AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53091

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53091.t1 MTR_5g042590 97.642 1018 17 4 1 1013 1 1016 0.0 2048
MS.gene53091.t1 MTR_6g477980 42.247 890 434 25 169 1013 261 1115 0.0 637
MS.gene53091.t1 MTR_6g477980 42.247 890 434 25 169 1013 255 1109 0.0 637
MS.gene53091.t1 MTR_1g102630 40.357 897 465 25 153 1013 116 978 0.0 612
MS.gene53091.t1 MTR_1g041345 40.223 895 467 25 152 1013 120 979 0.0 607
MS.gene53091.t1 MTR_4g056430 39.661 885 466 23 159 1013 125 971 0.0 602
MS.gene53091.t1 MTR_2g059590 39.201 926 493 25 122 1013 14 903 0.0 595
MS.gene53091.t1 MTR_2g028910 38.970 893 475 18 153 1012 173 1028 0.0 588
MS.gene53091.t1 MTR_4g056470 38.531 885 475 22 159 1013 163 1008 0.0 577
MS.gene53091.t1 MTR_4g083610 38.902 856 451 19 154 978 117 931 0.0 572
MS.gene53091.t1 MTR_4g113200 38.565 892 484 24 149 1013 22 876 0.0 570
MS.gene53091.t1 MTR_2g059790 42.454 603 305 13 433 1013 12 594 6.03e-151 461
MS.gene53091.t1 MTR_3g078647 31.853 901 522 29 159 1012 67 922 5.62e-124 400
MS.gene53091.t1 MTR_3g010650 31.579 931 526 34 135 1013 5 876 6.78e-122 394
MS.gene53091.t1 MTR_5g087870 29.494 929 550 29 139 1012 69 947 1.85e-114 376
MS.gene53091.t1 MTR_6g043180 29.957 928 540 29 138 1012 32 902 1.93e-112 369
MS.gene53091.t1 MTR_3g083300 31.475 915 531 28 151 1013 39 909 5.50e-112 368
MS.gene53091.t1 MTR_5g087890 29.859 921 539 33 138 1012 32 891 2.59e-111 366
MS.gene53091.t1 MTR_5g087870 29.053 919 545 30 139 1012 69 925 1.78e-108 359
MS.gene53091.t1 MTR_2g059590 34.443 691 405 21 122 792 14 676 1.36e-107 350
MS.gene53091.t1 MTR_1g038380 34.925 335 158 10 536 867 146 423 1.32e-40 156
MS.gene53091.t1 MTR_3g101730 41.860 172 68 4 740 911 33 172 8.83e-30 117
MS.gene53091.t1 MTR_6g037320 36.879 141 43 2 699 839 1 95 4.72e-19 84.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53091.t1 AT1G69440 70.905 873 216 7 149 1013 148 990 0.0 1285
MS.gene53091.t1 AT1G48410 39.135 1017 540 28 38 1013 70 1048 0.0 620
MS.gene53091.t1 AT1G48410 39.058 1019 540 29 38 1013 70 1050 0.0 616
MS.gene53091.t1 AT1G48410 39.058 1019 540 29 38 1013 70 1050 0.0 616
MS.gene53091.t1 AT5G43810 39.640 888 469 26 159 1013 135 988 0.0 593
MS.gene53091.t1 AT5G43810 39.640 888 469 26 159 1013 135 988 0.0 593
MS.gene53091.t1 AT5G43810 39.640 888 469 26 159 1013 135 988 0.0 593
MS.gene53091.t1 AT5G43810 39.640 888 469 26 159 1013 135 988 0.0 593
MS.gene53091.t1 AT2G27880 38.883 877 481 18 159 1013 154 997 0.0 588
MS.gene53091.t1 AT1G31280 36.686 845 474 19 153 976 163 967 8.85e-176 540
MS.gene53091.t1 AT1G31290 38.550 869 461 25 152 991 260 1084 8.39e-173 535
MS.gene53091.t1 AT1G31290 38.550 869 461 25 152 991 298 1122 2.23e-172 535
MS.gene53091.t1 AT1G31290 38.550 869 461 25 152 991 334 1158 6.02e-172 535
MS.gene53091.t1 AT5G21150 32.048 908 519 28 154 1013 39 896 8.25e-134 426
MS.gene53091.t1 AT2G27040 30.000 930 546 26 139 1013 45 924 3.67e-120 390
MS.gene53091.t1 AT2G27040 30.000 930 546 26 139 1013 45 924 3.67e-120 390
MS.gene53091.t1 AT5G21030 29.510 898 516 26 159 1013 31 854 1.12e-103 344
MS.gene53091.t1 AT2G32940 30.863 904 518 29 159 1013 33 878 4.88e-103 343
MS.gene53091.t1 AT5G21030 29.399 898 513 27 159 1013 31 850 2.81e-101 338

Find 194 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCCCATCAACTTCTTTAA+AGG 0.105814 5.4:-31507461 None:intergenic
TCTTAACAATGTCACAATTT+TGG 0.115828 5.4:+31509283 MS.gene53091:CDS
GAGGCTTATGAGTTACTTTA+AGG 0.128882 5.4:+31508392 MS.gene53091:CDS
AGAACTCAAGCTTATCATTT+TGG 0.173580 5.4:-31507765 None:intergenic
ACAGCTTCTTATTTGTATAA+TGG 0.263270 5.4:+31509349 MS.gene53091:CDS
TAGAGAAATTAAGCATAAAT+TGG 0.278567 5.4:+31507663 MS.gene53091:CDS
ACAACTACAAAAGTAATAAA+AGG 0.290192 5.4:-31509920 None:intergenic
GTTCACATCTTTGAGTTAAC+TGG 0.291747 5.4:-31509199 None:intergenic
GTTAACTGGTTTATGAATCT+TGG 0.293865 5.4:-31509185 None:intergenic
GAACTTAATTTGATGAGATT+TGG 0.299665 5.4:-31509452 None:intergenic
TTTCTATCTATGTAGTCATT+GGG 0.308144 5.4:+31510081 MS.gene53091:CDS
ATTGATCTTGAGAGCCAAAT+TGG 0.310485 5.4:-31509486 None:intergenic
AGGAGGCAACTGAAAATCTT+TGG 0.310887 5.4:+31508343 MS.gene53091:CDS
ATTTCTATCTATGTAGTCAT+TGG 0.316939 5.4:+31510080 MS.gene53091:CDS
GCAGGAGGAACTAATGAAAT+TGG 0.320784 5.4:-31510226 None:intergenic
GTCGATTTGCTAGTTGGAAT+TGG 0.327979 5.4:-31507799 None:intergenic
GTTTGCTTCTGAATTTCCTT+TGG 0.334202 5.4:-31507430 None:intergenic
TTCTCTAGCAACATCCTTAG+AGG 0.337003 5.4:-31507647 None:intergenic
TGCTGTTCACGTTTCTCATA+AGG 0.339409 5.4:-31507826 None:intergenic
AGATTCTTTCCATCTCTATC+AGG 0.345858 5.4:-31508369 None:intergenic
AATATCTTTGAAACTTGTTT+TGG 0.352913 5.4:-31506588 None:intergenic
CCATCACCAAGCTTTAGTTT+TGG 0.355041 5.4:-31509023 None:intergenic
ACAAATATATTTCAAGAATA+AGG 0.359133 5.4:+31509690 MS.gene53091:CDS
CTAGATGGCCATGTCTTTGA+AGG 0.360899 5.4:+31509098 MS.gene53091:CDS
GGCGCTTTGCCGGCATATGA+TGG 0.365628 5.4:+31507712 MS.gene53091:CDS
AGGTGGGCACTAATAAGTTT+TGG 0.365715 5.4:+31509134 MS.gene53091:CDS
TGCTCTCAATGTGGATTTCT+CGG 0.371590 5.4:+31508122 MS.gene53091:CDS
GTTCAGAGATACCGTGTCTA+TGG 0.373143 5.4:+31508312 MS.gene53091:CDS
CGGAAGAAAATGATTCGGTT+TGG 0.377730 5.4:-31509803 None:intergenic
GCATGTTCATCTAGGTTTCA+TGG 0.379673 5.4:+31509890 MS.gene53091:CDS
ATCTCTGAACAGTTTCTCTA+TGG 0.379706 5.4:-31508299 None:intergenic
