AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene49655


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49655.t1 MTR_5g037300 96.565 262 9 0 1 262 1 262 0.0 525
MS.gene49655.t1 MTR_8g061960 54.610 282 97 8 1 262 1 271 3.12e-79 251
MS.gene49655.t1 MTR_4g107360 41.870 246 108 6 18 262 23 234 1.51e-50 175
MS.gene49655.t1 MTR_2g018770 50.867 173 83 2 88 260 135 305 7.79e-48 167
MS.gene49655.t1 MTR_2g018770 50.867 173 83 2 88 260 135 305 4.12e-47 167
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49655.t1 AT5G62620 47.440 293 108 7 5 262 14 295 5.04e-74 237
MS.gene49655.t1 AT5G62620 47.099 293 109 7 5 262 14 295 3.70e-73 236
MS.gene49655.t1 AT1G74800 42.230 296 114 8 5 262 14 290 1.05e-60 203
MS.gene49655.t1 AT1G27120 43.985 266 100 7 32 261 1 253 1.24e-55 189
MS.gene49655.t1 AT1G27120 43.098 297 120 7 1 261 1 284 1.42e-55 189
MS.gene49655.t1 AT4G21060 35.000 300 154 9 1 261 1 298 4.69e-46 164
MS.gene49655.t1 AT4G21060 42.564 195 108 3 69 261 163 355 3.39e-44 159

Find 59 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAGTTCAGTTTCAGTTTC+TGG 0.041671 5.1:+27955407 MS.gene49655:CDS
GCAAAACATGCTTACATTTC+TGG 0.132073 5.1:+27955293 MS.gene49655:CDS
AGTTTCTGGGTTTGAGTTTA+GGG 0.201347 5.1:+27955421 MS.gene49655:CDS
CAGTTTCTGGGTTTGAGTTT+AGG 0.201904 5.1:+27955420 MS.gene49655:CDS
ACAGTTTATGTTGGAGTTGC+AGG 0.247501 5.1:+27955589 MS.gene49655:CDS
CACCGACGCAATGCCACTTT+TGG 0.259091 5.1:+27955163 MS.gene49655:CDS
TTGAACAGAATACTTGTTAT+AGG 0.265205 5.1:+27955699 MS.gene49655:CDS
TCAGAAACTGTAGCTAATAT+TGG 0.295572 5.1:+27955131 MS.gene49655:CDS
CAGAAACTGTAGCTAATATT+GGG 0.295921 5.1:+27955132 MS.gene49655:CDS
GGAGAAGTTGAAAGGGGTTA+TGG 0.303614 5.1:+27955442 MS.gene49655:CDS
CAAGTATTCTGTTCAATAAC+AGG 0.310820 5.1:-27955692 None:intergenic
GGATTAAAATGCAATATCTT+TGG 0.314681 5.1:-27955641 None:intergenic
TGAGTTCAGTTTCAGTTTCT+GGG 0.315691 5.1:+27955408 MS.gene49655:CDS
AAATGGTGATGAAAAGGTTT+TGG 0.346138 5.1:+27955562 MS.gene49655:CDS
AGCTTTAACAATAGCTTTGA+AGG 0.353027 5.1:+27955248 MS.gene49655:CDS
TGGTCTCATGTCACTGTTGT+GGG 0.372257 5.1:+27955488 MS.gene49655:CDS
TGAATCAGTGTGAGTCTGTT+TGG 0.386247 5.1:-27955029 None:intergenic
TTAGCTTTCAAAGCAGGGTT+AGG 0.403086 5.1:+27955107 MS.gene49655:CDS
ATCCTAGGCTTAAAGGGGAT+TGG 0.404483 5.1:+27955660 MS.gene49655:CDS
GAATCAGTGTGAGTCTGTTT+GGG 0.411570 5.1:-27955028 None:intergenic
TCTCGCGCAGACGAGGAAAC+TGG 0.429538 5.1:+27955761 MS.gene49655:CDS
TTTGGTTTCACAGTTTATGT+TGG 0.434741 5.1:+27955580 MS.gene49655:CDS
