AlfalfaGEDB | MS.gene053920

M. sativa cultivar XinJiangDaYe / MS.gene053920

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene053920.t1	XP_013442744.1	96.7	121	4	0	1	121	81	201	9.40E-58	232.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene053920.t1	C6TG62	75.7	115	28	0	1	115	80	194	5.1e-44	178.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene053920.t1	A0A072TGS8	96.7	121	4	0	1	121	81	201	6.8e-58	232.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene053920	MS.gene057717	0.839404	1.62E-57	-1.69E-46
MS.gene053920	MS.gene058067	0.808807	2.58E-50	-1.69E-46
MS.gene053920	MS.gene05926	0.804533	2.06E-49	-1.69E-46
MS.gene053920	MS.gene068037	0.80531	1.42E-49	-1.69E-46
MS.gene053920	MS.gene070268	0.800267	1.56E-48	-1.69E-46
MS.gene053920	MS.gene07082	-0.816006	6.91E-52	-1.69E-46
MS.gene053920	MS.gene071405	0.80307	4.15E-49	-1.69E-46
MS.gene053920	MS.gene072074	0.836152	1.11E-56	-1.69E-46
MS.gene053920	MS.gene073297	0.802702	4.95E-49	-1.69E-46
MS.gene053920	MS.gene074291	-0.800195	1.61E-48	-1.69E-46
MS.gene053920	MS.gene21795	0.832828	7.56E-56	-1.69E-46
MS.gene053920	MS.gene22400	0.805067	1.60E-49	-1.69E-46
MS.gene053920	MS.gene33596	0.803938	2.75E-49	-1.69E-46
MS.gene053920	MS.gene35795	0.809494	1.84E-50	-1.69E-46
MS.gene053920	MS.gene35853	0.818637	1.77E-52	-1.69E-46
MS.gene053920	MS.gene35931	0.811104	8.27E-51	-1.69E-46
MS.gene053920	MS.gene36961	0.80923	2.10E-50	-1.69E-46
MS.gene053920	MS.gene40492	0.806646	7.44E-50	-1.69E-46
MS.gene053920	MS.gene44877	0.825464	4.62E-54	-1.69E-46
MS.gene053920	MS.gene45697	0.806949	6.42E-50	-1.69E-46
MS.gene053920	MS.gene47926	0.825927	3.59E-54	-1.69E-46
MS.gene053920	MS.gene48786	0.800342	1.51E-48	-1.69E-46
MS.gene053920	MS.gene50248	0.816002	6.92E-52	-1.69E-46
MS.gene053920	MS.gene50503	0.813138	2.98E-51	-1.69E-46
MS.gene053920	MS.gene52084	0.828209	1.02E-54	-1.69E-46
MS.gene053920	MS.gene53494	0.82656	2.54E-54	-1.69E-46
MS.gene053920	MS.gene54347	0.801546	8.56E-49	-1.69E-46
MS.gene053920	MS.gene59400	0.807882	4.07E-50	-1.69E-46
MS.gene053920	MS.gene60307	0.805546	1.27E-49	-1.69E-46
MS.gene053920	MS.gene60853	0.822232	2.65E-53	-1.69E-46
MS.gene053920	MS.gene62884	0.80233	5.91E-49	-1.69E-46
MS.gene053920	MS.gene63011	0.800587	1.34E-48	-1.69E-46
MS.gene053920	MS.gene63012	0.821352	4.23E-53	-1.69E-46
MS.gene053920	MS.gene69873	0.908929	9.98E-82	-1.69E-46
MS.gene053920	MS.gene71058	-0.804051	2.60E-49	-1.69E-46
MS.gene053920	MS.gene75261	0.821191	4.60E-53	-1.69E-46
MS.gene053920	MS.gene78728	0.973731	6.28E-137	-1.69E-46
MS.gene053920	MS.gene79518	0.805579	1.25E-49	-1.69E-46
MS.gene053920	MS.gene80144	0.950771	8.26E-109	-1.69E-46
MS.gene053920	MS.gene88769	0.817289	3.56E-52	-1.69E-46
MS.gene053920	MS.gene91317	0.832536	8.94E-56	-1.69E-46
MS.gene053920	MS.gene93196	0.821567	3.77E-53	-1.69E-46
MS.gene053920	MS.gene93435	0.918813	9.80E-87	-1.69E-46
MS.gene053920	MS.gene93739	0.802189	6.31E-49	-1.69E-46
MS.gene053920	MS.gene95478	0.808035	3.78E-50	-1.69E-46
MS.gene053920	MS.gene96335	0.805702	1.17E-49	-1.69E-46
MS.gene053920	MS.gene96549	-0.800852	1.19E-48	-1.69E-46
MS.gene053920	MS.gene96701	0.802073	6.67E-49	-1.69E-46
MS.gene053920	MS.gene98021	0.839029	2.02E-57	-1.69E-46
MS.gene053920	MS.gene98078	0.8144	1.57E-51	-1.69E-46
MS.gene053920	MS.gene98218	-0.807093	5.98E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 32 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCCCTTATTGCTTCTTTCAT+TGG	0.228754	5.3:-42812172	MS.gene053920:CDS
AAGTCCTTTGTAATCATATT+GGG	0.274380	5.3:+42812472	None:intergenic
GAAGTCCTTTGTAATCATAT+TGG	0.328703	5.3:+42812471	None:intergenic
AGCCAATGAAAGAAGCAATA+AGG	0.340360	5.3:+42812170	None:intergenic
TTTGAAAGCTATTGCAATAG+AGG	0.418883	5.3:-42812202	MS.gene053920:CDS
ATGTCAGCATTGGGAAGAAA+TGG	0.423404	5.3:-42812262	MS.gene053920:CDS
GCCAATGAAAGAAGCAATAA+GGG	0.431019	5.3:+42812171	None:intergenic
TAACTTGGTGATGATTGTAT+TGG	0.435126	5.3:-42812507	MS.gene053920:CDS
ATGATTGTATTGGATATGTT+AGG	0.463140	5.3:-42812497	MS.gene053920:CDS
ATACAATCATCACCAAGTTA+TGG	0.469497	5.3:+42812510	None:intergenic
GGAGCATGGTTAATGCGATT+AGG	0.484616	5.3:+42812097	None:intergenic
AGCAACATTCATGTCAGCAT+TGG	0.488005	5.3:-42812272	MS.gene053920:CDS
ACTATGGCTCTGGCATCAGC+TGG	0.494373	5.3:-42812304	MS.gene053920:CDS
GCGATTAGGCTTGAGAAGCT+TGG	0.494528	5.3:+42812111	None:intergenic
