Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50503.t1 | KEH23791.1 | 82.7 | 98 | 10 | 2 | 1 | 91 | 1 | 98 | 1.10E-32 | 149.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50503.t1 | A0A072U353 | 82.7 | 98 | 10 | 2 | 1 | 91 | 1 | 98 | 8.2e-33 | 149.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050809 | MS.gene50503 | 0.806519 | 7.91E-50 | -1.69E-46 |
| MS.gene05104 | MS.gene50503 | 0.801686 | 8.01E-49 | -1.69E-46 |
| MS.gene051138 | MS.gene50503 | 0.803161 | 3.98E-49 | -1.69E-46 |
| MS.gene051651 | MS.gene50503 | 0.800153 | 1.65E-48 | -1.69E-46 |
| MS.gene05240 | MS.gene50503 | 0.825826 | 3.79E-54 | -1.69E-46 |
| MS.gene053920 | MS.gene50503 | 0.813138 | 2.98E-51 | -1.69E-46 |
| MS.gene056590 | MS.gene50503 | 0.808862 | 2.51E-50 | -1.69E-46 |
| MS.gene056591 | MS.gene50503 | 0.805541 | 1.27E-49 | -1.69E-46 |
| MS.gene056697 | MS.gene50503 | 0.80482 | 1.80E-49 | -1.69E-46 |
| MS.gene056856 | MS.gene50503 | 0.832094 | 1.15E-55 | -1.69E-46 |
| MS.gene057717 | MS.gene50503 | 0.825233 | 5.24E-54 | -1.69E-46 |
| MS.gene058067 | MS.gene50503 | 0.817723 | 2.84E-52 | -1.69E-46 |
| MS.gene058068 | MS.gene50503 | 0.809718 | 1.65E-50 | -1.69E-46 |
| MS.gene058099 | MS.gene50503 | 0.818913 | 1.53E-52 | -1.69E-46 |
| MS.gene059069 | MS.gene50503 | 0.803881 | 2.82E-49 | -1.69E-46 |
| MS.gene059315 | MS.gene50503 | 0.800654 | 1.30E-48 | -1.69E-46 |
| MS.gene060405 | MS.gene50503 | 0.801952 | 7.06E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50503.t1 | MTR_7g095250 | 84.043 | 94 | 9 | 1 | 1 | 88 | 1 | 94 | 6.88e-49 | 153 |
| MS.gene50503.t1 | MTR_1g071720 | 62.500 | 96 | 30 | 6 | 1 | 93 | 36 | 128 | 1.91e-28 | 102 |
| MS.gene50503.t1 | MTR_1g071720 | 62.500 | 96 | 30 | 6 | 1 | 93 | 36 | 128 | 1.97e-28 | 101 |
| MS.gene50503.t1 | MTR_1g103420 | 48.387 | 93 | 47 | 1 | 2 | 94 | 37 | 128 | 2.20e-23 | 89.4 |
| MS.gene50503.t1 | MTR_5g006940 | 47.126 | 87 | 45 | 1 | 1 | 87 | 36 | 121 | 3.74e-21 | 83.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50503.t1 | AT2G48140 | 46.429 | 84 | 45 | 0 | 4 | 87 | 33 | 116 | 1.27e-21 | 82.8 |
| MS.gene50503.t1 | AT2G48140 | 46.429 | 84 | 45 | 0 | 4 | 87 | 33 | 116 | 2.80e-21 | 83.6 |
| MS.gene50503.t1 | AT1G05450 | 46.067 | 89 | 43 | 3 | 4 | 92 | 39 | 122 | 1.76e-17 | 73.9 |
| MS.gene50503.t1 | AT3G22620 | 45.455 | 77 | 41 | 1 | 4 | 80 | 35 | 110 | 7.70e-11 | 56.6 |
Find 22 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCTTTGATGGGCACTAATA+TGG | 0.225608 | 7.3:-15877994 | MS.gene50503:CDS |
| CTACTTGTTGTGATTCATTA+AGG | 0.267679 | 7.3:-15878016 | MS.gene50503:CDS |
| ATTGCAAGAACTTGGTTAAT+AGG | 0.287987 | 7.3:+15877924 | None:intergenic |
| TGATTCATTAAGGTCTTTGA+TGG | 0.371159 | 7.3:-15878006 | MS.gene50503:CDS |
| CCTTGTGCTAACATCATAAC+AGG | 0.384512 | 7.3:-15878068 | MS.gene50503:CDS |
| ATAACAGGAAGCACTAATAA+TGG | 0.421549 | 7.3:-15878053 | MS.gene50503:CDS |
| GAACTTGGTTAATAGGTAGT+TGG | 0.432984 | 7.3:+15877931 | None:intergenic |
| TATTAGTAGTGGTGTTTGTT+GGG | 0.439335 | 7.3:-15877623 | MS.gene50503:intron |
| GAGCATTGGAAGACAACATA+AGG | 0.445627 | 7.3:+15877961 | None:intergenic |
| ATACATGCCTAGAGCTGGTA+AGG | 0.456573 | 7.3:+15877728 | None:intergenic |
| GAATCACAACAAGTAGTTGA+TGG | 0.473600 | 7.3:+15878023 | None:intergenic |
| AAAGAGACATTGCAAGAACT+TGG | 0.476462 | 7.3:+15877916 | None:intergenic |
| CTATTAGTAGTGGTGTTTGT+TGG | 0.481034 | 7.3:-15877624 | MS.gene50503:intron |
| GATTCATTAAGGTCTTTGAT+GGG | 0.535229 | 7.3:-15878005 | MS.gene50503:CDS |
| TAGTTGGAAAATTTGAGCAT+TGG | 0.538312 | 7.3:+15877947 | None:intergenic |
| AGAGCTGGTAAGGGAGAACT+TGG | 0.546288 | 7.3:+15877738 | None:intergenic |
