Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51156.t1 | PNX77387.1 | 78.7 | 207 | 44 | 0 | 1 | 207 | 13 | 219 | 8.80E-88 | 333.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51156.t1 | A0A2K3LFT9 | 78.7 | 207 | 44 | 0 | 1 | 207 | 13 | 219 | 6.4e-88 | 333.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene51156 | 0.81292 | 3.33E-51 | -1.69E-46 |
| MS.gene050780 | MS.gene51156 | 0.808109 | 3.64E-50 | -1.69E-46 |
| MS.gene052384 | MS.gene51156 | 0.81432 | 1.64E-51 | -1.69E-46 |
| MS.gene052896 | MS.gene51156 | 0.816355 | 5.77E-52 | -1.69E-46 |
| MS.gene054236 | MS.gene51156 | 0.810955 | 8.91E-51 | -1.69E-46 |
| MS.gene05468 | MS.gene51156 | 0.814591 | 1.42E-51 | -1.69E-46 |
| MS.gene055701 | MS.gene51156 | 0.803324 | 3.68E-49 | -1.69E-46 |
| MS.gene05761 | MS.gene51156 | 0.803201 | 3.90E-49 | -1.69E-46 |
| MS.gene058146 | MS.gene51156 | 0.802013 | 6.86E-49 | -1.69E-46 |
| MS.gene060802 | MS.gene51156 | 0.805809 | 1.12E-49 | -1.69E-46 |
| MS.gene060803 | MS.gene51156 | 0.814697 | 1.35E-51 | -1.69E-46 |
| MS.gene060998 | MS.gene51156 | 0.823714 | 1.19E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51156.t1 | MTR_8g090105 | 71.429 | 210 | 59 | 1 | 1 | 210 | 58 | 266 | 2.39e-104 | 301 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51156.t1 | AT3G57360 | 46.569 | 204 | 100 | 4 | 1 | 203 | 50 | 245 | 2.28e-56 | 179 |
| MS.gene51156.t1 | AT3G02370 | 44.828 | 203 | 105 | 3 | 3 | 204 | 1 | 197 | 2.52e-54 | 172 |
| MS.gene51156.t1 | AT3G02370 | 44.828 | 203 | 105 | 3 | 3 | 204 | 1 | 197 | 2.52e-54 | 172 |
| MS.gene51156.t1 | AT3G02370 | 44.828 | 203 | 105 | 3 | 3 | 204 | 1 | 197 | 2.52e-54 | 172 |
| MS.gene51156.t1 | AT3G02370 | 48.171 | 164 | 79 | 2 | 3 | 165 | 1 | 159 | 8.50e-48 | 154 |
Find 37 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCAAGATCAGAAGATTTA+AGG | 0.117924 | 7.3:-27328477 | None:intergenic |
| CATTCCATTGATAAGTTATT+TGG | 0.155844 | 7.3:+27328547 | MS.gene51156:CDS |
| CACAAGCACATGAGAAAATT+TGG | 0.207694 | 7.3:-27328652 | None:intergenic |
| GATTTGGCACAGGTATCTTA+TGG | 0.264509 | 7.3:+27328264 | MS.gene51156:intron |
| AAAGTTGATGGTCGGAAGTT+TGG | 0.269564 | 7.3:+27328352 | MS.gene51156:CDS |
| TTCAAGATCAGAAGATTTAA+GGG | 0.330837 | 7.3:-27328476 | None:intergenic |
| CTTAAATCTTCTGATCTTGA+AGG | 0.344176 | 7.3:+27328478 | MS.gene51156:CDS |
| GTAGCTCTTGAGAGAAAATA+TGG | 0.344246 | 7.3:+27329199 | MS.gene51156:CDS |
| ACCATTATCTAATAGATCTA+AGG | 0.353230 | 7.3:-27328296 | None:intergenic |