CTTGAGAGATCAGAGTCCTT+AGG 0.384414 5.4:+31510289 MS.gene53091:CDS
GCAGGATTATCTACATGCTT+TGG 0.385806 5.4:+31507948 MS.gene53091:CDS
GTTGCAAGGAGACCAGATTC+TGG 0.386600 5.4:+31507520 MS.gene53091:CDS
ACTCAAAGATGTGAACAATT+AGG 0.388821 5.4:+31509206 MS.gene53091:CDS
CCTTCTTGTCCACCAGAATC+TGG 0.394128 5.4:-31507532 None:intergenic
AATTACTCCTCATCCCTCTA+AGG 0.396696 5.4:+31507633 MS.gene53091:CDS
TGCATGGAAGTTTGTGCAAA+TGG 0.396886 5.4:-31506684 None:intergenic
CCATCTGTCGCTGCTGTTGT+TGG 0.397087 5.4:+31509644 MS.gene53091:CDS
GAAGGTAAAGGAAGAAGAGC+AGG 0.402774 5.4:-31506613 None:intergenic
TAAAACAAGCATGTTCATCT+AGG 0.403433 5.4:+31509882 MS.gene53091:CDS
TGTAGAAACCAAGTTGAGTT+TGG 0.404992 5.4:-31506558 None:intergenic
GTCATTCTATTCGAGTTCAA+TGG 0.405912 5.4:+31508020 MS.gene53091:CDS
GGAAGAAAATGATTCGGTTT+GGG 0.408175 5.4:-31509802 None:intergenic
TCGATTTGCTAGTTGGAATT+GGG 0.409961 5.4:-31507798 None:intergenic
TCTCATGACGCCTTCGATGA+TGG 0.412144 5.4:-31508601 None:intergenic
AGATAGAAATCAAATTCCTT+TGG 0.415078 5.4:-31510067 None:intergenic
CCATCCTTTAAAGAAGTTGA+TGG 0.415148 5.4:+31507457 MS.gene53091:CDS
TCATTCTATTCGAGTTCAAT+GGG 0.415940 5.4:+31508021 MS.gene53091:CDS
CAACAATACCAAACTCAACT+TGG 0.417364 5.4:+31506550 MS.gene53091:CDS
ATAACTATGACATTCAATTC+AGG 0.422709 5.4:+31508421 MS.gene53091:CDS
TTGTGACATTGTTAAGAACT+TGG 0.423163 5.4:-31509277 None:intergenic
GAAAGATTGTATTCAGATGA+AGG 0.435201 5.4:-31506721 None:intergenic
ACTGTTGTTGTTGCTGTTGA+GGG 0.440124 5.4:-31506507 None:intergenic
TTAGGTAGAGGTGGTGTCTT+CGG 0.441067 5.4:-31510352 None:intergenic
CTGTTGTTGGTAGCATGAAC+TGG 0.441679 5.4:+31509657 MS.gene53091:CDS
TGACGAGCCGGTGATGTTCA+TGG 0.443980 5.4:+31509580 MS.gene53091:CDS
TACTGTTGTTGTTGCTGTTG+AGG 0.448198 5.4:-31506508 None:intergenic
ACTAAATACTTGAGCAAAGA+AGG 0.452270 5.4:+31507904 MS.gene53091:CDS
AAGAAGCTGTAGATTGTTTG+AGG 0.453692 5.4:-31509336 None:intergenic
AACAGTGCATCCACCAACTT+TGG 0.455056 5.4:-31509510 None:intergenic
CGCCAGTGGAACTTTCTAGA+TGG 0.456072 5.4:+31509083 MS.gene53091:CDS
GAGTTGTCTGCCTTTGAGAA+AGG 0.457326 5.4:-31508212 None:intergenic
TGTGAAGGACAGAAGTTCCT+TGG 0.458780 5.4:+31508510 MS.gene53091:CDS
CTCCTACTAATTTGCAAGCA+TGG 0.458921 5.4:-31508450 None:intergenic
GGTGACGTCGATTTGCTAGT+TGG 0.461530 5.4:-31507805 None:intergenic
GCACATTTAGCTGCATATAG+AGG 0.463633 5.4:+31510256 MS.gene53091:CDS
GAAATATATTTGTTTGCTGT+TGG 0.469845 5.4:-31509680 None:intergenic
TCTCAACTTTCACTTGATCC+TGG 0.472145 5.4:+31506742 MS.gene53091:CDS
CTTTGGTTTCCTGATAGAGA+TGG 0.476622 5.4:+31508360 MS.gene53091:CDS
TCGAGGCGTTTCTGCAAATA+CGG 0.479257 5.4:-31508177 None:intergenic
CAGATCACACAAAGCTCCAT+AGG 0.479814 5.4:-31508489 None:intergenic
GAAGATTGATCGGTGTCAGC+AGG 0.480032 5.4:-31509971 None:intergenic
TCCAACCATTGCACCGAGAT+CGG 0.481016 5.4:-31509741 None:intergenic
TACCCTCCAAAACTAAAGCT+TGG 0.483606 5.4:+31509017 MS.gene53091:CDS
CCATCAACTTCTTTAAAGGA+TGG 0.485273 5.4:-31507457 None:intergenic
TTTCGAAGATTGACGGGAAG+GGG 0.485680 5.4:+31507878 MS.gene53091:CDS
AACTAGTTTCGAAGATTGAC+GGG 0.490566 5.4:+31507872 MS.gene53091:CDS
AGAGAAATGACAAAGTTGAC+TGG 0.491533 5.4:+31508984 MS.gene53091:CDS
CTTGAATCGTCGAGAGGATG+AGG 0.492529 5.4:-31509620 None:intergenic
CAAGATGGGCTGTCAAAGAC+CGG 0.493342 5.4:+31508566 MS.gene53091:CDS
GGAGAAGTATTGAGTTTGCA+TGG 0.493972 5.4:-31506700 None:intergenic
CTCAGCTAACTTGTGAAGAA+AGG 0.494139 5.4:+31508232 MS.gene53091:CDS
GCATGGAAGTTTGTGCAAAT+GGG 0.494284 5.4:-31506683 None:intergenic
TGTAGATAATCCTGCGGAAG+CGG 0.495429 5.4:-31507940 None:intergenic
AGTTGTTGAAGTGAAGGTAA+AGG 0.498217 5.4:-31506625 None:intergenic
GAATAACTCCTATACTCTCA+TGG 0.500379 5.4:-31508155 None:intergenic
CAATATGAAAATATTCCACC+AGG 0.501529 5.4:+31510016 MS.gene53091:CDS
GAATAGAATGACCTTCCAAC+AGG 0.501556 5.4:-31508009 None:intergenic
GTTTCGAAGATTGACGGGAA+GGG 0.504847 5.4:+31507877 MS.gene53091:CDS
CGAAGGGATTCCGCTTCCGC+AGG 0.505337 5.4:+31507930 MS.gene53091:CDS
CTCAAGATCAATGCCAAAGT+TGG 0.507862 5.4:+31509497 MS.gene53091:CDS
GTGAAGGACAGAAGTTCCTT+GGG 0.508035 5.4:+31508511 MS.gene53091:CDS
ACTGCTTTCCATGAGAGTAT+AGG 0.509877 5.4:+31508147 MS.gene53091:CDS
TTGTCATTTCTCTTGAGACT+TGG 0.511045 5.4:-31508974 None:intergenic
TAAACCTTGTTATCTCCCTA+TGG 0.511898 5.4:+31508473 MS.gene53091:CDS
CATCCTTTAAAGAAGTTGAT+GGG 0.514420 5.4:+31507458 MS.gene53091:CDS
TGATAATGATTTAGCACCAA+AGG 0.516884 5.4:+31507414 MS.gene53091:intron
AAGATCAATGCCAAAGTTGG+TGG 0.519312 5.4:+31509500 MS.gene53091:CDS
AGAGCAAGTCCTTGTTGTGT+TGG 0.520077 5.4:-31508105 None:intergenic
GCCGATCTCGGTGCAATGGT+TGG 0.524340 5.4:+31509740 MS.gene53091:CDS
GGCCATGCTTGCAAATTAGT+AGG 0.524814 5.4:+31508448 MS.gene53091:CDS
ATGTAGTCATTGGGGTGTGA+AGG 0.526206 5.4:+31510090 MS.gene53091:CDS
GCGCTTTGCCGGCATATGAT+GGG 0.527480 5.4:+31507713 MS.gene53091:CDS
GATATCGGTGGAGGAGCTGT+TGG 0.528041 5.4:+31508054 MS.gene53091:CDS
CTTGCAACAATTACTTCATG+TGG 0.531075 5.4:-31507505 None:intergenic
ACAGAATCAACCACAGTCCC+TGG 0.531566 5.4:-31510034 None:intergenic
TGAGGGAAAGATGGAATCAA+AGG 0.534413 5.4:-31506649 None:intergenic
GACGAGCCGGTGATGTTCAT+GGG 0.534686 5.4:+31509581 MS.gene53091:CDS
CAAGAAATTATTGCCGATCT+CGG 0.535339 5.4:+31509728 MS.gene53091:CDS
ATGGGGAGAAGCAAAGATAT+CGG 0.538077 5.4:+31508039 MS.gene53091:CDS
GAGGAAATGTTGGTCCTACC+AGG 0.540340 5.4:+31508622 MS.gene53091:CDS
