Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52076.t1 | RHN61505.1 | 97.3 | 187 | 5 | 0 | 1 | 187 | 1 | 187 | 1.80E-74 | 288.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52076.t1 | Q9C9Z8 | 59.1 | 186 | 76 | 0 | 1 | 186 | 1 | 186 | 2.1e-52 | 206.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52076.t1 | A0A072UXC1 | 97.3 | 187 | 5 | 0 | 1 | 187 | 1 | 187 | 1.3e-74 | 288.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene21642 | MS.gene52076 | PPI |
| MS.gene22330 | MS.gene52076 | PPI |
| MS.gene072752 | MS.gene52076 | PPI |
| MS.gene06042 | MS.gene52076 | PPI |
| MS.gene55771 | MS.gene52076 | PPI |
| MS.gene52076 | MS.gene06042 | PPI |
| MS.gene048900 | MS.gene52076 | PPI |
| MS.gene91075 | MS.gene52076 | PPI |
| MS.gene024661 | MS.gene52076 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52076.t1 | MTR_4g073800 | 96.809 | 188 | 6 | 0 | 1 | 188 | 1 | 188 | 3.28e-127 | 358 |
| MS.gene52076.t1 | MTR_5g009720 | 68.750 | 192 | 59 | 1 | 1 | 191 | 1 | 192 | 1.27e-88 | 260 |
| MS.gene52076.t1 | MTR_8g099190 | 63.184 | 201 | 62 | 2 | 1 | 191 | 1 | 199 | 1.95e-84 | 249 |
| MS.gene52076.t1 | MTR_8g099190 | 63.184 | 201 | 58 | 3 | 1 | 189 | 1 | 197 | 2.59e-83 | 247 |
| MS.gene52076.t1 | MTR_8g099190 | 63.500 | 200 | 57 | 3 | 1 | 188 | 1 | 196 | 4.10e-83 | 246 |
| MS.gene52076.t1 | MTR_8g099190 | 57.000 | 200 | 55 | 4 | 1 | 188 | 1 | 181 | 1.40e-68 | 209 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52076.t1 | AT4G11150 | 70.000 | 180 | 54 | 0 | 1 | 180 | 1 | 180 | 8.00e-88 | 258 |
| MS.gene52076.t1 | AT3G08560 | 65.957 | 188 | 64 | 0 | 1 | 188 | 1 | 188 | 1.78e-84 | 249 |
| MS.gene52076.t1 | AT1G64200 | 63.918 | 194 | 62 | 2 | 1 | 188 | 1 | 192 | 3.07e-82 | 242 |
| MS.gene52076.t1 | AT1G64200 | 65.591 | 186 | 58 | 1 | 1 | 180 | 1 | 186 | 1.33e-81 | 242 |
| MS.gene52076.t1 | AT1G64200 | 65.591 | 186 | 58 | 1 | 1 | 180 | 1 | 186 | 1.45e-81 | 242 |
Find 30 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGCTTTCTCCTCAGCTTCT+TGG | 0.251565 | 4.1:-29958687 | None:intergenic |
| CGATCATCAAGGGCAATCTT+AGG | 0.255958 | 4.1:-29965971 | None:intergenic |
| TATGAAAGAGGATGCTAAAA+AGG | 0.275670 | 4.1:+29959338 | MS.gene52076:CDS |
| AATAGAAGGATATACCAAAA+AGG | 0.385736 | 4.1:-29966043 | None:intergenic |
| CATCAAAGAAATAATTGTTC+AGG | 0.393403 | 4.1:+29959404 | MS.gene52076:CDS |
| GCTGAATGCATCTCGTATAA+AGG | 0.394063 | 4.1:+29959284 | MS.gene52076:CDS |
| TCGACGAACATCAATCTGTT+TGG | 0.411208 | 4.1:-29959081 | None:intergenic |
| GATCATCAAGGGCAATCTTA+GGG | 0.420043 | 4.1:-29965970 | None:intergenic |
| AGCAAATCCAGCAGATGATT+AGG | 0.425110 | 4.1:+29958656 | MS.gene52076:CDS |
