Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059069.t1 | XP_013443594.1 | 99.1 | 111 | 1 | 0 | 1 | 111 | 53 | 163 | 1.20E-48 | 202.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059069.t1 | B9RW00 | 71.2 | 111 | 32 | 0 | 1 | 111 | 53 | 163 | 7.9e-36 | 151.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059069.t1 | A0A072TJ77 | 99.1 | 111 | 1 | 0 | 1 | 111 | 53 | 163 | 9.0e-49 | 202.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051422 | MS.gene059069 | 0.809384 | 1.94E-50 | -1.69E-46 |
| MS.gene051651 | MS.gene059069 | 0.821845 | 3.25E-53 | -1.69E-46 |
| MS.gene057717 | MS.gene059069 | 0.811045 | 8.52E-51 | -1.69E-46 |
| MS.gene058067 | MS.gene059069 | 0.811752 | 5.98E-51 | -1.69E-46 |
| MS.gene058068 | MS.gene059069 | 0.80172 | 7.88E-49 | -1.69E-46 |
| MS.gene059069 | MS.gene059070 | 0.988023 | 2.03E-172 | -1.69E-46 |
| MS.gene059069 | MS.gene061777 | 0.800283 | 1.55E-48 | -1.69E-46 |
| MS.gene059069 | MS.gene062572 | 0.806942 | 6.44E-50 | -1.69E-46 |
| MS.gene059069 | MS.gene063900 | 0.802094 | 6.60E-49 | -1.69E-46 |
| MS.gene059069 | MS.gene065424 | 0.809504 | 1.83E-50 | -1.69E-46 |
| MS.gene059069 | MS.gene068037 | 0.825668 | 4.13E-54 | -1.69E-46 |
| MS.gene059069 | MS.gene072979 | 0.810839 | 9.44E-51 | -1.69E-46 |
| MS.gene059069 | MS.gene21794 | 0.800345 | 1.51E-48 | -1.69E-46 |
| MS.gene059069 | MS.gene24882 | 0.813002 | 3.19E-51 | -1.69E-46 |
| MS.gene059069 | MS.gene28077 | 0.800518 | 1.39E-48 | -1.69E-46 |
| MS.gene059069 | MS.gene35871 | 0.805949 | 1.04E-49 | -1.69E-46 |
| MS.gene059069 | MS.gene39959 | 0.829446 | 5.12E-55 | -1.69E-46 |
| MS.gene059069 | MS.gene44877 | 0.812219 | 4.73E-51 | -1.69E-46 |
| MS.gene059069 | MS.gene48360 | 0.80966 | 1.70E-50 | -1.69E-46 |
| MS.gene059069 | MS.gene50503 | 0.803881 | 2.82E-49 | -1.69E-46 |
| MS.gene059069 | MS.gene50534 | 0.822444 | 2.36E-53 | -1.69E-46 |
| MS.gene059069 | MS.gene52084 | 0.804305 | 2.30E-49 | -1.69E-46 |
| MS.gene059069 | MS.gene53903 | 0.992559 | 5.05E-194 | -1.69E-46 |
| MS.gene059069 | MS.gene58425 | 0.81475 | 1.31E-51 | -1.69E-46 |
| MS.gene059069 | MS.gene63947 | 0.890472 | 9.55E-74 | -1.69E-46 |
| MS.gene059069 | MS.gene71606 | 0.807388 | 5.18E-50 | -1.69E-46 |
| MS.gene059069 | MS.gene75261 | 0.806422 | 8.29E-50 | -1.69E-46 |
| MS.gene059069 | MS.gene76774 | 0.803372 | 3.60E-49 | -1.69E-46 |
| MS.gene059069 | MS.gene78768 | 0.820922 | 5.31E-53 | -1.69E-46 |
| MS.gene059069 | MS.gene81725 | 0.814176 | 1.76E-51 | -1.69E-46 |
| MS.gene059069 | MS.gene84585 | 0.803628 | 3.18E-49 | -1.69E-46 |
| MS.gene059069 | MS.gene85638 | 0.815331 | 9.77E-52 | -1.69E-46 |
