Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59941.t1 | RHN74919.1 | 98.7 | 374 | 5 | 0 | 1 | 374 | 1 | 374 | 4.60E-196 | 693.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59941.t1 | F4KGE8 | 24.3 | 329 | 216 | 4 | 70 | 373 | 161 | 481 | 2.1e-24 | 114.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59941.t1 | G7ITF5 | 98.7 | 374 | 5 | 0 | 1 | 374 | 4 | 377 | 3.3e-196 | 693.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058720 | MS.gene59941 | 0.803097 | 4.10E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene59941 | MS.gene058492 | PPI |
| MS.gene59941 | MS.gene48940 | PPI |
| MS.gene59941 | MS.gene45832 | PPI |
| MS.gene59941 | MS.gene48223 | PPI |
| MS.gene59941 | MS.gene023604 | PPI |
| MS.gene59941 | MS.gene059205 | PPI |
| MS.gene59941 | MS.gene27831 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59941.t1 | MTR_2g075910 | 98.663 | 374 | 5 | 0 | 1 | 374 | 4 | 377 | 0.0 | 763 |
| MS.gene59941.t1 | MTR_8g059485 | 57.895 | 380 | 136 | 9 | 1 | 374 | 4 | 365 | 1.16e-137 | 400 |
| MS.gene59941.t1 | MTR_8g059485 | 57.895 | 380 | 136 | 9 | 1 | 374 | 13 | 374 | 1.42e-137 | 400 |
| MS.gene59941.t1 | MTR_8g087400 | 43.127 | 371 | 202 | 4 | 9 | 374 | 14 | 380 | 5.82e-98 | 299 |
| MS.gene59941.t1 | MTR_4g025420 | 40.256 | 313 | 175 | 4 | 68 | 373 | 34 | 341 | 2.76e-73 | 234 |
| MS.gene59941.t1 | MTR_0178s0030 | 29.467 | 319 | 207 | 4 | 66 | 372 | 139 | 451 | 7.28e-38 | 143 |
| MS.gene59941.t1 | MTR_5g075180 | 31.210 | 314 | 188 | 5 | 61 | 373 | 78 | 364 | 8.23e-35 | 133 |
| MS.gene59941.t1 | MTR_3g462150 | 29.193 | 322 | 203 | 8 | 70 | 373 | 92 | 406 | 2.36e-34 | 132 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59941.t1 | AT1G53380 | 50.000 | 380 | 177 | 6 | 1 | 374 | 4 | 376 | 4.45e-129 | 378 |
| MS.gene59941.t1 | AT1G53380 | 50.000 | 380 | 177 | 6 | 1 | 374 | 4 | 376 | 4.45e-129 | 378 |
| MS.gene59941.t1 | AT1G53380 | 50.000 | 380 | 177 | 6 | 1 | 374 | 4 | 376 | 4.45e-129 | 378 |
| MS.gene59941.t1 | AT1G53380 | 50.000 | 380 | 177 | 6 | 1 | 374 | 4 | 376 | 4.45e-129 | 378 |
| MS.gene59941.t1 | AT1G53380 | 50.000 | 380 | 177 | 6 | 1 | 374 | 4 | 376 | 4.45e-129 | 378 |
| MS.gene59941.t1 | AT3G14870 | 47.804 | 387 | 184 | 5 | 1 | 374 | 4 | 385 | 6.99e-127 | 373 |
| MS.gene59941.t1 | AT3G14870 | 47.804 | 387 | 184 | 5 | 1 | 374 | 4 | 385 | 6.99e-127 | 373 |
| MS.gene59941.t1 | AT3G14870 | 47.804 | 387 | 184 | 5 | 1 | 374 | 4 | 385 | 6.99e-127 | 373 |
| MS.gene59941.t1 | AT3G14870 | 47.804 | 387 | 184 | 5 | 1 | 374 | 5 | 386 | 8.04e-127 | 373 |
| MS.gene59941.t1 | AT3G14870 | 47.804 | 387 | 184 | 5 | 1 | 374 | 8 | 389 | 8.47e-127 | 373 |
| MS.gene59941.t1 | AT1G29300 | 40.153 | 391 | 199 | 6 | 2 | 374 | 6 | 379 | 2.65e-81 | 256 |
| MS.gene59941.t1 | AT2G45260 | 39.130 | 322 | 189 | 2 | 59 | 373 | 26 | 347 | 1.14e-75 | 240 |