AGTTTAGGGAGAAGTTGAAA+GGG 0.446263 5.1:+27955435 MS.gene49655:CDS
AGCCGAAAATGGTTCTGATT+CGG 0.454611 5.1:+27955376 MS.gene49655:CDS
TGAATTGTGGGTTGACACTT+TGG 0.462030 5.1:+27955468 MS.gene49655:CDS
ATTCCCTTAGCTTTCAAAGC+AGG 0.463054 5.1:+27955101 MS.gene49655:CDS
TTCTGTAATAATAGCCCAAA+GGG 0.467532 5.1:-27955212 None:intergenic
AAAGCTATTGTTAAAGCTCA+AGG 0.468037 5.1:-27955243 None:intergenic
TACTTGTTATAGGATGCAAT+GGG 0.469999 5.1:+27955709 MS.gene49655:CDS
CAGTTTATGTTGGAGTTGCA+GGG 0.480007 5.1:+27955590 MS.gene49655:CDS
TTCCCTTAGCTTTCAAAGCA+GGG 0.485510 5.1:+27955102 MS.gene49655:CDS
GAGTTTAGGGAGAAGTTGAA+AGG 0.487616 5.1:+27955434 MS.gene49655:CDS
TTAAAATGCAATATCTTTGG+TGG 0.500868 5.1:-27955638 None:intergenic
CTCCAATCCCCTTTAAGCCT+AGG 0.511635 5.1:-27955662 None:intergenic
CAAAAGTGGCATTGCGTCGG+TGG 0.518507 5.1:-27955162 None:intergenic
TAACCCTGCTTTGAAAGCTA+AGG 0.531599 5.1:-27955105 None:intergenic
TTGGTCTCATGTCACTGTTG+TGG 0.531891 5.1:+27955487 MS.gene49655:CDS
GATCATACATGCATATCAAA+GGG 0.534974 5.1:-27955795 None:intergenic
GTTTAGGGAGAAGTTGAAAG+GGG 0.540195 5.1:+27955436 MS.gene49655:CDS
ATACTTGTTATAGGATGCAA+TGG 0.559411 5.1:+27955708 MS.gene49655:CDS
TTCCAAAAGTGGCATTGCGT+CGG 0.559633 5.1:-27955165 None:intergenic
CTGGTCTTAGGTGTGAGGGA+TGG 0.564643 5.1:+27955735 MS.gene49655:CDS
TTATGGGAAGAAGTTGAAAG+TGG 0.572071 5.1:+27955323 MS.gene49655:CDS
GGGACTGGTCTTAGGTGTGA+GGG 0.573659 5.1:+27955731 MS.gene49655:CDS
GGTCTCATGTCACTGTTGTG+GGG 0.579148 5.1:+27955489 MS.gene49655:CDS
GGTGAGAGTGATCCAAAGAT+TGG 0.581545 5.1:+27955530 MS.gene49655:CDS
AACCCTGCTTTGAAAGCTAA+GGG 0.581962 5.1:-27955104 None:intergenic
AAGCAGAACAAAGATGAAGA+AGG 0.595481 5.1:+27954983 None:intergenic
AGAAACTGTAGCTAATATTG+GGG 0.603844 5.1:+27955133 MS.gene49655:CDS
ATGGAAGTCTCGCGCAGACG+AGG 0.612244 5.1:+27955754 MS.gene49655:CDS
TTTCTGTAATAATAGCCCAA+AGG 0.619004 5.1:-27955213 None:intergenic
GGGGACTGGTCTTAGGTGTG+AGG 0.628385 5.1:+27955730 MS.gene49655:CDS
AGGCTTAAAGGGGATTGGAG+TGG 0.637414 5.1:+27955665 MS.gene49655:CDS
CTCTCACCATGTGCATAACG+TGG 0.646342 5.1:-27955515 None:intergenic
CTTGTTATAGGATGCAATGG+GGG 0.660917 5.1:+27955711 MS.gene49655:CDS
GAGAGTGATCCAAAGATTGG+TGG 0.662396 5.1:+27955533 MS.gene49655:CDS
AGGTGTATCAGAGCTTGACA+AGG 0.692114 5.1:+27955268 MS.gene49655:CDS
GGGACACCACGTTATGCACA+TGG 0.708767 5.1:+27955509 MS.gene49655:CDS
ACTTGTTATAGGATGCAATG+GGG 0.716500 5.1:+27955710 MS.gene49655:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GGATTAAAATGCAATATCTT+TGG - chr5.1:27955644-27955663 None:intergenic 25.0%