AAAGGTAGTTGTTGAAGTGA+AGG 0.542969 5.4:-31506631 None:intergenic
GCTAAATGTGCATAATATGC+AGG 0.543695 5.4:-31510244 None:intergenic
GGAGGAGCTGTTGGATTGAG+AGG 0.544646 5.4:+31508063 MS.gene53091:CDS
GATTGTTTGAGGCAATACTC+TGG 0.544839 5.4:-31509325 None:intergenic
GCTAACTTGTGAAGAAAGGA+AGG 0.550241 5.4:+31508236 MS.gene53091:CDS
TTGAAGGAACTACAATTGAA+AGG 0.550534 5.4:+31509114 MS.gene53091:CDS
GAACGAAAAGCCATCATCGA+AGG 0.552388 5.4:+31508591 MS.gene53091:CDS
CACAAAACATGGTAATGAGT+TGG 0.552639 5.4:-31510124 None:intergenic
CCAGATTCTGGTGGACAAGA+AGG 0.553420 5.4:+31507532 MS.gene53091:CDS
ACTGAGAAATGTATACCTGT+TGG 0.554582 5.4:+31507994 MS.gene53091:CDS
ATGACATTCAATTCAGGAAG+TGG 0.554927 5.4:+31508427 MS.gene53091:CDS
CGTCTCGGAAGAAAATGATT+CGG 0.555994 5.4:-31509808 None:intergenic
CTAAGGACTCTGATCTCTCA+AGG 0.557408 5.4:-31510288 None:intergenic
TATTGCCGATCTCGGTGCAA+TGG 0.560805 5.4:+31509736 MS.gene53091:CDS
GATTTGGATACAAGCAGCAC+TGG 0.562263 5.4:-31509436 None:intergenic
AAACTAGTTTCGAAGATTGA+CGG 0.563664 5.4:+31507871 MS.gene53091:CDS
TCTCTAGCAACATCCTTAGA+GGG 0.563703 5.4:-31507646 None:intergenic
CCAAAACTAAAGCTTGGTGA+TGG 0.566399 5.4:+31509023 MS.gene53091:CDS
TCACTAAGTTTAGGTAGAGG+TGG 0.567563 5.4:-31510361 None:intergenic
TGTAGTCATTGGGGTGTGAA+GGG 0.568049 5.4:+31510091 MS.gene53091:CDS
TTCTGTGATTACTCATCCAA+AGG 0.568582 5.4:+31510051 MS.gene53091:CDS
CACTGGCGATCGTGACGTGA+AGG 0.568663 5.4:-31509068 None:intergenic
TTCTATCTATGTAGTCATTG+GGG 0.573801 5.4:+31510082 MS.gene53091:CDS
GAGAAATGACAAAGTTGACT+GGG 0.575964 5.4:+31508985 MS.gene53091:CDS
CCAACAACAGCAGCGACAGA+TGG 0.580449 5.4:-31509644 None:intergenic
AAGGTAAAGGAAGAAGAGCA+GGG 0.582117 5.4:-31506612 None:intergenic
AGCACTTCTGAACAAACCTA+AGG 0.582641 5.4:-31510305 None:intergenic
ATTTGCTAGTTGGAATTGGG+AGG 0.582837 5.4:-31507795 None:intergenic
GCAACATCCTTAGAGGGATG+AGG 0.583299 5.4:-31507640 None:intergenic
AAGGAACTACAATTGAAAGG+TGG 0.584900 5.4:+31509117 MS.gene53091:CDS
ACTTGGATTGATTCAAACTG+TGG 0.587733 5.4:-31509260 None:intergenic
AGTTTCGAAGATTGACGGGA+AGG 0.591637 5.4:+31507876 MS.gene53091:CDS
AGGAACTACAATTGAAAGGT+GGG 0.592411 5.4:+31509118 MS.gene53091:CDS
AAACTAAAGCTTGGTGATGG+AGG 0.594557 5.4:+31509026 MS.gene53091:CDS
GGCGTCATGAGAGGAAATGT+TGG 0.595199 5.4:+31508612 MS.gene53091:CDS
TTGTAGTTCCTTCAAAGACA+TGG 0.597249 5.4:-31509106 None:intergenic
AGAAGCAAAGATATCGGTGG+AGG 0.598026 5.4:+31508045 MS.gene53091:CDS
AGATCACACAAAGCTCCATA+GGG 0.598624 5.4:-31508488 None:intergenic
GGCCATCTAGAAAGTTCCAC+TGG 0.600669 5.4:-31509085 None:intergenic
ATCATCGAAGGCGTCATGAG+AGG 0.605292 5.4:+31508603 MS.gene53091:CDS
TTCTTAGAGACCTTTCTCAA+AGG 0.606629 5.4:+31508202 MS.gene53091:CDS
CACATGAAGTAATTGTTGCA+AGG 0.609332 5.4:+31507506 MS.gene53091:CDS
ATAGAGATGGAAAGAATCTG+AGG 0.612806 5.4:+31508373 MS.gene53091:CDS
TACAACACCGACACTTGTCT+CGG 0.616242 5.4:-31509411 None:intergenic
TCAGCTCCCATGAACATCAC+CGG 0.616360 5.4:-31509587 None:intergenic
TGAGCAAAGAAGGTGACGAA+GGG 0.621730 5.4:+31507914 MS.gene53091:CDS
GAAACTGTCGGATGATCAGA+CGG 0.621779 5.4:+31508533 MS.gene53091:CDS
AAATATTCCACCAGGGACTG+TGG 0.623325 5.4:+31510024 MS.gene53091:CDS
AAAAGACGCGGTAATTGAGA+AGG 0.623652 5.4:-31509551 None:intergenic
TTGAGCAAAGAAGGTGACGA+AGG 0.624886 5.4:+31507913 MS.gene53091:CDS
CTTCACGTCACGATCGCCAG+TGG 0.626226 5.4:+31509069 MS.gene53091:CDS
AAGATGGGCTGTCAAAGACC+GGG 0.633687 5.4:+31508567 MS.gene53091:CDS
GAGTTTCGCTAACACCGTCT+CGG 0.634375 5.4:-31509823 None:intergenic
GAAGTTCCTTGGGAAACTGT+CGG 0.634410 5.4:+31508521 MS.gene53091:CDS
CGAATTGCCGAGACAAGTGT+CGG 0.636497 5.4:+31509404 MS.gene53091:CDS
ACAGGTATACATTTCTCAGT+CGG 0.639287 5.4:-31507991 None:intergenic
GAGCTTTGTGTGATCTGTGA+AGG 0.639487 5.4:+31508495 MS.gene53091:CDS
AGAAATGTATACCTGTTGGA+AGG 0.640017 5.4:+31507998 MS.gene53091:CDS
AGCTCCATAGGGAGATAACA+AGG 0.643271 5.4:-31508477 None:intergenic
ACAAGGACTTGCTCTCAATG+TGG 0.644006 5.4:+31508113 MS.gene53091:CDS
AGTCTTAGACCAACACAACA+AGG 0.644925 5.4:+31508096 MS.gene53091:CDS
GTTGTGCGTCGAAGATTGAT+CGG 0.651284 5.4:-31509981 None:intergenic
GGAAGAAGCTTATTTCAACA+AGG 0.658895 5.4:+31507479 MS.gene53091:CDS
CTTTGCCGGCATATGATGGG+AGG 0.659001 5.4:+31507716 MS.gene53091:CDS
TGTCTATGGCTTAACTGAGG+AGG 0.661469 5.4:+31508326 MS.gene53091:CDS
AAATGTGCATAATATGCAGG+AGG 0.662875 5.4:-31510241 None:intergenic
GGGAGAAGCAAAGATATCGG+TGG 0.666198 5.4:+31508042 MS.gene53091:CDS
GCAAGGAGACCAGATTCTGG+TGG 0.670025 5.4:+31507523 MS.gene53091:CDS
AAAGGTCTCTAAGAAACTCG+AGG 0.671596 5.4:-31508194 None:intergenic
AATATGAAAATATTCCACCA+GGG 0.673069 5.4:+31510017 MS.gene53091:CDS
GATCATCCGACAGTTTCCCA+AGG 0.673171 5.4:-31508527 None:intergenic
GCATTGCAGTTATACCTGGT+AGG 0.680391 5.4:-31508636 None:intergenic
GAATCAACCACAGTCCCTGG+TGG 0.680648 5.4:-31510031 None:intergenic
TTGGCAAGGAGAGAAATCAC+AGG 0.686931 5.4:-31507556 None:intergenic
GATGGACTTGAATCGTCGAG+AGG 0.693236 5.4:-31509626 None:intergenic
GGGGTGTGAAGGGAACAAGT+AGG 0.696897 5.4:+31510101 MS.gene53091:CDS
CATTCTATTCGAGTTCAATG+GGG 0.697995 5.4:+31508022 MS.gene53091:CDS
TCGTCAATGTTGAAAAGACG+CGG 0.703990 5.4:-31509563 None:intergenic
GAATAAGGTCTCAAACACAC+AGG 0.710521 5.4:+31509705 MS.gene53091:CDS
CCTCAGTTAAGCCATAGACA+CGG 0.718867 5.4:-31508323 None:intergenic
AAAGCATGTAGATAATCCTG+CGG 0.726216 5.4:-31507946 None:intergenic
