Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57518.t1 | XP_013444914.1 | 89.7 | 184 | 18 | 1 | 1 | 183 | 1 | 184 | 4.50E-88 | 334 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57518.t1 | A0A072TN78 | 89.7 | 184 | 18 | 1 | 1 | 183 | 1 | 184 | 3.3e-88 | 334.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene57518 | 0.81255 | 4.01E-51 | -1.69E-46 |
| MS.gene050945 | MS.gene57518 | 0.816268 | 6.04E-52 | -1.69E-46 |
| MS.gene051960 | MS.gene57518 | 0.804278 | 2.33E-49 | -1.69E-46 |
| MS.gene053879 | MS.gene57518 | 0.818338 | 2.07E-52 | -1.69E-46 |
| MS.gene054892 | MS.gene57518 | 0.807219 | 5.62E-50 | -1.69E-46 |
| MS.gene054893 | MS.gene57518 | 0.810909 | 9.12E-51 | -1.69E-46 |
| MS.gene055150 | MS.gene57518 | 0.832663 | 8.31E-56 | -1.69E-46 |
| MS.gene055886 | MS.gene57518 | 0.818722 | 1.69E-52 | -1.69E-46 |
| MS.gene057174 | MS.gene57518 | 0.808878 | 2.49E-50 | -1.69E-46 |
| MS.gene057622 | MS.gene57518 | 0.808205 | 3.47E-50 | -1.69E-46 |
| MS.gene05952 | MS.gene57518 | 0.817516 | 3.17E-52 | -1.69E-46 |
| MS.gene05985 | MS.gene57518 | -0.812896 | 3.37E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57518.t1 | MTR_8g432440 | 89.674 | 184 | 18 | 1 | 1 | 183 | 1 | 184 | 4.35e-121 | 340 |
| MS.gene57518.t1 | MTR_8g432420 | 89.674 | 184 | 18 | 1 | 1 | 183 | 1 | 184 | 4.35e-121 | 340 |
| MS.gene57518.t1 | MTR_8g432460 | 88.525 | 183 | 21 | 0 | 1 | 183 | 1 | 183 | 5.12e-120 | 337 |
| MS.gene57518.t1 | MTR_8g432470 | 85.870 | 184 | 25 | 1 | 1 | 183 | 1 | 184 | 7.33e-115 | 324 |
| MS.gene57518.t1 | MTR_8g432490 | 83.607 | 183 | 30 | 0 | 1 | 183 | 1 | 183 | 1.92e-112 | 318 |
| MS.gene57518.t1 | MTR_3g064780 | 80.556 | 180 | 35 | 0 | 1 | 180 | 1 | 180 | 2.22e-106 | 305 |
| MS.gene57518.t1 | MTR_8g432450 | 84.746 | 177 | 25 | 2 | 1 | 175 | 1 | 177 | 1.26e-105 | 300 |
| MS.gene57518.t1 | MTR_8g432480 | 54.545 | 176 | 58 | 6 | 5 | 174 | 7 | 166 | 3.87e-50 | 160 |
| MS.gene57518.t1 | MTR_7g076000 | 34.637 | 179 | 89 | 6 | 5 | 176 | 2 | 159 | 5.78e-24 | 92.8 |
| MS.gene57518.t1 | MTR_8g432400 | 34.426 | 183 | 103 | 6 | 5 | 179 | 2 | 175 | 5.25e-22 | 88.2 |
| MS.gene57518.t1 | MTR_8g432370 | 34.783 | 184 | 100 | 7 | 5 | 179 | 2 | 174 | 1.69e-21 | 86.7 |
| MS.gene57518.t1 | MTR_8g432350 | 34.615 | 182 | 99 | 7 | 5 | 177 | 2 | 172 | 1.89e-20 | 84.0 |
