AlfalfaGEDB | MS.gene49610

M. sativa cultivar XinJiangDaYe / MS.gene49610

KEGG: K03671
GO: GO:0003674 GO:0003824 GO:0006662 GO:0008150 GO:0008152 GO:0009987 GO:0015035 GO:0015036 GO:0016491 GO:0016667 GO:0018904 GO:0019725 GO:0042592 GO:0044281 GO:0045454 GO:0050789 GO:0050794 GO:0065007 GO:0065008 GO:0071704
PFAM: PF00085.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49610.t1	XP_003613544.1	94.4	126	7	0	1	126	1	126	1.10E-61	245.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49610.t1	Q38879	45.0	111	61	0	15	125	22	132	6.9e-28	124.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49610.t1	D8L5E0	94.4	126	7	0	1	126	1	126	8.0e-62	245.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049378	MS.gene49610	0.830888	2.28E-55	-1.69E-46
MS.gene050763	MS.gene49610	0.800293	1.54E-48	-1.69E-46
MS.gene050971	MS.gene49610	0.813742	2.19E-51	-1.69E-46
MS.gene052077	MS.gene49610	0.823134	1.63E-53	-1.69E-46
MS.gene05316	MS.gene49610	0.831238	1.87E-55	-1.69E-46
MS.gene053577	MS.gene49610	0.806272	8.92E-50	-1.69E-46
MS.gene054379	MS.gene49610	0.80212	6.52E-49	-1.69E-46
MS.gene054826	MS.gene49610	0.817496	3.20E-52	-1.69E-46
MS.gene054930	MS.gene49610	0.812575	3.96E-51	-1.69E-46
MS.gene057609	MS.gene49610	0.911416	6.23E-83	-1.69E-46
MS.gene057610	MS.gene49610	0.921985	1.77E-88	-1.69E-46
MS.gene058651	MS.gene49610	0.803583	3.25E-49	-1.69E-46
MS.gene058949	MS.gene49610	0.80937	1.96E-50	-1.69E-46
MS.gene059807	MS.gene49610	0.811235	7.75E-51	-1.69E-46
MS.gene059876	MS.gene49610	0.813064	3.09E-51	-1.69E-46
MS.gene060495	MS.gene49610	0.81357	2.39E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49610.t1	MTR_5g037890	94.444	126	7	0	1	126	1	126	6.32e-80	231
MS.gene49610.t1	MTR_5g037950	62.698	126	46	1	1	125	1	126	2.38e-49	154
MS.gene49610.t1	MTR_5g037930	62.500	128	46	1	1	126	1	128	4.02e-49	153
MS.gene49610.t1	MTR_7g009070	51.887	106	51	0	20	125	28	133	2.76e-40	131
MS.gene49610.t1	MTR_3g112410	44.144	111	62	0	14	124	2	112	4.19e-34	115
MS.gene49610.t1	MTR_5g021180	42.342	111	64	0	14	124	4	114	6.79e-33	112
MS.gene49610.t1	MTR_1g023140	39.823	113	68	0	14	126	2	114	2.96e-32	110
MS.gene49610.t1	MTR_8g107460	39.423	104	63	0	18	121	254	357	8.87e-23	91.7
MS.gene49610.t1	MTR_8g005320	32.381	105	71	0	17	121	25	129	2.47e-21	83.2
MS.gene49610.t1	MTR_7g073680	33.333	105	70	0	17	121	23	127	5.27e-21	82.4
MS.gene49610.t1	MTR_2g010750	37.143	105	66	0	18	122	9	113	5.60e-21	82.0
MS.gene49610.t1	MTR_8g006685	32.381	105	71	0	17	121	94	198	3.39e-20	82.0
MS.gene49610.t1	MTR_8g006560	32.381	105	71	0	17	121	94	198	3.39e-20	82.0
MS.gene49610.t1	MTR_4g081380	35.514	107	65	2	18	122	10	114	1.49e-19	78.2
MS.gene49610.t1	MTR_2g082590	30.476	105	73	0	18	122	10	114	4.51e-18	74.3
MS.gene49610.t1	MTR_2g082590	30.973	113	69	2	10	122	24	127	2.83e-17	72.8
MS.gene49610.t1	MTR_8g059015	34.091	88	56	2	38	123	87	174	2.75e-14	65.9
MS.gene49610.t1	MTR_3g089065	27.586	116	79	3	10	123	59	171	4.72e-14	65.5
MS.gene49610.t1	MTR_2g079420	34.091	88	56	2	38	123	84	171	1.84e-13	63.5
MS.gene49610.t1	MTR_4g094690	27.619	105	74	1	21	123	62	166	1.91e-13	63.5
MS.gene49610.t1	MTR_1g098660	29.070	86	58	2	39	123	77	160	6.76e-13	62.0
MS.gene49610.t1	MTR_7g080250	34.091	88	57	1	40	126	94	181	1.19e-12	61.6
MS.gene49610.t1	MTR_1g114290	32.911	79	49	2	38	115	84	159	4.67e-12	60.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49610.t1	AT5G39950	45.045	111	61	0	15	125	22	132	4.35e-39	128
MS.gene49610.t1	AT1G69880	47.664	107	56	0	17	123	36	142	4.37e-35	118
MS.gene49610.t1	AT1G59730	40.541	111	66	0	15	125	18	128	2.09e-32	111
MS.gene49610.t1	AT3G51030	40.909	110	65	0	14	123	2	111	1.36e-31	108
MS.gene49610.t1	AT1G45145	43.925	107	60	0	18	124	5	111	3.03e-31	107
MS.gene49610.t1	AT1G19730	42.982	114	64	1	14	126	2	115	2.20e-30	105
MS.gene49610.t1	AT5G42980	43.119	109	62	0	18	126	5	113	8.07e-30	104
MS.gene49610.t1	AT3G56420	36.957	92	58	0	28	119	50	141	8.43e-21	82.4
MS.gene49610.t1	AT3G56420	36.957	92	58	0	28	119	50	141	8.43e-21	82.4
MS.gene49610.t1	AT3G56420	36.957	92	58	0	28	119	50	141	8.43e-21	82.4
MS.gene49610.t1	AT3G17880	36.893	103	65	0	19	121	264	366	3.05e-20	84.7
MS.gene49610.t1	AT3G17880	36.893	103	65	0	19	121	271	373	3.57e-20	84.7
MS.gene49610.t1	AT3G08710	33.333	93	62	0	29	121	34	126	1.55e-18	76.3
MS.gene49610.t1	AT3G08710	33.333	93	62	0	29	121	34	126	1.55e-18	76.3
MS.gene49610.t1	AT3G08710	33.333	93	62	0	29	121	34	126	1.55e-18	76.3
MS.gene49610.t1	AT1G11530	32.381	105	71	0	18	122	2	106	1.36e-17	73.2
MS.gene49610.t1	AT2G40790	34.783	92	60	0	28	119	50	141	7.83e-17	72.0
MS.gene49610.t1	AT2G40790	34.783	92	60	0	28	119	50	141	7.83e-17	72.0
MS.gene49610.t1	AT4G03520	39.759	83	48	2	43	123	101	183	2.69e-15	68.9
MS.gene49610.t1	AT1G03680	39.506	81	47	2	45	123	97	177	6.69e-15	67.8
MS.gene49610.t1	AT3G15360	32.967	91	53	3	38	123	102	189	1.00e-14	67.4
MS.gene49610.t1	AT1G43560	26.496	117	81	2	1	112	33	149	2.59e-14	65.9
MS.gene49610.t1	AT5G16400	35.227	88	56	1	40	126	97	184	6.48e-14	65.1
MS.gene49610.t1	AT1G31020	30.833	120	77	2	6	123	41	156	7.62e-14	64.3
MS.gene49610.t1	AT3G02730	36.782	87	54	1	41	126	88	174	1.24e-13	64.3
MS.gene49610.t1	AT2G35010	34.444	90	55	2	37	123	103	191	5.44e-13	62.8
MS.gene49610.t1	AT2G35010	34.444	90	55	2	37	123	103	191	5.44e-13	62.8
MS.gene49610.t1	AT1G76760	27.368	95	62	3	33	120	70	164	9.81e-13	62.0
MS.gene49610.t1	AT1G52990	31.868	91	60	2	34	123	222	311	1.38e-11	60.5