CCGTGTCTATGGCTTAACTG+AGG 0.727424 5.4:+31508323 MS.gene53091:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCATTATCATAAATAAGA+TGG - chr5.4:31507405-31507424 None:intergenic 15.0%
!! ACAAATATATTTCAAGAATA+AGG + chr5.4:31509690-31509709 MS.gene53091:CDS 15.0%
!! CAACTATATTAAAAAAAACA+AGG - chr5.4:31506827-31506846 None:intergenic 15.0%
!!! TTAAATTTACTTTGACTTAT+TGG + chr5.4:31506898-31506917 MS.gene53091:intron 15.0%
!! ACAACTACAAAAGTAATAAA+AGG - chr5.4:31509923-31509942 None:intergenic 20.0%
!! TAGAGAAATTAAGCATAAAT+TGG + chr5.4:31507663-31507682 MS.gene53091:CDS 20.0%
!!! AATATCTTTGAAACTTGTTT+TGG - chr5.4:31506591-31506610 None:intergenic 20.0%
!!! ACTTTTTAAACATTGCTTTT+GGG + chr5.4:31506969-31506988 MS.gene53091:intron 20.0%
!!! ATTGTGCATTTTTTTAACAT+TGG + chr5.4:31507020-31507039 MS.gene53091:intron 20.0%
!!! ATTTGTGTTTTACTTTTGTT+AGG + chr5.4:31510194-31510213 MS.gene53091:CDS 20.0%
!!! TACTTTTTAAACATTGCTTT+TGG + chr5.4:31506968-31506987 MS.gene53091:intron 20.0%
!!! TTATCATTTTGGAATTCAAT+TGG - chr5.4:31507757-31507776 None:intergenic 20.0%
! AATATGAAAATATTCCACCA+GGG + chr5.4:31510017-31510036 MS.gene53091:CDS 25.0%
! AATCAAACTTCACCAAAAAA+TGG - chr5.4:31507582-31507601 None:intergenic 25.0%
! AGATAGAAATCAAATTCCTT+TGG - chr5.4:31510070-31510089 None:intergenic 25.0%
! ATAACTATGACATTCAATTC+AGG + chr5.4:31508421-31508440 MS.gene53091:CDS 25.0%
! ATTTCTATCTATGTAGTCAT+TGG + chr5.4:31510080-31510099 MS.gene53091:CDS 25.0%
! CAAAATCTCACAAACAAAAA+TGG + chr5.4:31506445-31506464 MS.gene53091:CDS 25.0%
! CTTAAAAAACCAAACAAACA+AGG - chr5.4:31506870-31506889 None:intergenic 25.0%
! CTTATGTGAAGAATGTTATT+AGG - chr5.4:31507308-31507327 None:intergenic 25.0%
! TAATGGAGAAAAAACACAAA+GGG + chr5.4:31509366-31509385 MS.gene53091:CDS 25.0%
! TGAAAATGTAAGTTCTTGTT+TGG + chr5.4:31507060-31507079 MS.gene53091:intron 25.0%
! TTTCTATCTATGTAGTCATT+GGG + chr5.4:31510081-31510100 MS.gene53091:CDS 25.0%
!! AAAATGTAAGTTCTTGTTTG+GGG + chr5.4:31507062-31507081 MS.gene53091:intron 25.0%
!! ACAGCTTCTTATTTGTATAA+TGG + chr5.4:31509349-31509368 MS.gene53091:CDS 25.0%
!! ATAATGGAGAAAAAACACAA+AGG + chr5.4:31509365-31509384 MS.gene53091:CDS 25.0%
!! CAAATTAAGTTCACAGTTTT+TGG + chr5.4:31509463-31509482 MS.gene53091:CDS 25.0%
!! GAAAATGTAAGTTCTTGTTT+GGG + chr5.4:31507061-31507080 MS.gene53091:intron 25.0%
!! GAACTTAATTTGATGAGATT+TGG - chr5.4:31509455-31509474 None:intergenic 25.0%
!! TAACAATGCTGAGATTTTAT+CGG + chr5.4:31507690-31507709 MS.gene53091:CDS 25.0%
!! TCTTAACAATGTCACAATTT+TGG + chr5.4:31509283-31509302 MS.gene53091:CDS 25.0%
!! TGAAGATTTTTATCAAGAAG+TGG + chr5.4:31509772-31509791 MS.gene53091:CDS 25.0%
!! TTGATGTTTTCACTAAGTTT+AGG - chr5.4:31510373-31510392 None:intergenic 25.0%
!!! ATTTTGGTTAGTGTTTTTGT+TGG - chr5.4:31506431-31506450 None:intergenic 25.0%
!!! CATTTTTGTTTGTGAGATTT+TGG - chr5.4:31506447-31506466 None:intergenic 25.0%
!!! CTTTTTAAACATTGCTTTTG+GGG + chr5.4:31506970-31506989 MS.gene53091:intron 25.0%
!!! GAAATATATTTGTTTGCTGT+TGG - chr5.4:31509683-31509702 None:intergenic 25.0%
AAACTTCACCAAAAAATGGT+TGG - chr5.4:31507578-31507597 None:intergenic 30.0%
ACTAAATACTTGAGCAAAGA+AGG + chr5.4:31507904-31507923 MS.gene53091:CDS 30.0%
ACTCAAAGATGTGAACAATT+AGG + chr5.4:31509206-31509225 MS.gene53091:CDS 30.0%
ATGAAAAGAACAGTGAAGAA+TGG - chr5.4:31506778-31506797 None:intergenic 30.0%
CAATATGAAAATATTCCACC+AGG + chr5.4:31510016-31510035 MS.gene53091:CDS 30.0%
CCACGTAATCACTTAATAAA+CGG - chr5.4:31508819-31508838 None:intergenic 30.0%
GAAAGATTGTATTCAGATGA+AGG - chr5.4:31506724-31506743 None:intergenic 30.0%
TAAAACAAGCATGTTCATCT+AGG + chr5.4:31509882-31509901 MS.gene53091:CDS 30.0%
TCACTTAATAAACGGTTTCA+CGG - chr5.4:31508811-31508830 None:intergenic 30.0%
TTATTAAGTGATTACGTGGA+CGG + chr5.4:31508820-31508839 MS.gene53091:intron 30.0%
TTCTATCTATGTAGTCATTG+GGG + chr5.4:31510082-31510101 MS.gene53091:CDS 30.0%
TTGAAGGAACTACAATTGAA+AGG + chr5.4:31509114-31509133 MS.gene53091:CDS 30.0%
TTGTGACATTGTTAAGAACT+TGG - chr5.4:31509280-31509299 None:intergenic 30.0%
! AAAAGTAATCCTTTTGACAG+TGG + chr5.4:31508927-31508946 MS.gene53091:intron 30.0%
! AAACTAGTTTCGAAGATTGA+CGG + chr5.4:31507871-31507890 MS.gene53091:CDS 30.0%
! AAATGTAAGTTCTTGTTTGG+GGG + chr5.4:31507063-31507082 MS.gene53091:intron 30.0%
! AACAATGCTGAGATTTTATC+GGG + chr5.4:31507691-31507710 MS.gene53091:CDS 30.0%
! AACTCATTACCATGTTTTGT+GGG + chr5.4:31510126-31510145 MS.gene53091:CDS 30.0%
! AGAACTCAAGCTTATCATTT+TGG - chr5.4:31507768-31507787 None:intergenic 30.0%
! CATCCTTTAAAGAAGTTGAT+GGG + chr5.4:31507458-31507477 MS.gene53091:CDS 30.0%
! CTTTTGTAGTTGTGCAAAAA+AGG + chr5.4:31509930-31509949 MS.gene53091:CDS 30.0%
! GTGAACAGCATAAACTTTTT+CGG + chr5.4:31507839-31507858 MS.gene53091:CDS 30.0%
! GTTAACTGGTTTATGAATCT+TGG - chr5.4:31509188-31509207 None:intergenic 30.0%
! TATTTTCATCCCACAAAACA+TGG - chr5.4:31510138-31510157 None:intergenic 30.0%
! TCATTCTATTCGAGTTCAAT+GGG + chr5.4:31508021-31508040 MS.gene53091:CDS 30.0%
! TGATAATGATTTAGCACCAA+AGG + chr5.4:31507414-31507433 MS.gene53091:intron 30.0%
! TTTTCACTAAGTTTAGGTAG+AGG - chr5.4:31510367-31510386 None:intergenic 30.0%
!! TTCTTTAAGCCTTGTTTGTT+TGG + chr5.4:31506858-31506877 MS.gene53091:intron 30.0%
!!! AAATGGGTTTTGAGATTTTG+AGG - chr5.4:31506670-31506689 None:intergenic 30.0%
!!! AATGGGTTTTGAGATTTTGA+GGG - chr5.4:31506669-31506688 None:intergenic 30.0%
