDS |
| AACACTCACCTGAGAAGAAT+TGG | 0.366039 | 1.1:-52036721 | None:intergenic |
| TCTTAGAGATATTACATTAT+GGG | 0.371202 | 1.1:+52035983 | MS.gene49284:CDS |
| TGTGCCCTACTTGGTAATAA+AGG | 0.371867 | 1.1:-52036011 | None:intergenic |
| GAGATGGAGGATTCAATTGA+TGG | 0.376525 | 1.1:+52036313 | MS.gene49284:CDS |
| AATTTGTACAAGAAGACATT+TGG | 0.376644 | 1.1:+52036263 | MS.gene49284:CDS |
| GAGCATCTTCCAGCTGGATA+TGG | 0.380972 | 1.1:+52036835 | MS.gene49284:CDS |
| CAGCTGGATATGGTGGTCTA+AGG | 0.408968 | 1.1:+52036845 | MS.gene49284:CDS |
| TATCCAGCTGGAAGATGCTC+TGG | 0.411235 | 1.1:-52036832 | None:intergenic |
| TTGAAAAGTGCACCAATTCA+AGG | 0.425968 | 1.1:+52036398 | MS.gene49284:CDS |
| TGTGAAGTGTAGAGCCAAAA+TGG | 0.434368 | 1.1:-52036625 | None:intergenic |
| TGTTACAGAAGACATTGGTT+TGG | 0.442089 | 1.1:+52036105 | MS.gene49284:CDS |
| TGCTCTAAAACTTTATCATC+AGG | 0.448465 | 1.1:-52036892 | None:intergenic |
| AGAATTGGTGTGACCCTTTG+TGG | 0.480253 | 1.1:-52036706 | None:intergenic |
| GGCCCAGAGCATCTTCCAGC+TGG | 0.482220 | 1.1:+52036829 | MS.gene49284:CDS |
| GTACCTTCATGTGCCCTACT+TGG | 0.493972 | 1.1:-52036020 | None:intergenic |
| ATATTTGGATAGTAGAGATA+AGG | 0.496144 | 1.1:+52036193 | MS.gene49284:CDS |
| TTGTTGAGCACCTCATTGAC+TGG | 0.499653 | 1.1:-52036952 | None:intergenic |
| GTGTTCCTTTATTACCAAGT+AGG | 0.502140 | 1.1:+52036006 | MS.gene49284:CDS |
| TGACCCTTTGTGGTCTTGCA+AGG | 0.502712 | 1.1:-52036696 | None:intergenic |
| TGTTCCTTTATTACCAAGTA+GGG | 0.503280 | 1.1:+52036007 | MS.gene49284:CDS |
| GGGTCACACCAATTCTTCTC+AGG | 0.506747 | 1.1:+52036713 | MS.gene49284:CDS |
| CATCCTTGCAAGACCACAAA+GGG | 0.520308 | 1.1:+52036693 | MS.gene49284:CDS |
| CTTCACAAGTGTTATTCTCA+AGG | 0.523067 | 1.1:+52036641 | MS.gene49284:CDS |
| ATGAAATGTCACAGGTACAT+AGG | 0.530202 | 1.1:+52036808 | MS.gene49284:intron |
| CTTAGAGATATTACATTATG+GGG | 0.542377 | 1.1:+52035984 | MS.gene49284:CDS |
| TCGTAAACATAGTAACAAAC+CGG | 0.557690 | 1.1:-52036224 | None:intergenic |
| TAGAGATAAGGAAGGTCGAC+CGG | 0.565192 | 1.1:+52036205 | MS.gene49284:CDS |
| CTTAGACCACCATATCCAGC+TGG | 0.567452 | 1.1:-52036844 | None:intergenic |
| CATCTTCCAGCTGGATATGG+TGG | 0.574311 | 1.1:+52036838 | MS.gene49284:CDS |
| ATCCAGCTGGAAGATGCTCT+GGG | 0.577545 | 1.1:-52036831 | None:intergenic |
| GTTGTTTCAAAGCTATTACC+CGG | 0.594800 | 1.1:+52036460 | MS.gene49284:CDS |
