Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59879.t1 | XP_013445262.1 | 75.2 | 383 | 70 | 1 | 1 | 383 | 54 | 411 | 9.30E-43 | 184.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59879.t1 | A0A072TR16 | 75.2 | 383 | 70 | 1 | 1 | 383 | 54 | 411 | 6.7e-43 | 184.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049698 | MS.gene59879 | 0.821484 | 3.94E-53 | -1.69E-46 |
| MS.gene05017 | MS.gene59879 | 0.815752 | 7.87E-52 | -1.69E-46 |
| MS.gene053536 | MS.gene59879 | 0.811024 | 8.61E-51 | -1.69E-46 |
| MS.gene053538 | MS.gene59879 | 0.805771 | 1.14E-49 | -1.69E-46 |
| MS.gene055144 | MS.gene59879 | 0.810332 | 1.22E-50 | -1.69E-46 |
| MS.gene055537 | MS.gene59879 | 0.811163 | 8.03E-51 | -1.69E-46 |
| MS.gene055764 | MS.gene59879 | 0.81011 | 1.36E-50 | -1.69E-46 |
| MS.gene058339 | MS.gene59879 | 0.81355 | 2.42E-51 | -1.69E-46 |
| MS.gene058351 | MS.gene59879 | 0.837891 | 3.98E-57 | -1.69E-46 |
| MS.gene058909 | MS.gene59879 | 0.847676 | 9.94E-60 | -1.69E-46 |
| MS.gene058912 | MS.gene59879 | 0.846463 | 2.14E-59 | -1.69E-46 |
| MS.gene05894 | MS.gene59879 | 0.802428 | 5.64E-49 | -1.69E-46 |
| MS.gene059424 | MS.gene59879 | 0.809148 | 2.18E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59879.t1 | MTR_8g046245 | 85.195 | 385 | 28 | 3 | 1 | 383 | 54 | 411 | 0.0 | 603 |
| MS.gene59879.t1 | MTR_3g007660 | 31.354 | 421 | 183 | 19 | 1 | 383 | 50 | 402 | 8.21e-29 | 116 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTAAATCTTTATTATTTGA+AGG | - | 54856:13961-13980 | MS.gene59879:intergenic | 15.0% |
| ! | AATTTAGAGGAAAAGATAGT+AGG | + | 54856:14498-14517 | MS.gene59879:CDS | 25.0% |
| ! | CAAAACACAAACAAATGAAA+AGG | + | 54856:14416-14435 | MS.gene59879:CDS | 25.0% |
| ! | TAAAGATTTAGATGAAGCAA+AGG | + | 54856:13969-13988 | MS.gene59879:CDS | 25.0% |
| !! | ACCAATTTCTCTATTGTTTT+TGG | - | 54856:14468-14487 | MS.gene59879:intergenic | 25.0% |
| !! | CTTTTCATCTATTTGTGTTT+TGG | - | 54856:14343-14362 | MS.gene59879:intergenic | 25.0% |
| !! | CTTTTCATTTGTTTGTGTTT+TGG | - | 54856:14418-14437 | MS.gene59879:intergenic | 25.0% |
| !! | CTTTTCCTCTAAATTTTCTT+TGG | - | 54856:14493-14512 | MS.gene59879:intergenic | 25.0% |
| !! | TCATTGCTAATTGTTTCTTT+AGG | - | 54856:14135-14154 | MS.gene59879:intergenic | 25.0% |
| !!! | AATTTTCTCCATTTGTTAAC+TGG | - | 54856:13793-13812 | MS.gene59879:intergenic | 25.0% |
| !!! | CTTTTCATCTATTTGCTTTT+TGG | - | 54856:14217-14236 | MS.gene59879:intergenic | 25.0% |
| AACAAATGAAAAGGCTCAAA+AGG | + | 54856:14425-14444 | MS.gene59879:CDS | 30.0% | |
| AATAGATGAAAAGCCTCAAA+AGG | + | 54856:14224-14243 | MS.gene59879:CDS | 30.0% | |
| AATGACAATAACCCTCAAAA+GGG | + | 54856:14150-14169 | MS.gene59879:CDS | 30.0% | |
| ACAAATGAAAAGGCTCAAAA+GGG | + | 54856:14426-14445 | MS.gene59879:CDS | 30.0% | |
| AGACATTTAGCTGAAAATGT+AGG | + | 54856:14798-14817 | MS.gene59879:CDS | 30.0% | |
| ATAGATGAAAAGCCTCAAAA+GGG | + | 54856:14225-14244 | MS.gene59879:CDS | 30.0% | |