| MS.gene57518.t1 | MTR_8g432390 | 34.595 | 185 | 100 | 8 | 5 | 179 | 2 | 175 | 1.95e-19 | 81.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 49 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACTCTAATGGTGTTGTTAT+TGG | 0.174838 | 8.3:-61889977 | MS.gene57518:CDS |
| TGAGTTATAAATTGAGGAAT+TGG | 0.246315 | 8.3:-61890144 | MS.gene57518:CDS |
| AACAACACCATTAGAGTTCT+TGG | 0.251508 | 8.3:+61889982 | None:intergenic |
| AATTATGGTGGTCGGTTAAC+TGG | 0.256041 | 8.3:-61890019 | MS.gene57518:CDS |
| ATAAATATTTAAATAATGTT+TGG | 0.290889 | 8.3:+61889932 | None:intergenic |
| TCAAAGGTATTCGACCTATT+GGG | 0.300168 | 8.3:-61890231 | MS.gene57518:CDS |
| TTCAAAGGTATTCGACCTAT+TGG | 0.303554 | 8.3:-61890232 | MS.gene57518:CDS |
| TTAGAAATTACGGTTATATC+TGG | 0.325832 | 8.3:-61890448 | MS.gene57518:CDS |
| TTCACAAACATTAGAAATTA+CGG | 0.327531 | 8.3:-61890458 | MS.gene57518:CDS |
| AATTGGGAAGGGAGGCAAAA+TGG | 0.332260 | 8.3:-61890127 | MS.gene57518:CDS |
| TAGTGTGAGGGTGGTTGTTC+CGG | 0.338986 | 8.3:-61890092 | MS.gene57518:CDS |
| TAAATATTTAAATAATGTTT+GGG | 0.348234 | 8.3:+61889933 | None:intergenic |
| GTGGCGGATGGAAAGAATTA+TGG | 0.368827 | 8.3:-61890034 | MS.gene57518:CDS |
| TTGTTATTGGTATTCCGTTT+TGG | 0.371495 | 8.3:-61889964 | MS.gene57518:CDS |
| GCAATTCTTGCCACCCCAAT+AGG | 0.391470 | 8.3:+61890217 | None:intergenic |
| GGTGGCAAGAATTGCTCTTT+CGG | 0.416259 | 8.3:-61890209 | MS.gene57518:CDS |
| CACCGTTTGCTTCTCCGCCT+CGG | 0.445504 | 8.3:+61890054 | None:intergenic |
| GAGTTATAAATTGAGGAATT+GGG | 0.456017 | 8.3:-61890143 | MS.gene57518:CDS |
| TATTCTCAATGGCGCGGTAT+CGG | 0.469676 | 8.3:-61890185 | MS.gene57518:CDS |
| GCTCTTTCGGATATTCTCAA+TGG | 0.472815 | 8.3:-61890196 | MS.gene57518:CDS |
| TGAAGGAAAATGATAATTGT+GGG | 0.480545 | 8.3:-61890348 | MS.gene57518:CDS |
| GGCGGAGAAGCAAACGGTGG+CGG | 0.482611 | 8.3:-61890050 | MS.gene57518:CDS |
| GTGAAGGAAAATGATAATTG+TGG | 0.484213 | 8.3:-61890349 | MS.gene57518:CDS |
| TTTGGGTAATTCCACCAAAA+CGG | 0.496948 | 8.3:+61889950 | None:intergenic |
| TGCAAGGTCAATCACGTTTG+AGG | 0.523501 | 8.3:-61890269 | MS.gene57518:CDS |
| CAAAGGTATTCGACCTATTG+GGG | 0.532466 | 8.3:-61890230 | MS.gene57518:CDS |
| CGGTTAACTGGAATGGATGT+TGG | 0.534432 | 8.3:-61890007 | MS.gene57518:CDS |
| GAGAAGCAAACGGTGGCGGA+TGG | 0.538736 | 8.3:-61890046 | MS.gene57518:CDS |