GCAACATTCATGTCAGCATT+GGG	0.495269	5.3:-42812271	MS.gene053920:CDS
AAATGCTAGTCTCCATAACT+TGG	0.496794	5.3:-42812522	MS.gene053920:CDS
GGTGAAGTTAACTATGGCTC+TGG	0.500555	5.3:-42812314	MS.gene053920:intron
ATGTTAATCATATCAAGGAC+TGG	0.510976	5.3:+42812075	None:intergenic
TGGTAATTCACATGCAAGAT+GGG	0.512674	5.3:-42812242	MS.gene053920:CDS
ATGGTAATTCACATGCAAGA+TGG	0.522902	5.3:-42812243	MS.gene053920:CDS
ATGGAGACTAGCATTTCCAT+TGG	0.523367	5.3:+42812529	None:intergenic
AGGTTCTTTGTGATAGCCAA+TGG	0.529472	5.3:-42812545	MS.gene053920:CDS
GCTCTGGCATCAGCTGGAGA+TGG	0.540036	5.3:-42812298	MS.gene053920:CDS
CATATCAAGGACTGGGAGCA+TGG	0.559515	5.3:+42812083	None:intergenic
TGTTAATCATATCAAGGACT+GGG	0.560345	5.3:+42812076	None:intergenic
AGGCTTGAGAAGCTTGGCAA+TGG	0.561801	5.3:+42812117	None:intergenic
CTAAAATTGCAATAAATGCA+AGG	0.578209	5.3:+42812447	None:intergenic
AGCTATTGCAATAGAGGTGG+TGG	0.598102	5.3:-42812196	MS.gene053920:CDS
GAAAGCTATTGCAATAGAGG+TGG	0.619990	5.3:-42812199	MS.gene053920:CDS
TCACAGTGATAATCAAGAGA+AGG	0.626568	5.3:+42812143	None:intergenic
AGTCCTTTGTAATCATATTG+GGG	0.628572	5.3:+42812473	None:intergenic
GGACCCCAATATGATTACAA+AGG	0.686673	5.3:-42812476	MS.gene053920:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AGTTTTTTAATTTTTTTCTT+AGG	-	chr5.3:42812247-42812266	MS.gene053920:CDS	10.0%
!!!	ATTTATTGCAATTTTAGACA+TGG	-	chr5.3:42812189-42812208	MS.gene053920:CDS	20.0%
!	AAGTCCTTTGTAATCATATT+GGG	+	chr5.3:42812161-42812180	None:intergenic	25.0%
!	ATGATTGTATTGGATATGTT+AGG	-	chr5.3:42812133-42812152	MS.gene053920:CDS	25.0%
!	CTAAAATTGCAATAAATGCA+AGG	+	chr5.3:42812186-42812205	None:intergenic	25.0%
	AGTCCTTTGTAATCATATTG+GGG	+	chr5.3:42812160-42812179	None:intergenic	30.0%
	ATACAATCATCACCAAGTTA+TGG	+	chr5.3:42812123-42812142	None:intergenic	30.0%
	GAAGTCCTTTGTAATCATAT+TGG	+	chr5.3:42812162-42812181	None:intergenic	30.0%
	TTTGAAAGCTATTGCAATAG+AGG	-	chr5.3:42812428-42812447	MS.gene053920:intron	30.0%
!!	TAACTTGGTGATGATTGTAT+TGG	-	chr5.3:42812123-42812142	MS.gene053920:CDS	30.0%
!!!	TTTTTGGGTGAAGTTAACTA+TGG	-	chr5.3:42812310-42812329	MS.gene053920:CDS	30.0%
	AGCCAATGAAAGAAGCAATA+AGG	+	chr5.3:42812463-42812482	None:intergenic	35.0%
	ATGGTAATTCACATGCAAGA+TGG	-	chr5.3:42812387-42812406	MS.gene053920:intron	35.0%
	GCCAATGAAAGAAGCAATAA+GGG	+	chr5.3:42812462-42812481	None:intergenic	35.0%
	TCACAGTGATAATCAAGAGA+AGG	+	chr5.3:42812490-42812509	None:intergenic	35.0%
	TGGTAATTCACATGCAAGAT+GGG	-	chr5.3:42812388-42812407	MS.gene053920:intron	35.0%
!	AAATGCTAGTCTCCATAACT+TGG	-	chr5.3:42812108-42812127	MS.gene053920:CDS	35.0%
	AGCAACATTCATGTCAGCAT+TGG	-	chr5.3:42812358-42812377	MS.gene053920:intron	40.0%
	ATGGAGACTAGCATTTCCAT+TGG	+	chr5.3:42812104-42812123	None:intergenic	40.0%
	GAAAGCTATTGCAATAGAGG+TGG	-	chr5.3:42812431-42812450	MS.gene053920:intron	40.0%
	GCAACATTCATGTCAGCATT+GGG	-	chr5.3:42812359-42812378	MS.gene053920:intron	40.0%
	GCCCTTATTGCTTCTTTCAT+TGG	-	chr5.3:42812458-42812477	MS.gene053920:CDS	40.0%
	GGACCCCAATATGATTACAA+AGG	-	chr5.3:42812154-42812173	MS.gene053920:CDS	40.0%
!	ATGTCAGCATTGGGAAGAAA+TGG	-	chr5.3:42812368-42812387	MS.gene053920:intron	40.0%
	AGCTATTGCAATAGAGGTGG+TGG	-	chr5.3:42812434-42812453	MS.gene053920:intron	45.0%
	GGAGCATGGTTAATGCGATT+AGG	+	chr5.3:42812536-42812555	None:intergenic	45.0%
	GGTGAAGTTAACTATGGCTC+TGG	-	chr5.3:42812316-42812335	MS.gene053920:intron	45.0%
!!!	TTGATAAATAATATTTTTTT+GGG	-	chr5.3:42812295-42812314	MS.gene053920:CDS	5.0%
!!!	TTTGATAAATAATATTTTTT+TGG	-	chr5.3:42812294-42812313	MS.gene053920:CDS	5.0%
	AGGCTTGAGAAGCTTGGCAA+TGG	+	chr5.3:42812516-42812535	None:intergenic	50.0%
	GCGATTAGGCTTGAGAAGCT+TGG	+	chr5.3:42812522-42812541	None:intergenic	50.0%
!!	ACTATGGCTCTGGCATCAGC+TGG	-	chr5.3:42812326-42812345	MS.gene053920:intron	55.0%
	GCTCTGGCATCAGCTGGAGA+TGG	-	chr5.3:42812332-42812351	MS.gene053920:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	42812087	42812565	42812087	ID=MS.gene053920
chr5.3	mRNA	42812087	42812565	42812087	ID=MS.gene053920.t1;Parent=MS.gene053920
chr5.3	exon	42812442	42812565	42812442	ID=MS.gene053920.t1.exon1;Parent=MS.gene053920.t1
chr5.3	CDS	42812442	42812565	42812442	ID=cds.MS.gene053920.t1;Parent=MS.gene053920.t1
chr5.3	exon	42812087	42812329	42812087	ID=MS.gene053920.t1.exon2;Parent=MS.gene053920.t1
chr5.3	CDS	42812087	42812329	42812087	ID=cds.MS.gene053920.t1;Parent=MS.gene053920.t1