AAACAAGGAATGCAAGCAAA+AGG - chr5.4:31506812-31506831 None:intergenic 35.0%
AAAGCATGTAGATAATCCTG+CGG - chr5.4:31507949-31507968 None:intergenic 35.0%
AAAGGTAGTTGTTGAAGTGA+AGG - chr5.4:31506634-31506653 None:intergenic 35.0%
AAATGTGCATAATATGCAGG+AGG - chr5.4:31510244-31510263 None:intergenic 35.0%
AACAAGGAATGCAAGCAAAA+GGG - chr5.4:31506811-31506830 None:intergenic 35.0%
AAGAAGCTGTAGATTGTTTG+AGG - chr5.4:31509339-31509358 None:intergenic 35.0%
AAGGAACTACAATTGAAAGG+TGG + chr5.4:31509117-31509136 MS.gene53091:CDS 35.0%
AAGTGAAACGATCACTTGTT+TGG + chr5.4:31507187-31507206 MS.gene53091:intron 35.0%
ACAGGTATACATTTCTCAGT+CGG - chr5.4:31507994-31508013 None:intergenic 35.0%
ACGGCAAAAATACTCAAGAT+GGG + chr5.4:31508552-31508571 MS.gene53091:CDS 35.0%
ACTGAGAAATGTATACCTGT+TGG + chr5.4:31507994-31508013 MS.gene53091:CDS 35.0%
AGAAATGTATACCTGTTGGA+AGG + chr5.4:31507998-31508017 MS.gene53091:CDS 35.0%
AGAGAAATGACAAAGTTGAC+TGG + chr5.4:31508984-31509003 MS.gene53091:CDS 35.0%
AGATTCTTTCCATCTCTATC+AGG - chr5.4:31508372-31508391 None:intergenic 35.0%
AGGAACTACAATTGAAAGGT+GGG + chr5.4:31509118-31509137 MS.gene53091:CDS 35.0%
AGTTGTTGAAGTGAAGGTAA+AGG - chr5.4:31506628-31506647 None:intergenic 35.0%
ATAGAGATGGAAAGAATCTG+AGG + chr5.4:31508373-31508392 MS.gene53091:CDS 35.0%
ATCTCTGAACAGTTTCTCTA+TGG - chr5.4:31508302-31508321 None:intergenic 35.0%
ATGACATTCAATTCAGGAAG+TGG + chr5.4:31508427-31508446 MS.gene53091:CDS 35.0%
ATTGATCTTGAGAGCCAAAT+TGG - chr5.4:31509489-31509508 None:intergenic 35.0%
CAACAATACCAAACTCAACT+TGG + chr5.4:31506550-31506569 MS.gene53091:CDS 35.0%
CAAGAAATTATTGCCGATCT+CGG + chr5.4:31509728-31509747 MS.gene53091:CDS 35.0%
CACATGAAGTAATTGTTGCA+AGG + chr5.4:31507506-31507525 MS.gene53091:CDS 35.0%
CCGTTTATTAAGTGATTACG+TGG + chr5.4:31508816-31508835 MS.gene53091:intron 35.0%
CTTCCCATCAACTTCTTTAA+AGG - chr5.4:31507464-31507483 None:intergenic 35.0%
CTTGCAACAATTACTTCATG+TGG - chr5.4:31507508-31507527 None:intergenic 35.0%
GAATAACTCCTATACTCTCA+TGG - chr5.4:31508158-31508177 None:intergenic 35.0%
GAGAAATGACAAAGTTGACT+GGG + chr5.4:31508985-31509004 MS.gene53091:CDS 35.0%
GCTAAATGTGCATAATATGC+AGG - chr5.4:31510247-31510266 None:intergenic 35.0%
GGAAGAAGCTTATTTCAACA+AGG + chr5.4:31507479-31507498 MS.gene53091:CDS 35.0%
GTTTGCTTCTGAATTTCCTT+TGG - chr5.4:31507433-31507452 None:intergenic 35.0%
TTCTGTGATTACTCATCCAA+AGG + chr5.4:31510051-31510070 MS.gene53091:CDS 35.0%
TTCTTAGAGACCTTTCTCAA+AGG + chr5.4:31508202-31508221 MS.gene53091:CDS 35.0%
TTGTAGTTCCTTCAAAGACA+TGG - chr5.4:31509109-31509128 None:intergenic 35.0%
TTGTTTGGGGGAATACTTTA+AGG + chr5.4:31507075-31507094 MS.gene53091:intron 35.0%
! AACTAGTTTCGAAGATTGAC+GGG + chr5.4:31507872-31507891 MS.gene53091:CDS 35.0%
! ACTTGGATTGATTCAAACTG+TGG - chr5.4:31509263-31509282 None:intergenic 35.0%
! CAACTCATTACCATGTTTTG+TGG + chr5.4:31510125-31510144 MS.gene53091:CDS 35.0%
! CACAAAACATGGTAATGAGT+TGG - chr5.4:31510127-31510146 None:intergenic 35.0%
! CATTCTATTCGAGTTCAATG+GGG + chr5.4:31508022-31508041 MS.gene53091:CDS 35.0%
! CCATCAACTTCTTTAAAGGA+TGG - chr5.4:31507460-31507479 None:intergenic 35.0%
! CCATCCTTTAAAGAAGTTGA+TGG + chr5.4:31507457-31507476 MS.gene53091:CDS 35.0%
! GAGGCTTATGAGTTACTTTA+AGG + chr5.4:31508392-31508411 MS.gene53091:CDS 35.0%
! GGAGTTTGAATTCATTTTCC+TGG - chr5.4:31508956-31508975 None:intergenic 35.0%
! GTCATTCTATTCGAGTTCAA+TGG + chr5.4:31508020-31508039 MS.gene53091:CDS 35.0%
! GTTCACATCTTTGAGTTAAC+TGG - chr5.4:31509202-31509221 None:intergenic 35.0%
! GTTTTACAAAGTTCTGCAAG+AGG + chr5.4:31509847-31509866 MS.gene53091:CDS 35.0%
! TAAACCTTGTTATCTCCCTA+TGG + chr5.4:31508473-31508492 MS.gene53091:CDS 35.0%
! TGTAGAAACCAAGTTGAGTT+TGG - chr5.4:31506561-31506580 None:intergenic 35.0%
! TTGTCATTTCTCTTGAGACT+TGG - chr5.4:31508977-31508996 None:intergenic 35.0%
!! ATGCTTTGGATGTAGTTTTG+AGG + chr5.4:31507962-31507981 MS.gene53091:CDS 35.0%
!! GGAAGAAAATGATTCGGTTT+GGG - chr5.4:31509805-31509824 None:intergenic 35.0%
!! TCGATTTGCTAGTTGGAATT+GGG - chr5.4:31507801-31507820 None:intergenic 35.0%
!! TTGAGATTTTGAGGGAAAGA+TGG - chr5.4:31506661-31506680 None:intergenic 35.0%
!!! TGCTTTGGATGTAGTTTTGA+GGG + chr5.4:31507963-31507982 MS.gene53091:CDS 35.0%
!!! TTCACAGTTTTTGGCCAATT+TGG + chr5.4:31509472-31509491 MS.gene53091:CDS 35.0%
AAAAGACGCGGTAATTGAGA+AGG - chr5.4:31509554-31509573 None:intergenic 40.0%
AAGAATGGTGCTTACTTACC+AGG - chr5.4:31506763-31506782 None:intergenic 40.0%
AAGATCAATGCCAAAGTTGG+TGG + chr5.4:31509500-31509519 MS.gene53091:CDS 40.0%
AAGGTAAAGGAAGAAGAGCA+GGG - chr5.4:31506615-31506634 None:intergenic 40.0%
AATTACTCCTCATCCCTCTA+AGG + chr5.4:31507633-31507652 MS.gene53091:CDS 40.0%
AGATCACACAAAGCTCCATA+GGG - chr5.4:31508491-31508510 None:intergenic 40.0%
AGCACTTCTGAACAAACCTA+AGG - chr5.4:31510308-31510327 None:intergenic 40.0%
AGGAGGCAACTGAAAATCTT+TGG + chr5.4:31508343-31508362 MS.gene53091:CDS 40.0%
AGTCTTAGACCAACACAACA+AGG + chr5.4:31508096-31508115 MS.gene53091:CDS 40.0%
ATGGGGAGAAGCAAAGATAT+CGG + chr5.4:31508039-31508058 MS.gene53091:CDS 40.0%
CCTCATAAAAACCAGAACAC+TGG + chr5.4:31508757-31508776 MS.gene53091:intron 40.0%
CGTCTCGGAAGAAAATGATT+CGG - chr5.4:31509811-31509830 None:intergenic 40.0%
CTCAAGATCAATGCCAAAGT+TGG + chr5.4:31509497-31509516 MS.gene53091:CDS 40.0%
GAATAAGGTCTCAAACACAC+AGG + chr5.4:31509705-31509724 MS.gene53091:CDS 40.0%