| TGACATGTTACAGAAGACAT+TGG | 0.594854 | 1.1:+52036100 | MS.gene49284:CDS |
| CTGGATATGGTGGTCTAAGG+AGG | 0.598272 | 1.1:+52036848 | MS.gene49284:CDS |
| AGTTGAATTTCCAGTCAATG+AGG | 0.619748 | 1.1:+52036942 | MS.gene49284:CDS |
| TTGGATAGTAGAGATAAGGA+AGG | 0.628322 | 1.1:+52036197 | MS.gene49284:CDS |
| TTACCAAGTAGGGCACATGA+AGG | 0.629064 | 1.1:+52036017 | MS.gene49284:CDS |
| AAGGTACTGATATTGTATTG+AGG | 0.652952 | 1.1:+52036036 | MS.gene49284:CDS |
| TCATCCTTGCAAGACCACAA+AGG | 0.655234 | 1.1:+52036692 | MS.gene49284:CDS |
| GGTTTGTTACTATGTTTACG+AGG | 0.673551 | 1.1:+52036226 | MS.gene49284:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTCTTGATGAAGATTT+GGG | + | chr1.1:52036149-52036168 | MS.gene49284:CDS | 20.0% |
| !! | ATCTTAGAGATATTACATTA+TGG | + | chr1.1:52035982-52036001 | MS.gene49284:CDS | 20.0% |
| !! | TAAAATTCTTGATGAAGATT+TGG | + | chr1.1:52036148-52036167 | MS.gene49284:CDS | 20.0% |
| !! | TCTTAGAGATATTACATTAT+GGG | + | chr1.1:52035983-52036002 | MS.gene49284:CDS | 20.0% |
| !! | TTCATATATACTATAAGCTA+TGG | - | chr1.1:52036578-52036597 | None:intergenic | 20.0% |
| !!! | ACATTTTTATAAACTATCTC+CGG | - | chr1.1:52036482-52036501 | None:intergenic | 20.0% |
| !!! | TTTTGAAAAAGTTGGATATT+TGG | + | chr1.1:52036178-52036197 | MS.gene49284:CDS | 20.0% |
| ! | AATTTGTACAAGAAGACATT+TGG | + | chr1.1:52036263-52036282 | MS.gene49284:CDS | 25.0% |
| ! | AGAAAAAATTTCATTGCCAA+AGG | + | chr1.1:52035956-52035975 | MS.gene49284:CDS | 25.0% |
| ! | ATATTTGGATAGTAGAGATA+AGG | + | chr1.1:52036193-52036212 | MS.gene49284:CDS | 25.0% |
| ! | CTTAGAGATATTACATTATG+GGG | + | chr1.1:52035984-52036003 | MS.gene49284:CDS | 25.0% |
| ! | TGTATTGAGGAAATTCTTAA+AGG | + | chr1.1:52036049-52036068 | MS.gene49284:CDS | 25.0% |
| ! | TTAACATTATGAAATGTCAC+AGG | + | chr1.1:52036800-52036819 | MS.gene49284:intron | 25.0% |
| !! | CATTTTTATAAACTATCTCC+GGG | - | chr1.1:52036481-52036500 | None:intergenic | 25.0% |
| !!! | ATATCTCTAAGATTTTCCTT+TGG | - | chr1.1:52035975-52035994 | None:intergenic | 25.0% |
| !!! | ATTAAGAAGTTGAGTTTTAG+AGG | + | chr1.1:52036344-52036363 | MS.gene49284:CDS | 25.0% |
| TATCATCAGGAGAAAAATCT+TGG | - | chr1.1:52036882-52036901 | None:intergenic | 30.0% | |
| TCGTAAACATAGTAACAAAC+CGG | - | chr1.1:52036227-52036246 | None:intergenic | 30.0% | |
| TGTTCCTTTATTACCAAGTA+GGG | + | chr1.1:52036007-52036026 | MS.gene49284:CDS | 30.0% | |