| GGAAGATAAACATCAAAATC+TGG | 0.353821 | 7.3:-27328595 | None:intergenic |
| AAGCAGACAATTAGTAGATA+TGG | 0.368214 | 7.3:+27328510 | MS.gene51156:CDS |
| GACTTTCTAAACCTCTTGTT+TGG | 0.373355 | 7.3:-27328616 | None:intergenic |
| GCCTTAGATCTATTAGATAA+TGG | 0.413209 | 7.3:+27328295 | MS.gene51156:CDS |
| TTTGCGAGGTCACTGATAGA+AGG | 0.426366 | 7.3:+27329239 | MS.gene51156:CDS |
| GCTACAATGTCTGCATTAGA+TGG | 0.433477 | 7.3:-27329181 | None:intergenic |
| GTCGGAAGTTTGGATGTTGT+TGG | 0.440117 | 7.3:+27328362 | MS.gene51156:CDS |
| TTCGGCGGCATGGTGTTGCT+TGG | 0.440523 | 7.3:+27328431 | MS.gene51156:CDS |
| AGTCTCTTGGCTACATAGTT+CGG | 0.448906 | 7.3:+27328413 | MS.gene51156:CDS |
| CAAGATTTACACTTCAATTG+AGG | 0.454593 | 7.3:+27328008 | MS.gene51156:CDS |
| GTCACCAAATAACTTATCAA+TGG | 0.481135 | 7.3:-27328551 | None:intergenic |
| TCGGAAGTTTGGATGTTGTT+GGG | 0.490686 | 7.3:+27328363 | MS.gene51156:CDS |
| GCAGAAGTGTATGAAAAGAA+AGG | 0.520348 | 7.3:+27327949 | MS.gene51156:CDS |
| GGCTACATAGTTCGGCGGCA+TGG | 0.523179 | 7.3:+27328421 | MS.gene51156:CDS |
| AGGTTTAGAAAGTCTTCTCC+TGG | 0.525155 | 7.3:+27328625 | MS.gene51156:CDS |
| TATAGGCACCTCAAGTCTCT+TGG | 0.537490 | 7.3:+27328400 | MS.gene51156:CDS |
| ATGTTTATCTTCCAAACAAG+AGG | 0.541371 | 7.3:+27328605 | MS.gene51156:CDS |
| ATGAGAAAATTTGGAATACC+AGG | 0.550816 | 7.3:-27328643 | None:intergenic |
| ACAACTGGAATAGTTCGTAA+TGG | 0.568611 | 7.3:+27327985 | MS.gene51156:CDS |
| ATGGAATGAAGAGATGGGAA+TGG | 0.574705 | 7.3:+27327927 | None:intergenic |
| TAGATGTTCATCCTTGTCAA+AGG | 0.597660 | 7.3:-27329275 | None:intergenic |
| ATGTCTGCATTAGATGGAGG+AGG | 0.599739 | 7.3:-27329175 | None:intergenic |
| CATTCAATTGAAAATTTGCG+AGG | 0.600948 | 7.3:+27329225 | MS.gene51156:CDS |
| CTCTTGGCTACATAGTTCGG+CGG | 0.631908 | 7.3:+27328416 | MS.gene51156:CDS |
| CTATGTAGCCAAGAGACTTG+AGG | 0.654136 | 7.3:-27328408 | None:intergenic |
| ACAATGTCTGCATTAGATGG+AGG | 0.656582 | 7.3:-27329178 | None:intergenic |
| ATCAATGGAATGCTTGACTG+TGG | 0.707373 | 7.3:-27328536 | None:intergenic |
| GCTCTTGAGAGAAAATATGG+TGG | 0.725754 | 7.3:+27329202 | MS.gene51156:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACAAACAAATTATTTAGTT+AGG | + | chr7.3:27328135-27328154 | MS.gene51156:intron | 15.0% |
| !!! | ATTATTATGAATTCCTTTTT+CGG | - | chr7.3:27328195-27328214 | None:intergenic | 15.0% |
| !!! | TTTTGGAAAATCTATTTAAT+CGG | + | chr7.3:27329125-27329144 | MS.gene51156:intron | 15.0% |
| !! | AGTAAAAAATGAAAGAATGT+TGG | - | chr7.3:27329094-27329113 | None:intergenic | 20.0% |