! TTAAAATGCAATATCTTTGG+TGG - chr5.1:27955641-27955660 None:intergenic 25.0%
!! TGGTAAAGTTAAGAGTTTTT+CGG + chr5.1:27955343-27955362 MS.gene49655:CDS 25.0%
!!! ACATTTCTGGTAAAAAGTTA+TGG + chr5.1:27955306-27955325 MS.gene49655:CDS 25.0%
!!! AGATATTGCATTTTAATCCT+AGG + chr5.1:27955645-27955664 MS.gene49655:CDS 25.0%
!!! ATTTTAATCCTAGGCTTAAA+GGG + chr5.1:27955654-27955673 MS.gene49655:CDS 25.0%
!!! CATTTCTGGTAAAAAGTTAT+GGG + chr5.1:27955307-27955326 MS.gene49655:CDS 25.0%
!!! TTGAACAGAATACTTGTTAT+AGG + chr5.1:27955699-27955718 MS.gene49655:CDS 25.0%
AAAGCTATTGTTAAAGCTCA+AGG - chr5.1:27955246-27955265 None:intergenic 30.0%
AGAAACTGTAGCTAATATTG+GGG + chr5.1:27955133-27955152 MS.gene49655:CDS 30.0%
CAAGTATTCTGTTCAATAAC+AGG - chr5.1:27955695-27955714 None:intergenic 30.0%
CAGAAACTGTAGCTAATATT+GGG + chr5.1:27955132-27955151 MS.gene49655:CDS 30.0%
TCAGAAACTGTAGCTAATAT+TGG + chr5.1:27955131-27955150 MS.gene49655:CDS 30.0%
TTCTGTAATAATAGCCCAAA+GGG - chr5.1:27955215-27955234 None:intergenic 30.0%
TTTCTGTAATAATAGCCCAA+AGG - chr5.1:27955216-27955235 None:intergenic 30.0%
! GGTAAAGTTAAGAGTTTTTC+GGG + chr5.1:27955344-27955363 MS.gene49655:CDS 30.0%
! TGTGAAAAATGGTGATGAAA+AGG + chr5.1:27955556-27955575 MS.gene49655:CDS 30.0%
! TTTGGTTTCACAGTTTATGT+TGG + chr5.1:27955580-27955599 MS.gene49655:CDS 30.0%
! TTTTAATCCTAGGCTTAAAG+GGG + chr5.1:27955655-27955674 MS.gene49655:CDS 30.0%
!! AAATGGTGATGAAAAGGTTT+TGG + chr5.1:27955562-27955581 MS.gene49655:CDS 30.0%
!! AGCTTTAACAATAGCTTTGA+AGG + chr5.1:27955248-27955267 MS.gene49655:CDS 30.0%
!! ATACTTGTTATAGGATGCAA+TGG + chr5.1:27955708-27955727 MS.gene49655:CDS 30.0%
!! CATTTTAATCCTAGGCTTAA+AGG + chr5.1:27955653-27955672 MS.gene49655:CDS 30.0%
!! TACTTGTTATAGGATGCAAT+GGG + chr5.1:27955709-27955728 MS.gene49655:CDS 30.0%
!!! GGTTTTAAAAAAGCCGAAAA+TGG + chr5.1:27955365-27955384 MS.gene49655:CDS 30.0%
ACAAACAAAAACGACCCTTT+GGG + chr5.1:27955198-27955217 MS.gene49655:CDS 35.0%
AGTTTAGGGAGAAGTTGAAA+GGG + chr5.1:27955435-27955454 MS.gene49655:CDS 35.0%
GCAAAACATGCTTACATTTC+TGG + chr5.1:27955293-27955312 MS.gene49655:CDS 35.0%
TGAGTTCAGTTTCAGTTTCT+GGG + chr5.1:27955408-27955427 MS.gene49655:CDS 35.0%
TTATGGGAAGAAGTTGAAAG+TGG + chr5.1:27955323-27955342 MS.gene49655:CDS 35.0%
TTGAGTTCAGTTTCAGTTTC+TGG + chr5.1:27955407-27955426 MS.gene49655:CDS 35.0%
! AGTTTCTGGGTTTGAGTTTA+GGG + chr5.1:27955421-27955440 MS.gene49655:CDS 35.0%
! ATCCGAATCAGAACCATTTT+CGG - chr5.1:27955381-27955400 None:intergenic 35.0%
