AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60222

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60222.t1 MTR_4g010830 84.570 1011 115 5 1 1001 1 980 0.0 1670
MS.gene60222.t1 MTR_5g013420 73.499 1049 203 13 13 1001 56 1089 0.0 1481
MS.gene60222.t1 MTR_5g013420 73.403 1049 204 13 13 1001 56 1089 0.0 1476
MS.gene60222.t1 MTR_4g010550 85.253 495 37 6 512 1001 1 464 0.0 821
MS.gene60222.t1 MTR_7g445710 42.135 534 221 11 489 1001 77 543 4.54e-121 381
MS.gene60222.t1 MTR_8g042750 40.105 571 313 14 450 998 122 685 1.02e-120 385
MS.gene60222.t1 MTR_3g011440 40.367 545 293 15 480 1000 127 663 2.68e-118 377
MS.gene60222.t1 MTR_7g084090 41.154 520 264 14 516 1001 246 757 1.30e-116 376
MS.gene60222.t1 MTR_7g088370 40.684 526 271 18 493 1000 202 704 2.11e-115 371
MS.gene60222.t1 MTR_8g070070 43.167 461 238 12 530 970 299 755 9.91e-115 371
MS.gene60222.t1 MTR_6g061270 40.996 522 265 13 516 1001 180 694 1.03e-114 369
MS.gene60222.t1 MTR_7g450730 41.065 526 215 10 483 1001 68 505 1.23e-113 360
MS.gene60222.t1 MTR_8g070075 42.082 461 247 9 528 970 290 748 1.52e-113 368
MS.gene60222.t1 MTR_8g071030 42.266 459 243 10 531 970 280 735 1.19e-107 352
MS.gene60222.t1 MTR_1g035420 37.522 557 300 14 454 975 106 649 1.91e-107 348
MS.gene60222.t1 MTR_6g061200 40.808 495 249 12 527 1001 195 665 2.54e-107 348
MS.gene60222.t1 MTR_7g445530 41.153 503 208 11 490 971 78 513 8.74e-107 342
MS.gene60222.t1 MTR_1g048950 40.037 537 260 12 492 1001 32 533 5.38e-103 333
MS.gene60222.t1 MTR_6g044690 38.758 467 249 13 528 970 109 562 4.15e-92 305
MS.gene60222.t1 MTR_0376s0030 35.867 513 279 15 494 970 62 560 4.18e-87 291
MS.gene60222.t1 MTR_1g035400 36.523 512 284 16 494 975 68 568 1.25e-84 285
MS.gene60222.t1 MTR_6g043960 35.608 469 236 14 530 970 50 480 3.62e-76 259
MS.gene60222.t1 MTR_7g084090 40.310 387 206 11 516 880 246 629 4.26e-76 263
MS.gene60222.t1 MTR_2g054460 33.002 503 262 11 494 970 9 462 3.39e-68 236
MS.gene60222.t1 MTR_7g052480 40.881 318 168 7 698 1001 245 556 2.15e-66 233
MS.gene60222.t1 MTR_5g018850 35.802 324 143 10 719 1001 1208 1507 1.88e-44 176
MS.gene60222.t1 MTR_8g078490 40.724 221 102 7 787 1001 1056 1253 8.44e-42 167
MS.gene60222.t1 MTR_7g445600 48.750 160 40 2 812 971 239 356 9.94e-42 157
MS.gene60222.t1 MTR_7g445600 37.278 169 89 3 490 658 74 225 3.98e-24 105
MS.gene60222.t1 MTR_4g132610 39.631 217 108 4 788 1001 851 1047 7.64e-39 157
MS.gene60222.t1 MTR_8g078505 38.426 216 103 6 786 996 343 533 7.44e-36 144
MS.gene60222.t1 MTR_1g069570 31.024 332 155 13 721 996 428 741 2.77e-30 129
MS.gene60222.t1 MTR_1g080340 29.970 337 159 11 721 999 267 584 7.69e-30 126
MS.gene60222.t1 MTR_7g098390 25.872 344 167 9 715 996 380 697 2.98e-25 112
MS.gene60222.t1 MTR_7g445810 36.364 198 97 6 490 679 91 267 7.40e-25 105
MS.gene60222.t1 MTR_1g069570 29.924 264 115 11 721 929 428 676 5.83e-21 99.4
MS.gene60222.t1 MTR_0071s0100 34.021 194 92 6 807 1000 191 348 7.54e-19 90.5
MS.gene60222.t1 MTR_6g059310 31.313 198 102 6 803 1000 87 250 1.53e-18 88.6
MS.gene60222.t1 MTR_1g098370 32.821 195 93 8 806 997 75 234 1.72e-18 90.5
MS.gene60222.t1 MTR_6g059310 31.313 198 102 6 803 1000 87 250 2.14e-18 88.6
MS.gene60222.t1 MTR_3g088625 34.184 196 85 7 811 1000 1201 1358 2.55e-18 91.7
MS.gene60222.t1 MTR_3g088625 34.184 196 85 7 811 1000 1307 1464 2.65e-18 91.7
MS.gene60222.t1 MTR_1g098000 32.308 195 94 8 806 997 72 231 4.55e-18 89.0
MS.gene60222.t1 MTR_5g098680 34.615 156 85 4 556 700 267 416 3.04e-16 84.0
MS.gene60222.t1 MTR_8g102580 33.974 156 86 4 556 700 261 410 1.90e-15 81.6
MS.gene60222.t1 MTR_6g027690 29.046 241 127 9 766 1000 217 419 6.44e-15 78.6
MS.gene60222.t1 MTR_8g027725 33.548 155 65 5 827 975 791 913 9.73e-13 73.2
MS.gene60222.t1 MTR_8g027725 33.548 155 65 5 827 975 806 928 1.10e-12 72.8
MS.gene60222.t1 MTR_1g064770 45.312 64 34 1 800 862 27 90 1.48e-12 64.7
MS.gene60222.t1 MTR_7g117355 33.548 155 65 5 827 975 821 943 1.92e-12 72.0
MS.gene60222.t1 MTR_1g012910 27.485 171 96 5 830 1000 528 670 8.02e-12 69.7
MS.gene60222.t1 MTR_1g012910 27.485 171 96 5 830 1000 365 507 1.57e-11 68.6
MS.gene60222.t1 MTR_6g044000 48.980 49 25 0 922 970 119 167 3.17e-11 63.5
MS.gene60222.t1 MTR_2g036060 26.667 165 87 4 829 993 1033 1163 5.23e-11 67.4
MS.gene60222.t1 MTR_2g036060 26.667 165 87 4 829 993 1058 1188 5.45e-11 67.4
MS.gene60222.t1 MTR_1g008230 32.867 143 58 4 839 975 903 1013 7.21e-11 67.0
MS.gene60222.t1 MTR_1g008230 32.867 143 58 4 839 975 625 735 7.24e-11 66.6
MS.gene60222.t1 MTR_3g091310 34.266 143 56 5 839 975 838 948 8.93e-11 66.6
MS.gene60222.t1 MTR_3g091310 34.266 143 56 5 839 975 932 1042 9.31e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60222.t1 AT2G22740 61.950 523 182 8 494 1001 270 790 0.0 642
MS.gene60222.t1 AT2G22740 61.950 523 182 8 494 1001 270 790 0.0 642
MS.gene60222.t1 AT2G35160 60.078 516 172 5 493 1001 270 758 0.0 637
MS.gene60222.t1 AT2G35160 60.078 516 172 5 493 1001 306 794 0.0 637
MS.gene60222.t1 AT2G35160 60.078 516 172 5 493 1001 306 794 0.0 637
MS.gene60222.t1 AT2G35160 60.078 516 172 5 493 1001 306 794 0.0 637
MS.gene60222.t1 AT2G35160 60.078 516 172 5 493 1001 306 794 0.0 637
MS.gene60222.t1 AT5G04940 41.418 536 284 16 483 1000 146 669 3.30e-120 383
MS.gene60222.t1 AT5G04940 41.418 536 284 16 483 1000 146 669 3.30e-120 383
MS.gene60222.t1 AT5G13960 40.187 535 277 14 496 1001 104 624 4.50e-114 365
MS.gene60222.t1 AT5G13960 42.412 481 245 12 550 1001 13 490 1.64e-112 357
MS.gene60222.t1 AT1G73100 40.187 535 290 15 482 1000 148 668 8.42e-111 358
MS.gene60222.t1 AT4G13460 38.627 510 288 11 486 975 137 641 1.40e-104 341
MS.gene60222.t1 AT4G13460 38.627 510 288 11 486 975 137 641 1.40e-104 341
MS.gene60222.t1 AT2G33290 35.443 553 326 11 442 970 92 637 2.79e-98 324
MS.gene60222.t1 AT2G33290 35.443 553 326 11 442 970 92 637 2.79e-98 324
MS.gene60222.t1 AT2G33290 35.443 553 326 11 442 970 92 637 2.79e-98 324
MS.gene60222.t1 AT2G33290 35.443 553 326 11 442 970 92 637 2.79e-98 324
MS.gene60222.t1 AT2G24740 35.219 548 279 17 482 998 250 752 6.66e-86 293
MS.gene60222.t1 AT1G17770 36.998 473 269 12 551 1000 226 692 1.05e-85 291
MS.gene60222.t1 AT5G47150 46.977 215 111 3 490 702 114 327 1.19e-50 182
