AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene55886


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55886.t1 MTR_8g101900 97.021 235 7 0 1 235 1 235 5.56e-171 470
MS.gene55886.t1 MTR_8g021225 73.362 229 60 1 6 233 5 233 7.20e-122 346
MS.gene55886.t1 MTR_6g091680 70.996 231 67 0 5 235 8 238 5.36e-110 316
MS.gene55886.t1 MTR_4g085590 58.929 224 91 1 12 234 23 246 1.21e-96 282
MS.gene55886.t1 MTR_4g094925 54.626 227 102 1 9 234 16 242 4.84e-90 265
MS.gene55886.t1 MTR_4g094815 53.509 228 105 1 8 234 20 247 1.72e-89 264
MS.gene55886.t1 MTR_4g094898 54.825 228 102 1 8 234 20 247 1.05e-83 249
MS.gene55886.t1 MTR_4g094812 55.804 224 98 1 12 234 22 245 1.10e-80 242
MS.gene55886.t1 MTR_4g094368 52.232 224 103 3 12 232 5 227 4.54e-79 237
MS.gene55886.t1 MTR_4g094925 53.646 192 87 2 45 234 20 211 1.17e-74 225
MS.gene55886.t1 MTR_4g094898 55.914 186 81 1 50 234 1 186 1.21e-67 207
MS.gene55886.t1 MTR_2g070410 41.204 216 118 4 18 233 91 297 3.17e-48 160
MS.gene55886.t1 MTR_2g070410 41.860 172 96 2 18 189 91 258 3.39e-38 134
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55886.t1 AT4G26220 65.022 223 78 0 12 234 9 231 7.42e-108 310
MS.gene55886.t1 AT4G34050 59.111 225 91 1 12 235 35 259 1.38e-96 283
MS.gene55886.t1 AT4G34050 59.111 225 91 1 12 235 62 286 1.60e-96 283
MS.gene55886.t1 AT1G67980 56.637 226 93 2 12 235 10 232 3.42e-88 260
MS.gene55886.t1 AT1G24735 55.947 227 95 3 12 235 16 240 5.18e-83 248
MS.gene55886.t1 AT1G67990 53.540 226 101 2 12 235 10 233 5.78e-81 242
MS.gene55886.t1 AT1G24735 45.848 277 94 4 12 234 16 290 1.06e-74 228
MS.gene55886.t1 AT1G24735 56.989 186 77 2 50 234 11 194 5.09e-71 216
MS.gene55886.t1 AT1G67980 50.909 165 78 1 70 234 2 163 3.77e-54 171
MS.gene55886.t1 AT3G61990 42.647 204 108 4 30 233 95 289 4.52e-48 160
MS.gene55886.t1 AT3G62000 40.000 205 115 4 30 233 80 277 3.54e-46 155
MS.gene55886.t1 AT4G34050 54.472 123 55 1 113 234 25 147 7.73e-45 147
MS.gene55886.t1 AT3G62000 37.383 214 123 5 11 223 64 267 2.85e-38 136

Find 41 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTCTGAAACTACCGTTTC+TGG 0.209949 4.3:+3491854 None:intergenic
CCATGAGAGGCTTATCAAAT+TGG 0.230744 4.3:-3491936 MS.gene55886:CDS
TCAAAAGTACCTTCATTTGC+AGG 0.353092 4.3:+3492001 None:intergenic
TGCCTACGATAACACCCTTT+GGG 0.369512 4.3:-3491894 MS.gene55886:CDS
GTAATTCTTCAGTGGCTTCA+TGG 0.374269 4.3:-3492775 MS.gene55886:intron
