AlfalfaGEDB | MS.gene49750

M. sativa cultivar XinJiangDaYe / MS.gene49750

KEGG: -
GO: -
PFAM: PF05678.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49750.t1	XP_003630432.2	98.4	192	3	0	1	192	1	192	7.80E-99	369.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49750.t1	Q9FNP0	57.0	179	55	6	16	191	13	172	7.8e-39	161.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49750.t1	G7LGI0	98.4	192	3	0	1	192	1	192	5.6e-99	369.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049222	MS.gene49750	0.831406	1.70E-55	-1.69E-46
MS.gene049232	MS.gene49750	0.801089	1.06E-48	-1.69E-46
MS.gene049609	MS.gene49750	0.828677	7.87E-55	-1.69E-46
MS.gene049805	MS.gene49750	0.801751	7.77E-49	-1.69E-46
MS.gene050323	MS.gene49750	0.805319	1.41E-49	-1.69E-46
MS.gene050593	MS.gene49750	0.810189	1.30E-50	-1.69E-46
MS.gene050747	MS.gene49750	0.819139	1.36E-52	-1.69E-46
MS.gene050772	MS.gene49750	0.834467	2.95E-56	-1.69E-46
MS.gene050950	MS.gene49750	0.813934	1.99E-51	-1.69E-46
MS.gene052916	MS.gene49750	0.823434	1.39E-53	-1.69E-46
MS.gene053574	MS.gene49750	0.833086	6.53E-56	-1.69E-46
MS.gene053824	MS.gene49750	0.814015	1.91E-51	-1.69E-46
MS.gene054003	MS.gene49750	0.805637	1.21E-49	-1.69E-46
MS.gene055577	MS.gene49750	0.832292	1.03E-55	-1.69E-46
MS.gene055722	MS.gene49750	0.812912	3.34E-51	-1.69E-46
MS.gene055941	MS.gene49750	0.810558	1.09E-50	-1.69E-46
MS.gene056448	MS.gene49750	0.811936	5.46E-51	-1.69E-46
MS.gene057076	MS.gene49750	0.804198	2.42E-49	-1.69E-46
MS.gene05837	MS.gene49750	0.809101	2.23E-50	-1.69E-46
MS.gene059801	MS.gene49750	0.817787	2.75E-52	-1.69E-46
MS.gene060482	MS.gene49750	0.802361	5.82E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49750.t1	MTR_8g095470	98.438	192	3	0	1	192	1	192	5.91e-138	383
MS.gene49750.t1	MTR_4g088695	34.054	185	94	6	20	188	61	233	8.13e-13	65.1
MS.gene49750.t1	MTR_2g013950	34.826	201	93	10	7	188	71	252	6.28e-12	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49750.t1	AT5G08480	56.983	179	55	6	16	191	13	172	6.51e-46	149
MS.gene49750.t1	AT5G08480	56.983	179	55	6	16	191	13	172	6.51e-46	149
MS.gene49750.t1	AT5G08480	47.486	179	41	5	16	191	13	141	7.22e-31	110
MS.gene49750.t1	AT5G53830	32.828	198	95	5	20	186	49	239	4.24e-12	63.2
MS.gene49750.t1	AT1G28280	33.810	210	87	11	11	188	49	238	8.67e-11	59.7
MS.gene49750.t1	AT1G28280	33.810	210	87	11	11	188	49	238	8.71e-11	59.7