MS.gene60222.t1 AT5G47160 41.636 269 149 5 430 695 144 407 1.11e-45 170
MS.gene60222.t1 AT5G47160 41.636 269 149 5 430 695 144 407 1.11e-45 170
MS.gene60222.t1 AT5G47160 41.636 269 149 5 430 695 144 407 1.11e-45 170
MS.gene60222.t1 AT2G23740 33.956 321 160 11 711 1000 1075 1374 6.37e-38 155
MS.gene60222.t1 AT2G23740 33.956 321 160 11 711 1000 1082 1381 7.22e-38 154
MS.gene60222.t1 AT2G23740 33.956 321 160 11 711 1000 1082 1381 7.22e-38 154
MS.gene60222.t1 AT2G23740 33.956 321 160 11 711 1000 1082 1381 7.22e-38 154
MS.gene60222.t1 AT3G04380 28.453 362 183 13 692 999 121 460 2.70e-29 123
MS.gene60222.t1 AT3G04380 28.453 362 183 13 692 999 94 433 2.93e-29 123
MS.gene60222.t1 AT3G04380 28.453 362 183 13 692 999 105 444 3.93e-29 122
MS.gene60222.t1 AT3G04380 28.453 362 183 13 692 999 121 460 4.51e-29 122
MS.gene60222.t1 AT3G04380 28.453 362 183 13 692 999 94 433 5.21e-29 122
MS.gene60222.t1 AT3G04380 28.453 362 183 13 692 999 78 417 6.25e-29 121
MS.gene60222.t1 AT2G05900 37.037 216 120 9 569 772 1 212 1.79e-28 117
MS.gene60222.t1 AT2G05900 41.463 82 40 2 917 998 236 309 2.46e-11 66.6
MS.gene60222.t1 AT2G23740 34.000 250 117 9 711 929 1082 1314 1.49e-27 121
MS.gene60222.t1 AT2G23740 34.000 250 117 9 711 929 1082 1314 1.49e-27 121
MS.gene60222.t1 AT5G43990 28.443 334 162 13 721 999 373 684 3.55e-26 115
MS.gene60222.t1 AT5G43990 28.443 334 162 13 721 999 373 684 3.55e-26 115
MS.gene60222.t1 AT5G43990 28.443 334 162 13 721 999 373 684 3.55e-26 115
MS.gene60222.t1 AT5G43990 28.443 334 162 13 721 999 373 684 3.55e-26 115
MS.gene60222.t1 AT5G43990 28.443 334 162 13 721 999 396 707 3.85e-26 115
MS.gene60222.t1 AT5G43990 28.443 334 162 13 721 999 396 707 3.85e-26 115
MS.gene60222.t1 AT5G43990 28.443 334 162 13 721 999 393 704 4.06e-26 115
MS.gene60222.t1 AT5G43990 28.443 334 162 13 721 999 393 704 4.06e-26 115
MS.gene60222.t1 AT5G43990 28.443 334 162 13 721 999 393 704 4.06e-26 115
MS.gene60222.t1 AT5G43990 28.443 334 162 13 721 999 393 704 4.06e-26 115
MS.gene60222.t1 AT5G43990 28.443 334 162 13 721 999 416 727 4.69e-26 115
MS.gene60222.t1 AT5G43990 28.443 334 162 13 721 999 416 727 4.69e-26 115
MS.gene60222.t1 AT5G43990 28.443 334 162 13 721 999 304 615 7.07e-26 114
MS.gene60222.t1 AT1G04050 33.668 199 110 5 804 999 542 721 9.16e-26 114
MS.gene60222.t1 AT1G04050 33.668 199 110 5 804 999 542 721 9.16e-26 114
MS.gene60222.t1 AT1G04050 33.668 199 110 5 804 999 438 617 9.29e-26 114
MS.gene60222.t1 AT5G43990 28.443 334 162 13 721 999 324 635 9.32e-26 114
MS.gene60222.t1 AT5G43990 28.443 334 162 13 721 999 324 635 9.32e-26 114
MS.gene60222.t1 AT5G43990 28.443 334 162 13 721 999 324 635 9.32e-26 114
MS.gene60222.t1 AT5G43990 28.443 334 162 13 721 999 324 635 9.32e-26 114
MS.gene60222.t1 AT3G03750 33.163 196 115 6 806 1000 169 349 9.87e-21 95.5
MS.gene60222.t1 AT4G30860 30.034 293 136 13 717 1000 232 464 6.40e-19 91.7
MS.gene60222.t1 AT1G76710 32.828 198 95 7 806 1000 66 228 7.76e-19 91.7
MS.gene60222.t1 AT1G76710 32.828 198 95 7 806 1000 66 228 7.76e-19 91.7
MS.gene60222.t1 AT1G76710 32.828 198 95 7 806 1000 66 228 9.58e-19 91.3
MS.gene60222.t1 AT1G76710 32.828 198 95 7 806 1000 66 228 1.17e-18 91.3
MS.gene60222.t1 AT1G77300 31.579 190 96 8 813 1000 1009 1166 7.29e-17 87.0
MS.gene60222.t1 AT1G77300 31.579 190 96 8 813 1000 1009 1166 7.78e-17 86.7
MS.gene60222.t1 AT1G57820 33.758 157 86 5 556 700 277 427 2.74e-16 84.3
MS.gene60222.t1 AT1G57820 33.758 157 86 5 556 700 277 427 2.74e-16 84.3
MS.gene60222.t1 AT1G57820 33.117 154 88 5 556 700 277 424 1.93e-15 81.3
MS.gene60222.t1 AT3G59960 30.058 173 85 6 829 1000 112 249 2.84e-14 75.1
MS.gene60222.t1 AT3G59960 30.058 173 85 6 829 1000 112 249 2.84e-14 75.1
MS.gene60222.t1 AT3G59960 30.058 173 85 6 829 1000 112 249 2.84e-14 75.1
MS.gene60222.t1 AT3G59960 30.058 173 85 6 829 1000 112 249 8.46e-14 74.3
MS.gene60222.t1 AT4G08590 36.641 131 65 4 556 679 237 356 1.23e-13 75.1
MS.gene60222.t1 AT2G44150 29.651 172 87 6 829 1000 117 254 1.60e-13 73.9
MS.gene60222.t1 AT3G61740 34.615 156 63 6 827 975 838 961 6.15e-13 73.9
MS.gene60222.t1 AT3G61740 34.615 156 63 6 827 975 874 997 7.60e-13 73.6
MS.gene60222.t1 AT4G08590 35.878 131 67 4 556 679 237 357 1.57e-12 71.2
MS.gene60222.t1 AT4G08590 35.878 131 67 4 556 679 237 357 1.65e-12 70.9
MS.gene60222.t1 AT4G08590 35.878 131 67 4 556 679 237 357 1.65e-12 70.9
MS.gene60222.t1 AT3G03750 29.082 196 107 8 806 1000 169 333 1.65e-12 70.5
MS.gene60222.t1 AT4G08590 35.878 131 67 4 556 679 237 357 1.97e-12 70.9
MS.gene60222.t1 AT4G27910 31.707 164 69 6 839 996 896 1022 7.33e-12 70.5
MS.gene60222.t1 AT1G02580 28.571 224 125 6 766 987 485 675 2.77e-11 68.2
MS.gene60222.t1 AT5G53430 31.707 164 69 6 839 996 912 1038 4.78e-11 67.8

Find 215 sgRNAs with CRISPR-Local

Find 263 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGCTCGAACTATGTGATTT+TGG 0.106914 4.3:+85932967 None:intergenic
TCCTGAAGGGATGGAATTTA+AGG 0.160993 4.3:+85931835 None:intergenic
CGAAGACGATGTGCCTATTT+TGG 0.176590 4.3:-85933952 MS.gene60222:CDS
TTGAGTCAGAATCTGGAAAA+AGG 0.241271 4.3:+85931681 None:intergenic
AGAATAGAGCTTAATATAAT+TGG 0.247368 4.3:-85932670 MS.gene60222:CDS
TGGCCTTCATCGCCAGATTC+AGG 0.250113 4.3:-85932650 MS.gene60222:CDS
ATATCCCCATTATGATTAAA+TGG 0.280457 4.3:+85931998 None:intergenic
GTTGAAGGGCACTTGCATTT+AGG 0.290171 4.3:+85931941 None:intergenic
TGCAACTAGTATTGTTTCTT+CGG 0.298661 4.3:-85932590 MS.gene60222:CDS
CTTGAACTTGAGTCAGAATC+TGG 0.299589 4.3:+85931674 None:intergenic
AAAGGTGTACTGAATCATTC+TGG 0.301672 4.3:-85933108 MS.gene60222:CDS
GAGATTCCATTTAATCATAA+TGG 0.305009 4.3:-85932004 MS.gene60222:CDS
AGATTCCATTTAATCATAAT+GGG 0.307894 4.3:-85932003 MS.gene60222:CDS
TGAAGACTGTACCTTGAAAA+AGG 0.316856 4.3:-85934066 MS.gene60222:CDS
AAACGATGAACCTGCAAAAC+TGG 0.322145 4.3:-85933835 MS.gene60222:CDS
GCAACTAGTATTGTTTCTTC+GGG 0.325203 4.3:-85932589 MS.gene60222:CDS
CATTCTGAGCATAAAGATTA+GGG 0.326248 4.3:+85931570 None:intergenic
AAAGGATGTAATTGTACTAA+TGG 0.326952 4.3:-85932073 MS.gene60222:CDS
TTAGTACAATTACATCCTTT+TGG 0.326965 4.3:+85932076 None:intergenic
ACGTCAAAAGTCTCCAAAAT+AGG 0.328102 4.3:+85933939 None:intergenic
GTACGCAACAACATTTCTTA+TGG 0.328564 4.3:+85932041 None:intergenic
GATAAGAGTGGCAAAGATTC+AGG 0.330173 4.3:-85934305 MS.gene60222:CDS