TCTAAAAGTTTATCAAGAAC+TGG 0.389451 4.3:+3492116 None:intergenic
ACCTGATGCGGGTCAGCTAA+TGG 0.395308 4.3:-3492745 MS.gene55886:CDS
ATACAAACCTTTCCATCATA+TGG 0.399941 4.3:+3492633 None:intergenic
TTGCCTACGATAACACCCTT+TGG 0.417902 4.3:-3491895 MS.gene55886:CDS
TTTCAGAAACAAAGAGAGAA+TGG 0.425659 4.3:-3491841 MS.gene55886:CDS
GACAGAAAAGCTTATGAGAT+AGG 0.427880 4.3:-3492203 MS.gene55886:CDS
TTATCAAGAACTGGCAAAGC+TGG 0.435178 4.3:+3492125 None:intergenic
GCCATTAGCTGACCCGCATC+AGG 0.436416 4.3:+3492744 None:intergenic
TAATTCTTCAGTGGCTTCAT+GGG 0.469413 4.3:-3492774 MS.gene55886:intron
ATCAAATTGGTTAAGATCGG+TGG 0.482512 4.3:-3491923 MS.gene55886:CDS
GCACTTAACATTCCATATGA+TGG 0.489413 4.3:-3492645 MS.gene55886:CDS
AACTGTTGCCTTGCCAGAAA+CGG 0.495235 4.3:-3491867 MS.gene55886:CDS
CTTATCAAATTGGTTAAGAT+CGG 0.511198 4.3:-3491926 MS.gene55886:CDS
TTGAGAGTCTCTGCTTCTCG+TGG 0.514299 4.3:+3493037 None:intergenic
CATGGGCACTTCACCTGATG+CGG 0.516416 4.3:-3492757 MS.gene55886:CDS
TTTCAATGAAGCAGTTTCAA+AGG 0.518882 4.3:-3491801 MS.gene55886:CDS
TGAAACTACCGTTTCTGGCA+AGG 0.521241 4.3:+3491859 None:intergenic
ATGGGCACTTCACCTGATGC+GGG 0.527395 4.3:-3492756 MS.gene55886:CDS
CTGCAAAATAACTGCATTGG+TGG 0.528279 4.3:+3493186 None:intergenic
CCAATTTGATAAGCCTCTCA+TGG 0.530119 4.3:+3491936 None:intergenic
TAACATTCCATATGATGGAA+AGG 0.530971 4.3:-3492640 MS.gene55886:intron
GCAGAGCGAGGGCTTACTCA+AGG 0.558409 4.3:-3493167 MS.gene55886:intron
TGCTTCTCGTGGGTAAACAC+TGG 0.559815 4.3:+3493048 None:intergenic
GGCAACAGTTCCTCCCCAAA+GGG 0.563275 4.3:+3491880 None:intergenic
AGGCAACAGTTCCTCCCCAA+AGG 0.565907 4.3:+3491879 None:intergenic
TCAAATTGGTTAAGATCGGT+GGG 0.567728 4.3:-3491922 MS.gene55886:CDS
TGAGAGTCTCTGCTTCTCGT+GGG 0.576132 4.3:+3493038 None:intergenic
GCTCTGCAAAATAACTGCAT+TGG 0.577755 4.3:+3493183 None:intergenic
GCGACGGACTCACTATCTGC+AGG 0.587918 4.3:-3491745 MS.gene55886:CDS
ACTATTGGAATTACCATGAG+AGG 0.588635 4.3:-3491949 MS.gene55886:CDS
TTGATGCAGCCTGCAAATGA+AGG 0.599302 4.3:-3492010 MS.gene55886:intron
GCCTACGATAACACCCTTTG+GGG 0.620350 4.3:-3491893 MS.gene55886:CDS
TACGATAACACCCTTTGGGG+AGG 0.624507 4.3:-3491890 MS.gene55886:CDS
AGTTTCAAAGGATCCTCGTA+TGG 0.625984 4.3:-3491789 MS.gene55886:CDS
TCCCCAAAGGGTGTTATCGT+AGG 0.628321 4.3:+3491892 None:intergenic
ACAAAAGCAAGTTCCATACG+AGG 0.723375 4.3:+3491776 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTTAGATTAATTAAGT+AGG + chr4.3:3491849-3491868 None:intergenic 10.0%