| TGGTGGTCGGTTAACTGGAA+TGG | 0.553085 | 8.3:-61890014 | MS.gene57518:CDS |
| GTTGGTGCCAAGAACTCTAA+TGG | 0.557347 | 8.3:-61889989 | MS.gene57518:CDS |
| TCGGTATCCGATCTTTGCTC+CGG | 0.559233 | 8.3:+61890073 | None:intergenic |
| AATTGAGGAATTGGGAAGGG+AGG | 0.560763 | 8.3:-61890135 | MS.gene57518:CDS |
| GGTTGTTCCGGAGCAAAGAT+CGG | 0.564026 | 8.3:-61890080 | MS.gene57518:CDS |
| TGAACATGCCATTGCATGCA+AGG | 0.566598 | 8.3:-61890285 | MS.gene57518:CDS |
| TATAAATTGAGGAATTGGGA+AGG | 0.569289 | 8.3:-61890139 | MS.gene57518:CDS |
| ATAAATTGAGGAATTGGGAA+GGG | 0.583144 | 8.3:-61890138 | MS.gene57518:CDS |
| TACCGAGGCGGAGAAGCAAA+CGG | 0.584351 | 8.3:-61890056 | MS.gene57518:CDS |
| GTGCAATGCAAGAAGTTCAA+AGG | 0.594547 | 8.3:-61890247 | MS.gene57518:CDS |
| TTCGGATATTCTCAATGGCG+CGG | 0.599946 | 8.3:-61890191 | MS.gene57518:CDS |
| GTGATTGACCTTGCATGCAA+TGG | 0.618595 | 8.3:+61890277 | None:intergenic |
| CGAAAATATTCATGTAACAG+AGG | 0.621033 | 8.3:-61890425 | MS.gene57518:CDS |
| ATGGAAAGAATTATGGTGGT+CGG | 0.623818 | 8.3:-61890027 | MS.gene57518:CDS |
| GATGCATATGTTGTTGTTCG+AGG | 0.632034 | 8.3:-61890403 | MS.gene57518:CDS |
| GCGGATGGAAAGAATTATGG+TGG | 0.668878 | 8.3:-61890031 | MS.gene57518:CDS |
| AGGTATTCGACCTATTGGGG+TGG | 0.674947 | 8.3:-61890227 | MS.gene57518:CDS |
| GCAAAGATCGGATACCGAGG+CGG | 0.694888 | 8.3:-61890068 | MS.gene57518:CDS |
| TGTTTGTGAAGTCACAACCA+TGG | 0.699218 | 8.3:+61890471 | None:intergenic |
| GGAGCAAAGATCGGATACCG+AGG | 0.705796 | 8.3:-61890071 | MS.gene57518:CDS |
| CGAGGCGGAGAAGCAAACGG+TGG | 0.746726 | 8.3:-61890053 | MS.gene57518:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATAGTTACACAACAAAAA+CGG | - | chr8.3:61890038-61890057 | MS.gene57518:CDS | 20.0% |
| !! | TTCACAAACATTAGAAATTA+CGG | - | chr8.3:61889951-61889970 | MS.gene57518:CDS | 20.0% |
| !!! | GAATTTTGAGTTATAAATTG+AGG | - | chr8.3:61890259-61890278 | MS.gene57518:CDS | 20.0% |
| ! | GAGTTATAAATTGAGGAATT+GGG | - | chr8.3:61890266-61890285 | MS.gene57518:CDS | 25.0% |
| ! | TGAAGGAAAATGATAATTGT+GGG | - | chr8.3:61890061-61890080 | MS.gene57518:CDS | 25.0% |
| ! | TGAGTTATAAATTGAGGAAT+TGG | - | chr8.3:61890265-61890284 | MS.gene57518:CDS | 25.0% |
| ! | TTAGAAATTACGGTTATATC+TGG | - | chr8.3:61889961-61889980 | MS.gene57518:CDS | 25.0% |