| !! | GAAATGTATAAAAAAGTTGA+TGG | + | chr7.3:27328340-27328359 | MS.gene51156:CDS | 20.0% |
| !! | TCTTAATGAAAAGAAATGAA+AGG | - | chr7.3:27328936-27328955 | None:intergenic | 20.0% |
| !!! | CATAATAATACATTTTTGCT+AGG | + | chr7.3:27328206-27328225 | MS.gene51156:intron | 20.0% |
| ! | AAATGCCTAATAATTACTCA+AGG | - | chr7.3:27328903-27328922 | None:intergenic | 25.0% |
| ! | ACCATTATCTAATAGATCTA+AGG | - | chr7.3:27328299-27328318 | None:intergenic | 25.0% |
| ! | ATGAAAAGAAAGGAAAAATG+TGG | + | chr7.3:27327959-27327978 | MS.gene51156:CDS | 25.0% |
| ! | ATTGCATTTGTAATCTGTTA+GGG | + | chr7.3:27329150-27329169 | MS.gene51156:intron | 25.0% |
| ! | CACATAAATTACCATAGAAA+TGG | - | chr7.3:27328869-27328888 | None:intergenic | 25.0% |
| ! | CATTCCATTGATAAGTTATT+TGG | + | chr7.3:27328547-27328566 | MS.gene51156:CDS | 25.0% |
| ! | TGAAAAGAAAGGAAAAATGT+GGG | + | chr7.3:27327960-27327979 | MS.gene51156:CDS | 25.0% |
| ! | TTCAAGATCAGAAGATTTAA+GGG | - | chr7.3:27328479-27328498 | None:intergenic | 25.0% |
| !! | GATTTTCCAAAACAAGATAA+AGG | - | chr7.3:27329117-27329136 | None:intergenic | 25.0% |
| !! | TGAGTAATTATTAGGCATTT+TGG | + | chr7.3:27328903-27328922 | MS.gene51156:intron | 25.0% |
| !!! | TAGGATAAAGTTTTGAAAAC+TGG | + | chr7.3:27328225-27328244 | MS.gene51156:intron | 25.0% |
| !!! | TGCATATGTGAATTTTGATT+TGG | + | chr7.3:27328248-27328267 | MS.gene51156:intron | 25.0% |
| !!! | TGTATAAAAAAGTTGATGGT+CGG | + | chr7.3:27328344-27328363 | MS.gene51156:CDS | 25.0% |
| !!! | TTTACTCCTTTATCTTGTTT+TGG | + | chr7.3:27329108-27329127 | MS.gene51156:intron | 25.0% |
| AAGCAGACAATTAGTAGATA+TGG | + | chr7.3:27328510-27328529 | MS.gene51156:CDS | 30.0% | |
| ATGTTTATCTTCCAAACAAG+AGG | + | chr7.3:27328605-27328624 | MS.gene51156:CDS | 30.0% | |
| CAAGATTTACACTTCAATTG+AGG | + | chr7.3:27328008-27328027 | MS.gene51156:CDS | 30.0% | |
| CATTCAATTGAAAATTTGCG+AGG | + | chr7.3:27329225-27329244 | MS.gene51156:CDS | 30.0% | |
| CATTGCATTTGTAATCTGTT+AGG | + | chr7.3:27329149-27329168 | MS.gene51156:intron | 30.0% | |
| CTTAAATCTTCTGATCTTGA+AGG | + | chr7.3:27328478-27328497 | MS.gene51156:CDS | 30.0% | |
| CTTCAAGATCAGAAGATTTA+AGG | - | chr7.3:27328480-27328499 | None:intergenic | 30.0% | |
| GACTTCCTTGAGTAATTATT+AGG | + | chr7.3:27328895-27328914 | MS.gene51156:intron | 30.0% | |
| GCCTTAGATCTATTAGATAA+TGG | + | chr7.3:27328295-27328314 | MS.gene51156:CDS | 30.0% | |