!!! TTAATTAATCTAAAATTTTG+TGG - chr4.3:3491851-3491870 MS.gene55886:CDS 10.0%
!! AAATAACTTCTATGATTTAT+AGG + chr4.3:3491996-3492015 None:intergenic 15.0%
!! AAAATTTGATTATGCATACA+AGG - chr4.3:3492024-3492043 MS.gene55886:intron 20.0%
!! ACAATGTTAAATGAATATGT+TGG - chr4.3:3492541-3492560 MS.gene55886:intron 20.0%
!!! AAAGTATCACATATAGTTTA+TGG - chr4.3:3492365-3492384 MS.gene55886:intron 20.0%
!!! TAGTTCAAAAAACTTTTACT+TGG + chr4.3:3492398-3492417 None:intergenic 20.0%
! AACTTCTATGATTTATAGGT+TGG + chr4.3:3491992-3492011 None:intergenic 25.0%
! AAGGAAAAGAAAAACCATTA+TGG + chr4.3:3492904-3492923 None:intergenic 25.0%
! AGGAAAAGAAAAACCATTAT+GGG + chr4.3:3492903-3492922 None:intergenic 25.0%
! AGTCTTAAGAATAATTACAG+AGG + chr4.3:3491955-3491974 None:intergenic 25.0%
! ATGCTGACAAAAATAACTAT+TGG - chr4.3:3492974-3492993 MS.gene55886:intron 25.0%
! ATGTTATTGTAATTCTTCAG+TGG - chr4.3:3492155-3492174 MS.gene55886:CDS 25.0%
! CTTATCAAATTGGTTAAGAT+CGG - chr4.3:3493012-3493031 MS.gene55886:CDS 25.0%
! TCTAAAAGTTTATCAAGAAC+TGG + chr4.3:3492825-3492844 None:intergenic 25.0%
!! TTTTTGAACTAGAACAATGA+TGG - chr4.3:3492407-3492426 MS.gene55886:intron 25.0%
AGGACTTCCATTCATAAAAA+AGG - chr4.3:3492755-3492774 MS.gene55886:CDS 30.0%
ATACAAACCTTTCCATCATA+TGG + chr4.3:3492308-3492327 None:intergenic 30.0%
CTAAACAGTTACAACAAACA+TGG + chr4.3:3491816-3491835 None:intergenic 30.0%
GCTAAAAAGACAATTGAAGT+AGG - chr4.3:3492239-3492258 MS.gene55886:intron 30.0%
TAAATGTATGCAAAGAGAGA+GGG + chr4.3:3492629-3492648 None:intergenic 30.0%
TTTCAATGAAGCAGTTTCAA+AGG - chr4.3:3493137-3493156 MS.gene55886:intron 30.0%
TTTCAGAAACAAAGAGAGAA+TGG - chr4.3:3493097-3493116 MS.gene55886:intron 30.0%
! ATTTTTGTCAGCATCAACAA+AGG + chr4.3:3492968-3492987 None:intergenic 30.0%
! TAACATTCCATATGATGGAA+AGG - chr4.3:3492298-3492317 MS.gene55886:intron 30.0%
!! GAACTTGCTTTTGTTTCAAT+AGG - chr4.3:3493171-3493190 MS.gene55886:CDS 30.0%
ACTATTGGAATTACCATGAG+AGG - chr4.3:3492989-3493008 MS.gene55886:intron 35.0%
ATCAAATTGGTTAAGATCGG+TGG - chr4.3:3493015-3493034 MS.gene55886:CDS 35.0%
ATCTCCACATATATCTTTCC+AGG - chr4.3:3492867-3492886 MS.gene55886:intron 35.0%
CTAAATGTATGCAAAGAGAG+AGG + chr4.3:3492630-3492649 None:intergenic 35.0%
CTTCCATTCATAAAAAAGGC+TGG - chr4.3:3492759-3492778 MS.gene55886:CDS 35.0%
CTTCTTCTCTACCAAATTGA+CGG + chr4.3:3492502-3492521 None:intergenic 35.0%