Find 23 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATACCAGTTTATTAGTTTCT+TGG	0.217604	5.1:+28568012	None:intergenic
CATTCCATTCCACTGCTAAA+TGG	0.236688	5.1:-28568055	MS.gene49610:CDS
GCAGCAAAATCTTTCATAAC+TGG	0.263663	5.1:+28567853	None:intergenic
GCTTGATGTTGATGAGTTAT+TGG	0.264813	5.1:-28567803	MS.gene49610:intron
TTATTGATTTCACAGCTAAA+TGG	0.322109	5.1:-28567900	MS.gene49610:CDS
TTTCCAAGAAACTAATAAAC+TGG	0.393712	5.1:-28568015	MS.gene49610:intron
TTCTTAATGAGTAAAATAGT+TGG	0.439170	5.1:+28567552	None:intergenic
TAAAGTGGTTGGAGTGAGAA+AGG	0.465747	5.1:-28567514	MS.gene49610:CDS
GGGAGTTTCTCAAACATTCC+AGG	0.482800	5.1:-28567586	MS.gene49610:CDS
GTGTGGACCTTGCAAAGTTA+TGG	0.487071	5.1:-28567878	MS.gene49610:CDS
AGGAAATGTTGTTGATAAAG+TGG	0.502411	5.1:-28567529	MS.gene49610:CDS
AATGTTGTTGATAAAGTGGT+TGG	0.520853	5.1:-28567525	MS.gene49610:CDS
ATACACAAAATTATACAAGT+TGG	0.527315	5.1:+28568112	None:intergenic
CATTCCATTTAGCAGTGGAA+TGG	0.530623	5.1:+28568051	None:intergenic
TGGAATGGAATGCAAGGACA+TGG	0.540723	5.1:+28568066	None:intergenic
GATTTCACAGCTAAATGGTG+TGG	0.590907	5.1:-28567895	MS.gene49610:CDS
TAGTTGGCAATGAATGCACC+TGG	0.594643	5.1:+28567568	None:intergenic
ACTGGATCCATAACTTTGCA+AGG	0.602265	5.1:+28567871	None:intergenic
AGTGGTTGGAGTGAGAAAGG+AGG	0.605043	5.1:-28567511	MS.gene49610:CDS
ATGAGCATTCCATTTAGCAG+TGG	0.609708	5.1:+28568046	None:intergenic
TAGCAGTGGAATGGAATGCA+AGG	0.615008	5.1:+28568060	None:intergenic
TGAGAAAGGAGGAACTACAA+AGG	0.675876	5.1:-28567500	MS.gene49610:CDS
GGAATGGAATGCAAGGACAT+GGG	0.705076	5.1:+28568067	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAATAATAGCATTTATAAT+AGG	+	chr5.1:28567927-28567946	None:intergenic	10.0%
!!	ATACACAAAATTATACAAGT+TGG	+	chr5.1:28567486-28567505	None:intergenic	20.0%
!!	TTCTTAATGAGTAAAATAGT+TGG	+	chr5.1:28568046-28568065	None:intergenic	20.0%
!!!	ACTATTTTACTCATTAAGAA+AGG	-	chr5.1:28568046-28568065	MS.gene49610:CDS	20.0%
!!!	TCTTATGTAGTATTTTTCTT+TGG	-	chr5.1:28567663-28567682	MS.gene49610:intron	20.0%
!!!	TGTTTTTTCCACAATATAAA+TGG	-	chr5.1:28567981-28568000	MS.gene49610:intron	20.0%
!	TAACAAACTAGTTAAAGTGA+AGG	+	chr5.1:28567833-28567852	None:intergenic	25.0%
!	TTTCCAAGAAACTAATAAAC+TGG	-	chr5.1:28567580-28567599	MS.gene49610:CDS	25.0%
!!	ATACCAGTTTATTAGTTTCT+TGG	+	chr5.1:28567586-28567605	None:intergenic	25.0%
!!	GTATTTTTCTTTGGTATAGA+TGG	-	chr5.1:28567672-28567691	MS.gene49610:intron	25.0%
!!!	TTATTGATTTCACAGCTAAA+TGG	-	chr5.1:28567695-28567714	MS.gene49610:intron	25.0%
	AATTAAACCTGGCATAAAGT+TGG	+	chr5.1:28567867-28567886	None:intergenic	30.0%