CAAGTTGGCTAACTCTATTA+TGG 0.333516 4.3:+85931915 None:intergenic
GGAACCACCAATATTGATAT+TGG 0.341978 4.3:+85933677 None:intergenic
TGAAGGAGGCTTTAACTAAA+AGG 0.341984 4.3:-85933644 MS.gene60222:CDS
GAGTCATGTTGGCAGGATAT+AGG 0.343797 4.3:-85932343 MS.gene60222:CDS
GTCTTATCTGATCAAGTAGA+TGG 0.349850 4.3:-85934164 MS.gene60222:CDS
TGAGGTTAACATAAATTCTA+AGG 0.349922 4.3:-85933391 MS.gene60222:CDS
GGGATAAGATTGCAACATTC+AGG 0.352004 4.3:+85932100 None:intergenic
AATATCTTGGGATCTGTTCC+AGG 0.356395 4.3:-85932721 MS.gene60222:CDS
TCCTTAAATTCCATCCCTTC+AGG 0.359885 4.3:-85931836 MS.gene60222:CDS
ATACGATGCCTTTGATATAT+AGG 0.362188 4.3:+85933746 None:intergenic
AATCTATACCACCCTGAATC+TGG 0.363176 4.3:+85932638 None:intergenic
AAAGATTAGGGGAGCAACTA+TGG 0.371994 4.3:+85931582 None:intergenic
TCAAGTAGATGGGGATAGTT+TGG 0.377351 4.3:-85934153 MS.gene60222:CDS
TACACCCCATCCCATTGAAT+TGG 0.382244 4.3:+85931862 None:intergenic
TCGTTGTCCAATATCAATAT+TGG 0.394388 4.3:-85933684 MS.gene60222:CDS
ATTGGTTGGCATGGAAACTA+TGG 0.396042 4.3:-85934105 MS.gene60222:CDS
ACATTCTGAGCATAAAGATT+AGG 0.400673 4.3:+85931569 None:intergenic
GACATAGCTGCCTCGCCAGC+TGG 0.412521 4.3:+85933723 None:intergenic
ACTTCTTTCATATTTGACTT+TGG 0.416957 4.3:+85932811 None:intergenic
ACTTCCTTCAAGCCAAGCTC+TGG 0.417747 4.3:+85932268 None:intergenic
TCGGTTATGTATTTAAATGT+AGG 0.420707 4.3:+85932133 None:intergenic
TGTGGTTCTGTGGAATGTAC+TGG 0.421622 4.3:-85931395 MS.gene60222:CDS
AGTCATGTTGGCAGGATATA+GGG 0.423970 4.3:-85932342 MS.gene60222:CDS
CGGTTATGTATTTAAATGTA+GGG 0.424018 4.3:+85932134 None:intergenic
CACCCTGAATCTGGCGATGA+AGG 0.425090 4.3:+85932647 None:intergenic
ACTATCGATGCCGCTCAGTT+TGG 0.425253 4.3:-85931626 MS.gene60222:CDS
TTCTATGAAAAGCATGGAAC+AGG 0.428026 4.3:+85932860 None:intergenic
TCAACCAGAGCTTGGCTTGA+AGG 0.433037 4.3:-85932272 MS.gene60222:CDS
TGGCGAGGTTCTTGAAGAAA+AGG 0.434427 4.3:-85931792 MS.gene60222:CDS
TAATGAAGGGGAGAATATCT+TGG 0.437477 4.3:-85932734 MS.gene60222:CDS
TAAAACCAATTCAATGGGAT+GGG 0.437648 4.3:-85931867 MS.gene60222:CDS
GAAAGTAGTTCAAGGTTTGA+TGG 0.438952 4.3:-85933301 MS.gene60222:CDS
TCAAAGAAGAAAGCTCCTTT+CGG 0.441008 4.3:+85933535 None:intergenic
CATAATAGAGTTAGCCAACT+TGG 0.449808 4.3:-85931914 MS.gene60222:CDS
GAAAAGATACTCATCATTAC+CGG 0.451653 4.3:+85931754 None:intergenic
TTGATAATGTCCAGCTGGAC+AGG 0.452841 4.3:-85933152 MS.gene60222:CDS
TGTGTCGAAGATATTTCCTA+TGG 0.454904 4.3:-85932208 MS.gene60222:CDS
TGCTGTCTTGATGATCATAA+AGG 0.455362 4.3:+85931546 None:intergenic
ACGGAACGAAAAGGAAAGAA+AGG 0.455459 4.3:-85933184 MS.gene60222:CDS
CTCTATTATGGCAGGTTGAA+GGG 0.459281 4.3:+85931927 None:intergenic
AGTAGTTCCAAGGTCTCATC+AGG 0.460773 4.3:-85933327 MS.gene60222:CDS
CAGAAGATCAAAAGCTTGAA+AGG 0.461492 4.3:-85932483 MS.gene60222:CDS
GTTTAGCTTGCGAAGAATCC+CGG 0.462680 4.3:-85932296 MS.gene60222:CDS
GCCGCTCAGTTTGGTAATGT+GGG 0.462843 4.3:-85931617 MS.gene60222:CDS
TTGGTTTGGAAGATGGAAAA+GGG 0.463739 4.3:-85934377 None:intergenic
TGAACCTGCAAAAGTGGAAT+TGG 0.464663 4.3:-85934123 MS.gene60222:CDS
GTAGGTTTGATGTCTAAATC+AGG 0.465650 4.3:-85933207 MS.gene60222:CDS
ACTCTATTATGGCAGGTTGA+AGG 0.468122 4.3:+85931926 None:intergenic
TGCCGCTCAGTTTGGTAATG+TGG 0.469315 4.3:-85931618 MS.gene60222:CDS
GCACTCATCCTGACTGAGAC+GGG 0.469960 4.3:+85933557 None:intergenic
GGCCTTCATCGCCAGATTCA+GGG 0.471401 4.3:-85932649 MS.gene60222:CDS
TAACATAAATTCTAAGGAAG+TGG 0.472992 4.3:-85933385 MS.gene60222:CDS
TTAAAACCAATTCAATGGGA+TGG 0.477591 4.3:-85931868 MS.gene60222:CDS
CAAATATGAAAGAAGTAAAG+AGG 0.479183 4.3:-85932804 MS.gene60222:CDS
AGAAAGATATATGTTTATGA+TGG 0.479440 4.3:-85932379 MS.gene60222:CDS
TTTGGTTTGGAAGATGGAAA+AGG 0.479837 4.3:-85934378 None:intergenic
TGTAGTTGAGTCATGTTGGC+AGG 0.480080 4.3:-85932350 MS.gene60222:CDS
GAATTCTGTTAGAGTGATAC+GGG 0.480221 4.3:-85932425 MS.gene60222:CDS
GTTGCATCCGTTTCTGCTGA+TGG 0.482158 4.3:-85934341 MS.gene60222:CDS
GTTGAATATTTATTCCAAGT+TGG 0.486063 4.3:+85931900 None:intergenic
TATAGGGCCACGTGGGAAGC+TGG 0.486372 4.3:-85932326 MS.gene60222:CDS
AACAAGTGACTGTACTTTGA+AGG 0.487630 4.3:-85934198 MS.gene60222:CDS
TCAAATGGATGAGTCTACTT+TGG 0.489113 4.3:-85933859 MS.gene60222:CDS
AATATCAATATTGGTGGTTC+CGG 0.489476 4.3:-85933675 MS.gene60222:CDS
AGAATTCTGTTAGAGTGATA+CGG 0.492353 4.3:-85932426 MS.gene60222:CDS
CAATGCTCCCCGTCTCAGTC+AGG 0.493023 4.3:-85933565 MS.gene60222:CDS
TTTATGATCATCAAGACAGC+AGG 0.495980 4.3:-85931544 MS.gene60222:CDS
AGAAGATCAAAAGCTTGAAA+GGG 0.499298 4.3:-85932482 MS.gene60222:CDS
AATGAAGGGGAGAATATCTT+GGG 0.502787 4.3:-85932733 MS.gene60222:CDS
AGTTCAAGGTTTGATGGCAG+TGG 0.503325 4.3:-85933295 MS.gene60222:CDS
TCTGTTCCAGGGGTTGAAGT+TGG 0.503371 4.3:-85932709 MS.gene60222:CDS
AAGCCAAGCTCTGGTTGACC+CGG 0.503691 4.3:+85932277 None:intergenic
AAGGGAGCTGTGTTAATGAA+GGG 0.505095 4.3:-85932747 MS.gene60222:CDS
GAAAATTGATAATGTCCAGC+TGG 0.508457 4.3:-85933157 MS.gene60222:CDS
AGTGATTTCATCTCATCAAA+TGG 0.508635 4.3:-85933874 MS.gene60222:CDS
ACTCGTACCATCAGCAGAAA+CGG 0.511526 4.3:+85934334 None:intergenic
TGTACTGGTAGGTTGTATTG+AGG 0.511536 4.3:-85931380 None:intergenic
ACCTGCAAAACTGGCATTGG+CGG 0.513521 4.3:-85933826 MS.gene60222:CDS
AGTAGTTCCAAGGTCTCATC+GGG 0.515055 4.3:-85933237 MS.gene60222:CDS
AAGGTGTACTGAATCATTCT+GGG 0.515488 4.3:-85933107 MS.gene60222:CDS
TGCAATCTTATCCCTCCAAA+AGG 0.515972 4.3:-85932091 MS.gene60222:CDS
TTTCCTGCCCGATGAGACCT+TGG 0.518275 4.3:+85933230 None:intergenic
TGGTTCCGGTGCTTGTATGA+AGG 0.518765 4.3:-85933661 MS.gene60222:CDS
TTTGATGGCAGTGGTTAAAG+AGG 0.518874 4.3:-85933286 MS.gene60222:CDS
AACAACAATAATCTTTGTGA+TGG 0.520060 4.3:-85931713 MS.gene60222:CDS
ATCCCGGGTCAACCAGAGCT+TGG 0.520207 4.3:-85932280 MS.gene60222:CDS
AAGTAGTTCCAAGGTCTCAT+CGG 0.520423 4.3:-85933238 MS.gene60222:CDS
TTGGCTAACTCTATTATGGC+AGG 0.524710 4.3:+85931919 None:intergenic
GCATTGCCTCCACATAAACT+TGG 0.526839 4.3:+85933582 None:intergenic
AAAACCAATTCAATGGGATG+GGG 0.526952 4.3:-85931866 MS.gene60222:CDS
ATCGTATGTCAGCTCTTGCA+AGG 0.529691 4.3:+85931481 None:intergenic
TTGTCCAATATCAATATTGG+TGG 0.531639 4.3:-85933681 MS.gene60222:CDS
CTCAAGTTCAAGTGAAGTAG+TGG 0.532681 4.3:-85931663 MS.gene60222:CDS
TTTAGCTTGCGAAGAATCCC+GGG 0.534568 4.3:-85932295 MS.gene60222:CDS
TTTCCTGCCTGATGAGACCT+TGG 0.536083 4.3:+85933320 None:intergenic