! TTTTTCACACCACCAATCTT+TGG - chr5.1:27955545-27955564 None:intergenic 35.0%
!! AAGATTGGTGGTGTGAAAAA+TGG + chr5.1:27955545-27955564 MS.gene49655:CDS 35.0%
!! ACTTGTTATAGGATGCAATG+GGG + chr5.1:27955710-27955729 MS.gene49655:CDS 35.0%
!!! GGGTTATGGTTTTGAATTGT+GGG + chr5.1:27955456-27955475 MS.gene49655:CDS 35.0%
!!! TGGTTTTGTTCTTCCAAAAG+TGG - chr5.1:27955179-27955198 None:intergenic 35.0%
ACAGTTTATGTTGGAGTTGC+AGG + chr5.1:27955589-27955608 MS.gene49655:CDS 40.0%
CACAAACAAAAACGACCCTT+TGG + chr5.1:27955197-27955216 MS.gene49655:CDS 40.0%
CAGTTTATGTTGGAGTTGCA+GGG + chr5.1:27955590-27955609 MS.gene49655:CDS 40.0%
GAATCAGTGTGAGTCTGTTT+GGG - chr5.1:27955031-27955050 None:intergenic 40.0%
GAGTTTAGGGAGAAGTTGAA+AGG + chr5.1:27955434-27955453 MS.gene49655:CDS 40.0%
GTTTAGGGAGAAGTTGAAAG+GGG + chr5.1:27955436-27955455 MS.gene49655:CDS 40.0%
TGAATCAGTGTGAGTCTGTT+TGG - chr5.1:27955032-27955051 None:intergenic 40.0%
! ATTCCCTTAGCTTTCAAAGC+AGG + chr5.1:27955101-27955120 MS.gene49655:CDS 40.0%
! CAGTTTCTGGGTTTGAGTTT+AGG + chr5.1:27955420-27955439 MS.gene49655:CDS 40.0%
! CTTGTTATAGGATGCAATGG+GGG + chr5.1:27955711-27955730 MS.gene49655:CDS 40.0%
! TGAATTGTGGGTTGACACTT+TGG + chr5.1:27955468-27955487 MS.gene49655:CDS 40.0%
! TTAGCTTTCAAAGCAGGGTT+AGG + chr5.1:27955107-27955126 MS.gene49655:CDS 40.0%
! TTCCCTTAGCTTTCAAAGCA+GGG + chr5.1:27955102-27955121 MS.gene49655:CDS 40.0%
!! AACCCTGCTTTGAAAGCTAA+GGG - chr5.1:27955107-27955126 None:intergenic 40.0%
!! AGCCGAAAATGGTTCTGATT+CGG + chr5.1:27955376-27955395 MS.gene49655:CDS 40.0%
!! TAACCCTGCTTTGAAAGCTA+AGG - chr5.1:27955108-27955127 None:intergenic 40.0%
!!! CAAAGGGTCGTTTTTGTTTG+TGG - chr5.1:27955199-27955218 None:intergenic 40.0%
!!! GGGGTTATGGTTTTGAATTG+TGG + chr5.1:27955455-27955474 MS.gene49655:CDS 40.0%
AGGTGTATCAGAGCTTGACA+AGG + chr5.1:27955268-27955287 MS.gene49655:CDS 45.0%
ATCCTAGGCTTAAAGGGGAT+TGG + chr5.1:27955660-27955679 MS.gene49655:CDS 45.0%
GAGAGTGATCCAAAGATTGG+TGG + chr5.1:27955533-27955552 MS.gene49655:CDS 45.0%
GGAGAAGTTGAAAGGGGTTA+TGG + chr5.1:27955442-27955461 MS.gene49655:CDS 45.0%
GGTGAGAGTGATCCAAAGAT+TGG + chr5.1:27955530-27955549 MS.gene49655:CDS 45.0%
TGGTCTCATGTCACTGTTGT+GGG + chr5.1:27955488-27955507 MS.gene49655:CDS 45.0%
TTCCAAAAGTGGCATTGCGT+CGG - chr5.1:27955168-27955187 None:intergenic 45.0%
TTGGTCTCATGTCACTGTTG+TGG + chr5.1:27955487-27955506 MS.gene49655:CDS 45.0%
AGGCTTAAAGGGGATTGGAG+TGG + chr5.1:27955665-27955684 MS.gene49655:CDS 50.0%