| !!! | AAAACTCGAGTTTTATTTCA+TGG | - | chr8.3:61890085-61890104 | MS.gene57518:CDS | 25.0% |
| !!! | AGTTATTCATTTTAGTGTGA+GGG | - | chr8.3:61890305-61890324 | MS.gene57518:CDS | 25.0% |
| ATAAATTGAGGAATTGGGAA+GGG | - | chr8.3:61890271-61890290 | MS.gene57518:CDS | 30.0% | |
| CGAAAATATTCATGTAACAG+AGG | - | chr8.3:61889984-61890003 | MS.gene57518:CDS | 30.0% | |
| GTGAAGGAAAATGATAATTG+TGG | - | chr8.3:61890060-61890079 | MS.gene57518:CDS | 30.0% | |
| TATAAATTGAGGAATTGGGA+AGG | - | chr8.3:61890270-61890289 | MS.gene57518:CDS | 30.0% | |
| !! | AACTCTAATGGTGTTGTTAT+TGG | - | chr8.3:61890432-61890451 | MS.gene57518:CDS | 30.0% |
| !! | GAGTTATTCATTTTAGTGTG+AGG | - | chr8.3:61890304-61890323 | MS.gene57518:CDS | 30.0% |
| !! | TTGTTATTGGTATTCCGTTT+TGG | - | chr8.3:61890445-61890464 | MS.gene57518:CDS | 30.0% |
| AACAACACCATTAGAGTTCT+TGG | + | chr8.3:61890430-61890449 | None:intergenic | 35.0% | |
| ATGGAAAGAATTATGGTGGT+CGG | - | chr8.3:61890382-61890401 | MS.gene57518:CDS | 35.0% | |
| TTACACAACAAAAACGGTGA+AGG | - | chr8.3:61890044-61890063 | MS.gene57518:CDS | 35.0% | |
| TTTGGGTAATTCCACCAAAA+CGG | + | chr8.3:61890462-61890481 | None:intergenic | 35.0% | |
| ! | TTATTGGTATTCCGTTTTGG+TGG | - | chr8.3:61890448-61890467 | MS.gene57518:CDS | 35.0% |
| !! | TATTCATTTTAGTGTGAGGG+TGG | - | chr8.3:61890308-61890327 | MS.gene57518:CDS | 35.0% |
| !! | TCAAAGGTATTCGACCTATT+GGG | - | chr8.3:61890178-61890197 | MS.gene57518:CDS | 35.0% |
| !! | TTCAAAGGTATTCGACCTAT+TGG | - | chr8.3:61890177-61890196 | MS.gene57518:CDS | 35.0% |
| AATTATGGTGGTCGGTTAAC+TGG | - | chr8.3:61890390-61890409 | MS.gene57518:CDS | 40.0% | |
| GCTCTTTCGGATATTCTCAA+TGG | - | chr8.3:61890213-61890232 | MS.gene57518:CDS | 40.0% | |
| GTGCAATGCAAGAAGTTCAA+AGG | - | chr8.3:61890162-61890181 | MS.gene57518:CDS | 40.0% | |
| ! | GATGCATATGTTGTTGTTCG+AGG | - | chr8.3:61890006-61890025 | MS.gene57518:CDS | 40.0% |
| !! | CAAAGGTATTCGACCTATTG+GGG | - | chr8.3:61890179-61890198 | MS.gene57518:CDS | 40.0% |
| AATTGAGGAATTGGGAAGGG+AGG | - | chr8.3:61890274-61890293 | MS.gene57518:CDS | 45.0% | |
| AATTGGGAAGGGAGGCAAAA+TGG | - | chr8.3:61890282-61890301 | MS.gene57518:CDS | 45.0% | |
| CGGTTAACTGGAATGGATGT+TGG | - | chr8.3:61890402-61890421 | MS.gene57518:CDS | 45.0% | |
| GCGGATGGAAAGAATTATGG+TGG | - | chr8.3:61890378-61890397 | MS.gene57518:CDS | 45.0% | |