Find 49 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACACAAACACAGGTGCATTT+AGG	0.187922	4.4:+7454671	MS.gene49750:CDS
GTTCTCTCAGTTAACTATTA+TGG	0.217006	4.4:+7454975	MS.gene49750:CDS
TTCCTTCTTTCATGGAGTTT+TGG	0.237618	4.4:-7454787	None:intergenic
GGAAACAAGTTAAGCAATTC+TGG	0.246716	4.4:-7455123	None:intergenic
TCATGCATTTCCTTCTTTCA+TGG	0.261349	4.4:-7454795	None:intergenic
GGTGGTGTTCTCTTCGTAAT+AGG	0.264699	4.4:-7454766	None:intergenic
GGTTTAACAATCTCAAGTTT+TGG	0.319524	4.4:-7454820	None:intergenic
CACAAACACAGGTGCATTTA+GGG	0.321361	4.4:+7454672	MS.gene49750:CDS
TTTCCTTCAAGTCCTGTATC+AGG	0.338299	4.4:+7454904	MS.gene49750:CDS
GAAGTAGTGCAAAGATTAAC+AGG	0.361566	4.4:+7454694	MS.gene49750:CDS
TGTTTGTGATATTGAAGATT+TGG	0.376002	4.4:-7454841	None:intergenic
TAGGTGCAACCTTTGTAGTT+TGG	0.379631	4.4:-7454747	None:intergenic
GTGTGAACAAATGTTGTTAG+TGG	0.401194	4.4:-7454652	None:intergenic
CTTTGTTGCATTGCCTTCTG+AGG	0.427730	4.4:-7454719	None:intergenic
CCAGTACATGGTTCAACAAC+AGG	0.437636	4.4:+7454619	MS.gene49750:CDS
CATACTTTCTCACATGCCTT+AGG	0.439034	4.4:-7455156	None:intergenic
TTTGTTGCATTGCCTTCTGA+GGG	0.457769	4.4:-7454718	None:intergenic
GTTTCCACTGGCATCTCCTA+AGG	0.476906	4.4:+7455140	MS.gene49750:CDS
GAATTTCTTGACTTAGAAGA+AGG	0.481290	4.4:-7454877	None:intergenic
CTTGCTTTAGACCTGGGTGA+TGG	0.493410	4.4:-7455087	None:intergenic
GAACTTCCACTACCTGATAC+AGG	0.493636	4.4:-7454916	None:intergenic
GTGGAAGGTGTGGTAGGGCT+TGG	0.499380	4.4:-7454949	None:intergenic
CTACCTGATACAGGACTTGA+AGG	0.504395	4.4:-7454907	None:intergenic
ATTGCTTAACTTGTTTCCAC+TGG	0.509456	4.4:+7455128	MS.gene49750:CDS
GAAGCCACTTGCTTTAGACC+TGG	0.517471	4.4:-7455094	None:intergenic
TCAAGTCCTGTATCAGGTAG+TGG	0.519505	4.4:+7454910	MS.gene49750:CDS
AAGCCACTTGCTTTAGACCT+GGG	0.539510	4.4:-7455093	None:intergenic
CACCAAAACTCCATGAAAGA+AGG	0.543366	4.4:+7454785	MS.gene49750:CDS
CTCAGAAGGCAATGCAACAA+AGG	0.546327	4.4:+7454720	MS.gene49750:CDS
ATGGAGAAGCCACCAGTACA+TGG	0.555074	4.4:+7454607	MS.gene49750:CDS
CCTGTTGTTGAACCATGTAC+TGG	0.565708	4.4:-7454619	None:intergenic
AACAATGTGGAAGGTGTGGT+AGG	0.566267	4.4:-7454955	None:intergenic
AAATCTGATTGTGATGGGAA+TGG	0.567485	4.4:+7454588	None:intergenic
AGATTAACAGGTCCCTCAGA+AGG	0.568541	4.4:+7454706	MS.gene49750:CDS
ACAATGTGGAAGGTGTGGTA+GGG	0.605585	4.4:-7454954	None:intergenic
GTTGTTGAACCATGTACTGG+TGG	0.616693	4.4:-7454616	None:intergenic
TGGAAGATGAAAAGAAACAA+GGG	0.617549	4.4:+7454995	MS.gene49750:CDS
ACATTTGTTCACACAAACAC+AGG	0.619702	4.4:+7454661	MS.gene49750:CDS
ATGGAAGATGAAAAGAAACA+AGG	0.623994	4.4:+7454994	MS.gene49750:CDS
AACTGAGAGAACAATGTGGA+AGG	0.625030	4.4:-7454964	None:intergenic
CTTCAATATCACAAACAACC+AGG	0.631097	4.4:+7454847	MS.gene49750:CDS
TCACCCAGGTCTAAAGCAAG+TGG	0.631290	4.4:+7455090	MS.gene49750:CDS
AGAAAGCTATCAAAGAGAGA+CGG	0.633848	4.4:+7455052	MS.gene49750:CDS
TTTATTTGCATCCATCACCC+AGG	0.643004	4.4:+7455076	MS.gene49750:CDS
TTAGAAGAAGGTGATGCACC+TGG	0.665275	4.4:-7454865	None:intergenic
AAAGGAAGACCAAACTACAA+AGG	0.666842	4.4:+7454738	MS.gene49750:CDS
AGAGAACAATGTGGAAGGTG+TGG	0.689227	4.4:-7454959	None:intergenic
CATGCCTTAGGAGATGCCAG+TGG	0.723253	4.4:-7455144	None:intergenic
AGTTAACTGAGAGAACAATG+TGG	0.758270	4.4:-7454968	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TGTTTGTGATATTGAAGATT+TGG	-	chr4.4:7454844-7454863	None:intergenic	25.0%
	ATGGAAGATGAAAAGAAACA+AGG	+	chr4.4:7454994-7455013	MS.gene49750:CDS	30.0%
	GAATTTCTTGACTTAGAAGA+AGG	-	chr4.4:7454880-7454899	None:intergenic	30.0%
	GTTCTCTCAGTTAACTATTA+TGG	+	chr4.4:7454975-7454994	MS.gene49750:CDS	30.0%
	TGGAAGATGAAAAGAAACAA+GGG	+	chr4.4:7454995-7455014	MS.gene49750:CDS	30.0%