| ATAGATGAAAAGCCTCAAAA+TGG | + | 54856:14225-14244 | MS.gene59879:CDS | 30.0% | |
| GCCAAAAACAATAGAGAAAT+TGG | + | 54856:14464-14483 | MS.gene59879:CDS | 30.0% | |
| GGTTACCAAAGAAAATTTAG+AGG | + | 54856:14485-14504 | MS.gene59879:CDS | 30.0% | |
| TCAAAATGGTAAAGAGGATT+TGG | + | 54856:14365-14384 | MS.gene59879:CDS | 30.0% | |
| ! | AAATCCTCTTTACCATTTTG+AGG | - | 54856:14366-14385 | MS.gene59879:intergenic | 30.0% |
| ! | GTTTCTTTAGGAATTTGAGA+TGG | - | 54856:14123-14142 | MS.gene59879:intergenic | 30.0% |
| !! | GTTGATTCTTCCAATTTTTG+TGG | - | 54856:14027-14046 | MS.gene59879:intergenic | 30.0% |
| !!! | CTTTTTTCTACTTCTTGTTG+AGG | - | 54856:13937-13956 | MS.gene59879:intergenic | 30.0% |
| AAAGCCTCAAAATGGTAAAG+AGG | + | 54856:14359-14378 | MS.gene59879:CDS | 35.0% | |
| AAATGTTCCAGAGAAGGAAA+AGG | + | 54856:14659-14678 | MS.gene59879:CDS | 35.0% | |
| ACTTGTGATGATTGTGAAGT+TGG | - | 54856:13910-13929 | MS.gene59879:intergenic | 35.0% | |
| ATAGATGAAAAGCCGCAAAA+TGG | + | 54856:14279-14298 | MS.gene59879:CDS | 35.0% | |
| CAATGACAATAACCCTCAAA+AGG | + | 54856:14149-14168 | MS.gene59879:CDS | 35.0% | |
| CTTGAAGCCAAATTTGAACA+AGG | + | 54856:13856-13875 | MS.gene59879:CDS | 35.0% | |
| GAAAGAAAGGAACTCAAAGA+AGG | + | 54856:14542-14561 | MS.gene59879:CDS | 35.0% | |
| GAGAGTTCCTTGTTCAAATT+TGG | - | 54856:13866-13885 | MS.gene59879:intergenic | 35.0% | |
| GGAAAACCAATATGACACAT+AGG | - | 54856:14856-14875 | MS.gene59879:intergenic | 35.0% | |
| TATTCCACCACCACAAAAAT+TGG | + | 54856:14014-14033 | MS.gene59879:CDS | 35.0% | |
| TGATTCTTGCTTGACTCATA+AGG | - | 54856:14594-14613 | MS.gene59879:intergenic | 35.0% | |
| TTGTGACAAGAATTGCAGAA+GGG | + | 54856:14757-14776 | MS.gene59879:CDS | 35.0% | |
| TTTGTGACAAGAATTGCAGA+AGG | + | 54856:14756-14775 | MS.gene59879:CDS | 35.0% | |
| ! | AATTCTTCTTGACCCTTTTG+AGG | - | 54856:14240-14259 | MS.gene59879:intergenic | 35.0% |
| ! | AATTCTTGTTGACCCTTTTG+AGG | - | 54856:14165-14184 | MS.gene59879:intergenic | 35.0% |
| ! | ATTCTTGTTGACCCTTTTGA+GGG | - | 54856:14164-14183 | MS.gene59879:intergenic | 35.0% |
| ! | CTCCATTTGTTAACTGGAAT+CGG | - | 54856:13787-13806 | MS.gene59879:intergenic | 35.0% |
| ! | GATTCTTCCAATTTTTGTGG+TGG | - | 54856:14024-14043 | MS.gene59879:intergenic | 35.0% |
| ! | GGCTTTTCATCTATTGATGT+TGG | - | 54856:14273-14292 | MS.gene59879:intergenic | 35.0% |
| ! | TCCAATACTTGACCATTTTG+CGG | - | 54856:14294-14313 | MS.gene59879:intergenic | 35.0% |
| !! | AATTTGCTGCTACTTCTTCT+CGG | + | 54856:14733-14752 | MS.gene59879:CDS | 35.0% |
| !! | TCAATGCTTTGAGAAGTTTG+AGG | - | 54856:14072-14091 | MS.gene59879:intergenic | 35.0% |
| !! | TTTCTGGAACAGGATATGTT+TGG | - | 54856:13819-13838 | MS.gene59879:intergenic | 35.0% |
| !!! | TTGCTTTTTGGTTGTGTCTA+TGG | - | 54856:14205-14224 | MS.gene59879:intergenic | 35.0% |
| !!! | TTGTGTTTTGGTTCTCTCTA+TGG | - | 54856:14331-14350 | MS.gene59879:intergenic | 35.0% |
| !!! | TTTTCCTTCTCTGGAACATT+TGG | - | 54856:14660-14679 | MS.gene59879:intergenic | 35.0% |