!	AATGTTGTTGATAAAGTGGT+TGG	-	chr5.1:28568070-28568089	MS.gene49610:CDS	30.0%
!	AGGAAATGTTGTTGATAAAG+TGG	-	chr5.1:28568066-28568085	MS.gene49610:CDS	30.0%
	ACCAACTGAGTAATTAAACC+TGG	+	chr5.1:28567878-28567897	None:intergenic	35.0%
	CCACAATATAAATGGCAACA+GGG	-	chr5.1:28567989-28568008	MS.gene49610:intron	35.0%
	GCAGCAAAATCTTTCATAAC+TGG	+	chr5.1:28567745-28567764	None:intergenic	35.0%
	TCCACAATATAAATGGCAAC+AGG	-	chr5.1:28567988-28568007	MS.gene49610:intron	35.0%
	TGTAAAACCAACTTTATGCC+AGG	-	chr5.1:28567857-28567876	MS.gene49610:CDS	35.0%
!	GCTTGATGTTGATGAGTTAT+TGG	-	chr5.1:28567792-28567811	MS.gene49610:intron	35.0%
	ACTGGATCCATAACTTTGCA+AGG	+	chr5.1:28567727-28567746	None:intergenic	40.0%
	ATGAGCATTCCATTTAGCAG+TGG	+	chr5.1:28567552-28567571	None:intergenic	40.0%
	CATTCCATTCCACTGCTAAA+TGG	-	chr5.1:28567540-28567559	MS.gene49610:CDS	40.0%
	CATTCCATTTAGCAGTGGAA+TGG	+	chr5.1:28567547-28567566	None:intergenic	40.0%
	GATTTCACAGCTAAATGGTG+TGG	-	chr5.1:28567700-28567719	MS.gene49610:intron	40.0%
	GCCAGGTTTAATTACTCAGT+TGG	-	chr5.1:28567874-28567893	MS.gene49610:CDS	40.0%
	TAAAGTGGTTGGAGTGAGAA+AGG	-	chr5.1:28568081-28568100	MS.gene49610:CDS	40.0%
	TGAGAAAGGAGGAACTACAA+AGG	-	chr5.1:28568095-28568114	MS.gene49610:CDS	40.0%
!	CCCTGTTGCCATTTATATTG+TGG	+	chr5.1:28567992-28568011	None:intergenic	40.0%
	GGAATGGAATGCAAGGACAT+GGG	+	chr5.1:28567531-28567550	None:intergenic	45.0%
	GGGAGTTTCTCAAACATTCC+AGG	-	chr5.1:28568009-28568028	MS.gene49610:intron	45.0%
	GTGTGGACCTTGCAAAGTTA+TGG	-	chr5.1:28567717-28567736	MS.gene49610:intron	45.0%
	TAGCAGTGGAATGGAATGCA+AGG	+	chr5.1:28567538-28567557	None:intergenic	45.0%
	TAGTTGGCAATGAATGCACC+TGG	+	chr5.1:28568030-28568049	None:intergenic	45.0%
	TGGAATGGAATGCAAGGACA+TGG	+	chr5.1:28567532-28567551	None:intergenic	45.0%
	AGTGGTTGGAGTGAGAAAGG+AGG	-	chr5.1:28568084-28568103	MS.gene49610:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	28567476	28568141	28567476	ID=MS.gene49610
chr5.1	mRNA	28567476	28568141	28567476	ID=MS.gene49610.t1;Parent=MS.gene49610
chr5.1	exon	28568016	28568141	28568016	ID=MS.gene49610.t1.exon1;Parent=MS.gene49610.t1
chr5.1	CDS	28568016	28568141	28568016	ID=cds.MS.gene49610.t1;Parent=MS.gene49610.t1
chr5.1	exon	28567804	28567926	28567804	ID=MS.gene49610.t1.exon2;Parent=MS.gene49610.t1
chr5.1	CDS	28567804	28567926	28567804	ID=cds.MS.gene49610.t1;Parent=MS.gene49610.t1
chr5.1	exon	28567476	28567607	28567476	ID=MS.gene49610.t1.exon3;Parent=MS.gene49610.t1
chr5.1	CDS	28567476	28567607	28567476	ID=cds.MS.gene49610.t1;Parent=MS.gene49610.t1