GAATAGAATGACCTTCCAAC+AGG - chr5.4:31508012-31508031 None:intergenic 40.0%
GACGGCAAAAATACTCAAGA+TGG + chr5.4:31508551-31508570 MS.gene53091:CDS 40.0%
GCACATTTAGCTGCATATAG+AGG + chr5.4:31510256-31510275 MS.gene53091:CDS 40.0%
GCAGGAGGAACTAATGAAAT+TGG - chr5.4:31510229-31510248 None:intergenic 40.0%
GCAGGATTATCTACATGCTT+TGG + chr5.4:31507948-31507967 MS.gene53091:CDS 40.0%
GCATGGAAGTTTGTGCAAAT+GGG - chr5.4:31506686-31506705 None:intergenic 40.0%
GCATGTTCATCTAGGTTTCA+TGG + chr5.4:31509890-31509909 MS.gene53091:CDS 40.0%
GTTAAGATACTCCAGTGTTC+TGG - chr5.4:31508771-31508790 None:intergenic 40.0%
TACCCTCCAAAACTAAAGCT+TGG + chr5.4:31509017-31509036 MS.gene53091:CDS 40.0%
TAGTGCATTGCAGTTATACC+TGG - chr5.4:31508643-31508662 None:intergenic 40.0%
TCACCAAAAAATGGTTGGCA+AGG - chr5.4:31507573-31507592 None:intergenic 40.0%
TCACTAAGTTTAGGTAGAGG+TGG - chr5.4:31510364-31510383 None:intergenic 40.0%
TCGTCAATGTTGAAAAGACG+CGG - chr5.4:31509566-31509585 None:intergenic 40.0%
TCTCAACTTTCACTTGATCC+TGG + chr5.4:31506742-31506761 MS.gene53091:CDS 40.0%
TCTCTAGCAACATCCTTAGA+GGG - chr5.4:31507649-31507668 None:intergenic 40.0%
TGAGGGAAAGATGGAATCAA+AGG - chr5.4:31506652-31506671 None:intergenic 40.0%
TGCATGGAAGTTTGTGCAAA+TGG - chr5.4:31506687-31506706 None:intergenic 40.0%
TGCTCTCAATGTGGATTTCT+CGG + chr5.4:31508122-31508141 MS.gene53091:CDS 40.0%
TGCTGTTCACGTTTCTCATA+AGG - chr5.4:31507829-31507848 None:intergenic 40.0%
TTCTCTAGCAACATCCTTAG+AGG - chr5.4:31507650-31507669 None:intergenic 40.0%
! AAAGGTCTCTAAGAAACTCG+AGG - chr5.4:31508197-31508216 None:intergenic 40.0%
! ACTGCTTTCCATGAGAGTAT+AGG + chr5.4:31508147-31508166 MS.gene53091:CDS 40.0%
! ACTGTTGTTGTTGCTGTTGA+GGG - chr5.4:31506510-31506529 None:intergenic 40.0%
! AGGTGGGCACTAATAAGTTT+TGG + chr5.4:31509134-31509153 MS.gene53091:CDS 40.0%
! ATTTGCTAGTTGGAATTGGG+AGG - chr5.4:31507798-31507817 None:intergenic 40.0%
! CCAAAACTAAAGCTTGGTGA+TGG + chr5.4:31509023-31509042 MS.gene53091:CDS 40.0%
! CGAATCATTTTCTTCCGAGA+CGG + chr5.4:31509809-31509828 MS.gene53091:CDS 40.0%
! CTCAGCTAACTTGTGAAGAA+AGG + chr5.4:31508232-31508251 MS.gene53091:CDS 40.0%
! CTCCTACTAATTTGCAAGCA+TGG - chr5.4:31508453-31508472 None:intergenic 40.0%
! CTTTGGTTTCCTGATAGAGA+TGG + chr5.4:31508360-31508379 MS.gene53091:CDS 40.0%
! GATTGTTTGAGGCAATACTC+TGG - chr5.4:31509328-31509347 None:intergenic 40.0%
! GCTAACTTGTGAAGAAAGGA+AGG + chr5.4:31508236-31508255 MS.gene53091:CDS 40.0%
! GGAATATGAGACTTTTGCTC+AGG - chr5.4:31509167-31509186 None:intergenic 40.0%
! TACTGTTGTTGTTGCTGTTG+AGG - chr5.4:31506511-31506530 None:intergenic 40.0%
! TCTTTTCAACATTGACGAGC+CGG + chr5.4:31509568-31509587 MS.gene53091:CDS 40.0%
! TTTTTCCTCCCATCATATGC+CGG - chr5.4:31507724-31507743 None:intergenic 40.0%
!! AAACTAAAGCTTGGTGATGG+AGG + chr5.4:31509026-31509045 MS.gene53091:CDS 40.0%
!! CCATCACCAAGCTTTAGTTT+TGG - chr5.4:31509026-31509045 None:intergenic 40.0%
!! CGGAAGAAAATGATTCGGTT+TGG - chr5.4:31509806-31509825 None:intergenic 40.0%
!! GGAGAAGTATTGAGTTTGCA+TGG - chr5.4:31506703-31506722 None:intergenic 40.0%
!! GTCGATTTGCTAGTTGGAAT+TGG - chr5.4:31507802-31507821 None:intergenic 40.0%
!! TGCAAAAAAGGCATCACACA+AGG + chr5.4:31509942-31509961 MS.gene53091:CDS 40.0%
!! TGGGCACTAATAAGTTTTGG+AGG + chr5.4:31509137-31509156 MS.gene53091:CDS 40.0%
!!! CACCAAGCTTTAGTTTTGGA+GGG - chr5.4:31509022-31509041 None:intergenic 40.0%
!!! CCAGTGTTCTGGTTTTTATG+AGG - chr5.4:31508760-31508779 None:intergenic 40.0%
!!! TCACCAAGCTTTAGTTTTGG+AGG - chr5.4:31509023-31509042 None:intergenic 40.0%
!!! TCTCCTTGCCAACCATTTTT+TGG + chr5.4:31507567-31507586 MS.gene53091:CDS 40.0%
AAATATTCCACCAGGGACTG+TGG + chr5.4:31510024-31510043 MS.gene53091:CDS 45.0%
AACAGTGCATCCACCAACTT+TGG - chr5.4:31509513-31509532 None:intergenic 45.0%
ACGGTTTCACGGTTTGTGAA+TGG - chr5.4:31508800-31508819 None:intergenic 45.0%
AGAAGCAAAGATATCGGTGG+AGG + chr5.4:31508045-31508064 MS.gene53091:CDS 45.0%
AGCTCCATAGGGAGATAACA+AGG - chr5.4:31508480-31508499 None:intergenic 45.0%
AGTTTCGAAGATTGACGGGA+AGG + chr5.4:31507876-31507895 MS.gene53091:CDS 45.0%
ATGTAGTCATTGGGGTGTGA+AGG + chr5.4:31510090-31510109 MS.gene53091:CDS 45.0%
CACGGTTTGTGAATGGATGT+TGG - chr5.4:31508793-31508812 None:intergenic 45.0%
CAGATCACACAAAGCTCCAT+AGG - chr5.4:31508492-31508511 None:intergenic 45.0%
CCTCAGTTAAGCCATAGACA+CGG - chr5.4:31508326-31508345 None:intergenic 45.0%
CTAAGGACTCTGATCTCTCA+AGG - chr5.4:31510291-31510310 None:intergenic 45.0%
CTAGATGGCCATGTCTTTGA+AGG + chr5.4:31509098-31509117 MS.gene53091:CDS 45.0%
CTTGAGAGATCAGAGTCCTT+AGG + chr5.4:31510289-31510308 MS.gene53091:CDS 45.0%
GAAACTGTCGGATGATCAGA+CGG + chr5.4:31508533-31508552 MS.gene53091:CDS 45.0%
GAACGAAAAGCCATCATCGA+AGG + chr5.4:31508591-31508610 MS.gene53091:CDS 45.0%
GAAGGTAAAGGAAGAAGAGC+AGG - chr5.4:31506616-31506635 None:intergenic 45.0%
GAAGTTCCTTGGGAAACTGT+CGG + chr5.4:31508521-31508540 MS.gene53091:CDS 45.0%
GATTTGGATACAAGCAGCAC+TGG - chr5.4:31509439-31509458 None:intergenic 45.0%
GCATTGCAGTTATACCTGGT+AGG - chr5.4:31508639-31508658 None:intergenic 45.0%
GGACGGTTAAATGAACTACG+TGG + chr5.4:31508837-31508856 MS.gene53091:intron 45.0%
GGCCATGCTTGCAAATTAGT+AGG + chr5.4:31508448-31508467 MS.gene53091:CDS 45.0%
GTGAAGGACAGAAGTTCCTT+GGG + chr5.4:31508511-31508530 MS.gene53091:CDS 45.0%
GTTCAGAGATACCGTGTCTA+TGG + chr5.4:31508312-31508331 MS.gene53091:CDS 45.0%