GACAGAAAAGCTTATGAGAT+AGG - chr4.3:3492735-3492754 MS.gene55886:CDS 35.0%
GCACTTAACATTCCATATGA+TGG - chr4.3:3492293-3492312 MS.gene55886:intron 35.0%
TAATTCTTCAGTGGCTTCAT+GGG - chr4.3:3492164-3492183 MS.gene55886:CDS 35.0%
TCAAATTGGTTAAGATCGGT+GGG - chr4.3:3493016-3493035 MS.gene55886:CDS 35.0%
! ATCTCATAAGCTTTTCTGTC+TGG + chr4.3:3492735-3492754 None:intergenic 35.0%
! ATTGAAGTAGGAGTTTTCAC+TGG - chr4.3:3492251-3492270 MS.gene55886:intron 35.0%
!! TCAAAAGTACCTTCATTTGC+AGG + chr4.3:3492940-3492959 None:intergenic 35.0%
!!! ACACCAGCCTTTTTTATGAA+TGG + chr4.3:3492765-3492784 None:intergenic 35.0%
AAACCATTATGGGTCTGACT+TGG + chr4.3:3492893-3492912 None:intergenic 40.0%
ACAAAAGCAAGTTCCATACG+AGG + chr4.3:3493165-3493184 None:intergenic 40.0%
AGTTTCAAAGGATCCTCGTA+TGG - chr4.3:3493149-3493168 MS.gene55886:intron 40.0%
CCAATTTGATAAGCCTCTCA+TGG + chr4.3:3493005-3493024 None:intergenic 40.0%
CCATGAGAGGCTTATCAAAT+TGG - chr4.3:3493002-3493021 MS.gene55886:intron 40.0%
CGTCAATTTGGTAGAGAAGA+AGG - chr4.3:3492500-3492519 MS.gene55886:intron 40.0%
CTGCAAAATAACTGCATTGG+TGG + chr4.3:3491755-3491774 None:intergenic 40.0%
CTGTAGAAGCCAGTATGTAT+AGG + chr4.3:3492341-3492360 None:intergenic 40.0%
GAATCCAATCACCGTCAATT+TGG - chr4.3:3492488-3492507 MS.gene55886:intron 40.0%
GCTCTGCAAAATAACTGCAT+TGG + chr4.3:3491758-3491777 None:intergenic 40.0%
GTAATTCTTCAGTGGCTTCA+TGG - chr4.3:3492163-3492182 MS.gene55886:CDS 40.0%
TAGAAGCCAGTATGTATAGG+AGG + chr4.3:3492338-3492357 None:intergenic 40.0%
TCTACCAAATTGACGGTGAT+TGG + chr4.3:3492495-3492514 None:intergenic 40.0%
TGCTTTCCTCCTATACATAC+TGG - chr4.3:3492329-3492348 MS.gene55886:intron 40.0%
TTATCAAGAACTGGCAAAGC+TGG + chr4.3:3492816-3492835 None:intergenic 40.0%
TTGGCCTGGAAAGATATATG+TGG + chr4.3:3492874-3492893 None:intergenic 40.0%
! AGCTTTTCTGTCTGGATCTA+CGG + chr4.3:3492727-3492746 None:intergenic 40.0%
!! ATACATACTGGCTTCTACAG+TGG - chr4.3:3492341-3492360 MS.gene55886:intron 40.0%
!! GCTTTTGTTTCAATAGGCGA+CGG - chr4.3:3493177-3493196 MS.gene55886:CDS 40.0%
!! GTTTCTGAAACTACCGTTTC+TGG + chr4.3:3493087-3493106 None:intergenic 40.0%
AACTGTTGCCTTGCCAGAAA+CGG - chr4.3:3493071-3493090 MS.gene55886:intron 45.0%
ATCTACAGCAGGCAGACAAA+TGG + chr4.3:3492699-3492718 None:intergenic 45.0%
TCTACAGCAGGCAGACAAAT+GGG + chr4.3:3492698-3492717 None:intergenic 45.0%
TGACTTCTTCAGTCAGCATC+AGG - chr4.3:3492115-3492134 MS.gene55886:CDS 45.0%