| MS.gene059069 | MS.gene91317 | 0.851277 | 9.85E-61 | -1.69E-46 |
| MS.gene059069 | MS.gene95132 | 0.810332 | 1.21E-50 | -1.69E-46 |
| MS.gene059069 | MS.gene96299 | 0.803253 | 3.81E-49 | -1.69E-46 |
| MS.gene059069 | MS.gene96701 | 0.841642 | 4.20E-58 | -1.69E-46 |
| MS.gene059069 | MS.gene98021 | 0.804835 | 1.79E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTCATGTGATTGCAAAATT+TGG | 0.163683 | 4.2:-60993514 | None:intergenic |
| AACTCTATTGTAACTGTCTA+TGG | 0.255815 | 4.2:+60992536 | MS.gene059069:CDS |
| ACTTGTGGTCGCCATGGATA+TGG | 0.315968 | 4.2:+60992601 | MS.gene059069:CDS |
| GCTTTAGTCGCTAGTTTCAT+TGG | 0.393377 | 4.2:+60993540 | MS.gene059069:CDS |
| CTCAAGCATCTCTGCAGCTT+TGG | 0.400682 | 4.2:+60993425 | MS.gene059069:CDS |
| GTCATGTGATTGCAAAATTT+GGG | 0.430792 | 4.2:-60993513 | None:intergenic |
| CGCCAGAGCAAGCTTTCTGC+AGG | 0.458432 | 4.2:-60992578 | None:intergenic |
| GCTGCTTGGCTACCGATTTG+TGG | 0.463642 | 4.2:+60993483 | MS.gene059069:CDS |
| TTCCTGCAGAAAGCTTGCTC+TGG | 0.469885 | 4.2:+60992576 | MS.gene059069:CDS |
| ATGGAAACAATTATGCTGCT+TGG | 0.504739 | 4.2:+60993469 | MS.gene059069:CDS |
| ATAGCTCAAGTGGCAAAGAA+TGG | 0.508607 | 4.2:+60993450 | MS.gene059069:CDS |
| TTGGGAACTGAACCACAAAT+CGG | 0.511633 | 4.2:-60993495 | None:intergenic |
| AGGGTCAAGGACAGTATGAA+TGG | 0.516263 | 4.2:-60993595 | None:intergenic |
| AAGCTTGCTCTGGCGACTTG+TGG | 0.523109 | 4.2:+60992586 | MS.gene059069:CDS |
| TTCTCAAAAGGAGAGGGTCA+AGG | 0.544463 | 4.2:-60993608 | None:intergenic |
| CTGGCGACTTGTGGTCGCCA+TGG | 0.548545 | 4.2:+60992595 | MS.gene059069:CDS |
| AGCTTTGGCTATAGCTCAAG+TGG | 0.581117 | 4.2:+60993440 | MS.gene059069:CDS |
| TTAGGACATTACCATATCCA+TGG | 0.585943 | 4.2:-60992612 | None:intergenic |
| AGCTTTCTGCAGGAAGAAAG+AGG | 0.586262 | 4.2:-60992568 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGAAATGCTAAATTATTA+TGG | + | chr4.2:60993333-60993352 | MS.gene059069:intron | 15.0% |
| !!! | ATTTTATATGATCAAACTTA+GGG | - | chr4.2:60993124-60993143 | None:intergenic | 15.0% |
| !!! | GTTTTATTTTTATGGATTTA+AGG | - | chr4.2:60993054-60993073 | None:intergenic | 15.0% |
| !!! | TATTTTATATGATCAAACTT+AGG | - | chr4.2:60993125-60993144 | None:intergenic | 15.0% |
| !! | AAGAACAAATGTTTCAAATT+TGG | - | chr4.2:60992726-60992745 | None:intergenic | 20.0% |
| !! | ATGCTAAATTATTATGGTTA+TGG | + | chr4.2:60993339-60993358 | MS.gene059069:intron | 20.0% |
| !! | CAAACATTATATGACTTAAA+TGG | - | chr4.2:60992856-60992875 | None:intergenic | 20.0% |
| !!! | ATGAGAAGGTTTTATTTTTA+TGG | - | chr4.2:60993062-60993081 | None:intergenic | 20.0% |