GTTGTGCGTCGAAGATTGAT+CGG - chr5.4:31509984-31510003 None:intergenic 45.0%
GTTTCGAAGATTGACGGGAA+GGG + chr5.4:31507877-31507896 MS.gene53091:CDS 45.0%
TACAACACCGACACTTGTCT+CGG - chr5.4:31509414-31509433 None:intergenic 45.0%
TCCTGGTTACCACTGTCAAA+AGG - chr5.4:31508939-31508958 None:intergenic 45.0%
TGAGCAAAGAAGGTGACGAA+GGG + chr5.4:31507914-31507933 MS.gene53091:CDS 45.0%
TGTAGATAATCCTGCGGAAG+CGG - chr5.4:31507943-31507962 None:intergenic 45.0%
TGTAGTCATTGGGGTGTGAA+GGG + chr5.4:31510091-31510110 MS.gene53091:CDS 45.0%
TGTCTATGGCTTAACTGAGG+AGG + chr5.4:31508326-31508345 MS.gene53091:CDS 45.0%
TGTGAAGGACAGAAGTTCCT+TGG + chr5.4:31508510-31508529 MS.gene53091:CDS 45.0%
TTAGGTAGAGGTGGTGTCTT+CGG - chr5.4:31510355-31510374 None:intergenic 45.0%
TTGAGCAAAGAAGGTGACGA+AGG + chr5.4:31507913-31507932 MS.gene53091:CDS 45.0%
TTGGCAAGGAGAGAAATCAC+AGG - chr5.4:31507559-31507578 None:intergenic 45.0%
TTTCGAAGATTGACGGGAAG+GGG + chr5.4:31507878-31507897 MS.gene53091:CDS 45.0%
! ACAAGGACTTGCTCTCAATG+TGG + chr5.4:31508113-31508132 MS.gene53091:CDS 45.0%
! AGAGCAAGTCCTTGTTGTGT+TGG - chr5.4:31508108-31508127 None:intergenic 45.0%
! ATGATGGCTTTTCGTTCTCC+CGG - chr5.4:31508588-31508607 None:intergenic 45.0%
! GAGCTTTGTGTGATCTGTGA+AGG + chr5.4:31508495-31508514 MS.gene53091:CDS 45.0%
! GAGTTGTCTGCCTTTGAGAA+AGG - chr5.4:31508215-31508234 None:intergenic 45.0%
! TCCTTTTGACAGTGGTAACC+AGG + chr5.4:31508935-31508954 MS.gene53091:intron 45.0%
! TCGAGGCGTTTCTGCAAATA+CGG - chr5.4:31508180-31508199 None:intergenic 45.0%
!! CTGTTGTTGGTAGCATGAAC+TGG + chr5.4:31509657-31509676 MS.gene53091:CDS 45.0%
ACAGAATCAACCACAGTCCC+TGG - chr5.4:31510037-31510056 None:intergenic 50.0%
CCGTGTCTATGGCTTAACTG+AGG + chr5.4:31508323-31508342 MS.gene53091:CDS 50.0%
CCTTCTTGTCCACCAGAATC+TGG - chr5.4:31507535-31507554 None:intergenic 50.0%
CGAATTGCCGAGACAAGTGT+CGG + chr5.4:31509404-31509423 MS.gene53091:CDS 50.0%
CGCCAGTGGAACTTTCTAGA+TGG + chr5.4:31509083-31509102 MS.gene53091:CDS 50.0%
CTTGAATCGTCGAGAGGATG+AGG - chr5.4:31509623-31509642 None:intergenic 50.0%
GAGGAAATGTTGGTCCTACC+AGG + chr5.4:31508622-31508641 MS.gene53091:CDS 50.0%
GAGTTTCGCTAACACCGTCT+CGG - chr5.4:31509826-31509845 None:intergenic 50.0%
GATCATCCGACAGTTTCCCA+AGG - chr5.4:31508530-31508549 None:intergenic 50.0%
GCAACATCCTTAGAGGGATG+AGG - chr5.4:31507643-31507662 None:intergenic 50.0%
GGCCATCTAGAAAGTTCCAC+TGG - chr5.4:31509088-31509107 None:intergenic 50.0%
GGCGTCATGAGAGGAAATGT+TGG + chr5.4:31508612-31508631 MS.gene53091:CDS 50.0%
GGGAGAAGCAAAGATATCGG+TGG + chr5.4:31508042-31508061 MS.gene53091:CDS 50.0%
GTTGCAAGGAGACCAGATTC+TGG + chr5.4:31507520-31507539 MS.gene53091:CDS 50.0%
TCAGCTCCCATGAACATCAC+CGG - chr5.4:31509590-31509609 None:intergenic 50.0%
TCCAACCATTGCACCGAGAT+CGG - chr5.4:31509744-31509763 None:intergenic 50.0%
TCTCATGACGCCTTCGATGA+TGG - chr5.4:31508604-31508623 None:intergenic 50.0%
! CCAGATTCTGGTGGACAAGA+AGG + chr5.4:31507532-31507551 MS.gene53091:CDS 50.0%
! GATGGACTTGAATCGTCGAG+AGG - chr5.4:31509629-31509648 None:intergenic 50.0%
! GATTTTATCGGGCGCTTTGC+CGG + chr5.4:31507702-31507721 MS.gene53091:CDS 50.0%
! TATTGCCGATCTCGGTGCAA+TGG + chr5.4:31509736-31509755 MS.gene53091:CDS 50.0%
!! AAGATGGGCTGTCAAAGACC+GGG + chr5.4:31508567-31508586 MS.gene53091:CDS 50.0%
!! ATCATCGAAGGCGTCATGAG+AGG + chr5.4:31508603-31508622 MS.gene53091:CDS 50.0%
!! CAAGATGGGCTGTCAAAGAC+CGG + chr5.4:31508566-31508585 MS.gene53091:CDS 50.0%
!! GAAGATTGATCGGTGTCAGC+AGG - chr5.4:31509974-31509993 None:intergenic 50.0%
!! GGTGACGTCGATTTGCTAGT+TGG - chr5.4:31507808-31507827 None:intergenic 50.0%
CCAACAACAGCAGCGACAGA+TGG - chr5.4:31509647-31509666 None:intergenic 55.0%
CCATCTGTCGCTGCTGTTGT+TGG + chr5.4:31509644-31509663 MS.gene53091:CDS 55.0%
GAATCAACCACAGTCCCTGG+TGG - chr5.4:31510034-31510053 None:intergenic 55.0%
GACGAGCCGGTGATGTTCAT+GGG + chr5.4:31509581-31509600 MS.gene53091:CDS 55.0%
GATATCGGTGGAGGAGCTGT+TGG + chr5.4:31508054-31508073 MS.gene53091:CDS 55.0%
GCAAGGAGACCAGATTCTGG+TGG + chr5.4:31507523-31507542 MS.gene53091:CDS 55.0%
GCGCTTTGCCGGCATATGAT+GGG + chr5.4:31507713-31507732 MS.gene53091:CDS 55.0%
GGAGGAGCTGTTGGATTGAG+AGG + chr5.4:31508063-31508082 MS.gene53091:CDS 55.0%
GGGGTGTGAAGGGAACAAGT+AGG + chr5.4:31510101-31510120 MS.gene53091:CDS 55.0%
TGACGAGCCGGTGATGTTCA+TGG + chr5.4:31509580-31509599 MS.gene53091:CDS 55.0%
! CTTTGCCGGCATATGATGGG+AGG + chr5.4:31507716-31507735 MS.gene53091:CDS 55.0%
CACTGGCGATCGTGACGTGA+AGG - chr5.4:31509071-31509090 None:intergenic 60.0%
CTTCACGTCACGATCGCCAG+TGG + chr5.4:31509069-31509088 MS.gene53091:CDS 60.0%
GGCGCTTTGCCGGCATATGA+TGG + chr5.4:31507712-31507731 MS.gene53091:CDS 60.0%
! GCCGATCTCGGTGCAATGGT+TGG + chr5.4:31509740-31509759 MS.gene53091:CDS 60.0%
CGAAGGGATTCCGCTTCCGC+AGG + chr5.4:31507930-31507949 MS.gene53091:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 31506406 31510414 31506406 ID=MS.gene53091
chr5.4 mRNA 31506406 31510414 31506406 ID=MS.gene53091.t1;Parent=MS.gene53091
chr5.4 exon 31506406 31506763 31506406 ID=MS.gene53091.t1.exon1;Parent=MS.gene53091.t1
chr5.4 CDS 31506406 31506763 31506406 ID=cds.MS.gene53091.t1;Parent=MS.gene53091.t1
chr5.4 exon 31507428 31508643 31507428 ID=MS.gene53091.t1.exon2;Parent=MS.gene53091.t1
chr5.4 CDS 31507428 31508643 31507428 ID=cds.MS.gene53091.t1;Parent=MS.gene53091.t1