| ! | TTGTAGTAAATGCACCATTT+TGG | + | chr1.1:52036611-52036630 | MS.gene49284:CDS | 30.0% |
| !! | AAGGTACTGATATTGTATTG+AGG | + | chr1.1:52036036-52036055 | MS.gene49284:CDS | 30.0% |
| !! | GGTTCTGATTTTGAAAAAGT+TGG | + | chr1.1:52036170-52036189 | MS.gene49284:CDS | 30.0% |
| !! | TGAACTTCTTTTTGTTTCTC+TGG | - | chr1.1:52036675-52036694 | None:intergenic | 30.0% |
| !! | TGCTCTAAAACTTTATCATC+AGG | - | chr1.1:52036895-52036914 | None:intergenic | 30.0% |
| !!! | AATGTGACTTGTTTTTGAGA+TGG | + | chr1.1:52036297-52036316 | MS.gene49284:CDS | 30.0% |
| AGCTCTTTCATTCCTTGAAT+TGG | - | chr1.1:52036413-52036432 | None:intergenic | 35.0% | |
| AGTTGAATTTCCAGTCAATG+AGG | + | chr1.1:52036942-52036961 | MS.gene49284:CDS | 35.0% | |
| ATGAAATGTCACAGGTACAT+AGG | + | chr1.1:52036808-52036827 | MS.gene49284:intron | 35.0% | |
| GTGTTCCTTTATTACCAAGT+AGG | + | chr1.1:52036006-52036025 | MS.gene49284:CDS | 35.0% | |
| TGACATGTTACAGAAGACAT+TGG | + | chr1.1:52036100-52036119 | MS.gene49284:CDS | 35.0% | |
| TTGGATAGTAGAGATAAGGA+AGG | + | chr1.1:52036197-52036216 | MS.gene49284:CDS | 35.0% | |
| ! | CTTCACAAGTGTTATTCTCA+AGG | + | chr1.1:52036641-52036660 | MS.gene49284:CDS | 35.0% |
| ! | GTTGTTTCAAAGCTATTACC+CGG | + | chr1.1:52036460-52036479 | MS.gene49284:CDS | 35.0% |
| ! | TGTTACAGAAGACATTGGTT+TGG | + | chr1.1:52036105-52036124 | MS.gene49284:CDS | 35.0% |
| ! | TTGAAAAGTGCACCAATTCA+AGG | + | chr1.1:52036398-52036417 | MS.gene49284:CDS | 35.0% |
| !! | GGTTTGTTACTATGTTTACG+AGG | + | chr1.1:52036226-52036245 | MS.gene49284:CDS | 35.0% |
| !! | TGGTGTTGATGGAATTATTC+AGG | + | chr1.1:52036367-52036386 | MS.gene49284:CDS | 35.0% |
| !!! | AAGAAGTTGAGTTTTAGAGG+TGG | + | chr1.1:52036347-52036366 | MS.gene49284:CDS | 35.0% |
| AACACTCACCTGAGAAGAAT+TGG | - | chr1.1:52036724-52036743 | None:intergenic | 40.0% | |
| GAGATGGAGGATTCAATTGA+TGG | + | chr1.1:52036313-52036332 | MS.gene49284:CDS | 40.0% | |
| TGTGAAGTGTAGAGCCAAAA+TGG | - | chr1.1:52036628-52036647 | None:intergenic | 40.0% | |
| ! | CATAAGAGCCTTTTTGCTGA+CGG | - | chr1.1:52036441-52036460 | None:intergenic | 40.0% |
| ! | TGTGCCCTACTTGGTAATAA+AGG | - | chr1.1:52036014-52036033 | None:intergenic | 40.0% |
| !!! | AGTTTTAGAGGTGGTGTTGA+TGG | + | chr1.1:52036356-52036375 | MS.gene49284:CDS | 40.0% |
| !!! | GTGACTTGTTTTTGAGATGG+AGG | + | chr1.1:52036300-52036319 | MS.gene49284:CDS | 40.0% |