| MS.gene59941.t1 | AT4G34080 | 37.500 | 280 | 168 | 2 | 59 | 331 | 2 | 281 | 3.63e-57 | 188 |
| MS.gene59941.t1 | AT4G33320 | 35.664 | 286 | 176 | 3 | 59 | 336 | 2 | 287 | 8.40e-51 | 172 |
| MS.gene59941.t1 | AT2G30380 | 30.565 | 301 | 173 | 2 | 73 | 373 | 76 | 340 | 6.12e-36 | 135 |
| MS.gene59941.t1 | AT3G60680 | 28.013 | 307 | 208 | 3 | 71 | 372 | 120 | 418 | 1.48e-35 | 136 |
| MS.gene59941.t1 | AT5G58960 | 26.506 | 332 | 205 | 6 | 70 | 373 | 86 | 406 | 1.26e-30 | 122 |
| MS.gene59941.t1 | AT5G58960 | 26.506 | 332 | 205 | 6 | 70 | 373 | 86 | 406 | 1.26e-30 | 122 |
| MS.gene59941.t1 | AT5G58960 | 27.607 | 326 | 209 | 7 | 70 | 373 | 161 | 481 | 2.02e-30 | 122 |
| MS.gene59941.t1 | AT2G32130 | 44.355 | 124 | 69 | 0 | 70 | 193 | 34 | 157 | 6.07e-27 | 105 |
| MS.gene59941.t1 | AT4G36100 | 46.078 | 102 | 52 | 1 | 59 | 157 | 2 | 103 | 9.35e-21 | 90.5 |
Find 0 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAGAAAAAATCAACAAAAT+TGG | + | 14734:7745-7764 | MS.gene59941:CDS | 15.0% |
| ! | GTTTCTTAAAGAGAAATTAG+TGG | + | 14734:7380-7399 | MS.gene59941:CDS | 25.0% |
| ! | TATATCTGATACAAATCCAA+AGG | + | 14734:6960-6979 | MS.gene59941:CDS | 25.0% |
| ! | TCATTTGGAAGATTGAAATT+AGG | - | 14734:7711-7730 | MS.gene59941:intergenic | 25.0% |
| !! | AAAAATGATGACTCCATTTT+TGG | - | 14734:7885-7904 | MS.gene59941:intergenic | 25.0% |
| !!! | AAAATGGAGTCATCATTTTT+TGG | + | 14734:7885-7904 | MS.gene59941:CDS | 25.0% |
| !!! | GATGCAAAAAAATCACTTTT+TGG | - | 14734:7951-7970 | MS.gene59941:intergenic | 25.0% |
| !!! | TGTTGATTTTTTCTATCATC+TGG | - | 14734:7741-7760 | MS.gene59941:intergenic | 25.0% |
| ATGAAATGAGATCTGCAAAA+TGG | + | 14734:7568-7587 | MS.gene59941:CDS | 30.0% | |
| GATAATGTTGTTCACATATC+AGG | + | 14734:7468-7487 | MS.gene59941:CDS | 30.0% | |
| TGAAATGAGATCTGCAAAAT+GGG | + | 14734:7569-7588 | MS.gene59941:CDS | 30.0% | |
| TGGAAAAGACTACAATAACT+TGG | + | 14734:6995-7014 | MS.gene59941:CDS | 30.0% | |
| ! | GATTTTTGCTAGCATTTCAA+CGG | + | 14734:7095-7114 | MS.gene59941:CDS | 30.0% |
| ! | TTTTGCATCTTTTGCTGAAA+TGG | + | 14734:7962-7981 | MS.gene59941:CDS | 30.0% |
| GAGAAGAGATTGAATCAAAG+TGG | + | 14734:7429-7448 | MS.gene59941:CDS | 35.0% | |
| TCCAAAGGATGAAACAAACA+TGG | + | 14734:6975-6994 | MS.gene59941:CDS | 35.0% | |
| TCCATGTTTGTTTCATCCTT+TGG | - | 14734:6979-6998 | MS.gene59941:intergenic | 35.0% | |
| TGATGCTATTGAACACAATG+TGG | + | 14734:7608-7627 | MS.gene59941:CDS | 35.0% | |
| TTCCTAGCTAATTTCTTGCT+TGG | - | 14734:6895-6914 | MS.gene59941:intergenic | 35.0% | |
| ! | GAGACTTGTTCATCCAAAAA+TGG | + | 14734:7869-7888 | MS.gene59941:CDS | 35.0% |
| !! | TGCTCTAAGATGAAGAACTT+TGG | - | 14734:6923-6942 | MS.gene59941:intergenic | 35.0% |