GAAGGGAGCTGTGTTAATGA+AGG 0.538115 4.3:-85932748 MS.gene60222:CDS
GGGTGTGGTAACTTTGGATA+CGG 0.539320 4.3:-85933805 MS.gene60222:CDS
TGAACCTGCAAAACTGGCAT+TGG 0.539713 4.3:-85933829 MS.gene60222:CDS
TCAGGCAGGAAAGTAGTTCA+AGG 0.539738 4.3:-85933309 MS.gene60222:CDS
TACTTTGAAGGAGGATCCCA+TGG 0.541291 4.3:-85934186 MS.gene60222:CDS
GATAAAACTTCCTGAAGGGA+TGG 0.541390 4.3:+85931826 None:intergenic
ATGAGAATGACTCAGTGGCC+AGG 0.542434 4.3:-85932906 MS.gene60222:CDS
CGGAACGAAAAGGAAAGAAA+GGG 0.542783 4.3:-85933183 MS.gene60222:CDS
AAGGAAGCTGAACAAAGAAC+CGG 0.544426 4.3:-85931773 MS.gene60222:CDS
GTCTCATCGGGCAGGAAAGT+AGG 0.544739 4.3:-85933225 MS.gene60222:CDS
AAATCTTTCTATGAAAAGCA+TGG 0.545950 4.3:+85932854 None:intergenic
TGGCATGGAAACTATGGATA+CGG 0.546219 4.3:-85934099 MS.gene60222:CDS
GATACGGGGCTATGAATCAA+TGG 0.551635 4.3:-85932410 MS.gene60222:CDS
TTTATGTAGTTGAGTCATGT+TGG 0.552455 4.3:-85932354 MS.gene60222:CDS
GGCACTCATCCTGACTGAGA+CGG 0.553076 4.3:+85933556 None:intergenic
TCATCACCAACTTCAACCCC+TGG 0.553210 4.3:+85932703 None:intergenic
ATTGGCGGGTGTGGTAACTT+TGG 0.553345 4.3:-85933811 MS.gene60222:CDS
AGCCTCCTTCATACAAGCAC+CGG 0.554334 4.3:+85933656 None:intergenic
TAAATCAGGACGGAACGAAA+AGG 0.554578 4.3:-85933193 MS.gene60222:CDS
ATTCAGTACACCTTTCGACT+TGG 0.555108 4.3:+85933116 None:intergenic
TAACGATGAACCTGCAAAAG+TGG 0.556879 4.3:-85934129 MS.gene60222:CDS
TCTTATCTGATCAAGTAGAT+GGG 0.557317 4.3:-85934163 MS.gene60222:CDS
CAACTAGTATTGTTTCTTCG+GGG 0.557635 4.3:-85932588 MS.gene60222:CDS
AAAACTGGCATTGGCGGGTG+TGG 0.558834 4.3:-85933820 MS.gene60222:CDS
AGGGAGCTGTGTTAATGAAG+GGG 0.559797 4.3:-85932746 MS.gene60222:CDS
TTCTAAGGAAGTGGTTAAAG+CGG 0.560454 4.3:-85933376 MS.gene60222:CDS
AACTAGTATTGTTTCTTCGG+GGG 0.564319 4.3:-85932587 MS.gene60222:CDS
GTTCCAAGGTCTCATCGGGC+AGG 0.565288 4.3:-85933233 MS.gene60222:CDS
CACAGATAAAACTTCCTGAA+GGG 0.565553 4.3:+85931822 None:intergenic
ATGGTGTTCACAGCACATAT+CGG 0.565881 4.3:+85932172 None:intergenic
GATACGGAATTTGCGACAGA+AGG 0.568390 4.3:-85933789 MS.gene60222:CDS
GGTTATGTATTTAAATGTAG+GGG 0.572990 4.3:+85932135 None:intergenic
AGCCAAGCTCTGGTTGACCC+GGG 0.573238 4.3:+85932278 None:intergenic
AAGAAACAATACTAGTTGCA+AGG 0.576224 4.3:+85932593 None:intergenic
TCACAGATAAAACTTCCTGA+AGG 0.579555 4.3:+85931821 None:intergenic
GTTCCAAGGTCTCATCAGGC+AGG 0.585792 4.3:-85933323 MS.gene60222:CDS
ATATCTTGGGATCTGTTCCA+GGG 0.585881 4.3:-85932720 MS.gene60222:CDS
AAATTGTAGTTCTGATGACA+TGG 0.587546 4.3:-85933058 MS.gene60222:CDS
TCAAAGGCATCGTATCCAGC+TGG 0.588086 4.3:-85933738 MS.gene60222:CDS
AACTGAAGGCTGTAGTCTGA+AGG 0.588098 4.3:-85933907 MS.gene60222:CDS
CCTGCAAAACTGGCATTGGC+GGG 0.589355 4.3:-85933825 MS.gene60222:CDS
ACTAGTATTGTTTCTTCGGG+GGG 0.593022 4.3:-85932586 MS.gene60222:CDS
TATGTCAGCTCTTGCAAGGG+AGG 0.595124 4.3:+85931485 None:intergenic
GACGTGGAATTTGCAACTGA+AGG 0.595906 4.3:-85933921 MS.gene60222:CDS
AAAACTCAAAAGTAGTTCCA+AGG 0.596538 4.3:-85933247 MS.gene60222:CDS
AAAACTTAAAAGTAGTTCCA+AGG 0.596538 4.3:-85933337 MS.gene60222:CDS
ACTGTTCAAGATGATTCTGA+AGG 0.600204 4.3:-85933453 MS.gene60222:CDS
GTTATGTATTTAAATGTAGG+GGG 0.600728 4.3:+85932136 None:intergenic
CGGGGCTATGAATCAATGGA+TGG 0.606352 4.3:-85932406 MS.gene60222:CDS
TTCCGGTGCTTGTATGAAGG+AGG 0.609421 4.3:-85933658 MS.gene60222:CDS
TGCTGATGGTACGAGTGTTG+AGG 0.609913 4.3:-85934327 MS.gene60222:CDS
CACTCATCCTGACTGAGACG+GGG 0.610274 4.3:+85933558 None:intergenic
GTGTCGAAGATATTTCCTAT+GGG 0.615325 4.3:-85932207 MS.gene60222:CDS
AGATTTATCCTATATATCAA+AGG 0.619809 4.3:-85933754 MS.gene60222:CDS
AAAAGTGGAATTGGTTGGCA+TGG 0.620927 4.3:-85934114 MS.gene60222:CDS
CTTATCTGATCAAGTAGATG+GGG 0.627230 4.3:-85934162 MS.gene60222:CDS
AAGTTCAAGTGAAGTAGTGG+AGG 0.627990 4.3:-85931660 MS.gene60222:CDS
GGACCACACTCATAGACTAG+AGG 0.629150 4.3:+85931962 None:intergenic
GGCATCGTATCCAGCTGGCG+AGG 0.630693 4.3:-85933733 MS.gene60222:CDS
GATTCCATTTAATCATAATG+GGG 0.631488 4.3:-85932002 MS.gene60222:CDS
CTTGGTCGTAGTAATTTGAG+TGG 0.634936 4.3:-85932931 MS.gene60222:CDS
CTGTTCAAGATGATTCTGAA+GGG 0.636210 4.3:-85933452 MS.gene60222:CDS
GAAGATCAAAAGCTTGAAAG+GGG 0.636952 4.3:-85932481 MS.gene60222:CDS
ACTTGATCAGATAAGACCAT+GGG 0.639082 4.3:+85934170 None:intergenic
CAGTCAGGATGAGTGCCGAA+AGG 0.642494 4.3:-85933550 MS.gene60222:CDS
ACATAATCATCATCATCATG+AGG 0.642724 4.3:-85933409 MS.gene60222:CDS
CCTGCAAAAGTGGAATTGGT+TGG 0.646230 4.3:-85934119 MS.gene60222:CDS
TCGTATGTCAGCTCTTGCAA+GGG 0.649908 4.3:+85931482 None:intergenic
AAGTGACTGTACTTTGAAGG+AGG 0.650182 4.3:-85934195 MS.gene60222:CDS
CCGCTCAGTTTGGTAATGTG+GGG 0.650291 4.3:-85931616 MS.gene60222:CDS
ACATTCAGGATATATCATCT+CGG 0.654068 4.3:+85932114 None:intergenic
TACTTGATCAGATAAGACCA+TGG 0.655790 4.3:+85934169 None:intergenic
AAAACTGCTACCAAGTCGAA+AGG 0.659857 4.3:-85933126 MS.gene60222:CDS
TTATGATCATCAAGACAGCA+GGG 0.660006 4.3:-85931543 MS.gene60222:CDS
GATACAGTTCGTGATTCCGA+TGG 0.661038 4.3:-85931443 MS.gene60222:CDS
CTCAAATTACTACGACCAAG+AGG 0.664835 4.3:+85932934 None:intergenic
ACGAGTGTTGAGGATAAGAG+TGG 0.665431 4.3:-85934317 MS.gene60222:CDS
TGCTTTGAAGAACAGTAGTG+AGG 0.666121 4.3:-85932452 MS.gene60222:CDS
TATCTTGGGATCTGTTCCAG+GGG 0.671761 4.3:-85932719 MS.gene60222:CDS
TTTGTTTGTTACAAACACTG+TGG 0.672214 4.3:-85934225 MS.gene60222:CDS
GTTCTGTGGAATGTACTGGT+AGG 0.675695 4.3:-85931391 MS.gene60222:CDS
CAGTGGCCAGGAATAAAGTG+AGG 0.677717 4.3:-85932894 MS.gene60222:CDS
TATCTGTGAATATATTGGCG+AGG 0.678899 4.3:-85931807 MS.gene60222:CDS
GCAGCAAATAACATGATGTG+AGG 0.681849 4.3:+85931518 None:intergenic
GGCAGGATATAGGGCCACGT+GGG 0.686351 4.3:-85932333 MS.gene60222:CDS
AAGCCTCTAGTCTATGAGTG+TGG 0.686595 4.3:-85931965 MS.gene60222:CDS
TTGATATATACAGGGCAGGG+TGG 0.688777 4.3:-85932532 MS.gene60222:CDS
GTTTGATGTCTAAATCAGGA+CGG 0.690095 4.3:-85933203 MS.gene60222:CDS
AATTGTAGTTCTGATGACAT+GGG 0.693133 4.3:-85933057 MS.gene60222:CDS
TGGCAGGATATAGGGCCACG+TGG 0.698447 4.3:-85932334 MS.gene60222:CDS
AAATTGATGAGATGAGACCA+TGG 0.705903 4.3:+85934037 None:intergenic
ATTCTGAGCATAAAGATTAG+GGG 0.715506 4.3:+85931571 None:intergenic
CCCCACATTACCAAACTGAG+CGG 0.724747 4.3:+85931616 None:intergenic
CTTCATCGCCAGATTCAGGG+TGG 0.729437 4.3:-85932646 MS.gene60222:CDS
TTATAAACCAGCTTCCCACG+TGG 0.745113 4.3:+85932319 None:intergenic