| GGAAGATAAACATCAAAATC+TGG | - | chr7.3:27328598-27328617 | None:intergenic | 30.0% | |
| GTCACCAAATAACTTATCAA+TGG | - | chr7.3:27328554-27328573 | None:intergenic | 30.0% | |
| TAAATTGTAATTTCGTAGCG+TGG | - | chr7.3:27328813-27328832 | None:intergenic | 30.0% | |
| TGGTAAAATTTCACAACAGA+AGG | - | chr7.3:27328075-27328094 | None:intergenic | 30.0% | |
| TTGCATTTGTAATCTGTTAG+GGG | + | chr7.3:27329151-27329170 | MS.gene51156:intron | 30.0% | |
| ! | AAGTTTCTTTTCCTTTGACA+AGG | + | chr7.3:27329264-27329283 | MS.gene51156:CDS | 30.0% |
| ! | AGATACAATTGCCATTTCTA+TGG | + | chr7.3:27328855-27328874 | MS.gene51156:intron | 30.0% |
| ! | ATGAGAAAATTTGGAATACC+AGG | - | chr7.3:27328646-27328665 | None:intergenic | 30.0% |
| ! | GTTGTGAAATTTTACCAGTA+GGG | + | chr7.3:27328078-27328097 | MS.gene51156:intron | 30.0% |
| ! | TGTTGTGAAATTTTACCAGT+AGG | + | chr7.3:27328077-27328096 | MS.gene51156:intron | 30.0% |
| ! | TTTTCATTAAGAGTGATGTG+TGG | + | chr7.3:27328944-27328963 | MS.gene51156:intron | 30.0% |
| ACAACTGGAATAGTTCGTAA+TGG | + | chr7.3:27327985-27328004 | MS.gene51156:CDS | 35.0% | |
| ATGAAAGAATGTTGGAGCTA+CGG | - | chr7.3:27329086-27329105 | None:intergenic | 35.0% | |
| CACAAGCACATGAGAAAATT+TGG | - | chr7.3:27328655-27328674 | None:intergenic | 35.0% | |
| GACTTTCTAAACCTCTTGTT+TGG | - | chr7.3:27328619-27328638 | None:intergenic | 35.0% | |
| GATGCAAAATTAGCCGAAAA+AGG | + | chr7.3:27328179-27328198 | MS.gene51156:intron | 35.0% | |
| GTAGCTCTTGAGAGAAAATA+TGG | + | chr7.3:27329199-27329218 | MS.gene51156:CDS | 35.0% | |
| TAGATGTTCATCCTTGTCAA+AGG | - | chr7.3:27329278-27329297 | None:intergenic | 35.0% | |
| ! | ATGTGTGGAGTCTATCTTTT+TGG | + | chr7.3:27328959-27328978 | MS.gene51156:intron | 35.0% |
| ! | ATTTTCTCATGTGCTTGTGT+AGG | + | chr7.3:27328656-27328675 | MS.gene51156:CDS | 35.0% |
| ! | GCAGAAGTGTATGAAAAGAA+AGG | + | chr7.3:27327949-27327968 | MS.gene51156:CDS | 35.0% |
| ! | TCTATCTTTTTGGACCACTT+TGG | + | chr7.3:27328969-27328988 | MS.gene51156:intron | 35.0% |
| !!! | TGTGAATTTTGATTTGGCAC+AGG | + | chr7.3:27328254-27328273 | MS.gene51156:intron | 35.0% |
| AAACGACTGAAAAATCAGCG+TGG | - | chr7.3:27329038-27329057 | None:intergenic | 40.0% | |
| ACAATGTCTGCATTAGATGG+AGG | - | chr7.3:27329181-27329200 | None:intergenic | 40.0% | |
| AGGTTTAGAAAGTCTTCTCC+TGG | + | chr7.3:27328625-27328644 | MS.gene51156:CDS | 40.0% | |
| AGTCTCTTGGCTACATAGTT+CGG | + | chr7.3:27328413-27328432 | MS.gene51156:CDS | 40.0% | |
| ATCAATGGAATGCTTGACTG+TGG | - | chr7.3:27328539-27328558 | None:intergenic | 40.0% | |