| !!! | TATTTTTATGGATTTAAGGT+TGG | - | chr4.2:60993050-60993069 | None:intergenic | 20.0% |
| !!! | TGTTCTTCATGTTTTTAAAA+TGG | + | chr4.2:60992739-60992758 | MS.gene059069:intron | 20.0% |
| !!! | TTTAATTGATTTTTATGAGC+AGG | + | chr4.2:60993383-60993402 | MS.gene059069:intron | 20.0% |
| !!! | TTTTCGTTTTTTCATTTCAT+TGG | - | chr4.2:60993315-60993334 | None:intergenic | 20.0% |
| ! | ATGATAAAGAGGTTAACTTT+AGG | - | chr4.2:60992633-60992652 | None:intergenic | 25.0% |
| ! | ATTAGAATACTAGTTATGTC+TGG | + | chr4.2:60993169-60993188 | MS.gene059069:intron | 25.0% |
| ! | GACATAACTAGTATTCTAAT+AGG | - | chr4.2:60993169-60993188 | None:intergenic | 25.0% |
| ! | TAAGTCATATAATGTTTGCA+TGG | + | chr4.2:60992858-60992877 | MS.gene059069:intron | 25.0% |
| ! | TATGAACTCTTTAACATAGT+TGG | - | chr4.2:60992898-60992917 | None:intergenic | 25.0% |
| ! | TATGTTAAAGAGTTCATACA+TGG | + | chr4.2:60992900-60992919 | MS.gene059069:intron | 25.0% |
| !! | CTAACCAATGTATGATTTTT+CGG | + | chr4.2:60993237-60993256 | MS.gene059069:intron | 25.0% |
| !!! | TCTTCATGTTTTTAAAATGG+AGG | + | chr4.2:60992742-60992761 | MS.gene059069:intron | 25.0% |
| AAAGTTCTTGCATGATAAAG+AGG | - | chr4.2:60992644-60992663 | None:intergenic | 30.0% | |
| AACACGTCAAACAATAAACT+TGG | - | chr4.2:60993276-60993295 | None:intergenic | 30.0% | |
| AACTCTATTGTAACTGTCTA+TGG | + | chr4.2:60992536-60992555 | MS.gene059069:CDS | 30.0% | |
| AACTCTTTAACATAGTTGGA+TGG | - | chr4.2:60992894-60992913 | None:intergenic | 30.0% | |
| GTCATGTGATTGCAAAATTT+GGG | - | chr4.2:60993516-60993535 | None:intergenic | 30.0% | |
| TGTCATGTGATTGCAAAATT+TGG | - | chr4.2:60993517-60993536 | None:intergenic | 30.0% | |
| ! | AAATTTTGCAATCACATGAC+AGG | + | chr4.2:60993516-60993535 | MS.gene059069:CDS | 30.0% |
| ! | AAGTTTTTCTCAAAAGGAGA+GGG | - | chr4.2:60993617-60993636 | None:intergenic | 30.0% |
| ! | GCATTTTTGTTAGTTGAGTT+AGG | + | chr4.2:60992811-60992830 | MS.gene059069:intron | 30.0% |
| ! | TGTAACTGTCTATGGATTTT+TGG | + | chr4.2:60992544-60992563 | MS.gene059069:CDS | 30.0% |
| !!! | TGTTTTTAAAATGGAGGTGT+TGG | + | chr4.2:60992748-60992767 | MS.gene059069:intron | 30.0% |
| ATGGAAACAATTATGCTGCT+TGG | + | chr4.2:60993469-60993488 | MS.gene059069:CDS | 35.0% | |
| GAATACTAGTTATGTCTGGA+TGG | + | chr4.2:60993173-60993192 | MS.gene059069:intron | 35.0% | |
| GTAAAGTCAGACGTTATGAT+CGG | - | chr4.2:60992784-60992803 | None:intergenic | 35.0% | |
| TAAAGTCAGACGTTATGATC+GGG | - | chr4.2:60992783-60992802 | None:intergenic | 35.0% | |
| TCAGCCGAAAAATCATACAT+TGG | - | chr4.2:60993244-60993263 | None:intergenic | 35.0% | |