| !!! | TTTTTGTGGTGGTGGAATAT+TGG | - | 54856:14013-14032 | MS.gene59879:intergenic | 35.0% |
| AGAAAGGAACTCAAAGAAGG+AGG | + | 54856:14545-14564 | MS.gene59879:CDS | 40.0% | |
| AGAATTCCTCCCAATCAAGA+AGG | + | 54856:14693-14712 | MS.gene59879:CDS | 40.0% | |
| ATTGCAGAAGGGAAATTGAG+TGG | + | 54856:14768-14787 | MS.gene59879:CDS | 40.0% | |
| CCATTCTTCCCTTCTTGATT+GGG | - | 54856:14705-14724 | MS.gene59879:intergenic | 40.0% | |
| CTGAAGATGCTGAGAAAGAA+AGG | + | 54856:14529-14548 | MS.gene59879:CDS | 40.0% | |
| GAATTCCTCCCAATCAAGAA+GGG | + | 54856:14694-14713 | MS.gene59879:CDS | 40.0% | |
| GTTCTAGTGATTGCTGCATT+TGG | + | 54856:14828-14847 | MS.gene59879:CDS | 40.0% | |
| TCCATTCTTCCCTTCTTGAT+TGG | - | 54856:14706-14725 | MS.gene59879:intergenic | 40.0% | |
| TCCTGTTCCAGAAAATAGTG+TGG | + | 54856:13825-13844 | MS.gene59879:CDS | 40.0% | |
| TTGGAGCCTATGTGTCATAT+TGG | + | 54856:14847-14866 | MS.gene59879:CDS | 40.0% | |
| TTGGTTGTGTCTATGGATGT+TGG | - | 54856:14198-14217 | MS.gene59879:intergenic | 40.0% | |
| ! | GACCGATTCCAGTTAACAAA+TGG | + | 54856:13782-13801 | MS.gene59879:CDS | 40.0% |
| ! | TCTTCCAATTTTTGTGGTGG+TGG | - | 54856:14021-14040 | MS.gene59879:intergenic | 40.0% |
| ! | TTCTCTGCCACACTATTTTC+TGG | - | 54856:13835-13854 | MS.gene59879:intergenic | 40.0% |
| ! | TTGGTTCTCTCTATGGATGT+TGG | - | 54856:14324-14343 | MS.gene59879:intergenic | 40.0% |
| !!! | GTGTCTATGGATGTTGGTTT+TGG | - | 54856:14192-14211 | MS.gene59879:intergenic | 40.0% |
| !!! | TTGTGTTTTGGTTCTCTCTG+TGG | - | 54856:14406-14425 | MS.gene59879:intergenic | 40.0% |
| AAAGGCTCAAAAGGGTGAAG+AGG | + | 54856:14434-14453 | MS.gene59879:CDS | 45.0% | |
| CCCAATCAAGAAGGGAAGAA+TGG | + | 54856:14702-14721 | MS.gene59879:CDS | 45.0% | |
| GCCGCAAAATGGTCAAGTAT+TGG | + | 54856:14290-14309 | MS.gene59879:CDS | 45.0% | |
| GGACATGTCACGAAAGTTGA+GGG | + | 54856:13747-13766 | MS.gene59879:CDS | 45.0% | |
| TCACGAAAGTTGAGGGTTAC+AGG | + | 54856:13754-13773 | MS.gene59879:CDS | 45.0% | |
| TGCTCCAAATGTTCCAGAGA+AGG | + | 54856:14653-14672 | MS.gene59879:CDS | 45.0% | |
| TGGACATGTCACGAAAGTTG+AGG | + | 54856:13746-13765 | MS.gene59879:CDS | 45.0% | |
| TTCTTCCCTTCTTGATTGGG+AGG | - | 54856:14702-14721 | MS.gene59879:intergenic | 45.0% | |
| ! | CAAGCTTCCTTTTCCTTCTC+TGG | - | 54856:14669-14688 | MS.gene59879:intergenic | 45.0% |
| ! | TTGGTTCTCTCTGTGGATGT+TGG | - | 54856:14399-14418 | MS.gene59879:intergenic | 45.0% |
| !!! | GCCACACTATTTTCTGGAAC+AGG | - | 54856:13829-13848 | MS.gene59879:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 54856 | gene | 13730 | 14881 | 13730 | ID=MS.gene59879 |
| 54856 | mRNA | 13730 | 14881 | 13730 | ID=MS.gene59879.t1;Parent=MS.gene59879 |
| 54856 | exon | 13730 | 14881 | 13730 | ID=MS.gene59879.t1.exon1;Parent=MS.gene59879.t1 |
| 54856 | CDS | 13730 | 14881 | 13730 | ID=cds.MS.gene59879.t1;Parent=MS.gene59879.t1 |
| Gene Sequence |
| Protein sequence |