| GATTTGGCACAGGTATCTTA+TGG | + | chr7.3:27328264-27328283 | MS.gene51156:intron | 40.0% | |
| GCTACAATGTCTGCATTAGA+TGG | - | chr7.3:27329184-27329203 | None:intergenic | 40.0% | |
| GCTCTTGAGAGAAAATATGG+TGG | + | chr7.3:27329202-27329221 | MS.gene51156:CDS | 40.0% | |
| GGAAAAATGTGGGTAACAAC+TGG | + | chr7.3:27327970-27327989 | MS.gene51156:CDS | 40.0% | |
| TCGGAAGTTTGGATGTTGTT+GGG | + | chr7.3:27328363-27328382 | MS.gene51156:CDS | 40.0% | |
| TGCACATAATGTGTCCAAAG+TGG | - | chr7.3:27328986-27329005 | None:intergenic | 40.0% | |
| ! | ATGTTGTTGGGAGCTTTTTG+AGG | + | chr7.3:27328375-27328394 | MS.gene51156:CDS | 40.0% |
| !! | AAAGTTGATGGTCGGAAGTT+TGG | + | chr7.3:27328352-27328371 | MS.gene51156:CDS | 40.0% |
| ATGTCTGCATTAGATGGAGG+AGG | - | chr7.3:27329178-27329197 | None:intergenic | 45.0% | |
| CTATGTAGCCAAGAGACTTG+AGG | - | chr7.3:27328411-27328430 | None:intergenic | 45.0% | |
| GTCGGAAGTTTGGATGTTGT+TGG | + | chr7.3:27328362-27328381 | MS.gene51156:CDS | 45.0% | |
| TATAGGCACCTCAAGTCTCT+TGG | + | chr7.3:27328400-27328419 | MS.gene51156:CDS | 45.0% | |
| TTTGCGAGGTCACTGATAGA+AGG | + | chr7.3:27329239-27329258 | MS.gene51156:CDS | 45.0% | |
| ! | GTGCTGTGAATTTTCCCTAC+TGG | - | chr7.3:27328095-27328114 | None:intergenic | 45.0% |
| !!! | GGGAGCTTTTTGAGGCTTAT+AGG | + | chr7.3:27328383-27328402 | MS.gene51156:CDS | 45.0% |
| CTCTTGGCTACATAGTTCGG+CGG | + | chr7.3:27328416-27328435 | MS.gene51156:CDS | 50.0% | |
| GGCTACATAGTTCGGCGGCA+TGG | + | chr7.3:27328421-27328440 | MS.gene51156:CDS | 60.0% | |
| !! | TTCGGCGGCATGGTGTTGCT+TGG | + | chr7.3:27328431-27328450 | MS.gene51156:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 27327940 | 27329303 | 27327940 | ID=MS.gene51156 |
| chr7.3 | mRNA | 27327940 | 27329303 | 27327940 | ID=MS.gene51156.t1;Parent=MS.gene51156 |
| chr7.3 | exon | 27327940 | 27328037 | 27327940 | ID=MS.gene51156.t1.exon1;Parent=MS.gene51156.t1 |
| chr7.3 | CDS | 27327940 | 27328037 | 27327940 | ID=cds.MS.gene51156.t1;Parent=MS.gene51156.t1 |
| chr7.3 | exon | 27328276 | 27328677 | 27328276 | ID=MS.gene51156.t1.exon2;Parent=MS.gene51156.t1 |
| chr7.3 | CDS | 27328276 | 27328677 | 27328276 | ID=cds.MS.gene51156.t1;Parent=MS.gene51156.t1 |
| chr7.3 | exon | 27329171 | 27329303 | 27329171 | ID=MS.gene51156.t1.exon3;Parent=MS.gene51156.t1 |
| chr7.3 | CDS | 27329171 | 27329303 | 27329171 | ID=cds.MS.gene51156.t1;Parent=MS.gene51156.t1 |
| Gene Sequence |
| Protein sequence |