| !! | TTTTTGGCAACATGATTCAC+AGG | + | 14734:7901-7920 | MS.gene59941:CDS | 35.0% |
| AATTGCGCAAACGATGATCT+TGG | - | 14734:7831-7850 | None:intergenic | 40.0% | |
| AGAACAGTAGCAAAATGGCT+AGG | - | 14734:7501-7520 | None:intergenic | 40.0% | |
| AGCTGAATCGAAGGAGATAA+AGG | + | 14734:7284-7303 | MS.gene59941:CDS | 40.0% | |
| CACCAAGCAAGAAATTAGCT+AGG | + | 14734:6890-6909 | MS.gene59941:CDS | 40.0% | |
| CAGAAGCTTCAATTCCATCA+GGG | - | 14734:7167-7186 | MS.gene59941:intergenic | 40.0% | |
| GACGAAGAACAGTAGCAAAA+TGG | - | 14734:7506-7525 | MS.gene59941:intergenic | 40.0% | |
| GCTAGCATTTCAACGGTTAA+AGG | + | 14734:7102-7121 | MS.gene59941:CDS | 40.0% | |
| GCTGAATCGAAGGAGATAAA+GGG | + | 14734:7285-7304 | MS.gene59941:CDS | 40.0% | |
| TCAATTCCATCAGGGTCAAA+AGG | - | 14734:7159-7178 | MS.gene59941:intergenic | 40.0% | |
| TCAGAAGCTTCAATTCCATC+AGG | - | 14734:7168-7187 | MS.gene59941:intergenic | 40.0% | |
| TCATCTGGAAGTGACTCATT+TGG | - | 14734:7726-7745 | MS.gene59941:intergenic | 40.0% | |
| ! | ATTCACAGGAACCTTTTGAG+TGG | + | 14734:7915-7934 | MS.gene59941:CDS | 40.0% |
| ! | CTTTTGCTGAAATGGCTAAG+AGG | + | 14734:7970-7989 | MS.gene59941:CDS | 40.0% |
| !! | GATCGATTCGAAACTTTGTG+AGG | + | 14734:7535-7554 | MS.gene59941:CDS | 40.0% |
| AGTATCGCTTTCTCTGGTGA+AGG | - | 14734:7261-7280 | MS.gene59941:intergenic | 45.0% | |
| CTGTGATTCCAACTTCTTCG+CGG | - | 14734:7334-7353 | MS.gene59941:intergenic | 45.0% | |
| GAAACTCTCCACCACTCAAA+AGG | - | 14734:7929-7948 | MS.gene59941:intergenic | 45.0% | |
| ! | GGGTGTCATAAAAACCTACG+AGG | + | 14734:7305-7324 | MS.gene59941:CDS | 45.0% |
| CATCGTTTGCGCAATTCTGC+AGG | + | 14734:7835-7854 | MS.gene59941:CDS | 50.0% | |
| CGCGAAGAAGTTGGAATCAC+AGG | + | 14734:7332-7351 | MS.gene59941:CDS | 50.0% | |
| GATACTCGCAGCTGAATCGA+AGG | + | 14734:7275-7294 | MS.gene59941:CDS | 50.0% | |
| TGCTACTGTTCTTCGTCACG+CGG | + | 14734:7509-7528 | MS.gene59941:CDS | 50.0% | |
| ! | CAGACACCTTTTGACCCTGA+TGG | + | 14734:7150-7169 | MS.gene59941:CDS | 50.0% |
| !! | CACAGGAACCTTTTGAGTGG+TGG | + | 14734:7918-7937 | MS.gene59941:CDS | 50.0% |
| !! | TTGCTTGGTGTCACTGCTGA+AGG | - | 14734:6880-6899 | MS.gene59941:intergenic | 50.0% |
| CGAGGTTACCGCGAAGAAGT+TGG | + | 14734:7323-7342 | MS.gene59941:CDS | 55.0% | |
| CTTCTTCGCGGTAACCTCGT+AGG | - | 14734:7322-7341 | MS.gene59941:intergenic | 55.0% | |
| ! | GCTGCGAGTATCGCTTTCTC+TGG | - | 14734:7267-7286 | MS.gene59941:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 14734 | gene | 6871 | 7992 | 6871 | ID=MS.gene59941 |
| 14734 | mRNA | 6871 | 7992 | 6871 | ID=MS.gene59941.t1;Parent=MS.gene59941 |
| 14734 | exon | 6871 | 7992 | 6871 | ID=MS.gene59941.t1.exon1;Parent=MS.gene59941.t1 |
| 14734 | CDS | 6871 | 7992 | 6871 | ID=cds.MS.gene59941.t1;Parent=MS.gene59941.t1 |
| Gene Sequence |
| Protein sequence |