TGGTCATGAGAATGACTCAG+TGG 0.759725 4.3:-85932911 MS.gene60222:CDS
AATATTGATATTGGACAACG+AGG 0.767583 4.3:+85933686 None:intergenic
AATTCTGTTAGAGTGATACG+GGG 0.776687 4.3:-85932424 MS.gene60222:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTTAAAACCAATTCAA+TGG - chr4.3:85933874-85933893 MS.gene60222:CDS 15.0%
!!! ATTTTTAAAACCAATTCAAT+GGG - chr4.3:85933875-85933894 MS.gene60222:CDS 15.0%
!! AGAAAGATATATGTTTATGA+TGG - chr4.3:85933368-85933387 MS.gene60222:CDS 20.0%
!! AGAATAGAGCTTAATATAAT+TGG - chr4.3:85933077-85933096 MS.gene60222:CDS 20.0%
!! AGATTCCATTTAATCATAAT+GGG - chr4.3:85933744-85933763 MS.gene60222:CDS 20.0%
!! AGATTTATCCTATATATCAA+AGG - chr4.3:85931993-85932012 MS.gene60222:CDS 20.0%
!!! AAGTGTTTGTTATTTTCATT+AGG + chr4.3:85932750-85932769 None:intergenic 20.0%
!!! AGTGTTTGTTATTTTCATTA+GGG + chr4.3:85932749-85932768 None:intergenic 20.0%
!!! AGTTTTATCTGTGAATATAT+TGG - chr4.3:85933935-85933954 MS.gene60222:CDS 20.0%
!!! ATAGGATAAATCTTGTTTTA+CGG + chr4.3:85931986-85932005 None:intergenic 20.0%
!!! TTTTCTCTTTTCTTTTTCAA+CGG + chr4.3:85932672-85932691 None:intergenic 20.0%
! AAAAAGTTCAAAACAAGAGA+AGG - chr4.3:85933512-85933531 MS.gene60222:CDS 25.0%
! AAAACTTAAAAGTAGTTCCA+AGG - chr4.3:85932410-85932429 MS.gene60222:CDS 25.0%
! AAAGGATGTAATTGTACTAA+TGG - chr4.3:85933674-85933693 MS.gene60222:CDS 25.0%
! AAATCTTTCTATGAAAAGCA+TGG + chr4.3:85932896-85932915 None:intergenic 25.0%
! AACAACAATAATCTTTGTGA+TGG - chr4.3:85934034-85934053 MS.gene60222:CDS 25.0%
! ATATCCCCATTATGATTAAA+TGG + chr4.3:85933752-85933771 None:intergenic 25.0%
! CAAATATGAAAGAAGTAAAG+AGG - chr4.3:85932943-85932962 MS.gene60222:CDS 25.0%
! CGGTTATGTATTTAAATGTA+GGG + chr4.3:85933616-85933635 None:intergenic 25.0%
! GAGATTCCATTTAATCATAA+TGG - chr4.3:85933743-85933762 MS.gene60222:CDS 25.0%
! GATTCCATTTAATCATAATG+GGG - chr4.3:85933745-85933764 MS.gene60222:CDS 25.0%
! GGTTATGTATTTAAATGTAG+GGG + chr4.3:85933615-85933634 None:intergenic 25.0%
! GTTATGTATTTAAATGTAGG+GGG + chr4.3:85933614-85933633 None:intergenic 25.0%
! GTTGAATATTTATTCCAAGT+TGG + chr4.3:85933850-85933869 None:intergenic 25.0%
! TCGGTTATGTATTTAAATGT+AGG + chr4.3:85933617-85933636 None:intergenic 25.0%
! TGAGGTTAACATAAATTCTA+AGG - chr4.3:85932356-85932375 MS.gene60222:CDS 25.0%
!! ACTTCTTTCATATTTGACTT+TGG + chr4.3:85932939-85932958 None:intergenic 25.0%
!! TAACATAAATTCTAAGGAAG+TGG - chr4.3:85932362-85932381 MS.gene60222:CDS 25.0%
!! TTAGTACAATTACATCCTTT+TGG + chr4.3:85933674-85933693 None:intergenic 25.0%
!! TTTTCATAGAAAGATTTTGC+AGG - chr4.3:85932899-85932918 MS.gene60222:CDS 25.0%
!!! CAGATGTTTTGATATATACA+GGG - chr4.3:85933207-85933226 MS.gene60222:CDS 25.0%
!!! TCAGATGTTTTGATATATAC+AGG - chr4.3:85933206-85933225 MS.gene60222:CDS 25.0%
!!! TGATGATGATTATGTTTTTC+AGG + chr4.3:85932333-85932352 None:intergenic 25.0%
AAAACTCAAAAGTAGTTCCA+AGG - chr4.3:85932500-85932519 MS.gene60222:CDS 30.0%
AAGAAACAATACTAGTTGCA+AGG + chr4.3:85933157-85933176 None:intergenic 30.0%
AATATTGATATTGGACAACG+AGG + chr4.3:85932064-85932083 None:intergenic 30.0%
ACATAATCATCATCATCATG+AGG - chr4.3:85932338-85932357 MS.gene60222:CDS 30.0%
ACATTCAGGATATATCATCT+CGG + chr4.3:85933636-85933655 None:intergenic 30.0%
ACATTCTGAGCATAAAGATT+AGG + chr4.3:85934181-85934200 None:intergenic 30.0%
AGAAGATCAAAAGCTTGAAA+GGG - chr4.3:85933265-85933284 MS.gene60222:CDS 30.0%
AGAATTCTGTTAGAGTGATA+CGG - chr4.3:85933321-85933340 MS.gene60222:CDS 30.0%
AGTGATTTCATCTCATCAAA+TGG - chr4.3:85931873-85931892 MS.gene60222:CDS 30.0%
ATACGATGCCTTTGATATAT+AGG + chr4.3:85932004-85932023 None:intergenic 30.0%
ATTCTGAGCATAAAGATTAG+GGG + chr4.3:85934179-85934198 None:intergenic 30.0%
CATTCTGAGCATAAAGATTA+GGG + chr4.3:85934180-85934199 None:intergenic 30.0%
CTTGAAAAAGGAATATACCA+TGG - chr4.3:85931693-85931712 MS.gene60222:CDS 30.0%
GAAAAGATACTCATCATTAC+CGG + chr4.3:85933996-85934015 None:intergenic 30.0%
TAAAACCAATTCAATGGGAT+GGG - chr4.3:85933880-85933899 MS.gene60222:CDS 30.0%
TCGTTGTCCAATATCAATAT+TGG - chr4.3:85932063-85932082 MS.gene60222:CDS 30.0%
TCTTATCTGATCAAGTAGAT+GGG - chr4.3:85931584-85931603 MS.gene60222:CDS 30.0%
TTAAAACCAATTCAATGGGA+TGG - chr4.3:85933879-85933898 MS.gene60222:CDS 30.0%
TTGTCCAATATCAATATTGG+TGG - chr4.3:85932066-85932085 MS.gene60222:CDS 30.0%
TTTATGTAGTTGAGTCATGT+TGG - chr4.3:85933393-85933412 MS.gene60222:CDS 30.0%
! AATATCAATATTGGTGGTTC+CGG - chr4.3:85932072-85932091 MS.gene60222:CDS 30.0%
! AGAGATTTTCCAAGTTTATG+TGG - chr4.3:85932156-85932175 MS.gene60222:CDS 30.0%
! CTTCTTTTTGATATTGCCAT+CGG + chr4.3:85934323-85934342 None:intergenic 30.0%
! GATGAGTATCTTTTCGATAT+CGG - chr4.3:85934001-85934020 MS.gene60222:CDS 30.0%
! TACAATTACATCCTTTTGGA+GGG + chr4.3:85933670-85933689 None:intergenic 30.0%
! TCGTTCAACTCAAAATTTTG+AGG + chr4.3:85931499-85931518 None:intergenic 30.0%
! TGCAACTAGTATTGTTTCTT+CGG - chr4.3:85933157-85933176 MS.gene60222:CDS 30.0%
! TTTGTTTGTTACAAACACTG+TGG - chr4.3:85931522-85931541 MS.gene60222:CDS 30.0%
!! AAATTGTAGTTCTGATGACA+TGG - chr4.3:85932689-85932708 MS.gene60222:CDS 30.0%
!! AATTGTAGTTCTGATGACAT+GGG - chr4.3:85932690-85932709 MS.gene60222:CDS 30.0%
!! CATGGTATATTCCTTTTTCA+AGG + chr4.3:85931695-85931714 None:intergenic 30.0%
!! TATTTTAGAGACTTTCGATG+TGG - chr4.3:85931741-85931760 MS.gene60222:CDS 30.0%
!!! AAAAAGAAGTACTGCTTTTG+TGG - chr4.3:85934334-85934353 MS.gene60222:CDS 30.0%
!!! ATTTGACTTTGGTTTTGCTT+CGG + chr4.3:85932928-85932947 None:intergenic 30.0%
!!! TCAAAATTTTGAGTTGAACG+AGG - chr4.3:85931498-85931517 MS.gene60222:CDS 30.0%
AAAACCAATTCAATGGGATG+GGG - chr4.3:85933881-85933900 MS.gene60222:CDS 35.0%
AAATTGATGAGATGAGACCA+TGG + chr4.3:85931713-85931732 None:intergenic 35.0%
AACAAGTGACTGTACTTTGA+AGG - chr4.3:85931549-85931568 MS.gene60222:CDS 35.0%
AAGGTGTACTGAATCATTCT+GGG - chr4.3:85932640-85932659 MS.gene60222:CDS 35.0%
AATGAAGGGGAGAATATCTT+GGG - chr4.3:85933014-85933033 MS.gene60222:CDS 35.0%
AATTCTGTTAGAGTGATACG+GGG - chr4.3:85933323-85933342 MS.gene60222:CDS 35.0%
ACGTCAAAAGTCTCCAAAAT+AGG + chr4.3:85931811-85931830 None:intergenic 35.0%
ACTGTTCAAGATGATTCTGA+AGG - chr4.3:85932294-85932313 MS.gene60222:CDS 35.0%
ACTTGATCAGATAAGACCAT+GGG + chr4.3:85931580-85931599 None:intergenic 35.0%