!	GGTTTAACAATCTCAAGTTT+TGG	-	chr4.4:7454823-7454842	None:intergenic	30.0%
	AAAGGAAGACCAAACTACAA+AGG	+	chr4.4:7454738-7454757	MS.gene49750:CDS	35.0%
	ACATTTGTTCACACAAACAC+AGG	+	chr4.4:7454661-7454680	MS.gene49750:CDS	35.0%
	AGAAAGCTATCAAAGAGAGA+CGG	+	chr4.4:7455052-7455071	MS.gene49750:CDS	35.0%
	AGTTAACTGAGAGAACAATG+TGG	-	chr4.4:7454971-7454990	None:intergenic	35.0%
	CTTCAATATCACAAACAACC+AGG	+	chr4.4:7454847-7454866	MS.gene49750:CDS	35.0%
	GAAGTAGTGCAAAGATTAAC+AGG	+	chr4.4:7454694-7454713	MS.gene49750:CDS	35.0%
	GGAAACAAGTTAAGCAATTC+TGG	-	chr4.4:7455126-7455145	None:intergenic	35.0%
	GTGTGAACAAATGTTGTTAG+TGG	-	chr4.4:7454655-7454674	None:intergenic	35.0%
	TCATGCATTTCCTTCTTTCA+TGG	-	chr4.4:7454798-7454817	None:intergenic	35.0%
!	TTCCTTCTTTCATGGAGTTT+TGG	-	chr4.4:7454790-7454809	None:intergenic	35.0%
!!	ATTGCTTAACTTGTTTCCAC+TGG	+	chr4.4:7455128-7455147	MS.gene49750:CDS	35.0%
	AACTGAGAGAACAATGTGGA+AGG	-	chr4.4:7454967-7454986	None:intergenic	40.0%
	ACACAAACACAGGTGCATTT+AGG	+	chr4.4:7454671-7454690	MS.gene49750:CDS	40.0%
	CACAAACACAGGTGCATTTA+GGG	+	chr4.4:7454672-7454691	MS.gene49750:CDS	40.0%
	CACCAAAACTCCATGAAAGA+AGG	+	chr4.4:7454785-7454804	MS.gene49750:CDS	40.0%
	CATACTTTCTCACATGCCTT+AGG	-	chr4.4:7455159-7455178	None:intergenic	40.0%
	TAGGTGCAACCTTTGTAGTT+TGG	-	chr4.4:7454750-7454769	None:intergenic	40.0%
	TTTATTTGCATCCATCACCC+AGG	+	chr4.4:7455076-7455095	MS.gene49750:CDS	40.0%
	TTTCCTTCAAGTCCTGTATC+AGG	+	chr4.4:7454904-7454923	MS.gene49750:CDS	40.0%
	TTTGTTGCATTGCCTTCTGA+GGG	-	chr4.4:7454721-7454740	None:intergenic	40.0%
!!	CTTCTTTCATGGAGTTTTGG+TGG	-	chr4.4:7454787-7454806	None:intergenic	40.0%
	AGAGAACAATGTGGAAGGTG+TGG	-	chr4.4:7454962-7454981	None:intergenic	45.0%
	AGATTAACAGGTCCCTCAGA+AGG	+	chr4.4:7454706-7454725	MS.gene49750:CDS	45.0%
	CCAGTACATGGTTCAACAAC+AGG	+	chr4.4:7454619-7454638	MS.gene49750:CDS	45.0%
	CCTGTTGTTGAACCATGTAC+TGG	-	chr4.4:7454622-7454641	None:intergenic	45.0%
	CTACCTGATACAGGACTTGA+AGG	-	chr4.4:7454910-7454929	None:intergenic	45.0%
	CTCAGAAGGCAATGCAACAA+AGG	+	chr4.4:7454720-7454739	MS.gene49750:CDS	45.0%
	CTTTGTTGCATTGCCTTCTG+AGG	-	chr4.4:7454722-7454741	None:intergenic	45.0%
	GAACTTCCACTACCTGATAC+AGG	-	chr4.4:7454919-7454938	None:intergenic	45.0%
	GGTGGTGTTCTCTTCGTAAT+AGG	-	chr4.4:7454769-7454788	None:intergenic	45.0%
	GTTGTTGAACCATGTACTGG+TGG	-	chr4.4:7454619-7454638	None:intergenic	45.0%
	TCAAGTCCTGTATCAGGTAG+TGG	+	chr4.4:7454910-7454929	MS.gene49750:CDS	45.0%
	TTAGAAGAAGGTGATGCACC+TGG	-	chr4.4:7454868-7454887	None:intergenic	45.0%
!	AACAATGTGGAAGGTGTGGT+AGG	-	chr4.4:7454958-7454977	None:intergenic	45.0%
!	AAGCCACTTGCTTTAGACCT+GGG	-	chr4.4:7455096-7455115	None:intergenic	45.0%
!	ACAATGTGGAAGGTGTGGTA+GGG	-	chr4.4:7454957-7454976	None:intergenic	45.0%
	ATGGAGAAGCCACCAGTACA+TGG	+	chr4.4:7454607-7454626	MS.gene49750:CDS	50.0%
	GTTTCCACTGGCATCTCCTA+AGG	+	chr4.4:7455140-7455159	MS.gene49750:CDS	50.0%
!	CTTGCTTTAGACCTGGGTGA+TGG	-	chr4.4:7455090-7455109	None:intergenic	50.0%
!	GAAGCCACTTGCTTTAGACC+TGG	-	chr4.4:7455097-7455116	None:intergenic	50.0%
!	TCACCCAGGTCTAAAGCAAG+TGG	+	chr4.4:7455090-7455109	MS.gene49750:CDS	50.0%
	CATGCCTTAGGAGATGCCAG+TGG	-	chr4.4:7455147-7455166	None:intergenic	55.0%
!	GTGGAAGGTGTGGTAGGGCT+TGG	-	chr4.4:7454952-7454971	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	7454601	7455179	7454601	ID=MS.gene49750
chr4.4	mRNA	7454601	7455179	7454601	ID=MS.gene49750.t1;Parent=MS.gene49750
chr4.4	exon	7454601	7455179	7454601	ID=MS.gene49750.t1.exon1;Parent=MS.gene49750.t1
chr4.4	CDS	7454601	7455179	7454601	ID=cds.MS.gene49750.t1;Parent=MS.gene49750.t1