| TCACCACCTCATCTTGTGCT+TGG | 0.471251 | 4.1:-29959311 | None:intergenic |
| TTTAATCAAAGATAAATAGA+AGG | 0.481719 | 4.1:-29966057 | None:intergenic |
| CTTTCTCCTCAGCTTCTTGG+CGG | 0.494892 | 4.1:-29958684 | None:intergenic |
| TGAAATATCTGTTGCTGCAG+AGG | 0.507699 | 4.1:+29958711 | MS.gene52076:CDS |
| GGATGAACCTAATCATCTGC+TGG | 0.525749 | 4.1:-29958663 | None:intergenic |
| GGAGATAAACACGATCATCA+AGG | 0.564384 | 4.1:-29965982 | None:intergenic |
| GCTTGTTGAGTCGTTGCTAG+AGG | 0.584576 | 4.1:+29965907 | MS.gene52076:CDS |
| CAGACAAGAGTACGAGCGAA+AGG | 0.590203 | 4.1:+29959058 | MS.gene52076:CDS |
| CTACATCGTAGTAGCACAGA+TGG | 0.593942 | 4.1:-29965869 | None:intergenic |
| GTAGCACAGATGGTTCCCTC+AGG | 0.603951 | 4.1:-29965859 | None:intergenic |
| GGTGGTGAATTCTATGAAAG+AGG | 0.606757 | 4.1:+29959326 | MS.gene52076:CDS |
| TGTAGGGTTTACTACGCCTG+AGG | 0.609492 | 4.1:+29965843 | MS.gene52076:intron |
| GAGATAAACACGATCATCAA+GGG | 0.613062 | 4.1:-29965981 | None:intergenic |
| ATCATCAAGGGCAATCTTAG+GGG | 0.623318 | 4.1:-29965969 | None:intergenic |
| GTTCATCCGCCAAGAAGCTG+AGG | 0.630671 | 4.1:+29958678 | MS.gene52076:CDS |
| TCATCAAGGGCAATCTTAGG+GGG | 0.635912 | 4.1:-29965968 | None:intergenic |
| GGTACTCCAAGCACAAGATG+AGG | 0.637147 | 4.1:+29959305 | MS.gene52076:CDS |
| TGTGCTACTACGATGTAGAG+AGG | 0.638418 | 4.1:+29965874 | MS.gene52076:CDS |
| GTGCTACTACGATGTAGAGA+GGG | 0.653633 | 4.1:+29965875 | MS.gene52076:CDS |
| GTAGGGTTTACTACGCCTGA+GGG | 0.660356 | 4.1:+29965844 | MS.gene52076:intron |
| ACTCCAAGCACAAGATGAGG+TGG | 0.665263 | 4.1:+29959308 | MS.gene52076:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 29958634 | 29966078 | 29958634 | ID=MS.gene52076 |
| chr4.1 | mRNA | 29958634 | 29966078 | 29958634 | ID=MS.gene52076.t1;Parent=MS.gene52076 |
| chr4.1 | exon | 29958634 | 29958735 | 29958634 | ID=MS.gene52076.t1.exon1;Parent=MS.gene52076.t1 |
| chr4.1 | CDS | 29958634 | 29958735 | 29958634 | ID=cds.MS.gene52076.t1;Parent=MS.gene52076.t1 |
| chr4.1 | exon | 29959008 | 29959108 | 29959008 | ID=MS.gene52076.t1.exon2;Parent=MS.gene52076.t1 |
| chr4.1 | CDS | 29959008 | 29959108 | 29959008 | ID=cds.MS.gene52076.t1;Parent=MS.gene52076.t1 |
| chr4.1 | exon | 29959269 | 29959425 | 29959269 | ID=MS.gene52076.t1.exon3;Parent=MS.gene52076.t1 |
| chr4.1 | CDS | 29959269 | 29959425 | 29959269 | ID=cds.MS.gene52076.t1;Parent=MS.gene52076.t1 |
| chr4.1 | exon | 29965848 | 29966078 | 29965848 | ID=MS.gene52076.t1.exon4;Parent=MS.gene52076.t1 |
| chr4.1 | CDS | 29965848 | 29966078 | 29965848 | ID=cds.MS.gene52076.t1;Parent=MS.gene52076.t1 |
| Gene Sequence |
| Protein sequence |