CACAGATAAAACTTCCTGAA+GGG + chr4.3:85933928-85933947 None:intergenic 35.0%
CAGAAGATCAAAAGCTTGAA+AGG - chr4.3:85933264-85933283 MS.gene60222:CDS 35.0%
CATAATAGAGTTAGCCAACT+TGG - chr4.3:85933833-85933852 MS.gene60222:CDS 35.0%
CTGTTCAAGATGATTCTGAA+GGG - chr4.3:85932295-85932314 MS.gene60222:CDS 35.0%
CTTATCTGATCAAGTAGATG+GGG - chr4.3:85931585-85931604 MS.gene60222:CDS 35.0%
GAAAGTAGTTCAAGGTTTGA+TGG - chr4.3:85932446-85932465 MS.gene60222:CDS 35.0%
GAAGATCAAAAGCTTGAAAG+GGG - chr4.3:85933266-85933285 MS.gene60222:CDS 35.0%
GAATTCTGTTAGAGTGATAC+GGG - chr4.3:85933322-85933341 MS.gene60222:CDS 35.0%
GGAACCACCAATATTGATAT+TGG + chr4.3:85932073-85932092 None:intergenic 35.0%
GTACGCAACAACATTTCTTA+TGG + chr4.3:85933709-85933728 None:intergenic 35.0%
GTAGGTTTGATGTCTAAATC+AGG - chr4.3:85932540-85932559 MS.gene60222:CDS 35.0%
GTCTTATCTGATCAAGTAGA+TGG - chr4.3:85931583-85931602 MS.gene60222:CDS 35.0%
GTGTCGAAGATATTTCCTAT+GGG - chr4.3:85933540-85933559 MS.gene60222:CDS 35.0%
GTTTGATGTCTAAATCAGGA+CGG - chr4.3:85932544-85932563 MS.gene60222:CDS 35.0%
TAATGAAGGGGAGAATATCT+TGG - chr4.3:85933013-85933032 MS.gene60222:CDS 35.0%
TACTTGATCAGATAAGACCA+TGG + chr4.3:85931581-85931600 None:intergenic 35.0%
TATCTGTGAATATATTGGCG+AGG - chr4.3:85933940-85933959 MS.gene60222:CDS 35.0%
TCAAAGAAGAAAGCTCCTTT+CGG + chr4.3:85932215-85932234 None:intergenic 35.0%
TCAAATGGATGAGTCTACTT+TGG - chr4.3:85931888-85931907 MS.gene60222:CDS 35.0%
TCACAGATAAAACTTCCTGA+AGG + chr4.3:85933929-85933948 None:intergenic 35.0%
TGAAGACTGTACCTTGAAAA+AGG - chr4.3:85931681-85931700 MS.gene60222:CDS 35.0%
TGAGTCAGAATCTGGAAAAA+GGG + chr4.3:85934068-85934087 None:intergenic 35.0%
TGTGTCGAAGATATTTCCTA+TGG - chr4.3:85933539-85933558 MS.gene60222:CDS 35.0%
TGTTGTTGCGTACTCAAAAA+TGG - chr4.3:85933716-85933735 MS.gene60222:CDS 35.0%
TTATGATCATCAAGACAGCA+GGG - chr4.3:85934204-85934223 MS.gene60222:CDS 35.0%
TTGAGTCAGAATCTGGAAAA+AGG + chr4.3:85934069-85934088 None:intergenic 35.0%
TTTATGATCATCAAGACAGC+AGG - chr4.3:85934203-85934222 MS.gene60222:CDS 35.0%
! AAAGGTGTACTGAATCATTC+TGG - chr4.3:85932639-85932658 MS.gene60222:CDS 35.0%
! AACTAGTATTGTTTCTTCGG+GGG - chr4.3:85933160-85933179 MS.gene60222:CDS 35.0%
! AGCGTAAATGTTTTTCCTCT+TGG - chr4.3:85932798-85932817 MS.gene60222:CDS 35.0%
! CAACTAGTATTGTTTCTTCG+GGG - chr4.3:85933159-85933178 MS.gene60222:CDS 35.0%
! CAAGTTGGCTAACTCTATTA+TGG + chr4.3:85933835-85933854 None:intergenic 35.0%
! CTTTCAAGCTTTTGATCTTC+TGG + chr4.3:85933266-85933285 None:intergenic 35.0%
! GATTTTCCAAGTTTATGTGG+AGG - chr4.3:85932159-85932178 MS.gene60222:CDS 35.0%
! GCAACTAGTATTGTTTCTTC+GGG - chr4.3:85933158-85933177 MS.gene60222:CDS 35.0%
! GTACAATTACATCCTTTTGG+AGG + chr4.3:85933671-85933690 None:intergenic 35.0%
! TGAAGGAGGCTTTAACTAAA+AGG - chr4.3:85932103-85932122 MS.gene60222:CDS 35.0%
! TGAGGATACTTTTCTTCGTT+GGG + chr4.3:85931481-85931500 None:intergenic 35.0%
! TGCTGTCTTGATGATCATAA+AGG + chr4.3:85934204-85934223 None:intergenic 35.0%
! TGTTTTCCTCACTTTATTCC+TGG + chr4.3:85932862-85932881 None:intergenic 35.0%
! TTCTAAGGAAGTGGTTAAAG+CGG - chr4.3:85932371-85932390 MS.gene60222:CDS 35.0%
! TTCTATGAAAAGCATGGAAC+AGG + chr4.3:85932890-85932909 None:intergenic 35.0%
! TTGAGGATACTTTTCTTCGT+TGG + chr4.3:85931482-85931501 None:intergenic 35.0%
!! GAAAATTGATAATGTCCAGC+TGG - chr4.3:85932590-85932609 MS.gene60222:CDS 35.0%
!! GTTTTGATATATACAGGGCA+GGG - chr4.3:85933212-85933231 MS.gene60222:CDS 35.0%
!! TATTTTGGAGACTTTTGACG+TGG - chr4.3:85931810-85931829 MS.gene60222:CDS 35.0%
!!! AACACTGCAACTTTTTTACG+AGG + chr4.3:85932135-85932154 None:intergenic 35.0%
!!! TGTTTTGATATATACAGGGC+AGG - chr4.3:85933211-85933230 MS.gene60222:CDS 35.0%
AAAACTGCTACCAAGTCGAA+AGG - chr4.3:85932621-85932640 MS.gene60222:CDS 40.0%
AAAAGTGGAATTGGTTGGCA+TGG - chr4.3:85931633-85931652 MS.gene60222:CDS 40.0%
AAACGATGAACCTGCAAAAC+TGG - chr4.3:85931912-85931931 MS.gene60222:CDS 40.0%
AAAGATTAGGGGAGCAACTA+TGG + chr4.3:85934168-85934187 None:intergenic 40.0%
AAGGAAGCTGAACAAAGAAC+CGG - chr4.3:85933974-85933993 MS.gene60222:CDS 40.0%
AAGGGAGCTGTGTTAATGAA+GGG - chr4.3:85933000-85933019 MS.gene60222:CDS 40.0%
AAGTAGTTCCAAGGTCTCAT+CGG - chr4.3:85932419-85932438 MS.gene60222:CDS 40.0%
AAGTTCAAGTGAAGTAGTGG+AGG - chr4.3:85934087-85934106 MS.gene60222:CDS 40.0%
AATATCTTGGGATCTGTTCC+AGG - chr4.3:85933026-85933045 MS.gene60222:CDS 40.0%
AATCTATACCACCCTGAATC+TGG + chr4.3:85933112-85933131 None:intergenic 40.0%
ACAAGCTGCGAAAAAGCTTA+AGG - chr4.3:85932974-85932993 MS.gene60222:CDS 40.0%
ACGGAACGAAAAGGAAAGAA+AGG - chr4.3:85932563-85932582 MS.gene60222:CDS 40.0%
AGTCATGTTGGCAGGATATA+GGG - chr4.3:85933405-85933424 MS.gene60222:CDS 40.0%
ATATCTTGGGATCTGTTCCA+GGG - chr4.3:85933027-85933046 MS.gene60222:CDS 40.0%
ATGGTGTTCACAGCACATAT+CGG + chr4.3:85933578-85933597 None:intergenic 40.0%
ATTCAGTACACCTTTCGACT+TGG + chr4.3:85932634-85932653 None:intergenic 40.0%
ATTGGTTGGCATGGAAACTA+TGG - chr4.3:85931642-85931661 MS.gene60222:CDS 40.0%
CGAAAAAGCTTAAGGAGGAA+GGG - chr4.3:85932982-85933001 MS.gene60222:CDS 40.0%
CGGAACGAAAAGGAAAGAAA+GGG - chr4.3:85932564-85932583 MS.gene60222:CDS 40.0%
CTCAAATTACTACGACCAAG+AGG + chr4.3:85932816-85932835 None:intergenic 40.0%
CTCAAGTTCAAGTGAAGTAG+TGG - chr4.3:85934084-85934103 MS.gene60222:CDS 40.0%
CTTGAACTTGAGTCAGAATC+TGG + chr4.3:85934076-85934095 None:intergenic 40.0%
CTTGGTCGTAGTAATTTGAG+TGG - chr4.3:85932816-85932835 MS.gene60222:CDS 40.0%
GATAAAACTTCCTGAAGGGA+TGG + chr4.3:85933924-85933943 None:intergenic 40.0%
GATAAGAGTGGCAAAGATTC+AGG - chr4.3:85931442-85931461 MS.gene60222:CDS 40.0%
GCAGCAAATAACATGATGTG+AGG + chr4.3:85934232-85934251 None:intergenic 40.0%
GGGATAAGATTGCAACATTC+AGG + chr4.3:85933650-85933669 None:intergenic 40.0%
GTTGCGTACTCAAAAATGGA+GGG - chr4.3:85933720-85933739 MS.gene60222:CDS 40.0%
TAAATCAGGACGGAACGAAA+AGG - chr4.3:85932554-85932573 MS.gene60222:CDS 40.0%
TAACGATGAACCTGCAAAAG+TGG - chr4.3:85931618-85931637 MS.gene60222:CDS 40.0%
TCAAGTAGATGGGGATAGTT+TGG - chr4.3:85931594-85931613 MS.gene60222:CDS 40.0%
TCCTGAAGGGATGGAATTTA+AGG + chr4.3:85933915-85933934 None:intergenic 40.0%
TCCTTAAATTCCATCCCTTC+AGG - chr4.3:85933911-85933930 MS.gene60222:CDS 40.0%
TGAACCTGCAAAAGTGGAAT+TGG - chr4.3:85931624-85931643 MS.gene60222:CDS 40.0%