chr5.4 exon 31508947 31510414 31508947 ID=MS.gene53091.t1.exon3;Parent=MS.gene53091.t1
chr5.4 CDS 31508947 31510414 31508947 ID=cds.MS.gene53091.t1;Parent=MS.gene53091.t1
Gene Sequence

>MS.gene53091

ATGGAACAACAAGAAAACTCAACCAACAAAAACACTAACCAAAATCTCACAAACAAAAATGGAACAAACTCTCATCAACATCATCATCATTACTATCATCACCCTCAACAGCAACAACAACAGTATCAGTATCAGAATCATCTTCAACAATACCAAACTCAACTTGGTTTCTACAACAACTACCAAAACAAGTTTCAAAGATATTACCCTGCTCTTCTTCCTTTACCTTCACTTCAACAACTACCTTTGATTCCATCTTTCCCTCAAAATCTCAAAACCCATTTGCACAAACTTCCATGCAAACTCAATACTTCTCCTTCATCTGAATACAATCTTTCTCAACTTTCACTTGATCCTGGTAAGTAAGCACCATTCTTCACTGTTCTTTTCATTTCAAACTTACCCTTTTGCTTGCATTCCTTGTTTTTTTTAATATAGTTGCTAAAAATTTGTTCTTTAAGCCTTGTTTGTTTGGTTTTTTAAGAAAAAATTTTAAATTTACTTTGACTTATTGGAGAGTGTGAAAAAAATAAATTTTTTATTGTTTCTTACTCTTTTTATATACTTTTTAAACATTGCTTTTGGGGTTGTAATAATTTATGATTTGTTTATATATTGTGCATTTTTTTAACATTGGTTTTGCTGTTTTTAATGTGAAAATGTAAGTTCTTGTTTGGGGGAATACTTTAAGGTCACTGTTTCACTGTAATACATTGTTTTGTAACTTGAGTGTTATGTTTTTGCTTAGTGATGTTTTTTTGTTAAAAGTAGATTTAATTTAAAGTGAAACGATCACTTGTTTGGTCTCACATCAAAGTCAAAATTGATTTATTCTCAAACTTGCTTTAATAAGAAGCAACAATAATTTGCTTTTTGCTTTAAGCACGTTTAGAAATTATCCTAATAACATTCTTCACATAAGATAAATCTAACTGTGACATGAAAATGATACTTAGTTATTTGAGCTTAACAATTAATTTATGCAAAAATGATGAACCATCTTATTTATGATAATGATTTAGCACCAAAGGAAATTCAGAAGCAAACTAGACCATCCTTTAAAGAAGTTGATGGGAAGAAGCTTATTTCAACAAGGAAGCCACATGAAGTAATTGTTGCAAGGAGACCAGATTCTGGTGGACAAGAAGGTCCTGTGATTTCTCTCCTTGCCAACCATTTTTTGGTGAAGTTTGATTCATCACAAAAAATATATCATTACAATGTTGAAATTACTCCTCATCCCTCTAAGGATGTTGCTAGAGAAATTAAGCATAAATTGGTAAATAACAATGCTGAGATTTTATCGGGCGCTTTGCCGGCATATGATGGGAGGAAAAATCTTTATAGTCCAATTGAATTCCAAAATGATAAGCTTGAGTTCTACATAAGCCTCCCAATTCCAACTAGCAAATCGACGTCACCTTATGAGAAACGTGAACAGCATAAACTTTTTCGGATAAATATCAAACTAGTTTCGAAGATTGACGGGAAGGGGTTGACTAAATACTTGAGCAAAGAAGGTGACGAAGGGATTCCGCTTCCGCAGGATTATCTACATGCTTTGGATGTAGTTTTGAGGGAAAGTCCGACTGAGAAATGTATACCTGTTGGAAGGTCATTCTATTCGAGTTCAATGGGGAGAAGCAAAGATATCGGTGGAGGAGCTGTTGGATTGAGAGGCTTCTTTCAGAGTCTTAGACCAACACAACAAGGACTTGCTCTCAATGTGGATTTCTCGGTAACTGCTTTCCATGAGAGTATAGGAGTTATTCCGTATTTGCAGAAACGCCTCGAGTTTCTTAGAGACCTTTCTCAAAGGCAGACAACTCAGCTAACTTGTGAAGAAAGGAAGGAAGTAGAGAAAACGTTGAAGAACATCAGAGTCTTTGTTTGCCATAGAGAAACTGTTCAGAGATACCGTGTCTATGGCTTAACTGAGGAGGCAACTGAAAATCTTTGGTTTCCTGATAGAGATGGAAAGAATCTGAGGCTTATGAGTTACTTTAAGGATCACTATAACTATGACATTCAATTCAGGAAGTGGCCATGCTTGCAAATTAGTAGGAGTAAACCTTGTTATCTCCCTATGGAGCTTTGTGTGATCTGTGAAGGACAGAAGTTCCTTGGGAAACTGTCGGATGATCAGACGGCAAAAATACTCAAGATGGGCTGTCAAAGACCGGGAGAACGAAAAGCCATCATCGAAGGCGTCATGAGAGGAAATGTTGGTCCTACCAGGTATAACTGCAATGCACTACTTTCATATTTCTTGTTTATAGAATGCTGTGTATGATTCTTAGTACTTCTATTTTATGATCATTTATGTATTTGAGTGAAATCTCATTCTGATTCCTCATAAAAACCAGAACACTGGAGTATCTTAACCAACATCCATTCACAAACCGTGAAACCGTTTATTAAGTGATTACGTGGACGGTTAAATGAACTACGTGGCATTAACAGCGTTTAAGTGATTATTACATTGTTTTTGAATTAGTTTCTTTCTACTTTCTAATGCTTTAAAAGTAATCCTTTTGACAGTGGTAACCAGGAAAATGAATTCAAACTCCAAGTCTCAAGAGAAATGACAAAGTTGACTGGGAGAATTCTTTACCCTCCAAAACTAAAGCTTGGTGATGGAGGTCATGTGAGAAATCTGACTCCTTCACGTCACGATCGCCAGTGGAACTTTCTAGATGGCCATGTCTTTGAAGGAACTACAATTGAAAGGTGGGCACTAATAAGTTTTGGAGGCACGCCTGAGCAAAAGTCTCATATTCCAAGATTCATAAACCAGTTAACTCAAAGATGTGAACAATTAGGTATTTTTCTCAACAAGAACACAATTATTAGTCCACAGTTTGAATCAATCCAAGTTCTTAACAATGTCACAATTTTGGAATCTAAGCTCAAAAGAATCCAGAGTATTGCCTCAAACAATCTACAGCTTCTTATTTGTATAATGGAGAAAAAACACAAAGGGTATGCAGATTTGAAACGAATTGCCGAGACAAGTGTCGGTGTTGTAAGCCAGTGCTGCTTGTATCCAAATCTCATCAAATTAAGTTCACAGTTTTTGGCCAATTTGGCTCTCAAGATCAATGCCAAAGTTGGTGGATGCACTGTTGCTTTGTACAACTCGCTTCCTTCTCAATTACCGCGTCTTTTCAACATTGACGAGCCGGTGATGTTCATGGGAGCTGATGTCACGCATCCTCATCCTCTCGACGATTCAAGTCCATCTGTCGCTGCTGTTGTTGGTAGCATGAACTGGCCAACAGCAAACAAATATATTTCAAGAATAAGGTCTCAAACACACAGGCAAGAAATTATTGCCGATCTCGGTGCAATGGTTGGAGAATTGCTTGAAGATTTTTATCAAGAAGTGGAAAAACTCCCAAACCGAATCATTTTCTTCCGAGACGGTGTTAGCGAAACTCAGTTTTACAAAGTTCTGCAAGAGGAACTACAATCAATAAAACAAGCATGTTCATCTAGGTTTCATGGTTATAAACCTTTTATTACTTTTGTAGTTGTGCAAAAAAGGCATCACACAAGGTTGTTTCCTGCTGACACCGATCAATCTTCGACGCACAACAATTTTCACTTTCAATATGAAAATATTCCACCAGGGACTGTGGTTGATTCTGTGATTACTCATCCAAAGGAATTTGATTTCTATCTATGTAGTCATTGGGGTGTGAAGGGAACAAGTAGGCCAACTCATTACCATGTTTTGTGGGATGAAAATAAGTTTACTTCAGATGAACTGCAAAAGCTTGTTTACAATTTGTGTTTTACTTTTGTTAGGTGTACTAAGCCAATTTCATTAGTTCCTCCTGCATATTATGCACATTTAGCTGCATATAGAGGTAGACTCTACCTTGAGAGATCAGAGTCCTTAGGTTTGTTCAGAAGTGCTTCTACATTATCAAGAGCTGCTACGCCGAAGACACCACCTCTACCTAAACTTAGTGAAAACATCAAGAAGCTTATGTTTTACTGCTAG
Protein sequence

>MS.gene53091.t1

MEQQENSTNKNTNQNLTNKNGTNSHQHHHHYYHHPQQQQQQYQYQNHLQQYQTQLGFYNNYQNKFQRYYPALLPLPSLQQLPLIPSFPQNLKTHLHKLPCKLNTSPSSEYNLSQLSLDPAPKEIQKQTRPSFKEVDGKKLISTRKPHEVIVARRPDSGGQEGPVISLLANHFLVKFDSSQKIYHYNVEITPHPSKDVAREIKHKLVNNNAEILSGALPAYDGRKNLYSPIEFQNDKLEFYISLPIPTSKSTSPYEKREQHKLFRINIKLVSKIDGKGLTKYLSKEGDEGIPLPQDYLHALDVVLRESPTEKCIPVGRSFYSSSMGRSKDIGGGAVGLRGFFQSLRPTQQGLALNVDFSVTAFHESIGVIPYLQKRLEFLRDLSQRQTTQLTCEERKEVEKTLKNIRVFVCHRETVQRYRVYGLTEEATENLWFPDRDGKNLRLMSYFKDHYNYDIQFRKWPCLQISRSKPCYLPMELCVICEGQKFLGKLSDDQTAKILKMGCQRPGERKAIIEGVMRGNVGPTSGNQENEFKLQVSREMTKLTGRILYPPKLKLGDGGHVRNLTPSRHDRQWNFLDGHVFEGTTIERWALISFGGTPEQKSHIPRFINQLTQRCEQLGIFLNKNTIISPQFESIQVLNNVTILESKLKRIQSIASNNLQLLICIMEKKHKGYADLKRIAETSVGVVSQCCLYPNLIKLSSQFLANLALKINAKVGGCTVALYNSLPSQLPRLFNIDEPVMFMGADVTHPHPLDDSSPSVAAVVGSMNWPTANKYISRIRSQTHRQEIIADLGAMVGELLEDFYQEVEKLPNRIIFFRDGVSETQFYKVLQEELQSIKQACSSRFHGYKPFITFVVVQKRHHTRLFPADTDQSSTHNNFHFQYENIPPGTVVDSVITHPKEFDFYLCSHWGVKGTSRPTHYHVLWDENKFTSDELQKLVYNLCFTFVRCTKPISLVPPAYYAHLAAYRGRLYLERSESLGLFRSASTLSRAATPKTPPLPKLSENIKKLMFYC