| AATCAATACATGCCTAGAGC+TGG | 0.556293 | 7.3:+15877723 | None:intergenic |
| TCTCAAGCATGTAATATCAA+AGG | 0.562021 | 7.3:-15877894 | MS.gene50503:CDS |
| AGTTCTCCCTTACCAGCTCT+AGG | 0.581069 | 7.3:-15877735 | MS.gene50503:CDS |
| GGAGCTTCTCTTCAGTGCAA+AGG | 0.609225 | 7.3:-15877873 | MS.gene50503:intron |
| CCTGTTATGATGTTAGCACA+AGG | 0.620375 | 7.3:+15878068 | None:intergenic |
| TACATGCCTAGAGCTGGTAA+GGG | 0.659627 | 7.3:+15877729 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGACAGTAAAATAAAGTTTT+GGG | + | chr7.3:15878036-15878055 | None:intergenic | 20.0% |
| !!! | TTGACAGTAAAATAAAGTTT+TGG | + | chr7.3:15878037-15878056 | None:intergenic | 20.0% |
| ACAACTATGACTATTAGTAG+TGG | - | chr7.3:15878070-15878089 | MS.gene50503:CDS | 30.0% | |
| ATTGCAAGAACTTGGTTAAT+AGG | + | chr7.3:15877783-15877802 | None:intergenic | 30.0% | |
| GATTCATTAAGGTCTTTGAT+GGG | - | chr7.3:15877699-15877718 | MS.gene50503:intron | 30.0% | |
| TAGTTGGAAAATTTGAGCAT+TGG | + | chr7.3:15877760-15877779 | None:intergenic | 30.0% | |
| TATTAGTAGTGGTGTTTGTT+GGG | - | chr7.3:15878081-15878100 | MS.gene50503:CDS | 30.0% | |
| TCTCAAGCATGTAATATCAA+AGG | - | chr7.3:15877810-15877829 | MS.gene50503:intron | 30.0% | |
| TGATTCATTAAGGTCTTTGA+TGG | - | chr7.3:15877698-15877717 | MS.gene50503:intron | 30.0% | |
| !! | ATAACAGGAAGCACTAATAA+TGG | - | chr7.3:15877651-15877670 | MS.gene50503:intron | 30.0% |
| !! | CTACTTGTTGTGATTCATTA+AGG | - | chr7.3:15877688-15877707 | MS.gene50503:intron | 30.0% |
| AAAGAGACATTGCAAGAACT+TGG | + | chr7.3:15877791-15877810 | None:intergenic | 35.0% | |
| CTATTAGTAGTGGTGTTTGT+TGG | - | chr7.3:15878080-15878099 | MS.gene50503:CDS | 35.0% | |
| GAACTTGGTTAATAGGTAGT+TGG | + | chr7.3:15877776-15877795 | None:intergenic | 35.0% | |
| GAATCACAACAAGTAGTTGA+TGG | + | chr7.3:15877684-15877703 | None:intergenic | 35.0% | |
| AATCAATACATGCCTAGAGC+TGG | + | chr7.3:15877984-15878003 | None:intergenic | 40.0% | |
| CCTGTTATGATGTTAGCACA+AGG | + | chr7.3:15877639-15877658 | None:intergenic | 40.0% | |
| CCTTGTGCTAACATCATAAC+AGG | - | chr7.3:15877636-15877655 | MS.gene50503:intron | 40.0% | |
| ! | GTCTTTGATGGGCACTAATA+TGG | - | chr7.3:15877710-15877729 | MS.gene50503:intron | 40.0% |
| !! | GAGCATTGGAAGACAACATA+AGG | + | chr7.3:15877746-15877765 | None:intergenic | 40.0% |
| ATACATGCCTAGAGCTGGTA+AGG | + | chr7.3:15877979-15877998 | None:intergenic | 45.0% | |
| TACATGCCTAGAGCTGGTAA+GGG | + | chr7.3:15877978-15877997 | None:intergenic | 45.0% | |
| AGAGCTGGTAAGGGAGAACT+TGG | + | chr7.3:15877969-15877988 | None:intergenic | 50.0% | |
| AGTTCTCCCTTACCAGCTCT+AGG | - | chr7.3:15877969-15877988 | MS.gene50503:CDS | 50.0% | |
| GGAGCTTCTCTTCAGTGCAA+AGG | - | chr7.3:15877831-15877850 | MS.gene50503:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 15877615 | 15878111 | 15877615 | ID=MS.gene50503 |
| chr7.3 | mRNA | 15877615 | 15878111 | 15877615 | ID=MS.gene50503.t1;Parent=MS.gene50503 |
| chr7.3 | exon | 15877874 | 15878111 | 15877874 | ID=MS.gene50503.t1.exon1;Parent=MS.gene50503.t1 |
| chr7.3 | CDS | 15877874 | 15878111 | 15877874 | ID=cds.MS.gene50503.t1;Parent=MS.gene50503.t1 |
| chr7.3 | exon | 15877732 | 15877777 | 15877732 | ID=MS.gene50503.t1.exon2;Parent=MS.gene50503.t1 |
| chr7.3 | CDS | 15877732 | 15877777 | 15877732 | ID=cds.MS.gene50503.t1;Parent=MS.gene50503.t1 |
| chr7.3 | exon | 15877615 | 15877636 | 15877615 | ID=MS.gene50503.t1.exon3;Parent=MS.gene50503.t1 |
| chr7.3 | CDS | 15877615 | 15877636 | 15877615 | ID=cds.MS.gene50503.t1;Parent=MS.gene50503.t1 |
| Gene Sequence |
| Protein sequence |