TGCCTACGATAACACCCTTT+GGG - chr4.3:3493044-3493063 MS.gene55886:CDS 45.0%
TGGATGTTGCTCTTCGTAAG+TGG - chr4.3:3492427-3492446 MS.gene55886:intron 45.0%
TTGATGCAGCCTGCAAATGA+AGG - chr4.3:3492928-3492947 MS.gene55886:intron 45.0%
TTGCCTACGATAACACCCTT+TGG - chr4.3:3493043-3493062 MS.gene55886:CDS 45.0%
!! GCAGTTATTTTGCAGAGCGA+GGG - chr4.3:3491760-3491779 MS.gene55886:CDS 45.0%
!! TGAAACTACCGTTTCTGGCA+AGG + chr4.3:3493082-3493101 None:intergenic 45.0%
!! TGCAGTTATTTTGCAGAGCG+AGG - chr4.3:3491759-3491778 MS.gene55886:CDS 45.0%
AGGCCAAGTCAGACCCATAA+TGG - chr4.3:3492887-3492906 MS.gene55886:intron 50.0%
CTACAGCAGGCAGACAAATG+GGG + chr4.3:3492697-3492716 None:intergenic 50.0%
CTACGGCTGTAATCTACAGC+AGG + chr4.3:3492710-3492729 None:intergenic 50.0%
GCCTACGATAACACCCTTTG+GGG - chr4.3:3493045-3493064 MS.gene55886:CDS 50.0%
TGAGAGTCTCTGCTTCTCGT+GGG + chr4.3:3491903-3491922 None:intergenic 50.0%
TGCAGGCTGCATCAAGACAA+AGG + chr4.3:3492923-3492942 None:intergenic 50.0%
TGCTTCTCGTGGGTAAACAC+TGG + chr4.3:3491893-3491912 None:intergenic 50.0%
TTGAGAGTCTCTGCTTCTCG+TGG + chr4.3:3491904-3491923 None:intergenic 50.0%
! TACGATAACACCCTTTGGGG+AGG - chr4.3:3493048-3493067 MS.gene55886:CDS 50.0%
!! ATTATGGGTCTGACTTGGCC+TGG + chr4.3:3492888-3492907 None:intergenic 50.0%
!! TCCCCAAAGGGTGTTATCGT+AGG + chr4.3:3493049-3493068 None:intergenic 50.0%
ACCTGATGCGGGTCAGCTAA+TGG - chr4.3:3492193-3492212 MS.gene55886:CDS 55.0%
AGGCAACAGTTCCTCCCCAA+AGG + chr4.3:3493062-3493081 None:intergenic 55.0%
ATGGGCACTTCACCTGATGC+GGG - chr4.3:3492182-3492201 MS.gene55886:CDS 55.0%
CATGGGCACTTCACCTGATG+CGG - chr4.3:3492181-3492200 MS.gene55886:CDS 55.0%
GGCAACAGTTCCTCCCCAAA+GGG + chr4.3:3493061-3493080 None:intergenic 55.0%
GCAGAGCGAGGGCTTACTCA+AGG - chr4.3:3491771-3491790 MS.gene55886:CDS 60.0%
GCCATTAGCTGACCCGCATC+AGG + chr4.3:3492197-3492216 None:intergenic 60.0%
! GCGACGGACTCACTATCTGC+AGG - chr4.3:3493193-3493212 MS.gene55886:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 3491733 3493227 3491733 ID=MS.gene55886
chr4.3 mRNA 3491733 3493227 3491733 ID=MS.gene55886.t1;Parent=MS.gene55886
chr4.3 exon 3493168 3493227 3493168 ID=MS.gene55886.t1.exon1;Parent=MS.gene55886.t1
chr4.3 CDS 3493168 3493227 3493168 ID=cds.MS.gene55886.t1;Parent=MS.gene55886.t1
chr4.3 exon 3493004 3493083 3493004 ID=MS.gene55886.t1.exon2;Parent=MS.gene55886.t1