Gene Sequence

>MS.gene49750

ATGGGAATGGAGAAGCCACCAGTACATGGTTCAACAACAGGTGATTGTAAACCACTAACAACATTTGTTCACACAAACACAGGTGCATTTAGGGAAGTAGTGCAAAGATTAACAGGTCCCTCAGAAGGCAATGCAACAAAGGAAGACCAAACTACAAAGGTTGCACCTATTACGAAGAGAACACCACCAAAACTCCATGAAAGAAGGAAATGCATGAAGCCAAAACTTGAGATTGTTAAACCAAATCTTCAATATCACAAACAACCAGGTGCATCACCTTCTTCTAAGTCAAGAAATTCTAGCTTTCCTTCAAGTCCTGTATCAGGTAGTGGAAGTTCTAGTCTTCTTCCAAGCCCTACCACACCTTCCACATTGTTCTCTCAGTTAACTATTATGGAAGATGAAAAGAAACAAGGGTCTGTGATAAATGAAATAAACATAGAAGAAGAAGAGAAAGCTATCAAAGAGAGACGGTTTTATTTGCATCCATCACCCAGGTCTAAAGCAAGTGGCTTCAATGAACCAGAATTGCTTAACTTGTTTCCACTGGCATCTCCTAAGGCATGTGAGAAAGTATGA

Protein sequence

>MS.gene49750.t1

MGMEKPPVHGSTTGDCKPLTTFVHTNTGAFREVVQRLTGPSEGNATKEDQTTKVAPITKRTPPKLHERRKCMKPKLEIVKPNLQYHKQPGASPSSKSRNSSFPSSPVSGSGSSSLLPSPTTPSTLFSQLTIMEDEKKQGSVINEINIEEEEKAIKERRFYLHPSPRSKASGFNEPELLNLFPLASPKACEKV