Gene Sequence

>MS.gene49610

ATGAGTTCCAACTTGTATAATTTTGTGTATGTTGATAGATCAACAGAATCATCCCATGTCCTTGCATTCCATTCCACTGCTAAATGGAATGCTCATTTTGAAGCTTTCCAAGAAACTAATAAACTGGTATGTTTGCGTAGCTAAAATCTTAACTAGCTATACAATCTTAGTATATAAAATTTAGTTTTCTTATGTAGTATTTTTCTTTGGTATAGATGGTTATTGATTTCACAGCTAAATGGTGTGGACCTTGCAAAGTTATGGATCCAGTTATGAAAGATTTTGCTGCAAAATACACTGATGTTGAGTTCATTAAGCTTGATGTTGATGAGTTATTGGTATGTTACAGATTATCCTTCACTTTAACTAGTTTGTTATTTATGTAAAACCAACTTTATGCCAGGTTTAATTACTCAGTTGGTTTCTAAACTATTTGAATTTTTTTGAACCTATTATAAATGCTATTATTTTTTAGAAACATGTTTTCAACACTTGAGAACTGATTTGTTTTTTCCACAATATAAATGGCAACAGGGAGTTTCTCAAACATTCCAGGTGCATTCATTGCCAACTATTTTACTCATTAAGAAAGGAAATGTTGTTGATAAAGTGGTTGGAGTGAGAAAGGAGGAACTACAAAGGATGATTGAAAAACAAAGAAACTAA

Protein sequence

>MS.gene49610.t1

MSSNLYNFVYVDRSTESSHVLAFHSTAKWNAHFEAFQETNKLMVIDFTAKWCGPCKVMDPVMKDFAAKYTDVEFIKLDVDELLGVSQTFQVHSLPTILLIKKGNVVDKVVGVRKEELQRMIEKQRN