Gene Sequence

>MS.gene053920

GTTCTTTGTGATAGCCAATGGAAATGCTAGTCTCCATAACTTGGTGATGATTGTATTGGATATGTTAGGACCCCAATATGATTACAAAGGACTTCGCCTTGCATTTATTGCAATTTTAGACATGGTAATTTTTTTTAATTAATTTTACATCAATTCTACAAGTTTTTTAATTTTTTTCTTAGGATATCATTTATGTAAGAAAAAATGTTTGATAAATAATATTTTTTTGGGTGAAGTTAACTATGGCTCTGGCATCAGCTGGAGATGGTGCAGCAACATTCATGTCAGCATTGGGAAGAAATGGTAATTCACATGCAAGATGGGATAAGATTTGTGACAAATTTGAAAGCTATTGCAATAGAGGTGGTGGTGCCCTTATTGCTTCTTTCATTGGCTTCATCCTTCTCTTGATTATCACTGTGATGTCCATTGCCAAGCTTCTCAAGCCTAATCGCATTAACCATGCTCCCAGTCCTTGA

Protein sequence

>MS.gene053920.t1

VLCDSQWKCUSPULGDDCIGYVRTPIULQRTSPCIYCNFRHVNYGSGISWRWCSNIHVSIGKKWUFTCKMGUDLUQIUKLLQURWWCPYCFFHWLHPSLDYHCDVHCQASQAUSHUPCSQSL