TGCAATCTTATCCCTCCAAA+AGG - chr4.3:85933656-85933675 MS.gene60222:CDS 40.0%
TGGCATGGAAACTATGGATA+CGG - chr4.3:85931648-85931667 MS.gene60222:CDS 40.0%
TGTTGCGTACTCAAAAATGG+AGG - chr4.3:85933719-85933738 MS.gene60222:CDS 40.0%
TTGCGTACTCAAAAATGGAG+GGG - chr4.3:85933721-85933740 MS.gene60222:CDS 40.0%
TTGGCTAACTCTATTATGGC+AGG + chr4.3:85933831-85933850 None:intergenic 40.0%
! AAGTGACTGTACTTTGAAGG+AGG - chr4.3:85931552-85931571 MS.gene60222:CDS 40.0%
! ACGCTCGAACTATGTGATTT+TGG + chr4.3:85932783-85932802 None:intergenic 40.0%
! ACTAGTATTGTTTCTTCGGG+GGG - chr4.3:85933161-85933180 MS.gene60222:CDS 40.0%
! ACTCTATTATGGCAGGTTGA+AGG + chr4.3:85933824-85933843 None:intergenic 40.0%
! CGCTCGAACTATGTGATTTT+GGG + chr4.3:85932782-85932801 None:intergenic 40.0%
! CGGAATTTTCTCTTTCCCAT+AGG + chr4.3:85933558-85933577 None:intergenic 40.0%
! CTCTATTATGGCAGGTTGAA+GGG + chr4.3:85933823-85933842 None:intergenic 40.0%
! TTCTGCTTCTTTTACGTCAG+TGG + chr4.3:85932250-85932269 None:intergenic 40.0%
! TTTGATGGCAGTGGTTAAAG+AGG - chr4.3:85932461-85932480 MS.gene60222:CDS 40.0%
!! TGCTTTGAAGAACAGTAGTG+AGG - chr4.3:85933295-85933314 MS.gene60222:CDS 40.0%
AACTGAAGGCTGTAGTCTGA+AGG - chr4.3:85931840-85931859 MS.gene60222:CDS 45.0%
AAGCCTCTAGTCTATGAGTG+TGG - chr4.3:85933782-85933801 MS.gene60222:CDS 45.0%
ACTCGTACCATCAGCAGAAA+CGG + chr4.3:85931416-85931435 None:intergenic 45.0%
AGCTGCGAAAAAGCTTAAGG+AGG - chr4.3:85932977-85932996 MS.gene60222:CDS 45.0%
AGGGAGCTGTGTTAATGAAG+GGG - chr4.3:85933001-85933020 MS.gene60222:CDS 45.0%
AGGGGGTTTCTCGTTATCTA+TGG + chr4.3:85933597-85933616 None:intergenic 45.0%
AGTAGTTCCAAGGTCTCATC+AGG - chr4.3:85932420-85932439 MS.gene60222:CDS 45.0%
AGTAGTTCCAAGGTCTCATC+GGG - chr4.3:85932420-85932439 MS.gene60222:CDS 45.0%
ATCGTATGTCAGCTCTTGCA+AGG + chr4.3:85934269-85934288 None:intergenic 45.0%
CCTGCAAAAGTGGAATTGGT+TGG - chr4.3:85931628-85931647 MS.gene60222:CDS 45.0%
GAAGGGAGCTGTGTTAATGA+AGG - chr4.3:85932999-85933018 MS.gene60222:CDS 45.0%
GACGTGGAATTTGCAACTGA+AGG - chr4.3:85931826-85931845 MS.gene60222:CDS 45.0%
GAGTCATGTTGGCAGGATAT+AGG - chr4.3:85933404-85933423 MS.gene60222:CDS 45.0%
GATACAGTTCGTGATTCCGA+TGG - chr4.3:85934304-85934323 MS.gene60222:CDS 45.0%
GATACGGAATTTGCGACAGA+AGG - chr4.3:85931958-85931977 MS.gene60222:CDS 45.0%
GATACGGGGCTATGAATCAA+TGG - chr4.3:85933337-85933356 MS.gene60222:CDS 45.0%
GCATTGCCTCCACATAAACT+TGG + chr4.3:85932168-85932187 None:intergenic 45.0%
GCGAAAAAGCTTAAGGAGGA+AGG - chr4.3:85932981-85933000 MS.gene60222:CDS 45.0%
GGGTGTGGTAACTTTGGATA+CGG - chr4.3:85931942-85931961 MS.gene60222:CDS 45.0%
TACACCCCATCCCATTGAAT+TGG + chr4.3:85933888-85933907 None:intergenic 45.0%
TATCTTGGGATCTGTTCCAG+GGG - chr4.3:85933028-85933047 MS.gene60222:CDS 45.0%
TCAGGCAGGAAAGTAGTTCA+AGG - chr4.3:85932438-85932457 MS.gene60222:CDS 45.0%
TCGTATGTCAGCTCTTGCAA+GGG + chr4.3:85934268-85934287 None:intergenic 45.0%
TGAACCTGCAAAACTGGCAT+TGG - chr4.3:85931918-85931937 MS.gene60222:CDS 45.0%
TGGCGAGGTTCTTGAAGAAA+AGG - chr4.3:85933955-85933974 MS.gene60222:CDS 45.0%
TGGTCATGAGAATGACTCAG+TGG - chr4.3:85932836-85932855 MS.gene60222:CDS 45.0%
TGTAGTTGAGTCATGTTGGC+AGG - chr4.3:85933397-85933416 MS.gene60222:CDS 45.0%
TTATAAACCAGCTTCCCACG+TGG + chr4.3:85933431-85933450 None:intergenic 45.0%
TTGATATATACAGGGCAGGG+TGG - chr4.3:85933215-85933234 MS.gene60222:CDS 45.0%
! AGTTCAAGGTTTGATGGCAG+TGG - chr4.3:85932452-85932471 MS.gene60222:CDS 45.0%
! CGAAGACGATGTGCCTATTT+TGG - chr4.3:85931795-85931814 MS.gene60222:CDS 45.0%
! GTTCTGTGGAATGTACTGGT+AGG - chr4.3:85934356-85934375 MS.gene60222:CDS 45.0%
! TACTTTGAAGGAGGATCCCA+TGG - chr4.3:85931561-85931580 MS.gene60222:CDS 45.0%
! TGTGGTTCTGTGGAATGTAC+TGG - chr4.3:85934352-85934371 MS.gene60222:CDS 45.0%
!! ACGAGTGTTGAGGATAAGAG+TGG - chr4.3:85931430-85931449 MS.gene60222:CDS 45.0%
!! CCAACCAATTCCACTTTTGC+AGG + chr4.3:85931631-85931650 None:intergenic 45.0%
!! GTACTGCTTTTGTGGTTCTG+TGG - chr4.3:85934342-85934361 MS.gene60222:CDS 45.0%
!! GTTGAAGGGCACTTGCATTT+AGG + chr4.3:85933809-85933828 None:intergenic 45.0%
!! GTTTAGCTTGCGAAGAATCC+CGG - chr4.3:85933451-85933470 MS.gene60222:CDS 45.0%
!! TTGATAATGTCCAGCTGGAC+AGG - chr4.3:85932595-85932614 MS.gene60222:CDS 45.0%
!! TTTAGCTTGCGAAGAATCCC+GGG - chr4.3:85933452-85933471 MS.gene60222:CDS 45.0%
ACTATCGATGCCGCTCAGTT+TGG - chr4.3:85934121-85934140 MS.gene60222:CDS 50.0%
ACTTCCTTCAAGCCAAGCTC+TGG + chr4.3:85933482-85933501 None:intergenic 50.0%
AGCCTCCTTCATACAAGCAC+CGG + chr4.3:85932094-85932113 None:intergenic 50.0%
ATGAGAATGACTCAGTGGCC+AGG - chr4.3:85932841-85932860 MS.gene60222:CDS 50.0%
CAGTGGCCAGGAATAAAGTG+AGG - chr4.3:85932853-85932872 MS.gene60222:CDS 50.0%
CCCCACATTACCAAACTGAG+CGG + chr4.3:85934134-85934153 None:intergenic 50.0%
CGGGGCTATGAATCAATGGA+TGG - chr4.3:85933341-85933360 MS.gene60222:CDS 50.0%
GGACCACACTCATAGACTAG+AGG + chr4.3:85933788-85933807 None:intergenic 50.0%
GTTGCATCCGTTTCTGCTGA+TGG - chr4.3:85931406-85931425 MS.gene60222:CDS 50.0%
TATGTCAGCTCTTGCAAGGG+AGG + chr4.3:85934265-85934284 None:intergenic 50.0%
TCATCACCAACTTCAACCCC+TGG + chr4.3:85933047-85933066 None:intergenic 50.0%
TCTGTTCCAGGGGTTGAAGT+TGG - chr4.3:85933038-85933057 MS.gene60222:CDS 50.0%
TGGTTCCGGTGCTTGTATGA+AGG - chr4.3:85932086-85932105 MS.gene60222:CDS 50.0%
TTCCGGTGCTTGTATGAAGG+AGG - chr4.3:85932089-85932108 MS.gene60222:CDS 50.0%
TTTCCTGCCTGATGAGACCT+TGG + chr4.3:85932430-85932449 None:intergenic 50.0%
! ACCTGCAAAACTGGCATTGG+CGG - chr4.3:85931921-85931940 MS.gene60222:CDS 50.0%
! ATTGGCGGGTGTGGTAACTT+TGG - chr4.3:85931936-85931955 MS.gene60222:CDS 50.0%
! TCAACCAGAGCTTGGCTTGA+AGG - chr4.3:85933475-85933494 MS.gene60222:CDS 50.0%
! TGCTGATGGTACGAGTGTTG+AGG - chr4.3:85931420-85931439 MS.gene60222:CDS 50.0%
!! CCGCTCAGTTTGGTAATGTG+GGG - chr4.3:85934131-85934150 MS.gene60222:CDS 50.0%
!! GCCGCTCAGTTTGGTAATGT+GGG - chr4.3:85934130-85934149 MS.gene60222:CDS 50.0%
!! TCAAAGGCATCGTATCCAGC+TGG - chr4.3:85932009-85932028 MS.gene60222:CDS 50.0%
!! TGCCGCTCAGTTTGGTAATG+TGG - chr4.3:85934129-85934148 MS.gene60222:CDS 50.0%
!!! CAGTTTTAGACCTGTCCAGC+TGG + chr4.3:85932608-85932627 None:intergenic 50.0%
CACCCTGAATCTGGCGATGA+AGG + chr4.3:85933103-85933122 None:intergenic 55.0%