| TGGATGGAGATTAAGTCTAT+AGG | + | chr4.2:60993189-60993208 | MS.gene059069:intron | 35.0% | |
| TTAGGACATTACCATATCCA+TGG | - | chr4.2:60992615-60992634 | None:intergenic | 35.0% | |
| ! | ACATTGGTTAGTCACACATT+GGG | - | chr4.2:60993228-60993247 | None:intergenic | 35.0% |
| ! | CAAGTTTTTCTCAAAAGGAG+AGG | - | chr4.2:60993618-60993637 | None:intergenic | 35.0% |
| ! | TACATTGGTTAGTCACACAT+TGG | - | chr4.2:60993229-60993248 | None:intergenic | 35.0% |
| !! | TATGGATTTAAGGTTGGTGA+TGG | - | chr4.2:60993044-60993063 | None:intergenic | 35.0% |
| AAAGTCAGACGTTATGATCG+GGG | - | chr4.2:60992782-60992801 | None:intergenic | 40.0% | |
| ATAGCTCAAGTGGCAAAGAA+TGG | + | chr4.2:60993450-60993469 | MS.gene059069:CDS | 40.0% | |
| TTGGGAACTGAACCACAAAT+CGG | - | chr4.2:60993498-60993517 | None:intergenic | 40.0% | |
| ! | GCTTTAGTCGCTAGTTTCAT+TGG | + | chr4.2:60993540-60993559 | MS.gene059069:CDS | 40.0% |
| AGCGTGTTTGAGAGATGAGA+AGG | - | chr4.2:60993076-60993095 | None:intergenic | 45.0% | |
| AGCTTTCTGCAGGAAGAAAG+AGG | - | chr4.2:60992571-60992590 | None:intergenic | 45.0% | |
| AGGGTCAAGGACAGTATGAA+TGG | - | chr4.2:60993598-60993617 | None:intergenic | 45.0% | |
| TTCTCAAAAGGAGAGGGTCA+AGG | - | chr4.2:60993611-60993630 | None:intergenic | 45.0% | |
| ! | AGCTTTGGCTATAGCTCAAG+TGG | + | chr4.2:60993440-60993459 | MS.gene059069:CDS | 45.0% |
| ACTTGTGGTCGCCATGGATA+TGG | + | chr4.2:60992601-60992620 | MS.gene059069:CDS | 50.0% | |
| CTCAAGCATCTCTGCAGCTT+TGG | + | chr4.2:60993425-60993444 | MS.gene059069:CDS | 50.0% | |
| ! | TTCCTGCAGAAAGCTTGCTC+TGG | + | chr4.2:60992576-60992595 | MS.gene059069:CDS | 50.0% |
| ! | AAGCTTGCTCTGGCGACTTG+TGG | + | chr4.2:60992586-60992605 | MS.gene059069:CDS | 55.0% |
| !! | GCTGCTTGGCTACCGATTTG+TGG | + | chr4.2:60993483-60993502 | MS.gene059069:CDS | 55.0% |
| CGCCAGAGCAAGCTTTCTGC+AGG | - | chr4.2:60992581-60992600 | None:intergenic | 60.0% | |
| CTGGCGACTTGTGGTCGCCA+TGG | + | chr4.2:60992595-60992614 | MS.gene059069:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 60992520 | 60993638 | 60992520 | ID=MS.gene059069 |
| chr4.2 | mRNA | 60992520 | 60993638 | 60992520 | ID=MS.gene059069.t1;Parent=MS.gene059069 |
| chr4.2 | exon | 60992520 | 60992622 | 60992520 | ID=MS.gene059069.t1.exon1;Parent=MS.gene059069.t1 |
| chr4.2 | CDS | 60992520 | 60992622 | 60992520 | ID=cds.MS.gene059069.t1;Parent=MS.gene059069.t1 |
| chr4.2 | exon | 60993405 | 60993638 | 60993405 | ID=MS.gene059069.t1.exon2;Parent=MS.gene059069.t1 |
| chr4.2 | CDS | 60993405 | 60993638 | 60993405 | ID=cds.MS.gene059069.t1;Parent=MS.gene059069.t1 |
| Gene Sequence |
| Protein sequence |