| AGAATTGGTGTGACCCTTTG+TGG | - | chr1.1:52036709-52036728 | None:intergenic | 45.0% | |
| CATCCTTGCAAGACCACAAA+GGG | + | chr1.1:52036693-52036712 | MS.gene49284:CDS | 45.0% | |
| TAGAGATAAGGAAGGTCGAC+CGG | + | chr1.1:52036205-52036224 | MS.gene49284:CDS | 45.0% | |
| TCATCCTTGCAAGACCACAA+AGG | + | chr1.1:52036692-52036711 | MS.gene49284:CDS | 45.0% | |
| TTACCAAGTAGGGCACATGA+AGG | + | chr1.1:52036017-52036036 | MS.gene49284:CDS | 45.0% | |
| ATCCAGCTGGAAGATGCTCT+GGG | - | chr1.1:52036834-52036853 | None:intergenic | 50.0% | |
| CAGCTGGATATGGTGGTCTA+AGG | + | chr1.1:52036845-52036864 | MS.gene49284:CDS | 50.0% | |
| CATCTTCCAGCTGGATATGG+TGG | + | chr1.1:52036838-52036857 | MS.gene49284:CDS | 50.0% | |
| CTGGATATGGTGGTCTAAGG+AGG | + | chr1.1:52036848-52036867 | MS.gene49284:CDS | 50.0% | |
| CTTAGACCACCATATCCAGC+TGG | - | chr1.1:52036847-52036866 | None:intergenic | 50.0% | |
| GAGCATCTTCCAGCTGGATA+TGG | + | chr1.1:52036835-52036854 | MS.gene49284:CDS | 50.0% | |
| GCTCAACTCCGTCAGCAAAA+AGG | + | chr1.1:52036430-52036449 | MS.gene49284:CDS | 50.0% | |
| GGGTCACACCAATTCTTCTC+AGG | + | chr1.1:52036713-52036732 | MS.gene49284:CDS | 50.0% | |
| GTACCTTCATGTGCCCTACT+TGG | - | chr1.1:52036023-52036042 | None:intergenic | 50.0% | |
| TATCCAGCTGGAAGATGCTC+TGG | - | chr1.1:52036835-52036854 | None:intergenic | 50.0% | |
| ! | TGACCCTTTGTGGTCTTGCA+AGG | - | chr1.1:52036699-52036718 | None:intergenic | 50.0% |
| ! | GGCCCAGAGCATCTTCCAGC+TGG | + | chr1.1:52036829-52036848 | MS.gene49284:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 52035949 | 52036963 | 52035949 | ID=MS.gene49284 |
| chr1.1 | mRNA | 52035949 | 52036963 | 52035949 | ID=MS.gene49284.t1;Parent=MS.gene49284 |
| chr1.1 | exon | 52035949 | 52036499 | 52035949 | ID=MS.gene49284.t1.exon1;Parent=MS.gene49284.t1 |
| chr1.1 | CDS | 52035949 | 52036499 | 52035949 | ID=cds.MS.gene49284.t1;Parent=MS.gene49284.t1 |
| chr1.1 | exon | 52036610 | 52036734 | 52036610 | ID=MS.gene49284.t1.exon2;Parent=MS.gene49284.t1 |
| chr1.1 | CDS | 52036610 | 52036734 | 52036610 | ID=cds.MS.gene49284.t1;Parent=MS.gene49284.t1 |
| chr1.1 | exon | 52036822 | 52036963 | 52036822 | ID=MS.gene49284.t1.exon3;Parent=MS.gene49284.t1 |
| chr1.1 | CDS | 52036822 | 52036963 | 52036822 | ID=cds.MS.gene49284.t1;Parent=MS.gene49284.t1 |
| Gene Sequence |
| Protein sequence |
