Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58473.t1 | XP_003609618.1 | 91.6 | 83 | 7 | 0 | 1 | 83 | 289 | 371 | 1.00E-34 | 155.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58473.t1 | Q8GWP5 | 44.1 | 68 | 38 | 0 | 15 | 82 | 303 | 370 | 5.6e-10 | 64.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58473.t1 | G7JK95 | 91.6 | 83 | 7 | 0 | 1 | 83 | 289 | 371 | 7.2e-35 | 155.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049378 | MS.gene58473 | 0.817129 | 3.87E-52 | -1.69E-46 |
| MS.gene049758 | MS.gene58473 | 0.817803 | 2.73E-52 | -1.69E-46 |
| MS.gene04984 | MS.gene58473 | 0.826577 | 2.51E-54 | -1.69E-46 |
| MS.gene049915 | MS.gene58473 | 0.828487 | 8.75E-55 | -1.69E-46 |
| MS.gene050226 | MS.gene58473 | 0.823018 | 1.74E-53 | -1.69E-46 |
| MS.gene050227 | MS.gene58473 | 0.826667 | 2.39E-54 | -1.69E-46 |
| MS.gene050228 | MS.gene58473 | 0.819851 | 9.35E-53 | -1.69E-46 |
| MS.gene050642 | MS.gene58473 | 0.801302 | 9.60E-49 | -1.69E-46 |
| MS.gene050719 | MS.gene58473 | 0.808427 | 3.11E-50 | -1.69E-46 |
| MS.gene050721 | MS.gene58473 | 0.815736 | 7.94E-52 | -1.69E-46 |
| MS.gene050724 | MS.gene58473 | 0.817561 | 3.09E-52 | -1.69E-46 |
| MS.gene052176 | MS.gene58473 | 0.818941 | 1.51E-52 | -1.69E-46 |
| MS.gene052376 | MS.gene58473 | 0.81395 | 1.97E-51 | -1.69E-46 |
| MS.gene052856 | MS.gene58473 | 0.805046 | 1.61E-49 | -1.69E-46 |
| MS.gene053836 | MS.gene58473 | 0.821335 | 4.27E-53 | -1.69E-46 |
| MS.gene054930 | MS.gene58473 | 0.81481 | 1.27E-51 | -1.69E-46 |
| MS.gene058168 | MS.gene58473 | 0.801132 | 1.04E-48 | -1.69E-46 |
| MS.gene058182 | MS.gene58473 | 0.801518 | 8.67E-49 | -1.69E-46 |
| MS.gene058710 | MS.gene58473 | 0.804358 | 2.24E-49 | -1.69E-46 |
| MS.gene058749 | MS.gene58473 | 0.803976 | 2.70E-49 | -1.69E-46 |
| MS.gene059124 | MS.gene58473 | 0.809091 | 2.25E-50 | -1.69E-46 |
| MS.gene05984 | MS.gene58473 | 0.804393 | 2.21E-49 | -1.69E-46 |
| MS.gene060404 | MS.gene58473 | 0.800442 | 1.44E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58473.t1 | MTR_4g119220 | 91.566 | 83 | 7 | 0 | 1 | 83 | 289 | 371 | 5.51e-49 | 160 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58473.t1 | AT4G20960 | 44.118 | 68 | 38 | 0 | 15 | 82 | 303 | 370 | 7.21e-14 | 65.1 |
Find 13 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTCCTTGTTGAGCTTCTTC+TGG | 0.220389 | 8.1:+8771366 | None:intergenic |
| AGGAATTGAAATTGTATCTA+TGG | 0.284535 | 8.1:-8771349 | MS.gene58473:CDS |
| GTGAATATGAAGAGCTTGTT+AGG | 0.298078 | 8.1:-8771242 | MS.gene58473:CDS |
| AATAAATCTGGATGTGATCT+TGG | 0.364059 | 8.1:-8771322 | MS.gene58473:CDS |
| ATCTATGGATCAAATAAATC+TGG | 0.375725 | 8.1:-8771334 | MS.gene58473:CDS |
| AATTCCCTCTCACATCTAAC+AGG | 0.424466 | 8.1:+8771268 | None:intergenic |
| TTGGATTATTGTAATCATCA+AGG | 0.474418 | 8.1:-8771303 | MS.gene58473:CDS |
| ATATGAAGAGCTTGTTAGGA+AGG | 0.535431 | 8.1:-8771238 | None:intergenic |
| TTCTTCAAATCAAATTCCAT+CGG | 0.538341 | 8.1:-8771448 | MS.gene58473:CDS |
| GTAACTTCATTGATGACCGA+TGG | 0.596622 | 8.1:+8771432 | None:intergenic |
| AGTGTCCTGTTAGATGTGAG+AGG | 0.685168 | 8.1:-8771273 | MS.gene58473:CDS |
| ACTCCAGAAGAAGCTCAACA+AGG | 0.687391 | 8.1:-8771369 | MS.gene58473:CDS |
| GTGTCCTGTTAGATGTGAGA+GGG | 0.689300 | 8.1:-8771272 | MS.gene58473:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGTAAAAATTATCGCTTTAT+CGG | + | chr8.1:8771297-8771316 | None:intergenic | 20.0% |
| ! | AGGAATTGAAATTGTATCTA+TGG | - | chr8.1:8771355-8771374 | MS.gene58473:CDS | 25.0% |
| ! | ATCTATGGATCAAATAAATC+TGG | - | chr8.1:8771370-8771389 | MS.gene58473:CDS | 25.0% |
| ! | TTCTTCAAATCAAATTCCAT+CGG | - | chr8.1:8771256-8771275 | MS.gene58473:CDS | 25.0% |
| ! | TTGGATTATTGTAATCATCA+AGG | - | chr8.1:8771401-8771420 | MS.gene58473:CDS | 25.0% |
| AATAAATCTGGATGTGATCT+TGG | - | chr8.1:8771382-8771401 | MS.gene58473:CDS | 30.0% | |
| ! | GTGAATATGAAGAGCTTGTT+AGG | - | chr8.1:8771462-8771481 | MS.gene58473:CDS | 35.0% |
| AATTCCCTCTCACATCTAAC+AGG | + | chr8.1:8771439-8771458 | None:intergenic | 40.0% | |
| ATTCCTTGTTGAGCTTCTTC+TGG | + | chr8.1:8771341-8771360 | None:intergenic | 40.0% | |
| ! | GTAACTTCATTGATGACCGA+TGG | + | chr8.1:8771275-8771294 | None:intergenic | 40.0% |
| ACTCCAGAAGAAGCTCAACA+AGG | - | chr8.1:8771335-8771354 | MS.gene58473:CDS | 45.0% | |
| AGTGTCCTGTTAGATGTGAG+AGG | - | chr8.1:8771431-8771450 | MS.gene58473:CDS | 45.0% | |
| GTGTCCTGTTAGATGTGAGA+GGG | - | chr8.1:8771432-8771451 | MS.gene58473:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 8771239 | 8771487 | 8771239 | ID=MS.gene58473 |
| chr8.1 | mRNA | 8771239 | 8771487 | 8771239 | ID=MS.gene58473.t1;Parent=MS.gene58473 |
| chr8.1 | exon | 8771239 | 8771487 | 8771239 | ID=MS.gene58473.t1.exon1;Parent=MS.gene58473.t1 |
| chr8.1 | CDS | 8771239 | 8771487 | 8771239 | ID=cds.MS.gene58473.t1;Parent=MS.gene58473.t1 |
| Gene Sequence |
| Protein sequence |