CTCCAATCCCCTTTAAGCCT+AGG - chr5.1:27955665-27955684 None:intergenic 50.0%
CTCTCACCATGTGCATAACG+TGG - chr5.1:27955518-27955537 None:intergenic 50.0%
GGTCTCATGTCACTGTTGTG+GGG + chr5.1:27955489-27955508 MS.gene49655:CDS 50.0%
TATAGGATGCAATGGGGGAC+TGG + chr5.1:27955716-27955735 MS.gene49655:CDS 50.0%
CAAAAGTGGCATTGCGTCGG+TGG - chr5.1:27955165-27955184 None:intergenic 55.0%
GGGACACCACGTTATGCACA+TGG + chr5.1:27955509-27955528 MS.gene49655:CDS 55.0%
!! CTGGTCTTAGGTGTGAGGGA+TGG + chr5.1:27955735-27955754 MS.gene49655:CDS 55.0%
!! GGGACTGGTCTTAGGTGTGA+GGG + chr5.1:27955731-27955750 MS.gene49655:CDS 55.0%
!! TGCAATGGGGGACTGGTCTT+AGG + chr5.1:27955723-27955742 MS.gene49655:CDS 55.0%
!!! CACCGACGCAATGCCACTTT+TGG + chr5.1:27955163-27955182 MS.gene49655:CDS 55.0%
ATGGAAGTCTCGCGCAGACG+AGG + chr5.1:27955754-27955773 MS.gene49655:CDS 60.0%
TCTCGCGCAGACGAGGAAAC+TGG + chr5.1:27955761-27955780 MS.gene49655:CDS 60.0%
!! GGGGACTGGTCTTAGGTGTG+AGG + chr5.1:27955730-27955749 MS.gene49655:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 27954996 27955802 27954996 ID=MS.gene49655
chr5.1 mRNA 27954996 27955802 27954996 ID=MS.gene49655.t1;Parent=MS.gene49655
chr5.1 exon 27954996 27955802 27954996 ID=MS.gene49655.t1.exon1;Parent=MS.gene49655.t1
chr5.1 CDS 27954996 27955802 27954996 ID=cds.MS.gene49655.t1;Parent=MS.gene49655.t1
Gene Sequence

>MS.gene49655

ATGAAGAAGGTGAAGCTTGAAGTGTTCACAGTCCCAAACAGACTCACACTGATTCAAATTTTCATGATGATGATGCTTCTCTACATGCTTTTCATGAGCTTTGAAATTCCCTTAGCTTTCAAAGCAGGGTTAGGTTCAGAAACTGTAGCTAATATTGGGGTTTTCACCACCGACGCAATGCCACTTTTGGAAGAACAAAACCACAAACAAAAACGACCCTTTGGGCTATTATTACAGAAAGTTTCAACCTTGAGCTTTAACAATAGCTTTGAAGGTGTATCAGAGCTTGACAAGGTAGCAAAACATGCTTACATTTCTGGTAAAAAGTTATGGGAAGAAGTTGAAAGTGGTAAAGTTAAGAGTTTTTCGGGTTTTAAAAAAGCCGAAAATGGTTCTGATTCGGATACTTGTTTGAGTTCAGTTTCAGTTTCTGGGTTTGAGTTTAGGGAGAAGTTGAAAGGGGTTATGGTTTTGAATTGTGGGTTGACACTTTGGTCTCATGTCACTGTTGTGGGGACACCACGTTATGCACATGGTGAGAGTGATCCAAAGATTGGTGGTGTGAAAAATGGTGATGAAAAGGTTTTGGTTTCACAGTTTATGTTGGAGTTGCAGGGTTTGAAAGCTGTTGATAATGAAGAGCCACCAAAGATATTGCATTTTAATCCTAGGCTTAAAGGGGATTGGAGTGGAAAGCCTGTTATTGAACAGAATACTTGTTATAGGATGCAATGGGGGACTGGTCTTAGGTGTGAGGGATGGAAGTCTCGCGCAGACGAGGAAACTGGTAATGTATTAGCCCTTTGA

Protein sequence

>MS.gene49655.t1

MKKVKLEVFTVPNRLTLIQIFMMMMLLYMLFMSFEIPLAFKAGLGSETVANIGVFTTDAMPLLEEQNHKQKRPFGLLLQKVSTLSFNNSFEGVSELDKVAKHAYISGKKLWEEVESGKVKSFSGFKKAENGSDSDTCLSSVSVSGFEFREKLKGVMVLNCGLTLWSHVTVVGTPRYAHGESDPKIGGVKNGDEKVLVSQFMLELQGLKAVDNEEPPKILHFNPRLKGDWSGKPVIEQNTCYRMQWGTGLRCEGWKSRADEETGNVLAL