chr4.3 CDS 3493004 3493083 3493004 ID=cds.MS.gene55886.t1;Parent=MS.gene55886.t1
chr4.3 exon 3492641 3492785 3492641 ID=MS.gene55886.t1.exon3;Parent=MS.gene55886.t1
chr4.3 CDS 3492641 3492785 3492641 ID=cds.MS.gene55886.t1;Parent=MS.gene55886.t1
chr4.3 exon 3492112 3492243 3492112 ID=MS.gene55886.t1.exon4;Parent=MS.gene55886.t1
chr4.3 CDS 3492112 3492243 3492112 ID=cds.MS.gene55886.t1;Parent=MS.gene55886.t1
chr4.3 exon 3491733 3492023 3491733 ID=MS.gene55886.t1.exon5;Parent=MS.gene55886.t1
chr4.3 CDS 3491733 3492023 3491733 ID=cds.MS.gene55886.t1;Parent=MS.gene55886.t1
Gene Sequence

>MS.gene55886

ATGGACAAAATATCAGCTTCCACCAATGCAGTTATTTTGCAGAGCGAGGGCTTACTCAAGGTTTACATACACATCTCTTGCCATGTTTGTTGTAACTGTTTAGATTGATTTTTCCTACTTAATTAATCTAAAATTTTGTGGCAGTATATACTAGAAACCAGTGTTTACCCACGAGAAGCAGAGACTCTCAAAGAGCTGAGAAATGCAACAGCAAATCATCCTCTGTAATTATTCTTAAGACTGCTATACTATACATCCAACCTATAAATCATAGAAGTTATTTAACATAACAAAATTTGATTATGCATACAAGGATTTTTCAAACAAAAATAGTATGATATTATTATTATTATTATTATTATTATTTTATTCTTGTTGAACTTGACTTCTTCAGTCAGCATCAGGAAACTTTTTCTTATGTAATGTTATTGTAATTCTTCAGTGGCTTCATGGGCACTTCACCTGATGCGGGTCAGCTAATGGCTTTGCTCTTGAAACTTCTAAATGCTAAAAAGACAATTGAAGTAGGAGTTTTCACTGGATACTCTCTTCTACTCACAGCACTTAACATTCCATATGATGGAAAGGTTTGTATCTGCTTTCCTCCTATACATACTGGCTTCTACAGTGGAAAAGTATCACATATAGTTTATGGTAGATCACCAAGTAAAAGTTTTTTGAACTAGAACAATGATGGATGTTGCTCTTCGTAAGTGGAAGCTCATGATATTATGCATTCTCAAGACTGAGACGATGAATCCAATCACCGTCAATTTGGTAGAGAAGAAGGAACAAAAAAACTATTTGAACAATGTTAAATGAATATGTTGGTCATCGTAAGAGTTTTGAACAATTTCAATGCATAAGATGCTTAATTCTCACGCTAGCAATCACCCTCTCTCTTTGCATACATTTAGATAATAACGTCGCAAGTATGTGTCTATTATTTTTATGTTACTAGCCCCATTTGTCTGCCTGCTGTAGATTACAGCCGTAGATCCAGACAGAAAAGCTTATGAGATAGGACTTCCATTCATAAAAAAGGCTGGTGTCTTACACAAGATTGACTACATAGAGTCTCCAGCTTTGCCAGTTCTTGATAAACTTTTAGAAGATGTAAGCAACTTACTTAACATCTCCACATATATCTTTCCAGGCCAAGTCAGACCCATAATGGTTTTTCTTTTCCTTTGTCTTGATGCAGCCTGCAAATGAAGGTACTTTTGACTTTGCCTTTGTTGATGCTGACAAAAATAACTATTGGAATTACCATGAGAGGCTTATCAAATTGGTTAAGATCGGTGGGATTGTTGCCTACGATAACACCCTTTGGGGAGGAACTGTTGCCTTGCCAGAAACGGTAGTTTCAGAAACAAAGAGAGAATGGAGAAGATGTACACTTGATTTCAATGAAGCAGTTTCAAAGGATCCTCGTATGGAACTTGCTTTTGTTTCAATAGGCGACGGACTCACTATCTGCAGGCGCATTTGCTGA

Protein sequence

>MS.gene55886.t1

MDKISASTNAVILQSEGLLKYILETSVYPREAETLKELRNATANHPLGFMGTSPDAGQLMALLLKLLNAKKTIEVGVFTGYSLLLTALNIPYDGKITAVDPDRKAYEIGLPFIKKAGVLHKIDYIESPALPVLDKLLEDPANEGTFDFAFVDADKNNYWNYHERLIKLVKIGGIVAYDNTLWGGTVALPETVVSETKREWRRCTLDFNEAVSKDPRMELAFVSIGDGLTICRRIC