CACTCATCCTGACTGAGACG+GGG + chr4.3:85932192-85932211 None:intergenic 55.0%
CTTCATCGCCAGATTCAGGG+TGG - chr4.3:85933101-85933120 MS.gene60222:CDS 55.0%
GCACTCATCCTGACTGAGAC+GGG + chr4.3:85932193-85932212 None:intergenic 55.0%
GGCACTCATCCTGACTGAGA+CGG + chr4.3:85932194-85932213 None:intergenic 55.0%
GGCCTTCATCGCCAGATTCA+GGG - chr4.3:85933098-85933117 MS.gene60222:CDS 55.0%
GTCTCATCGGGCAGGAAAGT+AGG - chr4.3:85932522-85932541 MS.gene60222:CDS 55.0%
GTTCCAAGGTCTCATCAGGC+AGG - chr4.3:85932424-85932443 MS.gene60222:CDS 55.0%
TGGCCTTCATCGCCAGATTC+AGG - chr4.3:85933097-85933116 MS.gene60222:CDS 55.0%
TTTCCTGCCCGATGAGACCT+TGG + chr4.3:85932520-85932539 None:intergenic 55.0%
! AAGCCAAGCTCTGGTTGACC+CGG + chr4.3:85933473-85933492 None:intergenic 55.0%
!! AAAACTGGCATTGGCGGGTG+TGG - chr4.3:85931927-85931946 MS.gene60222:CDS 55.0%
!! CAGTCAGGATGAGTGCCGAA+AGG - chr4.3:85932197-85932216 MS.gene60222:CDS 55.0%
!! CCTGCAAAACTGGCATTGGC+GGG - chr4.3:85931922-85931941 MS.gene60222:CDS 55.0%
ATCCCGGGTCAACCAGAGCT+TGG - chr4.3:85933467-85933486 MS.gene60222:CDS 60.0%
CAATGCTCCCCGTCTCAGTC+AGG - chr4.3:85932182-85932201 MS.gene60222:CDS 60.0%
GGCAGGATATAGGGCCACGT+GGG - chr4.3:85933414-85933433 MS.gene60222:CDS 60.0%
GTTCCAAGGTCTCATCGGGC+AGG - chr4.3:85932514-85932533 MS.gene60222:CDS 60.0%
TATAGGGCCACGTGGGAAGC+TGG - chr4.3:85933421-85933440 MS.gene60222:CDS 60.0%
TGGCAGGATATAGGGCCACG+TGG - chr4.3:85933413-85933432 MS.gene60222:CDS 60.0%
! AGCCAAGCTCTGGTTGACCC+GGG + chr4.3:85933472-85933491 None:intergenic 60.0%
! CCCGCCAATGCCAGTTTTGC+AGG + chr4.3:85931925-85931944 None:intergenic 60.0%
GACATAGCTGCCTCGCCAGC+TGG + chr4.3:85932027-85932046 None:intergenic 65.0%
GGCATCGTATCCAGCTGGCG+AGG - chr4.3:85932014-85932033 MS.gene60222:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 85931382 85934387 85931382 ID=MS.gene60222
chr4.3 mRNA 85931382 85934387 85931382 ID=MS.gene60222.t1;Parent=MS.gene60222
chr4.3 exon 85931382 85934387 85931382 ID=MS.gene60222.t1.exon1;Parent=MS.gene60222.t1
chr4.3 CDS 85931382 85934387 85931382 ID=cds.MS.gene60222.t1;Parent=MS.gene60222.t1
Gene Sequence

>MS.gene60222

ATGGAAAAGGGAGACTCAACTATTGTTGCATCCGTTTCTGCTGATGGTACGAGTGTTGAGGATAAGAGTGGCAAAGATTCAGGTGAAGCTGATAGTCCCAACGAAGAAAAGTATCCTCAAAATTTTGAGTTGAACGAGGATTTGTTTGTTACAAACACTGTGGAACAAACAAGTGACTGTACTTTGAAGGAGGATCCCATGGTCTTATCTGATCAAGTAGATGGGGATAGTTTGGCTAACGATGAACCTGCAAAAGTGGAATTGGTTGGCATGGAAACTATGGATACGGAATTCGCAACTGAAGACTGTACCTTGAAAAAGGAATATACCATGGTCTCATCTCATCAATTTGATGTGATTATTTTAGAGACTTTCGATGTGGAATTTGCAACTGAAAGCTGTAGTTTGAAGAACGAAGACGATGTGCCTATTTTGGAGACTTTTGACGTGGAATTTGCAACTGAAGGCTGTAGTCTGAAGGACGAAAATGCAGTGATTTCATCTCATCAAATGGATGAGTCTACTTTGGCAAACGATGAACCTGCAAAACTGGCATTGGCGGGTGTGGTAACTTTGGATACGGAATTTGCGACAGAAGGCTCCGTAAAACAAGATTTATCCTATATATCAAAGGCATCGTATCCAGCTGGCGAGGCAGCTATGTCTGCTGACTCAAAATCCTCGTTGTCCAATATCAATATTGGTGGTTCCGGTGCTTGTATGAAGGAGGCTTTAACTAAAAGGTATGCTCCTCGTAAAAAAGTTGCAGTGTTCAGAGATTTTCCAAGTTTATGTGGAGGCAATGCTCCCCGTCTCAGTCAGGATGAGTGCCGAAAGGAGCTTTCTTCTTTGAAAAAAGTTGCTGCCACTGACGTAAAAGAAGCAGAAAATAACAAGAGAAAGTTTGATAACACTGTTCAAGATGATTCTGAAGGGAACACTAAACTTCCTGAAAAACATAATCATCATCATCATGAGGTTAACATAAATTCTAAGGAAGTGGTTAAAGCGGAAAACATGAACACTGTAAAACTTAAAAGTAGTTCCAAGGTCTCATCAGGCAGGAAAGTAGTTCAAGGTTTGATGGCAGTGGTTAAAGAGGAAAACATGAACACTGTAAAACTCAAAAGTAGTTCCAAGGTCTCATCGGGCAGGAAAGTAGGTTTGATGTCTAAATCAGGACGGAACGAAAAGGAAAGAAAGGGAAAGAAAATTGATAATGTCCAGCTGGACAGGTCTAAAACTGCTACCAAGTCGAAAGGTGTACTGAATCATTCTGGGCAGCAGCCGTTGAAAAAGAAAAGAGAAAATTGTAGTTCTGATGACATGGGTCAATTAGTAACGTTAGAGAAGAGTTCTCAAGACCCTAATGAAAATAACAAACACTTTAAAAGTGTCCCAAAATCACATAGTTCGAGCGTAAATGTTTTTCCTCTTGGTCGTAGTAATTTGAGTGGTCATGAGAATGACTCAGTGGCCAGGAATAAAGTGAGGAAAACATTGCGCCTGTTCCATGCTTTTCATAGAAAGATTTTGCAGGAAGCCGAAGCAAAACCAAAGTCAAATATGAAAGAAGTAAAGAGGTTTGATTTACAAGCTGCGAAAAAGCTTAAGGAGGAAGGGAGCTGTGTTAATGAAGGGGAGAATATCTTGGGATCTGTTCCAGGGGTTGAAGTTGGTGATGAATTTCAGTTCAGAATAGAGCTTAATATAATTGGCCTTCATCGCCAGATTCAGGGTGGTATAGATTATGTGAAACAAAAAGACAAGATCCTTGCAACTAGTATTGTTTCTTCGGGGGGCTATGCTGATGACTTAGATAACTCAGATGTTTTGATATATACAGGGCAGGGTGGAAATGTGATGAGCAGTGATAAAGAGCCAGAAGATCAAAAGCTTGAAAGGGGAAATCTTGCTTTGAAGAACAGTAGTGAGGTAAAGAATTCTGTTAGAGTGATACGGGGCTATGAATCAATGGATGGTAAAAGAAAGATATATGTTTATGATGGACTTTATGTAGTTGAGTCATGTTGGCAGGATATAGGGCCACGTGGGAAGCTGGTTTATAAGTTTAGCTTGCGAAGAATCCCGGGTCAACCAGAGCTTGGCTTGAAGGAAGTGAAGAAATTGAAAAAGTTCAAAACAAGAGAAGGTCTATGTGTCGAAGATATTTCCTATGGGAAAGAGAAAATTCCGATATGTGCTGTGAACACCATAGATAACGAGAAACCCCCTACATTTAAATACATAACCGAGATGATATATCCTGAATGTTGCAATCTTATCCCTCCAAAAGGATGTAATTGTACTAATGGATGCTCTGACCATAAGAAATGTTGTTGCGTACTCAAAAATGGAGGGGAGATTCCATTTAATCATAATGGGGATATTGTAGAAGTAAAGCCTCTAGTCTATGAGTGTGGTCCTAAATGCAAGTGCCCTTCAACCTGCCATAATAGAGTTAGCCAACTTGGAATAAATATTCAACTCGAAATTTTTAAAACCAATTCAATGGGATGGGGTGTAAGATCCTTAAATTCCATCCCTTCAGGAAGTTTTATCTGTGAATATATTGGCGAGGTTCTTGAAGAAAAGGAAGCTGAACAAAGAACCGGTAATGATGAGTATCTTTTCGATATCGGAAATAACAAAAACAACAATAATCTTTGTGATGGACTTTCAACCCTTTTTCCAGATTCTGACTCAAGTTCAAGTGAAGTAGTGGAGGATTCTGACTTCACTATCGATGCCGCTCAGTTTGGTAATGTGGGGAGATTTGTTAACCATAGTTGCTCCCCTAATCTTTATGCTCAGAATGTCCTTTATGATCATCAAGACAGCAGGGTTCCTCACATCATGTTATTTGCTGCTGAGAACATTCCTCCCTTGCAAGAGCTGACATACGATTACAATTACACGATAGATACAGTTCGTGATTCCGATGGCAATATCAAAAAGAAGTACTGCTTTTGTGGTTCTGTGGAATGTACTGGTAGGTTGTATTGA
Protein sequence

>MS.gene60222.t1

MEKGDSTIVASVSADGTSVEDKSGKDSGEADSPNEEKYPQNFELNEDLFVTNTVEQTSDCTLKEDPMVLSDQVDGDSLANDEPAKVELVGMETMDTEFATEDCTLKKEYTMVSSHQFDVIILETFDVEFATESCSLKNEDDVPILETFDVEFATEGCSLKDENAVISSHQMDESTLANDEPAKLALAGVVTLDTEFATEGSVKQDLSYISKASYPAGEAAMSADSKSSLSNINIGGSGACMKEALTKRYAPRKKVAVFRDFPSLCGGNAPRLSQDECRKELSSLKKVAATDVKEAENNKRKFDNTVQDDSEGNTKLPEKHNHHHHEVNINSKEVVKAENMNTVKLKSSSKVSSGRKVVQGLMAVVKEENMNTVKLKSSSKVSSGRKVGLMSKSGRNEKERKGKKIDNVQLDRSKTATKSKGVLNHSGQQPLKKKRENCSSDDMGQLVTLEKSSQDPNENNKHFKSVPKSHSSSVNVFPLGRSNLSGHENDSVARNKVRKTLRLFHAFHRKILQEAEAKPKSNMKEVKRFDLQAAKKLKEEGSCVNEGENILGSVPGVEVGDEFQFRIELNIIGLHRQIQGGIDYVKQKDKILATSIVSSGGYADDLDNSDVLIYTGQGGNVMSSDKEPEDQKLERGNLALKNSSEVKNSVRVIRGYESMDGKRKIYVYDGLYVVESCWQDIGPRGKLVYKFSLRRIPGQPELGLKEVKKLKKFKTREGLCVEDISYGKEKIPICAVNTIDNEKPPTFKYITEMIYPECCNLIPPKGCNCTNGCSDHKKCCCVLKNGGEIPFNHNGDIVEVKPLVYECGPKCKCPSTCHNRVSQLGINIQLEIFKTNSMGWGVRSLNSIPSGSFICEYIGEVLEEKEAEQRTGNDEYLFDIGNNKNNNNLCDGLSTLFPDSDSSSSEVVEDSDFTIDAAQFGNVGRFVNHSCSPNLYAQNVLYDHQDSRVPHIMLFAAENIPPLQELTYDYNYTIDTVRDSDGNIKKKYCFCGSVECTGRLY