| GGTGGCAAGAATTGCTCTTT+CGG | - | chr8.3:61890200-61890219 | MS.gene57518:CDS | 45.0% | |
| GTGATTGACCTTGCATGCAA+TGG | + | chr8.3:61890135-61890154 | None:intergenic | 45.0% | |
| TATTCTCAATGGCGCGGTAT+CGG | - | chr8.3:61890224-61890243 | MS.gene57518:CDS | 45.0% | |
| TGAACATGCCATTGCATGCA+AGG | - | chr8.3:61890124-61890143 | MS.gene57518:CDS | 45.0% | |
| TGCAAGGTCAATCACGTTTG+AGG | - | chr8.3:61890140-61890159 | MS.gene57518:CDS | 45.0% | |
| TTCGGATATTCTCAATGGCG+CGG | - | chr8.3:61890218-61890237 | MS.gene57518:CDS | 45.0% | |
| !! | GTGGCGGATGGAAAGAATTA+TGG | - | chr8.3:61890375-61890394 | MS.gene57518:CDS | 45.0% |
| !! | GTTGGTGCCAAGAACTCTAA+TGG | - | chr8.3:61890420-61890439 | MS.gene57518:CDS | 45.0% |
| AGGTATTCGACCTATTGGGG+TGG | - | chr8.3:61890182-61890201 | MS.gene57518:CDS | 50.0% | |
| GCAATTCTTGCCACCCCAAT+AGG | + | chr8.3:61890195-61890214 | None:intergenic | 50.0% | |
| GGTTGTTCCGGAGCAAAGAT+CGG | - | chr8.3:61890329-61890348 | MS.gene57518:CDS | 50.0% | |
| TAGTGTGAGGGTGGTTGTTC+CGG | - | chr8.3:61890317-61890336 | MS.gene57518:CDS | 50.0% | |
| TCGGTATCCGATCTTTGCTC+CGG | + | chr8.3:61890339-61890358 | None:intergenic | 50.0% | |
| TGGTGGTCGGTTAACTGGAA+TGG | - | chr8.3:61890395-61890414 | MS.gene57518:CDS | 50.0% | |
| GCAAAGATCGGATACCGAGG+CGG | - | chr8.3:61890341-61890360 | MS.gene57518:CDS | 55.0% | |
| GGAGCAAAGATCGGATACCG+AGG | - | chr8.3:61890338-61890357 | MS.gene57518:CDS | 55.0% | |
| TACCGAGGCGGAGAAGCAAA+CGG | - | chr8.3:61890353-61890372 | MS.gene57518:CDS | 55.0% | |
| CACCGTTTGCTTCTCCGCCT+CGG | + | chr8.3:61890358-61890377 | None:intergenic | 60.0% | |
| !! | GAGAAGCAAACGGTGGCGGA+TGG | - | chr8.3:61890363-61890382 | MS.gene57518:CDS | 60.0% |
| CGAGGCGGAGAAGCAAACGG+TGG | - | chr8.3:61890356-61890375 | MS.gene57518:CDS | 65.0% | |
| GGCGGAGAAGCAAACGGTGG+CGG | - | chr8.3:61890359-61890378 | MS.gene57518:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 61889940 | 61890491 | 61889940 | ID=MS.gene57518 |
| chr8.3 | mRNA | 61889940 | 61890491 | 61889940 | ID=MS.gene57518.t1;Parent=MS.gene57518 |
| chr8.3 | exon | 61889940 | 61890491 | 61889940 | ID=MS.gene57518.t1.exon1;Parent=MS.gene57518.t1 |
| chr8.3 | CDS | 61889940 | 61890491 | 61889940 | ID=cds.MS.gene57518.t1;Parent=MS.gene57518.t1 |
| Gene Sequence |
| Protein sequence |
